//function/motility
type: Collection
persistent identity: function motility
display id: function_motility
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K283027
member: BBa_K518006
member: BBa_K777107
member: BBa_K283022
member: BBa_K777123
member: BBa_K108011
member: cheB
member: BBa_K108009
member: BBa_K777116
member: BBa_K094101
member: BBa_K108002
member: BBa_K777100
member: BBa_K518004
member: BBa_K283006
member: BBa_K777104
member: BBa_K777109
member: BBa_K283020
member: BBa_K777121
member: BBa_I712002
member: BBa_K1342001
member: BBa_K108005
member: BBa_K777120
member: BBa_K748007
member: BBa_K283003
member: BBa_K283016
member: BBa_K283033
member: BBa_K283021
member: BBa_K283034
member: BBa_K777114
member: BBa_K094103
member: cheZ
member: BBa_K819010
member: BBa_I752001
member: BBa_K108004
member: BBa_K1631019
member: BBa_K777102
member: BBa_K777115
member: BBa_K108008
member: BBa_K108003
member: BBa_I712017
member: BBa_K094100
member: BBa_K777110
member: BBa_J09271
member: BBa_K518005
member: BBa_K147000
member: BBa_K777101
member: BBa_K777103
member: BBa_K777108
member: BBa_K777122
member: aspA
member: BBa_K283023
member: BBa_K777113
member: BBa_K1342002
member: BBa_K108010
member: BBa_K1709003
member: BBa_K283019
member: BBa_K777117
member: BBa_K283002
member: BBa_K283008
member: BBa_K108006
member: BBa_K518008
member: BBa_K108031
member: BBa_K819016
member: BBa_K777118
member: BBa_K777105
member: BBa_K777112
member: BBa_K094106
member: BBa_K094110
member: BBa_K1631020
member: BBa_I712010
member: CheZ-GFP
member: BBa_K108007

BBa_K283027
type: ComponentDefinition
persistent identity: BBa K283027
display id: BBa_K283027
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rbs-GFP(LVA)-terminators (Backbone: pSB1AK3)
creator: Nelson Chu, W Ling , Shi Lei
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _383_
m user id: 0
owner id: 5494
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The chez is responsible for the dephosphorylation of cheY protein in bacteria flagella movement and is tagged by a 6-histidine tail. The pLac promoter can be repressed by LacI repressor protein
mutable notes: BBa_K283027
mutable provenance: The chez-histag coding region is self-designed The pLac promoter , rbs are from the registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0012
component: BBa_B0010
component: BBa_J04031
component: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_J04031
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_K283027_sequence/1

BBa_B0012
type: Component
persistent identity: component2056902
display id: component2056902
version: 1
top level: BBa_K283027
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component2056900
display id: component2056900
version: 1
top level: BBa_K283027
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_J04031
type: Component
persistent identity: component2056899
display id: component2056899
version: 1
top level: BBa_K283027
access: public
definition: https://synbiohub.org/public/igem/BBa_J04031/1

BBa_B0034
type: Component
persistent identity: component2056894
display id: component2056894
version: 1
top level: BBa_K283027
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2056902
display id: annotation2056902
version: 1
top level: BBa_K283027
location: https://synbiohub.org/public/igem/BBa_K283027/annotation2056902/range2056902/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K283027/annotation2056902/range2056902/1
type: Range
persistent identity: range2056902
display id: range2056902
version: 1
direction: direction/forward
top level: BBa_K283027
start: 874
end: 914
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2056894
display id: annotation2056894
version: 1
top level: BBa_K283027
location: https://synbiohub.org/public/igem/BBa_K283027/annotation2056894/range2056894/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K283027/annotation2056894/range2056894/1
type: Range
persistent identity: range2056894
display id: range2056894
version: 1
direction: direction/reverse
top level: BBa_K283027
start: 1
end: 12
orientation: inline

BBa_J04031
type: SequenceAnnotation
persistent identity: annotation2056899
display id: annotation2056899
version: 1
top level: BBa_K283027
location: https://synbiohub.org/public/igem/BBa_K283027/annotation2056899/range2056899/1
component: BBa_J04031

https://synbiohub.org/public/igem/BBa_K283027/annotation2056899/range2056899/1
type: Range
persistent identity: range2056899
display id: range2056899
version: 1
direction: direction/reverse
top level: BBa_K283027
start: 19
end: 777
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2056900
display id: annotation2056900
version: 1
top level: BBa_K283027
location: https://synbiohub.org/public/igem/BBa_K283027/annotation2056900/range2056900/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K283027/annotation2056900/range2056900/1
type: Range
persistent identity: range2056900
display id: range2056900
version: 1
direction: direction/forward
top level: BBa_K283027
start: 786
end: 865
orientation: inline

BBa_K518008
type: ComponentDefinition
persistent identity: BBa K518008
display id: BBa_K518008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IPTG-inducible CheZ repression device
creator: Masato Ohgishi
created: 2011-09-18T11:00:00Z
modified: 2015-05-08T01:12:33Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _683_
m user id: 0
owner id: 8305
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: CheZ is responsible for the dephosphorylation of flagellum-regulating protein CheY. It has been reported that cheZ-/- strain has a higher frequency of direction change and thus a narrower range of mobility. This device usually allows strong cheZ expression, while in the presense of IPTG, the transcription of cheZ is repressed by the overexpression of lambda cI repressor BBa_C0051.
mutable notes: None.
mutable provenance: cheZ CDS is cloned from Escherichia coli JM109 strain. Other sequences are from BioBrick parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_R0011
component: BBa_B0014
component: BBa_B0032
component: BBa_R0051
component: BBa_C0051
component: BBa_K518005
sequence annotation: BBa_C0051
sequence annotation: BBa_B0032
sequence annotation: BBa_R0011
sequence annotation: BBa_B0014
sequence annotation: BBa_K518005
sequence annotation: BBa_R0051
sequence: https://synbiohub.org/public/igem/BBa_K518008_sequence/1

BBa_R0011
type: Component
persistent identity: component2289746
display id: component2289746
version: 1
top level: BBa_K518008
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_B0014
type: Component
persistent identity: component2289745
display id: component2289745
version: 1
top level: BBa_K518008
access: public
definition: https://synbiohub.org/public/igem/BBa_B0014/1

BBa_B0014
type: Component
persistent identity: component2289764
display id: component2289764
version: 1
top level: BBa_K518008
access: public
definition: https://synbiohub.org/public/igem/BBa_B0014/1

BBa_B0032
type: Component
persistent identity: component2289736
display id: component2289736
version: 1
top level: BBa_K518008
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_R0051
type: Component
persistent identity: component2289731
display id: component2289731
version: 1
top level: BBa_K518008
access: public
definition: https://synbiohub.org/public/igem/BBa_R0051/1

BBa_C0051
type: Component
persistent identity: component2289757
display id: component2289757
version: 1
top level: BBa_K518008
access: public
definition: https://synbiohub.org/public/igem/BBa_C0051/1

BBa_B0032
type: Component
persistent identity: component2289752
display id: component2289752
version: 1
top level: BBa_K518008
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_K518005
type: Component
persistent identity: component2289738
display id: component2289738
version: 1
top level: BBa_K518008
access: public
definition: BBa_K518005

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation2289757
display id: annotation2289757
version: 1
top level: BBa_K518008
location: https://synbiohub.org/public/igem/BBa_K518008/annotation2289757/range2289757/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_K518008/annotation2289757/range2289757/1
type: Range
persistent identity: range2289757
display id: range2289757
version: 1
direction: direction/reverse
top level: BBa_K518008
start: 1077
end: 1851
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2289736
display id: annotation2289736
version: 1
top level: BBa_K518008
location: https://synbiohub.org/public/igem/BBa_K518008/annotation2289736/range2289736/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K518008/annotation2289736/range2289736/1
type: Range
persistent identity: range2289736
display id: range2289736
version: 1
direction: direction/forward
top level: BBa_K518008
start: 58
end: 70
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2289746
display id: annotation2289746
version: 1
top level: BBa_K518008
location: https://synbiohub.org/public/igem/BBa_K518008/annotation2289746/range2289746/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_K518008/annotation2289746/range2289746/1
type: Range
persistent identity: range2289746
display id: range2289746
version: 1
direction: direction/forward
top level: BBa_K518008
start: 995
end: 1048
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2289752
display id: annotation2289752
version: 1
top level: BBa_K518008
location: https://synbiohub.org/public/igem/BBa_K518008/annotation2289752/range2289752/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K518008/annotation2289752/range2289752/1
type: Range
persistent identity: range2289752
display id: range2289752
version: 1
direction: direction/forward
top level: BBa_K518008
start: 1058
end: 1070
orientation: inline

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2289764
display id: annotation2289764
version: 1
top level: BBa_K518008
location: https://synbiohub.org/public/igem/BBa_K518008/annotation2289764/range2289764/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K518008/annotation2289764/range2289764/1
type: Range
persistent identity: range2289764
display id: range2289764
version: 1
direction: direction/reverse
top level: BBa_K518008
start: 1860
end: 1954
orientation: inline

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2289745
display id: annotation2289745
version: 1
top level: BBa_K518008
location: https://synbiohub.org/public/igem/BBa_K518008/annotation2289745/range2289745/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K518008/annotation2289745/range2289745/1
type: Range
persistent identity: range2289745
display id: range2289745
version: 1
direction: direction/reverse
top level: BBa_K518008
start: 892
end: 986
orientation: inline

BBa_K518005
type: SequenceAnnotation
persistent identity: annotation2289738
display id: annotation2289738
version: 1
top level: BBa_K518008
location: https://synbiohub.org/public/igem/BBa_K518008/annotation2289738/range2289738/1
component: BBa_K518005

https://synbiohub.org/public/igem/BBa_K518008/annotation2289738/range2289738/1
type: Range
persistent identity: range2289738
display id: range2289738
version: 1
direction: direction/reverse
top level: BBa_K518008
start: 79
end: 883
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation2289731
display id: annotation2289731
version: 1
top level: BBa_K518008
location: https://synbiohub.org/public/igem/BBa_K518008/annotation2289731/range2289731/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_K518008/annotation2289731/range2289731/1
type: Range
persistent identity: range2289731
display id: range2289731
version: 1
direction: direction/forward
top level: BBa_K518008
start: 1
end: 49
orientation: inline

BBa_I752001
type: ComponentDefinition
persistent identity: BBa I752001
display id: BBa_I752001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CheZ coding sequence (Chemotaxis protein)
creator: David Franz
created: 2007-08-26T11:00:00Z
modified: 2016-10-08T03:28:13Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _129_
m user id: 25212
owner id: 1631
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: CheZ is one of the 6 Che proteins used in chemeotaxis. CheZ is a phosphatase which dephosphorylates CheY which in turn then allows the cell to run and tumble, wheres if CheZ is absence, CheY is phosphorylated, and the cell tumbles exclusively. (Topp and Gallivan J. Am. Chem. Soc. 129, p6865-6873)
mutable notes: blah
mutable provenance: blah
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: CheZ
sequence: https://synbiohub.org/public/igem/BBa_I752001_sequence/1

CheZ
type: SequenceAnnotation
persistent identity: annotation1944568
display id: annotation1944568
version: 1
top level: BBa_I752001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_I752001/annotation1944568/range1944568/1

https://synbiohub.org/public/igem/BBa_I752001/annotation1944568/range1944568/1
type: Range
persistent identity: range1944568
display id: range1944568
version: 1
direction: direction/forward
top level: BBa_I752001
start: 1
end: 639
orientation: inline

BBa_K518006
type: ComponentDefinition
persistent identity: BBa K518006
display id: BBa_K518006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IPTG-inducible CheZ expression device
creator: Masato Ohgishi
created: 2011-09-14T11:00:00Z
modified: 2015-05-08T01:12:33Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _683_
m user id: 0
owner id: 8305
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: CheZ is responsible for the dephosphorylation of flagellum-regulating protein CheY. It has been reported that cheZ-/- strain has a higher frequency of direction change and thus a narrower range of mobility. This device rescues cheZ-/- cells in the presence of IPTG.
mutable notes: None.
mutable provenance: cheZ CDS is cloned from Escherichia coli JM109 strain. Other sequences are from BioBrick parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K518005
component: BBa_B0032
component: BBa_R0011
component: BBa_B0014
sequence annotation: BBa_B0014
sequence annotation: BBa_K518005
sequence annotation: BBa_B0032
sequence annotation: BBa_R0011
sequence: https://synbiohub.org/public/igem/BBa_K518006_sequence/1

BBa_K518005
type: Component
persistent identity: component2128952
display id: component2128952
version: 1
top level: BBa_K518006
access: public
definition: BBa_K518005

BBa_B0032
type: Component
persistent identity: component2128950
display id: component2128950
version: 1
top level: BBa_K518006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_R0011
type: Component
persistent identity: component2128944
display id: component2128944
version: 1
top level: BBa_K518006
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_B0014
type: Component
persistent identity: component2128959
display id: component2128959
version: 1
top level: BBa_K518006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0014/1

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2128959
display id: annotation2128959
version: 1
top level: BBa_K518006
location: https://synbiohub.org/public/igem/BBa_K518006/annotation2128959/range2128959/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K518006/annotation2128959/range2128959/1
type: Range
persistent identity: range2128959
display id: range2128959
version: 1
direction: direction/reverse
top level: BBa_K518006
start: 898
end: 992
orientation: inline

BBa_K518005
type: SequenceAnnotation
persistent identity: annotation2128952
display id: annotation2128952
version: 1
top level: BBa_K518006
location: https://synbiohub.org/public/igem/BBa_K518006/annotation2128952/range2128952/1
component: BBa_K518005

https://synbiohub.org/public/igem/BBa_K518006/annotation2128952/range2128952/1
type: Range
persistent identity: range2128952
display id: range2128952
version: 1
direction: direction/reverse
top level: BBa_K518006
start: 85
end: 889
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2128950
display id: annotation2128950
version: 1
top level: BBa_K518006
location: https://synbiohub.org/public/igem/BBa_K518006/annotation2128950/range2128950/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K518006/annotation2128950/range2128950/1
type: Range
persistent identity: range2128950
display id: range2128950
version: 1
direction: direction/forward
top level: BBa_K518006
start: 64
end: 76
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2128944
display id: annotation2128944
version: 1
top level: BBa_K518006
location: https://synbiohub.org/public/igem/BBa_K518006/annotation2128944/range2128944/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_K518006/annotation2128944/range2128944/1
type: Range
persistent identity: range2128944
display id: range2128944
version: 1
direction: direction/forward
top level: BBa_K518006
start: 1
end: 54
orientation: inline

BBa_K283020
type: ComponentDefinition
persistent identity: BBa K283020
display id: BBa_K283020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FF_FF ansB promoter-rbs-cheZ_histag-terminators
creator: W Ling , Nelson Chu, Shi Lei
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _383_
m user id: 0
owner id: 5494
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part consist of a chez protein linked to a double terminator. The cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement. The chez coding region has a 6-Histidine tail and do not contain any rbs.
mutable notes: BBa_K283020
mutable provenance: The chez-histag coding region is self-designed The double terminator is from the registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_B0012
component: BBa_B0010
component: BBa_K283031
component: BBa_B0034
component: BBa_K283008
sequence annotation: BBa_K283008
sequence annotation: BBa_B0010
sequence annotation: BBa_K283031
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K283020_sequence/1

BBa_B0012
type: Component
persistent identity: component2056627
display id: component2056627
version: 1
top level: BBa_K283020
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component2056625
display id: component2056625
version: 1
top level: BBa_K283020
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_K283031
type: Component
persistent identity: component2056618
display id: component2056618
version: 1
top level: BBa_K283020
access: public
definition: https://synbiohub.org/public/igem/BBa_K283031/1

BBa_B0034
type: Component
persistent identity: component2056620
display id: component2056620
version: 1
top level: BBa_K283020
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K283008
type: Component
persistent identity: component2056624
display id: component2056624
version: 1
top level: BBa_K283020
access: public
definition: BBa_K283008

BBa_K283008
type: SequenceAnnotation
persistent identity: annotation2056624
display id: annotation2056624
version: 1
top level: BBa_K283020
location: https://synbiohub.org/public/igem/BBa_K283020/annotation2056624/range2056624/1
component: BBa_K283008

https://synbiohub.org/public/igem/BBa_K283020/annotation2056624/range2056624/1
type: Range
persistent identity: range2056624
display id: range2056624
version: 1
direction: direction/reverse
top level: BBa_K283020
start: 132
end: 844
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2056625
display id: annotation2056625
version: 1
top level: BBa_K283020
location: https://synbiohub.org/public/igem/BBa_K283020/annotation2056625/range2056625/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K283020/annotation2056625/range2056625/1
type: Range
persistent identity: range2056625
display id: range2056625
version: 1
direction: direction/forward
top level: BBa_K283020
start: 853
end: 932
orientation: inline

BBa_K283031
type: SequenceAnnotation
persistent identity: annotation2056618
display id: annotation2056618
version: 1
top level: BBa_K283020
location: https://synbiohub.org/public/igem/BBa_K283020/annotation2056618/range2056618/1
component: BBa_K283031

https://synbiohub.org/public/igem/BBa_K283020/annotation2056618/range2056618/1
type: Range
persistent identity: range2056618
display id: range2056618
version: 1
direction: direction/reverse
top level: BBa_K283020
start: 1
end: 105
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2056620
display id: annotation2056620
version: 1
top level: BBa_K283020
location: https://synbiohub.org/public/igem/BBa_K283020/annotation2056620/range2056620/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K283020/annotation2056620/range2056620/1
type: Range
persistent identity: range2056620
display id: range2056620
version: 1
direction: direction/reverse
top level: BBa_K283020
start: 114
end: 125
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2056627
display id: annotation2056627
version: 1
top level: BBa_K283020
location: https://synbiohub.org/public/igem/BBa_K283020/annotation2056627/range2056627/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K283020/annotation2056627/range2056627/1
type: Range
persistent identity: range2056627
display id: range2056627
version: 1
direction: direction/forward
top level: BBa_K283020
start: 941
end: 981
orientation: inline

BBa_K283006
type: ComponentDefinition
persistent identity: BBa K283006
display id: BBa_K283006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cheZ-his tag-double terminators
creator: Nelson Chu, W Ling , Shi Lei
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _383_
m user id: 0
owner id: 5494
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The CI repressor protein is constitutively expressed under the pLux promoter. The CI protein represses the transcription of plambda(R-O12). This part lacks a terminator
mutable notes: BBa_K283006
mutable provenance: The CI, terminator, pLambda promoter and the chez gene are solf designed the pLux promoter, rbs are from the registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0012
component: BBa_B0010
component: BBa_K283008
sequence annotation: BBa_K283008
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K283006_sequence/1

BBa_B0012
type: Component
persistent identity: component2055644
display id: component2055644
version: 1
top level: BBa_K283006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component2055642
display id: component2055642
version: 1
top level: BBa_K283006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_K283008
type: Component
persistent identity: component2055641
display id: component2055641
version: 1
top level: BBa_K283006
access: public
definition: BBa_K283008

BBa_K283008
type: SequenceAnnotation
persistent identity: annotation2055641
display id: annotation2055641
version: 1
top level: BBa_K283006
location: https://synbiohub.org/public/igem/BBa_K283006/annotation2055641/range2055641/1
component: BBa_K283008

https://synbiohub.org/public/igem/BBa_K283006/annotation2055641/range2055641/1
type: Range
persistent identity: range2055641
display id: range2055641
version: 1
direction: direction/reverse
top level: BBa_K283006
start: 1
end: 713
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2055642
display id: annotation2055642
version: 1
top level: BBa_K283006
location: https://synbiohub.org/public/igem/BBa_K283006/annotation2055642/range2055642/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K283006/annotation2055642/range2055642/1
type: Range
persistent identity: range2055642
display id: range2055642
version: 1
direction: direction/forward
top level: BBa_K283006
start: 722
end: 801
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2055644
display id: annotation2055644
version: 1
top level: BBa_K283006
location: https://synbiohub.org/public/igem/BBa_K283006/annotation2055644/range2055644/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K283006/annotation2055644/range2055644/1
type: Range
persistent identity: range2055644
display id: range2055644
version: 1
direction: direction/forward
top level: BBa_K283006
start: 810
end: 850
orientation: inline

BBa_K108002
type: ComponentDefinition
persistent identity: BBa K108002
display id: BBa_K108002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FlgA
creator: Qi Liu
created: 2008-10-14T11:00:00Z
modified: 2015-05-08T01:09:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _222_
m user id: 0
owner id: 2671
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: assembly protein for flagellar basal-body periplasmic P ring
mutable notes: PCR using primers with biobrick prefix and surfix, and then cloned into pSB1AC3.
mutable provenance: E.coli K12 DH10B genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: FlgA
sequence: https://synbiohub.org/public/igem/BBa_K108002_sequence/1

FlgA
type: SequenceAnnotation
persistent identity: annotation1981496
display id: annotation1981496
version: 1
top level: BBa_K108002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K108002/annotation1981496/range1981496/1

https://synbiohub.org/public/igem/BBa_K108002/annotation1981496/range1981496/1
type: Range
persistent identity: range1981496
display id: range1981496
version: 1
direction: direction/forward
top level: BBa_K108002
start: 1
end: 657
orientation: inline

BBa_K777119
type: ComponentDefinition
persistent identity: BBa K777119
display id: BBa_K777119
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: motB under the control of constitutive promoter J23112
creator: Team Goettingen
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This gene codes for MotB, a part of the flagellar motor. MotA and MotB constitute the stator and are required for the flow of ions and torque generation. Here we used 3 different constitutive [http://partsregistry.org/Part:BBa_J23100 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''motB'' expression affect the motility of ''E. coli''.
mutable notes: * ''motB'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: ** Fwd: 5'-gtttcttcgaattcgcggccgcttctagatgaagaatcaagcgcatcc-3' ** Rev: 5'-gtttcttcctgcagcggccgctactagtatcacctcggttcggctgatgg-3'
mutable provenance: * The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). * J23112 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K777117
component: BBa_J23112
sequence annotation: BBa_K777117
sequence annotation: BBa_J23112
sequence: https://synbiohub.org/public/igem/BBa_K777119_sequence/1

BBa_K777117
type: Component
persistent identity: component2190798
display id: component2190798
version: 1
top level: BBa_K777119
access: public
definition: BBa_K777117

BBa_J23112
type: Component
persistent identity: component2190796
display id: component2190796
version: 1
top level: BBa_K777119
access: public
definition: https://synbiohub.org/public/igem/BBa_J23112/1

BBa_K777117
type: SequenceAnnotation
persistent identity: annotation2190798
display id: annotation2190798
version: 1
top level: BBa_K777119
location: https://synbiohub.org/public/igem/BBa_K777119/annotation2190798/range2190798/1
component: BBa_K777117

https://synbiohub.org/public/igem/BBa_K777119/annotation2190798/range2190798/1
type: Range
persistent identity: range2190798
display id: range2190798
version: 1
direction: direction/reverse
top level: BBa_K777119
start: 42
end: 968
orientation: inline

BBa_J23112
type: SequenceAnnotation
persistent identity: annotation2190796
display id: annotation2190796
version: 1
top level: BBa_K777119
location: https://synbiohub.org/public/igem/BBa_K777119/annotation2190796/range2190796/1
component: BBa_J23112

https://synbiohub.org/public/igem/BBa_K777119/annotation2190796/range2190796/1
type: Range
persistent identity: range2190796
display id: range2190796
version: 1
direction: direction/reverse
top level: BBa_K777119
start: 1
end: 35
orientation: inline

