##gff-version 3
##sequence-region pSB1A7 1 2431
pSB1A7	.	engineered_region	24	152	.	+	0	ID=annotation1903644;Name=B0015 (rev)
pSB1A7	.	sequence_alteration	2278	2278	.	+	0	ID=annotation1903657;Name=c > g
pSB1A7	.	sequence_feature	2273	2278	.	+	0	ID=annotation1903656;Name=mutated EcoRI
pSB1A7	.	sequence_feature	153	158	.	+	0	ID=annotation1903648;Name=mutated PstI
pSB1A7	.	stem_loop	2290	2333	.	+	0	ID=annotation1903659;Name=stem_loop
pSB1A7	.	sequence_feature	2231	2234	.	+	0	ID=annotation1903654;Name=end of terminator
pSB1A7	.	stem_loop	2374	2393	.	+	0	ID=annotation1903660;Name=stem_loop
pSB1A7	.	stem_loop	2244	2263	.	+	0	ID=annotation1903655;Name=stem_loop
pSB1A7	.	stem_loop	38	57	.	+	0	ID=annotation1903645;Name=stem_loop
pSB1A7	.	engineered_region	1	20	.	+	0	ID=annotation1903642;Name=suffix
pSB1A7	.	stem_loop	167	201	.	+	0	ID=annotation1903650;Name=stem_loop
pSB1A7	.	CDS	398	1012	.	+	0	ID=annotation1903652;Name=rep(pMB1) (rev)
pSB1A7	.	engineered_region	2279	2407	.	+	0	ID=annotation1903658;Name=B0015
pSB1A7	.	sequence_alteration	153	153	.	+	0	ID=annotation1903647;Name=c > g
pSB1A7	.	stem_loop	98	141	.	+	0	ID=annotation1903646;Name=stem_loop
pSB1A7	.	engineered_region	2411	2431	.	+	0	ID=annotation1903661;Name=prefix
pSB1A7	.	sequence_feature	159	229	.	+	0	ID=annotation1903649;Name=E. coli his operon terminator
pSB1A7	.	primer_binding_site	2155	2174	.	+	0	ID=annotation1903643;Name=VF2
pSB1A7	.	CDS	1172	2032	.	+	0	ID=annotation1903653;Name=ampR (rev)
pSB1A7	.	primer_binding_site	294	313	.	+	0	ID=annotation1903651;Name=VR
>pSB1A7
actagtagcggccgctgcaggcttataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcg
ttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctgggtgcag
tccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcagg
cttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacg
gttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaag
gccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggc
gaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgcc
gcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagt
tcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggta
actatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagc
gaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatc
tgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcg
gtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggg
gtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatc
cttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaa
tcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactac
gatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatca
gcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaatt
gttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggt
gtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttg
tgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggtta
tggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtc
attctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcaga
actttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagtt
cgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacagg
aaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattat
tgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttc
cgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcg
tatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattgccaggcatcaaat
aaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcac
actggctcaccttcgggtgggcctttctgcgtttatagcagaattcgcggccgcttctaga