//function/recombination/lambda
type: Collection
persistent identity: function recombination lambda
display id: function_recombination_lambda
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K112133
member: xis
member: BBa_K112247
member: xis domain
member: xis/int
member: BBa_I11021
member: xis/int/ih
member: BBa_K112327
member: BBa_K617000
member: BBa_K112142
member: BBa_K112246
member: Lambda att
member: BBa_I11020
member: BBa_K112248
member: BBa_K112137
member: BBa_K112141

xis
type: ComponentDefinition
persistent identity: BBa K112001
display id: BBa_K112001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Xis from bacteriophage lambda, assembly standard 21
creator: Bing Xia
created: 2008-10-19T11:00:00Z
modified: 2015-05-08T01:09:13Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: T4 holin gene without a start codon with a stop codon, in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable provenance: PCR bx3/bx2 on Bjh1301 (685 bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI) Product is pBca1256-K112001 ---------------------------------------- bx3 Forward biobricking of {<T4 Holin!} ccagtGAATTCatgAGATCTgcagcacctagaatatcattttcgccc bx2 Reverse biobricking of {<T4 Holin!} cgttaGGATCCttatttagcccttcctaatattctggccgcc
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K112001_sequence/1

BBa_I11021
type: ComponentDefinition
persistent identity: BBa I11021
display id: BBa_I11021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: excisionase from E. coli phage lambda (removes prophage from host genome)
creator: mschomp
created: 2004-09-02T11:00:00Z
modified: 2015-08-31T04:07:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Lambda excisionase coding region with AAV degradation tag and two stop codons
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cI lambda
sequence annotation: AAV
sequence annotation: Help:Barcodes
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_I11021_sequence/1

cI lambda
type: SequenceAnnotation
persistent identity: annotation1064105
display id: annotation1064105
version: 1
top level: BBa_I11021
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I11021/annotation1064105/range1064105/1

https://synbiohub.org/public/igem/BBa_I11021/annotation1064105/range1064105/1
type: Range
persistent identity: range1064105
display id: range1064105
version: 1
direction: direction/forward
top level: BBa_I11021
start: 1
end: 212
orientation: inline

AAV
type: SequenceAnnotation
persistent identity: annotation1064103
display id: annotation1064103
version: 1
top level: BBa_I11021
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_I11021/annotation1064103/range1064103/1

https://synbiohub.org/public/igem/BBa_I11021/annotation1064103/range1064103/1
type: Range
persistent identity: range1064103
display id: range1064103
version: 1
direction: direction/forward
top level: BBa_I11021
start: 213
end: 249
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214029
display id: annotation2214029
version: 1
top level: BBa_I11021
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_I11021/annotation2214029/range2214029/1

https://synbiohub.org/public/igem/BBa_I11021/annotation2214029/range2214029/1
type: Range
persistent identity: range2214029
display id: range2214029
version: 1
direction: direction/forward
top level: BBa_I11021
start: 256
end: 280
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1064104
display id: annotation1064104
version: 1
top level: BBa_I11021
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I11021/annotation1064104/range1064104/1

https://synbiohub.org/public/igem/BBa_I11021/annotation1064104/range1064104/1
type: Range
persistent identity: range1064104
display id: range1064104
version: 1
direction: direction/forward
top level: BBa_I11021
start: 250
end: 256
orientation: inline

Lambda att
type: ComponentDefinition
persistent identity: BBa I11022
display id: BBa_I11022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lambda attB, reverse complement
creator: mschomp
created: 2004-09-02T11:00:00Z
modified: 2015-08-31T04:07:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Lambda phage attB site, reverse complement (B'-O-B)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: lambda attB rev comp (B'-O-B)
sequence: https://synbiohub.org/public/igem/BBa_I11022_sequence/1

lambda attB rev comp (B'-O-B)
type: SequenceAnnotation
persistent identity: annotation1064122
display id: annotation1064122
version: 1
top level: Lambda att
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I11022/annotation1064122/range1064122/1

https://synbiohub.org/public/igem/BBa_I11022/annotation1064122/range1064122/1
type: Range
persistent identity: range1064122
display id: range1064122
version: 1
direction: direction/forward
top level: Lambda att
start: 1
end: 25
orientation: inline

BBa_K112137
type: ComponentDefinition
persistent identity: BBa K112137
display id: BBa_K112137
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Xis Int & TrfA combined expression cassette
creator: Madhvi Venkatesh
created: 2008-10-31T12:00:00Z
modified: 2015-05-08T01:09:14Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 3023
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Xis.Int under the control of a temperature sensitive promoter followed by a constitutively expressed TrfA gene.
mutable notes: This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: BBb Standard Description Page
mutable provenance: K112132+K112709+K112131
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K112999
component: BBa_K112709
component: BBa_K112126
component: BBa_K112234
component: BBa_K112131
sequence annotation: BBa_K112709
sequence annotation: BBa_K112131
sequence annotation: BBa_K112234
sequence annotation: BBa_K112999
sequence annotation: BBa_K112126
sequence: https://synbiohub.org/public/igem/BBa_K112137_sequence/1

BBa_K112999
type: Component
persistent identity: component1999128
display id: component1999128
version: 1
top level: BBa_K112137
access: public
definition: https://synbiohub.org/public/igem/BBa_K112999/1

BBa_K112999
type: Component
persistent identity: component1999137
display id: component1999137
version: 1
top level: BBa_K112137
access: public
definition: https://synbiohub.org/public/igem/BBa_K112999/1

BBa_K112709
type: Component
persistent identity: component1999136
display id: component1999136
version: 1
top level: BBa_K112137
access: public
definition: https://synbiohub.org/public/igem/BBa_K112709/1

BBa_K112126
type: Component
persistent identity: component1999127
display id: component1999127
version: 1
top level: BBa_K112137
access: public
definition: https://synbiohub.org/public/igem/BBa_K112126/1

