##gff-version 3
##sequence-region pSB4K5 1 3419
pSB4K5	.	terminator	3203	3274	.	+	0	ID=BBa_B0053;Name=BBa_B0053
pSB4K5	.	stem_loop	3211	3245	.	+	0	ID=annotation318602;Name=stem_loop;Parent=BBa_B0053
pSB4K5	.	sequence_feature	1	21	.	+	0	ID=BBa_G00001;Name=BBa_G00001
pSB4K5	.	sequence_feature	2	7	.	+	0	ID=annotation1934226;Name=SpeI;Parent=BBa_G00001
pSB4K5	.	engineered_region	1	21	.	+	0	ID=annotation1821544;Name=Suffix;Parent=BBa_G00001
pSB4K5	.	sequence_feature	16	21	.	+	0	ID=annotation1934227;Name=PstI;Parent=BBa_G00001
pSB4K5	.	sequence_feature	9	16	.	+	0	ID=annotation1934228;Name=NotI;Parent=BBa_G00001
pSB4K5	.	sequence_feature	35	46	.	+	0	ID=BBa_B0042;Name=BBa_B0042
pSB4K5	.	stop_codon	35	37	.	+	0	ID=annotation1797084;Name=stop codon (-1 frame);Parent=BBa_B0042
pSB4K5	.	stop_codon	36	38	.	+	0	ID=annotation1797083;Name=stop codon (+2 frame);Parent=BBa_B0042
pSB4K5	.	stop_codon	40	42	.	+	0	ID=annotation1797085;Name=stop codon (+3 frame);Parent=BBa_B0042
pSB4K5	.	stop_codon	44	46	.	+	0	ID=annotation1797086;Name=stop codon (+1 frame);Parent=BBa_B0042
pSB4K5	.	stop_codon	39	41	.	+	0	ID=annotation1797088;Name=stop codon (-3 frame);Parent=BBa_B0042
pSB4K5	.	stop_codon	43	45	.	+	0	ID=annotation1797087;Name=stop codon (-2 frame);Parent=BBa_B0042
pSB4K5	.	sequence_feature	22	34	.	+	0	ID=BBa_B0044;Name=BBa_B0044
pSB4K5	.	sequence_feature	22	26	.	+	0	ID=annotation1797126;Name=Nt.BstNB I recognition site;Parent=BBa_B0044
pSB4K5	.	sequence_feature	30	34	.	+	0	ID=annotation1797128;Name=Topoisomerase I recognition site;Parent=BBa_B0044
pSB4K5	.	sequence_feature	27	29	.	+	0	ID=annotation1797127;Name=Arbitrary sequence (could be any bases);Parent=BBa_B0044
pSB4K5	.	sequence_feature	30	30	.	+	0	ID=annotation1797129;Name=Generated T overhang;Parent=BBa_B0044
pSB4K5	.	sequence_feature	177	183	.	+	0	ID=BBa_G0005;Name=BBa_G0005
pSB4K5	.	sequence_feature	3373	3384	.	+	0	ID=BBa_B0042;Name=BBa_B0042
pSB4K5	.	stop_codon	3373	3375	.	+	0	ID=annotation1797084;Name=stop codon (-1 frame);Parent=BBa_B0042
pSB4K5	.	stop_codon	3374	3376	.	+	0	ID=annotation1797083;Name=stop codon (+2 frame);Parent=BBa_B0042
pSB4K5	.	stop_codon	3378	3380	.	+	0	ID=annotation1797085;Name=stop codon (+3 frame);Parent=BBa_B0042
pSB4K5	.	stop_codon	3382	3384	.	+	0	ID=annotation1797086;Name=stop codon (+1 frame);Parent=BBa_B0042
pSB4K5	.	stop_codon	3377	3379	.	+	0	ID=annotation1797088;Name=stop codon (-3 frame);Parent=BBa_B0042
pSB4K5	.	stop_codon	3381	3383	.	+	0	ID=annotation1797087;Name=stop codon (-2 frame);Parent=BBa_B0042
pSB4K5	.	engineered_region	184	1150	.	+	0	ID=BBa_P1007;Name=BBa_P1007
pSB4K5	.	point_mutation	970	970	.	+	0	ID=annotation1919400;Name=G to C mutation to eliminate XhoI site;Parent=BBa_P1007
pSB4K5	.	sequence_feature	1107	1150	.	+	0	ID=annotation1919401;Name=repeat region;Parent=BBa_P1007
