//plasmid/chromosomalintegration
type: Collection
persistent identity: plasmid chromosomalintegration
display id: plasmid_chromosomalintegration
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: pSBBs2E
member: BBa_J179000
member: BBa_K394001
member: BBa_K510000
member: pSBBs4S
member: BBa_K319043
member: BBa_K950008
member: BBa_K382000
member: BBa_K510001
member: BBa_J179001
member: BBa_K382004
member: BBa_K863201
member: pSBBs1C
member: BBa_K1930007
member: BBa_K394002
member: p68
member: BBa_J179002
member: BBa_K382005
member: BBa_K382001
member: BBa_K090403
member: pSBBs3C
member: xyl+efe
member: BBa_K1976001
member: BBa_K1973006

BBa_K382004
type: ComponentDefinition
persistent identity: BBa K382004
display id: BBa_K382004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pORE Gus Reporter Series Vector with BiOBrick MCS (Kan resistance)
creator: Christina Agapakis
created: 2010-10-25T11:00:00Z
modified: 2015-06-11T01:18:58Z
discontinued: false
dominant: false
group u list: _488_
m user id: 4206
owner id: 2601
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Vector for transformation of plants. Cloning in E. coli, transfer to Agrobacterium, integrates into plant genome. Kan resistance marker in bacteria, confers Basta resistance in plants. Has a constitutive plant promoter and in-frame Gus reporter. Conforms to Silver BioBrick Assembly Standard 21.
mutable notes: Silver Standard 21.
mutable provenance: Modified vector pORE_R from the Arabidopsis Information Resource
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K382004_sequence/1

BBa_J179002
type: ComponentDefinition
persistent identity: BBa J179002
display id: BBa_J179002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBs4S: Bacillus subtilis vector, thrC integration, spec resistance
creator: Jara Radeck
created: 2013-12-20T12:00:00Z
modified: 2015-08-31T04:08:35Z
discontinued: false
dominant: false
group u list: _865_
m user id: 0
owner id: 6378
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is an empty backbone vector for the usage in Bacillus subtilis. It integrates in the thrC locus and can be selected with Spectinomycin cassette. It has a ampicillin resistance for cloning in E.coli. This backbone is a BioBricked version of the B. subtilis vector pDG1731.
mutable notes: This vector is compatible with pBS1C and pBS2E in one strain.
mutable provenance: This BioBrick is part of the LMU 2012 igem project BacillusBioBrickBox For handling of B. subtilis vectors, please see here. The transformation into B. subtilis is explained here.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: thrC,thrB back
sequence annotation: hom, trC front
sequence annotation: ori
sequence annotation: bla
sequence annotation: spc
sequence: https://synbiohub.org/public/igem/BBa_J179002_sequence/1

thrC,thrB back
type: SequenceAnnotation
persistent identity: annotation2371767
display id: annotation2371767
version: 1
top level: BBa_J179002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J179002/annotation2371767/range2371767/1

https://synbiohub.org/public/igem/BBa_J179002/annotation2371767/range2371767/1
type: Range
persistent identity: range2371767
display id: range2371767
version: 1
direction: direction/forward
top level: BBa_J179002
start: 1028
end: 1863
orientation: inline

hom, trC front
type: SequenceAnnotation
persistent identity: annotation2371770
display id: annotation2371770
version: 1
top level: BBa_J179002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J179002/annotation2371770/range2371770/1

https://synbiohub.org/public/igem/BBa_J179002/annotation2371770/range2371770/1
type: Range
persistent identity: range2371770
display id: range2371770
version: 1
direction: direction/forward
top level: BBa_J179002
start: 3731
end: 4542
orientation: inline

ori
type: SequenceAnnotation
persistent identity: annotation2371768
display id: annotation2371768
version: 1
top level: BBa_J179002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J179002/annotation2371768/range2371768/1

https://synbiohub.org/public/igem/BBa_J179002/annotation2371768/range2371768/1
type: Range
persistent identity: range2371768
display id: range2371768
version: 1
direction: direction/forward
top level: BBa_J179002
start: 1942
end: 2556
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2371769
display id: annotation2371769
version: 1
top level: BBa_J179002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J179002/annotation2371769/range2371769/1

https://synbiohub.org/public/igem/BBa_J179002/annotation2371769/range2371769/1
type: Range
persistent identity: range2371769
display id: range2371769
version: 1
direction: direction/reverse
top level: BBa_J179002
start: 2716
end: 3576
orientation: inline

spc
type: SequenceAnnotation
persistent identity: annotation2371766
display id: annotation2371766
version: 1
top level: BBa_J179002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J179002/annotation2371766/range2371766/1

https://synbiohub.org/public/igem/BBa_J179002/annotation2371766/range2371766/1
type: Range
persistent identity: range2371766
display id: range2371766
version: 1
direction: direction/forward
top level: BBa_J179002
start: 211
end: 885
orientation: inline

BBa_K510000
type: ComponentDefinition
persistent identity: BBa K510000
display id: BBa_K510000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pUC18Sfi-miniTn7BB-Gm
creator: David Caballero, Fernando Govantes
created: 2011-09-15T11:00:00Z
modified: 2015-05-08T01:12:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _673_
m user id: 0
owner id: 6157
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The miniTn7BB-Gm synthetic minitransposon was digested at the flanking SfiI sites and cloned into SfiI-digested pUC18-Sfi. This results in the elimination of the complete pUC18-SfiI multi-cloning site, except for the duplicated SfiI sites that remain on both sides of the transposon, thus facilitating its transfer to other vectors. This plasmid can be used to keep the mini-Tn7-Gm in E. coli and other enterics, and may be used for transposition into the genomes of non-enteric bacteria, in which it is non-replicative. The structure of the mini-Tn7BB-Gm transposon is shown in the figure below. Tn7R and Tn7L are the right and left ends of Tn7 transposon, respectively, required for recognition of the transposase machinery and transposition. FRT is the Flp recombinase target site for excision of the resistance once the transposon is inserted in the genome of a living organism. The gentamycin resistance cassette (Gm) is flanked by restriction sites in order to facilitate the generation of variants with different antibiotic resistance markers. The BioBrick cloning site (BCS) includes the prefix and suffix restrictions sites used in assembly standard 10, and it is the place where BioBricks are inserted for genome integration. The complete construction is flanked by SfiI restriction sites to facilitate cloning.
mutable notes: In order to make this vector compatible with assembly standard 10, several restriction sites were removed from the synthetic sequence.
mutable provenance: The pUC18-SfiI cloning vector was kindly provided by the Microbiology laboratory of Centro Andaluz de Biolog??a del Desarrollo (CABD) in Sevilla. The miniTn7BB-Gm synthetic transposon was designed by the iGEM 2011 team UPO-Sevilla based on the set of miniTn7 derivatives developed by Choi et al. (2005) and commercially synthesized by Mr. Gene (Regensburg, Germany). K.-H. Choi, J. B. Gaynor, K. G. White, C. Lopez, C. M. Bosio, R. R. Karkhoff-Schweizer & H. P. Schweizer (2005). A Tn7-based broad-range bacterial cloning and expression system. Nature Methods, vol.2 NO.6, June 2005, 443.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence annotation: ColEI (pUC18) Replication origin
sequence annotation: SfiI restriction site
sequence annotation: Ampicillin resistance (bla)
sequence annotation: Tn7-L end
sequence annotation: Suffix
sequence annotation: NcoI restriction site
sequence annotation: SphI restriction site
sequence annotation: Flipase recombination sequence (FRT)
sequence annotation: Tn7-R end
sequence annotation: E. coli his operon terminator
sequence annotation: Gentamycin resistance
sequence annotation: NcoI restrictino site
sequence annotation: Terminator
sequence annotation: Prefix
sequence annotation: VR (BBa_G00101) primer binding site
sequence annotation: VF2 (G00100) primer binding site
sequence annotation: double terminator (BBa_B0014)
sequence: https://synbiohub.org/public/igem/BBa_K510000_sequence/1

ColEI (pUC18) Replication origin
type: SequenceAnnotation
persistent identity: annotation2137417
display id: annotation2137417
version: 1
top level: BBa_K510000
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137417/range2137417/1

https://synbiohub.org/public/igem/BBa_K510000/annotation2137417/range2137417/1
type: Range
persistent identity: range2137417
display id: range2137417
version: 1
direction: direction/forward
top level: BBa_K510000
start: 1965
end: 2644
orientation: inline

SfiI restriction site
type: SequenceAnnotation
persistent identity: annotation2137415
display id: annotation2137415
version: 1
top level: BBa_K510000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137415/range2137415/1

https://synbiohub.org/public/igem/BBa_K510000/annotation2137415/range2137415/1
type: Range
persistent identity: range2137415
display id: range2137415
version: 1
direction: direction/forward
top level: BBa_K510000
start: 348
end: 360
orientation: inline

SfiI restriction site
type: SequenceAnnotation
persistent identity: annotation2137418
display id: annotation2137418
version: 1
top level: BBa_K510000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137418/range2137418/1

https://synbiohub.org/public/igem/BBa_K510000/annotation2137418/range2137418/1
type: Range
persistent identity: range2137418
display id: range2137418
version: 1
direction: direction/forward
top level: BBa_K510000
start: 3002
end: 3014
orientation: inline

Ampicillin resistance (bla)
type: SequenceAnnotation
persistent identity: annotation2137416
display id: annotation2137416
version: 1
top level: BBa_K510000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137416/range2137416/1

https://synbiohub.org/public/igem/BBa_K510000/annotation2137416/range2137416/1
type: Range
persistent identity: range2137416
display id: range2137416
version: 1
direction: direction/forward
top level: BBa_K510000
start: 966
end: 1826
orientation: inline

Tn7-L end
type: SequenceAnnotation
persistent identity: annotation2137414
display id: annotation2137414
version: 1
top level: BBa_K510000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137414/range2137414/1

https://synbiohub.org/public/igem/BBa_K510000/annotation2137414/range2137414/1
type: Range
persistent identity: range2137414
display id: range2137414
version: 1
direction: direction/forward
top level: BBa_K510000
start: 182
end: 347
orientation: inline

Suffix
type: SequenceAnnotation
persistent identity: annotation2137411
display id: annotation2137411
version: 1
top level: BBa_K510000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137411/range2137411/1

https://synbiohub.org/public/igem/BBa_K510000/annotation2137411/range2137411/1
type: Range
persistent identity: range2137411
display id: range2137411
version: 1
direction: direction/forward
top level: BBa_K510000
start: 2
end: 21
orientation: inline

NcoI restriction site
type: SequenceAnnotation
persistent identity: annotation2137439
display id: annotation2137439
version: 1
top level: BBa_K510000
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137439/range2137439/1

https://synbiohub.org/public/igem/BBa_K510000/annotation2137439/range2137439/1
type: Range
persistent identity: range2137439
display id: range2137439
version: 1
direction: direction/forward
top level: BBa_K510000
start: 4184
end: 4189
orientation: inline

SphI restriction site
type: SequenceAnnotation
persistent identity: annotation2137440
display id: annotation2137440
version: 1
top level: BBa_K510000
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137440/range2137440/1

https://synbiohub.org/public/igem/BBa_K510000/annotation2137440/range2137440/1
type: Range
persistent identity: range2137440
display id: range2137440
version: 1
direction: direction/forward
top level: BBa_K510000
start: 4190
end: 4195
orientation: inline

Flipase recombination sequence (FRT)
type: SequenceAnnotation
persistent identity: annotation2137422
display id: annotation2137422
version: 1
top level: BBa_K510000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137422/range2137422/1

https://synbiohub.org/public/igem/BBa_K510000/annotation2137422/range2137422/1
type: Range
persistent identity: range2137422
display id: range2137422
version: 1
direction: direction/forward
top level: BBa_K510000
start: 4196
end: 4243
orientation: inline

Tn7-R end
type: SequenceAnnotation
persistent identity: annotation2137419
display id: annotation2137419
version: 1
top level: BBa_K510000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137419/range2137419/1

https://synbiohub.org/public/igem/BBa_K510000/annotation2137419/range2137419/1
type: Range
persistent identity: range2137419
display id: range2137419
version: 1
direction: direction/forward
top level: BBa_K510000
start: 3014
end: 3213
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation2137412
display id: annotation2137412
version: 1
top level: BBa_K510000
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137412/range2137412/1

https://synbiohub.org/public/igem/BBa_K510000/annotation2137412/range2137412/1
type: Range
persistent identity: range2137412
display id: range2137412
version: 1
direction: direction/forward
top level: BBa_K510000
start: 22
end: 93
orientation: inline

SphI restriction site
type: SequenceAnnotation
persistent identity: annotation2137425
display id: annotation2137425
version: 1
top level: BBa_K510000
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137425/range2137425/1

https://synbiohub.org/public/igem/BBa_K510000/annotation2137425/range2137425/1
type: Range
persistent identity: range2137425
display id: range2137425
version: 1
direction: direction/forward
top level: BBa_K510000
start: 3381
end: 3386
orientation: inline

