//plasmid/construction
type: Collection
persistent identity: plasmid construction
display id: plasmid_construction
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_I51001
member: BB base ve
member: BBa_K394001
member: BBa_K243031
member: BBa_I51000
member: BBa_K319043
member: BBa_K394002
member: BBa_I51019
member: BBa_K1404007
member: BBa_K1404006
member: BBa_K1404008
member: BBa_K777133

BBa_K394001
type: ComponentDefinition
persistent identity: BBa K394001
display id: BBa_K394001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plasmid for Chromosomal Integration in Yeast at His3
creator: Brian Landry
created: 2010-10-19T11:00:00Z
modified: 2015-05-08T01:12:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _504_
m user id: 0
owner id: 6327
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This part is designed to allow simple chromosomal integration of BioBrick plasmids. It is based off of the bacterial pSB1AT3 backbone allowing for normal DNA manipulation to occur in bacteria. However the BioBrick cut sites are flanked by homologous regions to the His3 locus in S. cerevisiae. To transform S. cerevisiae the plasmid is cut with BbsI creating two non-sticky ends. The linear fragment then contains His3 homologous sequences at its ends that can be used to chromosomally integrate the BioBrick plasmid into the yeast genome. Transformants can then be selected for using His- medium.
mutable notes: None
mutable provenance: This part was derived from plasmid pSB1AT3. Two successive rounds of PCR inserted a BbsI cut site and 40 bp homologous region in before the prefix and after the suffix. The following Primers were used: Forward 1 GCACAGAACAATAACCTGCTGGAAACGAAGATAAATCgaagacGATTACTTCGCGTTATGCAGGC Reverse 1 GCATCTTCTCAAATATGCTTCCCAGCCTGCTTATCcttctgAAATTCTGCCTCGTGATACGCC Forward 2 tactagtagcggccgctgcagTCTTAACCCAACTGCACAGAACAATAACCTGCTGGAAACG Reverse 2 ctctagaagcggccgcgaattcTTAGTATTGCTGGCCGCATCTTCTCAAATATGCTTCCCAGCC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Tet-R
sequence annotation: VF2
sequence annotation: rep (pMB1) (rev)
sequence annotation: BioBrick Prefix
sequence annotation: VR
sequence annotation: BbsI Cut Site
sequence annotation: His3 homology
sequence annotation: AmpR (rev)
sequence annotation: BioBrick Suffix
sequence annotation: ORI
sequence: https://synbiohub.org/public/igem/BBa_K394001_sequence/1

Tet-R
type: SequenceAnnotation
persistent identity: annotation2102637
display id: annotation2102637
version: 1
top level: BBa_K394001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2102637/range2102637/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2102637/range2102637/1
type: Range
persistent identity: range2102637
display id: range2102637
version: 1
direction: direction/forward
top level: BBa_K394001
start: 1127
end: 2317
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2102640
display id: annotation2102640
version: 1
top level: BBa_K394001
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2102640/range2102640/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2102640/range2102640/1
type: Range
persistent identity: range2102640
display id: range2102640
version: 1
direction: direction/forward
top level: BBa_K394001
start: 3307
end: 3326
orientation: inline

rep (pMB1) (rev)
type: SequenceAnnotation
persistent identity: annotation2102598
display id: annotation2102598
version: 1
top level: BBa_K394001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2102598/range2102598/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2102598/range2102598/1
type: Range
persistent identity: range2102598
display id: range2102598
version: 1
direction: direction/reverse
top level: BBa_K394001
start: 261
end: 875
orientation: inline

BioBrick Prefix
type: SequenceAnnotation
persistent identity: annotation2102649
display id: annotation2102649
version: 1
top level: BBa_K394001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2102649/range2102649/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2102649/range2102649/1
type: Range
persistent identity: range2102649
display id: range2102649
version: 1
direction: direction/forward
top level: BBa_K394001
start: 3435
end: 3457
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2102390
display id: annotation2102390
version: 1
top level: BBa_K394001
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2102390/range2102390/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2102390/range2102390/1
type: Range
persistent identity: range2102390
display id: range2102390
version: 1
direction: direction/reverse
top level: BBa_K394001
start: 157
end: 176
orientation: inline

BbsI Cut Site
type: SequenceAnnotation
persistent identity: annotation2104028
display id: annotation2104028
version: 1
top level: BBa_K394001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2104028/range2104028/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2104028/range2104028/1
type: Range
persistent identity: range2104028
display id: range2104028
version: 1
direction: direction/reverse
top level: BBa_K394001
start: 3379
end: 3385
orientation: inline

His3 homology
type: SequenceAnnotation
persistent identity: annotation2102645
display id: annotation2102645
version: 1
top level: BBa_K394001
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2102645/range2102645/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2102645/range2102645/1
type: Range
persistent identity: range2102645
display id: range2102645
version: 1
direction: direction/forward
top level: BBa_K394001
start: 3386
end: 3435
orientation: inline

BbsI Cut Site
type: SequenceAnnotation
persistent identity: annotation2104026
display id: annotation2104026
version: 1
top level: BBa_K394001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2104026/range2104026/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2104026/range2104026/1
type: Range
persistent identity: range2104026
display id: range2104026
version: 1
direction: direction/forward
top level: BBa_K394001
start: 71
end: 76
orientation: inline

His3 homology
type: SequenceAnnotation
persistent identity: annotation2102389
display id: annotation2102389
version: 1
top level: BBa_K394001
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2102389/range2102389/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2102389/range2102389/1
type: Range
persistent identity: range2102389
display id: range2102389
version: 1
direction: direction/forward
top level: BBa_K394001
start: 22
end: 69
orientation: inline

AmpR (rev)
type: SequenceAnnotation
persistent identity: annotation2102638
display id: annotation2102638
version: 1
top level: BBa_K394001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2102638/range2102638/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2102638/range2102638/1
type: Range
persistent identity: range2102638
display id: range2102638
version: 1
direction: direction/reverse
top level: BBa_K394001
start: 2324
end: 3184
orientation: inline

BioBrick Suffix
type: SequenceAnnotation
persistent identity: annotation2090942
display id: annotation2090942
version: 1
top level: BBa_K394001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2090942/range2090942/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2090942/range2090942/1
type: Range
persistent identity: range2090942
display id: range2090942
version: 1
direction: direction/forward
top level: BBa_K394001
start: 1
end: 21
orientation: inline

ORI
type: SequenceAnnotation
persistent identity: annotation2102635
display id: annotation2102635
version: 1
top level: BBa_K394001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2102635/range2102635/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2102635/range2102635/1
type: Range
persistent identity: range2102635
display id: range2102635
version: 1
direction: direction/forward
top level: BBa_K394001
start: 276
end: 276
orientation: inline

BBa_K243031
type: ComponentDefinition
persistent identity: BBa K243031
display id: BBa_K243031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pMA
creator: Freiburg Bioware09
created: 2009-10-19T11:00:00Z
modified: 2015-05-08T01:11:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _352_
m user id: 0
owner id: 4732
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a plasmid that we use for consruction and further cloning steps.
mutable notes: Designed according to RFC25
mutable provenance: Mr. Gene.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence: https://synbiohub.org/public/igem/BBa_K243031_sequence/1

BBa_K777133
type: ComponentDefinition
persistent identity: BBa K777133
display id: BBa_K777133
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Similar to pSB1C3, with RFP reporter
creator: Team Goettingen
created: 2012-09-27T11:00:00Z
modified: 2015-05-08T01:13:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is similar to [http://partsregistry.org/Part:BBa_J61002 J61002] but the backbone is based on pSB1C3 and carries a chloramphenicol resistance.
mutable notes: * No specific design considerations.
mutable provenance: * This part is a compination of E1010, B0034 and pSB1C3 from the registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: rep (pMB1)
sequence annotation: XbaI
sequence annotation: PstI
sequence annotation: VF2 primer binding site
sequence annotation: EcoRI
sequence annotation: SpeI
sequence annotation: mRFP1
sequence annotation: Chloramphenicol resistance
sequence annotation: BB0034
sequence annotation: RBS
sequence annotation: E1010
sequence annotation: VR primer binding site
sequence annotation: ORI
sequence: https://synbiohub.org/public/igem/BBa_K777133_sequence/1

rep (pMB1)
type: SequenceAnnotation
persistent identity: annotation2203655
display id: annotation2203655
version: 1
top level: BBa_K777133
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203655/range2203655/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203655/range2203655/1
type: Range
persistent identity: range2203655
display id: range2203655
version: 1
direction: direction/forward
top level: BBa_K777133
start: 1129
end: 1735
orientation: inline

XbaI
type: SequenceAnnotation
persistent identity: annotation2203668
display id: annotation2203668
version: 1
top level: BBa_K777133
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203668/range2203668/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203668/range2203668/1
type: Range
persistent identity: range2203668
display id: range2203668
version: 1
direction: direction/forward
top level: BBa_K777133
start: 2932
end: 2937
orientation: inline

PstI
type: SequenceAnnotation
persistent identity: annotation2203666
display id: annotation2203666
version: 1
top level: BBa_K777133
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203666/range2203666/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203666/range2203666/1
type: Range
persistent identity: range2203666
display id: range2203666
version: 1
direction: direction/forward
top level: BBa_K777133
start: 884
end: 889
orientation: inline

VF2 primer binding site
type: SequenceAnnotation
persistent identity: annotation2203664
display id: annotation2203664
version: 1
top level: BBa_K777133
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203664/range2203664/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203664/range2203664/1
type: Range
persistent identity: range2203664
display id: range2203664
version: 1
direction: direction/forward
top level: BBa_K777133
start: 2799
end: 2818
orientation: inline

EcoRI
type: SequenceAnnotation
persistent identity: annotation2203667
display id: annotation2203667
version: 1
top level: BBa_K777133
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203667/range2203667/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203667/range2203667/1
type: Range
persistent identity: range2203667
display id: range2203667
version: 1
direction: direction/forward
top level: BBa_K777133
start: 2917
end: 2922
orientation: inline

SpeI
type: SequenceAnnotation
persistent identity: annotation2203665
display id: annotation2203665
version: 1
top level: BBa_K777133
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203665/range2203665/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203665/range2203665/1
type: Range
persistent identity: range2203665
display id: range2203665
version: 1
direction: direction/forward
top level: BBa_K777133
start: 1
end: 6
orientation: inline

mRFP1
type: SequenceAnnotation
persistent identity: annotation2203653
display id: annotation2203653
version: 1
top level: BBa_K777133
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203653/range2203653/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203653/range2203653/1
type: Range
persistent identity: range2203653
display id: range2203653
version: 1
direction: direction/forward
top level: BBa_K777133
start: 26
end: 703
orientation: inline

Chloramphenicol resistance
type: SequenceAnnotation
persistent identity: annotation2203657
display id: annotation2203657
version: 1
top level: BBa_K777133
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203657/range2203657/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203657/range2203657/1
type: Range
persistent identity: range2203657
display id: range2203657
version: 1
direction: direction/reverse
top level: BBa_K777133
start: 2028
end: 2687
orientation: inline

BB0034
type: SequenceAnnotation
persistent identity: annotation2203652
display id: annotation2203652
version: 1
top level: BBa_K777133
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203652/range2203652/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203652/range2203652/1
type: Range
persistent identity: range2203652
display id: range2203652
version: 1
direction: direction/forward
top level: BBa_K777133
start: 8
end: 19
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2203651
display id: annotation2203651
version: 1
top level: BBa_K777133
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203651/range2203651/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203651/range2203651/1
type: Range
persistent identity: range2203651
display id: range2203651
version: 1
direction: direction/forward
top level: BBa_K777133
start: 8
end: 19
orientation: inline

E1010
type: SequenceAnnotation
persistent identity: annotation2203654
display id: annotation2203654
version: 1
top level: BBa_K777133
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203654/range2203654/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203654/range2203654/1
type: Range
persistent identity: range2203654
display id: range2203654
version: 1
direction: direction/forward
top level: BBa_K777133
start: 26
end: 703
orientation: inline

