##gff-version 3
##sequence-region pSB2K4 1 4467
pSB2K4	.	sequence_alteration	2774	2774	.	+	0	ID=annotation1974336;Name=T->C
pSB2K4	.	primer_binding_site	184	203	.	+	0	ID=annotation1974296;Name=Verification reverse
pSB2K4	.	promoter	1433	1441	.	+	0	ID=annotation1974316;Name=Possible -10
pSB2K4	.	promoter	1444	1452	.	+	0	ID=annotation1974317;Name=Possible -10
pSB2K4	.	sequence_feature	2565	2565	.	+	0	ID=annotation1974332;Name=Maybe A or G
pSB2K4	.	sequence_feature	1490	1706	.	+	0	ID=annotation1974328;Name=oriS
pSB2K4	.	sequence_alteration	1142	1142	.	+	0	ID=annotation1974313;Name=C->T
pSB2K4	.	sequence_feature	1447	1447	.	+	0	ID=annotation1974318;Name=Predicted transcription start
pSB2K4	.	stem_loop	59	91	.	+	0	ID=annotation1974293;Name=Stem loop
pSB2K4	.	sequence_alteration	857	857	.	+	0	ID=annotation1974309;Name=G->C
pSB2K4	.	point_mutation	3702	3702	.	+	0	ID=annotation1974346;Name=T->C
pSB2K4	.	stem_loop	4402	4421	.	+	0	ID=annotation1974354;Name=Stem loop
pSB2K4	.	primer_binding_site	4313	4332	.	+	0	ID=annotation1974351;Name=VF2 (not an exact match, but works)
pSB2K4	.	promoter	3375	3420	.	+	0	ID=annotation1974344;Name=nnpp promoter 1 (.92)'
pSB2K4	.	promoter	3269	3314	.	+	0	ID=annotation1974339;Name=nnpp promoter 3 (0.95)'
pSB2K4	.	sequence_feature	1636	1682	.	+	0	ID=annotation1974329;Name=Low GC region
pSB2K4	.	sequence_feature	1823	1844	.	+	0	ID=annotation1974330;Name=Low GC region
pSB2K4	.	sequence_feature	269	317	.	+	0	ID=annotation1974297;Name=f1 ori
pSB2K4	.	sequence_alteration	3017	3017	.	+	0	ID=annotation1974337;Name=G->A
pSB2K4	.	promoter	3379	3387	.	+	0	ID=annotation1974342;Name=Predicted -10
pSB2K4	.	operator	597	619	.	+	0	ID=annotation1974306;Name=repE binding site
pSB2K4	.	sequence_alteration	1091	1091	.	+	0	ID=annotation1974311;Name=G->C
pSB2K4	.	operator	1581	1591	.	+	0	ID=annotation1974327;Name=repE binding site
pSB2K4	.	point_mutation	3507	3507	.	+	0	ID=annotation1974345;Name=T->A
pSB2K4	.	CDS	644	1399	.	+	0	ID=annotation1974310;Name=repE
pSB2K4	.	promoter	1455	1463	.	+	0	ID=annotation1974320;Name=Possible -10
pSB2K4	.	promoter	1477	1482	.	+	0	ID=annotation1974323;Name=Possible -35
pSB2K4	.	stem_loop	51	120	.	+	0	ID=annotation1974292;Name=E. coli his operon terminator
pSB2K4	.	promoter	1426	1431	.	+	0	ID=annotation1974315;Name=Possible -35
pSB2K4	.	sequence_alteration	1100	1100	.	+	0	ID=annotation1974312;Name=T->C
pSB2K4	.	sequence_feature	3394	3394	.	+	0	ID=annotation1974343;Name=Predicted transcription start
pSB2K4	.	sequence_alteration	551	551	.	+	0	ID=annotation1974303;Name=T->A mutation
pSB2K4	.	CDS	3460	4275	.	+	0	ID=annotation1974348;Name=kanR
pSB2K4	.	point_mutation	4119	4119	.	+	0	ID=annotation1974350;Name=C->T
pSB2K4	.	sequence_alteration	2699	2699	.	+	0	ID=annotation1974334;Name=T->G
pSB2K4	.	operator	1527	1537	.	+	0	ID=annotation1974325;Name=repE binding site
pSB2K4	.	sequence_feature	1460	1460	.	+	0	ID=annotation1974322;Name=Predicted transcription start
pSB2K4	.	sequence_alteration	462	462	.	+	0	ID=annotation1974300;Name=A->G mutation
pSB2K4	.	sequence_feature	394	641	.	+	0	ID=annotation1974302;Name=incC
pSB2K4	.	promoter	1455	1460	.	+	0	ID=annotation1974321;Name=Possible -35