Gentamycin resistance
type: SequenceAnnotation
persistent identity: annotation2137421
display id: annotation2137421
version: 1
top level: BBa_K510000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137421/range2137421/1

https://synbiohub.org/public/igem/BBa_K510000/annotation2137421/range2137421/1
type: Range
persistent identity: range2137421
display id: range2137421
version: 1
direction: direction/reverse
top level: BBa_K510000
start: 3393
end: 3926
orientation: inline

NcoI restrictino site
type: SequenceAnnotation
persistent identity: annotation2137436
display id: annotation2137436
version: 1
top level: BBa_K510000
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137436/range2137436/1

https://synbiohub.org/public/igem/BBa_K510000/annotation2137436/range2137436/1
type: Range
persistent identity: range2137436
display id: range2137436
version: 1
direction: direction/forward
top level: BBa_K510000
start: 3387
end: 3392
orientation: inline

Flipase recombination sequence (FRT)
type: SequenceAnnotation
persistent identity: annotation2137420
display id: annotation2137420
version: 1
top level: BBa_K510000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137420/range2137420/1

https://synbiohub.org/public/igem/BBa_K510000/annotation2137420/range2137420/1
type: Range
persistent identity: range2137420
display id: range2137420
version: 1
direction: direction/forward
top level: BBa_K510000
start: 3329
end: 3376
orientation: inline

Terminator
type: SequenceAnnotation
persistent identity: annotation2137449
display id: annotation2137449
version: 1
top level: BBa_K510000
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137449/range2137449/1

https://synbiohub.org/public/igem/BBa_K510000/annotation2137449/range2137449/1
type: Range
persistent identity: range2137449
display id: range2137449
version: 1
direction: direction/forward
top level: BBa_K510000
start: 4326
end: 4359
orientation: inline

Prefix
type: SequenceAnnotation
persistent identity: annotation2137423
display id: annotation2137423
version: 1
top level: BBa_K510000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137423/range2137423/1

https://synbiohub.org/public/igem/BBa_K510000/annotation2137423/range2137423/1
type: Range
persistent identity: range2137423
display id: range2137423
version: 1
direction: direction/forward
top level: BBa_K510000
start: 4367
end: 4387
orientation: inline

VR (BBa_G00101) primer binding site
type: SequenceAnnotation
persistent identity: annotation2137413
display id: annotation2137413
version: 1
top level: BBa_K510000
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137413/range2137413/1

https://synbiohub.org/public/igem/BBa_K510000/annotation2137413/range2137413/1
type: Range
persistent identity: range2137413
display id: range2137413
version: 1
direction: direction/forward
top level: BBa_K510000
start: 157
end: 176
orientation: inline

VF2 (G00100) primer binding site
type: SequenceAnnotation
persistent identity: annotation2137442
display id: annotation2137442
version: 1
top level: BBa_K510000
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137442/range2137442/1

https://synbiohub.org/public/igem/BBa_K510000/annotation2137442/range2137442/1
type: Range
persistent identity: range2137442
display id: range2137442
version: 1
direction: direction/forward
top level: BBa_K510000
start: 4249
end: 4268
orientation: inline

double terminator (BBa_B0014)
type: SequenceAnnotation
persistent identity: annotation2137424
display id: annotation2137424
version: 1
top level: BBa_K510000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137424/range2137424/1

https://synbiohub.org/public/igem/BBa_K510000/annotation2137424/range2137424/1
type: Range
persistent identity: range2137424
display id: range2137424
version: 1
direction: direction/forward
top level: BBa_K510000
start: 3224
end: 3318
orientation: inline

BBa_J179000
type: ComponentDefinition
persistent identity: BBa J179000
display id: BBa_J179000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBS1C: Bacillus subtilis vector, amyE integration, CAM-resistance
creator: Jara Radeck
created: 2013-12-03T12:00:00Z
modified: 2015-08-31T04:08:35Z
discontinued: false
dominant: false
experience: experience/None
group u list: _865_
m user id: 0
owner id: 6378
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This vector integrates into the amyE-site of Bacillus subtilis via double recombination. The two recombination sites flank a standard BioBrick multiple cloning site (with RFP-cassette), as well as a chloramphenicol resistance cassette (5 ??g/ml). The backbone does not contain the restriction sites EcoRI, XbaI, SpeI, PstI, NgoMIV nor AgeI. The vector is replicative in Escherichia coli and provides resistance to ampicillin (100 ??g/ml). Before transformation into B. subtilis, this vector should be linearized with ScaI.
mutable notes: This is one of three empty vectors with compatible integration loci and resistance cassettes to allow combination of up to three constructs of choice in one single strain.
mutable provenance: pDG1731
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: amyE-front
sequence annotation: amyE-back
sequence annotation: cat
sequence annotation: ori
sequence annotation: bla
sequence: https://synbiohub.org/public/igem/BBa_J179000_sequence/1

amyE-front
type: SequenceAnnotation
persistent identity: annotation2371576
display id: annotation2371576
version: 1
top level: BBa_J179000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J179000/annotation2371576/range2371576/1

https://synbiohub.org/public/igem/BBa_J179000/annotation2371576/range2371576/1
type: Range
persistent identity: range2371576
display id: range2371576
version: 1
direction: direction/forward
top level: BBa_J179000
start: 5546
end: 6066
orientation: inline

amyE-back
type: SequenceAnnotation
persistent identity: annotation2371574
display id: annotation2371574
version: 1
top level: BBa_J179000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J179000/annotation2371574/range2371574/1

https://synbiohub.org/public/igem/BBa_J179000/annotation2371574/range2371574/1
type: Range
persistent identity: range2371574
display id: range2371574
version: 1
direction: direction/forward
top level: BBa_J179000
start: 1379
end: 2407
orientation: inline

cat
type: SequenceAnnotation
persistent identity: annotation2371573
display id: annotation2371573
version: 1
top level: BBa_J179000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J179000/annotation2371573/range2371573/1

https://synbiohub.org/public/igem/BBa_J179000/annotation2371573/range2371573/1
type: Range
persistent identity: range2371573
display id: range2371573
version: 1
direction: direction/forward
top level: BBa_J179000
start: 358
end: 1008
orientation: inline

ori
type: SequenceAnnotation
persistent identity: annotation2371577
display id: annotation2371577
version: 1
top level: BBa_J179000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J179000/annotation2371577/range2371577/1

https://synbiohub.org/public/igem/BBa_J179000/annotation2371577/range2371577/1
type: Range
persistent identity: range2371577
display id: range2371577
version: 1
direction: direction/forward
top level: BBa_J179000
start: 3587
end: 4201
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2371575
display id: annotation2371575
version: 1
top level: BBa_J179000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J179000/annotation2371575/range2371575/1

https://synbiohub.org/public/igem/BBa_J179000/annotation2371575/range2371575/1
type: Range
persistent identity: range2371575
display id: range2371575
version: 1
direction: direction/reverse
top level: BBa_J179000
start: 4361
end: 5221
orientation: inline

pSBBs1C
type: ComponentDefinition
persistent identity: BBa K823023
display id: BBa_K823023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSBBs1C: Empty backbone for integration into Bacillus subtilis amyE locus
creator: Jara Radeck
created: 2012-08-20T11:00:00Z
modified: 2015-05-08T01:13:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 0
owner id: 11555
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part is an empty backbone vector for the usage in Bacillus subtilis. It integrates in the amyE locus and can be selected with Chloramphenicol (cat gene). It has a ampicillin resistance for cloning in E.coli. It originates from the Bacillus vector pDG1662. This BioBrick is part of the LMU 2012 Bacillus BioBrick Box project: http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks
mutable notes: We removed any forbidden restriction sites by site-directed mutagenesis PCR.
mutable provenance: This backbone is an igem compatible version of the Bacillus vector pDG1662
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: bla
sequence annotation: amyE
sequence annotation: cat
sequence: https://synbiohub.org/public/igem/BBa_K823023_sequence/1

bla
type: SequenceAnnotation
persistent identity: annotation2180238
display id: annotation2180238
version: 1
top level: pSBBs1C
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823023/annotation2180238/range2180238/1

https://synbiohub.org/public/igem/BBa_K823023/annotation2180238/range2180238/1
type: Range
persistent identity: range2180238
display id: range2180238
version: 1
direction: direction/reverse
top level: pSBBs1C
start: 4361
end: 5221
orientation: inline

amyE
type: SequenceAnnotation
persistent identity: annotation2180239
display id: annotation2180239
version: 1
top level: pSBBs1C
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823023/annotation2180239/range2180239/1

https://synbiohub.org/public/igem/BBa_K823023/annotation2180239/range2180239/1
type: Range
persistent identity: range2180239
display id: range2180239
version: 1
direction: direction/forward
top level: pSBBs1C
start: 5546
end: 6066
orientation: inline

cat
type: SequenceAnnotation
persistent identity: annotation2180236
display id: annotation2180236
version: 1
top level: pSBBs1C
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823023/annotation2180236/range2180236/1

https://synbiohub.org/public/igem/BBa_K823023/annotation2180236/range2180236/1
type: Range
persistent identity: range2180236
display id: range2180236
version: 1
direction: direction/forward
top level: pSBBs1C
start: 358
end: 1008
orientation: inline

amyE
type: SequenceAnnotation
persistent identity: annotation2180237
display id: annotation2180237
version: 1
top level: pSBBs1C
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823023/annotation2180237/range2180237/1

https://synbiohub.org/public/igem/BBa_K823023/annotation2180237/range2180237/1
type: Range
persistent identity: range2180237
display id: range2180237
version: 1
direction: direction/forward
top level: pSBBs1C
start: 1379
end: 2407
orientation: inline

BBa_K1930007
type: ComponentDefinition
persistent identity: BBa K1930007
display id: BBa_K1930007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ciprofloxacin resistance cassette in integration plasmid (BBa_K823023)
creator: Barbora Waclawikova
created: 2016-10-15T11:00:00Z
modified: 2016-10-17T09:01:57Z
discontinued: true
dominant: false
experience: experience/Works
group u list: _2397_
m user id: 29748
owner id: 29748
owning group id: 9
status: status/Deleted
bookmark: false
mutable description: This part is a ciprofloxacin resistance cassette. It is containing a PAtpI constitutive promoter, ciprofloxacin resistance gene qnrS1 and double terminator. The promoter PAtpI ([http://parts.igem.org/Part:BBa_K1930005 ''BBa_K1930005'']) is a constitutive promoter which has its origin in Bacillus subtilis. It is responsible for the expression of atpA gene (ATP synthesis) during the first 30 minutes of the germination of B. subtilis. atpA gene is part of an operon (atpI-atpB-atpE-atpF-atpH-atpA-atpG-atpD-atpC), therefore the promoter region in front of the first protein coding gene (atpI) in this operon was chosen. Ciprofloxacin resistance gene qnrS1 is found naturally in E. coli, and other Gram-negative strains. qnr genes code for pentapeptide repeat proteins.??These proteins reduce susceptibility to quinolones by protecting the complex of DNA and DNA gyrase enzyme from the inhibitory effect of quinolones. Double terminator ([http://parts.igem.org/Part:BBa_B0015 ''BBa_B0015'']) is the most commonly used terminator. For more information see here: http://parts.igem.org/Part:BBa_B0015.
mutable notes: design
mutable provenance: Ciprofloxacin resistance cassette: Ordered as gBlock. IDT (Integrated DNA technologies). BBa_K823023: Created by iGEM Munich 2012. http://parts.igem.org/Part:BBa_K823023.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence annotation: BBa_B0015
sequence annotation: BBa_K1930005
sequence annotation: P(AtpI)
sequence annotation: QnrS1
sequence annotation: amyE
sequence annotation: cat
sequence annotation: bla
sequence annotation: 2TER
sequence annotation: BBa_K823023
sequence: https://synbiohub.org/public/igem/BBa_K1930007_sequence/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2514198
display id: annotation2514198
version: 1
top level: BBa_K1930007
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1930007/annotation2514198/range2514198/1

https://synbiohub.org/public/igem/BBa_K1930007/annotation2514198/range2514198/1
type: Range
persistent identity: range2514198
display id: range2514198
version: 1
direction: direction/forward
top level: BBa_K1930007
start: 1008
end: 1136
orientation: inline

BBa_K1930005
type: SequenceAnnotation
persistent identity: annotation2514181
display id: annotation2514181
version: 1
top level: BBa_K1930007
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1930007/annotation2514181/range2514181/1

https://synbiohub.org/public/igem/BBa_K1930007/annotation2514181/range2514181/1
type: Range
persistent identity: range2514181
display id: range2514181
version: 1
direction: direction/forward
top level: BBa_K1930007
start: 1
end: 350
orientation: inline

P(AtpI)
type: SequenceAnnotation
persistent identity: annotation2514180
display id: annotation2514180
version: 1
top level: BBa_K1930007
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1930007/annotation2514180/range2514180/1

https://synbiohub.org/public/igem/BBa_K1930007/annotation2514180/range2514180/1
type: Range
persistent identity: range2514180
display id: range2514180
version: 1
direction: direction/forward
top level: BBa_K1930007
start: 1
end: 350
orientation: inline

