##gff-version 3
##sequence-region pSB4AC5 1 5827
pSB4AC5	.	sequence_feature	148	167	.	+	0	ID=BBa_G00102;Name=BBa_G00102
pSB4AC5	.	primer_binding_site	148	167	.	+	0	ID=annotation1961229;Name=VR;Parent=BBa_G00102
pSB4AC5	.	primer	4323	4342	.	+	0	ID=BBa_G00100;Name=BBa_G00100
pSB4AC5	.	primer_binding_site	4323	4342	.	+	0	ID=annotation1934505;Name=VF2;Parent=BBa_G00100
pSB4AC5	.	sequence_feature	51	62	.	+	0	ID=BBa_B0042;Name=BBa_B0042
pSB4AC5	.	stop_codon	60	62	.	+	0	ID=annotation1797086;Name=stop codon (+1 frame);Parent=BBa_B0042
pSB4AC5	.	stop_codon	55	57	.	+	0	ID=annotation1797088;Name=stop codon (-3 frame);Parent=BBa_B0042
pSB4AC5	.	stop_codon	59	61	.	+	0	ID=annotation1797087;Name=stop codon (-2 frame);Parent=BBa_B0042
pSB4AC5	.	stop_codon	56	58	.	+	0	ID=annotation1797085;Name=stop codon (+3 frame);Parent=BBa_B0042
pSB4AC5	.	stop_codon	51	53	.	+	0	ID=annotation1797084;Name=stop codon (-1 frame);Parent=BBa_B0042
pSB4AC5	.	stop_codon	52	54	.	+	0	ID=annotation1797083;Name=stop codon (+2 frame);Parent=BBa_B0042
pSB4AC5	.	engineered_region	1071	2011	.	+	0	ID=BBa_P1004;Name=BBa_P1004
pSB4AC5	.	CDS	1177	1839	.	+	0	ID=annotation1897114;Name=cmR;Parent=BBa_P1004
pSB4AC5	.	engineered_region	225	1013	.	+	0	ID=BBa_P1009;Name=BBa_P1009
pSB4AC5	.	CDS	248	907	.	+	0	ID=annotation1877871;Name=CmR;Parent=BBa_P1009
pSB4AC5	.	sequence_feature	1	21	.	+	0	ID=BBa_G00001;Name=BBa_G00001
pSB4AC5	.	engineered_region	1	21	.	+	0	ID=annotation1821544;Name=Suffix;Parent=BBa_G00001
pSB4AC5	.	sequence_feature	9	16	.	+	0	ID=annotation1934228;Name=NotI;Parent=BBa_G00001
pSB4AC5	.	sequence_feature	2	7	.	+	0	ID=annotation1934226;Name=SpeI;Parent=BBa_G00001
pSB4AC5	.	sequence_feature	16	21	.	+	0	ID=annotation1934227;Name=PstI;Parent=BBa_G00001
pSB4AC5	.	terminator	71	139	.	+	0	ID=BBa_B0054;Name=BBa_B0054
pSB4AC5	.	stem_loop	81	112	.	+	0	ID=annotation1747359;Name=stem loop;Parent=BBa_B0054
pSB4AC5	.	sequence_feature	74	113	.	+	0	ID=annotation1783342;Name=identical to E. coli MG1655 between tonB and yciA;Parent=BBa_B0054
pSB4AC5	.	terminator	4243	4314	.	+	0	ID=BBa_B0053;Name=BBa_B0053
pSB4AC5	.	stem_loop	4251	4285	.	+	0	ID=annotation318602;Name=stem_loop;Parent=BBa_B0053
pSB4AC5	.	plasmid	4970	5827	.	+	0	ID=BBa_I50020;Name=BBa_I50020
pSB4AC5	.	promoter	5151	5156	.	+	0	ID=annotation1793683;Name=RNAI transcript promoter -10;Parent=BBa_I50020
pSB4AC5	.	sequence_feature	5032	5584	.	+	0	ID=annotation1793681;Name=RNAII transcript;Parent=BBa_I50020
pSB4AC5	.	sequence_alteration	5272	5272	.	+	0	ID=annotation1910690;Name=C to T, nonfunctional mutation;Parent=BBa_I50020
pSB4AC5	.	promoter	4996	5001	.	+	0	ID=annotation1793686;Name=RNAII transcript promoter -35 sequence;Parent=BBa_I50020
pSB4AC5	.	sequence_feature	4985	5599	.	+	0	ID=annotation1782180;Name=rep (pMB1);Parent=BBa_I50020
pSB4AC5	.	sequence_feature	5035	5142	.	+	0	ID=annotation1793684;Name=RNAI transcript;Parent=BBa_I50020
pSB4AC5	.	sequence_alteration	5243	5243	.	+	0	ID=annotation1910689;Name=G to A, nonfunctional mutation;Parent=BBa_I50020