BBa_K518004
type: ComponentDefinition
persistent identity: BBa K518004
display id: BBa_K518004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IPTG-inducible L-Asp producing device
creator: Masato Ohgishi
created: 2011-09-14T11:00:00Z
modified: 2015-05-08T01:12:33Z
discontinued: false
dominant: false
group u list: _683_
m user id: 0
owner id: 8305
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: AspA is an aspartate ammonia-lyase, which produces aspartate from fumarate and ammonium ion. Aspartate can be used as a chemoattractant for bacteria including Escherichia coli. In this device, AspA CDS, flanked with RBS and terminator, is under control of lacI-regulated strong promoter. IPTG can be used to induce aspartate production.
mutable notes: None.
mutable provenance: From BioBrick parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0032
component: BBa_R0011
component: BBa_C0083
component: BBa_B0014
sequence annotation: BBa_B0014
sequence annotation: BBa_R0011
sequence annotation: BBa_C0083
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_K518004_sequence/1

BBa_B0032
type: Component
persistent identity: component2258713
display id: component2258713
version: 1
top level: BBa_K518004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_R0011
type: Component
persistent identity: component2258707
display id: component2258707
version: 1
top level: BBa_K518004
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_C0083
type: Component
persistent identity: component2258723
display id: component2258723
version: 1
top level: BBa_K518004
access: public
definition: aspA

BBa_B0014
type: Component
persistent identity: component2258730
display id: component2258730
version: 1
top level: BBa_K518004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0014/1

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2258730
display id: annotation2258730
version: 1
top level: BBa_K518004
location: https://synbiohub.org/public/igem/BBa_K518004/annotation2258730/range2258730/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K518004/annotation2258730/range2258730/1
type: Range
persistent identity: range2258730
display id: range2258730
version: 1
direction: direction/reverse
top level: BBa_K518004
start: 1634
end: 1728
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2258707
display id: annotation2258707
version: 1
top level: BBa_K518004
location: https://synbiohub.org/public/igem/BBa_K518004/annotation2258707/range2258707/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_K518004/annotation2258707/range2258707/1
type: Range
persistent identity: range2258707
display id: range2258707
version: 1
direction: direction/forward
top level: BBa_K518004
start: 1
end: 54
orientation: inline

BBa_C0083
type: SequenceAnnotation
persistent identity: annotation2258723
display id: annotation2258723
version: 1
top level: BBa_K518004
location: https://synbiohub.org/public/igem/BBa_K518004/annotation2258723/range2258723/1
component: BBa_C0083

https://synbiohub.org/public/igem/BBa_K518004/annotation2258723/range2258723/1
type: Range
persistent identity: range2258723
display id: range2258723
version: 1
direction: direction/reverse
top level: BBa_K518004
start: 83
end: 1625
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2258713
display id: annotation2258713
version: 1
top level: BBa_K518004
location: https://synbiohub.org/public/igem/BBa_K518004/annotation2258713/range2258713/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K518004/annotation2258713/range2258713/1
type: Range
persistent identity: range2258713
display id: range2258713
version: 1
direction: direction/forward
top level: BBa_K518004
start: 64
end: 76
orientation: inline

BBa_J09271
type: ComponentDefinition
persistent identity: BBa J09271
display id: BBa_J09271
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: motB flagellar protein, without endogenous RBS
creator: Jon Badalamenti
created: 2006-10-25T11:00:00Z
modified: 2015-08-31T04:08:21Z
discontinued: false
dominant: true
experience: experience/None
group u list: _72_40_
m user id: 0
owner id: 475
owning group id: 72
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: motB E. coli flagellar protein linking torque machinery to cell wall; allows for induction of motility
mutable notes: We had to design this part to address leaky expression from R0062. In order to induce motility with HSL, we must be able to demonstrate both repression and induction. S03271 contains the endogenous RBS of motB and is thought to be strong. Leaky expression could be diminished by adding weaker ribosome binding sites upstream of this part.
mutable provenance: This part was amplified by PCR from the genome of E. coli strain RP437 (wild-type for motility)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_J09271_sequence/1

BBa_K283033
type: ComponentDefinition
persistent identity: BBa K283033
display id: BBa_K283033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter-rbs-CI (lambda LVA)
creator: Shi Lei, Nelson Chu, W Ling
created: 2009-10-15T11:00:00Z
modified: 2015-05-08T01:11:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _383_
m user id: 0
owner id: 5494
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part generates chez protein under a hypoxia inducible promoter panas. The cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement and is tagged by a 6-Histidine tail.
mutable notes: BBa_K283033
mutable provenance: The rbs, double terminator comes from the registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_C0051
component: BBa_B0034
component: BBa_J23100
sequence annotation: BBa_J23100
sequence annotation: BBa_B0034
sequence annotation: BBa_C0051
sequence: https://synbiohub.org/public/igem/BBa_K283033_sequence/1

BBa_C0051
type: Component
persistent identity: component2246773
display id: component2246773
version: 1
top level: BBa_K283033
access: public
definition: https://synbiohub.org/public/igem/BBa_C0051/1

BBa_B0034
type: Component
persistent identity: component2246769
display id: component2246769
version: 1
top level: BBa_K283033
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_J23100
type: Component
persistent identity: component2246767
display id: component2246767
version: 1
top level: BBa_K283033
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2246767
display id: annotation2246767
version: 1
top level: BBa_K283033
location: https://synbiohub.org/public/igem/BBa_K283033/annotation2246767/range2246767/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K283033/annotation2246767/range2246767/1
type: Range
persistent identity: range2246767
display id: range2246767
version: 1
direction: direction/reverse
top level: BBa_K283033
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2246769
display id: annotation2246769
version: 1
top level: BBa_K283033
location: https://synbiohub.org/public/igem/BBa_K283033/annotation2246769/range2246769/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K283033/annotation2246769/range2246769/1
type: Range
persistent identity: range2246769
display id: range2246769
version: 1
direction: direction/reverse
top level: BBa_K283033
start: 44
end: 55
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation2246773
display id: annotation2246773
version: 1
top level: BBa_K283033
location: https://synbiohub.org/public/igem/BBa_K283033/annotation2246773/range2246773/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_K283033/annotation2246773/range2246773/1
type: Range
persistent identity: range2246773
display id: range2246773
version: 1
direction: direction/reverse
top level: BBa_K283033
start: 62
end: 836
orientation: inline

BBa_K777113
type: ComponentDefinition
persistent identity: BBa K777113
display id: BBa_K777113
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: motA
creator: Team Goettingen
created: 2012-09-16T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The motA gene codes for a part of the flagellar motor complex. MotA proteins represent the proton conducting component of this molecular machine. Thus it is necessary for flagellar rotation.
mutable notes: *''motA'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: **Fwd: GTTTCTTCGAATTCGCGGCCGCTTCTAGATGACCGGTGGAAGCCTTGG **Rev: GTTTCTTCCTGCAGCGGCCGCTACTAGTATCATGCTTCCTCGGTTGTCG
mutable provenance: * The part was amplified from genomic DNA of E. coli str. K-12 substr. DH10B, complete genome (CP000948.1).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: stop
sequence annotation: MotA coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K777113_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2184143
display id: annotation2184143
version: 1
top level: BBa_K777113
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K777113/annotation2184143/range2184143/1

https://synbiohub.org/public/igem/BBa_K777113/annotation2184143/range2184143/1
type: Range
persistent identity: range2184143
display id: range2184143
version: 1
direction: direction/forward
top level: BBa_K777113
start: 1
end: 1
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2184144
display id: annotation2184144
version: 1
top level: BBa_K777113
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K777113/annotation2184144/range2184144/1

https://synbiohub.org/public/igem/BBa_K777113/annotation2184144/range2184144/1
type: Range
persistent identity: range2184144
display id: range2184144
version: 1
direction: direction/forward
top level: BBa_K777113
start: 831
end: 831
orientation: inline

MotA coding sequence
type: SequenceAnnotation
persistent identity: annotation2184142
display id: annotation2184142
version: 1
top level: BBa_K777113
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K777113/annotation2184142/range2184142/1

https://synbiohub.org/public/igem/BBa_K777113/annotation2184142/range2184142/1
type: Range
persistent identity: range2184142
display id: range2184142
version: 1
direction: direction/forward
top level: BBa_K777113
start: 1
end: 831
orientation: inline

BBa_K777108
type: ComponentDefinition
persistent identity: BBa K777108
display id: BBa_K777108
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: flhDC operon under the control of constitutive promoter J23114
creator: Team Goettingen
created: 2012-09-18T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The ''flhDC'' operon is the master regulator of motility and chemotaxis in ''E. coli''. FlhDC forms heterotetramers and activates class II operons in concert with sigma factor 70. Among the gene products of class II operons are several components of the flagellum and the alternative sigma factor FliA which is essential for the transcription of class III genes. Here we used a set of 8 constitutive Anderson-promoters from the 2006 Berkeley group to test how different levels of constitutive ''flhDC'' expression affect the motility of ''E. coli''.
mutable notes: *The PstI restriction site in the ''flhDC'' sequence was mutated to CTGCGG using the reverse primer. *''flhDC'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: **Fwd: 5'-actgaattcgcggccgcttctagatgcatacctccgagttgctg-3' **Rev: 5'-tcctgcagcggccgctactagttactgcccgggatggcggttgacataagcCgcaggcaaagctgccaacag-3' (the capitalized "C" induces the mutation for removal of the PstI site)
mutable provenance: *The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). *J23100 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K777100
component: BBa_J23114
sequence annotation: BBa_J23114
sequence annotation: BBa_K777100
sequence: https://synbiohub.org/public/igem/BBa_K777108_sequence/1

BBa_K777100
type: Component
persistent identity: component2187126
display id: component2187126
version: 1
top level: BBa_K777108
access: public
definition: BBa_K777100

BBa_J23114
type: Component
persistent identity: component2187118
display id: component2187118
version: 1
top level: BBa_K777108
access: public
definition: https://synbiohub.org/public/igem/BBa_J23114/1

BBa_J23114
type: SequenceAnnotation
persistent identity: annotation2187118
display id: annotation2187118
version: 1
top level: BBa_K777108
location: https://synbiohub.org/public/igem/BBa_K777108/annotation2187118/range2187118/1
component: BBa_J23114

https://synbiohub.org/public/igem/BBa_K777108/annotation2187118/range2187118/1
type: Range
persistent identity: range2187118
display id: range2187118
version: 1
direction: direction/reverse
top level: BBa_K777108
start: 1
end: 35
orientation: inline

BBa_K777100
type: SequenceAnnotation
persistent identity: annotation2187126
display id: annotation2187126
version: 1
top level: BBa_K777108
location: https://synbiohub.org/public/igem/BBa_K777108/annotation2187126/range2187126/1
component: BBa_K777100

https://synbiohub.org/public/igem/BBa_K777108/annotation2187126/range2187126/1
type: Range
persistent identity: range2187126
display id: range2187126
version: 1
direction: direction/reverse
top level: BBa_K777108
start: 42
end: 910
orientation: inline

BBa_K748007
type: ComponentDefinition
persistent identity: BBa K748007
display id: BBa_K748007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Biofilm formation device. yddV gene with the IPTG inducible promoter.
creator: Lei Qiao
created: 2012-09-14T11:00:00Z
modified: 2015-05-08T01:13:12Z
discontinued: false
dominant: false
experience: experience/None
group u list: _999_
m user id: 0
owner id: 8564
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Gene yddV is under the regulation of Ptrc promoter (IPTG-inducible). yddV is a Diguanylate Cyclase-Genomic. The product of gene yddV has diguanylate cyclase (DGC) activity. DGC uses 2 GTP to form a Bis-(3???-5???)-cyclic dimeric guanosine monophosphate (c-di-GMP). C-di-GMP is a global second messenger in bacteria. Biofilm formation of E.coli is manipulable by varying c-di-GMP concentrations. When the c-di-GMP level stays low, there is little biofilm and the bacteria is dispersive. High concentrations of c-di-GMP promote bacteria to form more cellulose and fimbriae, which enhances the biofilm formation and decrease the motility of bacteria.
mutable notes: nothing special
mutable provenance: de novo synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0011
component: BBa_B0034
component: BBa_K748003
component: BBa_B0015
sequence annotation: BBa_B0015
sequence annotation: BBa_R0011
sequence annotation: BBa_B0034
sequence annotation: BBa_K748003
sequence: https://synbiohub.org/public/igem/BBa_K748007_sequence/1

BBa_R0011
type: Component
persistent identity: component2183395
display id: component2183395
version: 1
top level: BBa_K748007
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_B0034
type: Component
persistent identity: component2183401
display id: component2183401
version: 1
top level: BBa_K748007
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K748003
type: Component
persistent identity: component2183402
display id: component2183402
version: 1
top level: BBa_K748007
access: public
definition: https://synbiohub.org/public/igem/BBa_K748003/1

BBa_B0015
type: Component
persistent identity: component2183409
display id: component2183409
version: 1
top level: BBa_K748007
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2183409
display id: annotation2183409
version: 1
top level: BBa_K748007
location: https://synbiohub.org/public/igem/BBa_K748007/annotation2183409/range2183409/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K748007/annotation2183409/range2183409/1
type: Range
persistent identity: range2183409
display id: range2183409
version: 1
direction: direction/reverse
top level: BBa_K748007
start: 1476
end: 1604
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2183395
display id: annotation2183395
version: 1
top level: BBa_K748007
location: https://synbiohub.org/public/igem/BBa_K748007/annotation2183395/range2183395/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_K748007/annotation2183395/range2183395/1
type: Range
persistent identity: range2183395
display id: range2183395
version: 1
direction: direction/forward
top level: BBa_K748007
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2183401
display id: annotation2183401
version: 1
top level: BBa_K748007
location: https://synbiohub.org/public/igem/BBa_K748007/annotation2183401/range2183401/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K748007/annotation2183401/range2183401/1
type: Range
persistent identity: range2183401
display id: range2183401
version: 1
direction: direction/reverse
top level: BBa_K748007
start: 64
end: 75
orientation: inline

BBa_K748003
type: SequenceAnnotation
persistent identity: annotation2183402
display id: annotation2183402
version: 1
top level: BBa_K748007
location: https://synbiohub.org/public/igem/BBa_K748007/annotation2183402/range2183402/1
component: BBa_K748003

https://synbiohub.org/public/igem/BBa_K748007/annotation2183402/range2183402/1
type: Range
persistent identity: range2183402
display id: range2183402
version: 1
direction: direction/reverse
top level: BBa_K748007
start: 82
end: 1467
orientation: inline

BBa_K108008
type: ComponentDefinition
persistent identity: BBa K108008
display id: BBa_K108008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FliI
creator: Qi Liu
created: 2008-10-22T11:00:00Z
modified: 2015-05-08T01:09:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _222_
m user id: 0
owner id: 2671
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: flagellum-specific ATP synthase
mutable notes: PCR using primers with biobrick prefix and surfix, and then cloned into pSB1AC3.
mutable provenance: E.coli K12 DH10B genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: FliI
sequence: https://synbiohub.org/public/igem/BBa_K108008_sequence/1

FliI
type: SequenceAnnotation
persistent identity: annotation1986953
display id: annotation1986953
version: 1
top level: BBa_K108008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K108008/annotation1986953/range1986953/1

https://synbiohub.org/public/igem/BBa_K108008/annotation1986953/range1986953/1
type: Range
persistent identity: range1986953
display id: range1986953
version: 1
direction: direction/forward
top level: BBa_K108008
start: 1
end: 1371
orientation: inline

BBa_K777117
type: ComponentDefinition
persistent identity: BBa K777117
display id: BBa_K777117
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: motB
creator: Team Goettingen
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This gene codes for MotB, a part of the flagellar motor. MotA and MotB constitute the stator and are required for the flow of ions and torque generation.
mutable notes: * motA was amplified from genomic DNA of E. coli DH10B via PCR using the following primers: ** Fwd: GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAAGAATCAAGCGCATCC ** Rev: GTTTCTTCCTGCAGCGGCCGCTACTAGTATCACCTCGGTTCGGCTGATGG
mutable provenance: * The part was amplified from genomic DNA of E. coli str. K-12 substr. DH10B, complete genome (CP000948.1).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: MotB coding region
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K777117_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2184527
display id: annotation2184527
version: 1
top level: BBa_K777117
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K777117/annotation2184527/range2184527/1

https://synbiohub.org/public/igem/BBa_K777117/annotation2184527/range2184527/1
type: Range
persistent identity: range2184527
display id: range2184527
version: 1
direction: direction/forward
top level: BBa_K777117
start: 927
end: 927
orientation: inline

MotB coding region
type: SequenceAnnotation
persistent identity: annotation2184525
display id: annotation2184525
version: 1
top level: BBa_K777117
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K777117/annotation2184525/range2184525/1

https://synbiohub.org/public/igem/BBa_K777117/annotation2184525/range2184525/1
type: Range
persistent identity: range2184525
display id: range2184525
version: 1
direction: direction/forward
top level: BBa_K777117
start: 1
end: 927
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2184526
display id: annotation2184526
version: 1
top level: BBa_K777117
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K777117/annotation2184526/range2184526/1

https://synbiohub.org/public/igem/BBa_K777117/annotation2184526/range2184526/1
type: Range
persistent identity: range2184526
display id: range2184526
version: 1
direction: direction/forward
top level: BBa_K777117
start: 1
end: 1
orientation: inline

BBa_K777110
type: ComponentDefinition
persistent identity: BBa K777110
display id: BBa_K777110
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fliC under the control of constitutive promoter J23100
creator: Team Goettingen
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The ''fliC'' gene codes for flagellin. This protein is the subunit that forms the flagellar filament of ''E. coli''. Here we used 3 different constitutive [http://partsregistry.org/Part:BBa_J23100 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''fliC'' expression affect the motility or flagellum size of ''E. coli''.
mutable notes: * The ''fliC'' gene contained three PstI sites and one SpeI site that had to be removed. These mutations were induced with different primers via overlap-PCR. * ''fliC'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: ** Fwd: 5'-gtttcttcgaattcgcggccgcttctagatggcacaagtcattaatacc-3' ** Rev: 5'-gtttcttcgaattcgcggccgcttctagttaaccctgGagcagagacagaacc-3' (the capitalized "G" induces the mutation for removal of the third PstI site)
mutable provenance: * The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K777109
component: BBa_J23100
sequence annotation: BBa_J23100
sequence annotation: BBa_K777109
sequence: https://synbiohub.org/public/igem/BBa_K777110_sequence/1

BBa_K777109
type: Component
persistent identity: component2189311
display id: component2189311
version: 1
top level: BBa_K777110
access: public
definition: BBa_K777109

BBa_J23100
type: Component
persistent identity: component2189303
display id: component2189303
version: 1
top level: BBa_K777110
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2189303
display id: annotation2189303
version: 1
top level: BBa_K777110
location: https://synbiohub.org/public/igem/BBa_K777110/annotation2189303/range2189303/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K777110/annotation2189303/range2189303/1
type: Range
persistent identity: range2189303
display id: range2189303
version: 1
direction: direction/reverse
top level: BBa_K777110
start: 1
end: 35
orientation: inline

BBa_K777109
type: SequenceAnnotation
persistent identity: annotation2189311
display id: annotation2189311
version: 1
top level: BBa_K777110
location: https://synbiohub.org/public/igem/BBa_K777110/annotation2189311/range2189311/1
component: BBa_K777109

https://synbiohub.org/public/igem/BBa_K777110/annotation2189311/range2189311/1
type: Range
persistent identity: range2189311
display id: range2189311
version: 1
direction: direction/reverse
top level: BBa_K777110
start: 42
end: 1538
orientation: inline

BBa_K777112
type: ComponentDefinition
persistent identity: BBa K777112
display id: BBa_K777112
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fliC under the control of constitutive promoter J23114
creator: Team Goettingen
created: 2012-09-21T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The ''fliC'' gene codes for flagellin. This protein is the subunit that forms the flagellar filament of ''E. coli''. Here we used 3 different constitutive [http://partsregistry.org/Part:BBa_J23100 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''fliC'' expression affect the motility or flagellum size of ''E. coli''.
mutable notes: * The ''fliC'' gene contained three PstI sites and one SpeI site that had to be removed. These mutations were induced with different primers via overlap-PCR.
mutable provenance: * The ''fliC'' gene was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). * J23100 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K777109
component: BBa_J23114
sequence annotation: BBa_J23114
sequence annotation: BBa_K777109
sequence: https://synbiohub.org/public/igem/BBa_K777112_sequence/1

BBa_K777109
type: Component
persistent identity: component2192754
display id: component2192754
version: 1
top level: BBa_K777112
access: public
definition: BBa_K777109

BBa_J23114
type: Component
persistent identity: component2192746
display id: component2192746
version: 1
top level: BBa_K777112
access: public
definition: https://synbiohub.org/public/igem/BBa_J23114/1

BBa_J23114
type: SequenceAnnotation
persistent identity: annotation2192746
display id: annotation2192746
version: 1
top level: BBa_K777112
location: https://synbiohub.org/public/igem/BBa_K777112/annotation2192746/range2192746/1
component: BBa_J23114

https://synbiohub.org/public/igem/BBa_K777112/annotation2192746/range2192746/1
type: Range
persistent identity: range2192746
display id: range2192746
version: 1
direction: direction/reverse
top level: BBa_K777112
start: 1
end: 35
orientation: inline

BBa_K777109
type: SequenceAnnotation
persistent identity: annotation2192754
display id: annotation2192754
version: 1
top level: BBa_K777112
location: https://synbiohub.org/public/igem/BBa_K777112/annotation2192754/range2192754/1
component: BBa_K777109

https://synbiohub.org/public/igem/BBa_K777112/annotation2192754/range2192754/1
type: Range
persistent identity: range2192754
display id: range2192754
version: 1
direction: direction/reverse
top level: BBa_K777112
start: 42
end: 1538
orientation: inline

BBa_K108009
type: ComponentDefinition
persistent identity: BBa K108009
display id: BBa_K108009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FliK
creator: Qi Liu
created: 2008-10-22T11:00:00Z
modified: 2015-05-08T01:09:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _222_
m user id: 0
owner id: 2671
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: flagellar hook-length control protein
mutable notes: PCR using primers with biobrick prefix and surfix, and then cloned into pSB1AC3.
mutable provenance: E.coli K12 DH10B genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: FliK
sequence: https://synbiohub.org/public/igem/BBa_K108009_sequence/1

FliK
type: SequenceAnnotation
persistent identity: annotation1986954
display id: annotation1986954
version: 1
top level: BBa_K108009
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K108009/annotation1986954/range1986954/1

https://synbiohub.org/public/igem/BBa_K108009/annotation1986954/range1986954/1
type: Range
persistent identity: range1986954
display id: range1986954
version: 1
direction: direction/forward
top level: BBa_K108009
start: 1
end: 1125
orientation: inline

BBa_K283002
type: ComponentDefinition
persistent identity: BBa K283002
display id: BBa_K283002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Speed control device (pLacI-rbs-TetR-terminator-ptet-rbs-chez_his-double terminators)
creator: Shi Lei, Nelson Chu, W Ling
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _383_
m user id: 0
owner id: 5494
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This is a device that enable the control of chez(histag) using IPTG. The pLacIq promoter enables high level of expression of the LacI repressor protein. The LacI protein represses the Plac promoter. IPTG binds with LacI repressor protein and remove the inhibiting effect of the LacI on the pLac promoter.
mutable notes: BBa_K283002
mutable provenance: The rbs, terminator and the pLac promoter are from the registry, the rest of the part are self designed
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_R0010
component: BBa_B0012
component: BBa_R0040
component: BBa_K283008
component: BBa_B0034
component: BBa_C0040
component: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_R0040
sequence annotation: BBa_B0010
sequence annotation: BBa_K283008
sequence annotation: BBa_R0010
sequence annotation: BBa_C0040
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K283002_sequence/1