QnrS1
type: SequenceAnnotation
persistent identity: annotation2514182
display id: annotation2514182
version: 1
top level: BBa_K1930007
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1930007/annotation2514182/range2514182/1

https://synbiohub.org/public/igem/BBa_K1930007/annotation2514182/range2514182/1
type: Range
persistent identity: range2514182
display id: range2514182
version: 1
direction: direction/forward
top level: BBa_K1930007
start: 351
end: 1007
orientation: inline

amyE
type: SequenceAnnotation
persistent identity: annotation2514188
display id: annotation2514188
version: 1
top level: BBa_K1930007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1930007/annotation2514188/range2514188/1

https://synbiohub.org/public/igem/BBa_K1930007/annotation2514188/range2514188/1
type: Range
persistent identity: range2514188
display id: range2514188
version: 1
direction: direction/forward
top level: BBa_K1930007
start: 6661
end: 7181
orientation: inline

cat
type: SequenceAnnotation
persistent identity: annotation2514185
display id: annotation2514185
version: 1
top level: BBa_K1930007
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1930007/annotation2514185/range2514185/1

https://synbiohub.org/public/igem/BBa_K1930007/annotation2514185/range2514185/1
type: Range
persistent identity: range2514185
display id: range2514185
version: 1
direction: direction/forward
top level: BBa_K1930007
start: 1473
end: 2123
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2514187
display id: annotation2514187
version: 1
top level: BBa_K1930007
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1930007/annotation2514187/range2514187/1

https://synbiohub.org/public/igem/BBa_K1930007/annotation2514187/range2514187/1
type: Range
persistent identity: range2514187
display id: range2514187
version: 1
direction: direction/forward
top level: BBa_K1930007
start: 5476
end: 6336
orientation: inline

2TER
type: SequenceAnnotation
persistent identity: annotation2514183
display id: annotation2514183
version: 1
top level: BBa_K1930007
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1930007/annotation2514183/range2514183/1

https://synbiohub.org/public/igem/BBa_K1930007/annotation2514183/range2514183/1
type: Range
persistent identity: range2514183
display id: range2514183
version: 1
direction: direction/forward
top level: BBa_K1930007
start: 1008
end: 1136
orientation: inline

amyE
type: SequenceAnnotation
persistent identity: annotation2514186
display id: annotation2514186
version: 1
top level: BBa_K1930007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1930007/annotation2514186/range2514186/1

https://synbiohub.org/public/igem/BBa_K1930007/annotation2514186/range2514186/1
type: Range
persistent identity: range2514186
display id: range2514186
version: 1
direction: direction/forward
top level: BBa_K1930007
start: 2494
end: 3522
orientation: inline

BBa_K823023
type: SequenceAnnotation
persistent identity: annotation2514184
display id: annotation2514184
version: 1
top level: BBa_K1930007
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1930007/annotation2514184/range2514184/1

https://synbiohub.org/public/igem/BBa_K1930007/annotation2514184/range2514184/1
type: Range
persistent identity: range2514184
display id: range2514184
version: 1
direction: direction/forward
top level: BBa_K1930007
start: 1137
end: 7198
orientation: inline

BBa_J179001
type: ComponentDefinition
persistent identity: BBa J179001
display id: BBa_J179001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBS2E: Bacillus subtilis vector, lacA integration, MLS resistance
creator: Jara Radeck
created: 2013-12-20T12:00:00Z
modified: 2015-08-31T04:08:35Z
discontinued: false
dominant: false
experience: experience/None
group u list: _865_
m user id: 0
owner id: 6378
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is an empty backbone vector for the usage in Bacillus subtilis. It integrates in the lacA locus and can be selected with erythromycin + lincomycin (MLS). It has a ampicillin resistance for cloning in E.coli.
mutable notes: Compatible with pBs1C and pBs4S in one strain.
mutable provenance: This backbone is a BioBricked version of the B. subtilis vector pAX01. This BioBrick is part of the LMU 2012 igem project [http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks '''''Bacillus''B'''io'''B'''rick'''B'''ox] Reference: [http://www.ncbi.nlm.nih.gov/pubmed/11274134 H??rtl ''et al.'']
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: lacA front
sequence annotation: ori
sequence annotation: erm
sequence annotation: bla
sequence annotation: lacA back
sequence: https://synbiohub.org/public/igem/BBa_J179001_sequence/1

lacA front
type: SequenceAnnotation
persistent identity: annotation2371764
display id: annotation2371764
version: 1
top level: BBa_J179001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J179001/annotation2371764/range2371764/1

https://synbiohub.org/public/igem/BBa_J179001/annotation2371764/range2371764/1
type: Range
persistent identity: range2371764
display id: range2371764
version: 1
direction: direction/reverse
top level: BBa_J179001
start: 4078
end: 4593
orientation: inline

ori
type: SequenceAnnotation
persistent identity: annotation2371763
display id: annotation2371763
version: 1
top level: BBa_J179001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J179001/annotation2371763/range2371763/1

https://synbiohub.org/public/igem/BBa_J179001/annotation2371763/range2371763/1
type: Range
persistent identity: range2371763
display id: range2371763
version: 1
direction: direction/reverse
top level: BBa_J179001
start: 1758
end: 2381
orientation: inline

erm
type: SequenceAnnotation
persistent identity: annotation2371765
display id: annotation2371765
version: 1
top level: BBa_J179001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J179001/annotation2371765/range2371765/1

https://synbiohub.org/public/igem/BBa_J179001/annotation2371765/range2371765/1
type: Range
persistent identity: range2371765
display id: range2371765
version: 1
direction: direction/reverse
top level: BBa_J179001
start: 4891
end: 5628
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2371762
display id: annotation2371762
version: 1
top level: BBa_J179001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J179001/annotation2371762/range2371762/1

https://synbiohub.org/public/igem/BBa_J179001/annotation2371762/range2371762/1
type: Range
persistent identity: range2371762
display id: range2371762
version: 1
direction: direction/forward
top level: BBa_J179001
start: 747
end: 1607
orientation: inline

lacA back
type: SequenceAnnotation
persistent identity: annotation2371761
display id: annotation2371761
version: 1
top level: BBa_J179001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J179001/annotation2371761/range2371761/1

https://synbiohub.org/public/igem/BBa_J179001/annotation2371761/range2371761/1
type: Range
persistent identity: range2371761
display id: range2371761
version: 1
direction: direction/reverse
top level: BBa_J179001
start: 124
end: 531
orientation: inline

pSBBs1C
type: ComponentDefinition
persistent identity: BBa K823021
display id: BBa_K823021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSBBs1C-lacZ (lacZ reporter vector for B. subtilis)
creator: Jara Radeck
created: 2012-08-15T11:00:00Z
modified: 2015-05-08T01:13:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 0
owner id: 6378
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is a backbone vector for Bacillus subtilis. It integrates at the amyE locus. It has a Ampicillin resistance for cloning in E.coli and Chloramphenicol resistance for B.subtilis. The multiple cloning site is followed by a lacZ gene for reporter assays. This BioBrick is part of the LMU 2012 igem project Bacillus BioBrick Box http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks
mutable notes: This reporter vector can be used with the normal BioBrick assembly strategies.
mutable provenance: This vector is the Bacillus vector pAC6 brought into the iGEM BioBrick standard.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: lacZ
sequence annotation: bla
sequence annotation: cat
sequence annotation: amyE'
sequence annotation: 'amyE
sequence: https://synbiohub.org/public/igem/BBa_K823021_sequence/1

lacZ
type: SequenceAnnotation
persistent identity: annotation2179602
display id: annotation2179602
version: 1
top level: pSBBs1C
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823021/annotation2179602/range2179602/1

https://synbiohub.org/public/igem/BBa_K823021/annotation2179602/range2179602/1
type: Range
persistent identity: range2179602
display id: range2179602
version: 1
direction: direction/forward
top level: pSBBs1C
start: 49
end: 3112
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2179604
display id: annotation2179604
version: 1
top level: pSBBs1C
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823021/annotation2179604/range2179604/1

https://synbiohub.org/public/igem/BBa_K823021/annotation2179604/range2179604/1
type: Range
persistent identity: range2179604
display id: range2179604
version: 1
direction: direction/forward
top level: pSBBs1C
start: 4023
end: 4883
orientation: inline

cat
type: SequenceAnnotation
persistent identity: annotation2179606
display id: annotation2179606
version: 1
top level: pSBBs1C
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823021/annotation2179606/range2179606/1

https://synbiohub.org/public/igem/BBa_K823021/annotation2179606/range2179606/1
type: Range
persistent identity: range2179606
display id: range2179606
version: 1
direction: direction/reverse
top level: pSBBs1C
start: 8813
end: 9463
orientation: inline

amyE'
type: SequenceAnnotation
persistent identity: annotation2179603
display id: annotation2179603
version: 1
top level: pSBBs1C
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823021/annotation2179603/range2179603/1

https://synbiohub.org/public/igem/BBa_K823021/annotation2179603/range2179603/1
type: Range
persistent identity: range2179603
display id: range2179603
version: 1
direction: direction/reverse
top level: pSBBs1C
start: 3165
end: 3698
orientation: inline

'amyE
type: SequenceAnnotation
persistent identity: annotation2179605
display id: annotation2179605
version: 1
top level: pSBBs1C
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823021/annotation2179605/range2179605/1

https://synbiohub.org/public/igem/BBa_K823021/annotation2179605/range2179605/1
type: Range
persistent identity: range2179605
display id: range2179605
version: 1
direction: direction/reverse
top level: pSBBs1C
start: 7402
end: 8229
orientation: inline

BBa_K510001
type: ComponentDefinition
persistent identity: BBa K510001
display id: BBa_K510001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pUC18SfiI-miniTn7BB-Cm
creator: Fernando Govantes
created: 2011-09-15T11:00:00Z
modified: 2015-05-08T01:12:30Z
discontinued: false
dominant: false
group u list: _673_
m user id: 0
owner id: 6157
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The miniTn7BB-Cm minitransposon cloned into SfiI-digested pUC18-Sfi. This results in the elimination of the complete pUC18-SfiI multi-cloning site, except for the duplicated SfiI sites that remain on both sides of the transposon, thus facilitating its transfer to other vectors. This plasmid can be used to maintain the mini-Tn7-Cm in E. coli and other enterics, and may be used for transposition into the genomes of non-enteric bacteria, in which it is non-replicative. The structure of the mini-Tn7BB-Cm transposon is shown in the figure below. Tn7R and Tn7L are the right and left ends of Tn7 transposon, respectively, required for recognition of the transposase machinery and transposition. FRT is the Flp recombinase target site for excision of the resistance once the transposon is inserted in the genome of a living organism. The chloramphenicol resistance cassette (Cm) is flanked by restriction sites in order to facilitate the generation of variants with different antibiotic resistance markers. The BioBrick cloning site (BCS) includes the prefix and suffix restrictions sites used in assembly standard 10, and it is the place where BioBricks are inserted for genome integration. The complete construction is flanked by SfiI restriction sites to facilitate cloning.
mutable notes: NcoI and SphI sites were added at the ends of the chloramphenicol resistance cassette to facilitate cloning
mutable provenance: This construct is the result of replacing the gentamycin resistance cassette in pUC18SfiI-miniTn7BB-Gm by a chloramphenicol resistance cassette obtained from pBS1C3 by PCR amplification
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence annotation: Suffix
sequence annotation: Tn7-L end
sequence annotation: Prefix
sequence annotation: Flipase recombination sequence (FRT)
sequence annotation: ColEI (pUC18) Replication origin
sequence annotation: Chlormphenicol resistance (cat)
sequence annotation: Ampicillin resistance (bla)
sequence annotation: SfiI restriction site
sequence: https://synbiohub.org/public/igem/BBa_K510001_sequence/1

Suffix
type: SequenceAnnotation
persistent identity: annotation2129665
display id: annotation2129665
version: 1
top level: BBa_K510001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K510001/annotation2129665/range2129665/1

https://synbiohub.org/public/igem/BBa_K510001/annotation2129665/range2129665/1
type: Range
persistent identity: range2129665
display id: range2129665
version: 1
direction: direction/forward
top level: BBa_K510001
start: 2
end: 21
orientation: inline

Tn7-L end
type: SequenceAnnotation
persistent identity: annotation2129666
display id: annotation2129666
version: 1
top level: BBa_K510001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K510001/annotation2129666/range2129666/1

https://synbiohub.org/public/igem/BBa_K510001/annotation2129666/range2129666/1
type: Range
persistent identity: range2129666
display id: range2129666
version: 1
direction: direction/forward
top level: BBa_K510001
start: 82
end: 347
orientation: inline

Prefix
type: SequenceAnnotation
persistent identity: annotation2129682
display id: annotation2129682
version: 1
top level: BBa_K510001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K510001/annotation2129682/range2129682/1

https://synbiohub.org/public/igem/BBa_K510001/annotation2129682/range2129682/1
type: Range
persistent identity: range2129682
display id: range2129682
version: 1
direction: direction/forward
top level: BBa_K510001
start: 4479
end: 4499
orientation: inline

