//function/sensor/metal
type: Collection
persistent identity: function sensor metal
display id: function_sensor_metal
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: Fur box
member: BBa_K1758354
member: BBa_K346002
member: BBa_K540001
member: BBa_K824009
member: metalP
member: BBa_K1758333
member: PrFe
member: zinTp
member: BBa_K1755302
member: BBa_K1980006
member: BBa_K1980011
member: BBa_K1758314
member: BBa_K1758343
member: BBa_K1758344
member: BBa_K1460010
member: BBa_K1980010
member: BBa_K1755305
member: BBa_K1758331
member: BBa_K1163110
member: BBa_K1509001
member: BBa_K1163107
member: BBa_K1758342
member: BBa_K1758313
member: BBa_K1758312
member: BBa_K1980004
member: BBa_K1758325
member: BBa_K824012
member: pFe
member: BBa_K1980005
member: BBa_K1749000
member: BBa_K1755303
member: BBa_I721001
member: BBa_K1980012
member: BBa_K1758353
member: BBa_I760005
member: BBa_K1758332
member: BBa_K174016
member: BBa_K1758323
member: BBa_I731004
member: BBa_K1755301
member: BBa_K1758322
member: BBa_K1724000
member: BBa_K1980007
member: BBa_K1163101
member: BBa_K346054
member: BBa_K1758352
member: BBa_K1758335
member: BBa_K174017
member: BBa_K1758334
member: BBa_K1980008
member: BBa_K1460009
member: BBa_K824008
member: BBa_K1758351
member: BBa_K1980009
member: BBa_K1758321

BBa_I721001
type: ComponentDefinition
persistent identity: BBa I721001
display id: BBa_I721001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lead Promoter
creator: Jeffrey Hofmann
created: 2007-10-25T11:00:00Z
modified: 2015-08-31T04:07:53Z
discontinued: false
dominant: false
experience: experience/None
group u list: _113_
m user id: 0
owner id: 1531
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Lead Promoter (Part 4) messed up EcoRI site
mutable notes: none
mutable provenance: ralstonia metallidurans
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I721001_sequence/1

BBa_K540001
type: ComponentDefinition
persistent identity: BBa K540001
display id: BBa_K540001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rcn, cobalt-sensitive promoter
creator: Mathilde Dumond
created: 2011-09-14T11:00:00Z
modified: 2015-07-14T12:34:23Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _706_
m user id: 4206
owner id: 8419
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Rcn is a promoter that is activated by cobalt. A repressor is fixed to the promoter by default, and cobalt binds to this repressor, which activates the gene under control of this promoter. Rcn is also activated by nickel, but we have not characterized this aspect.
mutable notes: An internal iGEM restriction site had to be mutated by QuickChange mutagenesis.
mutable provenance: PCR from E.coli MC4100 genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: RcnR
sequence: https://synbiohub.org/public/igem/BBa_K540001_sequence/1

RcnR
type: SequenceAnnotation
persistent identity: annotation2129035
display id: annotation2129035
version: 1
top level: BBa_K540001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K540001/annotation2129035/range2129035/1

https://synbiohub.org/public/igem/BBa_K540001/annotation2129035/range2129035/1
type: Range
persistent identity: range2129035
display id: range2129035
version: 1
direction: direction/reverse
top level: BBa_K540001
start: 1
end: 279
orientation: inline

BBa_K1758312
type: ComponentDefinition
persistent identity: BBa K1758312
display id: BBa_K1758312
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Chromium responsive promoter with UTR+RBS and sfGFP
creator: Team Bielefeld-CeBiTec 2015
created: 2015-08-28T11:00:00Z
modified: 2016-01-06T03:35:44Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2180_
m user id: 4206
owner id: 26630
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Chromium responsive promoter with 5?? untremslated region and sfGFP for detection
mutable notes: ...
mutable provenance: Biobrick modified after K1058007
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: sfGFP
sequence annotation: 5-UTR
sequence annotation: T1
sequence annotation: T7-term
sequence annotation: Cr-promoter
sequence: https://synbiohub.org/public/igem/BBa_K1758312_sequence/1

sfGFP
type: SequenceAnnotation
persistent identity: annotation2439958
display id: annotation2439958
version: 1
top level: BBa_K1758312
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1758312/annotation2439958/range2439958/1

https://synbiohub.org/public/igem/BBa_K1758312/annotation2439958/range2439958/1
type: Range
persistent identity: range2439958
display id: range2439958
version: 1
direction: direction/forward
top level: BBa_K1758312
start: 217
end: 936
orientation: inline

5-UTR
type: SequenceAnnotation
persistent identity: annotation2439957
display id: annotation2439957
version: 1
top level: BBa_K1758312
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1758312/annotation2439957/range2439957/1

https://synbiohub.org/public/igem/BBa_K1758312/annotation2439957/range2439957/1
type: Range
persistent identity: range2439957
display id: range2439957
version: 1
direction: direction/forward
top level: BBa_K1758312
start: 171
end: 216
orientation: inline

T1
type: SequenceAnnotation
persistent identity: annotation2439959
display id: annotation2439959
version: 1
top level: BBa_K1758312
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1758312/annotation2439959/range2439959/1

https://synbiohub.org/public/igem/BBa_K1758312/annotation2439959/range2439959/1
type: Range
persistent identity: range2439959
display id: range2439959
version: 1
direction: direction/forward
top level: BBa_K1758312
start: 945
end: 1024
orientation: inline

T7-term
type: SequenceAnnotation
persistent identity: annotation2439960
display id: annotation2439960
version: 1
top level: BBa_K1758312
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1758312/annotation2439960/range2439960/1

https://synbiohub.org/public/igem/BBa_K1758312/annotation2439960/range2439960/1
type: Range
persistent identity: range2439960
display id: range2439960
version: 1
direction: direction/forward
top level: BBa_K1758312
start: 1033
end: 1073
orientation: inline

Cr-promoter
type: SequenceAnnotation
persistent identity: annotation2439956
display id: annotation2439956
version: 1
top level: BBa_K1758312
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1758312/annotation2439956/range2439956/1

https://synbiohub.org/public/igem/BBa_K1758312/annotation2439956/range2439956/1
type: Range
persistent identity: range2439956
display id: range2439956
version: 1
direction: direction/forward
top level: BBa_K1758312
start: 1
end: 164
orientation: inline

BBa_K824008
type: ComponentDefinition
persistent identity: BBa K824008
display id: BBa_K824008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cadmium Promoter with GFP Reporter
creator: Steven Allen
created: 2012-09-30T11:00:00Z
modified: 2015-07-24T01:19:31Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _786_1082_
m user id: 4206
owner id: 9218
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: We digested the promoter responsive to Cadmium (BBa_K174015) with EcoRI and SpeI and digested the GFP plasmid (BBa_I13401) with XbaI and PstI. The promoter was Chloramphenicol resistant and the GFP was Ampicillin resistant. These digested fragments were mixed and ligated to the provided, linearized pSB3C1 plasmid. The ligation mix was grown under Chloramphenicol selection. The resulting colonies were tested for responsive GFP production from the addition of Cadmium and Arsenic, respectively.
mutable notes: none
mutable provenance: This part is a combination of 2007 Brown's iGEM part BBa_K174015 and MIT's part (from 2005) I13401.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I13401
component: BBa_K174015
sequence annotation: BBa_I13401
sequence annotation: BBa_K174015
sequence: https://synbiohub.org/public/igem/BBa_K824008_sequence/1

BBa_I13401
type: Component
persistent identity: component2205262
display id: component2205262
version: 1
top level: BBa_K824008
access: public
definition: https://synbiohub.org/public/igem/BBa_I13401/1

BBa_K174015
type: Component
persistent identity: component2205254
display id: component2205254
version: 1
top level: BBa_K824008
access: public
definition: https://synbiohub.org/public/igem/BBa_K174015/1

BBa_I13401
type: SequenceAnnotation
persistent identity: annotation2205262
display id: annotation2205262
version: 1
top level: BBa_K824008
location: https://synbiohub.org/public/igem/BBa_K824008/annotation2205262/range2205262/1
component: BBa_I13401

https://synbiohub.org/public/igem/BBa_K824008/annotation2205262/range2205262/1
type: Range
persistent identity: range2205262
display id: range2205262
version: 1
direction: direction/reverse
top level: BBa_K824008
start: 212
end: 1068
orientation: inline

BBa_K174015
type: SequenceAnnotation
persistent identity: annotation2205254
display id: annotation2205254
version: 1
top level: BBa_K824008
location: https://synbiohub.org/public/igem/BBa_K824008/annotation2205254/range2205254/1
component: BBa_K174015

https://synbiohub.org/public/igem/BBa_K824008/annotation2205254/range2205254/1
type: Range
persistent identity: range2205254
display id: range2205254
version: 1
direction: direction/reverse
top level: BBa_K824008
start: 1
end: 205
orientation: inline

BBa_K1980011
type: ComponentDefinition
persistent identity: BBa K1980011
display id: BBa_K1980011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCopA MymT with divergent expressed CueR
creator: Sam Garforth
created: 2016-10-10T11:00:00Z
modified: 2016-10-24T03:08:06Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _2447_
m user id: 29607
owner id: 29607
owning group id: 9
status: status/Planning
bookmark: false
mutable description: pCopA MymT with plasmid constitutively expressed CueR
mutable notes: stuff
mutable provenance: E. coli TB
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K1980002
component: BBa_K1980006
sequence annotation: BBa_K1980002
sequence annotation: BBa_K1980006
sequence: https://synbiohub.org/public/igem/BBa_K1980011_sequence/1

BBa_K1980002
type: Component
persistent identity: component2493044
display id: component2493044
version: 1
top level: BBa_K1980011
access: public
definition: https://synbiohub.org/public/igem/BBa_K1980002/1

BBa_K1980006
type: Component
persistent identity: component2493043
display id: component2493043
version: 1
top level: BBa_K1980011
access: public
definition: BBa_K1980006

BBa_K1980002
type: SequenceAnnotation
persistent identity: annotation2493044
display id: annotation2493044
version: 1
top level: BBa_K1980011
location: https://synbiohub.org/public/igem/BBa_K1980011/annotation2493044/range2493044/1
component: BBa_K1980002

https://synbiohub.org/public/igem/BBa_K1980011/annotation2493044/range2493044/1
type: Range
persistent identity: range2493044
display id: range2493044
version: 1
direction: direction/reverse
top level: BBa_K1980011
start: 581
end: 763
orientation: inline

BBa_K1980006
type: SequenceAnnotation
persistent identity: annotation2493043
display id: annotation2493043
version: 1
top level: BBa_K1980011
location: https://synbiohub.org/public/igem/BBa_K1980011/annotation2493043/range2493043/1
component: BBa_K1980006

https://synbiohub.org/public/igem/BBa_K1980011/annotation2493043/range2493043/1
type: Range
persistent identity: range2493043
display id: range2493043
version: 1
direction: direction/reverse
top level: BBa_K1980011
start: 1
end: 574
orientation: inline

BBa_K1980007
type: ComponentDefinition
persistent identity: BBa K1980007
display id: BBa_K1980007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCusC mKate2
creator: Sam Garforth
created: 2016-10-10T11:00:00Z
modified: 2016-10-23T03:43:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2447_
m user id: 29607
owner id: 29607
owning group id: 9
status: status/Planning
bookmark: false
mutable description: pCusC mKate2. copper sensitive promoter with RFP reporter gene
mutable notes: Codon optimised.
mutable provenance: E. coli mainly
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: pCusC
sequence annotation: CusR binding
sequence annotation: Stop
sequence annotation: -35
sequence annotation: -10
sequence annotation: RBS
sequence annotation: mKate2
sequence: https://synbiohub.org/public/igem/BBa_K1980007_sequence/1

pCusC
type: SequenceAnnotation
persistent identity: annotation2525389
display id: annotation2525389
version: 1
top level: BBa_K1980007
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1980007/annotation2525389/range2525389/1

https://synbiohub.org/public/igem/BBa_K1980007/annotation2525389/range2525389/1
type: Range
persistent identity: range2525389
display id: range2525389
version: 1
direction: direction/forward
top level: BBa_K1980007
start: 1
end: 98
orientation: inline

CusR binding
type: SequenceAnnotation
persistent identity: annotation2528662
display id: annotation2528662
version: 1
top level: BBa_K1980007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1980007/annotation2528662/range2528662/1

https://synbiohub.org/public/igem/BBa_K1980007/annotation2528662/range2528662/1
type: Range
persistent identity: range2528662
display id: range2528662
version: 1
direction: direction/forward
top level: BBa_K1980007
start: 30
end: 51
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2528248
display id: annotation2528248
version: 1
top level: BBa_K1980007
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1980007/annotation2528248/range2528248/1

https://synbiohub.org/public/igem/BBa_K1980007/annotation2528248/range2528248/1
type: Range
persistent identity: range2528248
display id: range2528248
version: 1
direction: direction/forward
top level: BBa_K1980007
start: 819
end: 824
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2528244
display id: annotation2528244
version: 1
top level: BBa_K1980007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1980007/annotation2528244/range2528244/1

https://synbiohub.org/public/igem/BBa_K1980007/annotation2528244/range2528244/1
type: Range
persistent identity: range2528244
display id: range2528244
version: 1
direction: direction/forward
top level: BBa_K1980007
start: 60
end: 65
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2528245
display id: annotation2528245
version: 1
top level: BBa_K1980007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1980007/annotation2528245/range2528245/1

https://synbiohub.org/public/igem/BBa_K1980007/annotation2528245/range2528245/1
type: Range
persistent identity: range2528245
display id: range2528245
version: 1
direction: direction/forward
top level: BBa_K1980007
start: 81
end: 87
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2528246
display id: annotation2528246
version: 1
top level: BBa_K1980007
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1980007/annotation2528246/range2528246/1

https://synbiohub.org/public/igem/BBa_K1980007/annotation2528246/range2528246/1
type: Range
persistent identity: range2528246
display id: range2528246
version: 1
direction: direction/forward
top level: BBa_K1980007
start: 111
end: 116
orientation: inline

mKate2
type: SequenceAnnotation
persistent identity: annotation2528247
display id: annotation2528247
version: 1
top level: BBa_K1980007
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1980007/annotation2528247/range2528247/1

https://synbiohub.org/public/igem/BBa_K1980007/annotation2528247/range2528247/1
type: Range
persistent identity: range2528247
display id: range2528247
version: 1
direction: direction/forward
top level: BBa_K1980007
start: 126
end: 818
orientation: inline