BBa_R0010
type: Component
persistent identity: component2287603
display id: component2287603
version: 1
top level: BBa_K283002
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0012
type: Component
persistent identity: component2287618
display id: component2287618
version: 1
top level: BBa_K283002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
type: Component
persistent identity: component2287635
display id: component2287635
version: 1
top level: BBa_K283002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0040
type: Component
persistent identity: component2287622
display id: component2287622
version: 1
top level: BBa_K283002
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_K283008
type: Component
persistent identity: component2287632
display id: component2287632
version: 1
top level: BBa_K283002
access: public
definition: BBa_K283008

BBa_B0034
type: Component
persistent identity: component2287628
display id: component2287628
version: 1
top level: BBa_K283002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0040
type: Component
persistent identity: component2287615
display id: component2287615
version: 1
top level: BBa_K283002
access: public
definition: https://synbiohub.org/public/igem/BBa_C0040/1

BBa_B0010
type: Component
persistent identity: component2287616
display id: component2287616
version: 1
top level: BBa_K283002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component2287611
display id: component2287611
version: 1
top level: BBa_K283002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component2287633
display id: component2287633
version: 1
top level: BBa_K283002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2287628
display id: annotation2287628
version: 1
top level: BBa_K283002
location: https://synbiohub.org/public/igem/BBa_K283002/annotation2287628/range2287628/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K283002/annotation2287628/range2287628/1
type: Range
persistent identity: range2287628
display id: range2287628
version: 1
direction: direction/reverse
top level: BBa_K283002
start: 1119
end: 1130
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2287622
display id: annotation2287622
version: 1
top level: BBa_K283002
location: https://synbiohub.org/public/igem/BBa_K283002/annotation2287622/range2287622/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K283002/annotation2287622/range2287622/1
type: Range
persistent identity: range2287622
display id: range2287622
version: 1
direction: direction/forward
top level: BBa_K283002
start: 1057
end: 1110
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2287633
display id: annotation2287633
version: 1
top level: BBa_K283002
location: https://synbiohub.org/public/igem/BBa_K283002/annotation2287633/range2287633/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K283002/annotation2287633/range2287633/1
type: Range
persistent identity: range2287633
display id: range2287633
version: 1
direction: direction/forward
top level: BBa_K283002
start: 1858
end: 1937
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2287616
display id: annotation2287616
version: 1
top level: BBa_K283002
location: https://synbiohub.org/public/igem/BBa_K283002/annotation2287616/range2287616/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K283002/annotation2287616/range2287616/1
type: Range
persistent identity: range2287616
display id: range2287616
version: 1
direction: direction/forward
top level: BBa_K283002
start: 920
end: 999
orientation: inline

BBa_K283008
type: SequenceAnnotation
persistent identity: annotation2287632
display id: annotation2287632
version: 1
top level: BBa_K283002
location: https://synbiohub.org/public/igem/BBa_K283002/annotation2287632/range2287632/1
component: BBa_K283008

https://synbiohub.org/public/igem/BBa_K283002/annotation2287632/range2287632/1
type: Range
persistent identity: range2287632
display id: range2287632
version: 1
direction: direction/reverse
top level: BBa_K283002
start: 1137
end: 1849
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2287603
display id: annotation2287603
version: 1
top level: BBa_K283002
location: https://synbiohub.org/public/igem/BBa_K283002/annotation2287603/range2287603/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K283002/annotation2287603/range2287603/1
type: Range
persistent identity: range2287603
display id: range2287603
version: 1
direction: direction/forward
top level: BBa_K283002
start: 1
end: 200
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2287615
display id: annotation2287615
version: 1
top level: BBa_K283002
location: https://synbiohub.org/public/igem/BBa_K283002/annotation2287615/range2287615/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K283002/annotation2287615/range2287615/1
type: Range
persistent identity: range2287615
display id: range2287615
version: 1
direction: direction/reverse
top level: BBa_K283002
start: 227
end: 911
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2287635
display id: annotation2287635
version: 1
top level: BBa_K283002
location: https://synbiohub.org/public/igem/BBa_K283002/annotation2287635/range2287635/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K283002/annotation2287635/range2287635/1
type: Range
persistent identity: range2287635
display id: range2287635
version: 1
direction: direction/forward
top level: BBa_K283002
start: 1946
end: 1986
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2287611
display id: annotation2287611
version: 1
top level: BBa_K283002
location: https://synbiohub.org/public/igem/BBa_K283002/annotation2287611/range2287611/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K283002/annotation2287611/range2287611/1
type: Range
persistent identity: range2287611
display id: range2287611
version: 1
direction: direction/reverse
top level: BBa_K283002
start: 209
end: 220
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2287618
display id: annotation2287618
version: 1
top level: BBa_K283002
location: https://synbiohub.org/public/igem/BBa_K283002/annotation2287618/range2287618/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K283002/annotation2287618/range2287618/1
type: Range
persistent identity: range2287618
display id: range2287618
version: 1
direction: direction/forward
top level: BBa_K283002
start: 1008
end: 1048
orientation: inline

BBa_K777104
type: ComponentDefinition
persistent identity: BBa K777104
display id: BBa_K777104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: flhDC operon under the control of constitutive promoter J23106
creator: Team Goettingen
created: 2012-09-18T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The ''flhDC'' operon is the master regulator of motility and chemotaxis in ''E. coli''. FlhDC forms heterotetramers and activates class II operons in concert with sigma factor 70. Among the gene products of class II operons are several components of the flagellum and the alternative sigma factor FliA which is essential for the transcription of class III genes. Here we used a set of 8 constitutive Anderson-promoters from the 2006 Berkeley group to test how different levels of constitutive ''flhDC'' expression affect the motility of ''E. coli''.
mutable notes: * The PstI restriction site in the ''flhDC'' sequence was mutated to CTGCGG using the reverse primer * ''flhDC'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: ** Fwd: 5'-actgaattcgcggccgcttctagatgcatacctccgagttgctg-3' ** Rev: 5'-tcctgcagcggccgctactagttactgcccgggatggcggttgacataagcCgcaggcaaagctgccaacag-3' (the capitalized "C" induces the mutation for removal of the PstI site)
mutable provenance: * The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). * J23106 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23106
component: BBa_K777100
sequence annotation: BBa_K777100
sequence annotation: BBa_J23106
sequence: https://synbiohub.org/public/igem/BBa_K777104_sequence/1

BBa_J23106
type: Component
persistent identity: component2187039
display id: component2187039
version: 1
top level: BBa_K777104
access: public
definition: https://synbiohub.org/public/igem/BBa_J23106/1

BBa_K777100
type: Component
persistent identity: component2187047
display id: component2187047
version: 1
top level: BBa_K777104
access: public
definition: BBa_K777100

BBa_K777100
type: SequenceAnnotation
persistent identity: annotation2187047
display id: annotation2187047
version: 1
top level: BBa_K777104
location: https://synbiohub.org/public/igem/BBa_K777104/annotation2187047/range2187047/1
component: BBa_K777100

https://synbiohub.org/public/igem/BBa_K777104/annotation2187047/range2187047/1
type: Range
persistent identity: range2187047
display id: range2187047
version: 1
direction: direction/reverse
top level: BBa_K777104
start: 42
end: 910
orientation: inline

BBa_J23106
type: SequenceAnnotation
persistent identity: annotation2187039
display id: annotation2187039
version: 1
top level: BBa_K777104
location: https://synbiohub.org/public/igem/BBa_K777104/annotation2187039/range2187039/1
component: BBa_J23106

https://synbiohub.org/public/igem/BBa_K777104/annotation2187039/range2187039/1
type: Range
persistent identity: range2187039
display id: range2187039
version: 1
direction: direction/reverse
top level: BBa_K777104
start: 1
end: 35
orientation: inline

BBa_K108011
type: ComponentDefinition
persistent identity: BBa K108011
display id: BBa_K108011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FliN
creator: Qi Liu
created: 2008-10-22T11:00:00Z
modified: 2015-05-08T01:09:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _222_
m user id: 0
owner id: 2671
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: flagellar motor switching and energizing component
mutable notes: PCR using primers with biobrick prefix and surfix, and then cloned into pSB1AC3.
mutable provenance: E.coli K12 DH10B genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: FliN
sequence: https://synbiohub.org/public/igem/BBa_K108011_sequence/1

FliN
type: SequenceAnnotation
persistent identity: annotation1986956
display id: annotation1986956
version: 1
top level: BBa_K108011
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K108011/annotation1986956/range1986956/1

https://synbiohub.org/public/igem/BBa_K108011/annotation1986956/range1986956/1
type: Range
persistent identity: range1986956
display id: range1986956
version: 1
direction: direction/forward
top level: BBa_K108011
start: 1
end: 411
orientation: inline

BBa_K777109
type: ComponentDefinition
persistent identity: BBa K777109
display id: BBa_K777109
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fliC
creator: Flash Coli
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: * The ''fliC'' gene codes for flagellin. This protein is the subunit that forms the flagellar filament of ''E. Coli''.
mutable notes: * The ''fliC'' gene contained three PstI sites and one SpeI site that had to be removed. These mutations were induced with different primers via overlap-PCR. * ''flhDC'' was amplified from genomic DNA of E. coli DH10B via PCR using the following primers: ** Fwd: ACTGAATTCGCGGCCGCTTCTAGATGCATACCTCCGAGTTGCTG ** Rev: TCCTGCAGCGGCCGCTACTAGTTACTGCCCGGGATGGCGGTTGACATAAGCcGCAGGCAAAGCTGCCAACAG
mutable provenance: * The part was amplified from genomic DNA of E. coli str. K-12 substr. DH10B, complete genome (CP000948.1).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: FliC coding region
sequence annotation: PstI site removed
sequence annotation: TAA stop codon
sequence annotation: ATG start codon
sequence annotation: SpeI site removed
sequence: https://synbiohub.org/public/igem/BBa_K777109_sequence/1

FliC coding region
type: SequenceAnnotation
persistent identity: annotation2184548
display id: annotation2184548
version: 1
top level: BBa_K777109
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K777109/annotation2184548/range2184548/1

https://synbiohub.org/public/igem/BBa_K777109/annotation2184548/range2184548/1
type: Range
persistent identity: range2184548
display id: range2184548
version: 1
direction: direction/forward
top level: BBa_K777109
start: 1
end: 1497
orientation: inline

PstI site removed
type: SequenceAnnotation
persistent identity: annotation2184559
display id: annotation2184559
version: 1
top level: BBa_K777109
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K777109/annotation2184559/range2184559/1

https://synbiohub.org/public/igem/BBa_K777109/annotation2184559/range2184559/1
type: Range
persistent identity: range2184559
display id: range2184559
version: 1
direction: direction/forward
top level: BBa_K777109
start: 921
end: 921
orientation: inline

TAA stop codon
type: SequenceAnnotation
persistent identity: annotation2184550
display id: annotation2184550
version: 1
top level: BBa_K777109
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K777109/annotation2184550/range2184550/1

https://synbiohub.org/public/igem/BBa_K777109/annotation2184550/range2184550/1
type: Range
persistent identity: range2184550
display id: range2184550
version: 1
direction: direction/forward
top level: BBa_K777109
start: 1495
end: 1497
orientation: inline

ATG start codon
type: SequenceAnnotation
persistent identity: annotation2184549
display id: annotation2184549
version: 1
top level: BBa_K777109
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K777109/annotation2184549/range2184549/1

https://synbiohub.org/public/igem/BBa_K777109/annotation2184549/range2184549/1
type: Range
persistent identity: range2184549
display id: range2184549
version: 1
direction: direction/forward
top level: BBa_K777109
start: 1
end: 3
orientation: inline

PstI site removed
type: SequenceAnnotation
persistent identity: annotation2184560
display id: annotation2184560
version: 1
top level: BBa_K777109
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K777109/annotation2184560/range2184560/1

https://synbiohub.org/public/igem/BBa_K777109/annotation2184560/range2184560/1
type: Range
persistent identity: range2184560
display id: range2184560
version: 1
direction: direction/forward
top level: BBa_K777109
start: 1488
end: 1488
orientation: inline

SpeI site removed
type: SequenceAnnotation
persistent identity: annotation2184554
display id: annotation2184554
version: 1
top level: BBa_K777109
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K777109/annotation2184554/range2184554/1

https://synbiohub.org/public/igem/BBa_K777109/annotation2184554/range2184554/1
type: Range
persistent identity: range2184554
display id: range2184554
version: 1
direction: direction/forward
top level: BBa_K777109
start: 552
end: 552
orientation: inline

PstI site removed
type: SequenceAnnotation
persistent identity: annotation2184558
display id: annotation2184558
version: 1
top level: BBa_K777109
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K777109/annotation2184558/range2184558/1

https://synbiohub.org/public/igem/BBa_K777109/annotation2184558/range2184558/1
type: Range
persistent identity: range2184558
display id: range2184558
version: 1
direction: direction/forward
top level: BBa_K777109
start: 876
end: 876
orientation: inline

BBa_K1342001
type: ComponentDefinition
persistent identity: BBa K1342001
display id: BBa_K1342001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: zinTp_AspA
creator: Naoki Saito,Daiki Haraguchi,Yoshiharu Otaki,Ryuhei Minei
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:09:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1717_
m user id: 0
owner id: 22434
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is
mutable notes: soon
mutable provenance: it come from
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: cds
sequence annotation: promoter
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K1342001_sequence/1

cds
type: SequenceAnnotation
persistent identity: annotation2394012
display id: annotation2394012
version: 1
top level: BBa_K1342001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1342001/annotation2394012/range2394012/1

https://synbiohub.org/public/igem/BBa_K1342001/annotation2394012/range2394012/1
type: Range
persistent identity: range2394012
display id: range2394012
version: 1
direction: direction/forward
top level: BBa_K1342001
start: 108
end: 1589
orientation: inline

promoter
type: SequenceAnnotation
persistent identity: annotation2394010
display id: annotation2394010
version: 1
top level: BBa_K1342001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1342001/annotation2394010/range2394010/1

https://synbiohub.org/public/igem/BBa_K1342001/annotation2394010/range2394010/1
type: Range
persistent identity: range2394010
display id: range2394010
version: 1
direction: direction/forward
top level: BBa_K1342001
start: 1
end: 81
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2394011
display id: annotation2394011
version: 1
top level: BBa_K1342001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1342001/annotation2394011/range2394011/1

https://synbiohub.org/public/igem/BBa_K1342001/annotation2394011/range2394011/1
type: Range
persistent identity: range2394011
display id: range2394011
version: 1
direction: direction/forward
top level: BBa_K1342001
start: 82
end: 101
orientation: inline

BBa_K283022
type: ComponentDefinition
persistent identity: BBa K283022
display id: BBa_K283022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLacI-rbs-cheZ
creator: Nelson Chu, W Ling , Shi Lei
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _383_
m user id: 0
owner id: 5494
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part consist of a chez downstream of a pLac promoter. The cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement. The pLac promoter can be repressed by LacI protein
mutable notes: BBa_K283022
mutable provenance: The chez coding region is self-designed The rbs and the pLac promomter are from the registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0010
component: BBa_K094100
component: BBa_B0034
sequence annotation: BBa_R0010
sequence annotation: BBa_B0034
sequence annotation: BBa_K094100
sequence: https://synbiohub.org/public/igem/BBa_K283022_sequence/1

BBa_R0010
type: Component
persistent identity: component2035699
display id: component2035699
version: 1
top level: BBa_K283022
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_K094100
type: Component
persistent identity: component2035709
display id: component2035709
version: 1
top level: BBa_K283022
access: public
definition: BBa_K094100

BBa_B0034
type: Component
persistent identity: component2035707
display id: component2035707
version: 1
top level: BBa_K283022
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2035699
display id: annotation2035699
version: 1
top level: BBa_K283022
location: https://synbiohub.org/public/igem/BBa_K283022/annotation2035699/range2035699/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K283022/annotation2035699/range2035699/1
type: Range
persistent identity: range2035699
display id: range2035699
version: 1
direction: direction/forward
top level: BBa_K283022
start: 1
end: 200
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2035707
display id: annotation2035707
version: 1
top level: BBa_K283022
location: https://synbiohub.org/public/igem/BBa_K283022/annotation2035707/range2035707/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K283022/annotation2035707/range2035707/1
type: Range
persistent identity: range2035707
display id: range2035707
version: 1
direction: direction/reverse
top level: BBa_K283022
start: 209
end: 220
orientation: inline

BBa_K094100
type: SequenceAnnotation
persistent identity: annotation2035709
display id: annotation2035709
version: 1
top level: BBa_K283022
location: https://synbiohub.org/public/igem/BBa_K283022/annotation2035709/range2035709/1
component: BBa_K094100

https://synbiohub.org/public/igem/BBa_K283022/annotation2035709/range2035709/1
type: Range
persistent identity: range2035709
display id: range2035709
version: 1
direction: direction/reverse
top level: BBa_K283022
start: 227
end: 921
orientation: inline

cheZ Unit
type: ComponentDefinition
persistent identity: BBa K518007
display id: BBa_K518007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cheZ expression cassette (no promoter)
creator: Masato Ohgishi
created: 2011-09-18T11:00:00Z
modified: 2015-05-08T01:12:33Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _683_
m user id: 0
owner id: 8305
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: CheZ is responsible for the dephosphorylation of flagellum-regulating protein CheY. It has been reported that cheZ-/- strain has a higher frequency of direction change and thus a narrower range of mobility. With a promoter of your interest, this device rescues the mobility of cheZ-/- cells.
mutable notes: None.
mutable provenance: cheZ CDS is cloned from Escherichia coli JM109 strain. Other sequences are from BioBrick parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K518005
component: BBa_B0014
component: BBa_B0032
sequence annotation: BBa_B0014
sequence annotation: BBa_B0032
sequence annotation: BBa_K518005
sequence: https://synbiohub.org/public/igem/BBa_K518007_sequence/1

BBa_K518005
type: Component
persistent identity: component2134261
display id: component2134261
version: 1
top level: cheZ Unit
access: public
definition: BBa_K518005

BBa_B0014
type: Component
persistent identity: component2134268
display id: component2134268
version: 1
top level: cheZ Unit
access: public
definition: https://synbiohub.org/public/igem/BBa_B0014/1

BBa_B0032
type: Component
persistent identity: component2134259
display id: component2134259
version: 1
top level: cheZ Unit
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2134268
display id: annotation2134268
version: 1
top level: cheZ Unit
location: https://synbiohub.org/public/igem/BBa_K518007/annotation2134268/range2134268/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K518007/annotation2134268/range2134268/1
type: Range
persistent identity: range2134268
display id: range2134268
version: 1
direction: direction/reverse
top level: cheZ Unit
start: 835
end: 929
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2134259
display id: annotation2134259
version: 1
top level: cheZ Unit
location: https://synbiohub.org/public/igem/BBa_K518007/annotation2134259/range2134259/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K518007/annotation2134259/range2134259/1
type: Range
persistent identity: range2134259
display id: range2134259
version: 1
direction: direction/forward
top level: cheZ Unit
start: 1
end: 13
orientation: inline

BBa_K518005
type: SequenceAnnotation
persistent identity: annotation2134261
display id: annotation2134261
version: 1
top level: cheZ Unit
location: https://synbiohub.org/public/igem/BBa_K518007/annotation2134261/range2134261/1
component: BBa_K518005

https://synbiohub.org/public/igem/BBa_K518007/annotation2134261/range2134261/1
type: Range
persistent identity: range2134261
display id: range2134261
version: 1
direction: direction/reverse
top level: cheZ Unit
start: 22
end: 826
orientation: inline

BBa_K777114
type: ComponentDefinition
persistent identity: BBa K777114
display id: BBa_K777114
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: motA under the control of constitutive promoter J23100
creator: Team Goettingen
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The ''motA'' gene codes for a part of the flagellar motor complex. MotA proteins represent the proton conducting component of this molecular machine. Thus it is necessary for flagellar rotation. Here we used 3 different constitutive [http://partsregistry.org/Part:BBa_J23100 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''motA'' expression affect the motility or flagellum size of ''E. coli''.
mutable notes: *''motA'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: **Fwd: 5'-gtttcttcgaattcgcggccgcttctagatgaccggtggaagccttgg-3' **Rev: 5'-gtttcttcctgcagcggccgctactagtatcatgcttcctcggttgtcg-3'
mutable provenance: * ''motA'' was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). * J23100 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23100
component: BBa_K777113
sequence annotation: BBa_K777113
sequence annotation: BBa_J23100
sequence: https://synbiohub.org/public/igem/BBa_K777114_sequence/1

BBa_J23100
type: Component
persistent identity: component2189705
display id: component2189705
version: 1
top level: BBa_K777114
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_K777113
type: Component
persistent identity: component2189707
display id: component2189707
version: 1
top level: BBa_K777114
access: public
definition: BBa_K777113

BBa_K777113
type: SequenceAnnotation
persistent identity: annotation2189707
display id: annotation2189707
version: 1
top level: BBa_K777114
location: https://synbiohub.org/public/igem/BBa_K777114/annotation2189707/range2189707/1
component: BBa_K777113

https://synbiohub.org/public/igem/BBa_K777114/annotation2189707/range2189707/1
type: Range
persistent identity: range2189707
display id: range2189707
version: 1
direction: direction/reverse
top level: BBa_K777114
start: 42
end: 872
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2189705
display id: annotation2189705
version: 1
top level: BBa_K777114
location: https://synbiohub.org/public/igem/BBa_K777114/annotation2189705/range2189705/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K777114/annotation2189705/range2189705/1
type: Range
persistent identity: range2189705
display id: range2189705
version: 1
direction: direction/reverse
top level: BBa_K777114
start: 1
end: 35
orientation: inline

BBa_K283023
type: ComponentDefinition
persistent identity: BBa K283023
display id: BBa_K283023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: plux-rbs-CI-terminator-plambda(R-O12)-rbs-cheZ-terminator
creator: Liu Chenli
created: 2009-10-10T11:00:00Z
modified: 2015-05-08T01:11:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _383_
m user id: 0
owner id: 5494
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part contains a strong rbs and a TetR tagged with a LVA degradation tail. The TetR repressor can be used to repress ptet promoter and the TetR coding region do not contain any terminator
mutable notes: BBa_K283029
mutable provenance: BBa_K283029
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K094013
component: BBa_B0034
component: BBa_R0062
component: BBa_K094002
component: BBa_K094011
component: BBa_K094100
sequence annotation: BBa_K094100
sequence annotation: BBa_K094011
sequence annotation: BBa_K094013
sequence annotation: BBa_R0062
sequence annotation: BBa_B0034
sequence annotation: BBa_K094002
sequence: https://synbiohub.org/public/igem/BBa_K283023_sequence/1

BBa_K094013
type: Component
persistent identity: component2054862
display id: component2054862
version: 1
top level: BBa_K283023
access: public
definition: https://synbiohub.org/public/igem/BBa_K094013/1

BBa_B0034
type: Component
persistent identity: component2054866
display id: component2054866
version: 1
top level: BBa_K283023
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0062
type: Component
persistent identity: component2054855
display id: component2054855
version: 1
top level: BBa_K283023
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_K094002
type: Component
persistent identity: component2054864
display id: component2054864
version: 1
top level: BBa_K283023
access: public
definition: https://synbiohub.org/public/igem/BBa_K094002/1