BBa_K112234
type: Component
persistent identity: component1999134
display id: component1999134
version: 1
top level: BBa_K112137
access: public
definition: xis/int

BBa_K112131
type: Component
persistent identity: component1999138
display id: component1999138
version: 1
top level: BBa_K112137
access: public
definition: https://synbiohub.org/public/igem/BBa_K112131/1

BBa_K112999
type: Component
persistent identity: component1999135
display id: component1999135
version: 1
top level: BBa_K112137
access: public
definition: https://synbiohub.org/public/igem/BBa_K112999/1

BBa_K112709
type: SequenceAnnotation
persistent identity: annotation1999136
display id: annotation1999136
version: 1
top level: BBa_K112137
location: https://synbiohub.org/public/igem/BBa_K112137/annotation1999136/range1999136/1
component: BBa_K112709

https://synbiohub.org/public/igem/BBa_K112137/annotation1999136/range1999136/1
type: Range
persistent identity: range1999136
display id: range1999136
version: 1
direction: direction/reverse
top level: BBa_K112137
start: 2176
end: 2215
orientation: inline

BBa_K112131
type: SequenceAnnotation
persistent identity: annotation1999138
display id: annotation1999138
version: 1
top level: BBa_K112137
location: https://synbiohub.org/public/igem/BBa_K112137/annotation1999138/range1999138/1
component: BBa_K112131

https://synbiohub.org/public/igem/BBa_K112137/annotation1999138/range1999138/1
type: Range
persistent identity: range1999138
display id: range1999138
version: 1
direction: direction/reverse
top level: BBa_K112137
start: 2222
end: 2712
orientation: inline

BBa_K112234
type: SequenceAnnotation
persistent identity: annotation1999134
display id: annotation1999134
version: 1
top level: BBa_K112137
location: https://synbiohub.org/public/igem/BBa_K112137/annotation1999134/range1999134/1
component: BBa_K112234

https://synbiohub.org/public/igem/BBa_K112137/annotation1999134/range1999134/1
type: Range
persistent identity: range1999134
display id: range1999134
version: 1
direction: direction/reverse
top level: BBa_K112137
start: 884
end: 2169
orientation: inline

BBa_K112999
type: SequenceAnnotation
persistent identity: annotation1999128
display id: annotation1999128
version: 1
top level: BBa_K112137
location: https://synbiohub.org/public/igem/BBa_K112137/annotation1999128/range1999128/1
component: BBa_K112999

https://synbiohub.org/public/igem/BBa_K112137/annotation1999128/range1999128/1
type: Range
persistent identity: range1999128
display id: range1999128
version: 1
direction: direction/reverse
top level: BBa_K112137
start: 878
end: 883
orientation: inline

BBa_K112999
type: SequenceAnnotation
persistent identity: annotation1999135
display id: annotation1999135
version: 1
top level: BBa_K112137
location: https://synbiohub.org/public/igem/BBa_K112137/annotation1999135/range1999135/1
component: BBa_K112999

https://synbiohub.org/public/igem/BBa_K112137/annotation1999135/range1999135/1
type: Range
persistent identity: range1999135
display id: range1999135
version: 1
direction: direction/reverse
top level: BBa_K112137
start: 2170
end: 2175
orientation: inline

BBa_K112126
type: SequenceAnnotation
persistent identity: annotation1999127
display id: annotation1999127
version: 1
top level: BBa_K112137
location: https://synbiohub.org/public/igem/BBa_K112137/annotation1999127/range1999127/1
component: BBa_K112126

https://synbiohub.org/public/igem/BBa_K112137/annotation1999127/range1999127/1
type: Range
persistent identity: range1999127
display id: range1999127
version: 1
direction: direction/reverse
top level: BBa_K112137
start: 1
end: 877
orientation: inline

BBa_K112999
type: SequenceAnnotation
persistent identity: annotation1999137
display id: annotation1999137
version: 1
top level: BBa_K112137
location: https://synbiohub.org/public/igem/BBa_K112137/annotation1999137/range1999137/1
component: BBa_K112999

https://synbiohub.org/public/igem/BBa_K112137/annotation1999137/range1999137/1
type: Range
persistent identity: range1999137
display id: range1999137
version: 1
direction: direction/reverse
top level: BBa_K112137
start: 2216
end: 2221
orientation: inline

xis
type: ComponentDefinition
persistent identity: BBa K112204
display id: BBa_K112204
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {a~xis!} The bacteriophage lambda xis gene ready to have rbs attached and stop codon; assembly stand
creator: Molly Allen
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:14Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2048
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: ?
mutable provenance: pAH57 plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K112204_sequence/1

BBa_I11020
type: ComponentDefinition
persistent identity: BBa I11020
display id: BBa_I11020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: integrase from E. coli phage lambda
creator: bu
created: 2004-09-02T11:00:00Z
modified: 2015-08-31T04:07:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Wild type lambda integrase
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence annotation: Integrase
sequence: https://synbiohub.org/public/igem/BBa_I11020_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation1064102
display id: annotation1064102
version: 1
top level: BBa_I11020
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I11020/annotation1064102/range1064102/1

https://synbiohub.org/public/igem/BBa_I11020/annotation1064102/range1064102/1
type: Range
persistent identity: range1064102
display id: range1064102
version: 1
direction: direction/forward
top level: BBa_I11020
start: 1102
end: 1107
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1064100
display id: annotation1064100
version: 1
top level: BBa_I11020
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_I11020/annotation1064100/range1064100/1

https://synbiohub.org/public/igem/BBa_I11020/annotation1064100/range1064100/1
type: Range
persistent identity: range1064100
display id: range1064100
version: 1
direction: direction/forward
top level: BBa_I11020
start: 1069
end: 1101
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214027
display id: annotation2214027
version: 1
top level: BBa_I11020
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_I11020/annotation2214027/range2214027/1

https://synbiohub.org/public/igem/BBa_I11020/annotation2214027/range2214027/1
type: Range
persistent identity: range2214027
display id: range2214027
version: 1
direction: direction/forward
top level: BBa_I11020
start: 1108
end: 1132
orientation: inline