BBa_K1980008
type: ComponentDefinition
persistent identity: BBa K1980008
display id: BBa_K1980008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCopA CueR sfGFP/ feedback pCopA sfGFP
creator: Sam Garforth
created: 2016-10-10T11:00:00Z
modified: 2016-10-24T07:34:54Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _2447_
m user id: 29607
owner id: 29607
owning group id: 9
status: status/Planning
bookmark: false
mutable description: pCopA CueR sfGFP/ feedback pCopA sfGFP
mutable notes: codon op
mutable provenance: E. coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: RBS
sequence annotation: Stop
sequence annotation: pCopA
sequence annotation: Hexahistidine
sequence annotation: sfGFP
sequence annotation: CueR
sequence: https://synbiohub.org/public/igem/BBa_K1980008_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2528250
display id: annotation2528250
version: 1
top level: BBa_K1980008
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1980008/annotation2528250/range2528250/1

https://synbiohub.org/public/igem/BBa_K1980008/annotation2528250/range2528250/1
type: Range
persistent identity: range2528250
display id: range2528250
version: 1
direction: direction/forward
top level: BBa_K1980008
start: 58
end: 69
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2528253
display id: annotation2528253
version: 1
top level: BBa_K1980008
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1980008/annotation2528253/range2528253/1

https://synbiohub.org/public/igem/BBa_K1980008/annotation2528253/range2528253/1
type: Range
persistent identity: range2528253
display id: range2528253
version: 1
direction: direction/forward
top level: BBa_K1980008
start: 499
end: 504
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2528256
display id: annotation2528256
version: 1
top level: BBa_K1980008
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1980008/annotation2528256/range2528256/1

https://synbiohub.org/public/igem/BBa_K1980008/annotation2528256/range2528256/1
type: Range
persistent identity: range2528256
display id: range2528256
version: 1
direction: direction/forward
top level: BBa_K1980008
start: 481
end: 486
orientation: inline

pCopA
type: SequenceAnnotation
persistent identity: annotation2528249
display id: annotation2528249
version: 1
top level: BBa_K1980008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1980008/annotation2528249/range2528249/1

https://synbiohub.org/public/igem/BBa_K1980008/annotation2528249/range2528249/1
type: Range
persistent identity: range2528249
display id: range2528249
version: 1
direction: direction/forward
top level: BBa_K1980008
start: 1
end: 51
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2528255
display id: annotation2528255
version: 1
top level: BBa_K1980008
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1980008/annotation2528255/range2528255/1

https://synbiohub.org/public/igem/BBa_K1980008/annotation2528255/range2528255/1
type: Range
persistent identity: range2528255
display id: range2528255
version: 1
direction: direction/forward
top level: BBa_K1980008
start: 1247
end: 1252
orientation: inline

Hexahistidine
type: SequenceAnnotation
persistent identity: annotation2528254
display id: annotation2528254
version: 1
top level: BBa_K1980008
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1980008/annotation2528254/range2528254/1

https://synbiohub.org/public/igem/BBa_K1980008/annotation2528254/range2528254/1
type: Range
persistent identity: range2528254
display id: range2528254
version: 1
direction: direction/forward
top level: BBa_K1980008
start: 1229
end: 1246
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2528252
display id: annotation2528252
version: 1
top level: BBa_K1980008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1980008/annotation2528252/range2528252/1

https://synbiohub.org/public/igem/BBa_K1980008/annotation2528252/range2528252/1
type: Range
persistent identity: range2528252
display id: range2528252
version: 1
direction: direction/forward
top level: BBa_K1980008
start: 515
end: 1228
orientation: inline

CueR
type: SequenceAnnotation
persistent identity: annotation2528251
display id: annotation2528251
version: 1
top level: BBa_K1980008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1980008/annotation2528251/range2528251/1

https://synbiohub.org/public/igem/BBa_K1980008/annotation2528251/range2528251/1
type: Range
persistent identity: range2528251
display id: range2528251
version: 1
direction: direction/forward
top level: BBa_K1980008
start: 76
end: 480
orientation: inline

BBa_K174016
type: ComponentDefinition
persistent identity: BBa K174016
display id: BBa_K174016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoterless ArsR binding site
creator: The Newcastle 2009 iGEM team
created: 2009-10-13T11:00:00Z
modified: 2015-07-24T01:17:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _277_
m user id: 4206
owner id: 3942
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part purely holds the binding site for ArsR protein which can be released when bound to different metals. Hence this part can be combined with any promoter to sense metals that can bind to ArsR protein. When added in front of a promoter with another metal sensor's protein's binding site, it can form an AND gate to sensitively detect specific metals.
mutable notes: It was developed mainly to use combinatorial approaches for metal sensing.
mutable provenance: ''B. subtilis''
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: ArsR binding site
sequence: https://synbiohub.org/public/igem/BBa_K174016_sequence/1

ArsR binding site
type: SequenceAnnotation
persistent identity: annotation2040774
display id: annotation2040774
version: 1
top level: BBa_K174016
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K174016/annotation2040774/range2040774/1

https://synbiohub.org/public/igem/BBa_K174016/annotation2040774/range2040774/1
type: Range
persistent identity: range2040774
display id: range2040774
version: 1
direction: direction/forward
top level: BBa_K174016
start: 9
end: 26
orientation: inline

pFe
type: ComponentDefinition
persistent identity: BBa I765000
display id: BBa_I765000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fe promoter
creator: Raul Cuero
created: 2007-10-25T11:00:00Z
modified: 2015-08-31T04:08:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _118_
m user id: 0
owner id: 2266
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Promoter regulated by iron presence
mutable notes: None
mutable provenance: Genomic sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: promoter
sequence: https://synbiohub.org/public/igem/BBa_I765000_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation1956037
display id: annotation1956037
version: 1
top level: pFe
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I765000/annotation1956037/range1956037/1

https://synbiohub.org/public/igem/BBa_I765000/annotation1956037/range1956037/1
type: Range
persistent identity: range1956037
display id: range1956037
version: 1
direction: direction/forward
top level: pFe
start: 1
end: 1045
orientation: inline

BBa_K1980004
type: ComponentDefinition
persistent identity: BBa K1980004
display id: BBa_K1980004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCusC promoter
creator: Sam Garforth
created: 2016-10-10T11:00:00Z
modified: 2016-10-23T07:20:32Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2447_
m user id: 29607
owner id: 29607
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is just the promoter region of our part pCusC mKate. This promoter is copper sensitive via the CusS/CusR two component system.
mutable notes: Much longer than a version in the registry previously.
mutable provenance: From E. coli genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: CusR binding
sequence annotation: -35
sequence annotation: pCusC
sequence: https://synbiohub.org/public/igem/BBa_K1980004_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation2525362
display id: annotation2525362
version: 1
top level: BBa_K1980004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1980004/annotation2525362/range2525362/1

https://synbiohub.org/public/igem/BBa_K1980004/annotation2525362/range2525362/1
type: Range
persistent identity: range2525362
display id: range2525362
version: 1
direction: direction/forward
top level: BBa_K1980004
start: 81
end: 87
orientation: inline

CusR binding
type: SequenceAnnotation
persistent identity: annotation2528663
display id: annotation2528663
version: 1
top level: BBa_K1980004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1980004/annotation2528663/range2528663/1

https://synbiohub.org/public/igem/BBa_K1980004/annotation2528663/range2528663/1
type: Range
persistent identity: range2528663
display id: range2528663
version: 1
direction: direction/forward
top level: BBa_K1980004
start: 30
end: 51
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2525363
display id: annotation2525363
version: 1
top level: BBa_K1980004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1980004/annotation2525363/range2525363/1

https://synbiohub.org/public/igem/BBa_K1980004/annotation2525363/range2525363/1
type: Range
persistent identity: range2525363
display id: range2525363
version: 1
direction: direction/forward
top level: BBa_K1980004
start: 60
end: 65
orientation: inline

pCusC
type: SequenceAnnotation
persistent identity: annotation2525361
display id: annotation2525361
version: 1
top level: BBa_K1980004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1980004/annotation2525361/range2525361/1

https://synbiohub.org/public/igem/BBa_K1980004/annotation2525361/range2525361/1
type: Range
persistent identity: range2525361
display id: range2525361
version: 1
direction: direction/forward
top level: BBa_K1980004
start: 1
end: 98
orientation: inline

BBa_K1460009
type: ComponentDefinition
persistent identity: BBa K1460009
display id: BBa_K1460009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Prcn + amilCP
creator: Eric Holmes
created: 2014-10-08T11:00:00Z
modified: 2015-07-14T12:34:05Z
discontinued: false
dominant: false
group u list: _1839_
m user id: 4206
owner id: 15649
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is the chromoprotein amilCP from Acropora millepora under control of the cobalt and nickel sensitive promoter Prcn.
mutable notes: This part is designed to quantify and detect high concentrations of cobalt or nickel in water.
mutable provenance: This is a composite part of the biobricks BBa_K540001 and BBa_K592009 from the 2011 Lyon Biosciences and 2011 Uppsala iGEM teams respectfully.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K592009
component: BBa_K540001
sequence annotation: BBa_K592009
sequence annotation: BBa_K540001
sequence: https://synbiohub.org/public/igem/BBa_K1460009_sequence/1

BBa_K592009
type: Component
persistent identity: component2408128
display id: component2408128
version: 1
top level: BBa_K1460009
access: public
definition: https://synbiohub.org/public/igem/BBa_K592009/1

BBa_K540001
type: Component
persistent identity: component2408126
display id: component2408126
version: 1
top level: BBa_K1460009
access: public
definition: BBa_K540001

BBa_K592009
type: SequenceAnnotation
persistent identity: annotation2408128
display id: annotation2408128
version: 1
top level: BBa_K1460009
location: https://synbiohub.org/public/igem/BBa_K1460009/annotation2408128/range2408128/1
component: BBa_K592009

https://synbiohub.org/public/igem/BBa_K1460009/annotation2408128/range2408128/1
type: Range
persistent identity: range2408128
display id: range2408128
version: 1
direction: direction/reverse
top level: BBa_K1460009
start: 433
end: 1101
orientation: inline

BBa_K540001
type: SequenceAnnotation
persistent identity: annotation2408126
display id: annotation2408126
version: 1
top level: BBa_K1460009
location: https://synbiohub.org/public/igem/BBa_K1460009/annotation2408126/range2408126/1
component: BBa_K540001

https://synbiohub.org/public/igem/BBa_K1460009/annotation2408126/range2408126/1
type: Range
persistent identity: range2408126
display id: range2408126
version: 1
direction: direction/reverse
top level: BBa_K1460009
start: 1
end: 426
orientation: inline

BBa_K1509001
type: ComponentDefinition
persistent identity: BBa K1509001
display id: BBa_K1509001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: A bi-directional promoter affected by SmtB protein
creator: Tong Li
created: 2014-09-26T11:00:00Z
modified: 2015-07-23T03:26:53Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1889_
m user id: 4206
owner id: 24032
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: smt O-P is a promoter which has two inverted repeats, designated S1/S2 and S3/S4, in the overlapping promoter/operator sites between gene smtA and gene smtB. The full promoter/operator DNA binds two SmtB dimmers forming a S1/S2-SmtB:SmtB-S3/S4 bridge complex. After binding with smtB, smt O-P represses the expression of downstream genes.
mutable notes: We got smt O-P and smtA, a fragment coding MT-like to sequester metal ions from smt locus by PCR and overlaped two fragments,smtB-smt O-P and pigment gene.Binding with smtB, smt O-P represses the expression of reporter genes.In the presence of heavy metal,the pigment and SmtA can expression normally.
mutable provenance: smt O-P is a part of smt locus from Synechococcus PCC7942.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: smt O-P
sequence: https://synbiohub.org/public/igem/BBa_K1509001_sequence/1

smt O-P
type: SequenceAnnotation
persistent identity: annotation2387085
display id: annotation2387085
version: 1
top level: BBa_K1509001
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1509001/annotation2387085/range2387085/1

https://synbiohub.org/public/igem/BBa_K1509001/annotation2387085/range2387085/1
type: Range
persistent identity: range2387085
display id: range2387085
version: 1
direction: direction/forward
top level: BBa_K1509001
start: 1
end: 100
orientation: inline

BBa_K1163107
type: ComponentDefinition
persistent identity: BBa K1163107
display id: BBa_K1163107
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fes promoter region
creator: Emiel van der Kouwe
created: 2013-09-21T11:00:00Z
modified: 2015-08-03T03:09:26Z
discontinued: false
dominant: false
group u list: _1475_
m user id: 4206
owner id: 14862
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoter from E. coli that controls the Fes gene, involved in iron uptake and iron sensitive. FUR (Ferric Uptake Regulation) is a transcriptional repressor of genes involved in iron homeostasis. In presence of iron, the FUR protein changes its conformation and dimerizes. This leads to the binding to the DNA on a FUR binding site, thus inhibiting the mRNA transcription. Why would you use this part? This promoter allows you to have an iron-sensitive behaviour. In fact, in the presence of iron, the Fes promoter region decreases the expression of the downstream gene.
mutable notes: We blindly extracted the 298 bp sequence upstream of the Fes gene in E. coli. It contains a putative FUR binding site and a putative RBS.
mutable provenance: Genomic DNA.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Fes
sequence: https://synbiohub.org/public/igem/BBa_K1163107_sequence/1

Fes
type: SequenceAnnotation
persistent identity: annotation2358151
display id: annotation2358151
version: 1
top level: BBa_K1163107
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1163107/annotation2358151/range2358151/1

https://synbiohub.org/public/igem/BBa_K1163107/annotation2358151/range2358151/1
type: Range
persistent identity: range2358151
display id: range2358151
version: 1
direction: direction/forward
top level: BBa_K1163107
start: 1
end: 298
orientation: inline

BBa_K1758354
type: ComponentDefinition
persistent identity: BBa K1758354
display id: BBa_K1758354
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Nickel responsive promoter with T7-promoter and UTR-sfGFP
creator: Team Bielefeld-CeBiTec 2015
created: 2015-09-07T11:00:00Z
modified: 2016-01-06T03:38:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2180_
m user id: 4206
owner id: 26630
owning group id: 9
status: status/Planning
bookmark: false
mutable description: ...
mutable notes: ...
mutable provenance: ...
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I719005
component: BBa_K1758352
sequence annotation: BBa_I719005
sequence annotation: BBa_K1758352
sequence: https://synbiohub.org/public/igem/BBa_K1758354_sequence/1

BBa_I719005
type: Component
persistent identity: component2447516
display id: component2447516
version: 1
top level: BBa_K1758354
access: public
definition: https://synbiohub.org/public/igem/BBa_I719005/1

BBa_K1758352
type: Component
persistent identity: component2447522
display id: component2447522
version: 1
top level: BBa_K1758354
access: public
definition: BBa_K1758352

BBa_I719005
type: SequenceAnnotation
persistent identity: annotation2447516
display id: annotation2447516
version: 1
top level: BBa_K1758354
location: https://synbiohub.org/public/igem/BBa_K1758354/annotation2447516/range2447516/1
component: BBa_I719005

https://synbiohub.org/public/igem/BBa_K1758354/annotation2447516/range2447516/1
type: Range
persistent identity: range2447516
display id: range2447516
version: 1
direction: direction/reverse
top level: BBa_K1758354
start: 1
end: 23
orientation: inline

BBa_K1758352
type: SequenceAnnotation
persistent identity: annotation2447522
display id: annotation2447522
version: 1
top level: BBa_K1758354
location: https://synbiohub.org/public/igem/BBa_K1758354/annotation2447522/range2447522/1
component: BBa_K1758352

https://synbiohub.org/public/igem/BBa_K1758354/annotation2447522/range2447522/1
type: Range
persistent identity: range2447522
display id: range2447522
version: 1
direction: direction/reverse
top level: BBa_K1758354
start: 30
end: 1042
orientation: inline