VR primer binding site
type: SequenceAnnotation
persistent identity: annotation2203663
display id: annotation2203663
version: 1
top level: BBa_K777133
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203663/range2203663/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203663/range2203663/1
type: Range
persistent identity: range2203663
display id: range2203663
version: 1
direction: direction/forward
top level: BBa_K777133
start: 1023
end: 1042
orientation: inline

ORI
type: SequenceAnnotation
persistent identity: annotation2203662
display id: annotation2203662
version: 1
top level: BBa_K777133
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203662/range2203662/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203662/range2203662/1
type: Range
persistent identity: range2203662
display id: range2203662
version: 1
direction: direction/forward
top level: BBa_K777133
start: 1144
end: 1144
orientation: inline

BBa_I51000
type: ComponentDefinition
persistent identity: BBa I51000
display id: BBa_I51000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BioBrick base vector, as originally designed
creator: Reshma Shetty
created: 2006-03-13T12:00:00Z
modified: 2015-08-31T04:07:42Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
component: BBa_B0043
component: BBa_G00000
component: BBa_G00001
component: BBa_B0042
component: BBa_B0045
component: BBa_B0044
component: BBa_B0053
component: BBa_G00102
component: BBa_B0062
component: BBa_P1006
component: BBa_I52000
component: BBa_G00100
component: BBa_B0054
component: BBa_B0055
sequence annotation: BBa_G00100
sequence annotation: BBa_B0053
sequence annotation: BBa_B0045
sequence annotation: BBa_G00102
sequence annotation: BBa_G00001
sequence annotation: BBa_B0044
sequence annotation: BBa_B0055
sequence annotation: BBa_I52000
sequence annotation: BBa_B0054
sequence annotation: BBa_P1006
sequence annotation: BBa_B0042
sequence annotation: BBa_B0043
sequence annotation: BBa_B0062
sequence annotation: BBa_G00000
sequence: https://synbiohub.org/public/igem/BBa_I51000_sequence/1

BBa_B0043
type: Component
persistent identity: component2236107
display id: component2236107
version: 1
top level: BBa_I51000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_G00000
type: Component
persistent identity: component2236112
display id: component2236112
version: 1
top level: BBa_I51000
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_G00001
type: Component
persistent identity: component2236060
display id: component2236060
version: 1
top level: BBa_I51000
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_B0042
type: Component
persistent identity: component2236102
display id: component2236102
version: 1
top level: BBa_I51000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_B0045
type: Component
persistent identity: component2236081
display id: component2236081
version: 1
top level: BBa_I51000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0045/1

BBa_B0044
type: Component
persistent identity: component2236065
display id: component2236065
version: 1
top level: BBa_I51000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_B0053
type: Component
persistent identity: component2236090
display id: component2236090
version: 1
top level: BBa_I51000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_B0045
type: Component
persistent identity: component2236088
display id: component2236088
version: 1
top level: BBa_I51000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0045/1

BBa_G00102
type: Component
persistent identity: component2236077
display id: component2236077
version: 1
top level: BBa_I51000
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_B0062
type: Component
persistent identity: component2236079
display id: component2236079
version: 1
top level: BBa_I51000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_P1006
type: Component
persistent identity: component2236086
display id: component2236086
version: 1
top level: BBa_I51000
access: public
definition: https://synbiohub.org/public/igem/BBa_P1006/1

BBa_B0042
type: Component
persistent identity: component2236072
display id: component2236072
version: 1
top level: BBa_I51000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_I52000
type: Component
persistent identity: component2236127
display id: component2236127
version: 1
top level: BBa_I51000
access: public
definition: https://synbiohub.org/public/igem/BBa_I52000/1

BBa_G00100
type: Component
persistent identity: component2236092
display id: component2236092
version: 1
top level: BBa_I51000
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_B0054
type: Component
persistent identity: component2236075
display id: component2236075
version: 1
top level: BBa_I51000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_B0055
type: Component
persistent identity: component2236095
display id: component2236095
version: 1
top level: BBa_I51000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation2236092
display id: annotation2236092
version: 1
top level: BBa_I51000
location: https://synbiohub.org/public/igem/BBa_I51000/annotation2236092/range2236092/1
component: BBa_G00100

https://synbiohub.org/public/igem/BBa_I51000/annotation2236092/range2236092/1
type: Range
persistent identity: range2236092
display id: range2236092
version: 1
direction: direction/reverse
top level: BBa_I51000
start: 1204
end: 1223
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation2236090
display id: annotation2236090
version: 1
top level: BBa_I51000
location: https://synbiohub.org/public/igem/BBa_I51000/annotation2236090/range2236090/1
component: BBa_B0053

https://synbiohub.org/public/igem/BBa_I51000/annotation2236090/range2236090/1
type: Range
persistent identity: range2236090
display id: range2236090
version: 1
direction: direction/reverse
top level: BBa_I51000
start: 1132
end: 1203
orientation: inline

BBa_B0045
type: SequenceAnnotation
persistent identity: annotation2236081
display id: annotation2236081
version: 1
top level: BBa_I51000
location: https://synbiohub.org/public/igem/BBa_I51000/annotation2236081/range2236081/1
component: BBa_B0045

https://synbiohub.org/public/igem/BBa_I51000/annotation2236081/range2236081/1
type: Range
persistent identity: range2236081
display id: range2236081
version: 1
direction: direction/reverse
top level: BBa_I51000
start: 177
end: 182
orientation: inline

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation2236077
display id: annotation2236077
version: 1
top level: BBa_I51000
location: https://synbiohub.org/public/igem/BBa_I51000/annotation2236077/range2236077/1
component: BBa_G00102

https://synbiohub.org/public/igem/BBa_I51000/annotation2236077/range2236077/1
type: Range
persistent identity: range2236077
display id: range2236077
version: 1
direction: direction/reverse
top level: BBa_I51000
start: 116
end: 135
orientation: inline

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation2236060
display id: annotation2236060
version: 1
top level: BBa_I51000
location: https://synbiohub.org/public/igem/BBa_I51000/annotation2236060/range2236060/1
component: BBa_G00001

https://synbiohub.org/public/igem/BBa_I51000/annotation2236060/range2236060/1
type: Range
persistent identity: range2236060
display id: range2236060
version: 1
direction: direction/reverse
top level: BBa_I51000
start: 1
end: 21
orientation: inline

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation2236065
display id: annotation2236065
version: 1
top level: BBa_I51000
location: https://synbiohub.org/public/igem/BBa_I51000/annotation2236065/range2236065/1
component: BBa_B0044

https://synbiohub.org/public/igem/BBa_I51000/annotation2236065/range2236065/1
type: Range
persistent identity: range2236065
display id: range2236065
version: 1
direction: direction/reverse
top level: BBa_I51000
start: 22
end: 34
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation2236095
display id: annotation2236095
version: 1
top level: BBa_I51000
location: https://synbiohub.org/public/igem/BBa_I51000/annotation2236095/range2236095/1
component: BBa_B0055

https://synbiohub.org/public/igem/BBa_I51000/annotation2236095/range2236095/1
type: Range
persistent identity: range2236095
display id: range2236095
version: 1
direction: direction/reverse
top level: BBa_I51000
start: 1224
end: 1301
orientation: inline

BBa_B0045
type: SequenceAnnotation
persistent identity: annotation2236088
display id: annotation2236088
version: 1
top level: BBa_I51000
location: https://synbiohub.org/public/igem/BBa_I51000/annotation2236088/range2236088/1
component: BBa_B0045

https://synbiohub.org/public/igem/BBa_I51000/annotation2236088/range2236088/1
type: Range
persistent identity: range2236088
display id: range2236088
version: 1
direction: direction/reverse
top level: BBa_I51000
start: 1126
end: 1131
orientation: inline

BBa_I52000
type: SequenceAnnotation
persistent identity: annotation2236127
display id: annotation2236127
version: 1
top level: BBa_I51000
location: https://synbiohub.org/public/igem/BBa_I51000/annotation2236127/range2236127/1
component: BBa_I52000

https://synbiohub.org/public/igem/BBa_I51000/annotation2236127/range2236127/1
type: Range
persistent identity: range2236127
display id: range2236127
version: 1
direction: direction/reverse
top level: BBa_I51000
start: 1349
end: 2446
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation2236075
display id: annotation2236075
version: 1
top level: BBa_I51000
location: https://synbiohub.org/public/igem/BBa_I51000/annotation2236075/range2236075/1
component: BBa_B0054

https://synbiohub.org/public/igem/BBa_I51000/annotation2236075/range2236075/1
type: Range
persistent identity: range2236075
display id: range2236075
version: 1
direction: direction/reverse
top level: BBa_I51000
start: 47
end: 115
orientation: inline

BBa_P1006
type: SequenceAnnotation
persistent identity: annotation2236086
display id: annotation2236086
version: 1
top level: BBa_I51000
location: https://synbiohub.org/public/igem/BBa_I51000/annotation2236086/range2236086/1
component: BBa_P1006

https://synbiohub.org/public/igem/BBa_I51000/annotation2236086/range2236086/1
type: Range
persistent identity: range2236086
display id: range2236086
version: 1
direction: direction/reverse
top level: BBa_I51000
start: 183
end: 1125
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2236072
display id: annotation2236072
version: 1
top level: BBa_I51000
location: https://synbiohub.org/public/igem/BBa_I51000/annotation2236072/range2236072/1
component: BBa_B0042

https://synbiohub.org/public/igem/BBa_I51000/annotation2236072/range2236072/1
type: Range
persistent identity: range2236072
display id: range2236072
version: 1
direction: direction/reverse
top level: BBa_I51000
start: 35
end: 46
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2236102
display id: annotation2236102
version: 1
top level: BBa_I51000
location: https://synbiohub.org/public/igem/BBa_I51000/annotation2236102/range2236102/1
component: BBa_B0042

https://synbiohub.org/public/igem/BBa_I51000/annotation2236102/range2236102/1
type: Range
persistent identity: range2236102
display id: range2236102
version: 1
direction: direction/reverse
top level: BBa_I51000
start: 1302
end: 1313
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation2236107
display id: annotation2236107
version: 1
top level: BBa_I51000
location: https://synbiohub.org/public/igem/BBa_I51000/annotation2236107/range2236107/1
component: BBa_B0043

https://synbiohub.org/public/igem/BBa_I51000/annotation2236107/range2236107/1
type: Range
persistent identity: range2236107
display id: range2236107
version: 1
direction: direction/reverse
top level: BBa_I51000
start: 1314
end: 1326
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation2236079
display id: annotation2236079
version: 1
top level: BBa_I51000
location: https://synbiohub.org/public/igem/BBa_I51000/annotation2236079/range2236079/1
component: BBa_B0062

https://synbiohub.org/public/igem/BBa_I51000/annotation2236079/range2236079/1
type: Range
persistent identity: range2236079
display id: range2236079
version: 1
direction: direction/reverse
top level: BBa_I51000
start: 136
end: 176
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation2236112
display id: annotation2236112
version: 1
top level: BBa_I51000
location: https://synbiohub.org/public/igem/BBa_I51000/annotation2236112/range2236112/1
component: BBa_G00000

https://synbiohub.org/public/igem/BBa_I51000/annotation2236112/range2236112/1
type: Range
persistent identity: range2236112
display id: range2236112
version: 1
direction: direction/reverse
top level: BBa_I51000
start: 1327
end: 1348
orientation: inline

BBa_K1404007
type: ComponentDefinition
persistent identity: BBa K1404007
display id: BBa_K1404007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: p70-CsgA-His, His-tagged curli generator
creator: Juliette Paillet, Alexandre Duprey, Emiel van der Kouwe
created: 2014-09-23T11:00:00Z
modified: 2015-05-08T01:10:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1782_
m user id: 0
owner id: 21195
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: temp
mutable notes: temp
mutable provenance: temp
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: BBa_K342000
sequence annotation: Beta sheet3
sequence annotation: His tag
sequence annotation: Beta sheet2
sequence annotation: CsgG signal
sequence annotation: Linker
sequence annotation: CsgA-His1
sequence annotation: Beta sheet 1
sequence annotation: GCT into GCC
sequence annotation: Beta sheet5
sequence annotation: Beta sheet4
sequence annotation: RBS Paris 2009
sequence annotation: Sec signal
sequence: https://synbiohub.org/public/igem/BBa_K1404007_sequence/1

