##gff-version 3 ##sequence-region pSB3K5 1 2936 pSB3K5 . sequence_feature 116 135 . + 0 ID=BBa_G00102;Name=BBa_G00102 pSB3K5 . primer_binding_site 116 135 . + 0 ID=annotation1961229;Name=VR;Parent=BBa_G00102 pSB3K5 . engineered_region 1746 2712 . + 0 ID=BBa_P1003;Name=BBa_P1003 pSB3K5 . CDS 1894 2712 . + 0 ID=annotation1897112;Name=kanamycin resistance;Parent=BBa_P1003 pSB3K5 . sequence_feature 22 34 . + 0 ID=BBa_B0044;Name=BBa_B0044 pSB3K5 . sequence_feature 30 34 . + 0 ID=annotation1797128;Name=Topoisomerase I recognition site;Parent=BBa_B0044 pSB3K5 . sequence_feature 22 26 . + 0 ID=annotation1797126;Name=Nt.BstNB I recognition site;Parent=BBa_B0044 pSB3K5 . sequence_feature 27 29 . + 0 ID=annotation1797127;Name=Arbitrary sequence (could be any bases);Parent=BBa_B0044 pSB3K5 . sequence_feature 30 30 . + 0 ID=annotation1797129;Name=Generated T overhang;Parent=BBa_B0044 pSB3K5 . sequence_feature 2902 2914 . + 0 ID=BBa_B0043;Name=BBa_B0043 pSB3K5 . sequence_feature 2906 2906 . + 0 ID=annotation1797104;Name=Generated T overhang;Parent=BBa_B0043 pSB3K5 . sequence_feature 2907 2909 . + 0 ID=annotation1797103;Name=Arbitrary sequence (could be any bases);Parent=BBa_B0043 pSB3K5 . sequence_feature 2902 2906 . + 0 ID=annotation1797101;Name=Topoisomerase I recognition site;Parent=BBa_B0043 pSB3K5 . sequence_feature 2910 2914 . + 0 ID=annotation1797102;Name=Nt.BstNB I recognition site;Parent=BBa_B0043 pSB3K5 . terminator 136 176 . + 0 ID=BBa_B0062;Name=BBa_B0062 pSB3K5 . stem_loop 149 168 . + 0 ID=annotation318637;Name=stem_loop;Parent=BBa_B0062 pSB3K5 . terminator 2812 2889 . + 0 ID=BBa_B0055;Name=BBa_B0055 pSB3K5 . stem_loop 2847 2868 . + 0 ID=annotation1783301;Name=stem loop from bacteriophage T3;Parent=BBa_B0055 pSB3K5 . sequence_feature 2839 2877 . + 0 ID=annotation1783311;Name=identical to bacteriophage T3;Parent=BBa_B0055 pSB3K5 . terminator 47 115 . + 0 ID=BBa_B0054;Name=BBa_B0054 pSB3K5 . sequence_feature 50 89 . + 0 ID=annotation1783342;Name=identical to E. coli MG1655 between tonB and yciA;Parent=BBa_B0054 pSB3K5 . stem_loop 57 88 . + 0 ID=annotation1747359;Name=stem loop;Parent=BBa_B0054 pSB3K5 . sequence_feature 2890 2901 . + 0 ID=BBa_B0042;Name=BBa_B0042 pSB3K5 . stop_codon 2890 2892 . + 0 ID=annotation1797084;Name=stop codon (-1 frame);Parent=BBa_B0042 pSB3K5 . stop_codon 2898 2900 . + 0 ID=annotation1797087;Name=stop codon (-2 frame);Parent=BBa_B0042 pSB3K5 . stop_codon 2899 2901 . + 0 ID=annotation1797086;Name=stop codon (+1 frame);Parent=BBa_B0042 pSB3K5 . stop_codon 2894 2896 . + 0 ID=annotation1797088;Name=stop codon (-3 frame);Parent=BBa_B0042 pSB3K5 . stop_codon 2891 2893 . + 0 ID=annotation1797083;Name=stop codon (+2 frame);Parent=BBa_B0042 pSB3K5 . stop_codon 2895 2897 . + 0 ID=annotation1797085;Name=stop codon (+3 frame);Parent=BBa_B0042 pSB3K5 . terminator 2720 2791 . + 0 ID=BBa_B0053;Name=BBa_B0053 pSB3K5 . stem_loop 2728 2762 . + 0 ID=annotation318602;Name=stem_loop;Parent=BBa_B0053 pSB3K5 . sequence_feature 177 183 . + 0 ID=BBa_G0003;Name=BBa_G0003 pSB3K5 . sequence_feature 1 21 . + 0 ID=BBa_G00001;Name=BBa_G00001 pSB3K5 . sequence_feature 2 7 . + 0 ID=annotation1934226;Name=SpeI;Parent=BBa_G00001 pSB3K5 . sequence_feature 16 21 . + 0 ID=annotation1934227;Name=PstI;Parent=BBa_G00001 pSB3K5 . engineered_region 1 21 . + 0 ID=annotation1821544;Name=Suffix;Parent=BBa_G00001 pSB3K5 . sequence_feature 9 16 . + 0 ID=annotation1934228;Name=NotI;Parent=BBa_G00001 pSB3K5 . sequence_feature 35 46 . + 0 ID=BBa_B0042;Name=BBa_B0042 pSB3K5 . stop_codon 35 37 . + 0 ID=annotation1797084;Name=stop codon (-1 frame);Parent=BBa_B0042 pSB3K5 . stop_codon 43 45 . + 0 ID=annotation1797087;Name=stop codon (-2 frame);Parent=BBa_B0042 pSB3K5 . stop_codon 44 46 . + 0 ID=annotation1797086;Name=stop codon (+1 frame);Parent=BBa_B0042 pSB3K5 . stop_codon 39 41 . + 0 ID=annotation1797088;Name=stop codon (-3 frame);Parent=BBa_B0042 pSB3K5 . stop_codon 36 38 . + 0 ID=annotation1797083;Name=stop codon (+2 