BBa_K1758314
type: ComponentDefinition
persistent identity: BBa K1758314
display id: BBa_K1758314
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Chromium responsive promoter under T7-promoter with UTR-sfGFP
creator: Team Bielefeld-CeBiTec 2015
created: 2015-09-07T11:00:00Z
modified: 2016-01-06T03:34:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2180_
m user id: 4206
owner id: 26630
owning group id: 9
status: status/Planning
bookmark: false
mutable description: T7 controlled Chromium responsive promoter with 5??untranslated region and sfGFP for detection
mutable notes: ...
mutable provenance: ...
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I719005
component: BBa_K1758312
sequence annotation: BBa_I719005
sequence annotation: BBa_K1758312
sequence: https://synbiohub.org/public/igem/BBa_K1758314_sequence/1

BBa_I719005
type: Component
persistent identity: component2474963
display id: component2474963
version: 1
top level: BBa_K1758314
access: public
definition: https://synbiohub.org/public/igem/BBa_I719005/1

BBa_K1758312
type: Component
persistent identity: component2474969
display id: component2474969
version: 1
top level: BBa_K1758314
access: public
definition: BBa_K1758312

BBa_I719005
type: SequenceAnnotation
persistent identity: annotation2474963
display id: annotation2474963
version: 1
top level: BBa_K1758314
location: https://synbiohub.org/public/igem/BBa_K1758314/annotation2474963/range2474963/1
component: BBa_I719005

https://synbiohub.org/public/igem/BBa_K1758314/annotation2474963/range2474963/1
type: Range
persistent identity: range2474963
display id: range2474963
version: 1
direction: direction/reverse
top level: BBa_K1758314
start: 1
end: 23
orientation: inline

BBa_K1758312
type: SequenceAnnotation
persistent identity: annotation2474969
display id: annotation2474969
version: 1
top level: BBa_K1758314
location: https://synbiohub.org/public/igem/BBa_K1758314/annotation2474969/range2474969/1
component: BBa_K1758312

https://synbiohub.org/public/igem/BBa_K1758314/annotation2474969/range2474969/1
type: Range
persistent identity: range2474969
display id: range2474969
version: 1
direction: direction/reverse
top level: BBa_K1758314
start: 32
end: 1104
orientation: inline

BBa_K346054
type: ComponentDefinition
persistent identity: BBa K346054
display id: BBa_K346054
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PpbrA promoter
creator: Qianzhu Wu
created: 2010-10-17T11:00:00Z
modified: 2015-08-03T03:07:22Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _457_
m user id: 4206
owner id: 5916
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: PpbrA promoter
mutable notes: PpbrA promoter
mutable provenance: PpbrA promoter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K346054_sequence/1

Fur box
type: ComponentDefinition
persistent identity: BBa K1122069
display id: BBa_K1122069
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ferric uptake repressor box
creator: Hugo Villanueva
created: 2013-07-23T11:00:00Z
modified: 2015-07-24T01:15:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1433_
m user id: 4206
owner id: 18022
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Ferric uptake repressor box (Fur box). When the ferric uptake repressor binds to it, it represses transcription.
mutable notes: Fur boxes differ, we used the consensus sequence which per se is not an actual sequence occuring within B. subtilis but rather the strongest sequence that Fur protein would bind to.
mutable provenance: Bacillus subtilis Fur box consensus sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1122069_sequence/1

BBa_K1163101
type: ComponentDefinition
persistent identity: BBa K1163101
display id: BBa_K1163101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pAceB promoter region
creator: Emiel van der Kouwe
created: 2013-09-21T11:00:00Z
modified: 2015-08-03T03:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1475_
m user id: 4206
owner id: 14862
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: Promoter from E. coli that controls the AceB gene, involved in iron uptake and iron sensitive. FUR (Ferric Uptake Regulation) is a transcriptional repressor of genes involved in iron homeostasis. In presence of iron, the FUR protein changes its conformation and dimerizes. This leads to the binding to the DNA on a FUR binding site, thus inhibiting the mRNA transcription. Why would you use this part? This promoter allows you to have an iron-sensitive behaviour. In fact, in the presence of iron, the Fes promoter region decreases the expression of the downstream gene.
mutable notes: We blindly extracted the 300 bp sequence upstream of the AceB gene in E. coli. It contains a putative FUR binding site and a putative RBS.
mutable provenance: Genomic DNA.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: pAceB
sequence: https://synbiohub.org/public/igem/BBa_K1163101_sequence/1

pAceB
type: SequenceAnnotation
persistent identity: annotation2358132
display id: annotation2358132
version: 1
top level: BBa_K1163101
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1163101/annotation2358132/range2358132/1

https://synbiohub.org/public/igem/BBa_K1163101/annotation2358132/range2358132/1
type: Range
persistent identity: range2358132
display id: range2358132
version: 1
direction: direction/forward
top level: BBa_K1163101
start: 1
end: 300
orientation: inline

BBa_K1758333
type: ComponentDefinition
persistent identity: BBa K1758333
display id: BBa_K1758333
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lead repressor under control of constitutive promoter and strong RBS and lead responsive promoter wi
creator: Team Bielefeld-CeBiTec 2015
created: 2015-08-29T11:00:00Z
modified: 2016-01-06T03:41:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2180_
m user id: 4206
owner id: 26630
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Repressor for lead responsive promoter under the control of konstitutive promoter and lead responsive promoter with 5??untranslated region and sfGFP for detection. As a lead biosensor
mutable notes: ??...
mutable provenance: genomic sequence out of ????Cupriavidus metallidurans???? CH34
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1758332
component: BBa_K1758330
sequence annotation: BBa_K1758332
sequence annotation: BBa_K1758330
sequence: https://synbiohub.org/public/igem/BBa_K1758333_sequence/1

BBa_K1758332
type: Component
persistent identity: component2440128
display id: component2440128
version: 1
top level: BBa_K1758333
access: public
definition: BBa_K1758332

BBa_K1758330
type: Component
persistent identity: component2440122
display id: component2440122
version: 1
top level: BBa_K1758333
access: public
definition: https://synbiohub.org/public/igem/BBa_K1758330/1

BBa_K1758332
type: SequenceAnnotation
persistent identity: annotation2440128
display id: annotation2440128
version: 1
top level: BBa_K1758333
location: https://synbiohub.org/public/igem/BBa_K1758333/annotation2440128/range2440128/1
component: BBa_K1758332

https://synbiohub.org/public/igem/BBa_K1758333/annotation2440128/range2440128/1
type: Range
persistent identity: range2440128
display id: range2440128
version: 1
direction: direction/reverse
top level: BBa_K1758333
start: 508
end: 1484
orientation: inline

BBa_K1758330
type: SequenceAnnotation
persistent identity: annotation2440122
display id: annotation2440122
version: 1
top level: BBa_K1758333
location: https://synbiohub.org/public/igem/BBa_K1758333/annotation2440122/range2440122/1
component: BBa_K1758330

https://synbiohub.org/public/igem/BBa_K1758333/annotation2440122/range2440122/1
type: Range
persistent identity: range2440122
display id: range2440122
version: 1
direction: direction/reverse
top level: BBa_K1758333
start: 1
end: 499
orientation: inline

BBa_K1758335
type: ComponentDefinition
persistent identity: BBa K1758335
display id: BBa_K1758335
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: UTR-sfGFP controlled by T7-promoter and lead responsive promoter
creator: Team Bielefeld-CeBiTec 2015
created: 2015-09-08T11:00:00Z
modified: 2016-01-06T03:41:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2180_
m user id: 4206
owner id: 26630
owning group id: 9
status: status/Planning
bookmark: false
mutable description: lead induceble promoter under the control of T7 Promoter with mRFP for detection
mutable notes: ...
mutable provenance: This part consists of T7 promoter lead induceble Promoter PbrA from ''Cupriavidus metallidurans CH34'' and mRFP for detection
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I719005
component: BBa_K1758331
sequence annotation: BBa_I719005
sequence annotation: BBa_K1758331
sequence: https://synbiohub.org/public/igem/BBa_K1758335_sequence/1

BBa_I719005
type: Component
persistent identity: component2447787
display id: component2447787
version: 1
top level: BBa_K1758335
access: public
definition: https://synbiohub.org/public/igem/BBa_I719005/1

BBa_K1758331
type: Component
persistent identity: component2447790
display id: component2447790
version: 1
top level: BBa_K1758335
access: public
definition: BBa_K1758331

BBa_I719005
type: SequenceAnnotation
persistent identity: annotation2447787
display id: annotation2447787
version: 1
top level: BBa_K1758335
location: https://synbiohub.org/public/igem/BBa_K1758335/annotation2447787/range2447787/1
component: BBa_I719005

https://synbiohub.org/public/igem/BBa_K1758335/annotation2447787/range2447787/1
type: Range
persistent identity: range2447787
display id: range2447787
version: 1
direction: direction/reverse
top level: BBa_K1758335
start: 1
end: 23
orientation: inline

BBa_K1758331
type: SequenceAnnotation
persistent identity: annotation2447790
display id: annotation2447790
version: 1
top level: BBa_K1758335
location: https://synbiohub.org/public/igem/BBa_K1758335/annotation2447790/range2447790/1
component: BBa_K1758331

https://synbiohub.org/public/igem/BBa_K1758335/annotation2447790/range2447790/1
type: Range
persistent identity: range2447790
display id: range2447790
version: 1
direction: direction/reverse
top level: BBa_K1758335
start: 32
end: 786
orientation: inline

BBa_K1758313
type: ComponentDefinition
persistent identity: BBa K1758313
display id: BBa_K1758313
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Chromium repressor under control of constitutive promoter and strong RBS,chromium responsive promote
creator: Team Bielefeld-CeBiTec 2015
created: 2015-08-29T11:00:00Z
modified: 2016-01-06T03:36:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2180_
m user id: 4206
owner id: 26630
owning group id: 9
status: status/Planning
bookmark: true
mutable description: a divice consisting of chromium repressor under the control of konstitutive promoter, the chromium responsive promoter a 5??untranslated region and sfGFP for detectin
mutable notes: ...
mutable provenance: this device is inspired by K10580008 a chromium responsive system out of ????Ochrobactrum tritici 5bvl1????. We cominedd the chromium VI responsive promoter with a ????E.coli???? codon optimised repressor sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1758312
component: BBa_K1758310
sequence annotation: BBa_K1758310
sequence annotation: BBa_K1758312
sequence: https://synbiohub.org/public/igem/BBa_K1758313_sequence/1

BBa_K1758312
type: Component
persistent identity: component2440100
display id: component2440100
version: 1
top level: BBa_K1758313
access: public
definition: BBa_K1758312

BBa_K1758310
type: Component
persistent identity: component2440094
display id: component2440094
version: 1
top level: BBa_K1758313
access: public
definition: https://synbiohub.org/public/igem/BBa_K1758310/1

BBa_K1758310
type: SequenceAnnotation
persistent identity: annotation2440094
display id: annotation2440094
version: 1
top level: BBa_K1758313
location: https://synbiohub.org/public/igem/BBa_K1758313/annotation2440094/range2440094/1
component: BBa_K1758310

https://synbiohub.org/public/igem/BBa_K1758313/annotation2440094/range2440094/1
type: Range
persistent identity: range2440094
display id: range2440094
version: 1
direction: direction/reverse
top level: BBa_K1758313
start: 1
end: 1000
orientation: inline

BBa_K1758312
type: SequenceAnnotation
persistent identity: annotation2440100
display id: annotation2440100
version: 1
top level: BBa_K1758313
location: https://synbiohub.org/public/igem/BBa_K1758313/annotation2440100/range2440100/1
component: BBa_K1758312

https://synbiohub.org/public/igem/BBa_K1758313/annotation2440100/range2440100/1
type: Range
persistent identity: range2440100
display id: range2440100
version: 1
direction: direction/reverse
top level: BBa_K1758313
start: 1009
end: 2081
orientation: inline

BBa_K1758331
type: ComponentDefinition
persistent identity: BBa K1758331
display id: BBa_K1758331
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lead responsive promoter with mRFP
creator: Team Bielefeld-CeBiTec 2015
created: 2015-08-29T11:00:00Z
modified: 2016-01-06T03:42:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2180_
m user id: 4206
owner id: 26630
owning group id: 9
status: status/Planning
bookmark: false
mutable description: lead responsive promoter with mRFP for detection
mutable notes: ...
mutable provenance: lead responsive promoter of ????Cupriavidus metallidurans???? CH34
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: mRFP
sequence annotation: pbrA
sequence: https://synbiohub.org/public/igem/BBa_K1758331_sequence/1

mRFP
type: SequenceAnnotation
persistent identity: annotation2440073
display id: annotation2440073
version: 1
top level: BBa_K1758331
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1758331/annotation2440073/range2440073/1

https://synbiohub.org/public/igem/BBa_K1758331/annotation2440073/range2440073/1
type: Range
persistent identity: range2440073
display id: range2440073
version: 1
direction: direction/forward
top level: BBa_K1758331
start: 75
end: 755
orientation: inline

pbrA
type: SequenceAnnotation
persistent identity: annotation2440072
display id: annotation2440072
version: 1
top level: BBa_K1758331
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1758331/annotation2440072/range2440072/1

https://synbiohub.org/public/igem/BBa_K1758331/annotation2440072/range2440072/1
type: Range
persistent identity: range2440072
display id: range2440072
version: 1
direction: direction/forward
top level: BBa_K1758331
start: 1
end: 68
orientation: inline

BBa_K1758321
type: ComponentDefinition
persistent identity: BBa K1758321
display id: BBa_K1758321
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mRFP under control of copper responsive promoter
creator: Team Bielefeld-CeBiTec 2015
created: 2015-08-28T11:00:00Z
modified: 2016-01-06T03:42:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2180_
m user id: 4206
owner id: 26630
owning group id: 9
status: status/Planning
bookmark: false
mutable description: A copper iduceble promoter with RFP for detection
mutable notes: ---
mutable provenance: CopAp is a copper induceble promoter out of ``E.coli`` K12
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: mRFP
sequence annotation: CopAP
sequence: https://synbiohub.org/public/igem/BBa_K1758321_sequence/1

mRFP
type: SequenceAnnotation
persistent identity: annotation2440067
display id: annotation2440067
version: 1
top level: BBa_K1758321
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1758321/annotation2440067/range2440067/1

https://synbiohub.org/public/igem/BBa_K1758321/annotation2440067/range2440067/1
type: Range
persistent identity: range2440067
display id: range2440067
version: 1
direction: direction/forward
top level: BBa_K1758321
start: 90
end: 770
orientation: inline

CopAP
type: SequenceAnnotation
persistent identity: annotation2440066
display id: annotation2440066
version: 1
top level: BBa_K1758321
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1758321/annotation2440066/range2440066/1

https://synbiohub.org/public/igem/BBa_K1758321/annotation2440066/range2440066/1
type: Range
persistent identity: range2440066
display id: range2440066
version: 1
direction: direction/forward
top level: BBa_K1758321
start: 1
end: 83
orientation: inline