Integrase
type: SequenceAnnotation
persistent identity: annotation1064101
display id: annotation1064101
version: 1
top level: BBa_I11020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I11020/annotation1064101/range1064101/1

https://synbiohub.org/public/igem/BBa_I11020/annotation1064101/range1064101/1
type: Range
persistent identity: range1064101
display id: range1064101
version: 1
direction: direction/forward
top level: BBa_I11020
start: 1
end: 1068
orientation: inline

BBa_K112142
type: ComponentDefinition
persistent identity: BBa K112142
display id: BBa_K112142
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: attR2 recombination site-reverse orientation
creator: Madhvi Venkatesh
created: 2008-10-30T12:00:00Z
modified: 2015-05-08T01:09:14Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 3023
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: attR2 recombination site in reverse orientation
mutable notes: This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: BBb Standard Description Page
mutable provenance: Anderson lab assembly vectors
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K112142_sequence/1

xis domain
type: ComponentDefinition
persistent identity: BBa K112203
display id: BBa_K112203
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {< xis >} The bacteriophage lambda xis gene without start or stop codons; assembly standard 21
creator: Molly Allen
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:14Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2048
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: ?
mutable provenance: pAH57 plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K112203_sequence/1

xis
type: ComponentDefinition
persistent identity: BBa K112205
display id: BBa_K112205
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {rbs.xis!} The xis gene with native rbs site and stop codon; assembly standard 21
creator: Molly Allen
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:14Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2048
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: ?
mutable provenance: pAH57 plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K112205_sequence/1

BBa_K112246
type: ComponentDefinition
persistent identity: BBa K112246
display id: BBa_K112246
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {pAH57 promoter}{rbs.xis.int!}{rbs.ihfA!}{rbs.ihfB!} inserted into K112995 plasmid
creator: Molly Allen
created: 2008-10-28T12:00:00Z
modified: 2015-06-15T01:10:45Z
discontinued: true
dominant: false
experience: experience/Works
group u list: _224_
m user id: 4206
owner id: 2048
owning group id: 9
sample status: It's complicated
status: status/Deleted
bookmark: false
mutable description: This part is inserted into the K112995 plasmid between ccdB and attR2. This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: ?
mutable provenance: pAH57 plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence annotation: rbs.ihfB!
sequence annotation: ccdB
sequence annotation: rbs.ihfA!
sequence annotation: pAH57 promoter
sequence annotation: attR1
sequence annotation: rbs.xis.int!
sequence annotation: attR2
sequence: https://synbiohub.org/public/igem/BBa_K112246_sequence/1

rbs.ihfB!
type: SequenceAnnotation
persistent identity: annotation1993946
display id: annotation1993946
version: 1
top level: BBa_K112246
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K112246/annotation1993946/range1993946/1

https://synbiohub.org/public/igem/BBa_K112246/annotation1993946/range1993946/1
type: Range
persistent identity: range1993946
display id: range1993946
version: 1
direction: direction/forward
top level: BBa_K112246
start: 3393
end: 3673
orientation: inline

ccdB
type: SequenceAnnotation
persistent identity: annotation1993931
display id: annotation1993931
version: 1
top level: BBa_K112246
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K112246/annotation1993931/range1993931/1

https://synbiohub.org/public/igem/BBa_K112246/annotation1993931/range1993931/1
type: Range
persistent identity: range1993931
display id: range1993931
version: 1
direction: direction/forward
top level: BBa_K112246
start: 640
end: 849
orientation: inline

rbs.ihfA!
type: SequenceAnnotation
persistent identity: annotation1993934
display id: annotation1993934
version: 1
top level: BBa_K112246
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K112246/annotation1993934/range1993934/1

https://synbiohub.org/public/igem/BBa_K112246/annotation1993934/range1993934/1
type: Range
persistent identity: range1993934
display id: range1993934
version: 1
direction: direction/forward
top level: BBa_K112246
start: 3068
end: 3386
orientation: inline

pAH57 promoter
type: SequenceAnnotation
persistent identity: annotation1993930
display id: annotation1993930
version: 1
top level: BBa_K112246
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K112246/annotation1993930/range1993930/1

https://synbiohub.org/public/igem/BBa_K112246/annotation1993930/range1993930/1
type: Range
persistent identity: range1993930
display id: range1993930
version: 1
direction: direction/forward
top level: BBa_K112246
start: 893
end: 1769
orientation: inline

attR1
type: SequenceAnnotation
persistent identity: annotation1993932
display id: annotation1993932
version: 1
top level: BBa_K112246
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K112246/annotation1993932/range1993932/1

https://synbiohub.org/public/igem/BBa_K112246/annotation1993932/range1993932/1
type: Range
persistent identity: range1993932
display id: range1993932
version: 1
direction: direction/forward
top level: BBa_K112246
start: 16
end: 140
orientation: inline

rbs.xis.int!
type: SequenceAnnotation
persistent identity: annotation1993933
display id: annotation1993933
version: 1
top level: BBa_K112246
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K112246/annotation1993933/range1993933/1

https://synbiohub.org/public/igem/BBa_K112246/annotation1993933/range1993933/1
type: Range
persistent identity: range1993933
display id: range1993933
version: 1
direction: direction/forward
top level: BBa_K112246
start: 1776
end: 3061
orientation: inline

attR2
type: SequenceAnnotation
persistent identity: annotation1993947
display id: annotation1993947
version: 1
top level: BBa_K112246
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K112246/annotation1993947/range1993947/1

https://synbiohub.org/public/igem/BBa_K112246/annotation1993947/range1993947/1
type: Range
persistent identity: range1993947
display id: range1993947
version: 1
direction: direction/forward
top level: BBa_K112246
start: 3686
end: 3810
orientation: inline