BBa_K342000
type: SequenceAnnotation
persistent identity: annotation2426218
display id: annotation2426218
version: 1
top level: BBa_K1404007
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426218/range2426218/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426218/range2426218/1
type: Range
persistent identity: range2426218
display id: range2426218
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 1
end: 70
orientation: inline

Beta sheet3
type: SequenceAnnotation
persistent identity: annotation2426225
display id: annotation2426225
version: 1
top level: BBa_K1404007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426225/range2426225/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426225/range2426225/1
type: Range
persistent identity: range2426225
display id: range2426225
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 353
end: 421
orientation: inline

His tag
type: SequenceAnnotation
persistent identity: annotation2426230
display id: annotation2426230
version: 1
top level: BBa_K1404007
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426230/range2426230/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426230/range2426230/1
type: Range
persistent identity: range2426230
display id: range2426230
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 557
end: 574
orientation: inline

Beta sheet2
type: SequenceAnnotation
persistent identity: annotation2426224
display id: annotation2426224
version: 1
top level: BBa_K1404007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426224/range2426224/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426224/range2426224/1
type: Range
persistent identity: range2426224
display id: range2426224
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 287
end: 352
orientation: inline

CsgG signal
type: SequenceAnnotation
persistent identity: annotation2426222
display id: annotation2426222
version: 1
top level: BBa_K1404007
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426222/range2426222/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426222/range2426222/1
type: Range
persistent identity: range2426222
display id: range2426222
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 152
end: 217
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2426229
display id: annotation2426229
version: 1
top level: BBa_K1404007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426229/range2426229/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426229/range2426229/1
type: Range
persistent identity: range2426229
display id: range2426229
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 545
end: 556
orientation: inline

CsgA-His1
type: SequenceAnnotation
persistent identity: annotation2426219
display id: annotation2426219
version: 1
top level: BBa_K1404007
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426219/range2426219/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426219/range2426219/1
type: Range
persistent identity: range2426219
display id: range2426219
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 92
end: 577
orientation: inline

Beta sheet 1
type: SequenceAnnotation
persistent identity: annotation2426223
display id: annotation2426223
version: 1
top level: BBa_K1404007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426223/range2426223/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426223/range2426223/1
type: Range
persistent identity: range2426223
display id: range2426223
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 218
end: 286
orientation: inline

GCT into GCC
type: SequenceAnnotation
persistent identity: annotation2426227
display id: annotation2426227
version: 1
top level: BBa_K1404007
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426227/range2426227/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426227/range2426227/1
type: Range
persistent identity: range2426227
display id: range2426227
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 461
end: 463
orientation: inline

Beta sheet5
type: SequenceAnnotation
persistent identity: annotation2426228
display id: annotation2426228
version: 1
top level: BBa_K1404007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426228/range2426228/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426228/range2426228/1
type: Range
persistent identity: range2426228
display id: range2426228
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 488
end: 544
orientation: inline

Beta sheet4
type: SequenceAnnotation
persistent identity: annotation2426226
display id: annotation2426226
version: 1
top level: BBa_K1404007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426226/range2426226/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426226/range2426226/1
type: Range
persistent identity: range2426226
display id: range2426226
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 422
end: 487
orientation: inline

RBS Paris 2009
type: SequenceAnnotation
persistent identity: annotation2426220
display id: annotation2426220
version: 1
top level: BBa_K1404007
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426220/range2426220/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426220/range2426220/1
type: Range
persistent identity: range2426220
display id: range2426220
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 79
end: 91
orientation: inline

Sec signal
type: SequenceAnnotation
persistent identity: annotation2426221
display id: annotation2426221
version: 1
top level: BBa_K1404007
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426221/range2426221/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426221/range2426221/1
type: Range
persistent identity: range2426221
display id: range2426221
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 92
end: 151
orientation: inline

BBa_K1404008
type: ComponentDefinition
persistent identity: BBa K1404008
display id: BBa_K1404008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: p70-CsgA-His*2, double His-tagged curli generator
creator: Juliette Paillet, Alexandre Duprey, Emiel van der Kouwe
created: 2014-09-23T11:00:00Z
modified: 2015-05-08T01:10:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1782_
m user id: 0
owner id: 21195
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: temp
mutable notes: temp
mutable provenance: temp
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: GCT into GCC
sequence annotation: SEC signal
sequence annotation: Beta sheet
sequence annotation: Beta sheet5
sequence annotation: RBS Paris 2009
sequence annotation: Linker
sequence annotation: CsgD signal
sequence annotation: BBa_K342000
sequence annotation: His tag
sequence annotation: CsgA-His2
sequence annotation: Beta sheet4
sequence annotation: Beta sheet2
sequence annotation: linker
sequence: https://synbiohub.org/public/igem/BBa_K1404008_sequence/1

GCT into GCC
type: SequenceAnnotation
persistent identity: annotation2426516
display id: annotation2426516
version: 1
top level: BBa_K1404008
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426516/range2426516/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426516/range2426516/1
type: Range
persistent identity: range2426516
display id: range2426516
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 461
end: 463
orientation: inline

SEC signal
type: SequenceAnnotation
persistent identity: annotation2426509
display id: annotation2426509
version: 1
top level: BBa_K1404008
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426509/range2426509/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426509/range2426509/1
type: Range
persistent identity: range2426509
display id: range2426509
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 92
end: 151
orientation: inline

Beta sheet
type: SequenceAnnotation
persistent identity: annotation2426512
display id: annotation2426512
version: 1
top level: BBa_K1404008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426512/range2426512/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426512/range2426512/1
type: Range
persistent identity: range2426512
display id: range2426512
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 218
end: 286
orientation: inline

Beta sheet5
type: SequenceAnnotation
persistent identity: annotation2426517
display id: annotation2426517
version: 1
top level: BBa_K1404008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426517/range2426517/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426517/range2426517/1
type: Range
persistent identity: range2426517
display id: range2426517
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 488
end: 544
orientation: inline

RBS Paris 2009
type: SequenceAnnotation
persistent identity: annotation2426508
display id: annotation2426508
version: 1
top level: BBa_K1404008
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426508/range2426508/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426508/range2426508/1
type: Range
persistent identity: range2426508
display id: range2426508
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 79
end: 91
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2426518
display id: annotation2426518
version: 1
top level: BBa_K1404008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426518/range2426518/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426518/range2426518/1
type: Range
persistent identity: range2426518
display id: range2426518
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 545
end: 556
orientation: inline

CsgD signal
type: SequenceAnnotation
persistent identity: annotation2426511
display id: annotation2426511
version: 1
top level: BBa_K1404008
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426511/range2426511/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426511/range2426511/1
type: Range
persistent identity: range2426511
display id: range2426511
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 152
end: 217
orientation: inline

BBa_K342000
type: SequenceAnnotation
persistent identity: annotation2426507
display id: annotation2426507
version: 1
top level: BBa_K1404008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426507/range2426507/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426507/range2426507/1
type: Range
persistent identity: range2426507
display id: range2426507
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 1
end: 70
orientation: inline

His tag
type: SequenceAnnotation
persistent identity: annotation2426521
display id: annotation2426521
version: 1
top level: BBa_K1404008
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426521/range2426521/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426521/range2426521/1
type: Range
persistent identity: range2426521
display id: range2426521
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 587
end: 604
orientation: inline

Beta sheet
type: SequenceAnnotation
persistent identity: annotation2426514
display id: annotation2426514
version: 1
top level: BBa_K1404008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426514/range2426514/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426514/range2426514/1
type: Range
persistent identity: range2426514
display id: range2426514
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 353
end: 421
orientation: inline

CsgA-His2
type: SequenceAnnotation
persistent identity: annotation2426510
display id: annotation2426510
version: 1
top level: BBa_K1404008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426510/range2426510/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426510/range2426510/1
type: Range
persistent identity: range2426510
display id: range2426510
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 92
end: 607
orientation: inline

His tag
type: SequenceAnnotation
persistent identity: annotation2426519
display id: annotation2426519
version: 1
top level: BBa_K1404008
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426519/range2426519/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426519/range2426519/1
type: Range
persistent identity: range2426519
display id: range2426519
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 557
end: 574
orientation: inline

Beta sheet4
type: SequenceAnnotation
persistent identity: annotation2426515
display id: annotation2426515
version: 1
top level: BBa_K1404008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426515/range2426515/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426515/range2426515/1
type: Range
persistent identity: range2426515
display id: range2426515
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 422
end: 487
orientation: inline

Beta sheet2
type: SequenceAnnotation
persistent identity: annotation2426513
display id: annotation2426513
version: 1
top level: BBa_K1404008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426513/range2426513/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426513/range2426513/1
type: Range
persistent identity: range2426513
display id: range2426513
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 287
end: 352
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation2426520
display id: annotation2426520
version: 1
top level: BBa_K1404008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426520/range2426520/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426520/range2426520/1
type: Range
persistent identity: range2426520
display id: range2426520
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 575
end: 586
orientation: inline

BBa_I51001
type: ComponentDefinition
persistent identity: BBa I51001
display id: BBa_I51001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BioBrick base vector, as revised by Codon Devices
creator: Reshma Shetty
created: 2007-01-29T12:00:00Z
modified: 2015-08-31T04:07:42Z
discontinued: true
dominant: false
experience: experience/Issues
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: This is a vector scaffold for constructing new BioBricks vectors. Information on the design is available at http://openwetware.org/wiki/Synthetic_Biology:Vectors/pSB%2A%2A5_design
mutable notes: Documented at http://openwetware.org/wiki/Synthetic_Biology:Vectors/pSB%2A%2A5_design Due to technical difficulties during synthesis, rather than including BBa_P1011 as designed, BBa_P1016 was synthesized as a component of this part. Hence, BBa_I51000 documents the original design and BBa_I51001 is the vector scaffold as synthesized.
mutable provenance: This is a composite part generated from several basic parts obtained from a variety of sources.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
component: BBa_B0042
component: BBa_G00001
component: BBa_G00102
component: BBa_G00100
component: BBa_B0043
component: BBa_B0045
component: BBa_B0044
component: BBa_P1006
component: BBa_B0055
component: BBa_B0053
component: BBa_P1016
component: BBa_B0062
component: BBa_B0054
component: BBa_I50020
component: BBa_G00000
sequence annotation: BBa_P1016
sequence annotation: BBa_P1006
sequence annotation: BBa_B0055
sequence annotation: BBa_G00000
sequence annotation: BBa_B0042
sequence annotation: BBa_G00100
sequence annotation: BBa_B0043
sequence annotation: BBa_I50020
sequence annotation: BBa_B0062
sequence annotation: BBa_B0054
sequence annotation: BBa_B0053
sequence annotation: BBa_B0045
sequence annotation: BBa_G00001
sequence annotation: BBa_B0044
sequence annotation: BBa_G00102
sequence: https://synbiohub.org/public/igem/BBa_I51001_sequence/1

BBa_B0042
type: Component
persistent identity: component1910705
display id: component1910705
version: 1
top level: BBa_I51001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_G00001
type: Component
persistent identity: component1910693
display id: component1910693
version: 1
top level: BBa_I51001
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_G00102
type: Component
persistent identity: component1910709
display id: component1910709
version: 1
top level: BBa_I51001
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_G00100
type: Component
persistent identity: component1910723
display id: component1910723
version: 1
top level: BBa_I51001
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_B0043
type: Component
persistent identity: component1910738
display id: component1910738
version: 1
top level: BBa_I51001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_B0042
type: Component
persistent identity: component1910733
display id: component1910733
version: 1
top level: BBa_I51001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_B0045
type: Component
persistent identity: component1910720
display id: component1910720
version: 1
top level: BBa_I51001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0045/1

BBa_B0044
type: Component
persistent identity: component1910698
display id: component1910698
version: 1
top level: BBa_I51001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_P1006
type: Component
persistent identity: component1910718
display id: component1910718
version: 1
top level: BBa_I51001
access: public
definition: https://synbiohub.org/public/igem/BBa_P1006/1