BBa_K346002
type: ComponentDefinition
persistent identity: BBa K346002
display id: BBa_K346002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PmerT promoter (mercury-responsive)
creator: Qianzhu Wu & Mei Chen
created: 2010-10-11T11:00:00Z
modified: 2015-07-14T12:33:51Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _457_
m user id: 4206
owner id: 4411
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part was isolated from Tn21. When regulated by its cognate transcription factor MerR, it will represent a dose-response manner in response to the concentration of mercury.
mutable notes: It was divergently regulated by MerR
mutable provenance: TTCCATATCGCTTGACTCCGTACATGAGTACGGAAGTAAGGTTACGCTATCCAATCC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: promoter
sequence: https://synbiohub.org/public/igem/BBa_K346002_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation2085551
display id: annotation2085551
version: 1
top level: BBa_K346002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K346002/annotation2085551/range2085551/1

https://synbiohub.org/public/igem/BBa_K346002/annotation2085551/range2085551/1
type: Range
persistent identity: range2085551
display id: range2085551
version: 1
direction: direction/forward
top level: BBa_K346002
start: 1
end: 57
orientation: inline

BBa_K1758351
type: ComponentDefinition
persistent identity: BBa K1758351
display id: BBa_K1758351
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Nickel responsive promoter with RFP
creator: Team Bielefeld-CeBiTec 2015
created: 2015-08-29T11:00:00Z
modified: 2016-01-06T03:38:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2180_
m user id: 4206
owner id: 26630
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Nickel responsive Promoter with mRFP for detection
mutable notes: ...
mutable provenance: design based on K549001
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: Rcna
sequence annotation: mRFP
sequence: https://synbiohub.org/public/igem/BBa_K1758351_sequence/1

Rcna
type: SequenceAnnotation
persistent identity: annotation2440070
display id: annotation2440070
version: 1
top level: BBa_K1758351
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1758351/annotation2440070/range2440070/1

https://synbiohub.org/public/igem/BBa_K1758351/annotation2440070/range2440070/1
type: Range
persistent identity: range2440070
display id: range2440070
version: 1
direction: direction/forward
top level: BBa_K1758351
start: 1
end: 104
orientation: inline

mRFP
type: SequenceAnnotation
persistent identity: annotation2440071
display id: annotation2440071
version: 1
top level: BBa_K1758351
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1758351/annotation2440071/range2440071/1

https://synbiohub.org/public/igem/BBa_K1758351/annotation2440071/range2440071/1
type: Range
persistent identity: range2440071
display id: range2440071
version: 1
direction: direction/forward
top level: BBa_K1758351
start: 111
end: 791
orientation: inline

BBa_K174017
type: ComponentDefinition
persistent identity: BBa K174017
display id: BBa_K174017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CadA promoter with CzrA binding site
creator: The Newcastle 2009 iGEM team
created: 2009-10-13T11:00:00Z
modified: 2015-07-24T01:17:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _277_
m user id: 4206
owner id: 3942
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: In ''B. subtilis'' CadA system works as an efflux system to take the metals out in the cell. It is normally repressed by CzrA repressor protein. When certain metals get inside the cell, they can bind to CzrA and derepress the promoter expressing CadA. Hence metals are taken out by the efflux system. By taking the promoter of CadA system, we created a generic metal sensor. Hence when it is combined with coding sequences of other genes, it will regulate the expression of these genes upon sensing metals.
mutable notes: It was developed mainly to use combinatorial approaches for metal sensing
mutable provenance: It is taken from ''B. subtilis'' ''cadA'' promoter.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: sigA -10
sequence annotation: cadA promoter
sequence annotation: sigA -35
sequence annotation: CzrA binding site
sequence: https://synbiohub.org/public/igem/BBa_K174017_sequence/1

sigA -10
type: SequenceAnnotation
persistent identity: annotation2040829
display id: annotation2040829
version: 1
top level: BBa_K174017
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K174017/annotation2040829/range2040829/1

https://synbiohub.org/public/igem/BBa_K174017/annotation2040829/range2040829/1
type: Range
persistent identity: range2040829
display id: range2040829
version: 1
direction: direction/forward
top level: BBa_K174017
start: 42
end: 47
orientation: inline

cadA promoter
type: SequenceAnnotation
persistent identity: annotation2040827
display id: annotation2040827
version: 1
top level: BBa_K174017
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K174017/annotation2040827/range2040827/1

https://synbiohub.org/public/igem/BBa_K174017/annotation2040827/range2040827/1
type: Range
persistent identity: range2040827
display id: range2040827
version: 1
direction: direction/forward
top level: BBa_K174017
start: 11
end: 144
orientation: inline

sigA -35
type: SequenceAnnotation
persistent identity: annotation2040828
display id: annotation2040828
version: 1
top level: BBa_K174017
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K174017/annotation2040828/range2040828/1

https://synbiohub.org/public/igem/BBa_K174017/annotation2040828/range2040828/1
type: Range
persistent identity: range2040828
display id: range2040828
version: 1
direction: direction/forward
top level: BBa_K174017
start: 18
end: 23
orientation: inline

CzrA binding site
type: SequenceAnnotation
persistent identity: annotation2040830
display id: annotation2040830
version: 1
top level: BBa_K174017
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K174017/annotation2040830/range2040830/1

https://synbiohub.org/public/igem/BBa_K174017/annotation2040830/range2040830/1
type: Range
persistent identity: range2040830
display id: range2040830
version: 1
direction: direction/forward
top level: BBa_K174017
start: 56
end: 86
orientation: inline

BBa_K1758353
type: ComponentDefinition
persistent identity: BBa K1758353
display id: BBa_K1758353
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Nickel repressor under control of constitutive promoter and strong RBS and Nickel responsive promote
creator: Team Bielefeld-CeBiTec 2015
created: 2015-09-07T11:00:00Z
modified: 2016-01-06T03:38:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2180_
m user id: 4206
owner id: 26630
owning group id: 9
status: status/Planning
bookmark: false
mutable description: ...
mutable notes: ...
mutable provenance: ...
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1758350
component: BBa_K1758352
sequence annotation: BBa_K1758352
sequence annotation: BBa_K1758350
sequence: https://synbiohub.org/public/igem/BBa_K1758353_sequence/1

BBa_K1758350
type: Component
persistent identity: component2447509
display id: component2447509
version: 1
top level: BBa_K1758353
access: public
definition: https://synbiohub.org/public/igem/BBa_K1758350/1

BBa_K1758352
type: Component
persistent identity: component2447515
display id: component2447515
version: 1
top level: BBa_K1758353
access: public
definition: BBa_K1758352

BBa_K1758352
type: SequenceAnnotation
persistent identity: annotation2447515
display id: annotation2447515
version: 1
top level: BBa_K1758353
location: https://synbiohub.org/public/igem/BBa_K1758353/annotation2447515/range2447515/1
component: BBa_K1758352

https://synbiohub.org/public/igem/BBa_K1758353/annotation2447515/range2447515/1
type: Range
persistent identity: range2447515
display id: range2447515
version: 1
direction: direction/reverse
top level: BBa_K1758353
start: 341
end: 1353
orientation: inline

BBa_K1758350
type: SequenceAnnotation
persistent identity: annotation2447509
display id: annotation2447509
version: 1
top level: BBa_K1758353
location: https://synbiohub.org/public/igem/BBa_K1758353/annotation2447509/range2447509/1
component: BBa_K1758350

https://synbiohub.org/public/igem/BBa_K1758353/annotation2447509/range2447509/1
type: Range
persistent identity: range2447509
display id: range2447509
version: 1
direction: direction/reverse
top level: BBa_K1758353
start: 1
end: 334
orientation: inline

BBa_K1980005
type: ComponentDefinition
persistent identity: BBa K1980005
display id: BBa_K1980005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCopA sfGFP
creator: Sam Garforth
created: 2016-10-10T11:00:00Z
modified: 2016-10-23T04:46:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2447_
m user id: 29607
owner id: 29607
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is the copper sensitive promoter pCopA (controlled by CueR) in front of an RBS and a sfGFP reporter
mutable notes: Codon optised to E. coli.
mutable provenance: E. coli genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Stop
sequence annotation: CueR binding
sequence annotation: pCopA
sequence annotation: RBS
sequence annotation: Hexahis
sequence annotation: sfGFP
sequence: https://synbiohub.org/public/igem/BBa_K1980005_sequence/1

Stop
type: SequenceAnnotation
persistent identity: annotation2528243
display id: annotation2528243
version: 1
top level: BBa_K1980005
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1980005/annotation2528243/range2528243/1

https://synbiohub.org/public/igem/BBa_K1980005/annotation2528243/range2528243/1
type: Range
persistent identity: range2528243
display id: range2528243
version: 1
direction: direction/forward
top level: BBa_K1980005
start: 805
end: 810
orientation: inline

CueR binding
type: SequenceAnnotation
persistent identity: annotation2528664
display id: annotation2528664
version: 1
top level: BBa_K1980005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1980005/annotation2528664/range2528664/1

https://synbiohub.org/public/igem/BBa_K1980005/annotation2528664/range2528664/1
type: Range
persistent identity: range2528664
display id: range2528664
version: 1
direction: direction/forward
top level: BBa_K1980005
start: 19
end: 37
orientation: inline

pCopA
type: SequenceAnnotation
persistent identity: annotation2528239
display id: annotation2528239
version: 1
top level: BBa_K1980005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1980005/annotation2528239/range2528239/1

https://synbiohub.org/public/igem/BBa_K1980005/annotation2528239/range2528239/1
type: Range
persistent identity: range2528239
display id: range2528239
version: 1
direction: direction/forward
top level: BBa_K1980005
start: 1
end: 51
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2528240
display id: annotation2528240
version: 1
top level: BBa_K1980005
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1980005/annotation2528240/range2528240/1

https://synbiohub.org/public/igem/BBa_K1980005/annotation2528240/range2528240/1
type: Range
persistent identity: range2528240
display id: range2528240
version: 1
direction: direction/forward
top level: BBa_K1980005
start: 57
end: 62
orientation: inline

Hexahis
type: SequenceAnnotation
persistent identity: annotation2528242
display id: annotation2528242
version: 1
top level: BBa_K1980005
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1980005/annotation2528242/range2528242/1

https://synbiohub.org/public/igem/BBa_K1980005/annotation2528242/range2528242/1
type: Range
persistent identity: range2528242
display id: range2528242
version: 1
direction: direction/forward
top level: BBa_K1980005
start: 787
end: 804
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2528241
display id: annotation2528241
version: 1
top level: BBa_K1980005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1980005/annotation2528241/range2528241/1

https://synbiohub.org/public/igem/BBa_K1980005/annotation2528241/range2528241/1
type: Range
persistent identity: range2528241
display id: range2528241
version: 1
direction: direction/forward
top level: BBa_K1980005
start: 73
end: 786
orientation: inline

BBa_K1758322
type: ComponentDefinition
persistent identity: BBa K1758322
display id: BBa_K1758322
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Copper activator under control constitutive promoter and strong RBS and Copper responsive promoter w
creator: Team Bielefeld-CeBiTec 2015
created: 2015-08-29T11:00:00Z
modified: 2016-01-06T03:42:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2180_
m user id: 4206
owner id: 26630
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This device consists of the Aktivator CueR for the copper responsive promoter CopAP and mRFP for detection. It works as a copper sensor
mutable notes: ...
mutable provenance: activator and promoter are part of ????E.coli`` K12 copper efflux system
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1758320
component: BBa_K1758321
sequence annotation: BBa_K1758321
sequence annotation: BBa_K1758320
sequence: https://synbiohub.org/public/igem/BBa_K1758322_sequence/1

BBa_K1758320
type: Component
persistent identity: component2440116
display id: component2440116
version: 1
top level: BBa_K1758322
access: public
definition: https://synbiohub.org/public/igem/BBa_K1758320/1

BBa_K1758321
type: Component
persistent identity: component2440119
display id: component2440119
version: 1
top level: BBa_K1758322
access: public
definition: BBa_K1758321

BBa_K1758321
type: SequenceAnnotation
persistent identity: annotation2440119
display id: annotation2440119
version: 1
top level: BBa_K1758322
location: https://synbiohub.org/public/igem/BBa_K1758322/annotation2440119/range2440119/1
component: BBa_K1758321

https://synbiohub.org/public/igem/BBa_K1758322/annotation2440119/range2440119/1
type: Range
persistent identity: range2440119
display id: range2440119
version: 1
direction: direction/reverse
top level: BBa_K1758322
start: 478
end: 1247
orientation: inline

BBa_K1758320
type: SequenceAnnotation
persistent identity: annotation2440116
display id: annotation2440116
version: 1
top level: BBa_K1758322
location: https://synbiohub.org/public/igem/BBa_K1758322/annotation2440116/range2440116/1
component: BBa_K1758320

https://synbiohub.org/public/igem/BBa_K1758322/annotation2440116/range2440116/1
type: Range
persistent identity: range2440116
display id: range2440116
version: 1
direction: direction/reverse
top level: BBa_K1758322
start: 1
end: 469
orientation: inline

metalP
type: ComponentDefinition
persistent identity: BBa K1342005
display id: BBa_K1342005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: zinTp (Cd2+ sensing promoter) (Fw:Lac promoter(-) ver.)
creator: Daiki Haraguchi,Naoki Saito,Yoshiharu Otaki,Ryuhei Minei
created: 2014-10-02T11:00:00Z
modified: 2015-07-23T03:06:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1717_
m user id: 4206
owner id: 22434
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: This
mutable notes: soon
mutable provenance: it
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: promoter
sequence: https://synbiohub.org/public/igem/BBa_K1342005_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation2393954
display id: annotation2393954
version: 1
top level: metalP
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1342005/annotation2393954/range2393954/1

https://synbiohub.org/public/igem/BBa_K1342005/annotation2393954/range2393954/1
type: Range
persistent identity: range2393954
display id: range2393954
version: 1
direction: direction/forward
top level: metalP
start: 1
end: 81
orientation: inline

BBa_K1460010
type: ComponentDefinition
persistent identity: BBa K1460010
display id: BBa_K1460010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PmerT + amilCP
creator: Eric Holmes
created: 2014-10-08T11:00:00Z
modified: 2015-07-14T12:32:45Z
discontinued: false
dominant: false
group u list: _1839_
m user id: 4206
owner id: 15649
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is the chromoprotein amilCP from Acropora millepora under control of the mercury sensitive promoter PmerT.
mutable notes: This part is designed for quantification and visual detection of high mercury concentrations in water.
mutable provenance: This part is a composite of BBa_K346002 and BBa_K592009 from the 2010 Peking and 2011 Uppsala iGEM teams respectively.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K592009
component: BBa_K346002
sequence annotation: BBa_K346002
sequence annotation: BBa_K592009
sequence: https://synbiohub.org/public/igem/BBa_K1460010_sequence/1

BBa_K592009
type: Component
persistent identity: component2408133
display id: component2408133
version: 1
top level: BBa_K1460010
access: public
definition: https://synbiohub.org/public/igem/BBa_K592009/1

BBa_K346002
type: Component
persistent identity: component2408131
display id: component2408131
version: 1
top level: BBa_K1460010
access: public
definition: BBa_K346002

BBa_K346002
type: SequenceAnnotation
persistent identity: annotation2408131
display id: annotation2408131
version: 1
top level: BBa_K1460010
location: https://synbiohub.org/public/igem/BBa_K1460010/annotation2408131/range2408131/1
component: BBa_K346002

https://synbiohub.org/public/igem/BBa_K1460010/annotation2408131/range2408131/1
type: Range
persistent identity: range2408131
display id: range2408131
version: 1
direction: direction/reverse
top level: BBa_K1460010
start: 1
end: 57
orientation: inline