BBa_B0034
type: Component
persistent identity: component2054860
display id: component2054860
version: 1
top level: BBa_K283023
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K094011
type: Component
persistent identity: component2054861
display id: component2054861
version: 1
top level: BBa_K283023
access: public
definition: https://synbiohub.org/public/igem/BBa_K094011/1

BBa_K094013
type: Component
persistent identity: component2054869
display id: component2054869
version: 1
top level: BBa_K283023
access: public
definition: https://synbiohub.org/public/igem/BBa_K094013/1

BBa_K094100
type: Component
persistent identity: component2054868
display id: component2054868
version: 1
top level: BBa_K283023
access: public
definition: BBa_K094100

BBa_K094100
type: SequenceAnnotation
persistent identity: annotation2054868
display id: annotation2054868
version: 1
top level: BBa_K283023
location: https://synbiohub.org/public/igem/BBa_K283023/annotation2054868/range2054868/1
component: BBa_K094100

https://synbiohub.org/public/igem/BBa_K283023/annotation2054868/range2054868/1
type: Range
persistent identity: range2054868
display id: range2054868
version: 1
direction: direction/reverse
top level: BBa_K283023
start: 1827
end: 2521
orientation: inline

BBa_K094011
type: SequenceAnnotation
persistent identity: annotation2054861
display id: annotation2054861
version: 1
top level: BBa_K283023
location: https://synbiohub.org/public/igem/BBa_K283023/annotation2054861/range2054861/1
component: BBa_K094011

https://synbiohub.org/public/igem/BBa_K283023/annotation2054861/range2054861/1
type: Range
persistent identity: range2054861
display id: range2054861
version: 1
direction: direction/reverse
top level: BBa_K283023
start: 82
end: 1590
orientation: inline

BBa_K094013
type: SequenceAnnotation
persistent identity: annotation2054869
display id: annotation2054869
version: 1
top level: BBa_K283023
location: https://synbiohub.org/public/igem/BBa_K283023/annotation2054869/range2054869/1
component: BBa_K094013

https://synbiohub.org/public/igem/BBa_K283023/annotation2054869/range2054869/1
type: Range
persistent identity: range2054869
display id: range2054869
version: 1
direction: direction/reverse
top level: BBa_K283023
start: 2530
end: 2623
orientation: inline

BBa_K094013
type: SequenceAnnotation
persistent identity: annotation2054862
display id: annotation2054862
version: 1
top level: BBa_K283023
location: https://synbiohub.org/public/igem/BBa_K283023/annotation2054862/range2054862/1
component: BBa_K094013

https://synbiohub.org/public/igem/BBa_K283023/annotation2054862/range2054862/1
type: Range
persistent identity: range2054862
display id: range2054862
version: 1
direction: direction/reverse
top level: BBa_K283023
start: 1599
end: 1692
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2054855
display id: annotation2054855
version: 1
top level: BBa_K283023
location: https://synbiohub.org/public/igem/BBa_K283023/annotation2054855/range2054855/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K283023/annotation2054855/range2054855/1
type: Range
persistent identity: range2054855
display id: range2054855
version: 1
direction: direction/forward
top level: BBa_K283023
start: 1
end: 55
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2054866
display id: annotation2054866
version: 1
top level: BBa_K283023
location: https://synbiohub.org/public/igem/BBa_K283023/annotation2054866/range2054866/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K283023/annotation2054866/range2054866/1
type: Range
persistent identity: range2054866
display id: range2054866
version: 1
direction: direction/reverse
top level: BBa_K283023
start: 1809
end: 1820
orientation: inline

BBa_K094002
type: SequenceAnnotation
persistent identity: annotation2054864
display id: annotation2054864
version: 1
top level: BBa_K283023
location: https://synbiohub.org/public/igem/BBa_K283023/annotation2054864/range2054864/1
component: BBa_K094002

https://synbiohub.org/public/igem/BBa_K283023/annotation2054864/range2054864/1
type: Range
persistent identity: range2054864
display id: range2054864
version: 1
direction: direction/reverse
top level: BBa_K283023
start: 1701
end: 1800
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2054860
display id: annotation2054860
version: 1
top level: BBa_K283023
location: https://synbiohub.org/public/igem/BBa_K283023/annotation2054860/range2054860/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K283023/annotation2054860/range2054860/1
type: Range
persistent identity: range2054860
display id: range2054860
version: 1
direction: direction/reverse
top level: BBa_K283023
start: 64
end: 75
orientation: inline

BBa_K777122
type: ComponentDefinition
persistent identity: BBa K777122
display id: BBa_K777122
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: yhjH under the control of constitutive promoter J23100
creator: Team Goettingen
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The product of this gene is a phosphodiesterase which is involved in regulating the levels of c-di-GMP, a bacterial signaling molecule. High levels of c-di-GMP promote biofilm synthesis and inhibit flagella by binding to YcgR which then acts as a "brake" on FliG and FliM. Levels of c-di-GMP are elevated and motility is reduced in ''yhjH''-mutants. Here we used 3 different constitutive [http://partsregistry.org/Part:BBa_J23100 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''yhjH'' expression affect the motility of ''E. coli''.
mutable notes: * ''yhjH'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: ** Fwd: 5'-gtttcttcgaattcgcggccgcttctagatgataaggcaggttatccagc-3' ** Rev: 5'-gtttcttcctgcagcggccgctactagtattatagcgccagaaccgccgtattcagc-3'
mutable provenance: * ''yhjH'' was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). * J23100 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_K777121
component: BBa_J23100
sequence annotation: BBa_J23100
sequence annotation: BBa_K777121
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K777122_sequence/1

BBa_B0034
type: Component
persistent identity: component2191155
display id: component2191155
version: 1
top level: BBa_K777122
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K777121
type: Component
persistent identity: component2191157
display id: component2191157
version: 1
top level: BBa_K777122
access: public
definition: BBa_K777121

BBa_J23100
type: Component
persistent identity: component2191153
display id: component2191153
version: 1
top level: BBa_K777122
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2191153
display id: annotation2191153
version: 1
top level: BBa_K777122
location: https://synbiohub.org/public/igem/BBa_K777122/annotation2191153/range2191153/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K777122/annotation2191153/range2191153/1
type: Range
persistent identity: range2191153
display id: range2191153
version: 1
direction: direction/reverse
top level: BBa_K777122
start: 1
end: 35
orientation: inline

BBa_K777121
type: SequenceAnnotation
persistent identity: annotation2191157
display id: annotation2191157
version: 1
top level: BBa_K777122
location: https://synbiohub.org/public/igem/BBa_K777122/annotation2191157/range2191157/1
component: BBa_K777121

https://synbiohub.org/public/igem/BBa_K777122/annotation2191157/range2191157/1
type: Range
persistent identity: range2191157
display id: range2191157
version: 1
direction: direction/reverse
top level: BBa_K777122
start: 62
end: 829
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2191155
display id: annotation2191155
version: 1
top level: BBa_K777122
location: https://synbiohub.org/public/igem/BBa_K777122/annotation2191155/range2191155/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K777122/annotation2191155/range2191155/1
type: Range
persistent identity: range2191155
display id: range2191155
version: 1
direction: direction/reverse
top level: BBa_K777122
start: 44
end: 55
orientation: inline

BBa_K819016
type: ComponentDefinition
persistent identity: BBa K819016
display id: BBa_K819016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Luminesensor repressible SulA408 promoter+CheZ
creator: YU Zhou
created: 2012-09-15T11:00:00Z
modified: 2015-05-08T01:13:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1077_
m user id: 0
owner id: 13785
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: CheZ coding sequence ligated to the Luminesensor repressible SulA408 promoter.
mutable notes: CheZ coding sequence ligated to the Luminesensor repressible SulA408 promoter.
mutable provenance: CheZ is got from E.Coli strain MG1655 by colony PCR
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
sequence annotation: CheZ
sequence annotation: SulA408 Promoter
sequence annotation: B0034
sequence: https://synbiohub.org/public/igem/BBa_K819016_sequence/1

CheZ
type: SequenceAnnotation
persistent identity: annotation2197285
display id: annotation2197285
version: 1
top level: BBa_K819016
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K819016/annotation2197285/range2197285/1

https://synbiohub.org/public/igem/BBa_K819016/annotation2197285/range2197285/1
type: Range
persistent identity: range2197285
display id: range2197285
version: 1
direction: direction/forward
top level: BBa_K819016
start: 108
end: 752
orientation: inline

SulA408 Promoter
type: SequenceAnnotation
persistent identity: annotation2197277
display id: annotation2197277
version: 1
top level: BBa_K819016
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K819016/annotation2197277/range2197277/1

https://synbiohub.org/public/igem/BBa_K819016/annotation2197277/range2197277/1
type: Range
persistent identity: range2197277
display id: range2197277
version: 1
direction: direction/forward
top level: BBa_K819016
start: 1
end: 81
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2197281
display id: annotation2197281
version: 1
top level: BBa_K819016
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K819016/annotation2197281/range2197281/1

https://synbiohub.org/public/igem/BBa_K819016/annotation2197281/range2197281/1
type: Range
persistent identity: range2197281
display id: range2197281
version: 1
direction: direction/forward
top level: BBa_K819016
start: 90
end: 101
orientation: inline

BBa_K777106
type: ComponentDefinition
persistent identity: BBa K777106
display id: BBa_K777106
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: flhDC operon under the control of constitutive promoter J23112
creator: Team Goettingen
created: 2012-09-18T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The ''flhDC'' operon is the master regulator of motility and chemotaxis in ''E. coli''. FlhDC forms heterotetramers and activates class II operons in concert with sigma factor 70. Among the gene products of class II operons are several components of the flagellum and the alternative sigma factor FliA which is essential for the transcription of class III genes. Here we used a set of 8 constitutive Anderson-promoters from the 2006 Berkeley group to test how different levels of constitutive ''flhDC'' expression affect the motility of ''E. coli''.
mutable notes: *The PstI restriction site in the ''flhDC'' sequence was mutated to CTGCGG using the reverse primer. *''flhDC'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: **Fwd: 5'-actgaattcgcggccgcttctagatgcatacctccgagttgctg-3' **Rev: 5'-tcctgcagcggccgctactagttactgcccgggatggcggttgacataagcCgcaggcaaagctgccaacag-3' (the capitalized "C" induces the mutation for removal of the PstI site)
mutable provenance: *The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). *J23112 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K777100
component: BBa_J23112
sequence annotation: BBa_K777100
sequence annotation: BBa_J23112
sequence: https://synbiohub.org/public/igem/BBa_K777106_sequence/1

BBa_K777100
type: Component
persistent identity: component2187094
display id: component2187094
version: 1
top level: BBa_K777106
access: public
definition: BBa_K777100

BBa_J23112
type: Component
persistent identity: component2187086
display id: component2187086
version: 1
top level: BBa_K777106
access: public
definition: https://synbiohub.org/public/igem/BBa_J23112/1

BBa_K777100
type: SequenceAnnotation
persistent identity: annotation2187094
display id: annotation2187094
version: 1
top level: BBa_K777106
location: https://synbiohub.org/public/igem/BBa_K777106/annotation2187094/range2187094/1
component: BBa_K777100

https://synbiohub.org/public/igem/BBa_K777106/annotation2187094/range2187094/1
type: Range
persistent identity: range2187094
display id: range2187094
version: 1
direction: direction/reverse
top level: BBa_K777106
start: 42
end: 910
orientation: inline

BBa_J23112
type: SequenceAnnotation
persistent identity: annotation2187086
display id: annotation2187086
version: 1
top level: BBa_K777106
location: https://synbiohub.org/public/igem/BBa_K777106/annotation2187086/range2187086/1
component: BBa_J23112

https://synbiohub.org/public/igem/BBa_K777106/annotation2187086/range2187086/1
type: Range
persistent identity: range2187086
display id: range2187086
version: 1
direction: direction/reverse
top level: BBa_K777106
start: 1
end: 35
orientation: inline

BBa_K777111
type: ComponentDefinition
persistent identity: BBa K777111
display id: BBa_K777111
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fliC under the control of constitutive promoter J23112
creator: Team Goettingen
created: 2012-09-21T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The ''fliC'' gene codes for flagellin. This protein is the subunit that forms the flagellar filament of ''E. coli''. Here we used 3 different constitutive [http://partsregistry.org/Part:BBa_J23100 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''fliC'' expression affect the motility or flagellum size of ''E. coli''.
mutable notes: * The ''fliC'' gene contained three PstI sites and one SpeI site that had to be removed. These mutations were induced with different primers via overlap-PCR.
mutable provenance: * The ''fliC'' gene was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K777109
component: BBa_J23112
sequence annotation: BBa_J23112
sequence annotation: BBa_K777109
sequence: https://synbiohub.org/public/igem/BBa_K777111_sequence/1

BBa_K777109
type: Component
persistent identity: component2192728
display id: component2192728
version: 1
top level: BBa_K777111
access: public
definition: BBa_K777109

BBa_J23112
type: Component
persistent identity: component2192720
display id: component2192720
version: 1
top level: BBa_K777111
access: public
definition: https://synbiohub.org/public/igem/BBa_J23112/1

BBa_J23112
type: SequenceAnnotation
persistent identity: annotation2192720
display id: annotation2192720
version: 1
top level: BBa_K777111
location: https://synbiohub.org/public/igem/BBa_K777111/annotation2192720/range2192720/1
component: BBa_J23112

https://synbiohub.org/public/igem/BBa_K777111/annotation2192720/range2192720/1
type: Range
persistent identity: range2192720
display id: range2192720
version: 1
direction: direction/reverse
top level: BBa_K777111
start: 1
end: 35
orientation: inline

BBa_K777109
type: SequenceAnnotation
persistent identity: annotation2192728
display id: annotation2192728
version: 1
top level: BBa_K777111
location: https://synbiohub.org/public/igem/BBa_K777111/annotation2192728/range2192728/1
component: BBa_K777109

https://synbiohub.org/public/igem/BBa_K777111/annotation2192728/range2192728/1
type: Range
persistent identity: range2192728
display id: range2192728
version: 1
direction: direction/reverse
top level: BBa_K777111
start: 42
end: 1538
orientation: inline

BBa_K108010
type: ComponentDefinition
persistent identity: BBa K108010
display id: BBa_K108010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FliM
creator: Qi Liu
created: 2008-10-22T11:00:00Z
modified: 2015-05-08T01:09:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _222_
m user id: 0
owner id: 2671
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: flagellar motor switching and energizing component
mutable notes: PCR using primers with biobrick prefix and surfix, and then cloned into pSB1AC3.
mutable provenance: E.coli K12 DH10B genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: FliM
sequence: https://synbiohub.org/public/igem/BBa_K108010_sequence/1

FliM
type: SequenceAnnotation
persistent identity: annotation1986955
display id: annotation1986955
version: 1
top level: BBa_K108010
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K108010/annotation1986955/range1986955/1

https://synbiohub.org/public/igem/BBa_K108010/annotation1986955/range1986955/1
type: Range
persistent identity: range1986955
display id: range1986955
version: 1
direction: direction/forward
top level: BBa_K108010
start: 1
end: 1002
orientation: inline

BBa_K1342002
type: ComponentDefinition
persistent identity: BBa K1342002
display id: BBa_K1342002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AspA LVA(-)
creator: Naoki Saito,Daiki Haraguchi,Yoshiharu Otaki,Ryuhei Minei
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:09:59Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1717_
m user id: 0
owner id: 22434
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is
mutable notes: soon
mutable provenance: it
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cds
sequence: https://synbiohub.org/public/igem/BBa_K1342002_sequence/1

cds
type: SequenceAnnotation
persistent identity: annotation2394009
display id: annotation2394009
version: 1
top level: BBa_K1342002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1342002/annotation2394009/range2394009/1

https://synbiohub.org/public/igem/BBa_K1342002/annotation2394009/range2394009/1
type: Range
persistent identity: range2394009
display id: range2394009
version: 1
direction: direction/forward
top level: BBa_K1342002
start: 1
end: 1482
orientation: inline

BBa_K777121
type: ComponentDefinition
persistent identity: BBa K777121
display id: BBa_K777121
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: yhjH
creator: Flash Coli
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:17Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: The product of this gene is a phosphodiesterase which is involved in regulating the levels of c-di-GMP, a bacterial signaling molecule. High levels of c-di-GMP promote biofilm synthesis and inhibit flagella by binding to YcgR which then acts as a "brake" on FliG and FliM. Levels of c-di-GMP are elevated and motility is reduced in ''yhjH''-mutants.
mutable notes: * ''yhjH'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: ** Fwd: 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGATAAGGCAGGTTATCCAGC-3' ** Rev: 5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATAGCGCCAGAACCGCCGTATTCAGC-3'
mutable provenance: The part was amplified from genomic DNA of E. coli str. K-12 substr. DH10B, complete genome (CP000948.1).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: YhjH coding sequence
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K777121_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2184530
display id: annotation2184530
version: 1
top level: BBa_K777121
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K777121/annotation2184530/range2184530/1

https://synbiohub.org/public/igem/BBa_K777121/annotation2184530/range2184530/1
type: Range
persistent identity: range2184530
display id: range2184530
version: 1
direction: direction/forward
top level: BBa_K777121
start: 768
end: 768
orientation: inline

YhjH coding sequence
type: SequenceAnnotation
persistent identity: annotation2184528
display id: annotation2184528
version: 1
top level: BBa_K777121
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K777121/annotation2184528/range2184528/1

https://synbiohub.org/public/igem/BBa_K777121/annotation2184528/range2184528/1
type: Range
persistent identity: range2184528
display id: range2184528
version: 1
direction: direction/forward
top level: BBa_K777121
start: 1
end: 768
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2184529
display id: annotation2184529
version: 1
top level: BBa_K777121
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K777121/annotation2184529/range2184529/1

https://synbiohub.org/public/igem/BBa_K777121/annotation2184529/range2184529/1
type: Range
persistent identity: range2184529
display id: range2184529
version: 1
direction: direction/forward
top level: BBa_K777121
start: 1
end: 1
orientation: inline

BBa_K108005
type: ComponentDefinition
persistent identity: BBa K108005
display id: BBa_K108005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FlgH
creator: Qi Liu
created: 2008-10-22T11:00:00Z
modified: 2015-05-08T01:09:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _222_
m user id: 0
owner id: 2671
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: flagellar protein of basal-body outer-membrane L ring
mutable notes: PCR using primers with biobrick prefix and surfix, and then cloned into pSB1AC3.
mutable provenance: E.coli K12 DH10B genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: FlgH
sequence: https://synbiohub.org/public/igem/BBa_K108005_sequence/1

FlgH
type: SequenceAnnotation
persistent identity: annotation1986950
display id: annotation1986950
version: 1
top level: BBa_K108005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K108005/annotation1986950/range1986950/1

https://synbiohub.org/public/igem/BBa_K108005/annotation1986950/range1986950/1
type: Range
persistent identity: range1986950
display id: range1986950
version: 1
direction: direction/forward
top level: BBa_K108005
start: 1
end: 696
orientation: inline

BBa_K777115
type: ComponentDefinition
persistent identity: BBa K777115
display id: BBa_K777115
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: motA under the control of constitutive promoter J23112
creator: Team Goettingen
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The ''motA'' gene codes for a part of the flagellar motor complex. MotA proteins represent the proton conducting component of this molecular machine. Thus it is necessary for flagellar rotation. Here we used 3 different constitutive [http://partsregistry.org/Part:BBa_J23100 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''motA'' expression affect the motility or flagellum size of ''E. coli''.
mutable notes: *''motA'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: **Fwd: 5'-gtttcttcgaattcgcggccgcttctagatgaccggtggaagccttgg-3' **Rev: 5'-gtttcttcctgcagcggccgctactagtatcatgcttcctcggttgtcg-3'
mutable provenance: * ''motA'' was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). * J23100 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23112
component: BBa_K777113
sequence annotation: BBa_J23112
sequence annotation: BBa_K777113
sequence: https://synbiohub.org/public/igem/BBa_K777115_sequence/1

BBa_J23112
type: Component
persistent identity: component2189726
display id: component2189726
version: 1
top level: BBa_K777115
access: public
definition: https://synbiohub.org/public/igem/BBa_J23112/1

BBa_K777113
type: Component
persistent identity: component2189728
display id: component2189728
version: 1
top level: BBa_K777115
access: public
definition: BBa_K777113

BBa_J23112
type: SequenceAnnotation
persistent identity: annotation2189726
display id: annotation2189726
version: 1
top level: BBa_K777115
location: https://synbiohub.org/public/igem/BBa_K777115/annotation2189726/range2189726/1
component: BBa_J23112

https://synbiohub.org/public/igem/BBa_K777115/annotation2189726/range2189726/1
type: Range
persistent identity: range2189726
display id: range2189726
version: 1
direction: direction/reverse
top level: BBa_K777115
start: 1
end: 35
orientation: inline

BBa_K777113
type: SequenceAnnotation
persistent identity: annotation2189728
display id: annotation2189728
version: 1
top level: BBa_K777115
location: https://synbiohub.org/public/igem/BBa_K777115/annotation2189728/range2189728/1
component: BBa_K777113

https://synbiohub.org/public/igem/BBa_K777115/annotation2189728/range2189728/1
type: Range
persistent identity: range2189728
display id: range2189728
version: 1
direction: direction/reverse
top level: BBa_K777115
start: 42
end: 872
orientation: inline

BBa_K777107
type: ComponentDefinition
persistent identity: BBa K777107
display id: BBa_K777107
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: flhDC operon under the control of constitutive promoter J23113
creator: Team Goettingen
created: 2012-09-18T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The ''flhDC'' operon is the master regulator of motility and chemotaxis in ''E. coli''. FlhDC forms heterotetramers and activates class II operons in concert with sigma factor 70. Among the gene products of class II operons are several components of the flagellum and the alternative sigma factor FliA which is essential for the transcription of class III genes. Here we used a set of 8 constitutive Anderson-promoters from the 2006 Berkeley group to test how different levels of constitutive ''flhDC'' expression affect the motility of ''E. coli''.
mutable notes: *The PstI restriction site in the ''flhDC'' sequence was mutated to CTGCGG using the reverse primer. *''flhDC'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: **Fwd: 5'-actgaattcgcggccgcttctagatgcatacctccgagttgctg-3' **Rev: 5'-tcctgcagcggccgctactagttactgcccgggatggcggttgacataagcCgcaggcaaagctgccaacag-3' (the capitalized "C" induces the mutation for removal of the PstI site)
mutable provenance: *The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). *J23113 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K777100
component: BBa_J23113
sequence annotation: BBa_J23113
sequence annotation: BBa_K777100
sequence: https://synbiohub.org/public/igem/BBa_K777107_sequence/1

BBa_K777100
type: Component
persistent identity: component2187103
display id: component2187103
version: 1
top level: BBa_K777107
access: public
definition: BBa_K777100

BBa_J23113
type: Component
persistent identity: component2187095
display id: component2187095
version: 1
top level: BBa_K777107
access: public
definition: https://synbiohub.org/public/igem/BBa_J23113/1

BBa_J23113
type: SequenceAnnotation
persistent identity: annotation2187095
display id: annotation2187095
version: 1
top level: BBa_K777107
location: https://synbiohub.org/public/igem/BBa_K777107/annotation2187095/range2187095/1
component: BBa_J23113

https://synbiohub.org/public/igem/BBa_K777107/annotation2187095/range2187095/1
type: Range
persistent identity: range2187095
display id: range2187095
version: 1
direction: direction/reverse
top level: BBa_K777107
start: 1
end: 35
orientation: inline