pSB4AC5	.	sequence_alteration	5234	5234	.	+	0	ID=annotation1910688;Name=G to A, nonfunctional mutation;Parent=BBa_I50020
pSB4AC5	.	promoter	5017	5022	.	+	0	ID=annotation1793685;Name=RNAII transcript promoter -10 sequence;Parent=BBa_I50020
pSB4AC5	.	sequence_feature	5584	5584	.	+	0	ID=annotation1782181;Name=ORI;Parent=BBa_I50020
pSB4AC5	.	promoter	5173	5178	.	+	0	ID=annotation1793682;Name=RNAI transcript promoter -35;Parent=BBa_I50020
pSB4AC5	.	sequence_alteration	5230	5230	.	+	0	ID=annotation1910687;Name=G to A, nonfunctional mutation;Parent=BBa_I50020
pSB4AC5	.	sequence_feature	4996	5584	.	+	0	ID=annotation1793680;Name=origin of replication;Parent=BBa_I50020
pSB4AC5	.	sequence_alteration	5436	5436	.	+	0	ID=annotation1910691;Name=C to T, nonfunctional mutation;Parent=BBa_I50020
pSB4AC5	.	terminator	4351	4428	.	+	0	ID=BBa_B0055;Name=BBa_B0055
pSB4AC5	.	sequence_feature	4378	4416	.	+	0	ID=annotation1783311;Name=identical to bacteriophage T3;Parent=BBa_B0055
pSB4AC5	.	stem_loop	4386	4407	.	+	0	ID=annotation1783301;Name=stem loop from bacteriophage T3;Parent=BBa_B0055
pSB4AC5	.	terminator	176	216	.	+	0	ID=BBa_B0062;Name=BBa_B0062
pSB4AC5	.	stem_loop	189	208	.	+	0	ID=annotation318637;Name=stem_loop;Parent=BBa_B0062
pSB4AC5	.	engineered_region	4508	4961	.	+	0	ID=BBa_P1011;Name=BBa_P1011
pSB4AC5	.	promoter	4548	4553	.	+	0	ID=annotation1822230;Name=-35;Parent=BBa_P1011
pSB4AC5	.	promoter	4571	4576	.	+	0	ID=annotation1822231;Name=-10;Parent=BBa_P1011
pSB4AC5	.	CDS	4615	4923	.	+	0	ID=annotation1822232;Name=ccdB;Parent=BBa_P1011
pSB4AC5	.	sequence_feature	4478	4499	.	+	0	ID=BBa_G00000;Name=BBa_G00000
pSB4AC5	.	sequence_feature	4484	4491	.	+	0	ID=annotation1961228;Name=NotI site;Parent=BBa_G00000
pSB4AC5	.	sequence_feature	4478	4483	.	+	0	ID=annotation1934223;Name=EcoRI site;Parent=BBa_G00000
pSB4AC5	.	engineered_region	4478	4499	.	+	0	ID=annotation1934225;Name=Prefix;Parent=BBa_G00000
pSB4AC5	.	sequence_feature	4493	4498	.	+	0	ID=annotation1934224;Name=XbaI site;Parent=BBa_G00000
pSB4AC5	.	terminator	1022	1062	.	+	0	ID=BBa_B0062;Name=BBa_B0062
pSB4AC5	.	stem_loop	1035	1054	.	+	0	ID=annotation318637;Name=stem_loop;Parent=BBa_B0062
pSB4AC5	.	sequence_feature	4457	4469	.	+	0	ID=BBa_B0043;Name=BBa_B0043
pSB4AC5	.	sequence_feature	4457	4461	.	+	0	ID=annotation1797101;Name=Topoisomerase I recognition site;Parent=BBa_B0043
pSB4AC5	.	sequence_feature	4462	4464	.	+	0	ID=annotation1797103;Name=Arbitrary sequence (could be any bases);Parent=BBa_B0043
pSB4AC5	.	sequence_feature	4465	4469	.	+	0	ID=annotation1797102;Name=Nt.BstNB I recognition site;Parent=BBa_B0043
pSB4AC5	.	sequence_feature	4461	4461	.	+	0	ID=annotation1797104;Name=Generated T overhang;Parent=BBa_B0043
pSB4AC5	.	sequence_feature	2020	4234	.	+	0	ID=BBa_I50041;Name=BBa_I50041
pSB4AC5	.	ribosome_entry_site	3175	3187	.	+	0	ID=annotation1932219;Name=RBS;Parent=BBa_I50041
pSB4AC5	.	sequence_alteration	2055	2055	.	+	0	ID=annotation2080856;Name=difference from canonical pSC101 sequence in Genbank;Parent=BBa_I50041