BBa_K592009
type: SequenceAnnotation
persistent identity: annotation2408133
display id: annotation2408133
version: 1
top level: BBa_K1460010
location: https://synbiohub.org/public/igem/BBa_K1460010/annotation2408133/range2408133/1
component: BBa_K592009

https://synbiohub.org/public/igem/BBa_K1460010/annotation2408133/range2408133/1
type: Range
persistent identity: range2408133
display id: range2408133
version: 1
direction: direction/reverse
top level: BBa_K1460010
start: 64
end: 732
orientation: inline

BBa_K1758343
type: ComponentDefinition
persistent identity: BBa K1758343
display id: BBa_K1758343
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MerR activator under constitutive promoter and induceble merT promoter with 5 UTR -sfGFP
creator: Team Bielefeld-CeBiTec 2015
created: 2015-09-07T11:00:00Z
modified: 2016-01-06T03:41:10Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2180_
m user id: 4206
owner id: 26630
owning group id: 9
status: status/Planning
bookmark: false
mutable description: ...
mutable notes: ...
mutable provenance: ...
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1758340
component: BBa_K1758342
sequence annotation: BBa_K1758340
sequence annotation: BBa_K1758342
sequence: https://synbiohub.org/public/igem/BBa_K1758343_sequence/1

BBa_K1758340
type: Component
persistent identity: component2447498
display id: component2447498
version: 1
top level: BBa_K1758343
access: public
definition: https://synbiohub.org/public/igem/BBa_K1758340/1

BBa_K1758342
type: Component
persistent identity: component2447504
display id: component2447504
version: 1
top level: BBa_K1758343
access: public
definition: BBa_K1758342

BBa_K1758340
type: SequenceAnnotation
persistent identity: annotation2447498
display id: annotation2447498
version: 1
top level: BBa_K1758343
location: https://synbiohub.org/public/igem/BBa_K1758343/annotation2447498/range2447498/1
component: BBa_K1758340

https://synbiohub.org/public/igem/BBa_K1758343/annotation2447498/range2447498/1
type: Range
persistent identity: range2447498
display id: range2447498
version: 1
direction: direction/reverse
top level: BBa_K1758343
start: 1
end: 496
orientation: inline

BBa_K1758342
type: SequenceAnnotation
persistent identity: annotation2447504
display id: annotation2447504
version: 1
top level: BBa_K1758343
location: https://synbiohub.org/public/igem/BBa_K1758343/annotation2447504/range2447504/1
component: BBa_K1758342

https://synbiohub.org/public/igem/BBa_K1758343/annotation2447504/range2447504/1
type: Range
persistent identity: range2447504
display id: range2447504
version: 1
direction: direction/reverse
top level: BBa_K1758343
start: 505
end: 1470
orientation: inline

BBa_K1758332
type: ComponentDefinition
persistent identity: BBa K1758332
display id: BBa_K1758332
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lead responsive promoter with UTR-sfGFP
creator: Team Bielefeld-CeBiTec 2015
created: 2015-08-28T11:00:00Z
modified: 2016-01-06T03:41:52Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2180_
m user id: 4206
owner id: 26630
owning group id: 9
status: status/Planning
bookmark: false
mutable description: lead responsive promoter with 5??untranslated region and sfGFP for detection
mutable notes: ...
mutable provenance: genomic sequence of ``cupriavidus metallidurans CH34``
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: T7-term
sequence annotation: T1
sequence annotation: 5??UTR
sequence annotation: sfGFP
sequence annotation: PbrA
sequence: https://synbiohub.org/public/igem/BBa_K1758332_sequence/1

T7-term
type: SequenceAnnotation
persistent identity: annotation2439975
display id: annotation2439975
version: 1
top level: BBa_K1758332
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1758332/annotation2439975/range2439975/1

https://synbiohub.org/public/igem/BBa_K1758332/annotation2439975/range2439975/1
type: Range
persistent identity: range2439975
display id: range2439975
version: 1
direction: direction/forward
top level: BBa_K1758332
start: 937
end: 977
orientation: inline

T1
type: SequenceAnnotation
persistent identity: annotation2439974
display id: annotation2439974
version: 1
top level: BBa_K1758332
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1758332/annotation2439974/range2439974/1

https://synbiohub.org/public/igem/BBa_K1758332/annotation2439974/range2439974/1
type: Range
persistent identity: range2439974
display id: range2439974
version: 1
direction: direction/forward
top level: BBa_K1758332
start: 849
end: 928
orientation: inline

5??UTR
type: SequenceAnnotation
persistent identity: annotation2439972
display id: annotation2439972
version: 1
top level: BBa_K1758332
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1758332/annotation2439972/range2439972/1

https://synbiohub.org/public/igem/BBa_K1758332/annotation2439972/range2439972/1
type: Range
persistent identity: range2439972
display id: range2439972
version: 1
direction: direction/forward
top level: BBa_K1758332
start: 75
end: 120
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2439973
display id: annotation2439973
version: 1
top level: BBa_K1758332
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1758332/annotation2439973/range2439973/1

https://synbiohub.org/public/igem/BBa_K1758332/annotation2439973/range2439973/1
type: Range
persistent identity: range2439973
display id: range2439973
version: 1
direction: direction/forward
top level: BBa_K1758332
start: 121
end: 840
orientation: inline

PbrA
type: SequenceAnnotation
persistent identity: annotation2439971
display id: annotation2439971
version: 1
top level: BBa_K1758332
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1758332/annotation2439971/range2439971/1

https://synbiohub.org/public/igem/BBa_K1758332/annotation2439971/range2439971/1
type: Range
persistent identity: range2439971
display id: range2439971
version: 1
direction: direction/forward
top level: BBa_K1758332
start: 1
end: 68
orientation: inline

BBa_K824009
type: ComponentDefinition
persistent identity: BBa K824009
display id: BBa_K824009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Arsenic Promoter with GFP Reporter (Arsenic Detector)
creator: Steven Allen
created: 2012-10-01T11:00:00Z
modified: 2015-07-24T01:19:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1082_
m user id: 4206
owner id: 9218
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: We digested the promoter responsive to Arsenic(BBa_K174015) with EcoRI and SpeI and digested the GFP plasmid (BBa_I13401) with XbaI and PstI. The promoter was Chloramphenicol resistant and the GFP was Ampicillin resistant. These digested fragments were mixed and ligated to the provided, linearized pSB3C1 plasmid. The ligation mix was grown under Chloramphenicol selection. The resulting colonies were tested for responsive GFP production after the addition of Cadmium Chloride since the promoter is responsive to multiple heavy metals.
mutable notes: none
mutable provenance: This is a composite part formed from parts BBA_K174015 and BBa_I13401.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J33201
component: BBa_I13401
sequence annotation: BBa_I13401
sequence annotation: BBa_J33201
sequence: https://synbiohub.org/public/igem/BBa_K824009_sequence/1

BBa_J33201
type: Component
persistent identity: component2207586
display id: component2207586
version: 1
top level: BBa_K824009
access: public
definition: https://synbiohub.org/public/igem/BBa_J33201/1

BBa_I13401
type: Component
persistent identity: component2207594
display id: component2207594
version: 1
top level: BBa_K824009
access: public
definition: https://synbiohub.org/public/igem/BBa_I13401/1

BBa_I13401
type: SequenceAnnotation
persistent identity: annotation2207594
display id: annotation2207594
version: 1
top level: BBa_K824009
location: https://synbiohub.org/public/igem/BBa_K824009/annotation2207594/range2207594/1
component: BBa_I13401

https://synbiohub.org/public/igem/BBa_K824009/annotation2207594/range2207594/1
type: Range
persistent identity: range2207594
display id: range2207594
version: 1
direction: direction/reverse
top level: BBa_K824009
start: 525
end: 1381
orientation: inline

BBa_J33201
type: SequenceAnnotation
persistent identity: annotation2207586
display id: annotation2207586
version: 1
top level: BBa_K824009
location: https://synbiohub.org/public/igem/BBa_K824009/annotation2207586/range2207586/1
component: BBa_J33201

https://synbiohub.org/public/igem/BBa_K824009/annotation2207586/range2207586/1
type: Range
persistent identity: range2207586
display id: range2207586
version: 1
direction: direction/reverse
top level: BBa_K824009
start: 1
end: 518
orientation: inline

BBa_K1758352
type: ComponentDefinition
persistent identity: BBa K1758352
display id: BBa_K1758352
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Nickel responsive promoter with UTR-sfGFP
creator: Team Bielefeld-CeBiTec 2015
created: 2015-08-29T11:00:00Z
modified: 2016-01-06T03:38:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2180_
m user id: 4206
owner id: 26630
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Nickel respnsive promoter with 5?? untranslated region and sfGFP for detection
mutable notes: ...
mutable provenance: based on K549001
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: RcnA
sequence annotation: T7-term
sequence annotation: T1
sequence annotation: 5??UTR
sequence annotation: sfGFP
sequence: https://synbiohub.org/public/igem/BBa_K1758352_sequence/1

RcnA
type: SequenceAnnotation
persistent identity: annotation2440057
display id: annotation2440057
version: 1
top level: BBa_K1758352
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1758352/annotation2440057/range2440057/1

https://synbiohub.org/public/igem/BBa_K1758352/annotation2440057/range2440057/1
type: Range
persistent identity: range2440057
display id: range2440057
version: 1
direction: direction/forward
top level: BBa_K1758352
start: 1
end: 104
orientation: inline

T7-term
type: SequenceAnnotation
persistent identity: annotation2440063
display id: annotation2440063
version: 1
top level: BBa_K1758352
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1758352/annotation2440063/range2440063/1

https://synbiohub.org/public/igem/BBa_K1758352/annotation2440063/range2440063/1
type: Range
persistent identity: range2440063
display id: range2440063
version: 1
direction: direction/forward
top level: BBa_K1758352
start: 973
end: 1013
orientation: inline

T1
type: SequenceAnnotation
persistent identity: annotation2440062
display id: annotation2440062
version: 1
top level: BBa_K1758352
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1758352/annotation2440062/range2440062/1

https://synbiohub.org/public/igem/BBa_K1758352/annotation2440062/range2440062/1
type: Range
persistent identity: range2440062
display id: range2440062
version: 1
direction: direction/forward
top level: BBa_K1758352
start: 885
end: 964
orientation: inline

5??UTR
type: SequenceAnnotation
persistent identity: annotation2440058
display id: annotation2440058
version: 1
top level: BBa_K1758352
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1758352/annotation2440058/range2440058/1

https://synbiohub.org/public/igem/BBa_K1758352/annotation2440058/range2440058/1
type: Range
persistent identity: range2440058
display id: range2440058
version: 1
direction: direction/forward
top level: BBa_K1758352
start: 111
end: 156
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2440061
display id: annotation2440061
version: 1
top level: BBa_K1758352
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1758352/annotation2440061/range2440061/1

https://synbiohub.org/public/igem/BBa_K1758352/annotation2440061/range2440061/1
type: Range
persistent identity: range2440061
display id: range2440061
version: 1
direction: direction/forward
top level: BBa_K1758352
start: 157
end: 876
orientation: inline

BBa_K1980010
type: ComponentDefinition
persistent identity: BBa K1980010
display id: BBa_K1980010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCopA TAT Csp1 sfGFP with divergent CueR
creator: Sam Garforth
created: 2016-10-10T11:00:00Z
modified: 2016-10-24T08:12:51Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _2447_
m user id: 29607
owner id: 29607
owning group id: 9
status: status/Planning
bookmark: false
mutable description: pCopA TAT Csp1 sfGFP with plasmid constitutively expressed CueR
mutable notes: aahahahah
mutable provenance: E. coli, methanoba trcol OB3b
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
component: BBa_K1980001
component: BBa_K1980006
sequence annotation: BBa_K1980006
sequence annotation: BBa_K1980001
sequence: https://synbiohub.org/public/igem/BBa_K1980010_sequence/1

BBa_K1980001
type: Component
persistent identity: component2493042
display id: component2493042
version: 1
top level: BBa_K1980010
access: public
definition: https://synbiohub.org/public/igem/BBa_K1980001/1

BBa_K1980006
type: Component
persistent identity: component2493041
display id: component2493041
version: 1
top level: BBa_K1980010
access: public
definition: BBa_K1980006

BBa_K1980006
type: SequenceAnnotation
persistent identity: annotation2493041
display id: annotation2493041
version: 1
top level: BBa_K1980010
location: https://synbiohub.org/public/igem/BBa_K1980010/annotation2493041/range2493041/1
component: BBa_K1980006

https://synbiohub.org/public/igem/BBa_K1980010/annotation2493041/range2493041/1
type: Range
persistent identity: range2493041
display id: range2493041
version: 1
direction: direction/reverse
top level: BBa_K1980010
start: 1
end: 574
orientation: inline

BBa_K1980001
type: SequenceAnnotation
persistent identity: annotation2493042
display id: annotation2493042
version: 1
top level: BBa_K1980010
location: https://synbiohub.org/public/igem/BBa_K1980010/annotation2493042/range2493042/1
component: BBa_K1980001

https://synbiohub.org/public/igem/BBa_K1980010/annotation2493042/range2493042/1
type: Range
persistent identity: range2493042
display id: range2493042
version: 1
direction: direction/reverse
top level: BBa_K1980010
start: 581
end: 1783
orientation: inline

BBa_K1980006
type: ComponentDefinition
persistent identity: BBa K1980006
display id: BBa_K1980006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCopA with divergent expressed CueR
creator: Sam Garforth
created: 2016-10-10T11:00:00Z
modified: 2016-10-23T04:58:33Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2447_
m user id: 29607
owner id: 29607
owning group id: 9
status: status/Planning
bookmark: false
mutable description: pCopA with plasmid constitutively expressed CueR (no reporter)
mutable notes: Codon optimised. Designed for biobrick assembly with reporter of choice
mutable provenance: E. coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: RBS
sequence annotation: Double stop
sequence annotation: pCopA
sequence annotation: Constitutive promoter
sequence annotation: CueR
sequence: https://synbiohub.org/public/igem/BBa_K1980006_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2495263
display id: annotation2495263
version: 1
top level: BBa_K1980006
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1980006/annotation2495263/range2495263/1

https://synbiohub.org/public/igem/BBa_K1980006/annotation2495263/range2495263/1
type: Range
persistent identity: range2495263
display id: range2495263
version: 1
direction: direction/reverse
top level: BBa_K1980006
start: 418
end: 429
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2495260
display id: annotation2495260
version: 1
top level: BBa_K1980006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1980006/annotation2495260/range2495260/1

https://synbiohub.org/public/igem/BBa_K1980006/annotation2495260/range2495260/1
type: Range
persistent identity: range2495260
display id: range2495260
version: 1
direction: direction/forward
top level: BBa_K1980006
start: 563
end: 574
orientation: inline