pSB4K5	.	CDS	184	1002	.	+	0	ID=annotation1919399;Name=kanamycin resistance;Parent=BBa_P1007
pSB4K5	.	sequence_feature	3385	3397	.	+	0	ID=BBa_B0043;Name=BBa_B0043
pSB4K5	.	sequence_feature	3389	3389	.	+	0	ID=annotation1797104;Name=Generated T overhang;Parent=BBa_B0043
pSB4K5	.	sequence_feature	3393	3397	.	+	0	ID=annotation1797102;Name=Nt.BstNB I recognition site;Parent=BBa_B0043
pSB4K5	.	sequence_feature	3390	3392	.	+	0	ID=annotation1797103;Name=Arbitrary sequence (could be any bases);Parent=BBa_B0043
pSB4K5	.	sequence_feature	3385	3389	.	+	0	ID=annotation1797101;Name=Topoisomerase I recognition site;Parent=BBa_B0043
pSB4K5	.	terminator	3295	3372	.	+	0	ID=BBa_B0055;Name=BBa_B0055
pSB4K5	.	sequence_feature	3322	3360	.	+	0	ID=annotation1783311;Name=identical to bacteriophage T3;Parent=BBa_B0055
pSB4K5	.	stem_loop	3330	3351	.	+	0	ID=annotation1783301;Name=stem loop from bacteriophage T3;Parent=BBa_B0055
pSB4K5	.	terminator	47	115	.	+	0	ID=BBa_B0054;Name=BBa_B0054
pSB4K5	.	stem_loop	57	88	.	+	0	ID=annotation1747359;Name=stem loop;Parent=BBa_B0054
pSB4K5	.	sequence_feature	50	89	.	+	0	ID=annotation1783342;Name=identical to E. coli MG1655 between tonB and yciA;Parent=BBa_B0054
pSB4K5	.	sequence_feature	1159	3195	.	+	0	ID=BBa_I50041;Name=BBa_I50041
pSB4K5	.	sequence_alteration	2377	2382	.	+	0	ID=annotation1932224;Name=Mutated SpeI site;Parent=BBa_I50041
pSB4K5	.	sequence_feature	3015	3031	.	+	0	ID=annotation1932232;Name=inverted repeat;Parent=BBa_I50041
pSB4K5	.	ribosome_entry_site	2312	2317	.	+	0	ID=annotation1932218;Name=RBS;Parent=BBa_I50041
pSB4K5	.	polyA_site	1234	1249	.	+	0	ID=annotation1932215;Name=putative polyA;Parent=BBa_I50041
pSB4K5	.	sequence_feature	2958	2975	.	+	0	ID=annotation1932230;Name=inverted repeat;Parent=BBa_I50041
pSB4K5	.	sequence_feature	2440	2459	.	+	0	ID=annotation1932226;Name=direct repeat;Parent=BBa_I50041
pSB4K5	.	sequence_feature	3034	3038	.	+	0	ID=annotation1932233;Name=Tn3 integration site;Parent=BBa_I50041
pSB4K5	.	sequence_feature	2479	2561	.	+	0	ID=annotation1932228;Name=AT rich region;Parent=BBa_I50041
pSB4K5	.	ribosome_entry_site	2314	2326	.	+	0	ID=annotation1932219;Name=RBS;Parent=BBa_I50041
pSB4K5	.	promoter	2331	2350	.	+	0	ID=annotation1932220;Name=promoter region;Parent=BBa_I50041
pSB4K5	.	sequence_feature	2461	2480	.	+	0	ID=annotation1932227;Name=direct repeat;Parent=BBa_I50041
pSB4K5	.	sequence_feature	2408	2427	.	+	0	ID=annotation1932225;Name=direct repeat;Parent=BBa_I50041
pSB4K5	.	promoter	2341	2346	.	+	0	ID=annotation1932222;Name=rep promoter -10;Parent=BBa_I50041
pSB4K5	.	stem_loop	3121	3150	.	+	0	ID=annotation1932235;Name=terminator;Parent=BBa_I50041
pSB4K5	.	sequence_feature	2692	3059	.	+	0	ID=annotation1932229;Name=par region;Parent=BBa_I50041