pSB2K4	.	operator	477	495	.	+	0	ID=annotation1974301;Name=repE binding site
pSB2K4	.	operator	1552	1570	.	+	0	ID=annotation1974326;Name=repE binding site
pSB2K4	.	promoter	2064	2238	.	+	0	ID=annotation1974331;Name=lacOP
pSB2K4	.	CDS	2316	3161	.	+	0	ID=annotation1974335;Name=repL
pSB2K4	.	promoter	3359	3364	.	+	0	ID=annotation1974340;Name=Predicted -35
pSB2K4	.	point_mutation	3903	3903	.	+	0	ID=annotation1974349;Name=T->G
pSB2K4	.	point_mutation	3849	3849	.	+	0	ID=annotation1974347;Name=G->C
pSB2K4	.	sequence_alteration	2567	2567	.	+	0	ID=annotation1974333;Name=T->G
pSB2K4	.	sequence_alteration	3251	3251	.	+	0	ID=annotation1974338;Name=T->C
pSB2K4	.	operator	568	586	.	+	0	ID=annotation1974304;Name=repE binding site
pSB2K4	.	sequence_alteration	692	692	.	+	0	ID=annotation1974308;Name=A->C
pSB2K4	.	operator	394	412	.	+	0	ID=annotation1974299;Name=repE binding site
pSB2K4	.	stem_loop	4389	4392	.	+	0	ID=annotation1974353;Name=end of terminator
pSB2K4	.	sequence_alteration	146	146	.	+	0	ID=annotation1974295;Name=C->A
pSB2K4	.	sequence_alteration	4321	4321	.	+	0	ID=annotation1974352;Name=G->C
pSB2K4	.	operator	623	641	.	+	0	ID=annotation1974307;Name=repE binding site
pSB2K4	.	sequence_feature	4446	4467	.	+	0	ID=annotation1974355;Name=BioBrick prefix
pSB2K4	.	operator	1497	1515	.	+	0	ID=annotation1974324;Name=repE binding site
pSB2K4	.	sequence_alteration	1454	1454	.	+	0	ID=annotation1974319;Name=G->T
pSB2K4	.	sequence_alteration	292	292	.	+	0	ID=annotation1974298;Name=T->A
pSB2K4	.	sequence_feature	1426	1426	.	+	0	ID=annotation1974314;Name=Predicted transcription start
pSB2K4	.	sequence_feature	1	21	.	+	0	ID=annotation1974291;Name=BioBrick suffix
pSB2K4	.	promoter	3353	3398	.	+	0	ID=annotation1974341;Name=nnpp promoter 2 (0.96)'
pSB2K4	.	sequence_alteration	93	93	.	+	0	ID=annotation1974294;Name=T->C
pSB2K4	.	sequence_alteration	578	578	.	+	0	ID=annotation1974305;Name=T->C
>pSB2K4
tactagtagcggccgctgcagcaccggtggcagttcgaatgatcatgcagtccggcaaaaaagggcaaggtgtcaccac
cctgccctttttccttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactagctgcgctcggt
cgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatggcgcggctcgcgtacccataatacccataatag
ctgtttgccatcgcgtatgcatcgatcacgtgtccacgttctttaatagtggacacttgttccaaactggaacaacact
cggatcgaaccgccgcgcaccgtgggaaaaactccaggtagaggtacacacgcggatagccaattcagagtaataaact
gtgataatcaaccctcatcaatgatgacgaactaacccccgatatcaggtcacatgacgaagggaaggagaaggaaatc
aactgtgacaaactgccctcaaatttggcttccttaaaaattacagttcaaaaagtatgagaaaatccatgcaggcaga
aggaaacagcaaaactgtgacaaactaccctcagtaggtcagaacaaatgtgacgaaccaccctcaaatctgtgacaga
taaccctcagactatcctgtcgtcatggaagtgatatcgcggaaggaaaatacgatatgcgtcgtctggcggcctttct
ttttctcaatgtatgagaggcgcattggagttctgctgttgatctcattaacacagacttgcaggaagcggcggcggaa
gtcaggcatacgctggtaactttgaggcagctggtaacgctctatgatccagtcgattttcagagacacgatgcctgag
ccatccggcttacgatactgacacagggattcgtataaacgcatggcatacggattggtgatttcttttgtttcactaa
gccgaaactgcgtaaaccgattctgtaacccgataaagaagggaatgagatatgggttgatatgtacactgtaaagccc
tctggatggactgtgcgcacgtttgataaaccaaggaaaagattcatagcctttttcatcacccgcatcctcctcaggg
cgataaaaaaccacttccttccccgcgaaactctttaatgcctgccgtatatccttactggcttccgcagaggtcaatc
cgaatatttcagcatatttagcaacatggatctcgcagataccgtcatgttcctgtagggtgccatcagattttctgat