Double stop
type: SequenceAnnotation
persistent identity: annotation2495261
display id: annotation2495261
version: 1
top level: BBa_K1980006
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1980006/annotation2495261/range2495261/1

https://synbiohub.org/public/igem/BBa_K1980006/annotation2495261/range2495261/1
type: Range
persistent identity: range2495261
display id: range2495261
version: 1
direction: direction/reverse
top level: BBa_K1980006
start: 1
end: 7
orientation: inline

pCopA
type: SequenceAnnotation
persistent identity: annotation2495265
display id: annotation2495265
version: 1
top level: BBa_K1980006
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1980006/annotation2495265/range2495265/1

https://synbiohub.org/public/igem/BBa_K1980006/annotation2495265/range2495265/1
type: Range
persistent identity: range2495265
display id: range2495265
version: 1
direction: direction/forward
top level: BBa_K1980006
start: 506
end: 556
orientation: inline

Constitutive promoter
type: SequenceAnnotation
persistent identity: annotation2495264
display id: annotation2495264
version: 1
top level: BBa_K1980006
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1980006/annotation2495264/range2495264/1

https://synbiohub.org/public/igem/BBa_K1980006/annotation2495264/range2495264/1
type: Range
persistent identity: range2495264
display id: range2495264
version: 1
direction: direction/reverse
top level: BBa_K1980006
start: 439
end: 472
orientation: inline

CueR
type: SequenceAnnotation
persistent identity: annotation2495262
display id: annotation2495262
version: 1
top level: BBa_K1980006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1980006/annotation2495262/range2495262/1

https://synbiohub.org/public/igem/BBa_K1980006/annotation2495262/range2495262/1
type: Range
persistent identity: range2495262
display id: range2495262
version: 1
direction: direction/reverse
top level: BBa_K1980006
start: 7
end: 411
orientation: inline

BBa_K1755301
type: ComponentDefinition
persistent identity: BBa K1755301
display id: BBa_K1755301
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: copper promoter + RBS + ribB + terminator
creator: Haotian Wang
created: 2015-04-19T11:00:00Z
modified: 2016-01-11T02:03:04Z
discontinued: false
dominant: false
group u list: _2177_
m user id: 4206
owner id: 21246
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: K190017 + k1755002
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0010
component: BBa_K190017
component: BBa_K1755002
component: BBa_B0012
sequence annotation: BBa_K190017
sequence annotation: BBa_B0010
sequence annotation: BBa_K1755002
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K1755301_sequence/1

BBa_B0010
type: Component
persistent identity: component2431281
display id: component2431281
version: 1
top level: BBa_K1755301
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_K190017
type: Component
persistent identity: component2431279
display id: component2431279
version: 1
top level: BBa_K1755301
access: public
definition: https://synbiohub.org/public/igem/BBa_K190017/1

BBa_K1755002
type: Component
persistent identity: component2431288
display id: component2431288
version: 1
top level: BBa_K1755301
access: public
definition: https://synbiohub.org/public/igem/BBa_K1755002/1

BBa_B0012
type: Component
persistent identity: component2431284
display id: component2431284
version: 1
top level: BBa_K1755301
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_K190017
type: SequenceAnnotation
persistent identity: annotation2431279
display id: annotation2431279
version: 1
top level: BBa_K1755301
location: https://synbiohub.org/public/igem/BBa_K1755301/annotation2431279/range2431279/1
component: BBa_K190017

https://synbiohub.org/public/igem/BBa_K1755301/annotation2431279/range2431279/1
type: Range
persistent identity: range2431279
display id: range2431279
version: 1
direction: direction/reverse
top level: BBa_K1755301
start: 1
end: 43
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2431281
display id: annotation2431281
version: 1
top level: BBa_K1755301
location: https://synbiohub.org/public/igem/BBa_K1755301/annotation2431281/range2431281/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K1755301/annotation2431281/range2431281/1
type: Range
persistent identity: range2431281
display id: range2431281
version: 1
direction: direction/forward
top level: BBa_K1755301
start: 712
end: 791
orientation: inline

BBa_K1755002
type: SequenceAnnotation
persistent identity: annotation2431288
display id: annotation2431288
version: 1
top level: BBa_K1755301
location: https://synbiohub.org/public/igem/BBa_K1755301/annotation2431288/range2431288/1
component: BBa_K1755002

https://synbiohub.org/public/igem/BBa_K1755301/annotation2431288/range2431288/1
type: Range
persistent identity: range2431288
display id: range2431288
version: 1
direction: direction/reverse
top level: BBa_K1755301
start: 50
end: 840
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2431284
display id: annotation2431284
version: 1
top level: BBa_K1755301
location: https://synbiohub.org/public/igem/BBa_K1755301/annotation2431284/range2431284/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K1755301/annotation2431284/range2431284/1
type: Range
persistent identity: range2431284
display id: range2431284
version: 1
direction: direction/forward
top level: BBa_K1755301
start: 800
end: 840
orientation: inline

PrFe
type: ComponentDefinition
persistent identity: BBa J3902
display id: BBa_J3902
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PrFe (PI + PII rus operon)
creator: Latin American iGEM team
created: 2006-10-28T11:00:00Z
modified: 2015-08-31T04:08:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _67_
m user id: 0
owner id: 1000
owning group id: 67
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Promoter region from Acidithiobacillus ferrooxidans rus operon
mutable notes: None
mutable provenance: Sequence found at NCBI (AJ006456)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: promoter
sequence: https://synbiohub.org/public/igem/BBa_J3902_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation1908375
display id: annotation1908375
version: 1
top level: PrFe
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J3902/annotation1908375/range1908375/1

https://synbiohub.org/public/igem/BBa_J3902/annotation1908375/range1908375/1
type: Range
persistent identity: range1908375
display id: range1908375
version: 1
direction: direction/forward
top level: PrFe
start: 7
end: 266
orientation: inline

BBa_K1980009
type: ComponentDefinition
persistent identity: BBa K1980009
display id: BBa_K1980009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCusC CusR RFP
creator: Sam Garforth
created: 2016-10-10T11:00:00Z
modified: 2016-10-23T07:39:56Z
discontinued: false
dominant: false
group u list: _2447_
m user id: 29607
owner id: 29607
owning group id: 9
status: status/Planning
bookmark: false
mutable description: pCusC CusR RFP
mutable notes: Stuff.
mutable provenance: E. coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: -35
sequence annotation: RBS
sequence annotation: t to c mutation (val to Ala)
sequence annotation: mKate2
sequence annotation: -10
sequence annotation: pCusC
sequence annotation: CusR
sequence annotation: Hexahistidine
sequence: https://synbiohub.org/public/igem/BBa_K1980009_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation2525373
display id: annotation2525373
version: 1
top level: BBa_K1980009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1980009/annotation2525373/range2525373/1

https://synbiohub.org/public/igem/BBa_K1980009/annotation2525373/range2525373/1
type: Range
persistent identity: range2525373
display id: range2525373
version: 1
direction: direction/forward
top level: BBa_K1980009
start: 60
end: 65
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2525372
display id: annotation2525372
version: 1
top level: BBa_K1980009
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1980009/annotation2525372/range2525372/1

https://synbiohub.org/public/igem/BBa_K1980009/annotation2525372/range2525372/1
type: Range
persistent identity: range2525372
display id: range2525372
version: 1
direction: direction/forward
top level: BBa_K1980009
start: 105
end: 116
orientation: inline

t to c mutation (val to Ala)
type: SequenceAnnotation
persistent identity: annotation2525368
display id: annotation2525368
version: 1
top level: BBa_K1980009
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1980009/annotation2525368/range2525368/1

https://synbiohub.org/public/igem/BBa_K1980009/annotation2525368/range2525368/1
type: Range
persistent identity: range2525368
display id: range2525368
version: 1
direction: direction/forward
top level: BBa_K1980009
start: 706
end: 706
orientation: inline

mKate2
type: SequenceAnnotation
persistent identity: annotation2525378
display id: annotation2525378
version: 1
top level: BBa_K1980009
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1980009/annotation2525378/range2525378/1

https://synbiohub.org/public/igem/BBa_K1980009/annotation2525378/range2525378/1
type: Range
persistent identity: range2525378
display id: range2525378
version: 1
direction: direction/forward
top level: BBa_K1980009
start: 838
end: 1530
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2525374
display id: annotation2525374
version: 1
top level: BBa_K1980009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1980009/annotation2525374/range2525374/1

https://synbiohub.org/public/igem/BBa_K1980009/annotation2525374/range2525374/1
type: Range
persistent identity: range2525374
display id: range2525374
version: 1
direction: direction/forward
top level: BBa_K1980009
start: 81
end: 87
orientation: inline

pCusC
type: SequenceAnnotation
persistent identity: annotation2525371
display id: annotation2525371
version: 1
top level: BBa_K1980009
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1980009/annotation2525371/range2525371/1

https://synbiohub.org/public/igem/BBa_K1980009/annotation2525371/range2525371/1
type: Range
persistent identity: range2525371
display id: range2525371
version: 1
direction: direction/forward
top level: BBa_K1980009
start: 1
end: 98
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2525377
display id: annotation2525377
version: 1
top level: BBa_K1980009
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1980009/annotation2525377/range2525377/1

https://synbiohub.org/public/igem/BBa_K1980009/annotation2525377/range2525377/1
type: Range
persistent identity: range2525377
display id: range2525377
version: 1
direction: direction/forward
top level: BBa_K1980009
start: 822
end: 827
orientation: inline

CusR
type: SequenceAnnotation
persistent identity: annotation2525375
display id: annotation2525375
version: 1
top level: BBa_K1980009
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1980009/annotation2525375/range2525375/1

https://synbiohub.org/public/igem/BBa_K1980009/annotation2525375/range2525375/1
type: Range
persistent identity: range2525375
display id: range2525375
version: 1
direction: direction/forward
top level: BBa_K1980009
start: 123
end: 809
orientation: inline

Hexahistidine
type: SequenceAnnotation
persistent identity: annotation2525379
display id: annotation2525379
version: 1
top level: BBa_K1980009
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1980009/annotation2525379/range2525379/1

https://synbiohub.org/public/igem/BBa_K1980009/annotation2525379/range2525379/1
type: Range
persistent identity: range2525379
display id: range2525379
version: 1
direction: direction/forward
top level: BBa_K1980009
start: 1531
end: 1554
orientation: inline

BBa_K1163110
type: ComponentDefinition
persistent identity: BBa K1163110
display id: BBa_K1163110
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: YncE promoter region
creator: Emiel van der Kouwe
created: 2013-09-21T11:00:00Z
modified: 2015-08-03T03:12:41Z
discontinued: false
dominant: false
group u list: _1475_
m user id: 4206
owner id: 14862
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoter from E. coli that controls the YncE gene, involved in iron uptake and iron sensitive. FUR (Ferric Uptake Regulation) is a transcriptional repressor of genes involved in iron homeostasis. In presence of iron, the FUR protein changes its conformation and dimerizes. This leads to the binding to the DNA on a FUR binding site, thus inhibiting the mRNA transcription. Why would you use this part? This promoter allows you to have an iron-sensitive behaviour. In fact, in the presence of iron, the YncE promoter region decreases the expression of the downstream gene.
mutable notes: We blindly extracted the 300 bp sequence upstream of the YncE gene in E. coli. It contains a putative FUR binding site and a putative RBS.
mutable provenance: Genomic DNA.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: YncE
sequence: https://synbiohub.org/public/igem/BBa_K1163110_sequence/1

YncE
type: SequenceAnnotation
persistent identity: annotation2358164
display id: annotation2358164
version: 1
top level: BBa_K1163110
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1163110/annotation2358164/range2358164/1

https://synbiohub.org/public/igem/BBa_K1163110/annotation2358164/range2358164/1
type: Range
persistent identity: range2358164
display id: range2358164
version: 1
direction: direction/forward
top level: BBa_K1163110
start: 1
end: 300
orientation: inline

BBa_I760005
type: ComponentDefinition
persistent identity: BBa I760005
display id: BBa_I760005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cu-sensitive promoter
creator: Gennady Zakharov
created: 2007-08-19T11:00:00Z
modified: 2015-08-31T04:08:08Z
discontinued: false
dominant: false
experience: experience/None
group u list: _148_
m user id: 0
owner id: 2080
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoter from the copper-sensetive CusR/CusS two component signal system. (E.Coli)
mutable notes: Sequence from the NCBI database, with added BioBricks prefix and suffix.
mutable provenance: http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I760005_sequence/1

BBa_K1724000
type: ComponentDefinition
persistent identity: BBa K1724000
display id: BBa_K1724000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pcada
creator: Zhanru Chen
created: 2015-09-04T11:00:00Z
modified: 2016-01-11T03:19:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2144_
m user id: 4206
owner id: 21980
owning group id: 9
status: status/Planning
bookmark: true
mutable description: cada promoter
mutable notes: FP:GTTTCTTCGAATTCGCGGCCGCTTCTAGATTGACTCTGTAGTTGCTACA VP:GTTTCTTCCTGCAGCGGCCGCTACTAGT ATTGCACACC CTGTAGCAAC TACAGA
mutable provenance: OVERLAP PCR
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: cadmium activated promoter
sequence: https://synbiohub.org/public/igem/BBa_K1724000_sequence/1

cadmium activated promoter
type: SequenceAnnotation
persistent identity: annotation2443820
display id: annotation2443820
version: 1
top level: BBa_K1724000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1724000/annotation2443820/range2443820/1

https://synbiohub.org/public/igem/BBa_K1724000/annotation2443820/range2443820/1
type: Range
persistent identity: range2443820
display id: range2443820
version: 1
direction: direction/forward
top level: BBa_K1724000
start: 1
end: 31
orientation: inline

BBa_K1758342
type: ComponentDefinition
persistent identity: BBa K1758342
display id: BBa_K1758342
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mercury responsive promoter with UTR-sfGFP
creator: Team Bielefeld-CeBiTec 2015
created: 2015-08-28T11:00:00Z
modified: 2016-01-06T03:41:22Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2180_
m user id: 4206
owner id: 26630
owning group id: 9
status: status/Planning
bookmark: false
mutable description: mercury responsive promoter with 5??untranslated region and sfGFP for detection
mutable notes: ...
mutable provenance: modified after K346002
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: T7-term
sequence annotation: sfGFP
sequence annotation: 5??UTR
sequence annotation: merT
sequence annotation: T1
sequence: https://synbiohub.org/public/igem/BBa_K1758342_sequence/1

T7-term
type: SequenceAnnotation
persistent identity: annotation2439970
display id: annotation2439970
version: 1
top level: BBa_K1758342
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1758342/annotation2439970/range2439970/1

https://synbiohub.org/public/igem/BBa_K1758342/annotation2439970/range2439970/1
type: Range
persistent identity: range2439970
display id: range2439970
version: 1
direction: direction/forward
top level: BBa_K1758342
start: 926
end: 966
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2439968
display id: annotation2439968
version: 1
top level: BBa_K1758342
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1758342/annotation2439968/range2439968/1

https://synbiohub.org/public/igem/BBa_K1758342/annotation2439968/range2439968/1
type: Range
persistent identity: range2439968
display id: range2439968
version: 1
direction: direction/forward
top level: BBa_K1758342
start: 110
end: 829
orientation: inline