BBa_K112141
type: ComponentDefinition
persistent identity: BBa K112141
display id: BBa_K112141
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: attR2 recombination site
creator: Madhvi Venkatesh
created: 2008-10-31T12:00:00Z
modified: 2015-05-08T01:09:14Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 3023
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: attR2 recombination site
mutable notes: This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: BBb Standard Description Page
mutable provenance: Anderson lab assembly vectors
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K112141_sequence/1

BBa_K617000
type: ComponentDefinition
persistent identity: BBa K617000
display id: BBa_K617000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Biobrick Compatible Lambda Chromosomal Insertion Plasmid
creator: Will Jones
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _789_
m user id: 0
owner id: 4301
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: .
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence annotation: Lambda attP P'OP
sequence annotation: Biobrick Site Prefix -> Suffix
sequence annotation: rgnB terminator
sequence annotation: R6K Origin of Replication
sequence annotation: KanR
sequence annotation: tL3 terminator
sequence: https://synbiohub.org/public/igem/BBa_K617000_sequence/1

Lambda attP P'OP
type: SequenceAnnotation
persistent identity: annotation2145554
display id: annotation2145554
version: 1
top level: BBa_K617000
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K617000/annotation2145554/range2145554/1

https://synbiohub.org/public/igem/BBa_K617000/annotation2145554/range2145554/1
type: Range
persistent identity: range2145554
display id: range2145554
version: 1
direction: direction/forward
top level: BBa_K617000
start: 276
end: 671
orientation: inline

Biobrick Site Prefix -> Suffix
type: SequenceAnnotation
persistent identity: annotation2145558
display id: annotation2145558
version: 1
top level: BBa_K617000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K617000/annotation2145558/range2145558/1

https://synbiohub.org/public/igem/BBa_K617000/annotation2145558/range2145558/1
type: Range
persistent identity: range2145558
display id: range2145558
version: 1
direction: direction/forward
top level: BBa_K617000
start: 2398
end: 2440
orientation: inline

rgnB terminator
type: SequenceAnnotation
persistent identity: annotation2145557
display id: annotation2145557
version: 1
top level: BBa_K617000
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K617000/annotation2145557/range2145557/1

https://synbiohub.org/public/igem/BBa_K617000/annotation2145557/range2145557/1
type: Range
persistent identity: range2145557
display id: range2145557
version: 1
direction: direction/forward
top level: BBa_K617000
start: 2116
end: 2375
orientation: inline

R6K Origin of Replication
type: SequenceAnnotation
persistent identity: annotation2145555
display id: annotation2145555
version: 1
top level: BBa_K617000
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K617000/annotation2145555/range2145555/1

https://synbiohub.org/public/igem/BBa_K617000/annotation2145555/range2145555/1
type: Range
persistent identity: range2145555
display id: range2145555
version: 1
direction: direction/forward
top level: BBa_K617000
start: 679
end: 1070
orientation: inline

KanR
type: SequenceAnnotation
persistent identity: annotation2145556
display id: annotation2145556
version: 1
top level: BBa_K617000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K617000/annotation2145556/range2145556/1

https://synbiohub.org/public/igem/BBa_K617000/annotation2145556/range2145556/1
type: Range
persistent identity: range2145556
display id: range2145556
version: 1
direction: direction/reverse
top level: BBa_K617000
start: 1157
end: 1951
orientation: inline

tL3 terminator
type: SequenceAnnotation
persistent identity: annotation2145553
display id: annotation2145553
version: 1
top level: BBa_K617000
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K617000/annotation2145553/range2145553/1

https://synbiohub.org/public/igem/BBa_K617000/annotation2145553/range2145553/1
type: Range
persistent identity: range2145553
display id: range2145553
version: 1
direction: direction/forward
top level: BBa_K617000
start: 1
end: 270
orientation: inline

xis domain
type: ComponentDefinition
persistent identity: BBa K112202
display id: BBa_K112202
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {xis>} The lambda xis gene with no stop codon; assembly standard 21
creator: Molly Allen
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:14Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2048
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: ?
mutable provenance: pAH57 plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K112202_sequence/1

BBa_K112248
type: ComponentDefinition
persistent identity: BBa K112248
display id: BBa_K112248
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {pAH57 promoter}{rbs.xis.int!}{rbs.ihfA!}{rbs.ihfB!} inserted into K112950 plasmid
creator: Molly Allen
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:09:15Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _224_
m user id: 0
owner id: 2048
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is inserted into the K112950 plasmid just before attL1. This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: ?
mutable provenance: pAH57 plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence annotation: rbs.ihfA!
sequence annotation: rbs.ihfB!
sequence annotation: pAH57 promoter
sequence annotation: mRFP
sequence annotation: attL2
sequence annotation: attL1
sequence annotation: rbs.xis.int!
sequence: https://synbiohub.org/public/igem/BBa_K112248_sequence/1

rbs.ihfA!
type: SequenceAnnotation
persistent identity: annotation1993973
display id: annotation1993973
version: 1
top level: BBa_K112248
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K112248/annotation1993973/range1993973/1

https://synbiohub.org/public/igem/BBa_K112248/annotation1993973/range1993973/1
type: Range
persistent identity: range1993973
display id: range1993973
version: 1
direction: direction/forward
top level: BBa_K112248
start: 2250
end: 2568
orientation: inline

rbs.ihfB!
type: SequenceAnnotation
persistent identity: annotation1993974
display id: annotation1993974
version: 1
top level: BBa_K112248
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K112248/annotation1993974/range1993974/1

https://synbiohub.org/public/igem/BBa_K112248/annotation1993974/range1993974/1
type: Range
persistent identity: range1993974
display id: range1993974
version: 1
direction: direction/forward
top level: BBa_K112248
start: 2575
end: 2880
orientation: inline