BBa_K777100
type: SequenceAnnotation
persistent identity: annotation2187103
display id: annotation2187103
version: 1
top level: BBa_K777107
location: https://synbiohub.org/public/igem/BBa_K777107/annotation2187103/range2187103/1
component: BBa_K777100

https://synbiohub.org/public/igem/BBa_K777107/annotation2187103/range2187103/1
type: Range
persistent identity: range2187103
display id: range2187103
version: 1
direction: direction/reverse
top level: BBa_K777107
start: 42
end: 910
orientation: inline

BBa_I712010
type: ComponentDefinition
persistent identity: BBa I712010
display id: BBa_I712010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CD4 sequence without signal peptide
creator: peter cimermancic
created: 2007-10-22T11:00:00Z
modified: 2015-08-31T04:07:45Z
discontinued: false
dominant: false
group u list: _130_
m user id: 0
owner id: 1850
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: It`s a chemokine receptor protein.
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I712010_sequence/1

BBa_K283016
type: ComponentDefinition
persistent identity: BBa K283016
display id: BBa_K283016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLacI-rbs-cheZ_histag-terminators
creator: W Ling , Nelson Chu, Shi Lei
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _383_
m user id: 0
owner id: 5494
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The pLac promoter can be repressed by LacI protein. The cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement and is tagged by 6-Histidine tail.
mutable notes: BBa_K283016
mutable provenance: The chez-Histag coding region is self-designed The pLac promoter, rbs and the double terminator are from the registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_R0010
component: BBa_B0012
component: BBa_K283008
component: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_R0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_K283008
sequence: https://synbiohub.org/public/igem/BBa_K283016_sequence/1

BBa_B0034
type: Component
persistent identity: component2035644
display id: component2035644
version: 1
top level: BBa_K283016
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0010
type: Component
persistent identity: component2035636
display id: component2035636
version: 1
top level: BBa_K283016
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0012
type: Component
persistent identity: component2035651
display id: component2035651
version: 1
top level: BBa_K283016
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_K283008
type: Component
persistent identity: component2035648
display id: component2035648
version: 1
top level: BBa_K283016
access: public
definition: BBa_K283008

BBa_B0010
type: Component
persistent identity: component2035649
display id: component2035649
version: 1
top level: BBa_K283016
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2035644
display id: annotation2035644
version: 1
top level: BBa_K283016
location: https://synbiohub.org/public/igem/BBa_K283016/annotation2035644/range2035644/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K283016/annotation2035644/range2035644/1
type: Range
persistent identity: range2035644
display id: range2035644
version: 1
direction: direction/reverse
top level: BBa_K283016
start: 209
end: 220
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2035636
display id: annotation2035636
version: 1
top level: BBa_K283016
location: https://synbiohub.org/public/igem/BBa_K283016/annotation2035636/range2035636/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K283016/annotation2035636/range2035636/1
type: Range
persistent identity: range2035636
display id: range2035636
version: 1
direction: direction/forward
top level: BBa_K283016
start: 1
end: 200
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2035649
display id: annotation2035649
version: 1
top level: BBa_K283016
location: https://synbiohub.org/public/igem/BBa_K283016/annotation2035649/range2035649/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K283016/annotation2035649/range2035649/1
type: Range
persistent identity: range2035649
display id: range2035649
version: 1
direction: direction/forward
top level: BBa_K283016
start: 948
end: 1027
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2035651
display id: annotation2035651
version: 1
top level: BBa_K283016
location: https://synbiohub.org/public/igem/BBa_K283016/annotation2035651/range2035651/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K283016/annotation2035651/range2035651/1
type: Range
persistent identity: range2035651
display id: range2035651
version: 1
direction: direction/forward
top level: BBa_K283016
start: 1036
end: 1076
orientation: inline

BBa_K283008
type: SequenceAnnotation
persistent identity: annotation2035648
display id: annotation2035648
version: 1
top level: BBa_K283016
location: https://synbiohub.org/public/igem/BBa_K283016/annotation2035648/range2035648/1
component: BBa_K283008

https://synbiohub.org/public/igem/BBa_K283016/annotation2035648/range2035648/1
type: Range
persistent identity: range2035648
display id: range2035648
version: 1
direction: direction/reverse
top level: BBa_K283016
start: 227
end: 939
orientation: inline

BBa_K108003
type: ComponentDefinition
persistent identity: BBa K108003
display id: BBa_K108003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FlgB
creator: Qi Liu
created: 2008-10-19T11:00:00Z
modified: 2015-05-08T01:09:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _222_
m user id: 0
owner id: 2671
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: flagellar component of cell-proximal portion of basal-body rod
mutable notes: PCR using primers with biobrick prefix and surfix, and then cloned into pSB1AC3.
mutable provenance: E.coli K12 DH10B genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: FlgB
sequence: https://synbiohub.org/public/igem/BBa_K108003_sequence/1

FlgB
type: SequenceAnnotation
persistent identity: annotation1986707
display id: annotation1986707
version: 1
top level: BBa_K108003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K108003/annotation1986707/range1986707/1

https://synbiohub.org/public/igem/BBa_K108003/annotation1986707/range1986707/1
type: Range
persistent identity: range1986707
display id: range1986707
version: 1
direction: direction/forward
top level: BBa_K108003
start: 1
end: 414
orientation: inline

BBa_I712002
type: ComponentDefinition
persistent identity: BBa I712002
display id: BBa_I712002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CCR5
creator: Andrej Ondracka
created: 2007-10-20T11:00:00Z
modified: 2015-08-31T04:07:45Z
discontinued: false
dominant: false
group u list: _130_
m user id: 0
owner id: 1896
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Chemokine (C-C motif) receptor 5, a HIV-1 co-receptor.
mutable notes: The part is cloned into BBa_J52017, a vector with eukaryontic terminator. PstI and EcoRI restriction sites inside CCR5 gene were mutated.
mutable provenance: CCR5 gene was obtained from Origene.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: CCR5
sequence: https://synbiohub.org/public/igem/BBa_I712002_sequence/1

CCR5
type: SequenceAnnotation
persistent identity: annotation1951768
display id: annotation1951768
version: 1
top level: BBa_I712002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I712002/annotation1951768/range1951768/1

https://synbiohub.org/public/igem/BBa_I712002/annotation1951768/range1951768/1
type: Range
persistent identity: range1951768
display id: range1951768
version: 1
direction: direction/forward
top level: BBa_I712002
start: 1
end: 1059
orientation: inline

BBa_K283021
type: ComponentDefinition
persistent identity: BBa K283021
display id: BBa_K283021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTet-rbs-cheZ-termintors
creator: Liu Chenli
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _383_
m user id: 0
owner id: 5494
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The chez is constitutively expressed under the pTet promoter. The pTet promoter can be repressed by TetR repressor protein. The cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement.
mutable notes: BBa_K283021
mutable provenance: The chez coding region is self-designed The pTet promoter, rbs and the terminator are from the registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_R0040
component: BBa_K094100
component: BBa_B0010
component: BBa_B0034
component: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_K094100
sequence annotation: BBa_B0034
sequence annotation: BBa_R0040
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K283021_sequence/1

BBa_R0040
type: Component
persistent identity: component2035684
display id: component2035684
version: 1
top level: BBa_K283021
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_K094100
type: Component
persistent identity: component2035692
display id: component2035692
version: 1
top level: BBa_K283021
access: public
definition: BBa_K094100

BBa_B0010
type: Component
persistent identity: component2035693
display id: component2035693
version: 1
top level: BBa_K283021
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component2035690
display id: component2035690
version: 1
top level: BBa_K283021
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component2035695
display id: component2035695
version: 1
top level: BBa_K283021
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2035693
display id: annotation2035693
version: 1
top level: BBa_K283021
location: https://synbiohub.org/public/igem/BBa_K283021/annotation2035693/range2035693/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K283021/annotation2035693/range2035693/1
type: Range
persistent identity: range2035693
display id: range2035693
version: 1
direction: direction/forward
top level: BBa_K283021
start: 784
end: 863
orientation: inline

BBa_K094100
type: SequenceAnnotation
persistent identity: annotation2035692
display id: annotation2035692
version: 1
top level: BBa_K283021
location: https://synbiohub.org/public/igem/BBa_K283021/annotation2035692/range2035692/1
component: BBa_K094100

https://synbiohub.org/public/igem/BBa_K283021/annotation2035692/range2035692/1
type: Range
persistent identity: range2035692
display id: range2035692
version: 1
direction: direction/reverse
top level: BBa_K283021
start: 81
end: 775
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2035690
display id: annotation2035690
version: 1
top level: BBa_K283021
location: https://synbiohub.org/public/igem/BBa_K283021/annotation2035690/range2035690/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K283021/annotation2035690/range2035690/1
type: Range
persistent identity: range2035690
display id: range2035690
version: 1
direction: direction/reverse
top level: BBa_K283021
start: 63
end: 74
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2035684
display id: annotation2035684
version: 1
top level: BBa_K283021
location: https://synbiohub.org/public/igem/BBa_K283021/annotation2035684/range2035684/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K283021/annotation2035684/range2035684/1
type: Range
persistent identity: range2035684
display id: range2035684
version: 1
direction: direction/forward
top level: BBa_K283021
start: 1
end: 54
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2035695
display id: annotation2035695
version: 1
top level: BBa_K283021
location: https://synbiohub.org/public/igem/BBa_K283021/annotation2035695/range2035695/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K283021/annotation2035695/range2035695/1
type: Range
persistent identity: range2035695
display id: range2035695
version: 1
direction: direction/forward
top level: BBa_K283021
start: 872
end: 912
orientation: inline

BBa_K819010
type: ComponentDefinition
persistent identity: BBa K819010
display id: BBa_K819010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Medium cheZ generator
creator: Xing Wenmin
created: 2012-09-02T11:00:00Z
modified: 2015-05-08T01:13:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1077_
m user id: 0
owner id: 13785
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: cheZ ligated to a medium promoter. This part express CheZ protein mediumly and will induce medium mobility in delta cheZ E.coli strain.
mutable notes: There is a point mutation at position 56 of CheZ coding sequence with have little effect to its function and the bases corresponding to stop codon is changed to bases coding a more frequently used stop codon.
mutable provenance: The promoter used here is from Partsregistry. The CheZ gene is from E.coli strain MG1655 by colony PCR and aligned with CheZ sequence at EcoGene database.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: CheZ
sequence annotation: BBa_J23113
sequence annotation: B0034
sequence: https://synbiohub.org/public/igem/BBa_K819010_sequence/1

CheZ
type: SequenceAnnotation
persistent identity: annotation2197375
display id: annotation2197375
version: 1
top level: BBa_K819010
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K819010/annotation2197375/range2197375/1

https://synbiohub.org/public/igem/BBa_K819010/annotation2197375/range2197375/1
type: Range
persistent identity: range2197375
display id: range2197375
version: 1
direction: direction/forward
top level: BBa_K819010
start: 62
end: 706
orientation: inline

BBa_J23113
type: SequenceAnnotation
persistent identity: annotation2197349
display id: annotation2197349
version: 1
top level: BBa_K819010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K819010/annotation2197349/range2197349/1

https://synbiohub.org/public/igem/BBa_K819010/annotation2197349/range2197349/1
type: Range
persistent identity: range2197349
display id: range2197349
version: 1
direction: direction/forward
top level: BBa_K819010
start: 1
end: 35
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2197359
display id: annotation2197359
version: 1
top level: BBa_K819010
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K819010/annotation2197359/range2197359/1

https://synbiohub.org/public/igem/BBa_K819010/annotation2197359/range2197359/1
type: Range
persistent identity: range2197359
display id: range2197359
version: 1
direction: direction/forward
top level: BBa_K819010
start: 44
end: 55
orientation: inline

BBa_K777124
type: ComponentDefinition
persistent identity: BBa K777124
display id: BBa_K777124
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: yhjH under the control of constitutive promoter J23114
creator: Team Goettingen
created: 2012-09-21T11:00:00Z
modified: 2015-05-08T01:13:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The product of this gene is a phosphodiesterase which is involved in regulating the levels of c-di-GMP, a bacterial signaling molecule. High levels of c-di-GMP promote biofilm synthesis and inhibit flagella by binding to YcgR which then acts as a "brake" on FliG and FliM. Levels of c-di-GMP are elevated and motility is reduced in ''yhjH''-mutants. Here we used 3 different constitutive [http://partsregistry.org/Part:BBa_J23100 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''yhjH'' expression affect the motility of ''E. coli''.
mutable notes: * ''yhjH'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: ** Fwd: 5'-gtttcttcgaattcgcggccgcttctagatgataaggcaggttatccagc-3' ** Rev: 5'-gtttcttcctgcagcggccgctactagtattatagcgccagaaccgccgtattcagc-3'
mutable provenance: * ''yhjH'' was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). * J23112 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23114
component: BBa_K777121
sequence annotation: BBa_J23114
sequence annotation: BBa_K777121
sequence: https://synbiohub.org/public/igem/BBa_K777124_sequence/1

BBa_J23114
type: Component
persistent identity: component2193022
display id: component2193022
version: 1
top level: BBa_K777124
access: public
definition: https://synbiohub.org/public/igem/BBa_J23114/1

BBa_K777121
type: Component
persistent identity: component2193024
display id: component2193024
version: 1
top level: BBa_K777124
access: public
definition: BBa_K777121

BBa_J23114
type: SequenceAnnotation
persistent identity: annotation2193022
display id: annotation2193022
version: 1
top level: BBa_K777124
location: https://synbiohub.org/public/igem/BBa_K777124/annotation2193022/range2193022/1
component: BBa_J23114

https://synbiohub.org/public/igem/BBa_K777124/annotation2193022/range2193022/1
type: Range
persistent identity: range2193022
display id: range2193022
version: 1
direction: direction/reverse
top level: BBa_K777124
start: 1
end: 35
orientation: inline

BBa_K777121
type: SequenceAnnotation
persistent identity: annotation2193024
display id: annotation2193024
version: 1
top level: BBa_K777124
location: https://synbiohub.org/public/igem/BBa_K777124/annotation2193024/range2193024/1
component: BBa_K777121

https://synbiohub.org/public/igem/BBa_K777124/annotation2193024/range2193024/1
type: Range
persistent identity: range2193024
display id: range2193024
version: 1
direction: direction/reverse
top level: BBa_K777124
start: 42
end: 809
orientation: inline

BBa_K777123
type: ComponentDefinition
persistent identity: BBa K777123
display id: BBa_K777123
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: yhjH under the control of constitutive promoter J23112
creator: Team Goettingen
created: 2012-09-21T11:00:00Z
modified: 2015-05-08T01:13:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The product of this gene is a phosphodiesterase which is involved in regulating the levels of c-di-GMP, a bacterial signaling molecule. High levels of c-di-GMP promote biofilm synthesis and inhibit flagella by binding to YcgR which then acts as a "brake" on FliG and FliM. Levels of c-di-GMP are elevated and motility is reduced in ''yhjH''-mutants. Here we used 3 different constitutive [http://partsregistry.org/Part:BBa_J23100 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''yhjH'' expression affect the motility of ''E. coli''.
mutable notes: * ''yhjH'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: ** Fwd: 5'-gtttcttcgaattcgcggccgcttctagatgataaggcaggttatccagc-3' ** Rev: 5'-gtttcttcctgcagcggccgctactagtattatagcgccagaaccgccgtattcagc-3'
mutable provenance: * ''yhjH'' was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). * J23100 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K777121
component: BBa_J23112
sequence annotation: BBa_K777121
sequence annotation: BBa_J23112
sequence: https://synbiohub.org/public/igem/BBa_K777123_sequence/1

BBa_K777121
type: Component
persistent identity: component2192774
display id: component2192774
version: 1
top level: BBa_K777123
access: public
definition: BBa_K777121

BBa_J23112
type: Component
persistent identity: component2192772
display id: component2192772
version: 1
top level: BBa_K777123
access: public
definition: https://synbiohub.org/public/igem/BBa_J23112/1

BBa_K777121
type: SequenceAnnotation
persistent identity: annotation2192774
display id: annotation2192774
version: 1
top level: BBa_K777123
location: https://synbiohub.org/public/igem/BBa_K777123/annotation2192774/range2192774/1
component: BBa_K777121

https://synbiohub.org/public/igem/BBa_K777123/annotation2192774/range2192774/1
type: Range
persistent identity: range2192774
display id: range2192774
version: 1
direction: direction/reverse
top level: BBa_K777123
start: 42
end: 809
orientation: inline

BBa_J23112
type: SequenceAnnotation
persistent identity: annotation2192772
display id: annotation2192772
version: 1
top level: BBa_K777123
location: https://synbiohub.org/public/igem/BBa_K777123/annotation2192772/range2192772/1
component: BBa_J23112

https://synbiohub.org/public/igem/BBa_K777123/annotation2192772/range2192772/1
type: Range
persistent identity: range2192772
display id: range2192772
version: 1
direction: direction/reverse
top level: BBa_K777123
start: 1
end: 35
orientation: inline

BBa_K108012
type: ComponentDefinition
persistent identity: BBa K108012
display id: BBa_K108012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MotA
creator: Qi Liu
created: 2008-10-22T11:00:00Z
modified: 2015-05-08T01:09:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _222_
m user id: 0
owner id: 2671
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: proton conductor component of flagella motor
mutable notes: PCR using primers with biobrick prefix and surfix, and then cloned into pSB1AC3.
mutable provenance: E.coli K12 DH10B genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: MotA
sequence: https://synbiohub.org/public/igem/BBa_K108012_sequence/1

MotA
type: SequenceAnnotation
persistent identity: annotation1986957
display id: annotation1986957
version: 1
top level: BBa_K108012
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K108012/annotation1986957/range1986957/1

https://synbiohub.org/public/igem/BBa_K108012/annotation1986957/range1986957/1
type: Range
persistent identity: range1986957
display id: range1986957
version: 1
direction: direction/forward
top level: BBa_K108012
start: 1
end: 885
orientation: inline

aspA
type: ComponentDefinition
persistent identity: BBa C0083
display id: BBa_C0083
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: aspartate ammonia-lyase
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Coding sequence for AspA enzyme. AspA aminates fumarate to make aspartate. Aspartate can be used as a bacterial chemotaxis signal.
mutable notes: The start codon was changed from GTG to ATG to match BioBricks standards. Five silent mutations were introduced to eliminate EcoRI and PstI restriction enzyme sites. EcoRI sites were eliminated at bases 588 and 657 with C -> T mutations. PstI sites were eliminated at bases 624, 999, and 1125 with G -> A mutations. The mutations were designed to match codons with similar frequencies of usage in E. coli.
mutable provenance: AE000486 E. coli K12
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: AspA
sequence annotation: Help:Barcodes
sequence annotation: G
sequence annotation: C
sequence annotation: LVA
sequence: https://synbiohub.org/public/igem/BBa_C0083_sequence/1

AspA
type: SequenceAnnotation
persistent identity: annotation300981
display id: annotation300981
version: 1
top level: aspA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0083/annotation300981/range300981/1

https://synbiohub.org/public/igem/BBa_C0083/annotation300981/range300981/1
type: Range
persistent identity: range300981
display id: range300981
version: 1
direction: direction/forward
top level: aspA
start: 1
end: 1479
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213998
display id: annotation2213998
version: 1
top level: aspA
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0083/annotation2213998/range2213998/1

https://synbiohub.org/public/igem/BBa_C0083/annotation2213998/range2213998/1
type: Range
persistent identity: range2213998
display id: range2213998
version: 1
direction: direction/forward
top level: aspA
start: 1519
end: 1543
orientation: inline

G
type: SequenceAnnotation
persistent identity: annotation300982
display id: annotation300982
version: 1
top level: aspA
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_C0083/annotation300982/range300982/1

https://synbiohub.org/public/igem/BBa_C0083/annotation300982/range300982/1
type: Range
persistent identity: range300982
display id: range300982
version: 1
direction: direction/forward
top level: aspA
start: 1
end: 1
orientation: inline

G
type: SequenceAnnotation
persistent identity: annotation300987
display id: annotation300987
version: 1
top level: aspA
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_C0083/annotation300987/range300987/1

https://synbiohub.org/public/igem/BBa_C0083/annotation300987/range300987/1
type: Range
persistent identity: range300987
display id: range300987
version: 1
direction: direction/forward
top level: aspA
start: 999
end: 999
orientation: inline

G
type: SequenceAnnotation
persistent identity: annotation300984
display id: annotation300984
version: 1
top level: aspA
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_C0083/annotation300984/range300984/1

https://synbiohub.org/public/igem/BBa_C0083/annotation300984/range300984/1
type: Range
persistent identity: range300984
display id: range300984
version: 1
direction: direction/forward
top level: aspA
start: 624
end: 624
orientation: inline

G
type: SequenceAnnotation
persistent identity: annotation300988
display id: annotation300988
version: 1
top level: aspA
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_C0083/annotation300988/range300988/1

https://synbiohub.org/public/igem/BBa_C0083/annotation300988/range300988/1
type: Range
persistent identity: range300988
display id: range300988
version: 1
direction: direction/forward
top level: aspA
start: 1125
end: 1125
orientation: inline

C
type: SequenceAnnotation
persistent identity: annotation300983
display id: annotation300983
version: 1
top level: aspA
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_C0083/annotation300983/range300983/1

https://synbiohub.org/public/igem/BBa_C0083/annotation300983/range300983/1
type: Range
persistent identity: range300983
display id: range300983
version: 1
direction: direction/forward
top level: aspA
start: 588
end: 588
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation300993
display id: annotation300993
version: 1
top level: aspA
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0083/annotation300993/range300993/1

https://synbiohub.org/public/igem/BBa_C0083/annotation300993/range300993/1
type: Range
persistent identity: range300993
display id: range300993
version: 1
direction: direction/forward
top level: aspA
start: 1480
end: 1512
orientation: inline

C
type: SequenceAnnotation
persistent identity: annotation300986
display id: annotation300986
version: 1
top level: aspA
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_C0083/annotation300986/range300986/1

https://synbiohub.org/public/igem/BBa_C0083/annotation300986/range300986/1
type: Range
persistent identity: range300986
display id: range300986
version: 1
direction: direction/forward
top level: aspA
start: 657
end: 657
orientation: inline

BBa_K518005
type: ComponentDefinition
persistent identity: BBa K518005
display id: BBa_K518005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cheZ
creator: Masato Ohgishi
created: 2011-09-14T11:00:00Z
modified: 2015-05-08T01:12:33Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _683_
m user id: 0
owner id: 8305
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: CheZ is responsible for the dephosphorylation of flagellum-regulating protein CheY. It has been reported that cheZ-/- strain has a higher frequency of direction change and thus a narrower range of mobility.
mutable notes: None.
mutable provenance: Cloned from Escherichia coli JM109 strain.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K518005_sequence/1

BBa_K094100
type: ComponentDefinition
persistent identity: BBa K094100
display id: BBa_K094100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cheZ gene
creator: Liu Chenli
created: 2008-10-12T11:00:00Z
modified: 2015-05-08T01:08:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _255_
m user id: 0
owner id: 2589
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part contains the cheZ operon coding region without an rbs nor a terminator. cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement.
mutable notes: This part is without promoter, rbs or terminator.
mutable provenance: The origin of the cheZ gene comes from E. Coli strain MG1655 chromosome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cheZ
sequence: https://synbiohub.org/public/igem/BBa_K094100_sequence/1

cheZ
type: SequenceAnnotation
persistent identity: annotation1980478
display id: annotation1980478
version: 1
top level: BBa_K094100
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K094100/annotation1980478/range1980478/1

https://synbiohub.org/public/igem/BBa_K094100/annotation1980478/range1980478/1
type: Range
persistent identity: range1980478
display id: range1980478
version: 1
direction: direction/forward
top level: BBa_K094100
start: 1
end: 645
orientation: inline