BBa_B0055
type: Component
persistent identity: component1910726
display id: component1910726
version: 1
top level: BBa_I51001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_B0053
type: Component
persistent identity: component1910722
display id: component1910722
version: 1
top level: BBa_I51001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_P1016
type: Component
persistent identity: component1910744
display id: component1910744
version: 1
top level: BBa_I51001
access: public
definition: https://synbiohub.org/public/igem/BBa_P1016/1

BBa_B0062
type: Component
persistent identity: component1910711
display id: component1910711
version: 1
top level: BBa_I51001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_B0054
type: Component
persistent identity: component1910708
display id: component1910708
version: 1
top level: BBa_I51001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_I50020
type: Component
persistent identity: component1910759
display id: component1910759
version: 1
top level: BBa_I51001
access: public
definition: https://synbiohub.org/public/igem/BBa_I50020/1

BBa_B0045
type: Component
persistent identity: component1910713
display id: component1910713
version: 1
top level: BBa_I51001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0045/1

BBa_G00000
type: Component
persistent identity: component1910740
display id: component1910740
version: 1
top level: BBa_I51001
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_P1016
type: SequenceAnnotation
persistent identity: annotation1910744
display id: annotation1910744
version: 1
top level: BBa_I51001
location: https://synbiohub.org/public/igem/BBa_I51001/annotation1910744/range1910744/1
component: BBa_P1016

https://synbiohub.org/public/igem/BBa_I51001/annotation1910744/range1910744/1
type: Range
persistent identity: range1910744
display id: range1910744
version: 1
direction: direction/reverse
top level: BBa_I51001
start: 1349
end: 1764
orientation: inline

BBa_P1006
type: SequenceAnnotation
persistent identity: annotation1910718
display id: annotation1910718
version: 1
top level: BBa_I51001
location: https://synbiohub.org/public/igem/BBa_I51001/annotation1910718/range1910718/1
component: BBa_P1006

https://synbiohub.org/public/igem/BBa_I51001/annotation1910718/range1910718/1
type: Range
persistent identity: range1910718
display id: range1910718
version: 1
direction: direction/reverse
top level: BBa_I51001
start: 183
end: 1125
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation1910726
display id: annotation1910726
version: 1
top level: BBa_I51001
location: https://synbiohub.org/public/igem/BBa_I51001/annotation1910726/range1910726/1
component: BBa_B0055

https://synbiohub.org/public/igem/BBa_I51001/annotation1910726/range1910726/1
type: Range
persistent identity: range1910726
display id: range1910726
version: 1
direction: direction/reverse
top level: BBa_I51001
start: 1224
end: 1301
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation1910740
display id: annotation1910740
version: 1
top level: BBa_I51001
location: https://synbiohub.org/public/igem/BBa_I51001/annotation1910740/range1910740/1
component: BBa_G00000

https://synbiohub.org/public/igem/BBa_I51001/annotation1910740/range1910740/1
type: Range
persistent identity: range1910740
display id: range1910740
version: 1
direction: direction/reverse
top level: BBa_I51001
start: 1327
end: 1348
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation1910705
display id: annotation1910705
version: 1
top level: BBa_I51001
location: https://synbiohub.org/public/igem/BBa_I51001/annotation1910705/range1910705/1
component: BBa_B0042

https://synbiohub.org/public/igem/BBa_I51001/annotation1910705/range1910705/1
type: Range
persistent identity: range1910705
display id: range1910705
version: 1
direction: direction/reverse
top level: BBa_I51001
start: 35
end: 46
orientation: inline

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation1910723
display id: annotation1910723
version: 1
top level: BBa_I51001
location: https://synbiohub.org/public/igem/BBa_I51001/annotation1910723/range1910723/1
component: BBa_G00100

https://synbiohub.org/public/igem/BBa_I51001/annotation1910723/range1910723/1
type: Range
persistent identity: range1910723
display id: range1910723
version: 1
direction: direction/reverse
top level: BBa_I51001
start: 1204
end: 1223
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation1910738
display id: annotation1910738
version: 1
top level: BBa_I51001
location: https://synbiohub.org/public/igem/BBa_I51001/annotation1910738/range1910738/1
component: BBa_B0043

https://synbiohub.org/public/igem/BBa_I51001/annotation1910738/range1910738/1
type: Range
persistent identity: range1910738
display id: range1910738
version: 1
direction: direction/reverse
top level: BBa_I51001
start: 1314
end: 1326
orientation: inline

BBa_I50020
type: SequenceAnnotation
persistent identity: annotation1910759
display id: annotation1910759
version: 1
top level: BBa_I51001
location: https://synbiohub.org/public/igem/BBa_I51001/annotation1910759/range1910759/1
component: BBa_I50020

https://synbiohub.org/public/igem/BBa_I51001/annotation1910759/range1910759/1
type: Range
persistent identity: range1910759
display id: range1910759
version: 1
direction: direction/reverse
top level: BBa_I51001
start: 1765
end: 2438
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation1910711
display id: annotation1910711
version: 1
top level: BBa_I51001
location: https://synbiohub.org/public/igem/BBa_I51001/annotation1910711/range1910711/1
component: BBa_B0062

https://synbiohub.org/public/igem/BBa_I51001/annotation1910711/range1910711/1
type: Range
persistent identity: range1910711
display id: range1910711
version: 1
direction: direction/reverse
top level: BBa_I51001
start: 136
end: 176
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation1910708
display id: annotation1910708
version: 1
top level: BBa_I51001
location: https://synbiohub.org/public/igem/BBa_I51001/annotation1910708/range1910708/1
component: BBa_B0054

https://synbiohub.org/public/igem/BBa_I51001/annotation1910708/range1910708/1
type: Range
persistent identity: range1910708
display id: range1910708
version: 1
direction: direction/reverse
top level: BBa_I51001
start: 47
end: 115
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation1910722
display id: annotation1910722
version: 1
top level: BBa_I51001
location: https://synbiohub.org/public/igem/BBa_I51001/annotation1910722/range1910722/1
component: BBa_B0053

https://synbiohub.org/public/igem/BBa_I51001/annotation1910722/range1910722/1
type: Range
persistent identity: range1910722
display id: range1910722
version: 1
direction: direction/reverse
top level: BBa_I51001
start: 1132
end: 1203
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation1910733
display id: annotation1910733
version: 1
top level: BBa_I51001
location: https://synbiohub.org/public/igem/BBa_I51001/annotation1910733/range1910733/1
component: BBa_B0042

https://synbiohub.org/public/igem/BBa_I51001/annotation1910733/range1910733/1
type: Range
persistent identity: range1910733
display id: range1910733
version: 1
direction: direction/reverse
top level: BBa_I51001
start: 1302
end: 1313
orientation: inline

BBa_B0045
type: SequenceAnnotation
persistent identity: annotation1910713
display id: annotation1910713
version: 1
top level: BBa_I51001
location: https://synbiohub.org/public/igem/BBa_I51001/annotation1910713/range1910713/1
component: BBa_B0045

https://synbiohub.org/public/igem/BBa_I51001/annotation1910713/range1910713/1
type: Range
persistent identity: range1910713
display id: range1910713
version: 1
direction: direction/reverse
top level: BBa_I51001
start: 177
end: 182
orientation: inline

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation1910693
display id: annotation1910693
version: 1
top level: BBa_I51001
location: https://synbiohub.org/public/igem/BBa_I51001/annotation1910693/range1910693/1
component: BBa_G00001

https://synbiohub.org/public/igem/BBa_I51001/annotation1910693/range1910693/1
type: Range
persistent identity: range1910693
display id: range1910693
version: 1
direction: direction/reverse
top level: BBa_I51001
start: 1
end: 21
orientation: inline

BBa_B0045
type: SequenceAnnotation
persistent identity: annotation1910720
display id: annotation1910720
version: 1
top level: BBa_I51001
location: https://synbiohub.org/public/igem/BBa_I51001/annotation1910720/range1910720/1
component: BBa_B0045

https://synbiohub.org/public/igem/BBa_I51001/annotation1910720/range1910720/1
type: Range
persistent identity: range1910720
display id: range1910720
version: 1
direction: direction/reverse
top level: BBa_I51001
start: 1126
end: 1131
orientation: inline

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation1910698
display id: annotation1910698
version: 1
top level: BBa_I51001
location: https://synbiohub.org/public/igem/BBa_I51001/annotation1910698/range1910698/1
component: BBa_B0044

https://synbiohub.org/public/igem/BBa_I51001/annotation1910698/range1910698/1
type: Range
persistent identity: range1910698
display id: range1910698
version: 1
direction: direction/reverse
top level: BBa_I51001
start: 22
end: 34
orientation: inline

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation1910709
display id: annotation1910709
version: 1
top level: BBa_I51001
location: https://synbiohub.org/public/igem/BBa_I51001/annotation1910709/range1910709/1
component: BBa_G00102

https://synbiohub.org/public/igem/BBa_I51001/annotation1910709/range1910709/1
type: Range
persistent identity: range1910709
display id: range1910709
version: 1
direction: direction/reverse
top level: BBa_I51001
start: 116
end: 135
orientation: inline

BBa_K1404006
type: ComponentDefinition
persistent identity: BBa K1404006
display id: BBa_K1404006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: p70-CsgA, curli generator
creator: Juliette Paillet, Alexandre Duprey, Emiel van der Kouwe
created: 2014-09-23T11:00:00Z
modified: 2015-05-08T01:10:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1782_
m user id: 0
owner id: 21195
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: temp
mutable notes: temp
mutable provenance: temp
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: RBS Paris 2009
sequence annotation: CsgG signal
sequence annotation: Sec signal
sequence annotation: Beta sheet5
sequence annotation: mutation
sequence annotation: Beta sheet2
sequence annotation: Beta sheet1
sequence annotation: CsgA-WT
sequence annotation: Beta sheet3
sequence annotation: Beta sheet4
sequence annotation: Part:BBa_K342000
sequence: https://synbiohub.org/public/igem/BBa_K1404006_sequence/1

RBS Paris 2009
type: SequenceAnnotation
persistent identity: annotation2425875
display id: annotation2425875
version: 1
top level: BBa_K1404006
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425875/range2425875/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425875/range2425875/1
type: Range
persistent identity: range2425875
display id: range2425875
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 79
end: 91
orientation: inline

CsgG signal
type: SequenceAnnotation
persistent identity: annotation2425825
display id: annotation2425825
version: 1
top level: BBa_K1404006
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425825/range2425825/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425825/range2425825/1
type: Range
persistent identity: range2425825
display id: range2425825
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 152
end: 217
orientation: inline

Sec signal
type: SequenceAnnotation
persistent identity: annotation2425823
display id: annotation2425823
version: 1
top level: BBa_K1404006
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425823/range2425823/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425823/range2425823/1
type: Range
persistent identity: range2425823
display id: range2425823
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 92
end: 151
orientation: inline

Beta sheet5
type: SequenceAnnotation
persistent identity: annotation2425863
display id: annotation2425863
version: 1
top level: BBa_K1404006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425863/range2425863/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425863/range2425863/1
type: Range
persistent identity: range2425863
display id: range2425863
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 488
end: 544
orientation: inline

mutation
type: SequenceAnnotation
persistent identity: annotation2425844
display id: annotation2425844
version: 1
top level: BBa_K1404006
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425844/range2425844/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425844/range2425844/1
type: Range
persistent identity: range2425844
display id: range2425844
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 461
end: 463
orientation: inline

Beta sheet2
type: SequenceAnnotation
persistent identity: annotation2425860
display id: annotation2425860
version: 1
top level: BBa_K1404006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425860/range2425860/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425860/range2425860/1
type: Range
persistent identity: range2425860
display id: range2425860
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 287
end: 352
orientation: inline

Beta sheet1
type: SequenceAnnotation
persistent identity: annotation2425859
display id: annotation2425859
version: 1
top level: BBa_K1404006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425859/range2425859/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425859/range2425859/1
type: Range
persistent identity: range2425859
display id: range2425859
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 218
end: 286
orientation: inline