pSB4AC5	.	stem_loop	3982	4011	.	+	0	ID=annotation1932235;Name=terminator;Parent=BBa_I50041
pSB4AC5	.	ribosome_entry_site	3173	3178	.	+	0	ID=annotation1932218;Name=RBS;Parent=BBa_I50041
pSB4AC5	.	polyA_site	2095	2110	.	+	0	ID=annotation1932215;Name=putative polyA;Parent=BBa_I50041
pSB4AC5	.	sequence_feature	3842	3865	.	+	0	ID=annotation1932231;Name=inverted repeat;Parent=BBa_I50041
pSB4AC5	.	sequence_feature	3301	3320	.	+	0	ID=annotation1932226;Name=direct repeat;Parent=BBa_I50041
pSB4AC5	.	sequence_alteration	3238	3243	.	+	0	ID=annotation1932224;Name=Mutated SpeI site;Parent=BBa_I50041
pSB4AC5	.	sequence_feature	3173	3195	.	+	0	ID=annotation1932217;Name=inverted repeat;Parent=BBa_I50041
pSB4AC5	.	CDS	2221	3171	.	+	0	ID=annotation1932216;Name=repA;Parent=BBa_I50041
pSB4AC5	.	sequence_feature	3322	3341	.	+	0	ID=annotation1932227;Name=direct repeat;Parent=BBa_I50041
pSB4AC5	.	sequence_feature	3895	3899	.	+	0	ID=annotation1932233;Name=Tn3 integration site;Parent=BBa_I50041
pSB4AC5	.	promoter	3202	3207	.	+	0	ID=annotation1932222;Name=rep promoter -10;Parent=BBa_I50041
pSB4AC5	.	sequence_feature	3269	3288	.	+	0	ID=annotation1932225;Name=direct repeat;Parent=BBa_I50041
pSB4AC5	.	sequence_feature	3198	3219	.	+	0	ID=annotation1932221;Name=inverted repeat;Parent=BBa_I50041
pSB4AC5	.	sequence_feature	3553	3920	.	+	0	ID=annotation1932229;Name=par region;Parent=BBa_I50041
pSB4AC5	.	sequence_feature	3905	3918	.	+	0	ID=annotation1932234;Name=inverted repeat;Parent=BBa_I50041
pSB4AC5	.	sequence_alteration	2145	2145	.	+	0	ID=annotation2080857;Name=difference from canonical pSC101 sequence in Genbank;Parent=BBa_I50041
pSB4AC5	.	sequence_feature	3340	3422	.	+	0	ID=annotation1932228;Name=AT rich region;Parent=BBa_I50041
pSB4AC5	.	promoter	3192	3211	.	+	0	ID=annotation1932220;Name=promoter region;Parent=BBa_I50041
pSB4AC5	.	sequence_feature	3876	3892	.	+	0	ID=annotation1932232;Name=inverted repeat;Parent=BBa_I50041
pSB4AC5	.	sequence_feature	3819	3836	.	+	0	ID=annotation1932230;Name=inverted repeat;Parent=BBa_I50041
pSB4AC5	.	sequence_alteration	2402	2402	.	+	0	ID=annotation2080859;Name=difference from canonical pSC101 sequence in Genbank;Parent=BBa_I50041
pSB4AC5	.	sequence_alteration	2307	2307	.	+	0	ID=annotation2080858;Name=difference from canonical pSC101 sequence in Genbank;Parent=BBa_I50041
pSB4AC5	.	promoter	3224	3229	.	+	0	ID=annotation1932223;Name=rep promoter -35;Parent=BBa_I50041
pSB4AC5	.	sequence_feature	30	42	.	+	0	ID=BBa_B0044;Name=BBa_B0044
pSB4AC5	.	sequence_feature	35	37	.	+	0	ID=annotation1797127;Name=Arbitrary sequence (could be any bases);Parent=BBa_B0044
pSB4AC5	.	sequence_feature	38	42	.	+	0	ID=annotation1797128;Name=Topoisomerase I recognition site;Parent=BBa_B0044
pSB4AC5	.	sequence_feature	30	34	.	+	0	ID=annotation1797126;Name=Nt.BstNB I recognition site;Parent=BBa_B0044
pSB4AC5	.	sequence_feature	38	38	.	+	0	ID=annotation1797129;Name=Generated T overhang;Parent=BBa_B0044
pSB4AC5	.	sequence_feature	4437	4448	.	+	0	ID=BBa_B0042;Name=BBa_B0042