BBa_K147000
type: ComponentDefinition
persistent identity: BBa K147000
display id: BBa_K147000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cheZ
creator: Roxanne Shank
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:10:38Z
discontinued: false
dominant: false
group u list: _242_
m user id: 0
owner id: 3171
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The cheZ motility protein is a protein kinase which dephosphorylates cheY. cheY regulates the clockwise and counterclockwise rotation of the motor flagella. If cheY is phosphorylated the motor flagella are not in sync and the bacterium tumbles. If cheY is not phosphorylated the motor flagella are in sync and the bacterium runs (often towards a chemoattractant)
mutable notes: Nothing of note
mutable provenance: Amplified from pTopp plasmid.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K147000_sequence/1

BBa_K283019
type: ComponentDefinition
persistent identity: BBa K283019
display id: BBa_K283019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FF_FF ansB promoter-rbs- GFP (LVA)- terminators
creator: Liu Chenli
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _383_
m user id: 0
owner id: 5494
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part consist of a strong rbs and the cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement and is tagged by a 6-Histidine tail. This chez coding region do not contain any terminator
mutable notes: BBa_K283019
mutable provenance: The chez-Histag coding region is self-designed The rbs, double terminator are from the registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K283031
component: BBa_J04031
component: BBa_B0034
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_J04031
sequence annotation: BBa_K283031
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K283019_sequence/1

BBa_K283031
type: Component
persistent identity: component2056594
display id: component2056594
version: 1
top level: BBa_K283019
access: public
definition: https://synbiohub.org/public/igem/BBa_K283031/1

BBa_J04031
type: Component
persistent identity: component2056601
display id: component2056601
version: 1
top level: BBa_K283019
access: public
definition: https://synbiohub.org/public/igem/BBa_J04031/1

BBa_B0034
type: Component
persistent identity: component2056596
display id: component2056596
version: 1
top level: BBa_K283019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component2056604
display id: component2056604
version: 1
top level: BBa_K283019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component2056602
display id: component2056602
version: 1
top level: BBa_K283019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2056596
display id: annotation2056596
version: 1
top level: BBa_K283019
location: https://synbiohub.org/public/igem/BBa_K283019/annotation2056596/range2056596/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K283019/annotation2056596/range2056596/1
type: Range
persistent identity: range2056596
display id: range2056596
version: 1
direction: direction/reverse
top level: BBa_K283019
start: 114
end: 125
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2056602
display id: annotation2056602
version: 1
top level: BBa_K283019
location: https://synbiohub.org/public/igem/BBa_K283019/annotation2056602/range2056602/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K283019/annotation2056602/range2056602/1
type: Range
persistent identity: range2056602
display id: range2056602
version: 1
direction: direction/forward
top level: BBa_K283019
start: 899
end: 978
orientation: inline

BBa_J04031
type: SequenceAnnotation
persistent identity: annotation2056601
display id: annotation2056601
version: 1
top level: BBa_K283019
location: https://synbiohub.org/public/igem/BBa_K283019/annotation2056601/range2056601/1
component: BBa_J04031

https://synbiohub.org/public/igem/BBa_K283019/annotation2056601/range2056601/1
type: Range
persistent identity: range2056601
display id: range2056601
version: 1
direction: direction/reverse
top level: BBa_K283019
start: 132
end: 890
orientation: inline

BBa_K283031
type: SequenceAnnotation
persistent identity: annotation2056594
display id: annotation2056594
version: 1
top level: BBa_K283019
location: https://synbiohub.org/public/igem/BBa_K283019/annotation2056594/range2056594/1
component: BBa_K283031

https://synbiohub.org/public/igem/BBa_K283019/annotation2056594/range2056594/1
type: Range
persistent identity: range2056594
display id: range2056594
version: 1
direction: direction/reverse
top level: BBa_K283019
start: 1
end: 105
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2056604
display id: annotation2056604
version: 1
top level: BBa_K283019
location: https://synbiohub.org/public/igem/BBa_K283019/annotation2056604/range2056604/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K283019/annotation2056604/range2056604/1
type: Range
persistent identity: range2056604
display id: range2056604
version: 1
direction: direction/forward
top level: BBa_K283019
start: 987
end: 1027
orientation: inline

BBa_K108006
type: ComponentDefinition
persistent identity: BBa K108006
display id: BBa_K108006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FlgI
creator: Qi Liu
created: 2008-10-22T11:00:00Z
modified: 2015-05-08T01:09:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _222_
m user id: 0
owner id: 2671
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: flagellar basal body protein
mutable notes: PCR using primers with biobrick prefix and surfix, and then cloned into pSB1AC3.
mutable provenance: E.coli K12 DH10B genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: FlgI
sequence: https://synbiohub.org/public/igem/BBa_K108006_sequence/1

FlgI
type: SequenceAnnotation
persistent identity: annotation1986951
display id: annotation1986951
version: 1
top level: BBa_K108006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K108006/annotation1986951/range1986951/1

https://synbiohub.org/public/igem/BBa_K108006/annotation1986951/range1986951/1
type: Range
persistent identity: range1986951
display id: range1986951
version: 1
direction: direction/forward
top level: BBa_K108006
start: 1
end: 1095
orientation: inline

CheZ-GFP
type: ComponentDefinition
persistent identity: BBa K1709002
display id: BBa_K1709002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CheZ-GFP
creator: Astrid Deryckere
created: 2015-09-09T11:00:00Z
modified: 2015-09-18T09:28:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2129_
m user id: 25389
owner id: 25389
owning group id: 9
status: status/Planning
bookmark: true
mutable description: Upon recognition of a small molecule (e.g. nutrients or toxins), chemoreceptors transduce a signal through a set of Che proteins. This group of methylesterases and phosphatases regulates the rotation of the flagella. At the end of the chain of Che proteins is CheY. In phosphorylated state, CheY binds the flagella and causes the cells to tumble. The phosphatase CheZ dephosphorylates CheY, inducing dissociation of CheY from the flagella enabling the cells to swim. Cells lacking the CheZ protein are unable to swim and will tumble excessively and incessantly.
mutable notes: 1 bp in BBa_K629003 was adapted to create an original restriciton site in the plasmid. CheZ was fused to GFP (BBa_K082003) and its LVA tag was codon-optimized to avoid introducing artificial DNA repetitions.
mutable provenance: DNA sequences originate from the E. Coli genome and previous biobrick sequences.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Ochre Stop Codon
sequence annotation: CheZ Coding Region
sequence annotation: GFP Coding Region
sequence annotation: LVA-tag
sequence annotation: ATG Start Codon
sequence: https://synbiohub.org/public/igem/BBa_K1709002_sequence/1

Ochre Stop Codon
type: SequenceAnnotation
persistent identity: annotation2448531
display id: annotation2448531
version: 1
top level: CheZ-GFP
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1709002/annotation2448531/range2448531/1

https://synbiohub.org/public/igem/BBa_K1709002/annotation2448531/range2448531/1
type: Range
persistent identity: range2448531
display id: range2448531
version: 1
direction: direction/forward
top level: CheZ-GFP
start: 1399
end: 1401
orientation: inline

CheZ Coding Region
type: SequenceAnnotation
persistent identity: annotation2448532
display id: annotation2448532
version: 1
top level: CheZ-GFP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1709002/annotation2448532/range2448532/1

https://synbiohub.org/public/igem/BBa_K1709002/annotation2448532/range2448532/1
type: Range
persistent identity: range2448532
display id: range2448532
version: 1
direction: direction/forward
top level: CheZ-GFP
start: 1
end: 642
orientation: inline

GFP Coding Region
type: SequenceAnnotation
persistent identity: annotation2448533
display id: annotation2448533
version: 1
top level: CheZ-GFP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1709002/annotation2448533/range2448533/1

https://synbiohub.org/public/igem/BBa_K1709002/annotation2448533/range2448533/1
type: Range
persistent identity: range2448533
display id: range2448533
version: 1
direction: direction/forward
top level: CheZ-GFP
start: 646
end: 1365
orientation: inline

LVA-tag
type: SequenceAnnotation
persistent identity: annotation2448534
display id: annotation2448534
version: 1
top level: CheZ-GFP
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1709002/annotation2448534/range2448534/1

https://synbiohub.org/public/igem/BBa_K1709002/annotation2448534/range2448534/1
type: Range
persistent identity: range2448534
display id: range2448534
version: 1
direction: direction/forward
top level: CheZ-GFP
start: 1366
end: 1401
orientation: inline

ATG Start Codon
type: SequenceAnnotation
persistent identity: annotation2448530
display id: annotation2448530
version: 1
top level: CheZ-GFP
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1709002/annotation2448530/range2448530/1

https://synbiohub.org/public/igem/BBa_K1709002/annotation2448530/range2448530/1
type: Range
persistent identity: range2448530
display id: range2448530
version: 1
direction: direction/forward
top level: CheZ-GFP
start: 1
end: 3
orientation: inline

BBa_K777102
type: ComponentDefinition
persistent identity: BBa K777102
display id: BBa_K777102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: flhDC operon under the control of constitutive promoter J23104
creator: Team Goettingen
created: 2012-09-18T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The flhDC operon is the master regulator of motility and chemotaxis in E. coli. FlhDC forms heterotetramers and activates class II operons in concert with sigma factor 70. Among the gene products of class II operons are several components of the flagellum and the alternative sigma factor FliA which is essential for the transcription of class III genes. Here we used a set of 8 constitutive Anderson-promoters from the 2006 Berkeley group to test how different levels of constitutive flhDC expression affect the motility of E. coli.
mutable notes: * The PstI restriction site in the flhDC sequence was mutated to CTGCGG using the reverse primer * flhDC was amplified from genomic DNA of E. coli DH10B via PCR using the following primers: ** Fwd: 5'-actgaattcgcggccgcttctagatgcatacctccgagttgctg-3' ** Rev: 5'-tcctgcagcggccgctactagttactgcccgggatggcggttgacataagcCgcaggcaaagctgccaacag-3' (the capitalized "C" induces the mutation for removal of the PstI site)
mutable provenance: * The part was amplified from genomic DNA of E. coli str. K-12 substr. DH10B, complete genome (CP000948.1). * J23100 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K777100
component: BBa_J23104
sequence annotation: BBa_K777100
sequence annotation: BBa_J23104
sequence: https://synbiohub.org/public/igem/BBa_K777102_sequence/1

BBa_K777100
type: Component
persistent identity: component2187014
display id: component2187014
version: 1
top level: BBa_K777102
access: public
definition: BBa_K777100

BBa_J23104
type: Component
persistent identity: component2187006
display id: component2187006
version: 1
top level: BBa_K777102
access: public
definition: https://synbiohub.org/public/igem/BBa_J23104/1

BBa_K777100
type: SequenceAnnotation
persistent identity: annotation2187014
display id: annotation2187014
version: 1
top level: BBa_K777102
location: https://synbiohub.org/public/igem/BBa_K777102/annotation2187014/range2187014/1
component: BBa_K777100

https://synbiohub.org/public/igem/BBa_K777102/annotation2187014/range2187014/1
type: Range
persistent identity: range2187014
display id: range2187014
version: 1
direction: direction/reverse
top level: BBa_K777102
start: 42
end: 910
orientation: inline

BBa_J23104
type: SequenceAnnotation
persistent identity: annotation2187006
display id: annotation2187006
version: 1
top level: BBa_K777102
location: https://synbiohub.org/public/igem/BBa_K777102/annotation2187006/range2187006/1
component: BBa_J23104

https://synbiohub.org/public/igem/BBa_K777102/annotation2187006/range2187006/1
type: Range
persistent identity: range2187006
display id: range2187006
version: 1
direction: direction/reverse
top level: BBa_K777102
start: 1
end: 35
orientation: inline

BBa_K1709003
type: ComponentDefinition
persistent identity: BBa K1709003
display id: BBa_K1709003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CheZ-GFP
creator: Astrid Deryckere
created: 2015-09-15T11:00:00Z
modified: 2015-09-26T08:47:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2129_
m user id: 25389
owner id: 25389
owning group id: 9
status: status/Planning
bookmark: true
mutable description: Upon recognition of a small molecule (e.g. nutrients or toxins), chemoreceptors transduce a signal through a set of Che proteins. This group of methylesterases and phosphatases regulates the rotation of the flagella. At the end of the chain of Che proteins is CheY. In phosphorylated state, CheY binds the flagella and causes the cells to tumble. The phosphatase CheZ dephosphorylates CheY, inducing dissociation of CheY from the flagella enabling the cells to swim. Cells lacking the CheZ protein are unable to swim and will tumble excessively and incessantly.
mutable notes: 1 bp in BBa_K629003 was adapted to create an original restriciton site in the plasmid. CheZ was fused to GFP (BBa_K082003) and its LVA tag was codon-optimized to avoid introducing artificial DNA repetitions.
mutable provenance: DNA sequences originate from the E. Coli genome and previous biobrick sequences. The ribosome binding site was chosen from the Anderson RBS family.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1709002
component: BBa_J61101
sequence annotation: BBa_J61101
sequence annotation: BBa_K1709002
sequence: https://synbiohub.org/public/igem/BBa_K1709003_sequence/1

BBa_K1709002
type: Component
persistent identity: component2472552
display id: component2472552
version: 1
top level: BBa_K1709003
access: public
definition: CheZ-GFP

BBa_J61101
type: Component
persistent identity: component2472546
display id: component2472546
version: 1
top level: BBa_K1709003
access: public
definition: https://synbiohub.org/public/igem/BBa_J61101/1

BBa_J61101
type: SequenceAnnotation
persistent identity: annotation2472546
display id: annotation2472546
version: 1
top level: BBa_K1709003
location: https://synbiohub.org/public/igem/BBa_K1709003/annotation2472546/range2472546/1
component: BBa_J61101

https://synbiohub.org/public/igem/BBa_K1709003/annotation2472546/range2472546/1
type: Range
persistent identity: range2472546
display id: range2472546
version: 1
direction: direction/reverse
top level: BBa_K1709003
start: 1
end: 12
orientation: inline

BBa_K1709002
type: SequenceAnnotation
persistent identity: annotation2472552
display id: annotation2472552
version: 1
top level: BBa_K1709003
location: https://synbiohub.org/public/igem/BBa_K1709003/annotation2472552/range2472552/1
component: BBa_K1709002

https://synbiohub.org/public/igem/BBa_K1709003/annotation2472552/range2472552/1
type: Range
persistent identity: range2472552
display id: range2472552
version: 1
direction: direction/reverse
top level: BBa_K1709003
start: 19
end: 1419
orientation: inline

BBa_K283008
type: ComponentDefinition
persistent identity: BBa K283008
display id: BBa_K283008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: chez_Histag
creator: W Ling , Nelson Chu, Shi Lei
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _383_
m user id: 0
owner id: 5494
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part consists of a chez coding region followed by a 6-Histidine tail
mutable notes: BBa_K283008
mutable provenance: self-designed
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: 6-His tag
sequence annotation: BBa_K094100
sequence annotation: cheZ
sequence: https://synbiohub.org/public/igem/BBa_K283008_sequence/1

6-His tag
type: SequenceAnnotation
persistent identity: annotation2035366
display id: annotation2035366
version: 1
top level: BBa_K283008
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K283008/annotation2035366/range2035366/1

https://synbiohub.org/public/igem/BBa_K283008/annotation2035366/range2035366/1
type: Range
persistent identity: range2035366
display id: range2035366
version: 1
direction: direction/reverse
top level: BBa_K283008
start: 696
end: 713
orientation: inline

BBa_K094100
type: SequenceAnnotation
persistent identity: annotation2035290
display id: annotation2035290
version: 1
top level: BBa_K283008
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K283008/annotation2035290/range2035290/1

https://synbiohub.org/public/igem/BBa_K283008/annotation2035290/range2035290/1
type: Range
persistent identity: range2035290
display id: range2035290
version: 1
direction: direction/reverse
top level: BBa_K283008
start: 1
end: 695
orientation: inline

cheZ
type: SequenceAnnotation
persistent identity: annotation2035289
display id: annotation2035289
version: 1
top level: BBa_K283008
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K283008/annotation2035289/range2035289/1

https://synbiohub.org/public/igem/BBa_K283008/annotation2035289/range2035289/1
type: Range
persistent identity: range2035289
display id: range2035289
version: 1
direction: direction/forward
top level: BBa_K283008
start: 1
end: 645
orientation: inline

BBa_K777100
type: ComponentDefinition
persistent identity: BBa K777100
display id: BBa_K777100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: flhDC
creator: Team Goettingen
created: 2012-09-14T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The flhDC operon is the master regulator of motility and chemotaxis in E. coli. FlhDC forms heterotetramers and activates class II operons in concert with sigma factor 70. Among the gene products of class II operons are several components of the flagellum and the alternative sigma factor FliA which is essential for the transcription of class III genes.
mutable notes: The PstI restriction site was mutated to CTGCGG using the reverse primer.
mutable provenance: The part was amplified from genomic DNA of E. coli str. K-12 substr. DH10B, complete genome (CP000948.1).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: PstI site removed
sequence annotation: FlhC coding region
sequence annotation: TAA stop codon
sequence annotation: TGA stop codon
sequence annotation: FlhD coding region
sequence annotation: ATG start codon
sequence: https://synbiohub.org/public/igem/BBa_K777100_sequence/1

PstI site removed
type: SequenceAnnotation
persistent identity: annotation2183225
display id: annotation2183225
version: 1
top level: BBa_K777100
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K777100/annotation2183225/range2183225/1

https://synbiohub.org/public/igem/BBa_K777100/annotation2183225/range2183225/1
type: Range
persistent identity: range2183225
display id: range2183225
version: 1
direction: direction/forward
top level: BBa_K777100
start: 839
end: 839
orientation: inline

FlhC coding region
type: SequenceAnnotation
persistent identity: annotation2183222
display id: annotation2183222
version: 1
top level: BBa_K777100
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K777100/annotation2183222/range2183222/1

https://synbiohub.org/public/igem/BBa_K777100/annotation2183222/range2183222/1
type: Range
persistent identity: range2183222
display id: range2183222
version: 1
direction: direction/forward
top level: BBa_K777100
start: 354
end: 869
orientation: inline

TAA stop codon
type: SequenceAnnotation
persistent identity: annotation2183224
display id: annotation2183224
version: 1
top level: BBa_K777100
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K777100/annotation2183224/range2183224/1

https://synbiohub.org/public/igem/BBa_K777100/annotation2183224/range2183224/1
type: Range
persistent identity: range2183224
display id: range2183224
version: 1
direction: direction/forward
top level: BBa_K777100
start: 867
end: 869
orientation: inline

TGA stop codon
type: SequenceAnnotation
persistent identity: annotation2183218
display id: annotation2183218
version: 1
top level: BBa_K777100
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K777100/annotation2183218/range2183218/1

https://synbiohub.org/public/igem/BBa_K777100/annotation2183218/range2183218/1
type: Range
persistent identity: range2183218
display id: range2183218
version: 1
direction: direction/forward
top level: BBa_K777100
start: 349
end: 351
orientation: inline

FlhD coding region
type: SequenceAnnotation
persistent identity: annotation2183217
display id: annotation2183217
version: 1
top level: BBa_K777100
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K777100/annotation2183217/range2183217/1

https://synbiohub.org/public/igem/BBa_K777100/annotation2183217/range2183217/1
type: Range
persistent identity: range2183217
display id: range2183217
version: 1
direction: direction/forward
top level: BBa_K777100
start: 1
end: 351
orientation: inline

ATG start codon
type: SequenceAnnotation
persistent identity: annotation2183216
display id: annotation2183216
version: 1
top level: BBa_K777100
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K777100/annotation2183216/range2183216/1

https://synbiohub.org/public/igem/BBa_K777100/annotation2183216/range2183216/1
type: Range
persistent identity: range2183216
display id: range2183216
version: 1
direction: direction/forward
top level: BBa_K777100
start: 1
end: 3
orientation: inline

ATG start codon
type: SequenceAnnotation
persistent identity: annotation2183220
display id: annotation2183220
version: 1
top level: BBa_K777100
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K777100/annotation2183220/range2183220/1

https://synbiohub.org/public/igem/BBa_K777100/annotation2183220/range2183220/1
type: Range
persistent identity: range2183220
display id: range2183220
version: 1
direction: direction/forward
top level: BBa_K777100
start: 354
end: 356
orientation: inline

BBa_K094103
type: ComponentDefinition
persistent identity: BBa K094103
display id: BBa_K094103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: plux-rbs-cheZ-terminator
creator: Liu Chenli
created: 2008-10-13T11:00:00Z
modified: 2015-05-08T01:08:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _255_
m user id: 0
owner id: 2589
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a cheZ gene that can be induced by luxR protein under high AHL concentration. cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement.
mutable notes: N/A
mutable provenance: composited from E. Coli cheZ gene and lux promoter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_B0034
component: BBa_K094013
component: BBa_R0062
component: BBa_K094100
sequence annotation: BBa_R0062
sequence annotation: BBa_K094100
sequence annotation: BBa_B0034
sequence annotation: BBa_K094013
sequence: https://synbiohub.org/public/igem/BBa_K094103_sequence/1

BBa_B0034
type: Component
persistent identity: component1990872
display id: component1990872
version: 1
top level: BBa_K094103
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K094013
type: Component
persistent identity: component1990875
display id: component1990875
version: 1
top level: BBa_K094103
access: public
definition: https://synbiohub.org/public/igem/BBa_K094013/1

BBa_R0062
type: Component
persistent identity: component1990867
display id: component1990867
version: 1
top level: BBa_K094103
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_K094100
type: Component
persistent identity: component1990874
display id: component1990874
version: 1
top level: BBa_K094103
access: public
definition: BBa_K094100

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation1990867
display id: annotation1990867
version: 1
top level: BBa_K094103
location: https://synbiohub.org/public/igem/BBa_K094103/annotation1990867/range1990867/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K094103/annotation1990867/range1990867/1
type: Range
persistent identity: range1990867
display id: range1990867
version: 1
direction: direction/forward
top level: BBa_K094103
start: 1
end: 55
orientation: inline

BBa_K094100
type: SequenceAnnotation
persistent identity: annotation1990874
display id: annotation1990874
version: 1
top level: BBa_K094103
location: https://synbiohub.org/public/igem/BBa_K094103/annotation1990874/range1990874/1
component: BBa_K094100

https://synbiohub.org/public/igem/BBa_K094103/annotation1990874/range1990874/1
type: Range
persistent identity: range1990874
display id: range1990874
version: 1
direction: direction/reverse
top level: BBa_K094103
start: 82
end: 776
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1990872
display id: annotation1990872
version: 1
top level: BBa_K094103
location: https://synbiohub.org/public/igem/BBa_K094103/annotation1990872/range1990872/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K094103/annotation1990872/range1990872/1
type: Range
persistent identity: range1990872
display id: range1990872
version: 1
direction: direction/reverse
top level: BBa_K094103
start: 64
end: 75
orientation: inline

BBa_K094013
type: SequenceAnnotation
persistent identity: annotation1990875
display id: annotation1990875
version: 1
top level: BBa_K094103
location: https://synbiohub.org/public/igem/BBa_K094103/annotation1990875/range1990875/1
component: BBa_K094013

https://synbiohub.org/public/igem/BBa_K094103/annotation1990875/range1990875/1
type: Range
persistent identity: range1990875
display id: range1990875
version: 1
direction: direction/reverse
top level: BBa_K094103
start: 785
end: 878
orientation: inline