Flipase recombination sequence (FRT)
type: SequenceAnnotation
persistent identity: annotation2129680
display id: annotation2129680
version: 1
top level: BBa_K510001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K510001/annotation2129680/range2129680/1

https://synbiohub.org/public/igem/BBa_K510001/annotation2129680/range2129680/1
type: Range
persistent identity: range2129680
display id: range2129680
version: 1
direction: direction/forward
top level: BBa_K510001
start: 4308
end: 4355
orientation: inline

ColEI (pUC18) Replication origin
type: SequenceAnnotation
persistent identity: annotation2129670
display id: annotation2129670
version: 1
top level: BBa_K510001
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K510001/annotation2129670/range2129670/1

https://synbiohub.org/public/igem/BBa_K510001/annotation2129670/range2129670/1
type: Range
persistent identity: range2129670
display id: range2129670
version: 1
direction: direction/forward
top level: BBa_K510001
start: 1965
end: 2644
orientation: inline

Chlormphenicol resistance (cat)
type: SequenceAnnotation
persistent identity: annotation2129684
display id: annotation2129684
version: 1
top level: BBa_K510001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K510001/annotation2129684/range2129684/1

https://synbiohub.org/public/igem/BBa_K510001/annotation2129684/range2129684/1
type: Range
persistent identity: range2129684
display id: range2129684
version: 1
direction: direction/reverse
top level: BBa_K510001
start: 3534
end: 4194
orientation: inline

Ampicillin resistance (bla)
type: SequenceAnnotation
persistent identity: annotation2129669
display id: annotation2129669
version: 1
top level: BBa_K510001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K510001/annotation2129669/range2129669/1

https://synbiohub.org/public/igem/BBa_K510001/annotation2129669/range2129669/1
type: Range
persistent identity: range2129669
display id: range2129669
version: 1
direction: direction/forward
top level: BBa_K510001
start: 966
end: 1826
orientation: inline

SfiI restriction site
type: SequenceAnnotation
persistent identity: annotation2129668
display id: annotation2129668
version: 1
top level: BBa_K510001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K510001/annotation2129668/range2129668/1

https://synbiohub.org/public/igem/BBa_K510001/annotation2129668/range2129668/1
type: Range
persistent identity: range2129668
display id: range2129668
version: 1
direction: direction/forward
top level: BBa_K510001
start: 348
end: 360
orientation: inline

SfiI restriction site
type: SequenceAnnotation
persistent identity: annotation2129671
display id: annotation2129671
version: 1
top level: BBa_K510001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K510001/annotation2129671/range2129671/1

https://synbiohub.org/public/igem/BBa_K510001/annotation2129671/range2129671/1
type: Range
persistent identity: range2129671
display id: range2129671
version: 1
direction: direction/forward
top level: BBa_K510001
start: 3002
end: 3014
orientation: inline

Flipase recombination sequence (FRT)
type: SequenceAnnotation
persistent identity: annotation2129672
display id: annotation2129672
version: 1
top level: BBa_K510001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K510001/annotation2129672/range2129672/1

https://synbiohub.org/public/igem/BBa_K510001/annotation2129672/range2129672/1
type: Range
persistent identity: range2129672
display id: range2129672
version: 1
direction: direction/forward
top level: BBa_K510001
start: 3329
end: 3376
orientation: inline

BBa_K950008
type: ComponentDefinition
persistent identity: BBa K950008
display id: BBa_K950008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pRS313 shuttle vector
creator: Jan Rudolph
created: 2012-07-28T11:00:00Z
modified: 2015-05-08T01:13:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1218_
m user id: 0
owner id: 13191
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pRS313 yeast plasmid
mutable notes: contains restriction sites needed for assembly of BioBricks
mutable provenance: Jansen lab Tuebingen University
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence annotation: lacZ
sequence annotation: F1_ORI
sequence annotation: MCS
sequence annotation: SpeI
sequence annotation: XbaI
sequence annotation: CEN6
sequence annotation: bla
sequence annotation: PMB1 ORI
sequence annotation: T3_P
sequence annotation: T7_P
sequence annotation: HIS3
sequence annotation: ARSH4
sequence: https://synbiohub.org/public/igem/BBa_K950008_sequence/1

lacZ
type: SequenceAnnotation
persistent identity: annotation2179278
display id: annotation2179278
version: 1
top level: BBa_K950008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K950008/annotation2179278/range2179278/1

https://synbiohub.org/public/igem/BBa_K950008/annotation2179278/range2179278/1
type: Range
persistent identity: range2179278
display id: range2179278
version: 1
direction: direction/reverse
top level: BBa_K950008
start: 1880
end: 2217
orientation: inline

F1_ORI
type: SequenceAnnotation
persistent identity: annotation2179287
display id: annotation2179287
version: 1
top level: BBa_K950008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K950008/annotation2179287/range2179287/1

https://synbiohub.org/public/igem/BBa_K950008/annotation2179287/range2179287/1
type: Range
persistent identity: range2179287
display id: range2179287
version: 1
direction: direction/forward
top level: BBa_K950008
start: 1647
end: 1746
orientation: inline

MCS
type: SequenceAnnotation
persistent identity: annotation2179282
display id: annotation2179282
version: 1
top level: BBa_K950008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K950008/annotation2179282/range2179282/1

https://synbiohub.org/public/igem/BBa_K950008/annotation2179282/range2179282/1
type: Range
persistent identity: range2179282
display id: range2179282
version: 1
direction: direction/forward
top level: BBa_K950008
start: 2073
end: 2174
orientation: inline

SpeI
type: SequenceAnnotation
persistent identity: annotation2179280
display id: annotation2179280
version: 1
top level: BBa_K950008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K950008/annotation2179280/range2179280/1

https://synbiohub.org/public/igem/BBa_K950008/annotation2179280/range2179280/1
type: Range
persistent identity: range2179280
display id: range2179280
version: 1
direction: direction/forward
top level: BBa_K950008
start: 2103
end: 2108
orientation: inline

XbaI
type: SequenceAnnotation
persistent identity: annotation2179281
display id: annotation2179281
version: 1
top level: BBa_K950008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K950008/annotation2179281/range2179281/1

https://synbiohub.org/public/igem/BBa_K950008/annotation2179281/range2179281/1
type: Range
persistent identity: range2179281
display id: range2179281
version: 1
direction: direction/forward
top level: BBa_K950008
start: 2097
end: 2102
orientation: inline

CEN6
type: SequenceAnnotation
persistent identity: annotation2179283
display id: annotation2179283
version: 1
top level: BBa_K950008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K950008/annotation2179283/range2179283/1

https://synbiohub.org/public/igem/BBa_K950008/annotation2179283/range2179283/1
type: Range
persistent identity: range2179283
display id: range2179283
version: 1
direction: direction/forward
top level: BBa_K950008
start: 4395
end: 4511
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2179279
display id: annotation2179279
version: 1
top level: BBa_K950008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K950008/annotation2179279/range2179279/1

https://synbiohub.org/public/igem/BBa_K950008/annotation2179279/range2179279/1
type: Range
persistent identity: range2179279
display id: range2179279
version: 1
direction: direction/reverse
top level: BBa_K950008
start: 3393
end: 4253
orientation: inline

PMB1 ORI
type: SequenceAnnotation
persistent identity: annotation2179288
display id: annotation2179288
version: 1
top level: BBa_K950008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K950008/annotation2179288/range2179288/1

https://synbiohub.org/public/igem/BBa_K950008/annotation2179288/range2179288/1
type: Range
persistent identity: range2179288
display id: range2179288
version: 1
direction: direction/forward
top level: BBa_K950008
start: 2635
end: 2635
orientation: inline

T3_P
type: SequenceAnnotation
persistent identity: annotation2179286
display id: annotation2179286
version: 1
top level: BBa_K950008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K950008/annotation2179286/range2179286/1

https://synbiohub.org/public/igem/BBa_K950008/annotation2179286/range2179286/1
type: Range
persistent identity: range2179286
display id: range2179286
version: 1
direction: direction/reverse
top level: BBa_K950008
start: 2192
end: 2211
orientation: inline

T7_P
type: SequenceAnnotation
persistent identity: annotation2179285
display id: annotation2179285
version: 1
top level: BBa_K950008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K950008/annotation2179285/range2179285/1

https://synbiohub.org/public/igem/BBa_K950008/annotation2179285/range2179285/1
type: Range
persistent identity: range2179285
display id: range2179285
version: 1
direction: direction/forward
top level: BBa_K950008
start: 2046
end: 2065
orientation: inline

HIS3
type: SequenceAnnotation
persistent identity: annotation2179277
display id: annotation2179277
version: 1
top level: BBa_K950008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K950008/annotation2179277/range2179277/1

https://synbiohub.org/public/igem/BBa_K950008/annotation2179277/range2179277/1
type: Range
persistent identity: range2179277
display id: range2179277
version: 1
direction: direction/forward
top level: BBa_K950008
start: 503
end: 1162
orientation: inline

ARSH4
type: SequenceAnnotation
persistent identity: annotation2179284
display id: annotation2179284
version: 1
top level: BBa_K950008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K950008/annotation2179284/range2179284/1

https://synbiohub.org/public/igem/BBa_K950008/annotation2179284/range2179284/1
type: Range
persistent identity: range2179284
display id: range2179284
version: 1
direction: direction/forward
top level: BBa_K950008
start: 4512
end: 4885
orientation: inline

BBa_K394001
type: ComponentDefinition
persistent identity: BBa K394001
display id: BBa_K394001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plasmid for Chromosomal Integration in Yeast at His3
creator: Brian Landry
created: 2010-10-19T11:00:00Z
modified: 2015-05-08T01:12:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _504_
m user id: 0
owner id: 6327
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This part is designed to allow simple chromosomal integration of BioBrick plasmids. It is based off of the bacterial pSB1AT3 backbone allowing for normal DNA manipulation to occur in bacteria. However the BioBrick cut sites are flanked by homologous regions to the His3 locus in S. cerevisiae. To transform S. cerevisiae the plasmid is cut with BbsI creating two non-sticky ends. The linear fragment then contains His3 homologous sequences at its ends that can be used to chromosomally integrate the BioBrick plasmid into the yeast genome. Transformants can then be selected for using His- medium.
mutable notes: None
mutable provenance: This part was derived from plasmid pSB1AT3. Two successive rounds of PCR inserted a BbsI cut site and 40 bp homologous region in before the prefix and after the suffix. The following Primers were used: Forward 1 GCACAGAACAATAACCTGCTGGAAACGAAGATAAATCgaagacGATTACTTCGCGTTATGCAGGC Reverse 1 GCATCTTCTCAAATATGCTTCCCAGCCTGCTTATCcttctgAAATTCTGCCTCGTGATACGCC Forward 2 tactagtagcggccgctgcagTCTTAACCCAACTGCACAGAACAATAACCTGCTGGAAACG Reverse 2 ctctagaagcggccgcgaattcTTAGTATTGCTGGCCGCATCTTCTCAAATATGCTTCCCAGCC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: VR
sequence annotation: BbsI Cut Site
sequence annotation: BioBrick Suffix
sequence annotation: ORI
sequence annotation: AmpR (rev)
sequence annotation: Tet-R
sequence annotation: BioBrick Prefix
sequence annotation: His3 homology
sequence annotation: rep (pMB1) (rev)
sequence annotation: VF2
sequence: https://synbiohub.org/public/igem/BBa_K394001_sequence/1

VR
type: SequenceAnnotation
persistent identity: annotation2102390
display id: annotation2102390
version: 1
top level: BBa_K394001
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2102390/range2102390/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2102390/range2102390/1
type: Range
persistent identity: range2102390
display id: range2102390
version: 1
direction: direction/reverse
top level: BBa_K394001
start: 157
end: 176
orientation: inline

BbsI Cut Site
type: SequenceAnnotation
persistent identity: annotation2104028
display id: annotation2104028
version: 1
top level: BBa_K394001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2104028/range2104028/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2104028/range2104028/1
type: Range
persistent identity: range2104028
display id: range2104028
version: 1
direction: direction/reverse
top level: BBa_K394001
start: 3379
end: 3385
orientation: inline

BioBrick Suffix
type: SequenceAnnotation
persistent identity: annotation2090942
display id: annotation2090942
version: 1
top level: BBa_K394001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2090942/range2090942/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2090942/range2090942/1
type: Range
persistent identity: range2090942
display id: range2090942
version: 1
direction: direction/forward
top level: BBa_K394001
start: 1
end: 21
orientation: inline

ORI
type: SequenceAnnotation
persistent identity: annotation2102635
display id: annotation2102635
version: 1
top level: BBa_K394001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2102635/range2102635/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2102635/range2102635/1
type: Range
persistent identity: range2102635
display id: range2102635
version: 1
direction: direction/forward
top level: BBa_K394001
start: 276
end: 276
orientation: inline