CsgA-WT
type: SequenceAnnotation
persistent identity: annotation2425824
display id: annotation2425824
version: 1
top level: BBa_K1404006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425824/range2425824/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425824/range2425824/1
type: Range
persistent identity: range2425824
display id: range2425824
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 92
end: 547
orientation: inline

Beta sheet3
type: SequenceAnnotation
persistent identity: annotation2425861
display id: annotation2425861
version: 1
top level: BBa_K1404006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425861/range2425861/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425861/range2425861/1
type: Range
persistent identity: range2425861
display id: range2425861
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 353
end: 421
orientation: inline

Beta sheet4
type: SequenceAnnotation
persistent identity: annotation2425862
display id: annotation2425862
version: 1
top level: BBa_K1404006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425862/range2425862/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425862/range2425862/1
type: Range
persistent identity: range2425862
display id: range2425862
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 422
end: 487
orientation: inline

Part:BBa_K342000
type: SequenceAnnotation
persistent identity: annotation2425822
display id: annotation2425822
version: 1
top level: BBa_K1404006
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425822/range2425822/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425822/range2425822/1
type: Range
persistent identity: range2425822
display id: range2425822
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 1
end: 70
orientation: inline

BB base ve
type: ComponentDefinition
persistent identity: BBa I51020
display id: BBa_I51020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BioBrick base vector (for constructing pSB**5 vectors)
creator: Reshma Shetty
created: 2008-01-05T12:00:00Z
modified: 2015-08-31T04:07:42Z
discontinued: true
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: This is a vector scaffold for constructing new BioBricks vectors. Information on the design is available at http://openwetware.org/wiki/Synthetic_Biology:Vectors/pSB%2A%2A5_design
mutable notes: Documented at http://openwetware.org/wiki/Synthetic_Biology:Vectors/pSB%2A%2A5_design Due to technical difficulties during synthesis, rather than including BBa_P1011 as designed, BBa_P1016 was synthesized as a component of this part. Hence, BBa_I51000 documents the original design and BBa_I51001 is the vector scaffold as synthesized. BBa_I52002 is the base vector as corrected from the synthesized construct.
mutable provenance: This is a composite part generated from several basic parts obtained from a variety of sources.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
component: BBa_I50022
component: BBa_B0053
component: BBa_B0062
component: BBa_G00001
component: BBa_B0043
component: BBa_B0042
component: BBa_B0055
component: BBa_B0045
component: BBa_G00102
component: BBa_B0054
component: BBa_B0044
component: BBa_G00000
component: BBa_P1016
component: BBa_G00100
component: BBa_P1006
sequence annotation: BBa_G00100
sequence annotation: BBa_P1016
sequence annotation: BBa_I50022
sequence annotation: BBa_P1006
sequence annotation: BBa_B0045
sequence annotation: BBa_B0043
sequence annotation: BBa_B0054
sequence annotation: BBa_G00001
sequence annotation: BBa_B0044
sequence annotation: BBa_B0062
sequence annotation: BBa_B0053
sequence annotation: BBa_G00102
sequence annotation: BBa_B0042
sequence annotation: BBa_G00000
sequence annotation: BBa_B0055
sequence: https://synbiohub.org/public/igem/BBa_I51020_sequence/1

BBa_I50022
type: Component
persistent identity: component1959277
display id: component1959277
version: 1
top level: BB base ve
access: public
definition: https://synbiohub.org/public/igem/BBa_I50022/1

BBa_B0053
type: Component
persistent identity: component1959242
display id: component1959242
version: 1
top level: BB base ve
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_B0062
type: Component
persistent identity: component1959231
display id: component1959231
version: 1
top level: BB base ve
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_G00001
type: Component
persistent identity: component1959213
display id: component1959213
version: 1
top level: BB base ve
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_B0043
type: Component
persistent identity: component1959259
display id: component1959259
version: 1
top level: BB base ve
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_B0042
type: Component
persistent identity: component1959254
display id: component1959254
version: 1
top level: BB base ve
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_B0055
type: Component
persistent identity: component1959247
display id: component1959247
version: 1
top level: BB base ve
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_B0045
type: Component
persistent identity: component1959240
display id: component1959240
version: 1
top level: BB base ve
access: public
definition: https://synbiohub.org/public/igem/BBa_B0045/1

BBa_G00102
type: Component
persistent identity: component1959229
display id: component1959229
version: 1
top level: BB base ve
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_B0045
type: Component
persistent identity: component1959233
display id: component1959233
version: 1
top level: BB base ve
access: public
definition: https://synbiohub.org/public/igem/BBa_B0045/1

BBa_B0054
type: Component
persistent identity: component1959228
display id: component1959228
version: 1
top level: BB base ve
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_B0042
type: Component
persistent identity: component1959225
display id: component1959225
version: 1
top level: BB base ve
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_B0044
type: Component
persistent identity: component1959218
display id: component1959218
version: 1
top level: BB base ve
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_G00000
type: Component
persistent identity: component1959263
display id: component1959263
version: 1
top level: BB base ve
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_P1016
type: Component
persistent identity: component1959267
display id: component1959267
version: 1
top level: BB base ve
access: public
definition: https://synbiohub.org/public/igem/BBa_P1016/1

BBa_G00100
type: Component
persistent identity: component1959244
display id: component1959244
version: 1
top level: BB base ve
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_P1006
type: Component
persistent identity: component1959238
display id: component1959238
version: 1
top level: BB base ve
access: public
definition: https://synbiohub.org/public/igem/BBa_P1006/1

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation1959244
display id: annotation1959244
version: 1
top level: BB base ve
location: https://synbiohub.org/public/igem/BBa_I51020/annotation1959244/range1959244/1
component: BBa_G00100

https://synbiohub.org/public/igem/BBa_I51020/annotation1959244/range1959244/1
type: Range
persistent identity: range1959244
display id: range1959244
version: 1
direction: direction/reverse
top level: BB base ve
start: 1204
end: 1223
orientation: inline

BBa_P1016
type: SequenceAnnotation
persistent identity: annotation1959267
display id: annotation1959267
version: 1
top level: BB base ve
location: https://synbiohub.org/public/igem/BBa_I51020/annotation1959267/range1959267/1
component: BBa_P1016

https://synbiohub.org/public/igem/BBa_I51020/annotation1959267/range1959267/1
type: Range
persistent identity: range1959267
display id: range1959267
version: 1
direction: direction/reverse
top level: BB base ve
start: 1349
end: 1764
orientation: inline

BBa_I50022
type: SequenceAnnotation
persistent identity: annotation1959277
display id: annotation1959277
version: 1
top level: BB base ve
location: https://synbiohub.org/public/igem/BBa_I51020/annotation1959277/range1959277/1
component: BBa_I50022

https://synbiohub.org/public/igem/BBa_I51020/annotation1959277/range1959277/1
type: Range
persistent identity: range1959277
display id: range1959277
version: 1
direction: direction/reverse
top level: BB base ve
start: 1765
end: 2438
orientation: inline

BBa_P1006
type: SequenceAnnotation
persistent identity: annotation1959238
display id: annotation1959238
version: 1
top level: BB base ve
location: https://synbiohub.org/public/igem/BBa_I51020/annotation1959238/range1959238/1
component: BBa_P1006

https://synbiohub.org/public/igem/BBa_I51020/annotation1959238/range1959238/1
type: Range
persistent identity: range1959238
display id: range1959238
version: 1
direction: direction/reverse
top level: BB base ve
start: 183
end: 1125
orientation: inline

BBa_B0045
type: SequenceAnnotation
persistent identity: annotation1959233
display id: annotation1959233
version: 1
top level: BB base ve
location: https://synbiohub.org/public/igem/BBa_I51020/annotation1959233/range1959233/1
component: BBa_B0045

https://synbiohub.org/public/igem/BBa_I51020/annotation1959233/range1959233/1
type: Range
persistent identity: range1959233
display id: range1959233
version: 1
direction: direction/reverse
top level: BB base ve
start: 177
end: 182
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation1959259
display id: annotation1959259
version: 1
top level: BB base ve
location: https://synbiohub.org/public/igem/BBa_I51020/annotation1959259/range1959259/1
component: BBa_B0043

https://synbiohub.org/public/igem/BBa_I51020/annotation1959259/range1959259/1
type: Range
persistent identity: range1959259
display id: range1959259
version: 1
direction: direction/reverse
top level: BB base ve
start: 1314
end: 1326
orientation: inline

BBa_B0045
type: SequenceAnnotation
persistent identity: annotation1959240
display id: annotation1959240
version: 1
top level: BB base ve
location: https://synbiohub.org/public/igem/BBa_I51020/annotation1959240/range1959240/1
component: BBa_B0045

https://synbiohub.org/public/igem/BBa_I51020/annotation1959240/range1959240/1
type: Range
persistent identity: range1959240
display id: range1959240
version: 1
direction: direction/reverse
top level: BB base ve
start: 1126
end: 1131
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation1959228
display id: annotation1959228
version: 1
top level: BB base ve
location: https://synbiohub.org/public/igem/BBa_I51020/annotation1959228/range1959228/1
component: BBa_B0054

https://synbiohub.org/public/igem/BBa_I51020/annotation1959228/range1959228/1
type: Range
persistent identity: range1959228
display id: range1959228
version: 1
direction: direction/reverse
top level: BB base ve
start: 47
end: 115
orientation: inline

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation1959213
display id: annotation1959213
version: 1
top level: BB base ve
location: https://synbiohub.org/public/igem/BBa_I51020/annotation1959213/range1959213/1
component: BBa_G00001

https://synbiohub.org/public/igem/BBa_I51020/annotation1959213/range1959213/1
type: Range
persistent identity: range1959213
display id: range1959213
version: 1
direction: direction/reverse
top level: BB base ve
start: 1
end: 21
orientation: inline

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation1959218
display id: annotation1959218
version: 1
top level: BB base ve
location: https://synbiohub.org/public/igem/BBa_I51020/annotation1959218/range1959218/1
component: BBa_B0044

https://synbiohub.org/public/igem/BBa_I51020/annotation1959218/range1959218/1
type: Range
persistent identity: range1959218
display id: range1959218
version: 1
direction: direction/reverse
top level: BB base ve
start: 22
end: 34
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation1959231
display id: annotation1959231
version: 1
top level: BB base ve
location: https://synbiohub.org/public/igem/BBa_I51020/annotation1959231/range1959231/1
component: BBa_B0062

https://synbiohub.org/public/igem/BBa_I51020/annotation1959231/range1959231/1
type: Range
persistent identity: range1959231
display id: range1959231
version: 1
direction: direction/reverse
top level: BB base ve
start: 136
end: 176
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation1959242
display id: annotation1959242
version: 1
top level: BB base ve
location: https://synbiohub.org/public/igem/BBa_I51020/annotation1959242/range1959242/1
component: BBa_B0053

https://synbiohub.org/public/igem/BBa_I51020/annotation1959242/range1959242/1
type: Range
persistent identity: range1959242
display id: range1959242
version: 1
direction: direction/reverse
top level: BB base ve
start: 1132
end: 1203
orientation: inline

