##gff-version 3
##sequence-region pSB1AC3 1 3055
pSB1AC3	.	stem_loop	30	64	.	+	0	ID=annotation1779665;Name=terminator stem loop
pSB1AC3	.	engineered_region	1	21	.	+	0	ID=annotation1779617;Name=Suffix
pSB1AC3	.	stem_loop	3005	3024	.	+	0	ID=annotation1779720;Name=terminator stem loop
pSB1AC3	.	stem_loop	1042	1147	.	+	0	ID=annotation1782172;Name=T0 terminator
pSB1AC3	.	CDS	1163	1821	.	+	0	ID=annotation1779657;Name=Cm R (rev)
pSB1AC3	.	CDS	261	875	.	+	0	ID=annotation1779628;Name=rep (pMB1) (rev)
pSB1AC3	.	sequence_feature	22	93	.	+	0	ID=annotation1823948;Name=E. coli his operon terminator
pSB1AC3	.	sequence_feature	276	276	.	+	0	ID=annotation1779653;Name=ORI
pSB1AC3	.	primer_binding_site	157	176	.	+	0	ID=annotation1779731;Name=VR
pSB1AC3	.	primer_binding_site	2916	2935	.	+	0	ID=annotation1779730;Name=VF2
pSB1AC3	.	sequence_feature	34	35	.	+	0	ID=annotation1823815;Name=deletion of two bases
pSB1AC3	.	point_mutation	1237	1237	.	+	0	ID=annotation1782173;Name=Silent g->a mutation found during sequencing
pSB1AC3	.	engineered_region	3034	3055	.	+	0	ID=annotation1779618;Name=Prefix
pSB1AC3	.	sequence_feature	2992	2995	.	+	0	ID=annotation1823949;Name=end of terminator
pSB1AC3	.	CDS	1933	2793	.	+	0	ID=annotation1779661;Name=APr (rev)
>pSB1AC3
tactagtagcggccgctgcagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaa
gattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagc
tcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaa
aaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatc
gacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcg
ctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagc
tcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccg
accgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccac
tggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacact
agaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggca
aacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaaga
tcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatca
aaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggt
ctgacagctcgaggcttggattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggag
ttctgaggtcattactggatctatcaacaggagtccaagcgagctcgatatcaaattacgccccgccctgccactcatc
gcagtactgttgtaattcattaagcattctgccgacatggaagccatcacaaacggcatgatgaacctgaatcgccagc
ggcatcagcaccttgtcgccttgcgtataatatttgcccatggtgaaaacgggggcgaagaagttgtccatattggcca
cgtttaaatcaaaactggtgaaactcacccagggattggctgagacgaaaaacatattctcaataaaccctttagggaa
ataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattca
ctccagagcgatgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagct
caccgtctttcattgccatacgaaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccggataaaa
cttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagca
actgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatccagtgatttttttct
ccattttagcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtg
aaagttggaacctcttacgtgcccgatcaactcgagttaccaatgcttaatcagtgaggcacctatctcagcgatctgt
ctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggcccca
gtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccga
gcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcg
ccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcat
tcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcc
tccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtc
atgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccga
gttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacg
ttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactga
tcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataa
gggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcat
gagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacct
gacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaa
aatccttagctttcgctaaggatgatttctggaattcgcggccgcttctagag