AmpR (rev)
type: SequenceAnnotation
persistent identity: annotation2102638
display id: annotation2102638
version: 1
top level: BBa_K394001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2102638/range2102638/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2102638/range2102638/1
type: Range
persistent identity: range2102638
display id: range2102638
version: 1
direction: direction/reverse
top level: BBa_K394001
start: 2324
end: 3184
orientation: inline

Tet-R
type: SequenceAnnotation
persistent identity: annotation2102637
display id: annotation2102637
version: 1
top level: BBa_K394001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2102637/range2102637/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2102637/range2102637/1
type: Range
persistent identity: range2102637
display id: range2102637
version: 1
direction: direction/forward
top level: BBa_K394001
start: 1127
end: 2317
orientation: inline

BioBrick Prefix
type: SequenceAnnotation
persistent identity: annotation2102649
display id: annotation2102649
version: 1
top level: BBa_K394001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2102649/range2102649/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2102649/range2102649/1
type: Range
persistent identity: range2102649
display id: range2102649
version: 1
direction: direction/forward
top level: BBa_K394001
start: 3435
end: 3457
orientation: inline

His3 homology
type: SequenceAnnotation
persistent identity: annotation2102389
display id: annotation2102389
version: 1
top level: BBa_K394001
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2102389/range2102389/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2102389/range2102389/1
type: Range
persistent identity: range2102389
display id: range2102389
version: 1
direction: direction/forward
top level: BBa_K394001
start: 22
end: 69
orientation: inline

rep (pMB1) (rev)
type: SequenceAnnotation
persistent identity: annotation2102598
display id: annotation2102598
version: 1
top level: BBa_K394001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2102598/range2102598/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2102598/range2102598/1
type: Range
persistent identity: range2102598
display id: range2102598
version: 1
direction: direction/reverse
top level: BBa_K394001
start: 261
end: 875
orientation: inline

His3 homology
type: SequenceAnnotation
persistent identity: annotation2102645
display id: annotation2102645
version: 1
top level: BBa_K394001
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2102645/range2102645/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2102645/range2102645/1
type: Range
persistent identity: range2102645
display id: range2102645
version: 1
direction: direction/forward
top level: BBa_K394001
start: 3386
end: 3435
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2102640
display id: annotation2102640
version: 1
top level: BBa_K394001
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2102640/range2102640/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2102640/range2102640/1
type: Range
persistent identity: range2102640
display id: range2102640
version: 1
direction: direction/forward
top level: BBa_K394001
start: 3307
end: 3326
orientation: inline

BbsI Cut Site
type: SequenceAnnotation
persistent identity: annotation2104026
display id: annotation2104026
version: 1
top level: BBa_K394001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2104026/range2104026/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2104026/range2104026/1
type: Range
persistent identity: range2104026
display id: range2104026
version: 1
direction: direction/forward
top level: BBa_K394001
start: 71
end: 76
orientation: inline

xyl+efe
type: ComponentDefinition
persistent identity: BBa K1065204
display id: BBa_K1065204
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Efe+Bba_B0015 in BBa_K823024 (pXyl)
creator: Viola Valentini
created: 2013-08-31T11:00:00Z
modified: 2015-05-08T01:08:59Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1373_
m user id: 0
owner id: 15998
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: 2-oxoglutarate oxygenase/decarboxylase is an Ethylene Forming Enzyme (EFE) that catalyze Ethylene biosynthesis from 2-oxoglutarate. This part is in the pXyl vector that has been sent to the Igem UNITN 2013 team from the LMU Munich 2012 team. This part was cloned by the iGEM Trento 2013 team for the creation of an aerobically engineered pathway for the control of fruit ripening. Further information about this part and its characterization can be found in the iGEM Trento 2013 wiki.
mutable notes: ===Design Notes=== The coding sequence was taken from Pseudomonas syringae pv. phaseolicola PK2. We firstly optimized the codons for both B. subtilis and E. coli and then synthesized the gene with an RBS sequence upstream of the start codon. We decided to include in the CDS two restriction sites (NgoMIV and AgeI) in order to allow people to use the Freiburg assembly. GAATTCGCGGCCGCTTCTAGATAAGGAGGAACTACTATGGCCGGCACC... ...TCGACCGGTTAATACTAGTAGCGGCCGCTGCAG
mutable provenance: This enzyme was firstly purified from Pseudomonas Siringae pv. phaseolicola PK2, a 2-oxoglutarate-dependent ethylene producing bacterium [1]. The CDS has been optimized for both E.coli and B.subtilis. We decided to include an RBS sequence too.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1065002
component: BBa_K823024
sequence annotation: BBa_K1065002
sequence annotation: BBa_K823024
sequence: https://synbiohub.org/public/igem/BBa_K1065204_sequence/1

BBa_K1065002
type: Component
persistent identity: component2334259
display id: component2334259
version: 1
top level: xyl+efe
access: public
definition: https://synbiohub.org/public/igem/BBa_K1065002/1

BBa_K823024
type: Component
persistent identity: component2334267
display id: component2334267
version: 1
top level: xyl+efe
access: public
definition: pSBBs4S

BBa_K1065002
type: SequenceAnnotation
persistent identity: annotation2334259
display id: annotation2334259
version: 1
top level: xyl+efe
location: https://synbiohub.org/public/igem/BBa_K1065204/annotation2334259/range2334259/1
component: BBa_K1065002

https://synbiohub.org/public/igem/BBa_K1065204/annotation2334259/range2334259/1
type: Range
persistent identity: range2334259
display id: range2334259
version: 1
direction: direction/reverse
top level: xyl+efe
start: 1
end: 1215
orientation: inline

BBa_K823024
type: SequenceAnnotation
persistent identity: annotation2334267
display id: annotation2334267
version: 1
top level: xyl+efe
location: https://synbiohub.org/public/igem/BBa_K1065204/annotation2334267/range2334267/1
component: BBa_K823024

https://synbiohub.org/public/igem/BBa_K1065204/annotation2334267/range2334267/1
type: Range
persistent identity: range2334267
display id: range2334267
version: 1
direction: direction/reverse
top level: xyl+efe
start: 1224
end: 6017
orientation: inline

pSBBs2E
type: ComponentDefinition
persistent identity: BBa K823027
display id: BBa_K823027
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSBBs2E: Empty backbone for integration into Bacillus subtilis lacA locus
creator: Jara Radeck
created: 2012-08-20T11:00:00Z
modified: 2015-05-08T01:13:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 0
owner id: 11555
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is an empty backbone vector for the usage in Bacillus subtilis. It integrates in the lacA locus and can be selected with erythromycin + lincomycin (MLS). It has a ampicillin resistance for cloning in E.coli. This BioBrick is part of the LMU 2012 Bacillus BioBrick Box project: http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks
mutable notes: We removed any forbidden restriction sites by site-directed mutagenesis PCR. The clonging site also includes a rfp for selection in E.coli.
mutable provenance: This part is an igem compatible version of the Bacillus subtilis vector pAX01
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: bla
sequence annotation: 'lacA
sequence annotation: erm
sequence annotation: lacA'
sequence: https://synbiohub.org/public/igem/BBa_K823027_sequence/1

bla
type: SequenceAnnotation
persistent identity: annotation2180265
display id: annotation2180265
version: 1
top level: pSBBs2E
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823027/annotation2180265/range2180265/1

https://synbiohub.org/public/igem/BBa_K823027/annotation2180265/range2180265/1
type: Range
persistent identity: range2180265
display id: range2180265
version: 1
direction: direction/reverse
top level: pSBBs2E
start: 4603
end: 5400
orientation: inline

'lacA
type: SequenceAnnotation
persistent identity: annotation2180266
display id: annotation2180266
version: 1
top level: pSBBs2E
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823027/annotation2180266/range2180266/1

https://synbiohub.org/public/igem/BBa_K823027/annotation2180266/range2180266/1
type: Range
persistent identity: range2180266
display id: range2180266
version: 1
direction: direction/reverse
top level: pSBBs2E
start: 5600
end: 6100
orientation: inline

erm
type: SequenceAnnotation
persistent identity: annotation2180263
display id: annotation2180263
version: 1
top level: pSBBs2E
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823027/annotation2180263/range2180263/1

https://synbiohub.org/public/igem/BBa_K823027/annotation2180263/range2180263/1
type: Range
persistent identity: range2180263
display id: range2180263
version: 1
direction: direction/forward
top level: pSBBs2E
start: 500
end: 1300
orientation: inline

lacA'
type: SequenceAnnotation
persistent identity: annotation2180264
display id: annotation2180264
version: 1
top level: pSBBs2E
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823027/annotation2180264/range2180264/1

https://synbiohub.org/public/igem/BBa_K823027/annotation2180264/range2180264/1
type: Range
persistent identity: range2180264
display id: range2180264
version: 1
direction: direction/reverse
top level: pSBBs2E
start: 1600
end: 2100
orientation: inline

BBa_K382000
type: ComponentDefinition
persistent identity: BBa K382000
display id: BBa_K382000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pORE Open Series Vector with BioBrick MCS (Basta resistance)
creator: Christina Agapakis
created: 2010-10-25T11:00:00Z
modified: 2015-06-11T01:19:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _488_
m user id: 4206
owner id: 2601
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Vector for transformation of plants. Cloning in E. coli, transfer to Agrobacterium, integrates into plant genome. Kan resistance marker in bacteria, confers Basta resistance in plants. Has no promoter or reporter.
mutable notes: Conforms to Silver Assembly Standard 21
mutable provenance: Modified vector pORE_O1 from the Arabidopsis Information Resource
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence: https://synbiohub.org/public/igem/BBa_K382000_sequence/1

BBa_K382005
type: ComponentDefinition
persistent identity: BBa K382005
display id: BBa_K382005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pORE GFP Reporter Series Vector with BioBrick MCS (Kan resistance)
creator: Christina Agapakis
created: 2010-10-25T11:00:00Z
modified: 2015-06-11T01:18:36Z
discontinued: false
dominant: false
group u list: _488_
m user id: 4206
owner id: 2601
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Vector for transformation of plants. Cloning in E. coli, transfer to Agrobacterium, integrates into plant genome. Kan resistance marker in bacteria, confers Kan resistance in plants. Has constitutive plant promoter and in-frame GFP reporter. Conforms to Silver BIoBrick Assembly Standard 21.
mutable notes: Silver Standard 21
mutable provenance: Modified vector pORE_R2 from the Arabidopsis Information Resource
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K382005_sequence/1

BBa_K319043
type: ComponentDefinition
persistent identity: BBa K319043
display id: BBa_K319043
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ADE4 targeting vector
creator: Afnan Azizi
created: 2010-10-25T11:00:00Z
modified: 2015-05-08T01:11:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _437_
m user id: 0
owner id: 7363
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is a targeting vector for homologous recombination into the ADE4 locus of S. cerevisiae. This vector can be used in cloning free cloning (ref. 2010 uOttawa iGEM wiki). Yeast strains with an ADE1 or ADE2 deletion or mutation have red colonies; by integrating the constructs using our protocol the ADE4 gene is knocked out and the colonies express a white phenotype.
mutable notes: N/A
mutable provenance: n
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: ADE4 terminator
sequence annotation: PstI site
sequence annotation: G5C5G5 filler site
sequence annotation: PstI/NsiI mixed site
sequence annotation: Cm resistance
sequence annotation: EcoRI/MfeI mixed site
sequence annotation: EcoRI
sequence annotation: ATM1 terminator
sequence annotation: Reverse landing pad
sequence annotation: Forward landing pad
sequence: https://synbiohub.org/public/igem/BBa_K319043_sequence/1

ADE4 terminator
type: SequenceAnnotation
persistent identity: annotation2109298
display id: annotation2109298
version: 1
top level: BBa_K319043
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K319043/annotation2109298/range2109298/1

https://synbiohub.org/public/igem/BBa_K319043/annotation2109298/range2109298/1
type: Range
persistent identity: range2109298
display id: range2109298
version: 1
direction: direction/forward
top level: BBa_K319043
start: 274
end: 473
orientation: inline

PstI site
type: SequenceAnnotation
persistent identity: annotation2109295
display id: annotation2109295
version: 1
top level: BBa_K319043
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K319043/annotation2109295/range2109295/1

https://synbiohub.org/public/igem/BBa_K319043/annotation2109295/range2109295/1
type: Range
persistent identity: range2109295
display id: range2109295
version: 1
direction: direction/forward
top level: BBa_K319043
start: 248
end: 253
orientation: inline

G5C5G5 filler site
type: SequenceAnnotation
persistent identity: annotation2109294
display id: annotation2109294
version: 1
top level: BBa_K319043
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K319043/annotation2109294/range2109294/1

https://synbiohub.org/public/igem/BBa_K319043/annotation2109294/range2109294/1
type: Range
persistent identity: range2109294
display id: range2109294
version: 1
direction: direction/forward
top level: BBa_K319043
start: 233
end: 247
orientation: inline

PstI/NsiI mixed site
type: SequenceAnnotation
persistent identity: annotation2109301
display id: annotation2109301
version: 1
top level: BBa_K319043
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K319043/annotation2109301/range2109301/1

https://synbiohub.org/public/igem/BBa_K319043/annotation2109301/range2109301/1
type: Range
persistent identity: range2109301
display id: range2109301
version: 1
direction: direction/forward
top level: BBa_K319043
start: 474
end: 479
orientation: inline

