##gff-version 3
##sequence-region pSB1A10 1 5263
pSB1A10	.	sequence_feature	22	37	.	+	0	ID=annotation1871305;Name=spacer artifact from 13450
pSB1A10	.	sequence_feature	5203	5223	.	+	0	ID=annotation1871318;Name=RNAse E site
pSB1A10	.	stem_loop	59	93	.	+	0	ID=annotation1871307;Name=Hairpin
pSB1A10	.	sequence_feature	963	1044	.	+	0	ID=annotation1871312;Name=Scar from BB suffix removal by I13450
pSB1A10	.	ribosome_entry_site	102	113	.	+	0	ID=annotation1871308;Name=B0034
pSB1A10	.	sequence_feature	38	58	.	+	0	ID=annotation1871306;Name=RNAse E site
pSB1A10	.	operator	3239	4448	.	+	0	ID=annotation1871315;Name=I0500 (pBAD/araC)
pSB1A10	.	sequence_feature	5224	5241	.	+	0	ID=annotation1871319;Name=spacer artifact from 13450
pSB1A10	.	ribosome_entry_site	4457	4468	.	+	0	ID=annotation1871316;Name=B0034
pSB1A10	.	engineered_region	5242	5263	.	+	0	ID=annotation1871320;Name=prefix
pSB1A10	.	engineered_region	1	21	.	+	0	ID=annotation1871283;Name=suffix
pSB1A10	.	sequence_feature	3158	3238	.	+	0	ID=annotation1871314;Name=Scar from BB prefix removal by I13450
pSB1A10	.	engineered_tag	801	825	.	+	0	ID=annotation1871310;Name=barcode
pSB1A10	.	sequence_feature	1045	3157	.	+	0	ID=annotation1871313;Name=psb1a3
pSB1A10	.	sequence_feature	834	962	.	+	0	ID=annotation1871311;Name=B0015 (Term)
pSB1A10	.	CDS	4475	5194	.	+	0	ID=annotation1871317;Name=E0040 (EGFP)
pSB1A10	.	CDS	120	800	.	+	0	ID=annotation1871309;Name=E1010 (mRFP1)
>pSB1A10
tactagtagcggccgctgcagcgatccattgcagtggtattatttgtattgatctccttactatctctcgagagattag
tacctttggagatctactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgt
ttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaag
gtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttcca
gtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaa
tgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttca
tctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagc
ttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggt
cactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatca
aactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccgg
tgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaag
actgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcc
tttctgcgtttatatactagagtcaattgaggtagaggtacacacgcgaactccgatagccaattcagagtaataaact
gtgataatcaatgcagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagatta
cttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcact
caaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggc
caggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgc
tcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctc
ctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacg
ctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgc
tgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggta
acaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaag
gacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaa
accaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctt
tgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaag
gatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgac
agttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccg
tcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcacc
ggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctcc
atccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattg
ctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttac
atgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtg
ttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtg
agtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataatac
cgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccg
ctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctg
ggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactctt
cctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaat
aaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaa
cctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaa
ttgaggtagaggtacacacgcgaactccgatagccaattcagagtaataaactgtgataatcaatgcatgtactagagt
tatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcatt
ttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggt
gctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaa
agatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgat
cgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccg
cagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgc
ccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagt
taagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgacc
gtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcacc
accccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataac
cgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgc
ttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgc
gtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaag
ccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacact
ttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttct
ccatacccgtttttttgggctagctactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactgg
agttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgat
gcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcacta
ctttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcc
cgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaa
ggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattgg
aatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattag
acacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcctt
ttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttc
ttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagtattatttgtat
tgatctggtgaattaggtcttcgaatcgaattcgcggccgcttctagag