pAH57 promoter
type: SequenceAnnotation
persistent identity: annotation1993971
display id: annotation1993971
version: 1
top level: BBa_K112248
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K112248/annotation1993971/range1993971/1

https://synbiohub.org/public/igem/BBa_K112248/annotation1993971/range1993971/1
type: Range
persistent identity: range1993971
display id: range1993971
version: 1
direction: direction/forward
top level: BBa_K112248
start: 75
end: 951
orientation: inline

mRFP
type: SequenceAnnotation
persistent identity: annotation1993976
display id: annotation1993976
version: 1
top level: BBa_K112248
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K112248/annotation1993976/range1993976/1

https://synbiohub.org/public/igem/BBa_K112248/annotation1993976/range1993976/1
type: Range
persistent identity: range1993976
display id: range1993976
version: 1
direction: direction/forward
top level: BBa_K112248
start: 3160
end: 3837
orientation: inline

attL2
type: SequenceAnnotation
persistent identity: annotation1993977
display id: annotation1993977
version: 1
top level: BBa_K112248
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K112248/annotation1993977/range1993977/1

https://synbiohub.org/public/igem/BBa_K112248/annotation1993977/range1993977/1
type: Range
persistent identity: range1993977
display id: range1993977
version: 1
direction: direction/forward
top level: BBa_K112248
start: 4081
end: 4180
orientation: inline

attL1
type: SequenceAnnotation
persistent identity: annotation1993975
display id: annotation1993975
version: 1
top level: BBa_K112248
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K112248/annotation1993975/range1993975/1

https://synbiohub.org/public/igem/BBa_K112248/annotation1993975/range1993975/1
type: Range
persistent identity: range1993975
display id: range1993975
version: 1
direction: direction/forward
top level: BBa_K112248
start: 2897
end: 2996
orientation: inline

rbs.xis.int!
type: SequenceAnnotation
persistent identity: annotation1993972
display id: annotation1993972
version: 1
top level: BBa_K112248
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K112248/annotation1993972/range1993972/1

https://synbiohub.org/public/igem/BBa_K112248/annotation1993972/range1993972/1
type: Range
persistent identity: range1993972
display id: range1993972
version: 1
direction: direction/forward
top level: BBa_K112248
start: 958
end: 2243
orientation: inline

BBa_K112327
type: ComponentDefinition
persistent identity: BBa K112327
display id: BBa_K112327
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {rbs_pelB>} {< xis>}
creator: Aron Lau
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:09:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2999
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: {rbs_pelB>} {< xis>} ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: Digest (BgIII methylated) K112230 AK, (BamHI methylated) K112203 KC (BamHI/BgIII/XHoI) Product is pBjh1601AC-K112327
mutable provenance: K112230 AK and K112203 KC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
component: BBa_K112999
component: BBa_K112230
component: BBa_K112203
sequence annotation: BBa_K112203
sequence annotation: BBa_K112230
sequence annotation: BBa_K112999
sequence: https://synbiohub.org/public/igem/BBa_K112327_sequence/1

BBa_K112999
type: Component
persistent identity: component1994551
display id: component1994551
version: 1
top level: BBa_K112327
access: public
definition: https://synbiohub.org/public/igem/BBa_K112999/1

BBa_K112230
type: Component
persistent identity: component1994550
display id: component1994550
version: 1
top level: BBa_K112327
access: public
definition: https://synbiohub.org/public/igem/BBa_K112230/1

BBa_K112203
type: Component
persistent identity: component1994552
display id: component1994552
version: 1
top level: BBa_K112327
access: public
definition: xis domain

BBa_K112203
type: SequenceAnnotation
persistent identity: annotation1994552
display id: annotation1994552
version: 1
top level: BBa_K112327
location: https://synbiohub.org/public/igem/BBa_K112327/annotation1994552/range1994552/1
component: BBa_K112203

https://synbiohub.org/public/igem/BBa_K112327/annotation1994552/range1994552/1
type: Range
persistent identity: range1994552
display id: range1994552
version: 1
direction: direction/reverse
top level: BBa_K112327
start: 92
end: 304
orientation: inline

BBa_K112230
type: SequenceAnnotation
persistent identity: annotation1994550
display id: annotation1994550
version: 1
top level: BBa_K112327
location: https://synbiohub.org/public/igem/BBa_K112327/annotation1994550/range1994550/1
component: BBa_K112230

https://synbiohub.org/public/igem/BBa_K112327/annotation1994550/range1994550/1
type: Range
persistent identity: range1994550
display id: range1994550
version: 1
direction: direction/reverse
top level: BBa_K112327
start: 1
end: 85
orientation: inline

BBa_K112999
type: SequenceAnnotation
persistent identity: annotation1994551
display id: annotation1994551
version: 1
top level: BBa_K112327
location: https://synbiohub.org/public/igem/BBa_K112327/annotation1994551/range1994551/1
component: BBa_K112999

https://synbiohub.org/public/igem/BBa_K112327/annotation1994551/range1994551/1
type: Range
persistent identity: range1994551
display id: range1994551
version: 1
direction: direction/reverse
top level: BBa_K112327
start: 86
end: 91
orientation: inline

xis/int/ih
type: ComponentDefinition
persistent identity: BBa K112237
display id: BBa_K112237
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {rbs.xis.int}{rbs.ihfA}{rbs.ihfB}
creator: Bing Xia
created: 2008-10-20T11:00:00Z
modified: 2015-06-15T01:17:04Z
discontinued: true
dominant: false
experience: experience/None
group u list: _224_
m user id: 4206
owner id: 2998
owning group id: 9
sample status: It's complicated
status: status/Deleted
bookmark: false
mutable description: ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K112999
component: BBa_K112218
component: BBa_K112224
component: BBa_K112901
sequence annotation: BBa_K112999
sequence annotation: BBa_K112224
sequence annotation: BBa_K112901
sequence annotation: BBa_K112218
sequence: https://synbiohub.org/public/igem/BBa_K112237_sequence/1