Cm resistance
type: SequenceAnnotation
persistent identity: annotation2109321
display id: annotation2109321
version: 1
top level: BBa_K319043
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K319043/annotation2109321/range2109321/1

https://synbiohub.org/public/igem/BBa_K319043/annotation2109321/range2109321/1
type: Range
persistent identity: range2109321
display id: range2109321
version: 1
direction: direction/reverse
top level: BBa_K319043
start: 1620
end: 2279
orientation: inline

EcoRI/MfeI mixed site
type: SequenceAnnotation
persistent identity: annotation2109290
display id: annotation2109290
version: 1
top level: BBa_K319043
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K319043/annotation2109290/range2109290/1

https://synbiohub.org/public/igem/BBa_K319043/annotation2109290/range2109290/1
type: Range
persistent identity: range2109290
display id: range2109290
version: 1
direction: direction/forward
top level: BBa_K319043
start: 1
end: 6
orientation: inline

EcoRI
type: SequenceAnnotation
persistent identity: annotation2109293
display id: annotation2109293
version: 1
top level: BBa_K319043
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K319043/annotation2109293/range2109293/1

https://synbiohub.org/public/igem/BBa_K319043/annotation2109293/range2109293/1
type: Range
persistent identity: range2109293
display id: range2109293
version: 1
direction: direction/forward
top level: BBa_K319043
start: 227
end: 232
orientation: inline

ATM1 terminator
type: SequenceAnnotation
persistent identity: annotation2109292
display id: annotation2109292
version: 1
top level: BBa_K319043
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K319043/annotation2109292/range2109292/1

https://synbiohub.org/public/igem/BBa_K319043/annotation2109292/range2109292/1
type: Range
persistent identity: range2109292
display id: range2109292
version: 1
direction: direction/forward
top level: BBa_K319043
start: 7
end: 206
orientation: inline

Reverse landing pad
type: SequenceAnnotation
persistent identity: annotation2109297
display id: annotation2109297
version: 1
top level: BBa_K319043
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K319043/annotation2109297/range2109297/1

https://synbiohub.org/public/igem/BBa_K319043/annotation2109297/range2109297/1
type: Range
persistent identity: range2109297
display id: range2109297
version: 1
direction: direction/reverse
top level: BBa_K319043
start: 254
end: 273
orientation: inline

Forward landing pad
type: SequenceAnnotation
persistent identity: annotation2109296
display id: annotation2109296
version: 1
top level: BBa_K319043
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K319043/annotation2109296/range2109296/1

https://synbiohub.org/public/igem/BBa_K319043/annotation2109296/range2109296/1
type: Range
persistent identity: range2109296
display id: range2109296
version: 1
direction: direction/forward
top level: BBa_K319043
start: 207
end: 226
orientation: inline

BBa_K382001
type: ComponentDefinition
persistent identity: BBa K382001
display id: BBa_K382001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pORE Open Serices Vector with BioBrick MCS (Kan resistance)
creator: Christina Agapakis
created: 2010-10-25T11:00:00Z
modified: 2015-06-11T01:19:10Z
discontinued: false
dominant: false
group u list: _488_
m user id: 4206
owner id: 2601
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Vector for transformation of plants. Cloning in E. coli, transfer to Agrobacterium, integrates into plant genome. Kan resistance marker in bacteria, confers Kan resistance in plants. Has no promoter or reporter. Conforms to Silver BioBrick Assembly Standard 21.
mutable notes: Silver Assembly Standard 21
mutable provenance: Modified vector pORE_O1 from the Arabidopsis Information Resource
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K382001_sequence/1

pSBBs4S
type: ComponentDefinition
persistent identity: BBa K823024
display id: BBa_K823024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSBBs4S-Pxyl: Integrative expression vector for Bacillus subtilis</i
creator: Jara Radeck
created: 2012-08-20T11:00:00Z
modified: 2015-05-08T01:13:30Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1081_
m user id: 0
owner id: 11555
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is an expression vector for Bacillus subtilis. It integrates at the thrC locus. It has a ampicillin resistance for cloning in E.coli and chloramphenicol resistance for selection in B.subtilis. The multiple cloning site downstream of a Pxyl promoter, that is inducible with xylose. This BioBrick is part of the LMU 2012 igem project Bacillus BioBrick Box http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks
mutable notes: We removed any forbidden restriction sites by site-directed mutagenesis PCR. The clonging site also includes a rfp for selection for cloning in E.coli.
mutable provenance: This part is an igem compatible version of the Bacillus expression vector pXT
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: 'thrC
sequence annotation: thrB
sequence annotation: bla
sequence annotation: spc
sequence annotation: Pxly region
sequence annotation: thrC'
sequence annotation: hom
sequence: https://synbiohub.org/public/igem/BBa_K823024_sequence/1

'thrC
type: SequenceAnnotation
persistent identity: annotation2180241
display id: annotation2180241
version: 1
top level: pSBBs4S
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823024/annotation2180241/range2180241/1

https://synbiohub.org/public/igem/BBa_K823024/annotation2180241/range2180241/1
type: Range
persistent identity: range2180241
display id: range2180241
version: 1
direction: direction/forward
top level: pSBBs4S
start: 1028
end: 1456
orientation: inline

thrB
type: SequenceAnnotation
persistent identity: annotation2180242
display id: annotation2180242
version: 1
top level: pSBBs4S
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823024/annotation2180242/range2180242/1

https://synbiohub.org/public/igem/BBa_K823024/annotation2180242/range2180242/1
type: Range
persistent identity: range2180242
display id: range2180242
version: 1
direction: direction/forward
top level: pSBBs4S
start: 1453
end: 1863
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2180243
display id: annotation2180243
version: 1
top level: pSBBs4S
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823024/annotation2180243/range2180243/1

https://synbiohub.org/public/igem/BBa_K823024/annotation2180243/range2180243/1
type: Range
persistent identity: range2180243
display id: range2180243
version: 1
direction: direction/reverse
top level: pSBBs4S
start: 2716
end: 3576
orientation: inline

spc
type: SequenceAnnotation
persistent identity: annotation2180240
display id: annotation2180240
version: 1
top level: pSBBs4S
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823024/annotation2180240/range2180240/1

https://synbiohub.org/public/igem/BBa_K823024/annotation2180240/range2180240/1
type: Range
persistent identity: range2180240
display id: range2180240
version: 1
direction: direction/forward
top level: pSBBs4S
start: 211
end: 885
orientation: inline

Pxly region
type: SequenceAnnotation
persistent identity: annotation2180246
display id: annotation2180246
version: 1
top level: pSBBs4S
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823024/annotation2180246/range2180246/1

https://synbiohub.org/public/igem/BBa_K823024/annotation2180246/range2180246/1
type: Range
persistent identity: range2180246
display id: range2180246
version: 1
direction: direction/forward
top level: pSBBs4S
start: 4539
end: 4763
orientation: inline

thrC'
type: SequenceAnnotation
persistent identity: annotation2180245
display id: annotation2180245
version: 1
top level: pSBBs4S
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823024/annotation2180245/range2180245/1

https://synbiohub.org/public/igem/BBa_K823024/annotation2180245/range2180245/1
type: Range
persistent identity: range2180245
display id: range2180245
version: 1
direction: direction/forward
top level: pSBBs4S
start: 4033
end: 4572
orientation: inline

hom
type: SequenceAnnotation
persistent identity: annotation2180244
display id: annotation2180244
version: 1
top level: pSBBs4S
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823024/annotation2180244/range2180244/1

https://synbiohub.org/public/igem/BBa_K823024/annotation2180244/range2180244/1
type: Range
persistent identity: range2180244
display id: range2180244
version: 1
direction: direction/forward
top level: pSBBs4S
start: 3731
end: 4033
orientation: inline

BBa_K394002
type: ComponentDefinition
persistent identity: BBa K394002
display id: BBa_K394002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plasmid for Chromosomal Integration in Yeast at Ura3
creator: Brian Landry
created: 2010-10-19T11:00:00Z
modified: 2015-05-08T01:12:21Z
discontinued: false
dominant: false
group u list: _504_
m user id: 0
owner id: 6327
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is designed to allow simple chromosomal integration of BioBrick plasmids. It is based off of the bacterial pSB1AT3 backbone allowing for normal DNA manipulation to occur in bacteria. However the BioBrick cut sites are flanked by homologous regions to the Ura3 locus in S. cerevisiae. To transform S. cerevisiae the plasmid is cut with BbsI creating two non-sticky ends. The linear fragment then contains Ura3 homologous sequences at its ends that can be used to chromosomally integrate the BioBrick plasmid into the yeast genome. Transformants can then be selected for using Ura- medium.
mutable notes: None
mutable provenance: This part was derived from plasmid pSB1AT3. Two successive rounds of PCR inserted a BbsI cut site and 40 bp homologous region in before the prefix and after the suffix. The following Primers were used: Forward 1 GCACAGAACAATAACCTGCTGGAAACGAAGATAAATCgaagacGATTACTTCGCGTTATGCAGGC Reverse 1 GCATCTTCTCAAATATGCTTCCCAGCCTGCTTATCcttctgAAATTCTGCCTCGTGATACGCC Forward 2 tactagtagcggccgctgcagTCTTAACCCAACTGCACAGAACAATAACCTGCTGGAAACG Reverse 2 ctctagaagcggccgcgaattcTTAGTATTGCTGGCCGCATCTTCTCAAATATGCTTCCCAGCC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: ORI
sequence annotation: Ura3 homology
sequence annotation: AmpR (rev)
sequence annotation: BbsI Cut Site
sequence annotation: VF2
sequence annotation: BioBrick Prefix
sequence annotation: Tet-R
sequence annotation: VR
sequence annotation: rep (pMB1) (rev)
sequence annotation: BioBrick Suffix
sequence: https://synbiohub.org/public/igem/BBa_K394002_sequence/1

ORI
type: SequenceAnnotation
persistent identity: annotation2102740
display id: annotation2102740
version: 1
top level: BBa_K394002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K394002/annotation2102740/range2102740/1

https://synbiohub.org/public/igem/BBa_K394002/annotation2102740/range2102740/1
type: Range
persistent identity: range2102740
display id: range2102740
version: 1
direction: direction/forward
top level: BBa_K394002
start: 276
end: 276
orientation: inline

Ura3 homology
type: SequenceAnnotation
persistent identity: annotation2102744
display id: annotation2102744
version: 1
top level: BBa_K394002
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K394002/annotation2102744/range2102744/1

https://synbiohub.org/public/igem/BBa_K394002/annotation2102744/range2102744/1
type: Range
persistent identity: range2102744
display id: range2102744
version: 1
direction: direction/forward
top level: BBa_K394002
start: 3387
end: 3435
orientation: inline

AmpR (rev)
type: SequenceAnnotation
persistent identity: annotation2102742
display id: annotation2102742
version: 1
top level: BBa_K394002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K394002/annotation2102742/range2102742/1

https://synbiohub.org/public/igem/BBa_K394002/annotation2102742/range2102742/1
type: Range
persistent identity: range2102742
display id: range2102742
version: 1
direction: direction/reverse
top level: BBa_K394002
start: 2324
end: 3184
orientation: inline

Ura3 homology
type: SequenceAnnotation
persistent identity: annotation2102715
display id: annotation2102715
version: 1
top level: BBa_K394002
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K394002/annotation2102715/range2102715/1

https://synbiohub.org/public/igem/BBa_K394002/annotation2102715/range2102715/1
type: Range
persistent identity: range2102715
display id: range2102715
version: 1
direction: direction/forward
top level: BBa_K394002
start: 22
end: 69
orientation: inline

BbsI Cut Site
type: SequenceAnnotation
persistent identity: annotation2104024
display id: annotation2104024
version: 1
top level: BBa_K394002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K394002/annotation2104024/range2104024/1

https://synbiohub.org/public/igem/BBa_K394002/annotation2104024/range2104024/1
type: Range
persistent identity: range2104024
display id: range2104024
version: 1
direction: direction/forward
top level: BBa_K394002
start: 72
end: 77
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2102743
display id: annotation2102743
version: 1
top level: BBa_K394002
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K394002/annotation2102743/range2102743/1

https://synbiohub.org/public/igem/BBa_K394002/annotation2102743/range2102743/1
type: Range
persistent identity: range2102743
display id: range2102743
version: 1
direction: direction/forward
top level: BBa_K394002
start: 3307
end: 3326
orientation: inline

BioBrick Prefix
type: SequenceAnnotation
persistent identity: annotation2102745
display id: annotation2102745
version: 1
top level: BBa_K394002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K394002/annotation2102745/range2102745/1

https://synbiohub.org/public/igem/BBa_K394002/annotation2102745/range2102745/1
type: Range
persistent identity: range2102745
display id: range2102745
version: 1
direction: direction/forward
top level: BBa_K394002
start: 3435
end: 3457
orientation: inline