BBa_K108007
type: ComponentDefinition
persistent identity: BBa K108007
display id: BBa_K108007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FliD
creator: Qi Liu
created: 2008-10-22T11:00:00Z
modified: 2015-05-08T01:09:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _222_
m user id: 0
owner id: 2671
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: flagellar filament capping protein
mutable notes: PCR using primers with biobrick prefix and surfix, and then cloned into pSB1AC3.
mutable provenance: E.coli K12 DH10B genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: FliD
sequence: https://synbiohub.org/public/igem/BBa_K108007_sequence/1

FliD
type: SequenceAnnotation
persistent identity: annotation1986952
display id: annotation1986952
version: 1
top level: BBa_K108007
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K108007/annotation1986952/range1986952/1

https://synbiohub.org/public/igem/BBa_K108007/annotation1986952/range1986952/1
type: Range
persistent identity: range1986952
display id: range1986952
version: 1
direction: direction/forward
top level: BBa_K108007
start: 1
end: 1404
orientation: inline

BBa_K094110
type: ComponentDefinition
persistent identity: BBa K094110
display id: BBa_K094110
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cheY* gene
creator: Liu Chenli
created: 2008-10-12T11:00:00Z
modified: 2015-05-08T01:08:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _255_
m user id: 0
owner id: 2589
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is the gene coding for cheY protein which controls flagella movement. This sequence, however, is mutated to give a mutant cheY protein which will not respond to the presence of cheZ protein. This part contains the coding region only.
mutable notes: This part contains only the coding region, it is without promoter, rbs and terminator.
mutable provenance: This gene is adapted from E. Coli strain MG1655 with two point mutations (D13K Y106W)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: misc
sequence annotation: cheY*
sequence: https://synbiohub.org/public/igem/BBa_K094110_sequence/1

misc
type: SequenceAnnotation
persistent identity: annotation1985292
display id: annotation1985292
version: 1
top level: BBa_K094110
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K094110/annotation1985292/range1985292/1

https://synbiohub.org/public/igem/BBa_K094110/annotation1985292/range1985292/1
type: Range
persistent identity: range1985292
display id: range1985292
version: 1
direction: direction/forward
top level: BBa_K094110
start: 1
end: 390
orientation: inline

cheY*
type: SequenceAnnotation
persistent identity: annotation1980499
display id: annotation1980499
version: 1
top level: BBa_K094110
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K094110/annotation1980499/range1980499/1

https://synbiohub.org/public/igem/BBa_K094110/annotation1980499/range1980499/1
type: Range
persistent identity: range1980499
display id: range1980499
version: 1
direction: direction/forward
top level: BBa_K094110
start: 1
end: 390
orientation: inline

BBa_K108013
type: ComponentDefinition
persistent identity: BBa K108013
display id: BBa_K108013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: YcgR
creator: Qi Liu
created: 2008-10-22T11:00:00Z
modified: 2015-05-08T01:09:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _222_
m user id: 0
owner id: 2671
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: protein involved in flagellar function
mutable notes: PCR using primers with biobrick prefix and surfix, and then cloned into pSB1AC3.
mutable provenance: E.coli K12 DH10B genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: YcgR
sequence: https://synbiohub.org/public/igem/BBa_K108013_sequence/1

YcgR
type: SequenceAnnotation
persistent identity: annotation1986958
display id: annotation1986958
version: 1
top level: BBa_K108013
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K108013/annotation1986958/range1986958/1

https://synbiohub.org/public/igem/BBa_K108013/annotation1986958/range1986958/1
type: Range
persistent identity: range1986958
display id: range1986958
version: 1
direction: direction/forward
top level: BBa_K108013
start: 1
end: 732
orientation: inline

cheZ
type: ComponentDefinition
persistent identity: BBa K629003
display id: BBa_K629003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cheZ, chemotaxis regulator, protein phosphatase for CheY
creator: Zilong WANG, Yi ZHENG
created: 2011-10-02T11:00:00Z
modified: 2015-05-08T01:12:56Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _801_
m user id: 0
owner id: 9022
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Polar localization is dependent on CheA-short. CheZ is a cytosolic phosphatase which functions in the chemotaxis signal transduction complex. Plays an important role in bacterial chemotaxis signal transduction pathway by accelerating the dephosphorylation of phosphorylated CheY (CheY-P).
mutable notes: how to control the proper expression of cheZ
mutable provenance: genome of E. Coli BL21 plys strain
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: fwd primer
sequence annotation: rev primer
sequence annotation: terminator
sequence annotation: TGA to TAA
sequence annotation: CheZ
sequence: https://synbiohub.org/public/igem/BBa_K629003_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2154422
display id: annotation2154422
version: 1
top level: cheZ
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K629003/annotation2154422/range2154422/1

https://synbiohub.org/public/igem/BBa_K629003/annotation2154422/range2154422/1
type: Range
persistent identity: range2154422
display id: range2154422
version: 1
direction: direction/forward
top level: cheZ
start: 1
end: 3
orientation: inline

fwd primer
type: SequenceAnnotation
persistent identity: annotation2154421
display id: annotation2154421
version: 1
top level: cheZ
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K629003/annotation2154421/range2154421/1

https://synbiohub.org/public/igem/BBa_K629003/annotation2154421/range2154421/1
type: Range
persistent identity: range2154421
display id: range2154421
version: 1
direction: direction/forward
top level: cheZ
start: 1
end: 20
orientation: inline

rev primer
type: SequenceAnnotation
persistent identity: annotation2154423
display id: annotation2154423
version: 1
top level: cheZ
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K629003/annotation2154423/range2154423/1

https://synbiohub.org/public/igem/BBa_K629003/annotation2154423/range2154423/1
type: Range
persistent identity: range2154423
display id: range2154423
version: 1
direction: direction/forward
top level: cheZ
start: 624
end: 645
orientation: inline

terminator
type: SequenceAnnotation
persistent identity: annotation2154424
display id: annotation2154424
version: 1
top level: cheZ
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K629003/annotation2154424/range2154424/1

https://synbiohub.org/public/igem/BBa_K629003/annotation2154424/range2154424/1
type: Range
persistent identity: range2154424
display id: range2154424
version: 1
direction: direction/forward
top level: cheZ
start: 643
end: 645
orientation: inline

TGA to TAA
type: SequenceAnnotation
persistent identity: annotation2155668
display id: annotation2155668
version: 1
top level: cheZ
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K629003/annotation2155668/range2155668/1

https://synbiohub.org/public/igem/BBa_K629003/annotation2155668/range2155668/1
type: Range
persistent identity: range2155668
display id: range2155668
version: 1
direction: direction/forward
top level: cheZ
start: 643
end: 645
orientation: inline

CheZ
type: SequenceAnnotation
persistent identity: annotation2154425
display id: annotation2154425
version: 1
top level: cheZ
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K629003/annotation2154425/range2154425/1

https://synbiohub.org/public/igem/BBa_K629003/annotation2154425/range2154425/1
type: Range
persistent identity: range2154425
display id: range2154425
version: 1
direction: direction/forward
top level: cheZ
start: 1
end: 645
orientation: inline

BBa_K1631020
type: ComponentDefinition
persistent identity: BBa K1631020
display id: BBa_K1631020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CheZ generator (under constitutive promoter BBa_J23102)
creator: Yuto Yamanaka
created: 2015-09-13T11:00:00Z
modified: 2015-09-18T06:19:07Z
discontinued: false
dominant: false
group u list: _2048_
m user id: 23567
owner id: 23567
owning group id: 9
status: status/Planning
bookmark: false
mutable description: CheZ under strong, constitutive promoter BBa_J23102
mutable notes: none
mutable provenance: Assembly
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0015
component: BBa_J23102
component: BBa_K629003
sequence annotation: BBa_B0015
sequence annotation: BBa_J23102
sequence annotation: BBa_K629003
sequence: https://synbiohub.org/public/igem/BBa_K1631020_sequence/1

BBa_B0015
type: Component
persistent identity: component2455705
display id: component2455705
version: 1
top level: BBa_K1631020
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_J23102
type: Component
persistent identity: component2455691
display id: component2455691
version: 1
top level: BBa_K1631020
access: public
definition: https://synbiohub.org/public/igem/BBa_J23102/1

BBa_K629003
type: Component
persistent identity: component2455698
display id: component2455698
version: 1
top level: BBa_K1631020
access: public
definition: cheZ

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2455705
display id: annotation2455705
version: 1
top level: BBa_K1631020
location: https://synbiohub.org/public/igem/BBa_K1631020/annotation2455705/range2455705/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1631020/annotation2455705/range2455705/1
type: Range
persistent identity: range2455705
display id: range2455705
version: 1
direction: direction/reverse
top level: BBa_K1631020
start: 695
end: 823
orientation: inline

BBa_J23102
type: SequenceAnnotation
persistent identity: annotation2455691
display id: annotation2455691
version: 1
top level: BBa_K1631020
location: https://synbiohub.org/public/igem/BBa_K1631020/annotation2455691/range2455691/1
component: BBa_J23102

https://synbiohub.org/public/igem/BBa_K1631020/annotation2455691/range2455691/1
type: Range
persistent identity: range2455691
display id: range2455691
version: 1
direction: direction/reverse
top level: BBa_K1631020
start: 1
end: 35
orientation: inline

BBa_K629003
type: SequenceAnnotation
persistent identity: annotation2455698
display id: annotation2455698
version: 1
top level: BBa_K1631020
location: https://synbiohub.org/public/igem/BBa_K1631020/annotation2455698/range2455698/1
component: BBa_K629003

https://synbiohub.org/public/igem/BBa_K1631020/annotation2455698/range2455698/1
type: Range
persistent identity: range2455698
display id: range2455698
version: 1
direction: direction/reverse
top level: BBa_K1631020
start: 42
end: 686
orientation: inline

BBa_I712017
type: ComponentDefinition
persistent identity: BBa I712017
display id: BBa_I712017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Chemokine (CXC motif) receptor 4, fused to N-terminal half of ubiquitin.
creator: Anja Korenčič
created: 2007-10-20T11:00:00Z
modified: 2015-08-31T04:07:46Z
discontinued: false
dominant: false
group u list: _130_
m user id: 0
owner id: 1846
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Chemokine (CXC motif) receptor 4, fused to N-terminal half of ubiquitin.
mutable notes: CXCR4 and NUb fragments were fused with PCR overlap extension method without any linker added. The part is cloned into BBa_J52017 (a vector with eukaryontic terminator).
mutable provenance: CXCR4 gene obtained from Origene, NUb gene obtained from Dualsystems.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: NUb
sequence annotation: CXCR4
sequence: https://synbiohub.org/public/igem/BBa_I712017_sequence/1

NUb
type: SequenceAnnotation
persistent identity: annotation1951575
display id: annotation1951575
version: 1
top level: BBa_I712017
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_I712017/annotation1951575/range1951575/1

https://synbiohub.org/public/igem/BBa_I712017/annotation1951575/range1951575/1
type: Range
persistent identity: range1951575
display id: range1951575
version: 1
direction: direction/forward
top level: BBa_I712017
start: 1057
end: 1191
orientation: inline

CXCR4
type: SequenceAnnotation
persistent identity: annotation1951574
display id: annotation1951574
version: 1
top level: BBa_I712017
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_I712017/annotation1951574/range1951574/1

https://synbiohub.org/public/igem/BBa_I712017/annotation1951574/range1951574/1
type: Range
persistent identity: range1951574
display id: range1951574
version: 1
direction: direction/forward
top level: BBa_I712017
start: 1
end: 1056
orientation: inline

BBa_K777103
type: ComponentDefinition
persistent identity: BBa K777103
display id: BBa_K777103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: flhDC operon under the control of constitutive promoter J23105
creator: Team Goettingen
created: 2012-09-18T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The ''flhDC'' operon is the master regulator of motility and chemotaxis in ''E. coli''. FlhDC forms heterotetramers and activates class II operons in concert with sigma factor 70. Among the gene products of class II operons are several components of the flagellum and the alternative sigma factor FliA which is essential for the transcription of class III genes. Here we used a set of 8 constitutive Anderson-promoters from the 2006 Berkeley group to test how different levels of constitutive ''flhDC'' expression affect the motility of ''E. coli''.
mutable notes: * The PstI restriction site in the ''flhDC'' sequence was mutated to CTGCGG using the reverse primer * ''flhDC'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: ** Fwd: 5'-actgaattcgcggccgcttctagatgcatacctccgagttgctg-3' ** Rev: 5'-tcctgcagcggccgctactagttactgcccgggatggcggttgacataagcCgcaggcaaagctgccaacag-3' (the capitalized "C" induces the mutation for removal of the PstI site)
mutable provenance: * The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). * J23105 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K777100
component: BBa_J23105
sequence annotation: BBa_K777100
sequence annotation: BBa_J23105
sequence: https://synbiohub.org/public/igem/BBa_K777103_sequence/1

BBa_K777100
type: Component
persistent identity: component2187038
display id: component2187038
version: 1
top level: BBa_K777103
access: public
definition: BBa_K777100

BBa_J23105
type: Component
persistent identity: component2187030
display id: component2187030
version: 1
top level: BBa_K777103
access: public
definition: https://synbiohub.org/public/igem/BBa_J23105/1

BBa_K777100
type: SequenceAnnotation
persistent identity: annotation2187038
display id: annotation2187038
version: 1
top level: BBa_K777103
location: https://synbiohub.org/public/igem/BBa_K777103/annotation2187038/range2187038/1
component: BBa_K777100

https://synbiohub.org/public/igem/BBa_K777103/annotation2187038/range2187038/1
type: Range
persistent identity: range2187038
display id: range2187038
version: 1
direction: direction/reverse
top level: BBa_K777103
start: 42
end: 910
orientation: inline

BBa_J23105
type: SequenceAnnotation
persistent identity: annotation2187030
display id: annotation2187030
version: 1
top level: BBa_K777103
location: https://synbiohub.org/public/igem/BBa_K777103/annotation2187030/range2187030/1
component: BBa_J23105

https://synbiohub.org/public/igem/BBa_K777103/annotation2187030/range2187030/1
type: Range
persistent identity: range2187030
display id: range2187030
version: 1
direction: direction/reverse
top level: BBa_K777103
start: 1
end: 35
orientation: inline

BBa_K108031
type: ComponentDefinition
persistent identity: BBa K108031
display id: BBa_K108031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TARS-CheZ
creator: Shan Lin
created: 2008-10-22T11:00:00Z
modified: 2015-05-08T01:09:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _222_
m user id: 0
owner id: 2671
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: ...
mutable notes: ...
mutable provenance: ...
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: LVA
sequence annotation: LacI
sequence annotation: TARS
sequence annotation: T7 Terminator
sequence annotation: CheZ
sequence: https://synbiohub.org/public/igem/BBa_K108031_sequence/1

LVA
type: SequenceAnnotation
persistent identity: annotation1986486
display id: annotation1986486
version: 1
top level: BBa_K108031
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K108031/annotation1986486/range1986486/1

https://synbiohub.org/public/igem/BBa_K108031/annotation1986486/range1986486/1
type: Range
persistent identity: range1986486
display id: range1986486
version: 1
direction: direction/forward
top level: BBa_K108031
start: 793
end: 828
orientation: inline

LacI
type: SequenceAnnotation
persistent identity: annotation1986446
display id: annotation1986446
version: 1
top level: BBa_K108031
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K108031/annotation1986446/range1986446/1

https://synbiohub.org/public/igem/BBa_K108031/annotation1986446/range1986446/1
type: Range
persistent identity: range1986446
display id: range1986446
version: 1
direction: direction/forward
top level: BBa_K108031
start: 4
end: 28
orientation: inline

TARS
type: SequenceAnnotation
persistent identity: annotation1986468
display id: annotation1986468
version: 1
top level: BBa_K108031
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K108031/annotation1986468/range1986468/1

https://synbiohub.org/public/igem/BBa_K108031/annotation1986468/range1986468/1
type: Range
persistent identity: range1986468
display id: range1986468
version: 1
direction: direction/forward
top level: BBa_K108031
start: 29
end: 153
orientation: inline

T7 Terminator
type: SequenceAnnotation
persistent identity: annotation1986495
display id: annotation1986495
version: 1
top level: BBa_K108031
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K108031/annotation1986495/range1986495/1

https://synbiohub.org/public/igem/BBa_K108031/annotation1986495/range1986495/1
type: Range
persistent identity: range1986495
display id: range1986495
version: 1
direction: direction/forward
top level: BBa_K108031
start: 829
end: 890
orientation: inline

CheZ
type: SequenceAnnotation
persistent identity: annotation1986469
display id: annotation1986469
version: 1
top level: BBa_K108031
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K108031/annotation1986469/range1986469/1

https://synbiohub.org/public/igem/BBa_K108031/annotation1986469/range1986469/1
type: Range
persistent identity: range1986469
display id: range1986469
version: 1
direction: direction/forward
top level: BBa_K108031
start: 154
end: 825
orientation: inline

BBa_K777105
type: ComponentDefinition
persistent identity: BBa K777105
display id: BBa_K777105
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: flhDC operon under the control of constitutive promoter J23109
creator: Team Goettingen
created: 2012-09-18T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The ''flhDC'' operon is the master regulator of motility and chemotaxis in ''E. coli''. FlhDC forms heterotetramers and activates class II operons in concert with sigma factor 70. Among the gene products of class II operons are several components of the flagellum and the alternative sigma factor FliA which is essential for the transcription of class III genes. Here we used a set of 8 constitutive Anderson-promoters from the 2006 Berkeley group to test how different levels of constitutive ''flhDC'' expression affect the motility of ''E. coli''.
mutable notes: *The PstI restriction site in the ''flhDC'' sequence was mutated to CTGCGG using the reverse primer. *''flhDC'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: **Fwd: 5'-actgaattcgcggccgcttctagatgcatacctccgagttgctg-3' **Rev: 5'-tcctgcagcggccgctactagttactgcccgggatggcggttgacataagcCgcaggcaaagctgccaacag-3' (the capitalized "C" induces the mutation for removal of the PstI site)
mutable provenance: *The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). *J23109 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23109
component: BBa_K777100
sequence annotation: BBa_J23109
sequence annotation: BBa_K777100
sequence: https://synbiohub.org/public/igem/BBa_K777105_sequence/1

BBa_J23109
type: Component
persistent identity: component2187048
display id: component2187048
version: 1
top level: BBa_K777105
access: public
definition: https://synbiohub.org/public/igem/BBa_J23109/1

BBa_K777100
type: Component
persistent identity: component2187056
display id: component2187056
version: 1
top level: BBa_K777105
access: public
definition: BBa_K777100

BBa_J23109
type: SequenceAnnotation
persistent identity: annotation2187048
display id: annotation2187048
version: 1
top level: BBa_K777105
location: https://synbiohub.org/public/igem/BBa_K777105/annotation2187048/range2187048/1
component: BBa_J23109

https://synbiohub.org/public/igem/BBa_K777105/annotation2187048/range2187048/1
type: Range
persistent identity: range2187048
display id: range2187048
version: 1
direction: direction/reverse
top level: BBa_K777105
start: 1
end: 35
orientation: inline

BBa_K777100
type: SequenceAnnotation
persistent identity: annotation2187056
display id: annotation2187056
version: 1
top level: BBa_K777105
location: https://synbiohub.org/public/igem/BBa_K777105/annotation2187056/range2187056/1
component: BBa_K777100

https://synbiohub.org/public/igem/BBa_K777105/annotation2187056/range2187056/1
type: Range
persistent identity: range2187056
display id: range2187056
version: 1
direction: direction/reverse
top level: BBa_K777105
start: 42
end: 910
orientation: inline

BBa_K283030
type: ComponentDefinition
persistent identity: BBa K283030
display id: BBa_K283030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rbs-cheZ_histag-terminators
creator: Nelson Chu, W Ling , Shi Lei
created: 2009-10-13T11:00:00Z
modified: 2015-05-08T01:11:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _383_
m user id: 0
owner id: 5494
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: inducible hypoxia promoter
mutable notes: BBa_283030
mutable provenance: BBa_283030
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0010
component: BBa_B0034
component: BBa_K283008
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_K283008
sequence: https://synbiohub.org/public/igem/BBa_K283030_sequence/1

BBa_B0010
type: Component
persistent identity: component2056941
display id: component2056941
version: 1
top level: BBa_K283030
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component2056936
display id: component2056936
version: 1
top level: BBa_K283030
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K283008
type: Component
persistent identity: component2056940
display id: component2056940
version: 1
top level: BBa_K283030
access: public
definition: BBa_K283008

BBa_B0012
type: Component
persistent identity: component2056943
display id: component2056943
version: 1
top level: BBa_K283030
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2056943
display id: annotation2056943
version: 1
top level: BBa_K283030
location: https://synbiohub.org/public/igem/BBa_K283030/annotation2056943/range2056943/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K283030/annotation2056943/range2056943/1
type: Range
persistent identity: range2056943
display id: range2056943
version: 1
direction: direction/forward
top level: BBa_K283030
start: 828
end: 868
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2056941
display id: annotation2056941
version: 1
top level: BBa_K283030
location: https://synbiohub.org/public/igem/BBa_K283030/annotation2056941/range2056941/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K283030/annotation2056941/range2056941/1
type: Range
persistent identity: range2056941
display id: range2056941
version: 1
direction: direction/forward
top level: BBa_K283030
start: 740
end: 819
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2056936
display id: annotation2056936
version: 1
top level: BBa_K283030
location: https://synbiohub.org/public/igem/BBa_K283030/annotation2056936/range2056936/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K283030/annotation2056936/range2056936/1
type: Range
persistent identity: range2056936
display id: range2056936
version: 1
direction: direction/reverse
top level: BBa_K283030
start: 1
end: 12
orientation: inline

BBa_K283008
type: SequenceAnnotation
persistent identity: annotation2056940
display id: annotation2056940
version: 1
top level: BBa_K283030
location: https://synbiohub.org/public/igem/BBa_K283030/annotation2056940/range2056940/1
component: BBa_K283008

https://synbiohub.org/public/igem/BBa_K283030/annotation2056940/range2056940/1
type: Range
persistent identity: range2056940
display id: range2056940
version: 1
direction: direction/reverse
top level: BBa_K283030
start: 19
end: 731
orientation: inline

BBa_K283003
type: ComponentDefinition
persistent identity: BBa K283003
display id: BBa_K283003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTet-rbs-chez_histag-double terminator
creator: Wei Ling , Nelson Chu, Shi Lei
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _383_
m user id: 0
owner id: 5494
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part consists of a fusion protein invisine-RFP with a double terminator. There is no preceding rbs on the protein coding region.
mutable notes: BBa_K283003
mutable provenance: The invisine coding region is self-designed and the terminators are from the registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K283008
component: BBa_B0034
component: BBa_B0010
component: BBa_B0012
component: BBa_R0040
sequence annotation: BBa_R0040
sequence annotation: BBa_B0012
sequence annotation: BBa_K283008
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_K283003_sequence/1

BBa_K283008
type: Component
persistent identity: component2047852
display id: component2047852
version: 1
top level: BBa_K283003
access: public
definition: BBa_K283008