BBa_K112999
type: Component
persistent identity: component1983841
display id: component1983841
version: 1
top level: xis/int/ih
access: public
definition: https://synbiohub.org/public/igem/BBa_K112999/1

BBa_K112218
type: Component
persistent identity: component1983840
display id: component1983840
version: 1
top level: xis/int/ih
access: public
definition: https://synbiohub.org/public/igem/BBa_K112218/1

BBa_K112999
type: Component
persistent identity: component1983843
display id: component1983843
version: 1
top level: xis/int/ih
access: public
definition: https://synbiohub.org/public/igem/BBa_K112999/1

BBa_K112224
type: Component
persistent identity: component1983842
display id: component1983842
version: 1
top level: xis/int/ih
access: public
definition: https://synbiohub.org/public/igem/BBa_K112224/1

BBa_K112901
type: Component
persistent identity: component1983844
display id: component1983844
version: 1
top level: xis/int/ih
access: public
definition: https://synbiohub.org/public/igem/BBa_K112901/1

BBa_K112999
type: SequenceAnnotation
persistent identity: annotation1983841
display id: annotation1983841
version: 1
top level: xis/int/ih
location: https://synbiohub.org/public/igem/BBa_K112237/annotation1983841/range1983841/1
component: BBa_K112999

https://synbiohub.org/public/igem/BBa_K112237/annotation1983841/range1983841/1
type: Range
persistent identity: range1983841
display id: range1983841
version: 1
direction: direction/reverse
top level: xis/int/ih
start: 328
end: 333
orientation: inline

BBa_K112999
type: SequenceAnnotation
persistent identity: annotation1983843
display id: annotation1983843
version: 1
top level: xis/int/ih
location: https://synbiohub.org/public/igem/BBa_K112237/annotation1983843/range1983843/1
component: BBa_K112999

https://synbiohub.org/public/igem/BBa_K112237/annotation1983843/range1983843/1
type: Range
persistent identity: range1983843
display id: range1983843
version: 1
direction: direction/reverse
top level: xis/int/ih
start: 656
end: 661
orientation: inline

BBa_K112224
type: SequenceAnnotation
persistent identity: annotation1983842
display id: annotation1983842
version: 1
top level: xis/int/ih
location: https://synbiohub.org/public/igem/BBa_K112237/annotation1983842/range1983842/1
component: BBa_K112224

https://synbiohub.org/public/igem/BBa_K112237/annotation1983842/range1983842/1
type: Range
persistent identity: range1983842
display id: range1983842
version: 1
direction: direction/reverse
top level: xis/int/ih
start: 342
end: 647
orientation: inline

BBa_K112901
type: SequenceAnnotation
persistent identity: annotation1983844
display id: annotation1983844
version: 1
top level: xis/int/ih
location: https://synbiohub.org/public/igem/BBa_K112237/annotation1983844/range1983844/1
component: BBa_K112901

https://synbiohub.org/public/igem/BBa_K112237/annotation1983844/range1983844/1
type: Range
persistent identity: range1983844
display id: range1983844
version: 1
direction: direction/reverse
top level: xis/int/ih
start: 670
end: 1101
orientation: inline

BBa_K112218
type: SequenceAnnotation
persistent identity: annotation1983840
display id: annotation1983840
version: 1
top level: xis/int/ih
location: https://synbiohub.org/public/igem/BBa_K112237/annotation1983840/range1983840/1
component: BBa_K112218

https://synbiohub.org/public/igem/BBa_K112237/annotation1983840/range1983840/1
type: Range
persistent identity: range1983840
display id: range1983840
version: 1
direction: direction/reverse
top level: xis/int/ih
start: 1
end: 319
orientation: inline

xis/int
type: ComponentDefinition
persistent identity: BBa K112132
display id: BBa_K112132
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Xis Int controlled by temperature sensitive
creator: Madhvi Venkatesh
created: 2008-10-30T12:00:00Z
modified: 2015-05-08T01:09:14Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 3023
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Xis.int (with native rbs) under the comtrol of a temperature sensitive promoter. terminator follows the CDS.
mutable notes: This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: BBb Standard Description Page
mutable provenance: K112126+K112234+K112709
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K112999
component: BBa_K112126
component: BBa_K112234
sequence annotation: BBa_K112126
sequence annotation: BBa_K112234
sequence annotation: BBa_K112999
sequence: https://synbiohub.org/public/igem/BBa_K112132_sequence/1

BBa_K112999
type: Component
persistent identity: component1998983
display id: component1998983
version: 1
top level: xis/int
access: public
definition: https://synbiohub.org/public/igem/BBa_K112999/1

BBa_K112126
type: Component
persistent identity: component1998982
display id: component1998982
version: 1
top level: xis/int
access: public
definition: https://synbiohub.org/public/igem/BBa_K112126/1

BBa_K112234
type: Component
persistent identity: component1998989
display id: component1998989
version: 1
top level: xis/int
access: public
definition: xis/int

BBa_K112126
type: SequenceAnnotation
persistent identity: annotation1998982
display id: annotation1998982
version: 1
top level: xis/int
location: https://synbiohub.org/public/igem/BBa_K112132/annotation1998982/range1998982/1
component: BBa_K112126

https://synbiohub.org/public/igem/BBa_K112132/annotation1998982/range1998982/1
type: Range
persistent identity: range1998982
display id: range1998982
version: 1
direction: direction/reverse
top level: xis/int
start: 1
end: 877
orientation: inline

BBa_K112234
type: SequenceAnnotation
persistent identity: annotation1998989
display id: annotation1998989
version: 1
top level: xis/int
location: https://synbiohub.org/public/igem/BBa_K112132/annotation1998989/range1998989/1
component: BBa_K112234

https://synbiohub.org/public/igem/BBa_K112132/annotation1998989/range1998989/1
type: Range
persistent identity: range1998989
display id: range1998989
version: 1
direction: direction/reverse
top level: xis/int
start: 884
end: 2169
orientation: inline

BBa_K112999
type: SequenceAnnotation
persistent identity: annotation1998983
display id: annotation1998983
version: 1
top level: xis/int
location: https://synbiohub.org/public/igem/BBa_K112132/annotation1998983/range1998983/1
component: BBa_K112999

https://synbiohub.org/public/igem/BBa_K112132/annotation1998983/range1998983/1
type: Range
persistent identity: range1998983
display id: range1998983
version: 1
direction: direction/reverse
top level: xis/int
start: 878
end: 883
orientation: inline

xis
type: ComponentDefinition
persistent identity: BBa K112200
display id: BBa_K112200
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {xis!} from bacteriophage lambda; assembly standard 21
creator: Molly Allen
created: 2008-10-19T11:00:00Z
modified: 2015-05-08T01:09:14Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2048
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: ?
mutable provenance: pAH57 plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K112200_sequence/1

BBa_K112247
type: ComponentDefinition
persistent identity: BBa K112247
display id: BBa_K112247
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {pAH57 promoter}{rbs.xis.int!} inserted into K112950 plasmid
creator: Molly Allen
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:09:15Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _224_
m user id: 0
owner id: 2048
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is inserted just before the attL1 site in this plasmid. This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: ?
mutable provenance: pAH57 plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence annotation: attL2
sequence annotation: rbs.xis.int!
sequence annotation: mRFP
sequence annotation: pAH57 promoter
sequence annotation: attL1
sequence: https://synbiohub.org/public/igem/BBa_K112247_sequence/1

attL2
type: SequenceAnnotation
persistent identity: annotation1993970
display id: annotation1993970
version: 1
top level: BBa_K112247
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K112247/annotation1993970/range1993970/1

https://synbiohub.org/public/igem/BBa_K112247/annotation1993970/range1993970/1
type: Range
persistent identity: range1993970
display id: range1993970
version: 1
direction: direction/forward
top level: BBa_K112247
start: 3444
end: 3543
orientation: inline

rbs.xis.int!
type: SequenceAnnotation
persistent identity: annotation1993967
display id: annotation1993967
version: 1
top level: BBa_K112247
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K112247/annotation1993967/range1993967/1

https://synbiohub.org/public/igem/BBa_K112247/annotation1993967/range1993967/1
type: Range
persistent identity: range1993967
display id: range1993967
version: 1
direction: direction/forward
top level: BBa_K112247
start: 958
end: 2243
orientation: inline

mRFP
type: SequenceAnnotation
persistent identity: annotation1993969
display id: annotation1993969
version: 1
top level: BBa_K112247
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K112247/annotation1993969/range1993969/1

https://synbiohub.org/public/igem/BBa_K112247/annotation1993969/range1993969/1
type: Range
persistent identity: range1993969
display id: range1993969
version: 1
direction: direction/forward
top level: BBa_K112247
start: 2523
end: 3200
orientation: inline

pAH57 promoter
type: SequenceAnnotation
persistent identity: annotation1993966
display id: annotation1993966
version: 1
top level: BBa_K112247
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K112247/annotation1993966/range1993966/1

https://synbiohub.org/public/igem/BBa_K112247/annotation1993966/range1993966/1
type: Range
persistent identity: range1993966
display id: range1993966
version: 1
direction: direction/forward
top level: BBa_K112247
start: 75
end: 951
orientation: inline

attL1
type: SequenceAnnotation
persistent identity: annotation1993968
display id: annotation1993968
version: 1
top level: BBa_K112247
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K112247/annotation1993968/range1993968/1

https://synbiohub.org/public/igem/BBa_K112247/annotation1993968/range1993968/1
type: Range
persistent identity: range1993968
display id: range1993968
version: 1
direction: direction/forward
top level: BBa_K112247
start: 2260
end: 2359
orientation: inline

Lambda att
type: ComponentDefinition
persistent identity: BBa I11023
display id: BBa_I11023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lambda attP
creator: bu
created: 2004-09-02T11:00:00Z
modified: 2015-08-31T04:07:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Lambda phage attP binding site P-O-P'
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: lambda phage attp site
sequence: https://synbiohub.org/public/igem/BBa_I11023_sequence/1

lambda phage attp site
type: SequenceAnnotation
persistent identity: annotation1064123
display id: annotation1064123
version: 1
top level: Lambda att
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I11023/annotation1064123/range1064123/1

https://synbiohub.org/public/igem/BBa_I11023/annotation1064123/range1064123/1
type: Range
persistent identity: range1064123
display id: range1064123
version: 1
direction: direction/forward
top level: Lambda att
start: 1
end: 232
orientation: inline

xis/int
type: ComponentDefinition
persistent identity: BBa K112234
display id: BBa_K112234
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {rbs.xis.int!} The bacteriophage lambda xis/integrase gene with native rbs and stop codon; assembly
creator: Molly Allen
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2048
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: The xis and int genes naturally overlap, so instead of trying to isolate both the xis and int and put them together, I decided to make them one entire basic part.
mutable provenance: pAH57 plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: int
sequence annotation: rbs
sequence annotation: start
sequence annotation: stop
sequence annotation: xis
sequence: https://synbiohub.org/public/igem/BBa_K112234_sequence/1

int
type: SequenceAnnotation
persistent identity: annotation1983027
display id: annotation1983027
version: 1
top level: xis/int
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K112234/annotation1983027/range1983027/1

https://synbiohub.org/public/igem/BBa_K112234/annotation1983027/range1983027/1
type: Range
persistent identity: range1983027
display id: range1983027
version: 1
direction: direction/forward
top level: xis/int
start: 216
end: 1286
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation1983025
display id: annotation1983025
version: 1
top level: xis/int
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K112234/annotation1983025/range1983025/1

https://synbiohub.org/public/igem/BBa_K112234/annotation1983025/range1983025/1
type: Range
persistent identity: range1983025
display id: range1983025
version: 1
direction: direction/forward
top level: xis/int
start: 1
end: 16
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1983028
display id: annotation1983028
version: 1
top level: xis/int
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K112234/annotation1983028/range1983028/1

https://synbiohub.org/public/igem/BBa_K112234/annotation1983028/range1983028/1
type: Range
persistent identity: range1983028
display id: range1983028
version: 1
direction: direction/forward
top level: xis/int
start: 20
end: 22
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1983029
display id: annotation1983029
version: 1
top level: xis/int
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K112234/annotation1983029/range1983029/1

https://synbiohub.org/public/igem/BBa_K112234/annotation1983029/range1983029/1
type: Range
persistent identity: range1983029
display id: range1983029
version: 1
direction: direction/forward
top level: xis/int
start: 1284
end: 1286
orientation: inline

xis
type: SequenceAnnotation
persistent identity: annotation1983026
display id: annotation1983026
version: 1
top level: xis/int
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K112234/annotation1983026/range1983026/1

https://synbiohub.org/public/igem/BBa_K112234/annotation1983026/range1983026/1
type: Range
persistent identity: range1983026
display id: range1983026
version: 1
direction: direction/forward
top level: xis/int
start: 20
end: 237
orientation: inline

BBa_K112133
type: ComponentDefinition
persistent identity: BBa K112133
display id: BBa_K112133
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Xis Int temperature sensitive expression cassette
creator: Madhvi Venkatesh
created: 2008-10-30T12:00:00Z
modified: 2015-05-08T01:09:14Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 3023
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Xis int (with native rbs) under the control of a temperature sensitive promoter. Terminator follows CDS.
mutable notes: This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: BBb Standard Description Page
mutable provenance: K112132+K112709
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K112709
component: BBa_K112999
component: BBa_K112234
component: BBa_K112126
sequence annotation: BBa_K112999
sequence annotation: BBa_K112709
sequence annotation: BBa_K112234
sequence annotation: BBa_K112126
sequence: https://synbiohub.org/public/igem/BBa_K112133_sequence/1

BBa_K112709
type: Component
persistent identity: component1998999
display id: component1998999
version: 1
top level: BBa_K112133
access: public
definition: https://synbiohub.org/public/igem/BBa_K112709/1

BBa_K112999
type: Component
persistent identity: component1998991
display id: component1998991
version: 1
top level: BBa_K112133
access: public
definition: https://synbiohub.org/public/igem/BBa_K112999/1

BBa_K112234
type: Component
persistent identity: component1998997
display id: component1998997
version: 1
top level: BBa_K112133
access: public
definition: xis/int

BBa_K112126
type: Component
persistent identity: component1998990
display id: component1998990
version: 1
top level: BBa_K112133
access: public
definition: https://synbiohub.org/public/igem/BBa_K112126/1

BBa_K112999
type: Component
persistent identity: component1998998
display id: component1998998
version: 1
top level: BBa_K112133
access: public
definition: https://synbiohub.org/public/igem/BBa_K112999/1

BBa_K112999
type: SequenceAnnotation
persistent identity: annotation1998991
display id: annotation1998991
version: 1
top level: BBa_K112133
location: https://synbiohub.org/public/igem/BBa_K112133/annotation1998991/range1998991/1
component: BBa_K112999

https://synbiohub.org/public/igem/BBa_K112133/annotation1998991/range1998991/1
type: Range
persistent identity: range1998991
display id: range1998991
version: 1
direction: direction/reverse
top level: BBa_K112133
start: 878
end: 883
orientation: inline

BBa_K112999
type: SequenceAnnotation
persistent identity: annotation1998998
display id: annotation1998998
version: 1
top level: BBa_K112133
location: https://synbiohub.org/public/igem/BBa_K112133/annotation1998998/range1998998/1
component: BBa_K112999

https://synbiohub.org/public/igem/BBa_K112133/annotation1998998/range1998998/1
type: Range
persistent identity: range1998998
display id: range1998998
version: 1
direction: direction/reverse
top level: BBa_K112133
start: 2170
end: 2175
orientation: inline

BBa_K112709
type: SequenceAnnotation
persistent identity: annotation1998999
display id: annotation1998999
version: 1
top level: BBa_K112133
location: https://synbiohub.org/public/igem/BBa_K112133/annotation1998999/range1998999/1
component: BBa_K112709

https://synbiohub.org/public/igem/BBa_K112133/annotation1998999/range1998999/1
type: Range
persistent identity: range1998999
display id: range1998999
version: 1
direction: direction/reverse
top level: BBa_K112133
start: 2176
end: 2215
orientation: inline

BBa_K112234
type: SequenceAnnotation
persistent identity: annotation1998997
display id: annotation1998997
version: 1
top level: BBa_K112133
location: https://synbiohub.org/public/igem/BBa_K112133/annotation1998997/range1998997/1
component: BBa_K112234

https://synbiohub.org/public/igem/BBa_K112133/annotation1998997/range1998997/1
type: Range
persistent identity: range1998997
display id: range1998997
version: 1
direction: direction/reverse
top level: BBa_K112133
start: 884
end: 2169
orientation: inline

BBa_K112126
type: SequenceAnnotation
persistent identity: annotation1998990
display id: annotation1998990
version: 1
top level: BBa_K112133
location: https://synbiohub.org/public/igem/BBa_K112133/annotation1998990/range1998990/1
component: BBa_K112126

https://synbiohub.org/public/igem/BBa_K112133/annotation1998990/range1998990/1
type: Range
persistent identity: range1998990
display id: range1998990
version: 1
direction: direction/reverse
top level: BBa_K112133
start: 1
end: 877
orientation: inline