Tet-R
type: SequenceAnnotation
persistent identity: annotation2102741
display id: annotation2102741
version: 1
top level: BBa_K394002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K394002/annotation2102741/range2102741/1

https://synbiohub.org/public/igem/BBa_K394002/annotation2102741/range2102741/1
type: Range
persistent identity: range2102741
display id: range2102741
version: 1
direction: direction/forward
top level: BBa_K394002
start: 1127
end: 2317
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2102716
display id: annotation2102716
version: 1
top level: BBa_K394002
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K394002/annotation2102716/range2102716/1

https://synbiohub.org/public/igem/BBa_K394002/annotation2102716/range2102716/1
type: Range
persistent identity: range2102716
display id: range2102716
version: 1
direction: direction/reverse
top level: BBa_K394002
start: 157
end: 176
orientation: inline

BbsI Cut Site
type: SequenceAnnotation
persistent identity: annotation2103940
display id: annotation2103940
version: 1
top level: BBa_K394002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K394002/annotation2103940/range2103940/1

https://synbiohub.org/public/igem/BBa_K394002/annotation2103940/range2103940/1
type: Range
persistent identity: range2103940
display id: range2103940
version: 1
direction: direction/reverse
top level: BBa_K394002
start: 3381
end: 3386
orientation: inline

rep (pMB1) (rev)
type: SequenceAnnotation
persistent identity: annotation2102717
display id: annotation2102717
version: 1
top level: BBa_K394002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K394002/annotation2102717/range2102717/1

https://synbiohub.org/public/igem/BBa_K394002/annotation2102717/range2102717/1
type: Range
persistent identity: range2102717
display id: range2102717
version: 1
direction: direction/reverse
top level: BBa_K394002
start: 261
end: 875
orientation: inline

BioBrick Suffix
type: SequenceAnnotation
persistent identity: annotation2102714
display id: annotation2102714
version: 1
top level: BBa_K394002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K394002/annotation2102714/range2102714/1

https://synbiohub.org/public/igem/BBa_K394002/annotation2102714/range2102714/1
type: Range
persistent identity: range2102714
display id: range2102714
version: 1
direction: direction/forward
top level: BBa_K394002
start: 1
end: 21
orientation: inline

pSBBs3C
type: ComponentDefinition
persistent identity: BBa K823025
display id: BBa_K823025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSBBs3C-luxABCDE (lux reporter vector for B.subtilis)
creator: Jara Radeck
created: 2012-08-20T11:00:00Z
modified: 2015-05-08T01:13:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 0
owner id: 11555
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is a reporter vector for Bacillus subtilis. It integrates at the sacA locus of B.subtilis genome. It has a ampicillin resistance for cloning in E.coli and Chloramphenicol resistance for selection in B. subtilis. The multiple cloning site has a rfp for selection and is followed by the luxABCDE gene cluster for luminescence reporter assays. This BioBrick is part of the LMU-Munich 2012 igem project Bacillus BioBrickBOX http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks
mutable notes: We removed any forbidden restriction sites by site-directed mutagenesis PCR. The clonging site also includes a rfp for selection for cloning in E.coli.
mutable provenance: This vector is an igem compatible version of the pAH328 Bacillus reporter vecotr.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: cat
sequence annotation: luxA
sequence annotation: luxB
sequence annotation: sacA
sequence annotation: luxC
sequence annotation: luxE
sequence annotation: luxD
sequence annotation: bla
sequence: https://synbiohub.org/public/igem/BBa_K823025_sequence/1

cat
type: SequenceAnnotation
persistent identity: annotation2180255
display id: annotation2180255
version: 1
top level: pSBBs3C
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823025/annotation2180255/range2180255/1

https://synbiohub.org/public/igem/BBa_K823025/annotation2180255/range2180255/1
type: Range
persistent identity: range2180255
display id: range2180255
version: 1
direction: direction/reverse
top level: pSBBs3C
start: 9661
end: 10323
orientation: inline

luxA
type: SequenceAnnotation
persistent identity: annotation2180247
display id: annotation2180247
version: 1
top level: pSBBs3C
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823025/annotation2180247/range2180247/1

https://synbiohub.org/public/igem/BBa_K823025/annotation2180247/range2180247/1
type: Range
persistent identity: range2180247
display id: range2180247
version: 1
direction: direction/forward
top level: pSBBs3C
start: 73
end: 1155
orientation: inline

luxB
type: SequenceAnnotation
persistent identity: annotation2180248
display id: annotation2180248
version: 1
top level: pSBBs3C
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823025/annotation2180248/range2180248/1

https://synbiohub.org/public/igem/BBa_K823025/annotation2180248/range2180248/1
type: Range
persistent identity: range2180248
display id: range2180248
version: 1
direction: direction/forward
top level: pSBBs3C
start: 1172
end: 2155
orientation: inline

sacA
type: SequenceAnnotation
persistent identity: annotation2180252
display id: annotation2180252
version: 1
top level: pSBBs3C
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823025/annotation2180252/range2180252/1

https://synbiohub.org/public/igem/BBa_K823025/annotation2180252/range2180252/1
type: Range
persistent identity: range2180252
display id: range2180252
version: 1
direction: direction/forward
top level: pSBBs3C
start: 5676
end: 6515
orientation: inline

luxC
type: SequenceAnnotation
persistent identity: annotation2180249
display id: annotation2180249
version: 1
top level: pSBBs3C
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823025/annotation2180249/range2180249/1

https://synbiohub.org/public/igem/BBa_K823025/annotation2180249/range2180249/1
type: Range
persistent identity: range2180249
display id: range2180249
version: 1
direction: direction/forward
top level: pSBBs3C
start: 2179
end: 3621
orientation: inline

luxE
type: SequenceAnnotation
persistent identity: annotation2180251
display id: annotation2180251
version: 1
top level: pSBBs3C
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823025/annotation2180251/range2180251/1

https://synbiohub.org/public/igem/BBa_K823025/annotation2180251/range2180251/1
type: Range
persistent identity: range2180251
display id: range2180251
version: 1
direction: direction/forward
top level: pSBBs3C
start: 4583
end: 5695
orientation: inline

sacA
type: SequenceAnnotation
persistent identity: annotation2180254
display id: annotation2180254
version: 1
top level: pSBBs3C
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823025/annotation2180254/range2180254/1

https://synbiohub.org/public/igem/BBa_K823025/annotation2180254/range2180254/1
type: Range
persistent identity: range2180254
display id: range2180254
version: 1
direction: direction/forward
top level: pSBBs3C
start: 8712
end: 9503
orientation: inline

luxD
type: SequenceAnnotation
persistent identity: annotation2180250
display id: annotation2180250
version: 1
top level: pSBBs3C
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823025/annotation2180250/range2180250/1

https://synbiohub.org/public/igem/BBa_K823025/annotation2180250/range2180250/1
type: Range
persistent identity: range2180250
display id: range2180250
version: 1
direction: direction/forward
top level: pSBBs3C
start: 3635
end: 4558
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2180253
display id: annotation2180253
version: 1
top level: pSBBs3C
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823025/annotation2180253/range2180253/1

https://synbiohub.org/public/igem/BBa_K823025/annotation2180253/range2180253/1
type: Range
persistent identity: range2180253
display id: range2180253
version: 1
direction: direction/forward
top level: pSBBs3C
start: 6849
end: 7721
orientation: inline

pSBBs4S
type: ComponentDefinition
persistent identity: BBa K823022
display id: BBa_K823022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSBBs4S: Empty backbone for integration into Bacillus subtilis thrC locus
creator: Jara Radeck
created: 2012-08-15T11:00:00Z
modified: 2015-05-08T01:13:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 0
owner id: 6378
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is an empty backbone vector for the usage in Bacillus subtilis. It integrates in the thrC locus and can be selected with Spectinomycin cassette. It has a ampicillin resistance for cloning in E.coli. It originates from the Bacillus vector pDG1731.
mutable notes: This plasmid can be used as empty backbone vectors like pSB1C3 but is able to integrate in B.subtilis. The clonging site also includes a rfp for selection.
mutable provenance: B.subtilis vector pDG1731
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: ori approx.
sequence annotation: thrC'
sequence annotation: bla
sequence annotation: thrB
sequence annotation: 'thrC
sequence annotation: spc
sequence annotation: hom
sequence: https://synbiohub.org/public/igem/BBa_K823022_sequence/1

ori approx.
type: SequenceAnnotation
persistent identity: annotation2179610
display id: annotation2179610
version: 1
top level: pSBBs4S
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823022/annotation2179610/range2179610/1

https://synbiohub.org/public/igem/BBa_K823022/annotation2179610/range2179610/1
type: Range
persistent identity: range2179610
display id: range2179610
version: 1
direction: direction/forward
top level: pSBBs4S
start: 1942
end: 2556
orientation: inline

thrC'
type: SequenceAnnotation
persistent identity: annotation2179613
display id: annotation2179613
version: 1
top level: pSBBs4S
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823022/annotation2179613/range2179613/1

https://synbiohub.org/public/igem/BBa_K823022/annotation2179613/range2179613/1
type: Range
persistent identity: range2179613
display id: range2179613
version: 1
direction: direction/forward
top level: pSBBs4S
start: 4033
end: 4542
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2179611
display id: annotation2179611
version: 1
top level: pSBBs4S
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823022/annotation2179611/range2179611/1

https://synbiohub.org/public/igem/BBa_K823022/annotation2179611/range2179611/1
type: Range
persistent identity: range2179611
display id: range2179611
version: 1
direction: direction/reverse
top level: pSBBs4S
start: 2716
end: 3576
orientation: inline

thrB
type: SequenceAnnotation
persistent identity: annotation2179609
display id: annotation2179609
version: 1
top level: pSBBs4S
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823022/annotation2179609/range2179609/1

https://synbiohub.org/public/igem/BBa_K823022/annotation2179609/range2179609/1
type: Range
persistent identity: range2179609
display id: range2179609
version: 1
direction: direction/forward
top level: pSBBs4S
start: 1453
end: 1863
orientation: inline

'thrC
type: SequenceAnnotation
persistent identity: annotation2179608
display id: annotation2179608
version: 1
top level: pSBBs4S
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823022/annotation2179608/range2179608/1

https://synbiohub.org/public/igem/BBa_K823022/annotation2179608/range2179608/1
type: Range
persistent identity: range2179608
display id: range2179608
version: 1
direction: direction/forward
top level: pSBBs4S
start: 1028
end: 1456
orientation: inline

spc
type: SequenceAnnotation
persistent identity: annotation2179607
display id: annotation2179607
version: 1
top level: pSBBs4S
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823022/annotation2179607/range2179607/1

https://synbiohub.org/public/igem/BBa_K823022/annotation2179607/range2179607/1
type: Range
persistent identity: range2179607
display id: range2179607
version: 1
direction: direction/forward
top level: pSBBs4S
start: 211
end: 885
orientation: inline

hom
type: SequenceAnnotation
persistent identity: annotation2179612
display id: annotation2179612
version: 1
top level: pSBBs4S
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823022/annotation2179612/range2179612/1

https://synbiohub.org/public/igem/BBa_K823022/annotation2179612/range2179612/1
type: Range
persistent identity: range2179612
display id: range2179612
version: 1
direction: direction/forward
top level: pSBBs4S
start: 3731
end: 4033
orientation: inline