frame);Parent=BBa_B0042 pSB3K5 . stop_codon 40 42 . + 0 ID=annotation1797085;Name=stop codon (+3 frame);Parent=BBa_B0042 pSB3K5 . sequence_feature 1738 1745 . + 0 ID=BBa_G0002;Name=BBa_G0002 pSB3K5 . primer 2792 2811 . + 0 ID=BBa_G00100;Name=BBa_G00100 pSB3K5 . primer_binding_site 2792 2811 . + 0 ID=annotation1934505;Name=VF2;Parent=BBa_G00100 pSB3K5 . sequence_feature 2713 2719 . + 0 ID=BBa_G0004;Name=BBa_G0004 pSB3K5 . sequence_feature 184 1737 . + 0 ID=BBa_I50032;Name=BBa_I50032 pSB3K5 . sequence_feature 1406 1633 . + 0 ID=annotation1928308;Name=repeat region;Parent=BBa_I50032 pSB3K5 . sequence_feature 582 1406 . + 0 ID=annotation1928307;Name=p15A origin of replication;Parent=BBa_I50032 pSB3K5 . sequence_feature 2915 2936 . + 0 ID=BBa_G00000;Name=BBa_G00000 pSB3K5 . sequence_feature 2930 2935 . + 0 ID=annotation1934224;Name=XbaI site;Parent=BBa_G00000 pSB3K5 . sequence_feature 2915 2920 . + 0 ID=annotation1934223;Name=EcoRI site;Parent=BBa_G00000 pSB3K5 . engineered_region 2915 2936 . + 0 ID=annotation1934225;Name=Prefix;Parent=BBa_G00000 pSB3K5 . sequence_feature 2921 2928 . + 0 ID=annotation1961228;Name=NotI site;Parent=BBa_G00000 >pSB3K5 tactagtagcggccgctgcaggagtcactaagggttagttagttagattagcagaaagtcaaaagcctccgaccggagg cttttgactaaaacttcccttggggttatcattggggctcactcaaaggcggtaatcagataaaaaaaatccttagctt tcgctaaggatgatttctgctagagatggaatagactggatggaggcggataaagttgcaggaccacttctgcgctcgg cccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggg gccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacag atcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatt taaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtga gttttcgttccactgagcgtcagaccccttaataagatgatcttcttgagatcgttttggtctgcgcgtaatctcttgc tctgaaaacgaaaaaaccgccttgcagggcggtttttcgaaggttctctgagctaccaactctttgaaccgaggtaact ggcttggaggagcgcagtcaccaaaacttgtcctttcagtttagccttaaccggcgcatgacttcaagactaactcctc taaatcaattaccagtggctgctgccagtggtgcttttgcatgtctttccgggttggactcaagacgatagttaccgga taaggcgcagcggtcggactgaacggggggttcgtgcatacagtccagcttggagcgaactgcctacccggaactgagt gtcaggcgtggaatgagacaaacgcggccataacagcggaatgacaccggtaaaccgaaaggcaggaacaggagagcgc acgagggagccgccaggggaaacgcctggtatctttatagtcctgtcgggtttcgccaccactgatttgagcgtcagat ttcgtgatgcttgtcaggggggcggagcctatggaaaaacggctttgccgcggccctctcacttccctgttaagtatct tcctggcatcttccaggaaatctccgccccgttcgtaagccatttccgctcgccgcagtcgaacgaccgagcgtagcga gtcagtgagcgaggaagcggaatatatcctgtatcacatattctgctgacgcaccggtgcagccttttttctcctgcca catgaagcacttcactgacaccctcatcagtgccaacatagtaagccagtatacactccgctagcgctgaggtctgcct cgtgaagaaggtgttgctgactcataccaggcctgaatcgccccatcatccagccagaaagtgagggagccacggttga tgagagctttgttgtaggtggaccagttggtgattttgaacttttgctttgccacggaacggtctgcgttgtcgggaag atgcgtgatctgatccttcaactcagcaaaagttcgatttattcaacaaagccacgttgtgtctcaaaatctctgatgt tacattgcacaagataaaaatatatcatcatgaacaataaaactgtctgcttacataaacagtaatacaaggggtgttt actagagctgatccttcaactcagcaaaagttcgatttattcaacaaagccacgttgtgtctcaaaatctctgatgtta cattgcacaagataaaaatatatcatcatgaacaataaaactgtctgcttacataaacagtaatacaaggggtgttatg agccatattcaacgggaaacgtcttgctcccgtccgcgcttaaactccaacatggacgctgatttatatgggtataaat gggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgcttgtatgggaagcccgatgcgccagagttgtttct gaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtccgtctcaactggctgacggagtttatgcct ctcccgaccatcaagcattttatccgtactcctgatgatgcgtggttactcaccaccgcgattcctgggaaaacagcct tccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggccgtgttcctgcgccggttacattc gattcctgtttgtaattgtccttttaacagcgatcgtgtatttcgtcttgctcaggcgcaatcacgcatgaataacggt ttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcacaagctct tgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaat aggttgtattgatgttggacgggtcggaatcgcagaccgttaccaggaccttgccattctttggaactgcctcggtgag ttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatt tgatgctcgatgagtttttctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctt taaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaaga aaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcg gccgcttctagag