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation1959229
display id: annotation1959229
version: 1
top level: BB base ve
location: https://synbiohub.org/public/igem/BBa_I51020/annotation1959229/range1959229/1
component: BBa_G00102

https://synbiohub.org/public/igem/BBa_I51020/annotation1959229/range1959229/1
type: Range
persistent identity: range1959229
display id: range1959229
version: 1
direction: direction/reverse
top level: BB base ve
start: 116
end: 135
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation1959254
display id: annotation1959254
version: 1
top level: BB base ve
location: https://synbiohub.org/public/igem/BBa_I51020/annotation1959254/range1959254/1
component: BBa_B0042

https://synbiohub.org/public/igem/BBa_I51020/annotation1959254/range1959254/1
type: Range
persistent identity: range1959254
display id: range1959254
version: 1
direction: direction/reverse
top level: BB base ve
start: 1302
end: 1313
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation1959263
display id: annotation1959263
version: 1
top level: BB base ve
location: https://synbiohub.org/public/igem/BBa_I51020/annotation1959263/range1959263/1
component: BBa_G00000

https://synbiohub.org/public/igem/BBa_I51020/annotation1959263/range1959263/1
type: Range
persistent identity: range1959263
display id: range1959263
version: 1
direction: direction/reverse
top level: BB base ve
start: 1327
end: 1348
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation1959247
display id: annotation1959247
version: 1
top level: BB base ve
location: https://synbiohub.org/public/igem/BBa_I51020/annotation1959247/range1959247/1
component: BBa_B0055

https://synbiohub.org/public/igem/BBa_I51020/annotation1959247/range1959247/1
type: Range
persistent identity: range1959247
display id: range1959247
version: 1
direction: direction/reverse
top level: BB base ve
start: 1224
end: 1301
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation1959225
display id: annotation1959225
version: 1
top level: BB base ve
location: https://synbiohub.org/public/igem/BBa_I51020/annotation1959225/range1959225/1
component: BBa_B0042

https://synbiohub.org/public/igem/BBa_I51020/annotation1959225/range1959225/1
type: Range
persistent identity: range1959225
display id: range1959225
version: 1
direction: direction/reverse
top level: BB base ve
start: 35
end: 46
orientation: inline

BBa_K319043
type: ComponentDefinition
persistent identity: BBa K319043
display id: BBa_K319043
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ADE4 targeting vector
creator: Afnan Azizi
created: 2010-10-25T11:00:00Z
modified: 2015-05-08T01:11:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _437_
m user id: 0
owner id: 7363
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is a targeting vector for homologous recombination into the ADE4 locus of S. cerevisiae. This vector can be used in cloning free cloning (ref. 2010 uOttawa iGEM wiki). Yeast strains with an ADE1 or ADE2 deletion or mutation have red colonies; by integrating the constructs using our protocol the ADE4 gene is knocked out and the colonies express a white phenotype.
mutable notes: N/A
mutable provenance: n
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: PstI/NsiI mixed site
sequence annotation: ADE4 terminator
sequence annotation: Cm resistance
sequence annotation: G5C5G5 filler site
sequence annotation: Forward landing pad
sequence annotation: Reverse landing pad
sequence annotation: EcoRI
sequence annotation: EcoRI/MfeI mixed site
sequence annotation: PstI site
sequence annotation: ATM1 terminator
sequence: https://synbiohub.org/public/igem/BBa_K319043_sequence/1

PstI/NsiI mixed site
type: SequenceAnnotation
persistent identity: annotation2109301
display id: annotation2109301
version: 1
top level: BBa_K319043
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K319043/annotation2109301/range2109301/1

https://synbiohub.org/public/igem/BBa_K319043/annotation2109301/range2109301/1
type: Range
persistent identity: range2109301
display id: range2109301
version: 1
direction: direction/forward
top level: BBa_K319043
start: 474
end: 479
orientation: inline

ADE4 terminator
type: SequenceAnnotation
persistent identity: annotation2109298
display id: annotation2109298
version: 1
top level: BBa_K319043
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K319043/annotation2109298/range2109298/1

https://synbiohub.org/public/igem/BBa_K319043/annotation2109298/range2109298/1
type: Range
persistent identity: range2109298
display id: range2109298
version: 1
direction: direction/forward
top level: BBa_K319043
start: 274
end: 473
orientation: inline

Cm resistance
type: SequenceAnnotation
persistent identity: annotation2109321
display id: annotation2109321
version: 1
top level: BBa_K319043
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K319043/annotation2109321/range2109321/1

https://synbiohub.org/public/igem/BBa_K319043/annotation2109321/range2109321/1
type: Range
persistent identity: range2109321
display id: range2109321
version: 1
direction: direction/reverse
top level: BBa_K319043
start: 1620
end: 2279
orientation: inline

G5C5G5 filler site
type: SequenceAnnotation
persistent identity: annotation2109294
display id: annotation2109294
version: 1
top level: BBa_K319043
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K319043/annotation2109294/range2109294/1

https://synbiohub.org/public/igem/BBa_K319043/annotation2109294/range2109294/1
type: Range
persistent identity: range2109294
display id: range2109294
version: 1
direction: direction/forward
top level: BBa_K319043
start: 233
end: 247
orientation: inline

Forward landing pad
type: SequenceAnnotation
persistent identity: annotation2109296
display id: annotation2109296
version: 1
top level: BBa_K319043
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K319043/annotation2109296/range2109296/1

https://synbiohub.org/public/igem/BBa_K319043/annotation2109296/range2109296/1
type: Range
persistent identity: range2109296
display id: range2109296
version: 1
direction: direction/forward
top level: BBa_K319043
start: 207
end: 226
orientation: inline

Reverse landing pad
type: SequenceAnnotation
persistent identity: annotation2109297
display id: annotation2109297
version: 1
top level: BBa_K319043
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K319043/annotation2109297/range2109297/1

https://synbiohub.org/public/igem/BBa_K319043/annotation2109297/range2109297/1
type: Range
persistent identity: range2109297
display id: range2109297
version: 1
direction: direction/reverse
top level: BBa_K319043
start: 254
end: 273
orientation: inline

EcoRI
type: SequenceAnnotation
persistent identity: annotation2109293
display id: annotation2109293
version: 1
top level: BBa_K319043
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K319043/annotation2109293/range2109293/1

https://synbiohub.org/public/igem/BBa_K319043/annotation2109293/range2109293/1
type: Range
persistent identity: range2109293
display id: range2109293
version: 1
direction: direction/forward
top level: BBa_K319043
start: 227
end: 232
orientation: inline

EcoRI/MfeI mixed site
type: SequenceAnnotation
persistent identity: annotation2109290
display id: annotation2109290
version: 1
top level: BBa_K319043
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K319043/annotation2109290/range2109290/1

https://synbiohub.org/public/igem/BBa_K319043/annotation2109290/range2109290/1
type: Range
persistent identity: range2109290
display id: range2109290
version: 1
direction: direction/forward
top level: BBa_K319043
start: 1
end: 6
orientation: inline

PstI site
type: SequenceAnnotation
persistent identity: annotation2109295
display id: annotation2109295
version: 1
top level: BBa_K319043
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K319043/annotation2109295/range2109295/1

https://synbiohub.org/public/igem/BBa_K319043/annotation2109295/range2109295/1
type: Range
persistent identity: range2109295
display id: range2109295
version: 1
direction: direction/forward
top level: BBa_K319043
start: 248
end: 253
orientation: inline

ATM1 terminator
type: SequenceAnnotation
persistent identity: annotation2109292
display id: annotation2109292
version: 1
top level: BBa_K319043
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K319043/annotation2109292/range2109292/1

https://synbiohub.org/public/igem/BBa_K319043/annotation2109292/range2109292/1
type: Range
persistent identity: range2109292
display id: range2109292
version: 1
direction: direction/forward
top level: BBa_K319043
start: 7
end: 206
orientation: inline

BBa_I51019
type: ComponentDefinition
persistent identity: BBa I51019
display id: BBa_I51019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BioBrick base vector, as synthesized by Codon Devices
creator: Reshma Shetty
created: 2008-03-22T12:00:00Z
modified: 2015-08-31T04:07:42Z
discontinued: true
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: BBa_I51019 is the sequence of the BioBrick base vector as synthesized by Codon Devices, Inc. in Cambridge, MA. The original design of the BioBrick base vector is available as BBa_I51000. BBa_I51001 is the sequence of the BioBrick base vector as reported by Codon Devices, Inc. although the true sequence is something like to BBa_I51019. The exact sequence of the provided DNA is difficult to determine. The corrected BioBrick base vector that was successfully constructed from BBa_I51019 is BBa_I51020.
mutable notes: Synthesis of the BioBrick base vector design (BBa_I51000) was problematic. We encountered two issues while trying to construct the base vector. First, troubleshooting efforts during synthesis compromised the design of our parts: failed attempts to clone the base vector into an ''E. coli'' strain intolerant of expression of the toxic protein CcdB led to an unnecessary redesign of the ''ccdB'' positive selection marker in the BioBrick base vector. As a result, a revised design for the BioBrick base vector (BBa_I51001) was specified instead. Second, faulty part design adversely impacted the synthesis process: our pUC19-based replication origin design (BBa_I50020) was similarly nonfunctional, so the revised base vector (BBa_I51001) could not be propagated as specified. We eventually determined that the provided DNA for the BioBrick base vector was actually a fusion of two slightly different copies of the base vector: one with a nonfunctional version of the pUC19 origin (BBa_I50020) and one with a functional version of the pUC19 origin (BBa_I50022). We corrected the synthesized BioBrick base vector (BBa_I51019) with molecular biology techniques to make the functional BioBrick base vector BBa_I51020 (see Methods). Commercial DNA synthesis processes currently rely on cloning, assembly and propagation of synthesized DNA in ''E. coli''. Difficulties during synthesis stemmed from the inclusion of both a ''ccdB'' positive selection marker that is toxic to most ''E. coli'' strains and a synthetic origin incapable of supporting plasmid replication of the BioBrick base vector. In general, for parts whose function are incompatible with growth and replication of ''E. coli'', the processes of DNA design and DNA synthesis cannot be easily decoupled.
mutable provenance: BBa_I51001
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
component: BBa_B0042
component: BBa_B0044
component: BBa_B0062
component: BBa_P1006
component: BBa_B0043
component: BBa_B0045
component: BBa_B0054
component: BBa_B0055
component: BBa_G00000
component: BBa_G00100
component: BBa_B0053
component: BBa_G00001
component: BBa_G00102
component: BBa_I50020
component: BBa_P1016
component: BBa_I50022
sequence annotation: BBa_B0062
sequence annotation: BBa_P1016
sequence annotation: BBa_B0055
sequence annotation: BBa_B0044
sequence annotation: BBa_G00100
sequence annotation: BBa_G00001
sequence annotation: BBa_G00102
sequence annotation: BBa_I50020
sequence annotation: BBa_B0054
sequence annotation: BBa_B0042
sequence annotation: BBa_B0053
sequence annotation: BBa_B0043

BBa_B0042
type: Component
persistent identity: component1961944
display id: component1961944
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_B0044
type: Component
persistent identity: component1961937
display id: component1961937
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_B0062
type: Component
persistent identity: component1961951
display id: component1961951
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_P1006
type: Component
persistent identity: component1961958
display id: component1961958
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_P1006/1

BBa_B0042
type: Component
persistent identity: component1961974
display id: component1961974
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_B0043
type: Component
persistent identity: component1961903
display id: component1961903
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_B0045
type: Component
persistent identity: component1961877
display id: component1961877
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0045/1

BBa_B0054
type: Component
persistent identity: component1961947
display id: component1961947
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_B0055
type: Component
persistent identity: component1961891
display id: component1961891
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_B0043
type: Component
persistent identity: component1961979
display id: component1961979
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_G00000
type: Component
persistent identity: component1961908
display id: component1961908
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_G00100
type: Component
persistent identity: component1961964
display id: component1961964
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_B0053
type: Component
persistent identity: component1961886
display id: component1961886
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_B0044
type: Component
persistent identity: component1961861
display id: component1961861
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_P1006
type: Component
persistent identity: component1961882
display id: component1961882
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_P1006/1

BBa_B0045
type: Component
persistent identity: component1961884
display id: component1961884
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0045/1

BBa_G00001
type: Component
persistent identity: component1961856
display id: component1961856
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_B0042
type: Component
persistent identity: component1961868
display id: component1961868
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_G00102
type: Component
persistent identity: component1961873
display id: component1961873
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_I50020
type: Component
persistent identity: component1961927
display id: component1961927
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_I50020/1

BBa_P1016
type: Component
persistent identity: component1961912
display id: component1961912
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_P1016/1

BBa_P1016
type: Component
persistent identity: component1961988
display id: component1961988
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_P1016/1

