##gff-version 3 ##sequence-region pSB3T5 1 3252 pSB3T5 . sequence_feature 3206 3217 . + 0 ID=BBa_B0042;Name=BBa_B0042 pSB3T5 . stop_codon 3210 3212 . + 0 ID=annotation1797088;Name=stop codon (-3 frame);Parent=BBa_B0042 pSB3T5 . stop_codon 3215 3217 . + 0 ID=annotation1797086;Name=stop codon (+1 frame);Parent=BBa_B0042 pSB3T5 . stop_codon 3214 3216 . + 0 ID=annotation1797087;Name=stop codon (-2 frame);Parent=BBa_B0042 pSB3T5 . stop_codon 3211 3213 . + 0 ID=annotation1797085;Name=stop codon (+3 frame);Parent=BBa_B0042 pSB3T5 . stop_codon 3206 3208 . + 0 ID=annotation1797084;Name=stop codon (-1 frame);Parent=BBa_B0042 pSB3T5 . stop_codon 3207 3209 . + 0 ID=annotation1797083;Name=stop codon (+2 frame);Parent=BBa_B0042 pSB3T5 . terminator 3036 3107 . + 0 ID=BBa_B0053;Name=BBa_B0053 pSB3T5 . stem_loop 3044 3078 . + 0 ID=annotation318602;Name=stem_loop;Parent=BBa_B0053 pSB3T5 . sequence_feature 1 21 . + 0 ID=BBa_G00001;Name=BBa_G00001 pSB3T5 . sequence_feature 16 21 . + 0 ID=annotation1934227;Name=PstI;Parent=BBa_G00001 pSB3T5 . sequence_feature 9 16 . + 0 ID=annotation1934228;Name=NotI;Parent=BBa_G00001 pSB3T5 . sequence_feature 2 7 . + 0 ID=annotation1934226;Name=SpeI;Parent=BBa_G00001 pSB3T5 . engineered_region 1 21 . + 0 ID=annotation1821544;Name=Suffix;Parent=BBa_G00001 pSB3T5 . sequence_feature 3231 3252 . + 0 ID=BBa_G00000;Name=BBa_G00000 pSB3T5 . sequence_feature 3231 3236 . + 0 ID=annotation1934223;Name=EcoRI site;Parent=BBa_G00000 pSB3T5 . engineered_region 3231 3252 . + 0 ID=annotation1934225;Name=Prefix;Parent=BBa_G00000 pSB3T5 . sequence_feature 3246 3251 . + 0 ID=annotation1934224;Name=XbaI site;Parent=BBa_G00000 pSB3T5 . sequence_feature 3237 3244 . + 0 ID=annotation1961228;Name=NotI site;Parent=BBa_G00000 pSB3T5 . terminator 136 176 . + 0 ID=BBa_B0062;Name=BBa_B0062 pSB3T5 . stem_loop 149 168 . + 0 ID=annotation318637;Name=stem_loop;Parent=BBa_B0062 pSB3T5 . sequence_feature 3029 3035 . + 0 ID=BBa_G0004;Name=BBa_G0004 pSB3T5 . engineered_region 1746 3028 . + 0 ID=BBa_P1005;Name=BBa_P1005 pSB3T5 . CDS 1835 3028 . + 0 ID=annotation1897141;Name=tetA;Parent=BBa_P1005 pSB3T5 . promoter 1746 1834 . + 0 ID=annotation1897140;Name=tetR promoter;Parent=BBa_P1005 pSB3T5 . primer 3108 3127 . + 0 ID=BBa_G00100;Name=BBa_G00100 pSB3T5 . primer_binding_site 3108 3127 . + 0 ID=annotation1934505;Name=VF2;Parent=BBa_G00100 pSB3T5 . sequence_feature 184 1737 . + 0 ID=BBa_I50032;Name=BBa_I50032 pSB3T5 . sequence_feature 582 1406 . + 0 ID=annotation1928307;Name=p15A origin of replication;Parent=BBa_I50032 pSB3T5 . sequence_feature 1406 1633 . + 0 ID=annotation1928308;Name=repeat region;Parent=BBa_I50032 pSB3T5 . sequence_feature 3218 3230 . + 0 ID=BBa_B0043;Name=BBa_B0043 pSB3T5 . sequence_feature 3222 3222 . + 0 ID=annotation1797104;Name=Generated T overhang;Parent=BBa_B0043 pSB3T5 . sequence_feature 3218 3222 . + 0 ID=annotation1797101;Name=Topoisomerase I recognition site;Parent=BBa_B0043 pSB3T5 . sequence_feature 3226 3230 . + 0 ID=annotation1797102;Name=Nt.BstNB I recognition site;Parent=BBa_B0043 pSB3T5 . sequence_feature 3223 3225 . + 0 ID=annotation1797103;Name=Arbitrary sequence (could be any bases);Parent=BBa_B0043 pSB3T5 . sequence_feature 22 34 . + 0 ID=BBa_B0044;Name=BBa_B0044 pSB3T5 . sequence_feature 22 26 . + 0 ID=annotation1797126;Name=Nt.BstNB I recognition site;Parent=BBa_B0044 pSB3T5 . sequence_feature 30 30 . + 0 ID=annotation1797129;Name=Generated T overhang;Parent=BBa_B0044 pSB3T5 . sequence_feature 30 34 . + 0 ID=annotation1797128;Name=Topoisomerase I recognition site;Parent=BBa_B0044 pSB3T5 . sequence_feature 27 29 . + 0 ID=annotation1797127;Name=Arbitrary sequence (could be any bases);Parent=BBa_B0044 pSB3T5 . sequence_feature 116 135 . + 0 ID=BBa_G00102;Name=BBa_G00102 pSB3T5 . primer_binding_site 116 135 . + 0 ID=annotation1961229;Name=VR;Parent=BBa_G00102 pSB3T5 . sequence_feature 35 46 . + 0 ID=BBa_B0042;Name=BBa_B0042 pSB3T5 . stop_codon 39 41 . + 0 ID=annotation1797088;Name=stop codon (-3 frame);Parent=BBa_B0042 pSB3T5 . stop_codon 44 46 . + 0 ID=annotation1797086;Name=stop codon (+1 frame);Parent=BBa_B0042 pSB3T5 . stop_codon 43 45 . + 0 ID=annotation1797087;Name=stop codon (-2 frame);Parent=BBa_B0042 pSB3T5 . stop_codon 40 42 . + 0 ID=annotation1797085;Name=stop codon (+3 frame);Parent=BBa_B0042 pSB3T5 . stop_codon 35 37 . + 0 ID=annotation1797084;Name=stop codon (-1 frame);Parent=BBa_B0042 pSB3T5 . stop_codon 36 38 . + 0 ID=annotation1797083;Name=stop codon (+2 frame);Parent=BBa_B0042 pSB3T5 . terminator 3128 3205 . + 0 ID=BBa_B0055;Name=BBa_B0055 pSB3T5 . sequence_feature 3155 3193 . + 0 ID=annotation1783311;Name=identical to bacteriophage T3;Parent=BBa_B0055 pSB3T5 . stem_loop 3163 3184 . + 0 ID=annotation1783301;Name=stem loop from bacteriophage T3;Parent=BBa_B0055 pSB3T5 . sequence_feature 177 183 . + 0 ID=BBa_G0003;Name=BBa_G0003 pSB3T5 . sequence_feature 1738 1745 . + 0 ID=BBa_G0002;Name=BBa_G0002 pSB3T5 . terminator 47 115 . + 0 ID=BBa_B0054;Name=BBa_B0054 pSB3T5 . sequence_feature 50 89 . + 0 ID=annotation1783342;Name=identical to E. coli MG1655 between tonB and yciA;Parent=BBa_B0054 pSB3T5 . stem_loop 57 88 . + 0 ID=annotation1747359;Name=stem loop;Parent=BBa_B0054 >pSB3T5 tactagtagcggccgctgcaggagtcactaagggttagttagttagattagcagaaagtcaaaagcctccgaccggagg cttttgactaaaacttcccttggggttatcattggggctcactcaaaggcggtaatcagataaaaaaaatccttagctt tcgctaaggatgatttctgctagagatggaatagactggatggaggcggataaagttgcaggaccacttctgcgctcgg cccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggg gccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacag atcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatt taaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtga gttttcgttccactgagcgtcagaccccttaataagatgatcttcttgagatcgttttggtctgcgcgtaatctcttgc tctgaaaacgaaaaaaccgccttgcagggcggtttttcgaaggttctctgagctaccaactctttgaaccgaggtaact ggcttggaggagcgcagtcaccaaaacttgtcctttcagtttagccttaaccggcgcatgacttcaagactaactcctc taaatcaattaccagtggctgctgccagtggtgcttttgcatgtctttccgggttggactcaagacgatagttaccgga taaggcgcagcggtcggactgaacggggggttcgtgcatacagtccagcttggagcgaactgcctacccggaactgagt gtcaggcgtggaatgagacaaacgcggccataacagcggaatgacaccggtaaaccgaaaggcaggaacaggagagcgc acgagggagccgccaggggaaacgcctggtatctttatagtcctgtcgggtttcgccaccactgatttgagcgtcagat ttcgtgatgcttgtcaggggggcggagcctatggaaaaacggctttgccgcggccctctcacttccctgttaagtatct tcctggcatcttccaggaaatctccgccccgttcgtaagccatttccgctcgccgcagtcgaacgaccgagcgtagcga gtcagtgagcgaggaagcggaatatatcctgtatcacatattctgctgacgcaccggtgcagccttttttctcctgcca catgaagcacttcactgacaccctcatcagtgccaacatagtaagccagtatacactccgctagcgctgaggtctgcct cgtgaagaaggtgttgctgactcataccaggcctgaatcgccccatcatccagccagaaagtgagggagccacggttga tgagagctttgttgtaggtggaccagttggtgattttgaacttttgctttgccacggaacggtctgcgttgtcgggaag atgcgtgatctgatccttcaactcagcaaaagttcgatttattcaacaaagccacgttgtgtctcaaaatctctgatgt tacattgcacaagataaaaatatatcatcatgaacaataaaactgtctgcttacataaacagtaatacaaggggtgttt actagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaac gcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcat aggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtg ctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtc cagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctcta cgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggg gaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggac tgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgctt ccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgg gcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgc tctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatctt gcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatg gcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcg cttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttca agggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcg agcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcat ggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctt taaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaaga aaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcg gccgcttctagag