pSB4K5	.	sequence_alteration	1194	1194	.	+	0	ID=annotation2080856;Name=difference from canonical pSC101 sequence in Genbank;Parent=BBa_I50041
pSB4K5	.	sequence_feature	2337	2358	.	+	0	ID=annotation1932221;Name=inverted repeat;Parent=BBa_I50041
pSB4K5	.	sequence_alteration	1541	1541	.	+	0	ID=annotation2080859;Name=difference from canonical pSC101 sequence in Genbank;Parent=BBa_I50041
pSB4K5	.	sequence_feature	2312	2334	.	+	0	ID=annotation1932217;Name=inverted repeat;Parent=BBa_I50041
pSB4K5	.	CDS	1360	2310	.	+	0	ID=annotation1932216;Name=repA;Parent=BBa_I50041
pSB4K5	.	sequence_feature	3044	3057	.	+	0	ID=annotation1932234;Name=inverted repeat;Parent=BBa_I50041
pSB4K5	.	promoter	2363	2368	.	+	0	ID=annotation1932223;Name=rep promoter -35;Parent=BBa_I50041
pSB4K5	.	sequence_feature	2981	3004	.	+	0	ID=annotation1932231;Name=inverted repeat;Parent=BBa_I50041
pSB4K5	.	sequence_alteration	1284	1284	.	+	0	ID=annotation2080857;Name=difference from canonical pSC101 sequence in Genbank;Parent=BBa_I50041
pSB4K5	.	sequence_alteration	1446	1446	.	+	0	ID=annotation2080858;Name=difference from canonical pSC101 sequence in Genbank;Parent=BBa_I50041
pSB4K5	.	terminator	136	176	.	+	0	ID=BBa_B0062;Name=BBa_B0062
pSB4K5	.	stem_loop	149	168	.	+	0	ID=annotation318637;Name=stem_loop;Parent=BBa_B0062
pSB4K5	.	sequence_feature	1151	1158	.	+	0	ID=BBa_G0001;Name=BBa_G0001
pSB4K5	.	sequence_feature	116	135	.	+	0	ID=BBa_G00102;Name=BBa_G00102
pSB4K5	.	primer_binding_site	116	135	.	+	0	ID=annotation1961229;Name=VR;Parent=BBa_G00102
pSB4K5	.	sequence_feature	3398	3419	.	+	0	ID=BBa_G00000;Name=BBa_G00000
pSB4K5	.	sequence_feature	3404	3411	.	+	0	ID=annotation1961228;Name=NotI site;Parent=BBa_G00000
pSB4K5	.	sequence_feature	3398	3403	.	+	0	ID=annotation1934223;Name=EcoRI site;Parent=BBa_G00000
pSB4K5	.	sequence_feature	3413	3418	.	+	0	ID=annotation1934224;Name=XbaI site;Parent=BBa_G00000
pSB4K5	.	engineered_region	3398	3419	.	+	0	ID=annotation1934225;Name=Prefix;Parent=BBa_G00000
pSB4K5	.	primer	3275	3294	.	+	0	ID=BBa_G00100;Name=BBa_G00100
pSB4K5	.	primer_binding_site	3275	3294	.	+	0	ID=annotation1934505;Name=VF2;Parent=BBa_G00100
pSB4K5	.	sequence_feature	3196	3202	.	+	0	ID=BBa_G0006;Name=BBa_G0006
>pSB4K5
tactagtagcggccgctgcaggagtcactaagggttagttagttagattagcagaaagtcaaaagcctccgaccggagg
cttttgactaaaacttcccttggggttatcattggggctcactcaaaggcggtaatcagataaaaaaaatccttagctt
tcgctaaggatgatttctgctagtattattagaaaaactcatcgagcatcaaatgaaactgcaatttattcatatcagg
attatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaaactcaccgaggcagttccaaagaatggca
aggtcctggtaacggtctgcgattccgacccgtccaacatcaatacaacctattaatttcccctcgtcaaaaataaggt
tatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaagagcttgtgcatttctttccagacttg
ttcaacaggccagccattacgctcgtcatcaaaatcactcgcatcaaccaaaccgttattcatgcgtgattgcgcctga
gcaagacgaaatacacgatcgctgttaaaaggacaattacaaacaggaatcgaatgtaaccggcgcaggaacacggcca