ctggtcaacgaacagatacagcatacgtttttgatcccgagagagactatatgccgcctcagtgaggtcgtttgactgg
acgattcgcgggctatttttacgtttcttgtgattgataaccgctgtttccgccatgacagatccatgtgaagtgtgac
aagtttttagattgtcacactaaataaaaaatagtcaataagcagggataactttgtgaaaaaacagcttcttctgagg
gcaatttgtcacagggttaagggcaatttgtcacagacaggactgtcatttgagggtgatttgtcacactgaaagggca
atttgtcacaacaccttctgtagaaccagcatggataaaggcctacaaggcgctctaaaaaagaagatctaaaaactat
aaaaaaaataattataaaaatatccccgtggataagtggataaccccaagggaagttttttcaggcatcgtgtgtaagc
agaatatataagtgctgttccctggtgcttcctcgctcactcgaaattgccggggatagctttatgcttgtaaaccgtt
ttgtgaaaaaatttttaaaataaaaaaggggacctctagggtccccaattaattagtaatataatctattaaaggtcat
tcaaaaggtcatccaccggatcaattcccctgctcgcgcaggctgggtgccaagctctcgggtaacatcaaggcccgat
ccttggagcccttgccctcccgcacgatgatcgtgccgtgatcgaaatccagatccttgacccgcagttgcaaaccctc
actgatccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatg
tgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcgg
ataacaatttcacacaggaaacagctatgaccatgattacgccaagcgcggtacctgtcgcggcaacgcgctaacagac
gtagtaagaaccaccagcattgtaatgctggctaaagtcactttcctgagctgtataacgatgagcgattttacttttt
ctggctatgaattggcctgctttgtaacacactccggtctatcccgtagcgccgggcatatcctgtcgcaatgtgcaaa
tctcgcggcaacaaccagtgaatacttcattcacaagcctcaccgcctgatcgcggcagaaactggttatagccaatca
accgtcgttcgtgcattccgtgaagctgtaaacaaagggatcctgtctgtagagattgttatcggcgatcaccgtgaac
gtcgcgctaacctgtatcggtttacaccatcctttttggccttcgcacaacaagccaaaaatgcgctgatagaaagcaa
attaaagatctcgtcagcggcaaccaaggttaaagctgttctcgctaagacattggctttatttaattttttatccaca
cccccatgccaaaatgataccccctccccctgtcaggatgacgtggcaataaagaataagaagtcacaagttaaaaaaa
caaaaagatcagtttccggcggtgccggaacaaccagcctcaaaaaattgacttcatggatcgctaaggcaaaagcaaa
ggctgacaatctgcggttatccaaaaaacgcactcaaaaacatgagttcaagcagaaagtagaggcggctgcgcggaaa
tatgcttacctgaaaaacaagcgttcgcctgatattggcgggatatcaaacttcgataacctaccgcattgcatgacgg
taaacgaagctcttaatgcggttttagccaaaaataaagataacgaacaatggggtataccggcaggattcagagggta
atgaattgctctaattataaccatgcatactttcaacacctctagtttgccatgaggcaaactcataggtgtcctggta
agaggacactgctgccaaaactggacgccccattattgcaattaataaacaactaacggacaattctacctaacaataa
gtggagttgcggcccgccgcgatccgctaggaaagccacgttgtgtctcaaaatctctgatgttacattgcacaagata
aaaatatatcatcatgaacaataaaactgtctgcttacataaacagtaatacaaggggtgttatgagccatattcaacg
ggaaacgtcttgctcaaggccgcgattaaaatccaacatggatgctgatttatatgggtataaatgggctcgcgataat
gtcgggcaatcaggtgcgacaatctaccgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaag
gtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctctcccgaccatcaa
gcattttatccgtactcctgatgatgcatggttactcaccactgcgatccccggaaaaacagcattccaggtattagaa
gaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccgcttgcattcgattcctgtttgta
attgtccttttaacagcgatcgcgtatttcggctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgag
tgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcataaactgttgccattctcaccg
gattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatg
ttggacgagttggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggtgagttttctccttcatt
acagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgag
tttttctaatcagaattggttaattggttgtaacactggcgagctgtgccacctcacgtctaagaaaccattattatca
tgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatga
tttctggtattctgcggaccggaattcgcggccgcttctagag