pSB4AC5	.	stop_codon	4446	4448	.	+	0	ID=annotation1797086;Name=stop codon (+1 frame);Parent=BBa_B0042
pSB4AC5	.	stop_codon	4441	4443	.	+	0	ID=annotation1797088;Name=stop codon (-3 frame);Parent=BBa_B0042
pSB4AC5	.	stop_codon	4445	4447	.	+	0	ID=annotation1797087;Name=stop codon (-2 frame);Parent=BBa_B0042
pSB4AC5	.	stop_codon	4442	4444	.	+	0	ID=annotation1797085;Name=stop codon (+3 frame);Parent=BBa_B0042
pSB4AC5	.	stop_codon	4437	4439	.	+	0	ID=annotation1797084;Name=stop codon (-1 frame);Parent=BBa_B0042
pSB4AC5	.	stop_codon	4438	4440	.	+	0	ID=annotation1797083;Name=stop codon (+2 frame);Parent=BBa_B0042
>pSB4AC5
tactagtagcggccgctgcagtactagaggagtcactaagggtactagagttagttagttagtactagagattagcaga
aagtcaaaagcctccgaccggaggcttttgactaaaacttcccttggggttatcattgggtactagaggctcactcaaa
ggcggtaattactagagcagataaaaaaaatccttagctttcgctaaggatgatttcttactagaggtccaagcgagct
cgatatcaaattacgccccgccctgccactcatcgcagtactgttgtaattcattaagcattctgccgacatggaagcc
atcacaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatggtg
aaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgaga
cgaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatat
gtgtagaaactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgtttcagtttgctcatggaaaacggtg
taacaagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacgaaattccggatgagcattcatca
ggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatc
cagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggat
atatcaacggtggtatatccagtgatttttttctccattttagcttccttagctcctgaaaatctcgataactcaaaaa
atacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgcccgatcaactactagagcagata
aaaaaaatccttagctttcgctaaggatgatttcttactagaggttaccaatgcttaatcagtgaggcacctatctcag
cgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatc
tggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccgga
agggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaa
gtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtat
ggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctcc
ttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctc
ttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcg
gcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcatt
ggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcac
ccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaa
gggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttat
tgtctcatgagcggatacatatttgaatgtatttagtactagaggtacgggttttgctgcccgcaaacgggctgttctg
gtgttgctagtttgttatcagaatcgcagatccggcttcaggtttgccggctgaaagcgctatttcttccagaattgcc
atgattttttccccacgggaggcgtcactggctcccgtgttgtcggcagctttgattcgataagcagcatcgcctgttt
caggctgtctatgtgtgactgttgagctgtaacaagttgtctcaggtgttcaatttcatgttctagttgctttgtttta
ctggtttcacctgttctattaggtgttacatgctgttcatctgttacattgtcgatctgttcatggtgaacagctttaa
atgcaccaaaaactcgtaaaagctctgatgtatctatcttttttacaccgttttcatctgtgcatatggacagttttcc
ctttgatatctaacggtgaacagttgttctacttttgtttgttagtcttgatgcttcactgatagatacaagagccata
agaacctcagatccttccgtatttagccagtatgttctctagtgtggttcgttgtttttgcgtgagccatgagaacgaa