BBa_B0053
type: Component
persistent identity: component1961962
display id: component1961962
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_B0054
type: Component
persistent identity: component1961871
display id: component1961871
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_B0062
type: Component
persistent identity: component1961875
display id: component1961875
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_B0055
type: Component
persistent identity: component1961967
display id: component1961967
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_B0042
type: Component
persistent identity: component1961898
display id: component1961898
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_B0045
type: Component
persistent identity: component1961953
display id: component1961953
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0045/1

BBa_I50022
type: Component
persistent identity: component1961998
display id: component1961998
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_I50022/1

BBa_G00102
type: Component
persistent identity: component1961949
display id: component1961949
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_B0045
type: Component
persistent identity: component1961960
display id: component1961960
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0045/1

BBa_G00100
type: Component
persistent identity: component1961888
display id: component1961888
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_G00000
type: Component
persistent identity: component1961984
display id: component1961984
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_G00001
type: Component
persistent identity: component1961932
display id: component1961932
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation1961875
display id: annotation1961875
version: 1
top level: BBa_I51019
location: https://synbiohub.org/public/igem/BBa_I51019/annotation1961875/range1961875/1
component: BBa_B0062

https://synbiohub.org/public/igem/BBa_I51019/annotation1961875/range1961875/1
type: Range
persistent identity: range1961875
display id: range1961875
version: 1
direction: direction/reverse
top level: BBa_I51019
start: 136
end: 176
orientation: inline

BBa_P1016
type: SequenceAnnotation
persistent identity: annotation1961988
display id: annotation1961988
version: 1
top level: BBa_I51019
location: https://synbiohub.org/public/igem/BBa_I51019/annotation1961988/range1961988/1
component: BBa_P1016

https://synbiohub.org/public/igem/BBa_I51019/annotation1961988/range1961988/1
type: Range
persistent identity: range1961988
display id: range1961988
version: 1
direction: direction/reverse
top level: BBa_I51019
start: 3787
end: 4202
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation1961891
display id: annotation1961891
version: 1
top level: BBa_I51019
location: https://synbiohub.org/public/igem/BBa_I51019/annotation1961891/range1961891/1
component: BBa_B0055

https://synbiohub.org/public/igem/BBa_I51019/annotation1961891/range1961891/1
type: Range
persistent identity: range1961891
display id: range1961891
version: 1
direction: direction/reverse
top level: BBa_I51019
start: 1224
end: 1301
orientation: inline

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation1961937
display id: annotation1961937
version: 1
top level: BBa_I51019
location: https://synbiohub.org/public/igem/BBa_I51019/annotation1961937/range1961937/1
component: BBa_B0044

https://synbiohub.org/public/igem/BBa_I51019/annotation1961937/range1961937/1
type: Range
persistent identity: range1961937
display id: range1961937
version: 1
direction: direction/reverse
top level: BBa_I51019
start: 2460
end: 2472
orientation: inline

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation1961861
display id: annotation1961861
version: 1
top level: BBa_I51019
location: https://synbiohub.org/public/igem/BBa_I51019/annotation1961861/range1961861/1
component: BBa_B0044

https://synbiohub.org/public/igem/BBa_I51019/annotation1961861/range1961861/1
type: Range
persistent identity: range1961861
display id: range1961861
version: 1
direction: direction/reverse
top level: BBa_I51019
start: 22
end: 34
orientation: inline

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation1961888
display id: annotation1961888
version: 1
top level: BBa_I51019
location: https://synbiohub.org/public/igem/BBa_I51019/annotation1961888/range1961888/1
component: BBa_G00100

https://synbiohub.org/public/igem/BBa_I51019/annotation1961888/range1961888/1
type: Range
persistent identity: range1961888
display id: range1961888
version: 1
direction: direction/reverse
top level: BBa_I51019
start: 1204
end: 1223
orientation: inline

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation1961856
display id: annotation1961856
version: 1
top level: BBa_I51019
location: https://synbiohub.org/public/igem/BBa_I51019/annotation1961856/range1961856/1
component: BBa_G00001

https://synbiohub.org/public/igem/BBa_I51019/annotation1961856/range1961856/1
type: Range
persistent identity: range1961856
display id: range1961856
version: 1
direction: direction/reverse
top level: BBa_I51019
start: 1
end: 21
orientation: inline

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation1961949
display id: annotation1961949
version: 1
top level: BBa_I51019
location: https://synbiohub.org/public/igem/BBa_I51019/annotation1961949/range1961949/1
component: BBa_G00102

https://synbiohub.org/public/igem/BBa_I51019/annotation1961949/range1961949/1
type: Range
persistent identity: range1961949
display id: range1961949
version: 1
direction: direction/reverse
top level: BBa_I51019
start: 2554
end: 2573
orientation: inline

BBa_I50020
type: SequenceAnnotation
persistent identity: annotation1961927
display id: annotation1961927
version: 1
top level: BBa_I51019
location: https://synbiohub.org/public/igem/BBa_I51019/annotation1961927/range1961927/1
component: BBa_I50020

https://synbiohub.org/public/igem/BBa_I51019/annotation1961927/range1961927/1
type: Range
persistent identity: range1961927
display id: range1961927
version: 1
direction: direction/reverse
top level: BBa_I51019
start: 1765
end: 2438
orientation: inline

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation1961932
display id: annotation1961932
version: 1
top level: BBa_I51019
location: https://synbiohub.org/public/igem/BBa_I51019/annotation1961932/range1961932/1
component: BBa_G00001

https://synbiohub.org/public/igem/BBa_I51019/annotation1961932/range1961932/1
type: Range
persistent identity: range1961932
display id: range1961932
version: 1
direction: direction/reverse
top level: BBa_I51019
start: 2439
end: 2459
orientation: inline

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation1961964
display id: annotation1961964
version: 1
top level: BBa_I51019
location: https://synbiohub.org/public/igem/BBa_I51019/annotation1961964/range1961964/1
component: BBa_G00100

https://synbiohub.org/public/igem/BBa_I51019/annotation1961964/range1961964/1
type: Range
persistent identity: range1961964
display id: range1961964
version: 1
direction: direction/reverse
top level: BBa_I51019
start: 3642
end: 3661
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation1961947
display id: annotation1961947
version: 1
top level: BBa_I51019
location: https://synbiohub.org/public/igem/BBa_I51019/annotation1961947/range1961947/1
component: BBa_B0054

https://synbiohub.org/public/igem/BBa_I51019/annotation1961947/range1961947/1
type: Range
persistent identity: range1961947
display id: range1961947
version: 1
direction: direction/reverse
top level: BBa_I51019
start: 2485
end: 2553
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation1961868
display id: annotation1961868
version: 1
top level: BBa_I51019
location: https://synbiohub.org/public/igem/BBa_I51019/annotation1961868/range1961868/1
component: BBa_B0042

https://synbiohub.org/public/igem/BBa_I51019/annotation1961868/range1961868/1
type: Range
persistent identity: range1961868
display id: range1961868
version: 1
direction: direction/reverse
top level: BBa_I51019
start: 35
end: 46
orientation: inline

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation1961873
display id: annotation1961873
version: 1
top level: BBa_I51019
location: https://synbiohub.org/public/igem/BBa_I51019/annotation1961873/range1961873/1
component: BBa_G00102

https://synbiohub.org/public/igem/BBa_I51019/annotation1961873/range1961873/1
type: Range
persistent identity: range1961873
display id: range1961873
version: 1
direction: direction/reverse
top level: BBa_I51019
start: 116
end: 135
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation1961886
display id: annotation1961886
version: 1
top level: BBa_I51019
location: https://synbiohub.org/public/igem/BBa_I51019/annotation1961886/range1961886/1
component: BBa_B0053

https://synbiohub.org/public/igem/BBa_I51019/annotation1961886/range1961886/1
type: Range
persistent identity: range1961886
display id: range1961886
version: 1
direction: direction/reverse
top level: BBa_I51019
start: 1132
end: 1203
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation1961903
display id: annotation1961903
version: 1
top level: BBa_I51019
location: https://synbiohub.org/public/igem/BBa_I51019/annotation1961903/range1961903/1
component: BBa_B0043

https://synbiohub.org/public/igem/BBa_I51019/annotation1961903/range1961903/1
type: Range
persistent identity: range1961903
display id: range1961903
version: 1
direction: direction/reverse
top level: BBa_I51019
start: 1314
end: 1326
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation1961898
display id: annotation1961898
version: 1
top level: BBa_I51019
location: https://synbiohub.org/public/igem/BBa_I51019/annotation1961898/range1961898/1
component: BBa_B0042

https://synbiohub.org/public/igem/BBa_I51019/annotation1961898/range1961898/1
type: Range
persistent identity: range1961898
display id: range1961898
version: 1
direction: direction/reverse
top level: BBa_I51019
start: 1302
end: 1313
orientation: inline

BBa_I50022
type: SequenceAnnotation
persistent identity: annotation1961998
display id: annotation1961998
version: 1
top level: BBa_I51019
location: https://synbiohub.org/public/igem/BBa_I51019/annotation1961998/range1961998/1
component: BBa_I50022

https://synbiohub.org/public/igem/BBa_I51019/annotation1961998/range1961998/1
type: Range
persistent identity: range1961998
display id: range1961998
version: 1
direction: direction/reverse
top level: BBa_I51019
start: 4203
end: 4876
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation1961979
display id: annotation1961979
version: 1
top level: BBa_I51019
location: https://synbiohub.org/public/igem/BBa_I51019/annotation1961979/range1961979/1
component: BBa_B0043

https://synbiohub.org/public/igem/BBa_I51019/annotation1961979/range1961979/1
type: Range
persistent identity: range1961979
display id: range1961979
version: 1
direction: direction/reverse
top level: BBa_I51019
start: 3752
end: 3764
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation1961951
display id: annotation1961951
version: 1
top level: BBa_I51019
location: https://synbiohub.org/public/igem/BBa_I51019/annotation1961951/range1961951/1
component: BBa_B0062

https://synbiohub.org/public/igem/BBa_I51019/annotation1961951/range1961951/1
type: Range
persistent identity: range1961951
display id: range1961951
version: 1
direction: direction/reverse
top level: BBa_I51019
start: 2574
end: 2614
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation1961908
display id: annotation1961908
version: 1
top level: BBa_I51019
location: https://synbiohub.org/public/igem/BBa_I51019/annotation1961908/range1961908/1
component: BBa_G00000

https://synbiohub.org/public/igem/BBa_I51019/annotation1961908/range1961908/1
type: Range
persistent identity: range1961908
display id: range1961908
version: 1
direction: direction/reverse
top level: BBa_I51019
start: 1327
end: 1348
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation1961944
display id: annotation1961944
version: 1
top level: BBa_I51019
location: https://synbiohub.org/public/igem/BBa_I51019/annotation1961944/range1961944/1
component: BBa_B0042

https://synbiohub.org/public/igem/BBa_I51019/annotation1961944/range1961944/1
type: Range
persistent identity: range1961944
display id: range1961944
version: 1
direction: direction/reverse
top level: BBa_I51019
start: 2473
end: 2484
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation1961974
display id: annotation1961974
version: 1
top level: BBa_I51019
location: https://synbiohub.org/public/igem/BBa_I51019/annotation1961974/range1961974/1
component: BBa_B0042

https://synbiohub.org/public/igem/BBa_I51019/annotation1961974/range1961974/1
type: Range
persistent identity: range1961974
display id: range1961974
version: 1
direction: direction/reverse
top level: BBa_I51019
start: 3740
end: 3751
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation1961967
display id: annotation1961967
version: 1
top level: BBa_I51019
location: https://synbiohub.org/public/igem/BBa_I51019/annotation1961967/range1961967/1
component: BBa_B0055

https://synbiohub.org/public/igem/BBa_I51019/annotation1961967/range1961967/1
type: Range
persistent identity: range1961967
display id: range1961967
version: 1
direction: direction/reverse
top level: BBa_I51019
start: 3662
end: 3739
orientation: inline

BBa_B0045
type: SequenceAnnotation
persistent identity: annotation1961877
display id: annotation1961877
version: 1
top level: BBa_I51019
location: https://synbiohub.org/public/igem/BBa_I51019/annotation1961877/range1961877/1
component: BBa_B0045

https://synbiohub.org/public/igem/BBa_I51019/annotation1961877/range1961877/1
type: Range
persistent identity: range1961877
display id: range1961877
version: 1
direction: direction/reverse
top level: BBa_I51019
start: 177
end: 182
orientation: inline