5??UTR
type: SequenceAnnotation
persistent identity: annotation2439967
display id: annotation2439967
version: 1
top level: BBa_K1758342
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1758342/annotation2439967/range2439967/1

https://synbiohub.org/public/igem/BBa_K1758342/annotation2439967/range2439967/1
type: Range
persistent identity: range2439967
display id: range2439967
version: 1
direction: direction/forward
top level: BBa_K1758342
start: 64
end: 109
orientation: inline

merT
type: SequenceAnnotation
persistent identity: annotation2439966
display id: annotation2439966
version: 1
top level: BBa_K1758342
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1758342/annotation2439966/range2439966/1

https://synbiohub.org/public/igem/BBa_K1758342/annotation2439966/range2439966/1
type: Range
persistent identity: range2439966
display id: range2439966
version: 1
direction: direction/forward
top level: BBa_K1758342
start: 1
end: 57
orientation: inline

T1
type: SequenceAnnotation
persistent identity: annotation2439969
display id: annotation2439969
version: 1
top level: BBa_K1758342
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1758342/annotation2439969/range2439969/1

https://synbiohub.org/public/igem/BBa_K1758342/annotation2439969/range2439969/1
type: Range
persistent identity: range2439969
display id: range2439969
version: 1
direction: direction/forward
top level: BBa_K1758342
start: 838
end: 917
orientation: inline

zinTp
type: ComponentDefinition
persistent identity: BBa K896008
display id: BBa_K896008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: zinTp (Cd2+ sensing promoter)
creator: Shih-Hao Chen
created: 2012-09-21T11:00:00Z
modified: 2015-07-24T01:12:28Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1161_
m user id: 4206
owner id: 9030
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: YES
mutable notes: YES
mutable provenance: YES
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: promoter
sequence: https://synbiohub.org/public/igem/BBa_K896008_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation2202313
display id: annotation2202313
version: 1
top level: zinTp
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K896008/annotation2202313/range2202313/1

https://synbiohub.org/public/igem/BBa_K896008/annotation2202313/range2202313/1
type: Range
persistent identity: range2202313
display id: range2202313
version: 1
direction: direction/forward
top level: zinTp
start: 1
end: 81
orientation: inline

BBa_K1758344
type: ComponentDefinition
persistent identity: BBa K1758344
display id: BBa_K1758344
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mercury responsive promoter with T7-promoter and UTR-sfGFP
creator: Team Bielefeld-CeBiTec 2015
created: 2015-08-28T11:00:00Z
modified: 2016-01-06T03:38:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2180_
m user id: 4206
owner id: 26630
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Mercury responsive Promoter with 5?? untranslated region and superfolding GFP
mutable notes: ---
mutable provenance: modified after BBaK346002
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: merT
sequence annotation: T7
sequence annotation: sfGFP
sequence annotation: T7-term
sequence annotation: T1
sequence: https://synbiohub.org/public/igem/BBa_K1758344_sequence/1

merT
type: SequenceAnnotation
persistent identity: annotation2439951
display id: annotation2439951
version: 1
top level: BBa_K1758344
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1758344/annotation2439951/range2439951/1

https://synbiohub.org/public/igem/BBa_K1758344/annotation2439951/range2439951/1
type: Range
persistent identity: range2439951
display id: range2439951
version: 1
direction: direction/forward
top level: BBa_K1758344
start: 24
end: 80
orientation: inline

T7
type: SequenceAnnotation
persistent identity: annotation2439950
display id: annotation2439950
version: 1
top level: BBa_K1758344
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1758344/annotation2439950/range2439950/1

https://synbiohub.org/public/igem/BBa_K1758344/annotation2439950/range2439950/1
type: Range
persistent identity: range2439950
display id: range2439950
version: 1
direction: direction/forward
top level: BBa_K1758344
start: 1
end: 23
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2439952
display id: annotation2439952
version: 1
top level: BBa_K1758344
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1758344/annotation2439952/range2439952/1

https://synbiohub.org/public/igem/BBa_K1758344/annotation2439952/range2439952/1
type: Range
persistent identity: range2439952
display id: range2439952
version: 1
direction: direction/forward
top level: BBa_K1758344
start: 133
end: 852
orientation: inline

T7-term
type: SequenceAnnotation
persistent identity: annotation2439954
display id: annotation2439954
version: 1
top level: BBa_K1758344
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1758344/annotation2439954/range2439954/1

https://synbiohub.org/public/igem/BBa_K1758344/annotation2439954/range2439954/1
type: Range
persistent identity: range2439954
display id: range2439954
version: 1
direction: direction/forward
top level: BBa_K1758344
start: 949
end: 989
orientation: inline

T1
type: SequenceAnnotation
persistent identity: annotation2439953
display id: annotation2439953
version: 1
top level: BBa_K1758344
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1758344/annotation2439953/range2439953/1

https://synbiohub.org/public/igem/BBa_K1758344/annotation2439953/range2439953/1
type: Range
persistent identity: range2439953
display id: range2439953
version: 1
direction: direction/forward
top level: BBa_K1758344
start: 861
end: 940
orientation: inline

BBa_I731004
type: ComponentDefinition
persistent identity: BBa I731004
display id: BBa_I731004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FecA promoter
creator: Shaunak Vankudre
created: 2007-06-25T11:00:00Z
modified: 2015-08-31T04:07:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _127_
m user id: 0
owner id: 1889
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: derived from Enz et al, 2003, J Bacteriol. 2003 April; 185(7): 2387???2391. doi: 10.1128/JB.185.7.2387-2391.2003.
mutable provenance: genomic sequence from E. Coli K12
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I731004_sequence/1

BBa_K824012
type: ComponentDefinition
persistent identity: BBa K824012
display id: BBa_K824012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lead Promoter with GFP Reporter (Lead Detector)
creator: Steven Allen
created: 2012-10-01T11:00:00Z
modified: 2015-07-24T01:20:04Z
discontinued: false
dominant: false
group u list: _1082_
m user id: 4206
owner id: 9218
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: We digested the promoter responsive to Lead (BBa_I721001) with EcoRI and SpeI and digested the GFP plasmid (BBa_I13401) with XbaI and PstI. The promoter and reporter were Ampicillin resistant. These digested fragments were mixed and ligated to the provided, linearized pSB1C3 plasmid. The ligation mix was grown under Chloramphenicol selection. The resulting colonies were tested for responsive GFP production following the addition of Lead Nitrate.
mutable notes: none
mutable provenance: The composite part was formed from BBa_I721001 and BBa_I13401.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I721001
component: BBa_I13401
sequence annotation: BBa_I721001
sequence annotation: BBa_I13401
sequence: https://synbiohub.org/public/igem/BBa_K824012_sequence/1

BBa_I721001
type: Component
persistent identity: component2206435
display id: component2206435
version: 1
top level: BBa_K824012
access: public
definition: BBa_I721001

BBa_I13401
type: Component
persistent identity: component2206443
display id: component2206443
version: 1
top level: BBa_K824012
access: public
definition: https://synbiohub.org/public/igem/BBa_I13401/1

BBa_I721001
type: SequenceAnnotation
persistent identity: annotation2206435
display id: annotation2206435
version: 1
top level: BBa_K824012
location: https://synbiohub.org/public/igem/BBa_K824012/annotation2206435/range2206435/1
component: BBa_I721001

https://synbiohub.org/public/igem/BBa_K824012/annotation2206435/range2206435/1
type: Range
persistent identity: range2206435
display id: range2206435
version: 1
direction: direction/reverse
top level: BBa_K824012
start: 1
end: 94
orientation: inline

BBa_I13401
type: SequenceAnnotation
persistent identity: annotation2206443
display id: annotation2206443
version: 1
top level: BBa_K824012
location: https://synbiohub.org/public/igem/BBa_K824012/annotation2206443/range2206443/1
component: BBa_I13401

https://synbiohub.org/public/igem/BBa_K824012/annotation2206443/range2206443/1
type: Range
persistent identity: range2206443
display id: range2206443
version: 1
direction: direction/reverse
top level: BBa_K824012
start: 101
end: 957
orientation: inline

BBa_K1755302
type: ComponentDefinition
persistent identity: BBa K1755302
display id: BBa_K1755302
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: copper promoter + RBS + ribB + terminator
creator: Haotian Wang
created: 2015-04-19T11:00:00Z
modified: 2016-01-11T02:02:06Z
discontinued: false
dominant: false
group u list: _2177_
m user id: 4206
owner id: 21246
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: k190024 + K1755003
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_K190024
component: BBa_K1755001
component: BBa_B0034
component: BBa_K1755003
component: BBa_B0010
component: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_K190024
sequence annotation: BBa_K1755003
sequence annotation: BBa_K1755001
sequence: https://synbiohub.org/public/igem/BBa_K1755302_sequence/1

BBa_K190024
type: Component
persistent identity: component2431291
display id: component2431291
version: 1
top level: BBa_K1755302
access: public
definition: https://synbiohub.org/public/igem/BBa_K190024/1

BBa_K1755001
type: Component
persistent identity: component2431295
display id: component2431295
version: 1
top level: BBa_K1755302
access: public
definition: https://synbiohub.org/public/igem/BBa_K1755001/1

BBa_B0034
type: Component
persistent identity: component2431292
display id: component2431292
version: 1
top level: BBa_K1755302
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1755003
type: Component
persistent identity: component2431303
display id: component2431303
version: 1
top level: BBa_K1755302
access: public
definition: https://synbiohub.org/public/igem/BBa_K1755003/1

BBa_B0010
type: Component
persistent identity: component2431296
display id: component2431296
version: 1
top level: BBa_K1755302
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component2431299
display id: component2431299
version: 1
top level: BBa_K1755302
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2431296
display id: annotation2431296
version: 1
top level: BBa_K1755302
location: https://synbiohub.org/public/igem/BBa_K1755302/annotation2431296/range2431296/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K1755302/annotation2431296/range2431296/1
type: Range
persistent identity: range2431296
display id: range2431296
version: 1
direction: direction/forward
top level: BBa_K1755302
start: 760
end: 839
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2431292
display id: annotation2431292
version: 1
top level: BBa_K1755302
location: https://synbiohub.org/public/igem/BBa_K1755302/annotation2431292/range2431292/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1755302/annotation2431292/range2431292/1
type: Range
persistent identity: range2431292
display id: range2431292
version: 1
direction: direction/reverse
top level: BBa_K1755302
start: 80
end: 91
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2431299
display id: annotation2431299
version: 1
top level: BBa_K1755302
location: https://synbiohub.org/public/igem/BBa_K1755302/annotation2431299/range2431299/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K1755302/annotation2431299/range2431299/1
type: Range
persistent identity: range2431299
display id: range2431299
version: 1
direction: direction/forward
top level: BBa_K1755302
start: 848
end: 888
orientation: inline

BBa_K190024
type: SequenceAnnotation
persistent identity: annotation2431291
display id: annotation2431291
version: 1
top level: BBa_K1755302
location: https://synbiohub.org/public/igem/BBa_K1755302/annotation2431291/range2431291/1
component: BBa_K190024

https://synbiohub.org/public/igem/BBa_K1755302/annotation2431291/range2431291/1
type: Range
persistent identity: range2431291
display id: range2431291
version: 1
direction: direction/reverse
top level: BBa_K1755302
start: 1
end: 71
orientation: inline

BBa_K1755003
type: SequenceAnnotation
persistent identity: annotation2431303
display id: annotation2431303
version: 1
top level: BBa_K1755302
location: https://synbiohub.org/public/igem/BBa_K1755302/annotation2431303/range2431303/1
component: BBa_K1755003

https://synbiohub.org/public/igem/BBa_K1755302/annotation2431303/range2431303/1
type: Range
persistent identity: range2431303
display id: range2431303
version: 1
direction: direction/reverse
top level: BBa_K1755302
start: 80
end: 888
orientation: inline

BBa_K1755001
type: SequenceAnnotation
persistent identity: annotation2431295
display id: annotation2431295
version: 1
top level: BBa_K1755302
location: https://synbiohub.org/public/igem/BBa_K1755302/annotation2431295/range2431295/1
component: BBa_K1755001

https://synbiohub.org/public/igem/BBa_K1755302/annotation2431295/range2431295/1
type: Range
persistent identity: range2431295
display id: range2431295
version: 1
direction: direction/reverse
top level: BBa_K1755302
start: 98
end: 751
orientation: inline

BBa_K1758334
type: ComponentDefinition
persistent identity: BBa K1758334
display id: BBa_K1758334
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lead responsive promoter with T7-promoter and UTR-sfGFP
creator: Team Bielefeld-CeBiTec 2015
created: 2015-09-07T11:00:00Z
modified: 2016-01-06T03:41:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2180_
m user id: 4206
owner id: 26630
owning group id: 9
status: status/Planning
bookmark: false
mutable description: ...
mutable notes: ..
mutable provenance: ...
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1758332
component: BBa_I719005
sequence annotation: BBa_I719005
sequence annotation: BBa_K1758332
sequence: https://synbiohub.org/public/igem/BBa_K1758334_sequence/1

BBa_K1758332
type: Component
persistent identity: component2447495
display id: component2447495
version: 1
top level: BBa_K1758334
access: public
definition: BBa_K1758332

BBa_I719005
type: Component
persistent identity: component2447489
display id: component2447489
version: 1
top level: BBa_K1758334
access: public
definition: https://synbiohub.org/public/igem/BBa_I719005/1

BBa_I719005
type: SequenceAnnotation
persistent identity: annotation2447489
display id: annotation2447489
version: 1
top level: BBa_K1758334
location: https://synbiohub.org/public/igem/BBa_K1758334/annotation2447489/range2447489/1
component: BBa_I719005

https://synbiohub.org/public/igem/BBa_K1758334/annotation2447489/range2447489/1
type: Range
persistent identity: range2447489
display id: range2447489
version: 1
direction: direction/reverse
top level: BBa_K1758334
start: 1
end: 23
orientation: inline

BBa_K1758332
type: SequenceAnnotation
persistent identity: annotation2447495
display id: annotation2447495
version: 1
top level: BBa_K1758334
location: https://synbiohub.org/public/igem/BBa_K1758334/annotation2447495/range2447495/1
component: BBa_K1758332

https://synbiohub.org/public/igem/BBa_K1758334/annotation2447495/range2447495/1
type: Range
persistent identity: range2447495
display id: range2447495
version: 1
direction: direction/reverse
top level: BBa_K1758334
start: 32
end: 1008
orientation: inline

BBa_K1755305
type: ComponentDefinition
persistent identity: BBa K1755305
display id: BBa_K1755305
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Hg Sensor
creator: Haotian Wang
created: 2015-05-31T11:00:00Z
modified: 2016-01-11T02:02:39Z
discontinued: false
dominant: false
group u list: _2177_
m user id: 4206
owner id: 21246
owning group id: 9
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_K346002
component: BBa_K1755003
sequence annotation: BBa_K1755003
sequence annotation: BBa_K346002
sequence: https://synbiohub.org/public/igem/BBa_K1755305_sequence/1

BBa_K346002
type: Component
persistent identity: component2432465
display id: component2432465
version: 1
top level: BBa_K1755305
access: public
definition: BBa_K346002

BBa_K1755003
type: Component
persistent identity: component2432478
display id: component2432478
version: 1
top level: BBa_K1755305
access: public
definition: https://synbiohub.org/public/igem/BBa_K1755003/1