ccattgagatcatgcttactttgcatgtcactcaaaaattttgcctcaaaactggtgagctgaatttttgcagttaaag
catcgtgtagtgtttttcttagtccgttacgtaggtaggaatctgatgtaatggttgttggtattttgtcaccattcat
ttttatctggttgttctcaagttcggttacgagatccatttgtctatctagttcaacttggaaaatcaacgtatcagtc
gggcggcctcgcttatcaaccaccaatttcatattgctgtaagtgtttaaatctttacttattggtttcaaaacccatt
ggttaagccttttaaactcatggtagttattttcaagcattaacatgaacttaaattcatcaaggctaatctctatatt
tgccttgtgagttttcttttgtgttagttcttttaataaccactcataaatcctcatagagtatttgttttcaaaagac
ttaacatgttccagattatattttatgaatttttttaactggaaaagataaggcaatatctcttcactaaaaactaatt
ctaatttttcgcttgagaacttggcatagtttgtccactggaaaatctcaaagcctttaaccaaaggattcctgatttc
cacagttctcgtcatcagctctctggttgctttagctaatacaccataagcattttccctactgatgttcatcatctga
gcgtattggttataagtgaacgataccgtccgttctttccttgtagggttttcaatcgtggggttgagtagtgccacac
agcataaaattagcttggtttcatgctccgttaagtcatagcgactaatcgctagttcatttgctttgaaaacaactaa
ttcagacatacatctcaattggtctaggtgattttaatcactataccaattgagatgggctagtcaatgataattacat
gtccttttcctttgagttgtgggtatctgtaaattctgctagacctttgctggaaaacttgtaaattctgctagaccct
ctgtaaattccgctagacctttgtgtgttttttttgtttatattcaagtggttataatttatagaataaagaaagaata
aaaaaagataaaaagaatagatcccagccctgtgtataactcactactttagtcagttccgcagtattacaaaaggatg
tcgcaaacgctgtttgctcctctacaaaacagaccttaaaaccctaaaggcttaagtagcaccctcgcaagctcgggca
aatcgctgaatattccttttgtctccgaccatcaggcacctgagtcgctgtctttttcgtgacattcagttcgctgcgc
tcacggctctggcagtgaatgggggtaaatggcactacaggcgccttttatggattcatgcaaggaaactacccataat
acaagaaaagcccgtcacgggcttctcagggcgttttatggcgggtctgctatgtggtgctatctgactttttgctgtt
cagcagttcctgccctctgattttccagtctgaccacttcggattatcccgtgacaggtcattcagactggctaatgca
cccagtaaggcagcggtatcatcaacaggcttacccgtcttactgtctactagagtccggcaaaaaaacgggcaaggtg
tcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttactagagtgccacctgacgtctaagaatac
tagagaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctac
gtaatactagagttagttagttagtactagagcccttagtgactctactagaggaattcgcggccgcttctagagtact
agagacgcgtggatccggcttactaaaagccagataacagtatgcgtatttgcgcgctgatttttgcggtataagaata
tatactgatatgtatacccgaagtatgtcaaaaagaggtatgctaatgcagtttaaggtttacacctataaaagagaga
gccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccag
tgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatg
accaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatca
aaaacgccattaacctgatgttctggggaatataaatgtcaggctcccttatacacagccagttactagagctgtcaga
ccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatccttttt
gataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggat
cttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgttt
gccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttcta
gtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccag
tggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtc
gggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgag
ctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagc
gcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcg
atttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttt
tgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgt