//function/recombination/cre
type: Collection
persistent identity: function recombination cre
display id: function_recombination_cre
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K1926011
member: lox71
member: BBa_K1680017
member: BBa_I718008
member: BBa_K1680020
member: BBa_K886001
member: BBa_I716213
member: BBa_K416002
member: BBa_K886003
member: BBa_K1680022
member: BBa_K1680005
member: lox66
member: BBa_K1680018
member: BBa_K1680019
member: BBa_I716208
member: RBS.cre
member: BBa_K112100
member: BBa_K886000
member: BBa_I716212
member: BBa_K132027
member: BBa_I718007
member: BBa_K112122
member: BBa_K1680023
member: BBa_K112101
member: BBa_K1680007
member: BBa_K132028
member: BBa_K1680024
member: lox
member: BBa_K112115
member: cre
member: BBa_K082011
member: BBa_K1680008
member: BBa_K1680021
member: Cre
member: BBa_K886002
member: BBa_K1680025

BBa_I718008
type: ComponentDefinition
persistent identity: BBa I718008
display id: BBa_I718008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: araC-pBad-rbs-cre
creator: David Bikard
created: 2007-10-25T11:00:00Z
modified: 2015-08-31T04:07:52Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _141_
m user id: 0
owner id: 1481
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: none
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J61047
component: BBa_I0500
component: BBa_B0030
sequence annotation: BBa_B0030
sequence annotation: BBa_I0500
sequence annotation: BBa_J61047
sequence: https://synbiohub.org/public/igem/BBa_I718008_sequence/1

BBa_J61047
type: Component
persistent identity: component1956083
display id: component1956083
version: 1
top level: BBa_I718008
access: public
definition: Cre

BBa_I0500
type: Component
persistent identity: component1956079
display id: component1956079
version: 1
top level: BBa_I718008
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0030
type: Component
persistent identity: component1956081
display id: component1956081
version: 1
top level: BBa_I718008
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1956081
display id: annotation1956081
version: 1
top level: BBa_I718008
location: https://synbiohub.org/public/igem/BBa_I718008/annotation1956081/range1956081/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_I718008/annotation1956081/range1956081/1
type: Range
persistent identity: range1956081
display id: range1956081
version: 1
direction: direction/forward
top level: BBa_I718008
start: 1219
end: 1233
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation1956079
display id: annotation1956079
version: 1
top level: BBa_I718008
location: https://synbiohub.org/public/igem/BBa_I718008/annotation1956079/range1956079/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_I718008/annotation1956079/range1956079/1
type: Range
persistent identity: range1956079
display id: range1956079
version: 1
direction: direction/reverse
top level: BBa_I718008
start: 1
end: 1210
orientation: inline

BBa_J61047
type: SequenceAnnotation
persistent identity: annotation1956083
display id: annotation1956083
version: 1
top level: BBa_I718008
location: https://synbiohub.org/public/igem/BBa_I718008/annotation1956083/range1956083/1
component: BBa_J61047

https://synbiohub.org/public/igem/BBa_I718008/annotation1956083/range1956083/1
type: Range
persistent identity: range1956083
display id: range1956083
version: 1
direction: direction/reverse
top level: BBa_I718008
start: 1240
end: 2276
orientation: inline

BBa_I716208
type: ComponentDefinition
persistent identity: BBa I716208
display id: BBa_I716208
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [lox][terminator][lox]
creator: Samantha Liang
created: 2007-06-04T11:00:00Z
modified: 2015-08-31T04:07:51Z
discontinued: false
dominant: false
group u list: _110_
m user id: 0
owner id: 933
owning group id: 52
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: [lox][terminator][lox]
mutable notes: Digest pBca9145-I716206 (BamHI/AlwnI, 1694+553bp, Large) Digest pBca9145-Bca1114 (BglII/AlwnI, 1519+593bp, Small) Product is pBca9145-I716208.
mutable provenance: Digest pBca9145-I716206 (BamHI/AlwnI, 1694+553bp, Large) Digest pBca9145-Bca1114 (BglII/AlwnI, 1519+593bp, Small) Product is pBca9145-I716208.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence: https://synbiohub.org/public/igem/BBa_I716208_sequence/1

BBa_K1680022
type: ComponentDefinition
persistent identity: BBa K1680022
display id: BBa_K1680022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dronpa caged Cre with NLS
creator: Nicolai von Kuegelgen
created: 2015-09-16T11:00:00Z
modified: 2015-10-27T01:52:38Z
discontinued: false
dominant: false
group u list: _2098_
m user id: 15693
owner id: 26366
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding region for a fusion protein of Cre recombinase (BBa_K1680007) that is C- & N-terminal flanked with 145N Dronpa (BBa_K1680006) using a 9 AA linker as spacer (BBa_K1680001). The construct has a N-terminal NLS sequence (BBa_K1680004). In the expressed construct the two dronpa domains should form multimerize leading to an inactive Cre recombinase that is reversibly activatable through dronpa illumination (see Zhou et al 2012).
mutable notes: 3A Assembly using RFC25
mutable provenance: gBlock designed by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Cre recombinase
sequence annotation: Dronpa
sequence annotation: Linker
sequence annotation: SV40 NLS
sequence: https://synbiohub.org/public/igem/BBa_K1680022_sequence/1

Cre recombinase
type: SequenceAnnotation
persistent identity: annotation2477388
display id: annotation2477388
version: 1
top level: BBa_K1680022
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1680022/annotation2477388/range2477388/1

https://synbiohub.org/public/igem/BBa_K1680022/annotation2477388/range2477388/1
type: Range
persistent identity: range2477388
display id: range2477388
version: 1
direction: direction/forward
top level: BBa_K1680022
start: 727
end: 1755
orientation: inline

Dronpa
type: SequenceAnnotation
persistent identity: annotation2477386
display id: annotation2477386
version: 1
top level: BBa_K1680022
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1680022/annotation2477386/range2477386/1

https://synbiohub.org/public/igem/BBa_K1680022/annotation2477386/range2477386/1
type: Range
persistent identity: range2477386
display id: range2477386
version: 1
direction: direction/forward
top level: BBa_K1680022
start: 31
end: 699
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2477389
display id: annotation2477389
version: 1
top level: BBa_K1680022
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680022/annotation2477389/range2477389/1

https://synbiohub.org/public/igem/BBa_K1680022/annotation2477389/range2477389/1
type: Range
persistent identity: range2477389
display id: range2477389
version: 1
direction: direction/forward
top level: BBa_K1680022
start: 1756
end: 1783
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2477387
display id: annotation2477387
version: 1
top level: BBa_K1680022
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680022/annotation2477387/range2477387/1

https://synbiohub.org/public/igem/BBa_K1680022/annotation2477387/range2477387/1
type: Range
persistent identity: range2477387
display id: range2477387
version: 1
direction: direction/forward
top level: BBa_K1680022
start: 700
end: 726
orientation: inline

SV40 NLS
type: SequenceAnnotation
persistent identity: annotation2477385
display id: annotation2477385
version: 1
top level: BBa_K1680022
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680022/annotation2477385/range2477385/1

https://synbiohub.org/public/igem/BBa_K1680022/annotation2477385/range2477385/1
type: Range
persistent identity: range2477385
display id: range2477385
version: 1
direction: direction/forward
top level: BBa_K1680022
start: 4
end: 24
orientation: inline

Dronpa
type: SequenceAnnotation
persistent identity: annotation2477391
display id: annotation2477391
version: 1
top level: BBa_K1680022
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1680022/annotation2477391/range2477391/1

https://synbiohub.org/public/igem/BBa_K1680022/annotation2477391/range2477391/1
type: Range
persistent identity: range2477391
display id: range2477391
version: 1
direction: direction/forward
top level: BBa_K1680022
start: 1783
end: 2451
orientation: inline

Cre
type: ComponentDefinition
persistent identity: BBa J61047
display id: BBa_J61047
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cre DNA recombinase
creator: John Anderson
created: 2007-02-20T12:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: bob
mutable notes: bob
mutable provenance: bob
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_J61047_sequence/1

BBa_K1680020
type: ComponentDefinition
persistent identity: BBa K1680020
display id: BBa_K1680020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cre-dronpa fusion
creator: Nicolai von Kuegelgen
created: 2015-09-16T11:00:00Z
modified: 2016-01-26T10:16:19Z
discontinued: false
dominant: false
group u list: _2098_
m user id: 4206
owner id: 26366
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding region for a fusion construct of Cre Recombinase and 145N Dronpa with 15 amino acid linker. Oligomerisation of Dronpa should stop Cre from accessing DNA in the nucleus thereby inhibiting it until light deactivation of dronpa.
mutable notes: 3A assembly using RFC25
mutable provenance: gBlock design by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: K1680003
sequence annotation: K1680006
sequence annotation: K1680007
sequence: https://synbiohub.org/public/igem/BBa_K1680020_sequence/1

K1680003
type: SequenceAnnotation
persistent identity: annotation2477366
display id: annotation2477366
version: 1
top level: BBa_K1680020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680020/annotation2477366/range2477366/1

https://synbiohub.org/public/igem/BBa_K1680020/annotation2477366/range2477366/1
type: Range
persistent identity: range2477366
display id: range2477366
version: 1
direction: direction/forward
top level: BBa_K1680020
start: 1089
end: 1113
orientation: inline

K1680003
type: SequenceAnnotation
persistent identity: annotation2477365
display id: annotation2477365
version: 1
top level: BBa_K1680020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680020/annotation2477365/range2477365/1

https://synbiohub.org/public/igem/BBa_K1680020/annotation2477365/range2477365/1
type: Range
persistent identity: range2477365
display id: range2477365
version: 1
direction: direction/forward
top level: BBa_K1680020
start: 1
end: 39
orientation: inline

K1680006
type: SequenceAnnotation
persistent identity: annotation2477364
display id: annotation2477364
version: 1
top level: BBa_K1680020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680020/annotation2477364/range2477364/1

https://synbiohub.org/public/igem/BBa_K1680020/annotation2477364/range2477364/1
type: Range
persistent identity: range2477364
display id: range2477364
version: 1
direction: direction/forward
top level: BBa_K1680020
start: 1114
end: 1782
orientation: inline

K1680007
type: SequenceAnnotation
persistent identity: annotation2477363
display id: annotation2477363
version: 1
top level: BBa_K1680020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680020/annotation2477363/range2477363/1

https://synbiohub.org/public/igem/BBa_K1680020/annotation2477363/range2477363/1
type: Range
persistent identity: range2477363
display id: range2477363
version: 1
direction: direction/forward
top level: BBa_K1680020
start: 40
end: 1068
orientation: inline

BBa_K132027
type: ComponentDefinition
persistent identity: BBa K132027
display id: BBa_K132027
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cre induced by iptg
creator: Chen Lin,Yang Meng,Deng Ye
created: 2008-10-22T11:00:00Z
modified: 2015-05-08T01:09:51Z
discontinued: false
dominant: false
experience: experience/None
group u list: _219_
m user id: 0
owner id: 2794
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: none
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_I718007
component: BBa_B0015
component: BBa_R0010
component: BBa_I722007
sequence annotation: BBa_I718007
sequence annotation: BBa_R0010
sequence annotation: BBa_B0015
sequence annotation: BBa_I722007
sequence: https://synbiohub.org/public/igem/BBa_K132027_sequence/1

BBa_I718007
type: Component
persistent identity: component2282425
display id: component2282425
version: 1
top level: BBa_K132027
access: public
definition: BBa_I718007

BBa_B0015
type: Component
persistent identity: component2282432
display id: component2282432
version: 1
top level: BBa_K132027
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_R0010
type: Component
persistent identity: component2282414
display id: component2282414
version: 1
top level: BBa_K132027
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_I722007
type: Component
persistent identity: component2282413
display id: component2282413
version: 1
top level: BBa_K132027
access: public
definition: https://synbiohub.org/public/igem/BBa_I722007/1

BBa_I718007
type: SequenceAnnotation
persistent identity: annotation2282425
display id: annotation2282425
version: 1
top level: BBa_K132027
location: https://synbiohub.org/public/igem/BBa_K132027/annotation2282425/range2282425/1
component: BBa_I718007

https://synbiohub.org/public/igem/BBa_K132027/annotation2282425/range2282425/1
type: Range
persistent identity: range2282425
display id: range2282425
version: 1
direction: direction/reverse
top level: BBa_K132027
start: 1587
end: 2644
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2282414
display id: annotation2282414
version: 1
top level: BBa_K132027
location: https://synbiohub.org/public/igem/BBa_K132027/annotation2282414/range2282414/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K132027/annotation2282414/range2282414/1
type: Range
persistent identity: range2282414
display id: range2282414
version: 1
direction: direction/forward
top level: BBa_K132027
start: 1379
end: 1578
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2282432
display id: annotation2282432
version: 1
top level: BBa_K132027
location: https://synbiohub.org/public/igem/BBa_K132027/annotation2282432/range2282432/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K132027/annotation2282432/range2282432/1
type: Range
persistent identity: range2282432
display id: range2282432
version: 1
direction: direction/reverse
top level: BBa_K132027
start: 2653
end: 2781
orientation: inline

BBa_I722007
type: SequenceAnnotation
persistent identity: annotation2282413
display id: annotation2282413
version: 1
top level: BBa_K132027
location: https://synbiohub.org/public/igem/BBa_K132027/annotation2282413/range2282413/1
component: BBa_I722007

https://synbiohub.org/public/igem/BBa_K132027/annotation2282413/range2282413/1
type: Range
persistent identity: range2282413
display id: range2282413
version: 1
direction: direction/reverse
top level: BBa_K132027
start: 1
end: 1370
orientation: inline

BBa_K1680024
type: ComponentDefinition
persistent identity: BBa K1680024
display id: BBa_K1680024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dronpa caged Cre with NLS
creator: Nicolai von Kuegelgen
created: 2015-09-16T11:00:00Z
modified: 2016-01-26T10:22:01Z
discontinued: false
dominant: false
group u list: _2098_
m user id: 4206
owner id: 26366
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding region for a fusion protein of Cre recombinase (BBa_K1680007) that is C- & N-terminal flanked with 145N Dronpa (BBa_K1680006) using a 15 AA linker as spacer (BBa_K1680003). The construct has a N-terminal NLS sequence (BBa_K1680004). In the expressed construct the two dronpa domains should form multimerize leading to an inactive Cre recombinase that is reversibly activatable through dronpa illumination (see Zhou et al 2012).
mutable notes: 3A assembly with RFC25
mutable provenance: gBlock design by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: K1680007
sequence annotation: K1680006
sequence annotation: K1680003
sequence annotation: K1680004
sequence: https://synbiohub.org/public/igem/BBa_K1680024_sequence/1

K1680007
type: SequenceAnnotation
persistent identity: annotation2477392
display id: annotation2477392
version: 1
top level: BBa_K1680024
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680024/annotation2477392/range2477392/1

https://synbiohub.org/public/igem/BBa_K1680024/annotation2477392/range2477392/1
type: Range
persistent identity: range2477392
display id: range2477392
version: 1
direction: direction/forward
top level: BBa_K1680024
start: 745
end: 1773
orientation: inline

K1680006
type: SequenceAnnotation
persistent identity: annotation2477394
display id: annotation2477394
version: 1
top level: BBa_K1680024
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680024/annotation2477394/range2477394/1

https://synbiohub.org/public/igem/BBa_K1680024/annotation2477394/range2477394/1
type: Range
persistent identity: range2477394
display id: range2477394
version: 1
direction: direction/forward
top level: BBa_K1680024
start: 1818
end: 2487
orientation: inline

K1680006
type: SequenceAnnotation
persistent identity: annotation2477393
display id: annotation2477393
version: 1
top level: BBa_K1680024
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680024/annotation2477393/range2477393/1

https://synbiohub.org/public/igem/BBa_K1680024/annotation2477393/range2477393/1
type: Range
persistent identity: range2477393
display id: range2477393
version: 1
direction: direction/forward
top level: BBa_K1680024
start: 31
end: 699
orientation: inline

K1680003
type: SequenceAnnotation
persistent identity: annotation2477396
display id: annotation2477396
version: 1
top level: BBa_K1680024
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680024/annotation2477396/range2477396/1

https://synbiohub.org/public/igem/BBa_K1680024/annotation2477396/range2477396/1
type: Range
persistent identity: range2477396
display id: range2477396
version: 1
direction: direction/forward
top level: BBa_K1680024
start: 1774
end: 1817
orientation: inline

K1680003
type: SequenceAnnotation
persistent identity: annotation2477395
display id: annotation2477395
version: 1
top level: BBa_K1680024
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680024/annotation2477395/range2477395/1

https://synbiohub.org/public/igem/BBa_K1680024/annotation2477395/range2477395/1
type: Range
persistent identity: range2477395
display id: range2477395
version: 1
direction: direction/forward
top level: BBa_K1680024
start: 700
end: 744
orientation: inline

K1680004
type: SequenceAnnotation
persistent identity: annotation2477390
display id: annotation2477390
version: 1
top level: BBa_K1680024
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680024/annotation2477390/range2477390/1

https://synbiohub.org/public/igem/BBa_K1680024/annotation2477390/range2477390/1
type: Range
persistent identity: range2477390
display id: range2477390
version: 1
direction: direction/forward
top level: BBa_K1680024
start: 1
end: 24
orientation: inline

BBa_K112115
type: ComponentDefinition
persistent identity: BBa K112115
display id: BBa_K112115
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: a~Cre
creator: Bing Xia
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:14Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Cre recombinase gene, with a spacer for the rbs prepended. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable provenance: PCR mv025/mv002 on pBca9145-Bca1010 (1067bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112115 ----------------------------------------------- mv025 Forward Biobricking of a~Cre! ccataGAATTCatgAGATCTttaaatgtccaatttactgaccgtacacc mv002 Reverse Biobricking of <Cre! cgttaGGATCCttaatcgccatcttccagc
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K112115_sequence/1

BBa_I716213
type: ComponentDefinition
persistent identity: BBa I716213
display id: BBa_I716213
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cre (TTG start)
creator: Samantha Liang
created: 2007-06-04T11:00:00Z
modified: 2015-08-31T04:07:51Z
discontinued: false
dominant: true
experience: experience/None
group u list: _110_
m user id: 0
owner id: 933
owning group id: 52
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Cre (TTG start) related to I716212
mutable notes: PCR sil06 and G00101 on pBca9145-Bca1117 (PCR product is 1147bp, BglII/XhoI 1047 bp) Sub into pBca9145-Bca1089 (BglII/XhoI, 2063+696, L) Product is pBca9145-I716213 (TTG start) sil06 Forward BglII Cre with TTG start ctagcAGATCTttgtccaatttactgaccgtac G00101 Reverse Sequencing of pSB1A* plasmids attaccgcctttgagtgagc
mutable provenance: PCR sil06 and G00101 on pBca9145-Bca1117 (PCR product is 1147bp, BglII/XhoI 1047 bp) Sub into pBca9145-Bca1089 (BglII/XhoI, 2063+696, L) Product is pBca9145-I716213 (TTG start)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I716213_sequence/1

BBa_K132028
type: ComponentDefinition
persistent identity: BBa K132028
display id: BBa_K132028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cre terminator
creator: Deng Ye,Yang Meng,Chen Lin
created: 2008-10-22T11:00:00Z
modified: 2015-05-08T01:09:51Z
discontinued: false
dominant: false
experience: experience/None
group u list: _219_
m user id: 0
owner id: 2794
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: none
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0010
component: BBa_J61047
component: BBa_B0012
component: BBa_B0030
sequence annotation: BBa_B0030
sequence annotation: BBa_B0012
sequence annotation: BBa_J61047
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_K132028_sequence/1

BBa_B0010
type: Component
persistent identity: component1985131
display id: component1985131
version: 1
top level: BBa_K132028
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_J61047
type: Component
persistent identity: component1985130
display id: component1985130
version: 1
top level: BBa_K132028
access: public
definition: Cre

BBa_B0012
type: Component
persistent identity: component1985133
display id: component1985133
version: 1
top level: BBa_K132028
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0030
type: Component
persistent identity: component1985128
display id: component1985128
version: 1
top level: BBa_K132028
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1985128
display id: annotation1985128
version: 1
top level: BBa_K132028
location: https://synbiohub.org/public/igem/BBa_K132028/annotation1985128/range1985128/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K132028/annotation1985128/range1985128/1
type: Range
persistent identity: range1985128
display id: range1985128
version: 1
direction: direction/forward
top level: BBa_K132028
start: 1
end: 15
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1985133
display id: annotation1985133
version: 1
top level: BBa_K132028
location: https://synbiohub.org/public/igem/BBa_K132028/annotation1985133/range1985133/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K132028/annotation1985133/range1985133/1
type: Range
persistent identity: range1985133
display id: range1985133
version: 1
direction: direction/forward
top level: BBa_K132028
start: 1155
end: 1195
orientation: inline

BBa_J61047
type: SequenceAnnotation
persistent identity: annotation1985130
display id: annotation1985130
version: 1
top level: BBa_K132028
location: https://synbiohub.org/public/igem/BBa_K132028/annotation1985130/range1985130/1
component: BBa_J61047

https://synbiohub.org/public/igem/BBa_K132028/annotation1985130/range1985130/1
type: Range
persistent identity: range1985130
display id: range1985130
version: 1
direction: direction/reverse
top level: BBa_K132028
start: 22
end: 1058
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1985131
display id: annotation1985131
version: 1
top level: BBa_K132028
location: https://synbiohub.org/public/igem/BBa_K132028/annotation1985131/range1985131/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K132028/annotation1985131/range1985131/1
type: Range
persistent identity: range1985131
display id: range1985131
version: 1
direction: direction/forward
top level: BBa_K132028
start: 1067
end: 1146
orientation: inline

BBa_K1680025
type: ComponentDefinition
persistent identity: BBa K1680025
display id: BBa_K1680025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP-luciferase Cre reporter
creator: Nicolai von Kuegelgen
created: 2015-09-16T11:00:00Z
modified: 2015-10-28T03:54:52Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2098_
m user id: 15693
owner id: 26366
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part encodes a reporter cassette for the Cre Recombinase (e.g parts XYZ) in Yeast. An active Cre protein will switch expression from RFP to NanoLuc luciferase.
mutable notes: RFC10 assembly
mutable provenance: designed by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E1010
component: BBa_J63005
component: BBa_K392003
component: BBa_K1680005
component: BBa_K1680009
sequence annotation: BBa_K1680005
sequence annotation: BBa_E1010
sequence annotation: BBa_K1680009
sequence annotation: BBa_J63005
sequence annotation: BBa_K392003
sequence: https://synbiohub.org/public/igem/BBa_K1680025_sequence/1

BBa_E1010
type: Component
persistent identity: component2466050
display id: component2466050
version: 1
top level: BBa_K1680025
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_J63005
type: Component
persistent identity: component2466046
display id: component2466046
version: 1
top level: BBa_K1680025
access: public
definition: https://synbiohub.org/public/igem/BBa_J63005/1

BBa_K392003
type: Component
persistent identity: component2466051
display id: component2466051
version: 1
top level: BBa_K1680025
access: public
definition: https://synbiohub.org/public/igem/BBa_K392003/1

BBa_K1680005
type: Component
persistent identity: component2466047
display id: component2466047
version: 1
top level: BBa_K1680025
access: public
definition: BBa_K1680005

BBa_K1680005
type: Component
persistent identity: component2466052
display id: component2466052
version: 1
top level: BBa_K1680025
access: public
definition: BBa_K1680005

BBa_K1680009
type: Component
persistent identity: component2466053
display id: component2466053
version: 1
top level: BBa_K1680025
access: public
definition: https://synbiohub.org/public/igem/BBa_K1680009/1

BBa_K1680005
type: SequenceAnnotation
persistent identity: annotation2466052
display id: annotation2466052
version: 1
top level: BBa_K1680025
location: https://synbiohub.org/public/igem/BBa_K1680025/annotation2466052/range2466052/1
component: BBa_K1680005

https://synbiohub.org/public/igem/BBa_K1680025/annotation2466052/range2466052/1
type: Range
persistent identity: range2466052
display id: range2466052
version: 1
direction: direction/reverse
top level: BBa_K1680025
start: 2345
end: 2378
orientation: inline

BBa_K1680005
type: SequenceAnnotation
persistent identity: annotation2466047
display id: annotation2466047
version: 1
top level: BBa_K1680025
location: https://synbiohub.org/public/igem/BBa_K1680025/annotation2466047/range2466047/1
component: BBa_K1680005

https://synbiohub.org/public/igem/BBa_K1680025/annotation2466047/range2466047/1
type: Range
persistent identity: range2466047
display id: range2466047
version: 1
direction: direction/reverse
top level: BBa_K1680025
start: 1454
end: 1487
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2466050
display id: annotation2466050
version: 1
top level: BBa_K1680025
location: https://synbiohub.org/public/igem/BBa_K1680025/annotation2466050/range2466050/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K1680025/annotation2466050/range2466050/1
type: Range
persistent identity: range2466050
display id: range2466050
version: 1
direction: direction/reverse
top level: BBa_K1680025
start: 1494
end: 2199
orientation: inline

BBa_K1680009
type: SequenceAnnotation
persistent identity: annotation2466053
display id: annotation2466053
version: 1
top level: BBa_K1680025
location: https://synbiohub.org/public/igem/BBa_K1680025/annotation2466053/range2466053/1
component: BBa_K1680009

https://synbiohub.org/public/igem/BBa_K1680025/annotation2466053/range2466053/1
type: Range
persistent identity: range2466053
display id: range2466053
version: 1
direction: direction/reverse
top level: BBa_K1680025
start: 2385
end: 2900
orientation: inline

BBa_J63005
type: SequenceAnnotation
persistent identity: annotation2466046
display id: annotation2466046
version: 1
top level: BBa_K1680025
location: https://synbiohub.org/public/igem/BBa_K1680025/annotation2466046/range2466046/1
component: BBa_J63005

https://synbiohub.org/public/igem/BBa_K1680025/annotation2466046/range2466046/1
type: Range
persistent identity: range2466046
display id: range2466046
version: 1
direction: direction/reverse
top level: BBa_K1680025
start: 1
end: 1445
orientation: inline

BBa_K392003
type: SequenceAnnotation
persistent identity: annotation2466051
display id: annotation2466051
version: 1
top level: BBa_K1680025
location: https://synbiohub.org/public/igem/BBa_K1680025/annotation2466051/range2466051/1
component: BBa_K392003

https://synbiohub.org/public/igem/BBa_K1680025/annotation2466051/range2466051/1
type: Range
persistent identity: range2466051
display id: range2466051
version: 1
direction: direction/reverse
top level: BBa_K1680025
start: 2208
end: 2336
orientation: inline

BBa_K1680017
type: ComponentDefinition
persistent identity: BBa K1680017
display id: BBa_K1680017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cre-Dronpa Fusion
creator: Nicolai von K??gelgen
created: 2015-09-16T11:00:00Z
modified: 2016-01-26T10:15:17Z
discontinued: false
dominant: false
group u list: _2098_
m user id: 4206
owner id: 26366
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding region for a fusion construct of Cre Recombinase and 145N Dronpa with intermediate 6 amino acid linker. Oligomerisation of Dronpa should stop Cre from accessing DNA in the nucleus thereby inhibiting it until light deactivation of dronpa.
mutable notes: Assembled using 3A assembly.
mutable provenance: gBlocks designed by Team Tuebingen.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_K1680006
sequence annotation: BBa_K1680000
sequence annotation: BBa_K1680007
sequence: https://synbiohub.org/public/igem/BBa_K1680017_sequence/1

BBa_K1680006
type: SequenceAnnotation
persistent identity: annotation2465979
display id: annotation2465979
version: 1
top level: BBa_K1680017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1680017/annotation2465979/range2465979/1

https://synbiohub.org/public/igem/BBa_K1680017/annotation2465979/range2465979/1
type: Range
persistent identity: range2465979
display id: range2465979
version: 1
direction: direction/reverse
top level: BBa_K1680017
start: 1064
end: 1732
orientation: inline

BBa_K1680000
type: SequenceAnnotation
persistent identity: annotation2465976
display id: annotation2465976
version: 1
top level: BBa_K1680017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1680017/annotation2465976/range2465976/1

https://synbiohub.org/public/igem/BBa_K1680017/annotation2465976/range2465976/1
type: Range
persistent identity: range2465976
display id: range2465976
version: 1
direction: direction/reverse
top level: BBa_K1680017
start: 1
end: 6
orientation: inline

BBa_K1680007
type: SequenceAnnotation
persistent identity: annotation2465977
display id: annotation2465977
version: 1
top level: BBa_K1680017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1680017/annotation2465977/range2465977/1

https://synbiohub.org/public/igem/BBa_K1680017/annotation2465977/range2465977/1
type: Range
persistent identity: range2465977
display id: range2465977
version: 1
direction: direction/reverse
top level: BBa_K1680017
start: 13
end: 1041
orientation: inline

BBa_K1680000
type: SequenceAnnotation
persistent identity: annotation2465978
display id: annotation2465978
version: 1
top level: BBa_K1680017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1680017/annotation2465978/range2465978/1

https://synbiohub.org/public/igem/BBa_K1680017/annotation2465978/range2465978/1
type: Range
persistent identity: range2465978
display id: range2465978
version: 1
direction: direction/reverse
top level: BBa_K1680017
start: 1050
end: 1055
orientation: inline

BBa_K1680023
type: ComponentDefinition
persistent identity: BBa K1680023
display id: BBa_K1680023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dronpa caged Cre with NLS
creator: Nicolai von Kuegelgen
created: 2015-09-16T11:00:00Z
modified: 2016-01-26T10:42:40Z
discontinued: false
dominant: false
group u list: _2098_
m user id: 4206
owner id: 26366
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding region for a fusion protein of Cre recombinase (BBa_K1680007) that is C- & N-terminal flanked with 145N Dronpa (BBa_K1680006) using a 12 AA linker as spacer (BBa_K1680002). The construct has a N-terminal NLS sequence (BBa_K1680004). In the expressed construct the two dronpa domains should form multimerize leading to an inactive Cre recombinase that is reversibly activatable through dronpa illumination (see Zhou et al 2012).
mutable notes: 3A assembly using RFC25
mutable provenance: gBlock design by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: K1680002
sequence annotation: K1680006
sequence annotation: K1680007
sequence annotation: K1680004
sequence: https://synbiohub.org/public/igem/BBa_K1680023_sequence/1

K1680002
type: SequenceAnnotation
persistent identity: annotation2477384
display id: annotation2477384
version: 1
top level: BBa_K1680023
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680023/annotation2477384/range2477384/1

https://synbiohub.org/public/igem/BBa_K1680023/annotation2477384/range2477384/1
type: Range
persistent identity: range2477384
display id: range2477384
version: 1
direction: direction/forward
top level: BBa_K1680023
start: 1765
end: 1800
orientation: inline

K1680006
type: SequenceAnnotation
persistent identity: annotation2477380
display id: annotation2477380
version: 1
top level: BBa_K1680023
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680023/annotation2477380/range2477380/1

https://synbiohub.org/public/igem/BBa_K1680023/annotation2477380/range2477380/1
type: Range
persistent identity: range2477380
display id: range2477380
version: 1
direction: direction/forward
top level: BBa_K1680023
start: 31
end: 699
orientation: inline

K1680007
type: SequenceAnnotation
persistent identity: annotation2477382
display id: annotation2477382
version: 1
top level: BBa_K1680023
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680023/annotation2477382/range2477382/1

https://synbiohub.org/public/igem/BBa_K1680023/annotation2477382/range2477382/1
type: Range
persistent identity: range2477382
display id: range2477382
version: 1
direction: direction/forward
top level: BBa_K1680023
start: 736
end: 1764
orientation: inline

K1680002
type: SequenceAnnotation
persistent identity: annotation2477383
display id: annotation2477383
version: 1
top level: BBa_K1680023
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680023/annotation2477383/range2477383/1

https://synbiohub.org/public/igem/BBa_K1680023/annotation2477383/range2477383/1
type: Range
persistent identity: range2477383
display id: range2477383
version: 1
direction: direction/forward
top level: BBa_K1680023
start: 700
end: 735
orientation: inline

K1680004
type: SequenceAnnotation
persistent identity: annotation2477379
display id: annotation2477379
version: 1
top level: BBa_K1680023
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680023/annotation2477379/range2477379/1

https://synbiohub.org/public/igem/BBa_K1680023/annotation2477379/range2477379/1
type: Range
persistent identity: range2477379
display id: range2477379
version: 1
direction: direction/forward
top level: BBa_K1680023
start: 1
end: 24
orientation: inline

K1680006
type: SequenceAnnotation
persistent identity: annotation2477381
display id: annotation2477381
version: 1
top level: BBa_K1680023
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680023/annotation2477381/range2477381/1

https://synbiohub.org/public/igem/BBa_K1680023/annotation2477381/range2477381/1
type: Range
persistent identity: range2477381
display id: range2477381
version: 1
direction: direction/forward
top level: BBa_K1680023
start: 1801
end: 2469
orientation: inline

BBa_K082011
type: ComponentDefinition
persistent identity: BBa K082011
display id: BBa_K082011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034-J61047
creator: Xunyao Wu
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _228_
m user id: 0
owner id: 1560
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: none
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0034
component: BBa_J61047
sequence annotation: BBa_B0034
sequence annotation: BBa_J61047
sequence: https://synbiohub.org/public/igem/BBa_K082011_sequence/1

BBa_B0034
type: Component
persistent identity: component1993202
display id: component1993202
version: 1
top level: BBa_K082011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_J61047
type: Component
persistent identity: component1993203
display id: component1993203
version: 1
top level: BBa_K082011
access: public
definition: Cre

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1993202
display id: annotation1993202
version: 1
top level: BBa_K082011
location: https://synbiohub.org/public/igem/BBa_K082011/annotation1993202/range1993202/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K082011/annotation1993202/range1993202/1
type: Range
persistent identity: range1993202
display id: range1993202
version: 1
direction: direction/reverse
top level: BBa_K082011
start: 1
end: 12
orientation: inline

BBa_J61047
type: SequenceAnnotation
persistent identity: annotation1993203
display id: annotation1993203
version: 1
top level: BBa_K082011
location: https://synbiohub.org/public/igem/BBa_K082011/annotation1993203/range1993203/1
component: BBa_J61047

https://synbiohub.org/public/igem/BBa_K082011/annotation1993203/range1993203/1
type: Range
persistent identity: range1993203
display id: range1993203
version: 1
direction: direction/reverse
top level: BBa_K082011
start: 19
end: 1055
orientation: inline

BBa_K1926011
type: ComponentDefinition
persistent identity: BBa K1926011
display id: BBa_K1926011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The SNAP UNIT: SNAP-tag flanked by loxP
creator: Xinyi Liu
created: 2016-10-08T11:00:00Z
modified: 2016-10-19T01:22:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2393_
m user id: 28998
owner id: 28998
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part is the SNAP UNIT of cyclebow system including an sv40 nuclear location sequence (NLS), a covalent labeling of fusion proteins named SNAP-tag?? and an sv40 terminator flanked by homodromous recognition site of recombinase CRE named loxP. The whole part can be cut off by CRE. You may use this part to: 1) Localize proteins in vivo with BLOCK and dye from NEB company; 2) Dye after blocking, you are able to see whether an event have an influence on the localization and expression of the interested protein; 3) Fast cut off this part by transient transfecting recombinase gene into nucleus.
mutable notes: None
mutable provenance: The SNAP-tag?? was brought from NEB company. NLS and sv40 terminator were got from commercial plasmid: pentry nls GFP/pcdna3.1.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: SNAP-tag
sequence annotation: loxP
sequence annotation: sv40 terminator
sequence annotation: TAA
sequence annotation: NLS
sequence: https://synbiohub.org/public/igem/BBa_K1926011_sequence/1

SNAP-tag
type: SequenceAnnotation
persistent identity: annotation2491738
display id: annotation2491738
version: 1
top level: BBa_K1926011
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1926011/annotation2491738/range2491738/1

https://synbiohub.org/public/igem/BBa_K1926011/annotation2491738/range2491738/1
type: Range
persistent identity: range2491738
display id: range2491738
version: 1
direction: direction/forward
top level: BBa_K1926011
start: 59
end: 604
orientation: inline

loxP
type: SequenceAnnotation
persistent identity: annotation2491776
display id: annotation2491776
version: 1
top level: BBa_K1926011
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1926011/annotation2491776/range2491776/1

https://synbiohub.org/public/igem/BBa_K1926011/annotation2491776/range2491776/1
type: Range
persistent identity: range2491776
display id: range2491776
version: 1
direction: direction/forward
top level: BBa_K1926011
start: 755
end: 788
orientation: inline

sv40 terminator
type: SequenceAnnotation
persistent identity: annotation2491779
display id: annotation2491779
version: 1
top level: BBa_K1926011
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1926011/annotation2491779/range2491779/1

https://synbiohub.org/public/igem/BBa_K1926011/annotation2491779/range2491779/1
type: Range
persistent identity: range2491779
display id: range2491779
version: 1
direction: direction/forward
top level: BBa_K1926011
start: 605
end: 754
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2491851
display id: annotation2491851
version: 1
top level: BBa_K1926011
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1926011/annotation2491851/range2491851/1

https://synbiohub.org/public/igem/BBa_K1926011/annotation2491851/range2491851/1
type: Range
persistent identity: range2491851
display id: range2491851
version: 1
direction: direction/forward
top level: BBa_K1926011
start: 626
end: 628
orientation: inline

NLS
type: SequenceAnnotation
persistent identity: annotation2491778
display id: annotation2491778
version: 1
top level: BBa_K1926011
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1926011/annotation2491778/range2491778/1

https://synbiohub.org/public/igem/BBa_K1926011/annotation2491778/range2491778/1
type: Range
persistent identity: range2491778
display id: range2491778
version: 1
direction: direction/forward
top level: BBa_K1926011
start: 38
end: 58
orientation: inline

loxP
type: SequenceAnnotation
persistent identity: annotation2491774
display id: annotation2491774
version: 1
top level: BBa_K1926011
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1926011/annotation2491774/range2491774/1

https://synbiohub.org/public/igem/BBa_K1926011/annotation2491774/range2491774/1
type: Range
persistent identity: range2491774
display id: range2491774
version: 1
direction: direction/forward
top level: BBa_K1926011
start: 1
end: 34
orientation: inline

BBa_K1680019
type: ComponentDefinition
persistent identity: BBa K1680019
display id: BBa_K1680019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cre-dronpa fusion
creator: Nicolai von Kuegelgen
created: 2015-09-16T11:00:00Z
modified: 2016-01-26T10:15:55Z
discontinued: false
dominant: false
group u list: _2098_
m user id: 4206
owner id: 26366
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding region for a fusion construct of Cre Recombinase and 145N Dronpa with 12 amino acid linker. Oligomerisation of Dronpa should stop Cre from accessing DNA in the nucleus thereby inhibiting it until light deactivation of dronpa.
mutable notes: 3A assembly using RFC25
mutable provenance: gBlocks designed by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: K1680002
sequence annotation: K1680007
sequence annotation: K1680006
sequence: https://synbiohub.org/public/igem/BBa_K1680019_sequence/1

K1680002
type: SequenceAnnotation
persistent identity: annotation2477417
display id: annotation2477417
version: 1
top level: BBa_K1680019
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680019/annotation2477417/range2477417/1

https://synbiohub.org/public/igem/BBa_K1680019/annotation2477417/range2477417/1
type: Range
persistent identity: range2477417
display id: range2477417
version: 1
direction: direction/forward
top level: BBa_K1680019
start: 1060
end: 1095
orientation: inline

K1680007
type: SequenceAnnotation
persistent identity: annotation2477416
display id: annotation2477416
version: 1
top level: BBa_K1680019
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680019/annotation2477416/range2477416/1

https://synbiohub.org/public/igem/BBa_K1680019/annotation2477416/range2477416/1
type: Range
persistent identity: range2477416
display id: range2477416
version: 1
direction: direction/forward
top level: BBa_K1680019
start: 31
end: 1059
orientation: inline

K1680002
type: SequenceAnnotation
persistent identity: annotation2477415
display id: annotation2477415
version: 1
top level: BBa_K1680019
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680019/annotation2477415/range2477415/1

https://synbiohub.org/public/igem/BBa_K1680019/annotation2477415/range2477415/1
type: Range
persistent identity: range2477415
display id: range2477415
version: 1
direction: direction/forward
top level: BBa_K1680019
start: 1
end: 30
orientation: inline

K1680006
type: SequenceAnnotation
persistent identity: annotation2477418
display id: annotation2477418
version: 1
top level: BBa_K1680019
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680019/annotation2477418/range2477418/1

https://synbiohub.org/public/igem/BBa_K1680019/annotation2477418/range2477418/1
type: Range
persistent identity: range2477418
display id: range2477418
version: 1
direction: direction/forward
top level: BBa_K1680019
start: 1096
end: 1764
orientation: inline

BBa_K416002
type: ComponentDefinition
persistent identity: BBa K416002
display id: BBa_K416002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 36 Base Pair LoxP
creator: Russell Durrett
created: 2010-05-31T11:00:00Z
modified: 2015-05-08T01:12:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _527_
m user id: 0
owner id: 6462
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The antibody for the c-myc transcription factor. This protein binds to Enhancer Box sequences and recruits histone acetyltransferases, serving as a traditional transcription factor and a regulator of global chromatin structure.
mutable notes: The coding region of the gene was isolated via PCR. Biobrick prefix and suffix were added during amplification, and ligated into Ampicillin resistant plasmids.
mutable provenance: Amplified and biobricked from E. coli.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K416002_sequence/1

BBa_K1680018
type: ComponentDefinition
persistent identity: BBa K1680018
display id: BBa_K1680018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cre-Dronpa fusion
creator: Nicolai von K??gelgen
created: 2015-09-16T11:00:00Z
modified: 2016-01-26T10:15:36Z
discontinued: false
dominant: false
group u list: _2098_
m user id: 4206
owner id: 26366
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding region for a fusion construct of Cre Recombinase and 145N Dronpa with 9 amino acid linker. Oligomerisation of Dronpa should stop Cre from accessing DNA in the nucleus thereby inhibiting it until light deactivation of dronpa.
mutable notes: Assembled using 3A assembly and RFC25.
mutable provenance: gBlocks designed by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: K1680001
sequence annotation: K1680007
sequence annotation: K1680006
sequence: https://synbiohub.org/public/igem/BBa_K1680018_sequence/1

K1680001
type: SequenceAnnotation
persistent identity: annotation2477358
display id: annotation2477358
version: 1
top level: BBa_K1680018
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680018/annotation2477358/range2477358/1

https://synbiohub.org/public/igem/BBa_K1680018/annotation2477358/range2477358/1
type: Range
persistent identity: range2477358
display id: range2477358
version: 1
direction: direction/forward
top level: BBa_K1680018
start: 1051
end: 1077
orientation: inline

K1680001
type: SequenceAnnotation
persistent identity: annotation2477357
display id: annotation2477357
version: 1
top level: BBa_K1680018
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680018/annotation2477357/range2477357/1

https://synbiohub.org/public/igem/BBa_K1680018/annotation2477357/range2477357/1
type: Range
persistent identity: range2477357
display id: range2477357
version: 1
direction: direction/forward
top level: BBa_K1680018
start: 1
end: 21
orientation: inline

K1680007
type: SequenceAnnotation
persistent identity: annotation2477355
display id: annotation2477355
version: 1
top level: BBa_K1680018
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680018/annotation2477355/range2477355/1

https://synbiohub.org/public/igem/BBa_K1680018/annotation2477355/range2477355/1
type: Range
persistent identity: range2477355
display id: range2477355
version: 1
direction: direction/forward
top level: BBa_K1680018
start: 22
end: 1050
orientation: inline

K1680006
type: SequenceAnnotation
persistent identity: annotation2477354
display id: annotation2477354
version: 1
top level: BBa_K1680018
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680018/annotation2477354/range2477354/1

https://synbiohub.org/public/igem/BBa_K1680018/annotation2477354/range2477354/1
type: Range
persistent identity: range2477354
display id: range2477354
version: 1
direction: direction/forward
top level: BBa_K1680018
start: 1078
end: 1746
orientation: inline

BBa_K112101
type: ComponentDefinition
persistent identity: BBa K112101
display id: BBa_K112101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cre enzyme without stop codon
creator: Madhvi Venkatesh
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:13Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 3023
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Cre enzyme without stop codon
mutable notes: This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites. --------------------------------------------- PCR mv003/mv004 on pBca9145-Bca1010 (1060bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112101 ----------------------------------------------- mv003 Forward Biobricking of Cre> ccataGAATTCatgAGATCTatgtccaatttactgaccgtac mv004 Reverse Biobricking of Cre> cgttaGGATCCatcgccatcttccagcaggcg <//pre>
mutable provenance: PCR mv003/mv004 on pBca9145-Bca1010 (1060bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112101
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K112101_sequence/1

BBa_K112100
type: ComponentDefinition
persistent identity: BBa K112100
display id: BBa_K112100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cre enzyme without start codon
creator: Madhvi Venkatesh
created: 2008-08-13T11:00:00Z
modified: 2015-05-08T01:09:13Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 3023
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Cre enzyme without start codon
mutable notes: This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites. PCR mv001/mv002 on pBca9145-Bca1010 (1060bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112100 ----------------------------------------------- mv001 Forward Biobricking of <Cre! ccataGAATTCatgAGATCTtccaatttactgaccgtacacc mv002 Reverse Biobricking of <Cre! cgttaGGATCCttaatcgccatcttccagc
mutable provenance: PCR mv001/mv002 on pBca9145-Bca1010 (1060bp, EcoRI/BamHI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112100
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K112100_sequence/1

BBa_K886003
type: ComponentDefinition
persistent identity: BBa K886003
display id: BBa_K886003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Recombination Device composed by Cre-lox71
creator: edgar andres ochoa cruz
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _940_
m user id: 0
owner id: 11298
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This device was created to express the Cre recombinase enzyme and a target gene under the control of the same promoter (promoter not included in this part). The target gene needs to be amplified by PCR, using primers that will insert a loxP and a lox66 sites flanking the sequence, in order to integrate it in the lox71 recombination site. For this part the lox71 is located downstream the Cre recombinase gene. An alternative construction is submitted in the part BBa_K886001. Once this device is located under the control of the desired promoter, it will express the Cre recombinase that recognizes the lox sites and inserts the target gene under the control of the same promoter. The Cre expression levels are critical for the recombination, we created two different devices: BBa_K886002 (Cre-Lox71) and BBa_K886001 (Lox71-Cre) for testing if inserting the target gene either upstream or downstream the Cre gene will affect the expression efficiency of the target gene
mutable notes: We used the lox71 submitted by our group BBa_K886000, which corrected the parts BBa_I718017 and BBa_K537020. Those parts are wrong when compared with the published sequence at http://www.ncbi.nlm.nih.gov/pmc/articles/PMC137435/
mutable provenance: We synthesized the lox71 site, then we assembled it downstream the Cre recombinase gene(BBa_J61047).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_J61047
component: BBa_K886000
sequence annotation: BBa_K886000
sequence annotation: BBa_J61047
sequence: https://synbiohub.org/public/igem/BBa_K886003_sequence/1

BBa_J61047
type: Component
persistent identity: component2196284
display id: component2196284
version: 1
top level: BBa_K886003
access: public
definition: Cre

BBa_K886000
type: Component
persistent identity: component2196285
display id: component2196285
version: 1
top level: BBa_K886003
access: public
definition: BBa_K886000

BBa_K886000
type: SequenceAnnotation
persistent identity: annotation2196285
display id: annotation2196285
version: 1
top level: BBa_K886003
location: https://synbiohub.org/public/igem/BBa_K886003/annotation2196285/range2196285/1
component: BBa_K886000

https://synbiohub.org/public/igem/BBa_K886003/annotation2196285/range2196285/1
type: Range
persistent identity: range2196285
display id: range2196285
version: 1
direction: direction/reverse
top level: BBa_K886003
start: 1046
end: 1079
orientation: inline

BBa_J61047
type: SequenceAnnotation
persistent identity: annotation2196284
display id: annotation2196284
version: 1
top level: BBa_K886003
location: https://synbiohub.org/public/igem/BBa_K886003/annotation2196284/range2196284/1
component: BBa_J61047

https://synbiohub.org/public/igem/BBa_K886003/annotation2196284/range2196284/1
type: Range
persistent identity: range2196284
display id: range2196284
version: 1
direction: direction/reverse
top level: BBa_K886003
start: 1
end: 1037
orientation: inline

cre
type: ComponentDefinition
persistent identity: BBa K112102
display id: BBa_K112102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cre enzyme without start or stop codons
creator: Madhvi Venkatesh
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:13Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 3023
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Cre enzyme without start or stop codons
mutable notes: This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites. ---------------------------------------- PCR mv001/mv004 on pBca9145-Bca1010 (1057bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112102 ----------------------------------------------- mv001 Forward Biobricking of <Cre! ccataGAATTCatgAGATCTtccaatttactgaccgtacacc mv004 Reverse Biobricking of Cre> cgttaGGATCCatcgccatcttccagcaggcg
mutable provenance: PCR mv001/mv004 on pBca9145-Bca1010 (1057bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112102
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K112102_sequence/1

BBa_K886000
type: ComponentDefinition
persistent identity: BBa K886000
display id: BBa_K886000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fixed lox71
creator: edgar andres ochoa cruz
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1149_
m user id: 0
owner id: 11298
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The particularity of lox71 is that it has an altered sequence at the end of it's right arm compared to loxP. This sequence variation reduces affinity of the Cre recombinase for the arm.
mutable notes: Based on the report made by the igem2010 UT-Tokyo team and some papers, the lox71 (BBa_I718017) from the registry was wrong, it had a cg instead a gc in its center sequence that is between the arms. Apparently, this part was corrected by the iGEM11_WITS_CSIR_SA team (BBa_K537020), but the sequence from this group had the same error. The corrected part from iGEM11_Tokyo_Tech (BBa_K649205) was right but no DNA was available in the registry. So we decided to synthesized it and test it, using the proper sequence described by http://www.ncbi.nlm.nih.gov/pmc/articles/PMC137435/, and we submitted the correct DNA to the Registry.
mutable provenance: primers
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K886000_sequence/1

RBS.cre
type: ComponentDefinition
persistent identity: BBa I716436
display id: BBa_I716436
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {rbs1-A}{GTG.Cre}
creator: Samantha Liang
created: 2007-10-22T11:00:00Z
modified: 2015-08-31T04:07:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _110_
m user id: 0
owner id: 933
owning group id: 52
sample status: In stock
status: status/Available
bookmark: false
mutable description: {rbs1-A}{GTG.Cre}
mutable notes: Bca1106-A + I716212
mutable provenance: Bca1106-A + I716212
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_I716436_sequence/1

BBa_K1680007
type: ComponentDefinition
persistent identity: BBa K1680007
display id: BBa_K1680007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cre recombinase
creator: Katharina Sporbeck
created: 2015-09-16T11:00:00Z
modified: 2015-10-27T01:34:10Z
discontinued: false
dominant: false
group u list: _2098_
m user id: 15693
owner id: 26460
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding region for the DNA recombinase Cre, codon optimized for yeast. The Cre recombinase recognizes two loxP sites (Part: BBa_K1680005) and depending on their orientation cuts out or reverses the DNA sequence between them.
mutable notes: Part is shipped in RFC25.
mutable provenance: gBlock designed by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Cre recombinase
sequence: https://synbiohub.org/public/igem/BBa_K1680007_sequence/1

Cre recombinase
type: SequenceAnnotation
persistent identity: annotation2477356
display id: annotation2477356
version: 1
top level: BBa_K1680007
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680007/annotation2477356/range2477356/1

https://synbiohub.org/public/igem/BBa_K1680007/annotation2477356/range2477356/1
type: Range
persistent identity: range2477356
display id: range2477356
version: 1
direction: direction/forward
top level: BBa_K1680007
start: 1
end: 1029
orientation: inline

Cre
type: ComponentDefinition
persistent identity: BBa I716438
display id: BBa_I716438
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {rbs1-A}{GTG.Cre}{b0015 TT}
creator: Samantha Liang
created: 2007-10-22T11:00:00Z
modified: 2015-08-31T04:07:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _110_
m user id: 0
owner id: 933
owning group id: 52
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: {rbs1-A}{GTG.Cre}{b0015 TT}
mutable notes: the Cre has a GTG start to weaken it's activity
mutable provenance: I716436 + Bca1092
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence: https://synbiohub.org/public/igem/BBa_I716438_sequence/1

lox71
type: ComponentDefinition
persistent identity: BBa I718017
display id: BBa_I718017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lox71
creator: Eimad Shotar
created: 2007-10-25T11:00:00Z
modified: 2015-08-31T04:07:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _141_
m user id: 0
owner id: 1568
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Lox71 is a site specific recombination cassette. It belongs to the loxP family frequently used in genetics, particularly in mouse genetics. lox site recombination is catalysed by a Site specific recombinase, Cre. lox sequences are composed of an 8 bp Core sequence surrounded by two Arms. The particularity of lox66 is that it has an altered sequence at the end of it's left arm compared to loxP. This sequence variation reduces affinity of the Cre recombinase for the arm. As a consequence, after a recombination between a lox71 and a lox66 (altered right arm sequence), one of the two resulting generated lox sites has very low recombination potential as it inherited both mutated arms. Use of lox71 & lox66 sites is potentially interesting when the recombination reaction must be "irreversible".
mutable notes: No modifaication was made on lox71 sequence
mutable provenance: This part was generated in the form of a forward & a reverse primer. After annealing these primers EcoRI & PstI compatible cohesive ends at the 5' & 3' ends of the dsDNA were generated. Next, the dsDNA was subcloned in a pSB1A2 open plasmid (digested with EcoRI & PstI) You can follow the construction process by following the links available in the Paris iGEM 2007 wiki: http://parts.mit.edu/igem07/index.php/Paris "freezer" section plasmids table. A links sends you to the corresponding notebook date when the ligation reaction was performed
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_I718017_sequence/1

lox66
type: ComponentDefinition
persistent identity: BBa I718016
display id: BBa_I718016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lox66
creator: Eimad Shotar
created: 2007-10-25T11:00:00Z
modified: 2015-08-31T04:07:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _141_
m user id: 0
owner id: 1568
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: lox66 is a site specific recombination cassette. It belongs to the loxP family frequently used in genetics, particularily in mouse genetics. lox site recombination is catalysed by a Site specific recombinase, Cre. lox sequences are composed of an 8 bp Core sequence surrounded by two Arms. The particularity of lox66 is that it has an altered sequence at the end of it's left arm compared to loxP. This sequence variation reduces affinity of the Cre recombinase for the arm. As a consequence, after a recombination between a lox66 and a lox71 (altered right arm sequence), one of the two resulting generated lox sites has very low recombination potential as it inherited both mutated arms. Use of lox66 & lox71 sites is potentially interresting when the recombination reaction must be "irreversible".
mutable notes: No modidification was made on the lox66 sequence
mutable provenance: This part was generated in the form of a forward & a reverse primer. After annealing these primers EcoRI & PstI compatible cohesive ends at the 5' & 3' ends of the dsDNA were generated. Next, the dsDNA was subcloned in a pSB1A2 open plasmid (digested with EcoRI & PstI) You can follow the construction process by following the links available in the Paris iGEM 2007 wiki: http://parts.mit.edu/igem07/index.php/Paris "freezer" section plasmids table. A links sends you to the corresponding notebook date when the ligation reaction was performed
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_I718016_sequence/1

BBa_K886001
type: ComponentDefinition
persistent identity: BBa K886001
display id: BBa_K886001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Recombination Device composed by lox71-Cre recombinase
creator: edgar andres ochoa cruz
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1149_
m user id: 0
owner id: 11298
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: This device was created to express the Cre recombinase enzyme and a target gene under the control of the same promoter (promoter not included in this part). The target gene needs to be amplified by PCR using primers that will insert a loxP and a lox66 sites flanking the sequence in order to integrate it in the lox71 recombination site located upstream the Cre recombinase gene. Once this device is located under the control of the desired promoter, it will express the Cre recombinase that recognizes the lox sites and inserts the target gene under the control of the same promoter. The Cre expression levels are critical for the recombination, we created two different devices: A (Cre-Lox71) and B (Lox71-Cre) for testing if inserting the target gene either upstream or downstream the Cre gene will affect the expression efficiency of the target gene.
mutable notes: We used the lox71 submitted by our group, which correct the part BBa_I718017 and BBa_K537020 when compared with the published sequence at http://www.ncbi.nlm.nih.gov/pmc/articles/PMC137435/
mutable provenance: We synthesized the lox71 site and then we assembled it with the Cre recombinase part BBa_J61047
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K886000
component: BBa_J61047
sequence annotation: BBa_K886000
sequence annotation: BBa_J61047
sequence: https://synbiohub.org/public/igem/BBa_K886001_sequence/1

BBa_K886000
type: Component
persistent identity: component2196193
display id: component2196193
version: 1
top level: BBa_K886001
access: public
definition: BBa_K886000

BBa_J61047
type: Component
persistent identity: component2196194
display id: component2196194
version: 1
top level: BBa_K886001
access: public
definition: Cre

BBa_K886000
type: SequenceAnnotation
persistent identity: annotation2196193
display id: annotation2196193
version: 1
top level: BBa_K886001
location: https://synbiohub.org/public/igem/BBa_K886001/annotation2196193/range2196193/1
component: BBa_K886000

https://synbiohub.org/public/igem/BBa_K886001/annotation2196193/range2196193/1
type: Range
persistent identity: range2196193
display id: range2196193
version: 1
direction: direction/reverse
top level: BBa_K886001
start: 1
end: 34
orientation: inline

BBa_J61047
type: SequenceAnnotation
persistent identity: annotation2196194
display id: annotation2196194
version: 1
top level: BBa_K886001
location: https://synbiohub.org/public/igem/BBa_K886001/annotation2196194/range2196194/1
component: BBa_J61047

https://synbiohub.org/public/igem/BBa_K886001/annotation2196194/range2196194/1
type: Range
persistent identity: range2196194
display id: range2196194
version: 1
direction: direction/reverse
top level: BBa_K886001
start: 41
end: 1077
orientation: inline

BBa_I716212
type: ComponentDefinition
persistent identity: BBa I716212
display id: BBa_I716212
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cre (GTG start)
creator: Samantha Liang
created: 2007-06-04T11:00:00Z
modified: 2015-08-31T04:07:51Z
discontinued: false
dominant: true
experience: experience/None
group u list: _110_
m user id: 0
owner id: 933
owning group id: 52
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: weakened version of Cre (has a GTG start codon) because we want a lower copy
mutable notes: PCR sil05 and G00101 on pBca9145-Bca1117 (PCR product is 1147bp, BglII/XhoI 1047 bp) Sub into pBca9145-Bca1089 (BglII/XhoI, 2063+696, L) Product is pBca9145-I716212 (GTG start) sil05 Forward BglII Cre with GTG start ctagcAGATCTgtgtccaatttactgaccgtac G00101 Reverse Sequencing of pSB1A* plasmids attaccgcctttgagtgagc
mutable provenance: PCR sil05 and G00101 on pBca9145-Bca1117 (PCR product is 1147bp, BglII/XhoI 1047 bp) Sub into pBca9145-Bca1089 (BglII/XhoI, 2063+696, L) Product is pBca9145-I716212 (GTG start) sil05 Forward BglII Cre with GTG start ctagcAGATCTgtgtccaatttactgaccgtac G00101 Reverse Sequencing of pSB1A* plasmids attaccgcctttgagtgagc
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I716212_sequence/1

BBa_K886002
type: ComponentDefinition
persistent identity: BBa K886002
display id: BBa_K886002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Recombination Device composed by Cre-lox71
creator: edgar andres ochoa cruz
created: 2012-09-23T11:00:00Z
is replaced by: BBa_K886001
modified: 2015-05-08T01:13:40Z
discontinued: true
dominant: false
experience: experience/None
group u list: _1149_
m user id: 0
owner id: 11298
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: one
mutable notes: None
mutable provenance: composite
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K886001
sequence annotation: BBa_K886001
sequence: https://synbiohub.org/public/igem/BBa_K886002_sequence/1

BBa_K886001
type: Component
persistent identity: component2196198
display id: component2196198
version: 1
top level: BBa_K886002
access: public
definition: BBa_K886001

BBa_K886001
type: SequenceAnnotation
persistent identity: annotation2196198
display id: annotation2196198
version: 1
top level: BBa_K886002
location: https://synbiohub.org/public/igem/BBa_K886002/annotation2196198/range2196198/1
component: BBa_K886001

https://synbiohub.org/public/igem/BBa_K886002/annotation2196198/range2196198/1
type: Range
persistent identity: range2196198
display id: range2196198
version: 1
direction: direction/reverse
top level: BBa_K886002
start: 1
end: 1077
orientation: inline

lox
type: ComponentDefinition
persistent identity: BBa J61046
display id: BBa_J61046
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [Lox] site for recombination
creator: John Anderson
created: 2007-02-20T12:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: bob
mutable notes: bob
mutable provenance: bob
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_J61046_sequence/1

BBa_K1680021
type: ComponentDefinition
persistent identity: BBa K1680021
display id: BBa_K1680021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dronpa caged Cre with NLS
creator: Nicolai von Kuegelgen
created: 2015-09-16T11:00:00Z
modified: 2016-01-26T01:27:03Z
discontinued: false
dominant: false
group u list: _2098_
m user id: 4206
owner id: 26366
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding region for a fusion protein of Cre recombinase (BBa_K1680007) that is C- & N-terminal flanked with 145N Dronpa (BBa_K1680006) using a 6 AA linker as spacer (BBa_K1680000). The construct has a N-terminal NLS sequence (BBa_K1680004). In the expressed construct the two dronpa domains should form multimerize leading to an inactive Cre recombinase that is reversibly activatable through dronpa illumination (see Zhou et al 2012).
mutable notes: 3A Assembly using RFC25
mutable provenance: gBlock design by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Dronpa
sequence annotation: linker
sequence annotation: SV40 NLS
sequence annotation: Cre recombinase
sequence: https://synbiohub.org/public/igem/BBa_K1680021_sequence/1

Dronpa
type: SequenceAnnotation
persistent identity: annotation2477374
display id: annotation2477374
version: 1
top level: BBa_K1680021
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1680021/annotation2477374/range2477374/1

https://synbiohub.org/public/igem/BBa_K1680021/annotation2477374/range2477374/1
type: Range
persistent identity: range2477374
display id: range2477374
version: 1
direction: direction/forward
top level: BBa_K1680021
start: 31
end: 699
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation2477377
display id: annotation2477377
version: 1
top level: BBa_K1680021
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680021/annotation2477377/range2477377/1

https://synbiohub.org/public/igem/BBa_K1680021/annotation2477377/range2477377/1
type: Range
persistent identity: range2477377
display id: range2477377
version: 1
direction: direction/forward
top level: BBa_K1680021
start: 1747
end: 1764
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation2477375
display id: annotation2477375
version: 1
top level: BBa_K1680021
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680021/annotation2477375/range2477375/1

https://synbiohub.org/public/igem/BBa_K1680021/annotation2477375/range2477375/1
type: Range
persistent identity: range2477375
display id: range2477375
version: 1
direction: direction/forward
top level: BBa_K1680021
start: 700
end: 717
orientation: inline

SV40 NLS
type: SequenceAnnotation
persistent identity: annotation2477373
display id: annotation2477373
version: 1
top level: BBa_K1680021
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680021/annotation2477373/range2477373/1

https://synbiohub.org/public/igem/BBa_K1680021/annotation2477373/range2477373/1
type: Range
persistent identity: range2477373
display id: range2477373
version: 1
direction: direction/forward
top level: BBa_K1680021
start: 4
end: 24
orientation: inline

Dronpa
type: SequenceAnnotation
persistent identity: annotation2477378
display id: annotation2477378
version: 1
top level: BBa_K1680021
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1680021/annotation2477378/range2477378/1

https://synbiohub.org/public/igem/BBa_K1680021/annotation2477378/range2477378/1
type: Range
persistent identity: range2477378
display id: range2477378
version: 1
direction: direction/forward
top level: BBa_K1680021
start: 1765
end: 2433
orientation: inline

Cre recombinase
type: SequenceAnnotation
persistent identity: annotation2477376
display id: annotation2477376
version: 1
top level: BBa_K1680021
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1680021/annotation2477376/range2477376/1

https://synbiohub.org/public/igem/BBa_K1680021/annotation2477376/range2477376/1
type: Range
persistent identity: range2477376
display id: range2477376
version: 1
direction: direction/forward
top level: BBa_K1680021
start: 718
end: 1746
orientation: inline

BBa_K1680005
type: ComponentDefinition
persistent identity: BBa K1680005
display id: BBa_K1680005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: loxP Site
creator: Katharina Sporbeck
created: 2015-09-16T11:00:00Z
modified: 2015-10-28T06:30:39Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2098_
m user id: 26366
owner id: 26460
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part contains the WT loxP site, which is recognised by Cre recombinase (Part BBa_K1680007), in forward orientation.
mutable notes: Part is shipped in RFC10.
mutable provenance: gBlock designed by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K1680005_sequence/1

BBa_K112122
type: ComponentDefinition
persistent identity: BBa K112122
display id: BBa_K112122
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cre with stop codon
creator: Christina Brown
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:09:14Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 3241
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page] <!-- Add more about the biology of this part here
mutable notes: none
mutable provenance: cloned from E. coli MG1655 genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K112122_sequence/1

BBa_K1680008
type: ComponentDefinition
persistent identity: BBa K1680008
display id: BBa_K1680008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Δ1-19 Cre recombinase
creator: Katharina Sporbeck
created: 2015-09-16T11:00:00Z
modified: 2015-10-27T01:34:31Z
discontinued: false
dominant: false
group u list: _2098_
m user id: 15693
owner id: 26460
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding region for a mutant (Deletion of AA1-19) of DNA recombinase Cre, codon optimized for yeast.
mutable notes: To be used with RFC25
mutable provenance: gBlock, designed by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Delta1-19 Cre recombinase
sequence: https://synbiohub.org/public/igem/BBa_K1680008_sequence/1

Delta1-19 Cre recombinase
type: SequenceAnnotation
persistent identity: annotation2477412
display id: annotation2477412
version: 1
top level: BBa_K1680008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680008/annotation2477412/range2477412/1

https://synbiohub.org/public/igem/BBa_K1680008/annotation2477412/range2477412/1
type: Range
persistent identity: range2477412
display id: range2477412
version: 1
direction: direction/forward
top level: BBa_K1680008
start: 1
end: 972
orientation: inline

BBa_I718007
type: ComponentDefinition
persistent identity: BBa I718007
display id: BBa_I718007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS-CRE
creator: David Bikard
created: 2007-10-25T11:00:00Z
modified: 2015-08-31T04:07:52Z
discontinued: false
dominant: true
experience: experience/None
group u list: _141_
m user id: 0
owner id: 1481
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: none
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0030
component: BBa_J61047
sequence annotation: BBa_J61047
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_I718007_sequence/1

BBa_B0030
type: Component
persistent identity: component1956073
display id: component1956073
version: 1
top level: BBa_I718007
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_J61047
type: Component
persistent identity: component1956075
display id: component1956075
version: 1
top level: BBa_I718007
access: public
definition: Cre

BBa_J61047
type: SequenceAnnotation
persistent identity: annotation1956075
display id: annotation1956075
version: 1
top level: BBa_I718007
location: https://synbiohub.org/public/igem/BBa_I718007/annotation1956075/range1956075/1
component: BBa_J61047

https://synbiohub.org/public/igem/BBa_I718007/annotation1956075/range1956075/1
type: Range
persistent identity: range1956075
display id: range1956075
version: 1
direction: direction/reverse
top level: BBa_I718007
start: 22
end: 1058
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1956073
display id: annotation1956073
version: 1
top level: BBa_I718007
location: https://synbiohub.org/public/igem/BBa_I718007/annotation1956073/range1956073/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_I718007/annotation1956073/range1956073/1
type: Range
persistent identity: range1956073
display id: range1956073
version: 1
direction: direction/forward
top level: BBa_I718007
start: 1
end: 15
orientation: inline