gcgcatcaacaatattttcacctgaatcaggatattcttctaatacctggaaggctgttttcccaggaatcgcggtggt
gagtaaccacgcatcatcaggagtacggataaaatgcttgatggtcgggagaggcataaactccgtcagccagttgaga
cggaccatctcatctgtaacatcattggcaacgctacctttgccatgtttcagaaacaactctggcgcatcgggcttcc
catacaagcgatagattgtcgcacctgattgcccgacattatcgcgagcccatttatacccatataaatcagcgtccat
gttggagtttaagcgcggacgggagcaagacgtttcccgttgaatatggctcataacaccccttgtattactgtttatg
taagcagacagttttattgttcatgatgatatatttttatcttgtgcaatgtaacatcagagattttgagacacaacgt
ggctttgttgaataaatcgaacttttgctgagttgaaggatcagctctagtagttacattgtcgatctgttcatggtga
acagctttgaatgcaccaaaaactcgtaaaagctctgatgtatctatcttttttacaccgttttcatctgtgcatatgg
acagttttccctttgatatgtaacggtgaacagttgttctacttttgtttgttagtcttgatgcttcactgatagatac
aagagccataagaacctcagatccttccgtatttagccagtatgttctctagtgtggttcgttgtttttgcgtgagcca
tgagaacgaaccattgagatcatacttactttgcatgtcactcaaaaattttgcctcaaaactggtgagctgaattttt
gcagttaaagcatcgtgtagtgtttttcttagtccgttatgtaggtaggaatctgatgtaatggttgttggtattttgt
caccattcatttttatctggttgttctcaagttcggttacgagatccatttgtctatctagttcaacttggaaaatcaa
cgtatcagtcgggcggcctcgcttatcaaccaccaatttcatattgctgtaagtgtttaaatctttacttattggtttc
aaaacccattggttaagccttttaaactcatggtagttattttcaagcattaacatgaacttaaattcatcaaggctaa
tctctatatttgccttgtgagttttcttttgtgttagttcttttaataaccactcataaatcctcatagagtatttgtt
ttcaaaagacttaacatgttccagattatattttatgaatttttttaactggaaaagataaggcaatatctcttcacta
aaaactaattctaatttttcgcttgagaacttggcatagtttgtccactggaaaatctcaaagcctttaaccaaaggat
tcctgatttccacagttctcgtcatcagctctctggttgctttagctaatacaccataagcattttccctactgatgtt
catcatctgagcgtattggttataagtgaacgataccgtccgttctttccttgtagggttttcaatcgtggggttgagt
agtgccacacagcataaaattagcttggtttcatgctccgttaagtcatagcgactaatcgctagttcatttgctttga
aaacaactaattcagacatacatctcaattggtctaggtgattttaatcactataccaattgagatgggctagtcaatg
ataattacatgtccttttcctttgagttgtgggtatctgtaaattctgctagacctttgctggaaaacttgtaaattct
gctagaccctctgtaaattccgctagacctttgtgtgttttttttgtttatattcaagtggttataatttatagaataa
agaaagaataaaaaaagataaaaagaatagatcccagccctgtgtataactcactactttagtcagttccgcagtatta
caaaaggatgtcgcaaacgctgtttgctcctctacaaaacagaccttaaaaccctaaaggcttaagtagcaccctcgca
agctcgggcaaatcgctgaatattccttttgtctccgaccatcaggcacctgagtcgctgtctttttcgtgacattcag
ttcgctgcgctcacggctctggcagtgaatgggggtaaatggcactacaggcgccttttatggattcatgcaaggaaac
tacccataatacaagaaaagcccgtcacgggcttctcagggcgttttatggcgggtctgctatgtggtgctatctgact
ttttgctgttcagcagttcctgccctctgattttccagtctgaccacttcggattatcccgtgacaggtcattcagact
ggctaatgcacccagtaaggcagcggtatcatcaacaggcttacccgtcttactgtccctagtgcttggattctcacca
ataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcattactggatctatcaaca
ggagtccaagcgagctcgtaaacttggtctgacagctctagctccggcaaaaaaacgggcaaggtgtcaccaccctgcc
ctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccg
tgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactc
gaattcgcggccgcttctagag