BBa_K1755003
type: SequenceAnnotation
persistent identity: annotation2432478
display id: annotation2432478
version: 1
top level: BBa_K1755305
location: https://synbiohub.org/public/igem/BBa_K1755305/annotation2432478/range2432478/1
component: BBa_K1755003

https://synbiohub.org/public/igem/BBa_K1755305/annotation2432478/range2432478/1
type: Range
persistent identity: range2432478
display id: range2432478
version: 1
direction: direction/reverse
top level: BBa_K1755305
start: 66
end: 841
orientation: inline

BBa_K346002
type: SequenceAnnotation
persistent identity: annotation2432465
display id: annotation2432465
version: 1
top level: BBa_K1755305
location: https://synbiohub.org/public/igem/BBa_K1755305/annotation2432465/range2432465/1
component: BBa_K346002

https://synbiohub.org/public/igem/BBa_K1755305/annotation2432465/range2432465/1
type: Range
persistent identity: range2432465
display id: range2432465
version: 1
direction: direction/reverse
top level: BBa_K1755305
start: 1
end: 57
orientation: inline

BBa_K1749000
type: ComponentDefinition
persistent identity: BBa K1749000
display id: BBa_K1749000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cd sensitive promoter with Phi delta activator, PO promoter, and GFP
creator: Anne Byford
created: 2015-09-16T11:00:00Z
modified: 2016-01-11T01:35:16Z
discontinued: false
dominant: false
group u list: _2171_
m user id: 4206
owner id: 4261
owning group id: 9
status: status/Planning
bookmark: false
mutable description: The Cadmium sensitive promoter is combined with amplifiers from the 2009 Cambridge iGEM team to increase the sensitivity to cadmium. It uses GFP as a reporter.
mutable notes: None.
mutable provenance: This composite part contains only other biobrick parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0010
component: BBa_E0040
component: BBa_K174017
component: BBa_I746352
component: BBa_K174016
component: BBa_B0012
component: BBa_I746361
sequence annotation: BBa_K174017
sequence annotation: BBa_E0040
sequence annotation: BBa_K174016
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_I746361
sequence annotation: BBa_I746352
sequence: https://synbiohub.org/public/igem/BBa_K1749000_sequence/1

BBa_B0010
type: Component
persistent identity: component2466671
display id: component2466671
version: 1
top level: BBa_K1749000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0040
type: Component
persistent identity: component2466670
display id: component2466670
version: 1
top level: BBa_K1749000
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_K174017
type: Component
persistent identity: component2466661
display id: component2466661
version: 1
top level: BBa_K1749000
access: public
definition: BBa_K174017

BBa_I746352
type: Component
persistent identity: component2466666
display id: component2466666
version: 1
top level: BBa_K1749000
access: public
definition: https://synbiohub.org/public/igem/BBa_I746352/1

BBa_K174016
type: Component
persistent identity: component2466656
display id: component2466656
version: 1
top level: BBa_K1749000
access: public
definition: BBa_K174016

BBa_B0012
type: Component
persistent identity: component2466673
display id: component2466673
version: 1
top level: BBa_K1749000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_I746361
type: Component
persistent identity: component2466668
display id: component2466668
version: 1
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definition: https://synbiohub.org/public/igem/BBa_I746361/1

BBa_K174017
type: SequenceAnnotation
persistent identity: annotation2466661
display id: annotation2466661
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top level: BBa_K1749000
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BBa_E0040
type: SequenceAnnotation
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component: BBa_E0040

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BBa_K174016
type: SequenceAnnotation
persistent identity: annotation2466656
display id: annotation2466656
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top level: BBa_K1749000
location: https://synbiohub.org/public/igem/BBa_K1749000/annotation2466656/range2466656/1
component: BBa_K174016

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type: Range
persistent identity: range2466656
display id: range2466656
version: 1
direction: direction/reverse
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start: 1
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BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2466671
display id: annotation2466671
version: 1
top level: BBa_K1749000
location: https://synbiohub.org/public/igem/BBa_K1749000/annotation2466671/range2466671/1
component: BBa_B0010

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persistent identity: range2466671
display id: range2466671
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start: 1312
end: 1391
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BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2466673
display id: annotation2466673
version: 1
top level: BBa_K1749000
location: https://synbiohub.org/public/igem/BBa_K1749000/annotation2466673/range2466673/1
component: BBa_B0012

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persistent identity: range2466673
display id: range2466673
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start: 1400
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BBa_I746361
type: SequenceAnnotation
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display id: annotation2466668
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top level: BBa_K1749000
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component: BBa_I746361

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display id: range2466668
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start: 486
end: 577
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BBa_I746352
type: SequenceAnnotation
persistent identity: annotation2466666
display id: annotation2466666
version: 1
top level: BBa_K1749000
location: https://synbiohub.org/public/igem/BBa_K1749000/annotation2466666/range2466666/1
component: BBa_I746352

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type: Range
persistent identity: range2466666
display id: range2466666
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end: 477
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BBa_K1755303
type: ComponentDefinition
persistent identity: BBa K1755303
display id: BBa_K1755303
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cobalt sensor
creator: Haotian Wang
created: 2015-04-19T11:00:00Z
modified: 2016-01-11T01:58:18Z
discontinued: false
dominant: false
group u list: _2177_
m user id: 4206
owner id: 21246
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: K540001 + K1755003
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_K540001
component: BBa_B0012
component: BBa_B0010
component: BBa_K1755001
component: BBa_K1755003
component: BBa_B0034
sequence annotation: BBa_K1755003
sequence annotation: BBa_K540001
sequence annotation: BBa_K1755001
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_K1755303_sequence/1

BBa_K540001
type: Component
persistent identity: component2431317
display id: component2431317
version: 1
top level: BBa_K1755303
access: public
definition: BBa_K540001

BBa_B0012
type: Component
persistent identity: component2431325
display id: component2431325
version: 1
top level: BBa_K1755303
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component2431322
display id: component2431322
version: 1
top level: BBa_K1755303
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_K1755001
type: Component
persistent identity: component2431321
display id: component2431321
version: 1
top level: BBa_K1755303
access: public
definition: https://synbiohub.org/public/igem/BBa_K1755001/1

BBa_K1755003
type: Component
persistent identity: component2431329
display id: component2431329
version: 1
top level: BBa_K1755303
access: public
definition: https://synbiohub.org/public/igem/BBa_K1755003/1

BBa_B0034
type: Component
persistent identity: component2431318
display id: component2431318
version: 1
top level: BBa_K1755303
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1755003
type: SequenceAnnotation
persistent identity: annotation2431329
display id: annotation2431329
version: 1
top level: BBa_K1755303
location: https://synbiohub.org/public/igem/BBa_K1755303/annotation2431329/range2431329/1
component: BBa_K1755003

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type: Range
persistent identity: range2431329
display id: range2431329
version: 1
direction: direction/reverse
top level: BBa_K1755303
start: 435
end: 1243
orientation: inline

BBa_K540001
type: SequenceAnnotation
persistent identity: annotation2431317
display id: annotation2431317
version: 1
top level: BBa_K1755303
location: https://synbiohub.org/public/igem/BBa_K1755303/annotation2431317/range2431317/1
component: BBa_K540001

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type: Range
persistent identity: range2431317
display id: range2431317
version: 1
direction: direction/reverse
top level: BBa_K1755303
start: 1
end: 426
orientation: inline

BBa_K1755001
type: SequenceAnnotation
persistent identity: annotation2431321
display id: annotation2431321
version: 1
top level: BBa_K1755303
location: https://synbiohub.org/public/igem/BBa_K1755303/annotation2431321/range2431321/1
component: BBa_K1755001

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type: Range
persistent identity: range2431321
display id: range2431321
version: 1
direction: direction/reverse
top level: BBa_K1755303
start: 453
end: 1106
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2431325
display id: annotation2431325
version: 1
top level: BBa_K1755303
location: https://synbiohub.org/public/igem/BBa_K1755303/annotation2431325/range2431325/1
component: BBa_B0012

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type: Range
persistent identity: range2431325
display id: range2431325
version: 1
direction: direction/forward
top level: BBa_K1755303
start: 1203
end: 1243
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2431318
display id: annotation2431318
version: 1
top level: BBa_K1755303
location: https://synbiohub.org/public/igem/BBa_K1755303/annotation2431318/range2431318/1
component: BBa_B0034

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type: Range
persistent identity: range2431318
display id: range2431318
version: 1
direction: direction/reverse
top level: BBa_K1755303
start: 435
end: 446
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2431322
display id: annotation2431322
version: 1
top level: BBa_K1755303
location: https://synbiohub.org/public/igem/BBa_K1755303/annotation2431322/range2431322/1
component: BBa_B0010

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type: Range
persistent identity: range2431322
display id: range2431322
version: 1
direction: direction/forward
top level: BBa_K1755303
start: 1115
end: 1194
orientation: inline

BBa_K1980012
type: ComponentDefinition
persistent identity: BBa K1980012
display id: BBa_K1980012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCopA MymT sfGFP with divergent CueR
creator: Sam Garforth
created: 2016-10-10T11:00:00Z
modified: 2016-10-23T07:47:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2447_
m user id: 0
m user id: 29607
owner id: 29607
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: pCopA MymT sfGFP with plasmid constitutively expressed CueR
mutable notes: dsjsdgydahjah
mutable provenance: E. coli TB
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K1980006
component: BBa_K1980003
sequence annotation: BBa_K1980006
sequence annotation: BBa_K1980003
sequence: https://synbiohub.org/public/igem/BBa_K1980012_sequence/1

BBa_K1980006
type: Component
persistent identity: component2493045
display id: component2493045
version: 1
top level: BBa_K1980012
access: public
definition: BBa_K1980006

BBa_K1980003
type: Component
persistent identity: component2493046
display id: component2493046
version: 1
top level: BBa_K1980012
access: public
definition: https://synbiohub.org/public/igem/BBa_K1980003/1

BBa_K1980006
type: SequenceAnnotation
persistent identity: annotation2493045
display id: annotation2493045
version: 1
top level: BBa_K1980012
location: https://synbiohub.org/public/igem/BBa_K1980012/annotation2493045/range2493045/1
component: BBa_K1980006

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type: Range
persistent identity: range2493045
display id: range2493045
version: 1
direction: direction/reverse
top level: BBa_K1980012
start: 1
end: 574
orientation: inline

BBa_K1980003
type: SequenceAnnotation
persistent identity: annotation2493046
display id: annotation2493046
version: 1
top level: BBa_K1980012
location: https://synbiohub.org/public/igem/BBa_K1980012/annotation2493046/range2493046/1
component: BBa_K1980003

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type: Range
persistent identity: range2493046
display id: range2493046
version: 1
direction: direction/reverse
top level: BBa_K1980012
start: 581
end: 1492
orientation: inline

BBa_K1758325
type: ComponentDefinition
persistent identity: BBa K1758325
display id: BBa_K1758325
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Copper responsive promoter with T7-promoter and UTR-sfGFP
creator: Team Bielefeld-CeBiTec 2015
created: 2015-09-07T11:00:00Z
modified: 2016-01-06T03:42:09Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2180_
m user id: 4206
owner id: 26630
owning group id: 9
status: status/Planning
bookmark: false
mutable description: ...
mutable notes: ...
mutable provenance: ...
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1758323
component: BBa_I719005
sequence annotation: BBa_K1758323
sequence annotation: BBa_I719005
sequence: https://synbiohub.org/public/igem/BBa_K1758325_sequence/1

BBa_K1758323
type: Component
persistent identity: component2447488
display id: component2447488
version: 1
top level: BBa_K1758325
access: public
definition: BBa_K1758323

BBa_I719005
type: Component
persistent identity: component2447482
display id: component2447482
version: 1
top level: BBa_K1758325
access: public
definition: https://synbiohub.org/public/igem/BBa_I719005/1

BBa_K1758323
type: SequenceAnnotation
persistent identity: annotation2447488
display id: annotation2447488
version: 1
top level: BBa_K1758325
location: https://synbiohub.org/public/igem/BBa_K1758325/annotation2447488/range2447488/1
component: BBa_K1758323

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type: Range
persistent identity: range2447488
display id: range2447488
version: 1
direction: direction/reverse
top level: BBa_K1758325
start: 32
end: 1023
orientation: inline

BBa_I719005
type: SequenceAnnotation
persistent identity: annotation2447482
display id: annotation2447482
version: 1
top level: BBa_K1758325
location: https://synbiohub.org/public/igem/BBa_K1758325/annotation2447482/range2447482/1
component: BBa_I719005

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type: Range
persistent identity: range2447482
display id: range2447482
version: 1
direction: direction/reverse
top level: BBa_K1758325
start: 1
end: 23
orientation: inline

BBa_K1758323
type: ComponentDefinition
persistent identity: BBa K1758323
display id: BBa_K1758323
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: UTR-sfGFP under control of Copper responsive promoter
creator: Team Bielefeld-CeBiTec 2015
created: 2015-08-28T11:00:00Z
modified: 2016-01-06T03:42:20Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2180_
m user id: 4206
owner id: 26630
owning group id: 9
status: status/Planning
bookmark: false
mutable description: copper responsive promoter with 5?? untranslated region and sfGFP for detection
mutable notes: ...
mutable provenance: copper responsive promoter of ????E.coli`` with sfGFP for detection
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: sfGFP
sequence annotation: 5??UTR
sequence annotation: CopAp
sequence annotation: T7-term
sequence annotation: T1
sequence: https://synbiohub.org/public/igem/BBa_K1758323_sequence/1

sfGFP
type: SequenceAnnotation
persistent identity: annotation2439963
display id: annotation2439963
version: 1
top level: BBa_K1758323
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1758323/annotation2439963/range2439963/1

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type: Range
persistent identity: range2439963
display id: range2439963
version: 1
direction: direction/forward
top level: BBa_K1758323
start: 136
end: 855
orientation: inline

5??UTR
type: SequenceAnnotation
persistent identity: annotation2439962
display id: annotation2439962
version: 1
top level: BBa_K1758323
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1758323/annotation2439962/range2439962/1

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type: Range
persistent identity: range2439962
display id: range2439962
version: 1
direction: direction/forward
top level: BBa_K1758323
start: 90
end: 135
orientation: inline

CopAp
type: SequenceAnnotation
persistent identity: annotation2439961
display id: annotation2439961
version: 1
top level: BBa_K1758323
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1758323/annotation2439961/range2439961/1

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type: Range
persistent identity: range2439961
display id: range2439961
version: 1
direction: direction/forward
top level: BBa_K1758323
start: 1
end: 83
orientation: inline

T7-term
type: SequenceAnnotation
persistent identity: annotation2439965
display id: annotation2439965
version: 1
top level: BBa_K1758323
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1758323/annotation2439965/range2439965/1

https://synbiohub.org/public/igem/BBa_K1758323/annotation2439965/range2439965/1
type: Range
persistent identity: range2439965
display id: range2439965
version: 1
direction: direction/forward
top level: BBa_K1758323
start: 952
end: 992
orientation: inline

T1
type: SequenceAnnotation
persistent identity: annotation2439964
display id: annotation2439964
version: 1
top level: BBa_K1758323
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1758323/annotation2439964/range2439964/1

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type: Range
persistent identity: range2439964
display id: range2439964
version: 1
direction: direction/forward
top level: BBa_K1758323
start: 864
end: 943
orientation: inline