BBa_B0045
type: SequenceAnnotation
persistent identity: annotation1961884
display id: annotation1961884
version: 1
top level: BBa_I51019
location: https://synbiohub.org/public/igem/BBa_I51019/annotation1961884/range1961884/1
component: BBa_B0045

https://synbiohub.org/public/igem/BBa_I51019/annotation1961884/range1961884/1
type: Range
persistent identity: range1961884
display id: range1961884
version: 1
direction: direction/reverse
top level: BBa_I51019
start: 1126
end: 1131
orientation: inline

BBa_P1016
type: SequenceAnnotation
persistent identity: annotation1961912
display id: annotation1961912
version: 1
top level: BBa_I51019
location: https://synbiohub.org/public/igem/BBa_I51019/annotation1961912/range1961912/1
component: BBa_P1016

https://synbiohub.org/public/igem/BBa_I51019/annotation1961912/range1961912/1
type: Range
persistent identity: range1961912
display id: range1961912
version: 1
direction: direction/reverse
top level: BBa_I51019
start: 1349
end: 1764
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation1961962
display id: annotation1961962
version: 1
top level: BBa_I51019
location: https://synbiohub.org/public/igem/BBa_I51019/annotation1961962/range1961962/1
component: BBa_B0053

https://synbiohub.org/public/igem/BBa_I51019/annotation1961962/range1961962/1
type: Range
persistent identity: range1961962
display id: range1961962
version: 1
direction: direction/reverse
top level: BBa_I51019
start: 3570
end: 3641
orientation: inline

BBa_P1006
type: SequenceAnnotation
persistent identity: annotation1961958
display id: annotation1961958
version: 1
top level: BBa_I51019
location: https://synbiohub.org/public/igem/BBa_I51019/annotation1961958/range1961958/1
component: BBa_P1006

https://synbiohub.org/public/igem/BBa_I51019/annotation1961958/range1961958/1
type: Range
persistent identity: range1961958
display id: range1961958
version: 1
direction: direction/reverse
top level: BBa_I51019
start: 2621
end: 3563
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation1961984
display id: annotation1961984
version: 1
top level: BBa_I51019
location: https://synbiohub.org/public/igem/BBa_I51019/annotation1961984/range1961984/1
component: BBa_G00000

https://synbiohub.org/public/igem/BBa_I51019/annotation1961984/range1961984/1
type: Range
persistent identity: range1961984
display id: range1961984
version: 1
direction: direction/reverse
top level: BBa_I51019
start: 3765
end: 3786
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation1961871
display id: annotation1961871
version: 1
top level: BBa_I51019
location: https://synbiohub.org/public/igem/BBa_I51019/annotation1961871/range1961871/1
component: BBa_B0054

https://synbiohub.org/public/igem/BBa_I51019/annotation1961871/range1961871/1
type: Range
persistent identity: range1961871
display id: range1961871
version: 1
direction: direction/reverse
top level: BBa_I51019
start: 47
end: 115
orientation: inline

BBa_P1006
type: SequenceAnnotation
persistent identity: annotation1961882
display id: annotation1961882
version: 1
top level: BBa_I51019
location: https://synbiohub.org/public/igem/BBa_I51019/annotation1961882/range1961882/1
component: BBa_P1006

https://synbiohub.org/public/igem/BBa_I51019/annotation1961882/range1961882/1
type: Range
persistent identity: range1961882
display id: range1961882
version: 1
direction: direction/reverse
top level: BBa_I51019
start: 183
end: 1125
orientation: inline

BBa_B0045
type: SequenceAnnotation
persistent identity: annotation1961953
display id: annotation1961953
version: 1
top level: BBa_I51019
location: https://synbiohub.org/public/igem/BBa_I51019/annotation1961953/range1961953/1
component: BBa_B0045

https://synbiohub.org/public/igem/BBa_I51019/annotation1961953/range1961953/1
type: Range
persistent identity: range1961953
display id: range1961953
version: 1
direction: direction/reverse
top level: BBa_I51019
start: 2615
end: 2620
orientation: inline

BBa_B0045
type: SequenceAnnotation
persistent identity: annotation1961960
display id: annotation1961960
version: 1
top level: BBa_I51019
location: https://synbiohub.org/public/igem/BBa_I51019/annotation1961960/range1961960/1
component: BBa_B0045

https://synbiohub.org/public/igem/BBa_I51019/annotation1961960/range1961960/1
type: Range
persistent identity: range1961960
display id: range1961960
version: 1
direction: direction/reverse
top level: BBa_I51019
start: 3564
end: 3569
orientation: inline

BBa_K394002
type: ComponentDefinition
persistent identity: BBa K394002
display id: BBa_K394002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plasmid for Chromosomal Integration in Yeast at Ura3
creator: Brian Landry
created: 2010-10-19T11:00:00Z
modified: 2015-05-08T01:12:21Z
discontinued: false
dominant: false
group u list: _504_
m user id: 0
owner id: 6327
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is designed to allow simple chromosomal integration of BioBrick plasmids. It is based off of the bacterial pSB1AT3 backbone allowing for normal DNA manipulation to occur in bacteria. However the BioBrick cut sites are flanked by homologous regions to the Ura3 locus in S. cerevisiae. To transform S. cerevisiae the plasmid is cut with BbsI creating two non-sticky ends. The linear fragment then contains Ura3 homologous sequences at its ends that can be used to chromosomally integrate the BioBrick plasmid into the yeast genome. Transformants can then be selected for using Ura- medium.
mutable notes: None
mutable provenance: This part was derived from plasmid pSB1AT3. Two successive rounds of PCR inserted a BbsI cut site and 40 bp homologous region in before the prefix and after the suffix. The following Primers were used: Forward 1 GCACAGAACAATAACCTGCTGGAAACGAAGATAAATCgaagacGATTACTTCGCGTTATGCAGGC Reverse 1 GCATCTTCTCAAATATGCTTCCCAGCCTGCTTATCcttctgAAATTCTGCCTCGTGATACGCC Forward 2 tactagtagcggccgctgcagTCTTAACCCAACTGCACAGAACAATAACCTGCTGGAAACG Reverse 2 ctctagaagcggccgcgaattcTTAGTATTGCTGGCCGCATCTTCTCAAATATGCTTCCCAGCC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: AmpR (rev)
sequence annotation: BbsI Cut Site
sequence annotation: ORI
sequence annotation: Ura3 homology
sequence annotation: VF2
sequence annotation: BioBrick Suffix
sequence annotation: rep (pMB1) (rev)
sequence annotation: VR
sequence annotation: BioBrick Prefix
sequence annotation: Tet-R
sequence: https://synbiohub.org/public/igem/BBa_K394002_sequence/1

AmpR (rev)
type: SequenceAnnotation
persistent identity: annotation2102742
display id: annotation2102742
version: 1
top level: BBa_K394002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K394002/annotation2102742/range2102742/1

https://synbiohub.org/public/igem/BBa_K394002/annotation2102742/range2102742/1
type: Range
persistent identity: range2102742
display id: range2102742
version: 1
direction: direction/reverse
top level: BBa_K394002
start: 2324
end: 3184
orientation: inline

BbsI Cut Site
type: SequenceAnnotation
persistent identity: annotation2103940
display id: annotation2103940
version: 1
top level: BBa_K394002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K394002/annotation2103940/range2103940/1

https://synbiohub.org/public/igem/BBa_K394002/annotation2103940/range2103940/1
type: Range
persistent identity: range2103940
display id: range2103940
version: 1
direction: direction/reverse
top level: BBa_K394002
start: 3381
end: 3386
orientation: inline

ORI
type: SequenceAnnotation
persistent identity: annotation2102740
display id: annotation2102740
version: 1
top level: BBa_K394002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K394002/annotation2102740/range2102740/1

https://synbiohub.org/public/igem/BBa_K394002/annotation2102740/range2102740/1
type: Range
persistent identity: range2102740
display id: range2102740
version: 1
direction: direction/forward
top level: BBa_K394002
start: 276
end: 276
orientation: inline

BbsI Cut Site
type: SequenceAnnotation
persistent identity: annotation2104024
display id: annotation2104024
version: 1
top level: BBa_K394002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K394002/annotation2104024/range2104024/1

https://synbiohub.org/public/igem/BBa_K394002/annotation2104024/range2104024/1
type: Range
persistent identity: range2104024
display id: range2104024
version: 1
direction: direction/forward
top level: BBa_K394002
start: 72
end: 77
orientation: inline

Ura3 homology
type: SequenceAnnotation
persistent identity: annotation2102715
display id: annotation2102715
version: 1
top level: BBa_K394002
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K394002/annotation2102715/range2102715/1

https://synbiohub.org/public/igem/BBa_K394002/annotation2102715/range2102715/1
type: Range
persistent identity: range2102715
display id: range2102715
version: 1
direction: direction/forward
top level: BBa_K394002
start: 22
end: 69
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2102743
display id: annotation2102743
version: 1
top level: BBa_K394002
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K394002/annotation2102743/range2102743/1

https://synbiohub.org/public/igem/BBa_K394002/annotation2102743/range2102743/1
type: Range
persistent identity: range2102743
display id: range2102743
version: 1
direction: direction/forward
top level: BBa_K394002
start: 3307
end: 3326
orientation: inline

BioBrick Suffix
type: SequenceAnnotation
persistent identity: annotation2102714
display id: annotation2102714
version: 1
top level: BBa_K394002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K394002/annotation2102714/range2102714/1

https://synbiohub.org/public/igem/BBa_K394002/annotation2102714/range2102714/1
type: Range
persistent identity: range2102714
display id: range2102714
version: 1
direction: direction/forward
top level: BBa_K394002
start: 1
end: 21
orientation: inline

rep (pMB1) (rev)
type: SequenceAnnotation
persistent identity: annotation2102717
display id: annotation2102717
version: 1
top level: BBa_K394002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K394002/annotation2102717/range2102717/1

https://synbiohub.org/public/igem/BBa_K394002/annotation2102717/range2102717/1
type: Range
persistent identity: range2102717
display id: range2102717
version: 1
direction: direction/reverse
top level: BBa_K394002
start: 261
end: 875
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2102716
display id: annotation2102716
version: 1
top level: BBa_K394002
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K394002/annotation2102716/range2102716/1

https://synbiohub.org/public/igem/BBa_K394002/annotation2102716/range2102716/1
type: Range
persistent identity: range2102716
display id: range2102716
version: 1
direction: direction/reverse
top level: BBa_K394002
start: 157
end: 176
orientation: inline

BioBrick Prefix
type: SequenceAnnotation
persistent identity: annotation2102745
display id: annotation2102745
version: 1
top level: BBa_K394002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K394002/annotation2102745/range2102745/1

https://synbiohub.org/public/igem/BBa_K394002/annotation2102745/range2102745/1
type: Range
persistent identity: range2102745
display id: range2102745
version: 1
direction: direction/forward
top level: BBa_K394002
start: 3435
end: 3457
orientation: inline

Ura3 homology
type: SequenceAnnotation
persistent identity: annotation2102744
display id: annotation2102744
version: 1
top level: BBa_K394002
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K394002/annotation2102744/range2102744/1

https://synbiohub.org/public/igem/BBa_K394002/annotation2102744/range2102744/1
type: Range
persistent identity: range2102744
display id: range2102744
version: 1
direction: direction/forward
top level: BBa_K394002
start: 3387
end: 3435
orientation: inline

Tet-R
type: SequenceAnnotation
persistent identity: annotation2102741
display id: annotation2102741
version: 1
top level: BBa_K394002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K394002/annotation2102741/range2102741/1

https://synbiohub.org/public/igem/BBa_K394002/annotation2102741/range2102741/1
type: Range
persistent identity: range2102741
display id: range2102741
version: 1
direction: direction/forward
top level: BBa_K394002
start: 1127
end: 2317
orientation: inline