BBa_K090403
type: ComponentDefinition
persistent identity: BBa K090403
display id: BBa_K090403
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Gram-positive Shuttle Vector for Chromosomal Integration
creator: Daniel Goodman
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _188_
m user id: 0
owner id: 3501
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is pBSINT1, a BioBrick-compatible Shuttle Vector that allows for single-copy chromosomal insertion into Bacillus subtilis. It contains the replication origin and Biobrick cut site from pSB1A3 for normal replication in E. coli. It also holds the 5' and 3' flanking regions of the AmyE gene in Bacillus subtilis, and uses these regions of homology to integrate and replace the contents of that locus on the chromosome via a double crossover event. It has two selectable resistance markers, one for Chloramphenicol (5??g/ml) in Bacillus subtiis, and one for Ampicillin (100 ??g/ml) in E. coli. This vector can be used to construct large BioBrick parts using traditional gram-negative-based transformation and minipreps, and then also be used to integrate the completed part into the genome of a gram-positive chassis. As the figure explains, this vector was made via InFusion assembly from several pieces at once. For a detailed protocol of transformation in Bacillus and how to detect integration events at the AmyE locus, see the Cambridge iGEM 2008 wiki protocols page: [http://2008.igem.org/Team:Cambridge/Bacillus_subtilis_transformation]
mutable notes: We attempted to design the PCR ends to not interfere with the AmyE flanking regions, and also to have the same annealing temperatures. To see the primers we used, see our Primer page under the Bacillus subproject: [http://2008.igem.org/Team:Cambridge/Bacillus/Primers]
mutable provenance: As explained in the image, this vector was assembled at once from 4 separate pieces. The green and red regions (biobrick cut site and E. coli replication/Amp resistance) come from PBS1A3. The blue pieces are the flanking AmyE regions from ECE112, which was obtained via the Bacillus Genetic Stock Center.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence annotation: beta-lactamase (ampR) promoter
sequence annotation: pBR322 origin
sequence annotation: beta-lactamase (ampicillin resistance)
sequence annotation: BioBrick prefix
sequence annotation: amyE back; partial alpha-amylase; similar to Genbank U46196
sequence annotation: BioBrick suffix
sequence annotation: chloramphenicol acetyltransferase
sequence: https://synbiohub.org/public/igem/BBa_K090403_sequence/1

beta-lactamase (ampR) promoter
type: SequenceAnnotation
persistent identity: annotation1999624
display id: annotation1999624
version: 1
top level: BBa_K090403
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K090403/annotation1999624/range1999624/1

https://synbiohub.org/public/igem/BBa_K090403/annotation1999624/range1999624/1
type: Range
persistent identity: range1999624
display id: range1999624
version: 1
direction: direction/reverse
top level: BBa_K090403
start: 4746
end: 4774
orientation: inline

pBR322 origin
type: SequenceAnnotation
persistent identity: annotation1999622
display id: annotation1999622
version: 1
top level: BBa_K090403
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K090403/annotation1999622/range1999622/1

https://synbiohub.org/public/igem/BBa_K090403/annotation1999622/range1999622/1
type: Range
persistent identity: range1999622
display id: range1999622
version: 1
direction: direction/reverse
top level: BBa_K090403
start: 3070
end: 3689
orientation: inline

beta-lactamase (ampicillin resistance)
type: SequenceAnnotation
persistent identity: annotation1999623
display id: annotation1999623
version: 1
top level: BBa_K090403
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K090403/annotation1999623/range1999623/1

https://synbiohub.org/public/igem/BBa_K090403/annotation1999623/range1999623/1
type: Range
persistent identity: range1999623
display id: range1999623
version: 1
direction: direction/reverse
top level: BBa_K090403
start: 3844
end: 4704
orientation: inline

BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation1999619
display id: annotation1999619
version: 1
top level: BBa_K090403
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K090403/annotation1999619/range1999619/1

https://synbiohub.org/public/igem/BBa_K090403/annotation1999619/range1999619/1
type: Range
persistent identity: range1999619
display id: range1999619
version: 1
direction: direction/forward
top level: BBa_K090403
start: 1
end: 21
orientation: inline

amyE back; partial alpha-amylase; similar to Genbank U46196
type: SequenceAnnotation
persistent identity: annotation1999621
display id: annotation1999621
version: 1
top level: BBa_K090403
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K090403/annotation1999621/range1999621/1

https://synbiohub.org/public/igem/BBa_K090403/annotation1999621/range1999621/1
type: Range
persistent identity: range1999621
display id: range1999621
version: 1
direction: direction/forward
top level: BBa_K090403
start: 1449
end: 2468
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation1999625
display id: annotation1999625
version: 1
top level: BBa_K090403
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K090403/annotation1999625/range1999625/1

https://synbiohub.org/public/igem/BBa_K090403/annotation1999625/range1999625/1
type: Range
persistent identity: range1999625
display id: range1999625
version: 1
direction: direction/forward
top level: BBa_K090403
start: 5611
end: 5632
orientation: inline

chloramphenicol acetyltransferase
type: SequenceAnnotation
persistent identity: annotation1999620
display id: annotation1999620
version: 1
top level: BBa_K090403
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K090403/annotation1999620/range1999620/1

https://synbiohub.org/public/igem/BBa_K090403/annotation1999620/range1999620/1
type: Range
persistent identity: range1999620
display id: range1999620
version: 1
direction: direction/forward
top level: BBa_K090403
start: 419
end: 1069
orientation: inline

BBa_K1973006
type: ComponentDefinition
persistent identity: BBa K1973006
display id: BBa_K1973006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: miniTn7BB-Gm-nahR-Psal-lapG
creator: Gabriel Ruiz Romero
created: 2016-10-12T11:00:00Z
modified: 2016-10-13T09:24:18Z
discontinued: false
dominant: false
group u list: _2440_
m user id: 29968
owner id: 29968
owning group id: 9
status: status/Planning
bookmark: false
mutable description: nahR (Pseudomonas putida, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the Psal promoter (see BBa_J61051) in the presence of salicylate. lapG (see BBa_K1973000) is expressed from the Psal promoter in the presence of this molecule. The miniTn7 tool allows the stable integration of this module in the chromosome of a wide range of bacteria (see http://parts.igem.org/Genome_Integration).
mutable notes: Designs are described in basic parts pages
mutable provenance: Basic parts were cloned into the miniTn7BB-Gm Backbone
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K510000
component: BBa_K1973033
component: BBa_K1973000
sequence annotation: BBa_K1973033
sequence annotation: BBa_K510000
sequence annotation: BBa_K1973000
sequence: https://synbiohub.org/public/igem/BBa_K1973006_sequence/1

BBa_K510000
type: Component
persistent identity: component2498468
display id: component2498468
version: 1
top level: BBa_K1973006
access: public
definition: BBa_K510000

BBa_K1973033
type: Component
persistent identity: component2498471
display id: component2498471
version: 1
top level: BBa_K1973006
access: public
definition: https://synbiohub.org/public/igem/BBa_K1973033/1

BBa_K1973000
type: Component
persistent identity: component2498475
display id: component2498475
version: 1
top level: BBa_K1973006
access: public
definition: https://synbiohub.org/public/igem/BBa_K1973000/1

BBa_K1973033
type: SequenceAnnotation
persistent identity: annotation2498471
display id: annotation2498471
version: 1
top level: BBa_K1973006
location: https://synbiohub.org/public/igem/BBa_K1973006/annotation2498471/range2498471/1
component: BBa_K1973033

https://synbiohub.org/public/igem/BBa_K1973006/annotation2498471/range2498471/1
type: Range
persistent identity: range2498471
display id: range2498471
version: 1
direction: direction/reverse
top level: BBa_K1973006
start: 4397
end: 5435
orientation: inline

BBa_K510000
type: SequenceAnnotation
persistent identity: annotation2498468
display id: annotation2498468
version: 1
top level: BBa_K1973006
location: https://synbiohub.org/public/igem/BBa_K1973006/annotation2498468/range2498468/1
component: BBa_K510000

https://synbiohub.org/public/igem/BBa_K1973006/annotation2498468/range2498468/1
type: Range
persistent identity: range2498468
display id: range2498468
version: 1
direction: direction/reverse
top level: BBa_K1973006
start: 1
end: 4388
orientation: inline

BBa_K1973000
type: SequenceAnnotation
persistent identity: annotation2498475
display id: annotation2498475
version: 1
top level: BBa_K1973006
location: https://synbiohub.org/public/igem/BBa_K1973006/annotation2498475/range2498475/1
component: BBa_K1973000

https://synbiohub.org/public/igem/BBa_K1973006/annotation2498475/range2498475/1
type: Range
persistent identity: range2498475
display id: range2498475
version: 1
direction: direction/reverse
top level: BBa_K1973006
start: 5444
end: 6106
orientation: inline

BBa_K863201
type: ComponentDefinition
persistent identity: BBa K863201
display id: BBa_K863201
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3' UTR site of alcohol oxidase 1 gene (aox1)
creator: Julia Schirmacher
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1123_
m user id: 0
owner id: 13076
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: 3' UTR site of alcohol oxidase 1 gene (AOX1) from K. pastoris used as a recombination site.
mutable notes: primer sequences are coming soon
mutable provenance: The DNA sequence from plasmid pPIC9K of Invitrogen (http://products.invitrogen.com/ivgn/product/V17520?ICID==%3Dsearch-product) was used for primer design. But the genomic DNA of Pichia pastoris wildtype X33 was used for amplification via PCR.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K863201_sequence/1

p68
type: ComponentDefinition
persistent identity: BBa K678070
display id: BBa_K678070
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: p68
creator: DTU-Denmark-2
created: 2011-09-12T11:00:00Z
modified: 2015-05-08T01:13:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: To be announced
mutable notes: To be announced
mutable provenance: To be announced
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence annotation: TtrpC
sequence annotation: lacZ
sequence annotation: IS-down
sequence annotation: ampR
sequence annotation: USER cassette (AsiSI/Nb.BtsI)
sequence annotation: argB marker cassette
sequence annotation: IS-up
sequence: https://synbiohub.org/public/igem/BBa_K678070_sequence/1

TtrpC
type: SequenceAnnotation
persistent identity: annotation2135109
display id: annotation2135109
version: 1
top level: p68
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K678070/annotation2135109/range2135109/1

https://synbiohub.org/public/igem/BBa_K678070/annotation2135109/range2135109/1
type: Range
persistent identity: range2135109
display id: range2135109
version: 1
direction: direction/forward
top level: p68
start: 6396
end: 7157
orientation: inline

lacZ
type: SequenceAnnotation
persistent identity: annotation2135108
display id: annotation2135108
version: 1
top level: p68
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K678070/annotation2135108/range2135108/1

https://synbiohub.org/public/igem/BBa_K678070/annotation2135108/range2135108/1
type: Range
persistent identity: range2135108
display id: range2135108
version: 1
direction: direction/forward
top level: p68
start: 3319
end: 6392
orientation: inline

IS-down
type: SequenceAnnotation
persistent identity: annotation2135111
display id: annotation2135111
version: 1
top level: p68
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678070/annotation2135111/range2135111/1

https://synbiohub.org/public/igem/BBa_K678070/annotation2135111/range2135111/1
type: Range
persistent identity: range2135111
display id: range2135111
version: 1
direction: direction/forward
top level: p68
start: 9342
end: 11267
orientation: inline

ampR
type: SequenceAnnotation
persistent identity: annotation2135105
display id: annotation2135105
version: 1
top level: p68
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678070/annotation2135105/range2135105/1

https://synbiohub.org/public/igem/BBa_K678070/annotation2135105/range2135105/1
type: Range
persistent identity: range2135105
display id: range2135105
version: 1
direction: direction/forward
top level: p68
start: 1
end: 859
orientation: inline

USER cassette (AsiSI/Nb.BtsI)
type: SequenceAnnotation
persistent identity: annotation2135107
display id: annotation2135107
version: 1
top level: p68
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678070/annotation2135107/range2135107/1

https://synbiohub.org/public/igem/BBa_K678070/annotation2135107/range2135107/1
type: Range
persistent identity: range2135107
display id: range2135107
version: 1
direction: direction/forward
top level: p68
start: 3285
end: 3318
orientation: inline

argB marker cassette
type: SequenceAnnotation
persistent identity: annotation2135110
display id: annotation2135110
version: 1
top level: p68
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678070/annotation2135110/range2135110/1

https://synbiohub.org/public/igem/BBa_K678070/annotation2135110/range2135110/1
type: Range
persistent identity: range2135110
display id: range2135110
version: 1
direction: direction/forward
top level: p68
start: 7171
end: 9327
orientation: inline

IS-up
type: SequenceAnnotation
persistent identity: annotation2135106
display id: annotation2135106
version: 1
top level: p68
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678070/annotation2135106/range2135106/1

https://synbiohub.org/public/igem/BBa_K678070/annotation2135106/range2135106/1
type: Range
persistent identity: range2135106
display id: range2135106
version: 1
direction: direction/forward
top level: p68
start: 1318
end: 3266
orientation: inline

BBa_K1976001
type: ComponentDefinition
persistent identity: BBa K1976001
display id: BBa_K1976001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: λ-Integrase
creator: Thomas Dohmen, Milana Kremenovic, Yannick Kristiansen, Tim Maier
created: 2016-10-13T11:00:00Z
modified: 2016-10-19T05:26:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2443_
m user id: 30857
owner id: 26981
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Helping plasmid for genomic integration
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: J23101
sequence annotation: B0034
sequence annotation: lambda-INT
sequence: https://synbiohub.org/public/igem/BBa_K1976001_sequence/1

J23101
type: SequenceAnnotation
persistent identity: annotation2517065
display id: annotation2517065
version: 1
top level: BBa_K1976001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1976001/annotation2517065/range2517065/1

https://synbiohub.org/public/igem/BBa_K1976001/annotation2517065/range2517065/1
type: Range
persistent identity: range2517065
display id: range2517065
version: 1
direction: direction/forward
top level: BBa_K1976001
start: 1
end: 35
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2517066
display id: annotation2517066
version: 1
top level: BBa_K1976001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1976001/annotation2517066/range2517066/1

https://synbiohub.org/public/igem/BBa_K1976001/annotation2517066/range2517066/1
type: Range
persistent identity: range2517066
display id: range2517066
version: 1
direction: direction/forward
top level: BBa_K1976001
start: 42
end: 53
orientation: inline

lambda-INT
type: SequenceAnnotation
persistent identity: annotation2517067
display id: annotation2517067
version: 1
top level: BBa_K1976001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1976001/annotation2517067/range2517067/1

https://synbiohub.org/public/igem/BBa_K1976001/annotation2517067/range2517067/1
type: Range
persistent identity: range2517067
display id: range2517067
version: 1
direction: direction/forward
top level: BBa_K1976001
start: 54
end: 1124
orientation: inline