BBa_B0034
type: Component
persistent identity: component2047848
display id: component2047848
version: 1
top level: BBa_K283003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component2047853
display id: component2047853
version: 1
top level: BBa_K283003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component2047855
display id: component2047855
version: 1
top level: BBa_K283003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0040
type: Component
persistent identity: component2047842
display id: component2047842
version: 1
top level: BBa_K283003
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2047842
display id: annotation2047842
version: 1
top level: BBa_K283003
location: https://synbiohub.org/public/igem/BBa_K283003/annotation2047842/range2047842/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K283003/annotation2047842/range2047842/1
type: Range
persistent identity: range2047842
display id: range2047842
version: 1
direction: direction/forward
top level: BBa_K283003
start: 1
end: 54
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2047855
display id: annotation2047855
version: 1
top level: BBa_K283003
location: https://synbiohub.org/public/igem/BBa_K283003/annotation2047855/range2047855/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K283003/annotation2047855/range2047855/1
type: Range
persistent identity: range2047855
display id: range2047855
version: 1
direction: direction/forward
top level: BBa_K283003
start: 890
end: 930
orientation: inline

BBa_K283008
type: SequenceAnnotation
persistent identity: annotation2047852
display id: annotation2047852
version: 1
top level: BBa_K283003
location: https://synbiohub.org/public/igem/BBa_K283003/annotation2047852/range2047852/1
component: BBa_K283008

https://synbiohub.org/public/igem/BBa_K283003/annotation2047852/range2047852/1
type: Range
persistent identity: range2047852
display id: range2047852
version: 1
direction: direction/reverse
top level: BBa_K283003
start: 81
end: 793
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2047848
display id: annotation2047848
version: 1
top level: BBa_K283003
location: https://synbiohub.org/public/igem/BBa_K283003/annotation2047848/range2047848/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K283003/annotation2047848/range2047848/1
type: Range
persistent identity: range2047848
display id: range2047848
version: 1
direction: direction/reverse
top level: BBa_K283003
start: 63
end: 74
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2047853
display id: annotation2047853
version: 1
top level: BBa_K283003
location: https://synbiohub.org/public/igem/BBa_K283003/annotation2047853/range2047853/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K283003/annotation2047853/range2047853/1
type: Range
persistent identity: range2047853
display id: range2047853
version: 1
direction: direction/forward
top level: BBa_K283003
start: 802
end: 881
orientation: inline

BBa_K094101
type: ComponentDefinition
persistent identity: BBa K094101
display id: BBa_K094101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: plambda P(O-R12)-rbs-cheZ
creator: Shi Lei
created: 2008-10-12T11:00:00Z
modified: 2015-05-08T01:08:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _255_
m user id: 0
owner id: 2589
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part contains a lambda promoter that is constitutively on and can be repressed by CI protein. cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement. This part lacks a terminator.
mutable notes: the terminator is not included in this part
mutable provenance: From non-biobrick plasmid, flanked by biobrick prefix and surfix.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K094100
component: BBa_K094002
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K094100
sequence annotation: BBa_K094002
sequence: https://synbiohub.org/public/igem/BBa_K094101_sequence/1

BBa_K094100
type: Component
persistent identity: component1990804
display id: component1990804
version: 1
top level: BBa_K094101
access: public
definition: BBa_K094100

BBa_K094002
type: Component
persistent identity: component1990800
display id: component1990800
version: 1
top level: BBa_K094101
access: public
definition: https://synbiohub.org/public/igem/BBa_K094002/1

BBa_B0034
type: Component
persistent identity: component1990802
display id: component1990802
version: 1
top level: BBa_K094101
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1990802
display id: annotation1990802
version: 1
top level: BBa_K094101
location: https://synbiohub.org/public/igem/BBa_K094101/annotation1990802/range1990802/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K094101/annotation1990802/range1990802/1
type: Range
persistent identity: range1990802
display id: range1990802
version: 1
direction: direction/reverse
top level: BBa_K094101
start: 109
end: 120
orientation: inline

BBa_K094100
type: SequenceAnnotation
persistent identity: annotation1990804
display id: annotation1990804
version: 1
top level: BBa_K094101
location: https://synbiohub.org/public/igem/BBa_K094101/annotation1990804/range1990804/1
component: BBa_K094100

https://synbiohub.org/public/igem/BBa_K094101/annotation1990804/range1990804/1
type: Range
persistent identity: range1990804
display id: range1990804
version: 1
direction: direction/reverse
top level: BBa_K094101
start: 127
end: 821
orientation: inline

BBa_K094002
type: SequenceAnnotation
persistent identity: annotation1990800
display id: annotation1990800
version: 1
top level: BBa_K094101
location: https://synbiohub.org/public/igem/BBa_K094101/annotation1990800/range1990800/1
component: BBa_K094002

https://synbiohub.org/public/igem/BBa_K094101/annotation1990800/range1990800/1
type: Range
persistent identity: range1990800
display id: range1990800
version: 1
direction: direction/reverse
top level: BBa_K094101
start: 1
end: 100
orientation: inline

BBa_K094106
type: ComponentDefinition
persistent identity: BBa K094106
display id: BBa_K094106
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plux-rbs-CI-terminator-plambda P(O-R12)-rbs-cheZ
creator: Liu Chenli
created: 2008-10-13T11:00:00Z
modified: 2015-05-08T01:08:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _255_
m user id: 0
owner id: 2589
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a part in which the expression of cheZ is repressed by increasing AHL concentration in the environment in the presence of luxR protein. In other circumstances the cheZ gene is constitutively expressed. The cheZ gene lacks a terminator.
mutable notes: N/A
mutable provenance: composited from K094101 and K094160
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K094011
component: BBa_R0062
component: BBa_B0034
component: BBa_K094013
component: BBa_K094100
component: BBa_K094002
sequence annotation: BBa_B0034
sequence annotation: BBa_R0062
sequence annotation: BBa_K094013
sequence annotation: BBa_K094100
sequence annotation: BBa_K094011
sequence annotation: BBa_K094002
sequence: https://synbiohub.org/public/igem/BBa_K094106_sequence/1

BBa_K094011
type: Component
persistent identity: component1991526
display id: component1991526
version: 1
top level: BBa_K094106
access: public
definition: https://synbiohub.org/public/igem/BBa_K094011/1

BBa_R0062
type: Component
persistent identity: component1991520
display id: component1991520
version: 1
top level: BBa_K094106
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_B0034
type: Component
persistent identity: component1991531
display id: component1991531
version: 1
top level: BBa_K094106
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component1991525
display id: component1991525
version: 1
top level: BBa_K094106
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K094013
type: Component
persistent identity: component1991527
display id: component1991527
version: 1
top level: BBa_K094106
access: public
definition: https://synbiohub.org/public/igem/BBa_K094013/1

BBa_K094100
type: Component
persistent identity: component1991533
display id: component1991533
version: 1
top level: BBa_K094106
access: public
definition: BBa_K094100

BBa_K094002
type: Component
persistent identity: component1991529
display id: component1991529
version: 1
top level: BBa_K094106
access: public
definition: https://synbiohub.org/public/igem/BBa_K094002/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1991525
display id: annotation1991525
version: 1
top level: BBa_K094106
location: https://synbiohub.org/public/igem/BBa_K094106/annotation1991525/range1991525/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K094106/annotation1991525/range1991525/1
type: Range
persistent identity: range1991525
display id: range1991525
version: 1
direction: direction/reverse
top level: BBa_K094106
start: 64
end: 75
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation1991520
display id: annotation1991520
version: 1
top level: BBa_K094106
location: https://synbiohub.org/public/igem/BBa_K094106/annotation1991520/range1991520/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K094106/annotation1991520/range1991520/1
type: Range
persistent identity: range1991520
display id: range1991520
version: 1
direction: direction/forward
top level: BBa_K094106
start: 1
end: 55
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1991531
display id: annotation1991531
version: 1
top level: BBa_K094106
location: https://synbiohub.org/public/igem/BBa_K094106/annotation1991531/range1991531/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K094106/annotation1991531/range1991531/1
type: Range
persistent identity: range1991531
display id: range1991531
version: 1
direction: direction/reverse
top level: BBa_K094106
start: 1809
end: 1820
orientation: inline

BBa_K094013
type: SequenceAnnotation
persistent identity: annotation1991527
display id: annotation1991527
version: 1
top level: BBa_K094106
location: https://synbiohub.org/public/igem/BBa_K094106/annotation1991527/range1991527/1
component: BBa_K094013

https://synbiohub.org/public/igem/BBa_K094106/annotation1991527/range1991527/1
type: Range
persistent identity: range1991527
display id: range1991527
version: 1
direction: direction/reverse
top level: BBa_K094106
start: 1599
end: 1692
orientation: inline

BBa_K094100
type: SequenceAnnotation
persistent identity: annotation1991533
display id: annotation1991533
version: 1
top level: BBa_K094106
location: https://synbiohub.org/public/igem/BBa_K094106/annotation1991533/range1991533/1
component: BBa_K094100

https://synbiohub.org/public/igem/BBa_K094106/annotation1991533/range1991533/1
type: Range
persistent identity: range1991533
display id: range1991533
version: 1
direction: direction/reverse
top level: BBa_K094106
start: 1827
end: 2521
orientation: inline

BBa_K094011
type: SequenceAnnotation
persistent identity: annotation1991526
display id: annotation1991526
version: 1
top level: BBa_K094106
location: https://synbiohub.org/public/igem/BBa_K094106/annotation1991526/range1991526/1
component: BBa_K094011

https://synbiohub.org/public/igem/BBa_K094106/annotation1991526/range1991526/1
type: Range
persistent identity: range1991526
display id: range1991526
version: 1
direction: direction/reverse
top level: BBa_K094106
start: 82
end: 1590
orientation: inline

BBa_K094002
type: SequenceAnnotation
persistent identity: annotation1991529
display id: annotation1991529
version: 1
top level: BBa_K094106
location: https://synbiohub.org/public/igem/BBa_K094106/annotation1991529/range1991529/1
component: BBa_K094002

https://synbiohub.org/public/igem/BBa_K094106/annotation1991529/range1991529/1
type: Range
persistent identity: range1991529
display id: range1991529
version: 1
direction: direction/reverse
top level: BBa_K094106
start: 1701
end: 1800
orientation: inline

BBa_K777120
type: ComponentDefinition
persistent identity: BBa K777120
display id: BBa_K777120
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: motB under the control of constitutive promoter J23114
creator: Team Goettingen
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This gene codes for MotB, a part of the flagellar motor. MotA and MotB constitute the stator and are required for the flow of ions and torque generation. Here we used 3 different constitutive [http://partsregistry.org/Part:BBa_J23100 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''motB'' expression affect the motility of ''E. coli''.
mutable notes: * ''motB'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: ** Fwd: 5'-gtttcttcgaattcgcggccgcttctagatgaagaatcaagcgcatcc-3' ** Rev: 5'-gtttcttcctgcagcggccgctactagtatcacctcggttcggctgatgg-3'
mutable provenance: * The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). * J23100 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K777117
component: BBa_J23114
sequence annotation: BBa_K777117
sequence annotation: BBa_J23114
sequence: https://synbiohub.org/public/igem/BBa_K777120_sequence/1

BBa_K777117
type: Component
persistent identity: component2190801
display id: component2190801
version: 1
top level: BBa_K777120
access: public
definition: BBa_K777117

BBa_J23114
type: Component
persistent identity: component2190799
display id: component2190799
version: 1
top level: BBa_K777120
access: public
definition: https://synbiohub.org/public/igem/BBa_J23114/1

BBa_K777117
type: SequenceAnnotation
persistent identity: annotation2190801
display id: annotation2190801
version: 1
top level: BBa_K777120
location: https://synbiohub.org/public/igem/BBa_K777120/annotation2190801/range2190801/1
component: BBa_K777117

https://synbiohub.org/public/igem/BBa_K777120/annotation2190801/range2190801/1
type: Range
persistent identity: range2190801
display id: range2190801
version: 1
direction: direction/reverse
top level: BBa_K777120
start: 42
end: 968
orientation: inline

BBa_J23114
type: SequenceAnnotation
persistent identity: annotation2190799
display id: annotation2190799
version: 1
top level: BBa_K777120
location: https://synbiohub.org/public/igem/BBa_K777120/annotation2190799/range2190799/1
component: BBa_J23114

https://synbiohub.org/public/igem/BBa_K777120/annotation2190799/range2190799/1
type: Range
persistent identity: range2190799
display id: range2190799
version: 1
direction: direction/reverse
top level: BBa_K777120
start: 1
end: 35
orientation: inline

cheB
type: ComponentDefinition
persistent identity: BBa C0024
display id: BBa_C0024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CheB chemotaxis coding sequence (protein glutamate methylesterase)
creator: MIT SBC 2004 (Ken)
created: 2004-08-01T11:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: CheB is part of the chemotaxis pathway in E. coli.
mutable notes: Checked (DE, VC, KN). Part ready for synthesis. 8/3/4
mutable provenance: NCBI - E. Coli strain K12 substrain MG1655
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Help:Barcodes
sequence annotation: CheB
sequence: https://synbiohub.org/public/igem/BBa_C0024_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214017
display id: annotation2214017
version: 1
top level: cheB
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0024/annotation2214017/range2214017/1

https://synbiohub.org/public/igem/BBa_C0024/annotation2214017/range2214017/1
type: Range
persistent identity: range2214017
display id: range2214017
version: 1
direction: direction/forward
top level: cheB
start: 1054
end: 1078
orientation: inline

CheB
type: SequenceAnnotation
persistent identity: annotation1891614
display id: annotation1891614
version: 1
top level: cheB
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0024/annotation1891614/range1891614/1

https://synbiohub.org/public/igem/BBa_C0024/annotation1891614/range1891614/1
type: Range
persistent identity: range1891614
display id: range1891614
version: 1
direction: direction/forward
top level: cheB
start: 1
end: 1053
orientation: inline

BBa_K108004
type: ComponentDefinition
persistent identity: BBa K108004
display id: BBa_K108004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FlgC
creator: Qi Liu
created: 2008-10-19T11:00:00Z
modified: 2015-05-08T01:09:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _222_
m user id: 0
owner id: 2671
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: flagellar component of cell-proximal portion of basal-body rod
mutable notes: PCR using primers with biobrick prefix and surfix, and then cloned into pSB1AC3.
mutable provenance: E.coli K12 DH10B genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: FlgC
sequence: https://synbiohub.org/public/igem/BBa_K108004_sequence/1

FlgC
type: SequenceAnnotation
persistent identity: annotation1986949
display id: annotation1986949
version: 1
top level: BBa_K108004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K108004/annotation1986949/range1986949/1

https://synbiohub.org/public/igem/BBa_K108004/annotation1986949/range1986949/1
type: Range
persistent identity: range1986949
display id: range1986949
version: 1
direction: direction/forward
top level: BBa_K108004
start: 1
end: 402
orientation: inline

BBa_K777101
type: ComponentDefinition
persistent identity: BBa K777101
display id: BBa_K777101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: flhDC operon under the control of constitutive promoter J23100
creator: Team Goettingen
created: 2012-09-18T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The flhDC operon is the master regulator of motility and chemotaxis in E. coli. FlhDC forms heterotetramers and activates class II operons in concert with sigma factor 70. Among the gene products of class II operons are several components of the flagellum and the alternative sigma factor FliA which is essential for the transcription of class III genes. Here we used a set of 8 constitutive promoters from the 2006 Berkeley group to test how different levels of constitutive ''flhDC'' expression affect motility in ''E. coli''.
mutable notes: *The PstI restriction site in the ''flhDC'' sequence was mutated to CTGCGG using the reverse primer. *''flhDC'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: **Fwd: 5'-actgaattcgcggccgcttctagatgcatacctccgagttgctg-3' **Rev: 5'-tcctgcagcggccgctactagttactgcccgggatggcggttgacataagcCgcaggcaaagctgccaacag-3' (the capitalized "C" induces the mutation for removal of the PstI site)
mutable provenance: *The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). *J23100 information was from partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K777100
component: BBa_J23100
sequence annotation: BBa_K777100
sequence annotation: BBa_J23100
sequence: https://synbiohub.org/public/igem/BBa_K777101_sequence/1

BBa_K777100
type: Component
persistent identity: component2186537
display id: component2186537
version: 1
top level: BBa_K777101
access: public
definition: BBa_K777100

BBa_J23100
type: Component
persistent identity: component2186529
display id: component2186529
version: 1
top level: BBa_K777101
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_K777100
type: SequenceAnnotation
persistent identity: annotation2186537
display id: annotation2186537
version: 1
top level: BBa_K777101
location: https://synbiohub.org/public/igem/BBa_K777101/annotation2186537/range2186537/1
component: BBa_K777100

https://synbiohub.org/public/igem/BBa_K777101/annotation2186537/range2186537/1
type: Range
persistent identity: range2186537
display id: range2186537
version: 1
direction: direction/reverse
top level: BBa_K777101
start: 42
end: 910
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2186529
display id: annotation2186529
version: 1
top level: BBa_K777101
location: https://synbiohub.org/public/igem/BBa_K777101/annotation2186529/range2186529/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K777101/annotation2186529/range2186529/1
type: Range
persistent identity: range2186529
display id: range2186529
version: 1
direction: direction/reverse
top level: BBa_K777101
start: 1
end: 35
orientation: inline

BBa_K777116
type: ComponentDefinition
persistent identity: BBa K777116
display id: BBa_K777116
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: motA under the control of constitutive promoter J23114
creator: Team Goettingen
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The ''motA'' gene codes for a part of the flagellar motor complex. MotA proteins represent the proton conducting component of this molecular machine. Thus it is necessary for flagellar rotation. Here we used 3 different constitutive [http://partsregistry.org/Part:BBa_J23100 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''motA'' expression affect the motility or flagellum size of ''E. coli''.
mutable notes: *''motA'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: **Fwd: 5'-gtttcttcgaattcgcggccgcttctagatgaccggtggaagccttgg-3' **Rev: 5'-gtttcttcctgcagcggccgctactagtatcatgcttcctcggttgtcg-3'
mutable provenance: * ''motA'' was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). * J23114 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23114
component: BBa_K777113
sequence annotation: BBa_J23114
sequence annotation: BBa_K777113
sequence: https://synbiohub.org/public/igem/BBa_K777116_sequence/1

BBa_J23114
type: Component
persistent identity: component2189732
display id: component2189732
version: 1
top level: BBa_K777116
access: public
definition: https://synbiohub.org/public/igem/BBa_J23114/1

BBa_K777113
type: Component
persistent identity: component2189734
display id: component2189734
version: 1
top level: BBa_K777116
access: public
definition: BBa_K777113

BBa_J23114
type: SequenceAnnotation
persistent identity: annotation2189732
display id: annotation2189732
version: 1
top level: BBa_K777116
location: https://synbiohub.org/public/igem/BBa_K777116/annotation2189732/range2189732/1
component: BBa_J23114

https://synbiohub.org/public/igem/BBa_K777116/annotation2189732/range2189732/1
type: Range
persistent identity: range2189732
display id: range2189732
version: 1
direction: direction/reverse
top level: BBa_K777116
start: 1
end: 35
orientation: inline

BBa_K777113
type: SequenceAnnotation
persistent identity: annotation2189734
display id: annotation2189734
version: 1
top level: BBa_K777116
location: https://synbiohub.org/public/igem/BBa_K777116/annotation2189734/range2189734/1
component: BBa_K777113

https://synbiohub.org/public/igem/BBa_K777116/annotation2189734/range2189734/1
type: Range
persistent identity: range2189734
display id: range2189734
version: 1
direction: direction/reverse
top level: BBa_K777116
start: 42
end: 872
orientation: inline

BBa_K777118
type: ComponentDefinition
persistent identity: BBa K777118
display id: BBa_K777118
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: motB under the control of constitutive promoter J23100
creator: Team Goettingen
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This gene codes for MotB, a part of the flagellar motor. MotA and MotB constitute the stator and are required for the flow of ions and torque generation.
mutable notes: * ''motB'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: ** Fwd: 5'-gtttcttcgaattcgcggccgcttctagatgaagaatcaagcgcatcc-3' ** Rev: 5'-gtttcttcctgcagcggccgctactagtatcacctcggttcggctgatgg-3'
mutable provenance: * The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). * J23100 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23100
component: BBa_K777117
sequence annotation: BBa_K777117
sequence annotation: BBa_J23100
sequence: https://synbiohub.org/public/igem/BBa_K777118_sequence/1

BBa_J23100
type: Component
persistent identity: component2190662
display id: component2190662
version: 1
top level: BBa_K777118
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_K777117
type: Component
persistent identity: component2190664
display id: component2190664
version: 1
top level: BBa_K777118
access: public
definition: BBa_K777117

BBa_K777117
type: SequenceAnnotation
persistent identity: annotation2190664
display id: annotation2190664
version: 1
top level: BBa_K777118
location: https://synbiohub.org/public/igem/BBa_K777118/annotation2190664/range2190664/1
component: BBa_K777117

https://synbiohub.org/public/igem/BBa_K777118/annotation2190664/range2190664/1
type: Range
persistent identity: range2190664
display id: range2190664
version: 1
direction: direction/reverse
top level: BBa_K777118
start: 42
end: 968
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2190662
display id: annotation2190662
version: 1
top level: BBa_K777118
location: https://synbiohub.org/public/igem/BBa_K777118/annotation2190662/range2190662/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K777118/annotation2190662/range2190662/1
type: Range
persistent identity: range2190662
display id: range2190662
version: 1
direction: direction/reverse
top level: BBa_K777118
start: 1
end: 35
orientation: inline

BBa_K1631019
type: ComponentDefinition
persistent identity: BBa K1631019
display id: BBa_K1631019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pconst(medium) -rbs- cheZ -d.term
creator: Yuto Yamanaka
created: 2015-09-13T11:00:00Z
modified: 2015-09-18T06:20:26Z
discontinued: false
dominant: false
group u list: _2048_
m user id: 23567
owner id: 23567
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Constitutive CheZ generator (medium).
mutable notes: none
mutable provenance: Assembly
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J23110
component: BBa_B0015
component: BBa_K629003
sequence annotation: BBa_J23110
sequence annotation: BBa_B0015
sequence annotation: BBa_K629003
sequence: https://synbiohub.org/public/igem/BBa_K1631019_sequence/1

BBa_J23110
type: Component
persistent identity: component2455611
display id: component2455611
version: 1
top level: BBa_K1631019
access: public
definition: https://synbiohub.org/public/igem/BBa_J23110/1

BBa_B0015
type: Component
persistent identity: component2455625
display id: component2455625
version: 1
top level: BBa_K1631019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K629003
type: Component
persistent identity: component2455618
display id: component2455618
version: 1
top level: BBa_K1631019
access: public
definition: cheZ

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2455611
display id: annotation2455611
version: 1
top level: BBa_K1631019
location: https://synbiohub.org/public/igem/BBa_K1631019/annotation2455611/range2455611/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K1631019/annotation2455611/range2455611/1
type: Range
persistent identity: range2455611
display id: range2455611
version: 1
direction: direction/reverse
top level: BBa_K1631019
start: 1
end: 35
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2455625
display id: annotation2455625
version: 1
top level: BBa_K1631019
location: https://synbiohub.org/public/igem/BBa_K1631019/annotation2455625/range2455625/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1631019/annotation2455625/range2455625/1
type: Range
persistent identity: range2455625
display id: range2455625
version: 1
direction: direction/reverse
top level: BBa_K1631019
start: 695
end: 823
orientation: inline

BBa_K629003
type: SequenceAnnotation
persistent identity: annotation2455618
display id: annotation2455618
version: 1
top level: BBa_K1631019
location: https://synbiohub.org/public/igem/BBa_K1631019/annotation2455618/range2455618/1
component: BBa_K629003

https://synbiohub.org/public/igem/BBa_K1631019/annotation2455618/range2455618/1
type: Range
persistent identity: range2455618
display id: range2455618
version: 1
direction: direction/reverse
top level: BBa_K1631019
start: 42
end: 686
orientation: inline

BBa_K283034
type: ComponentDefinition
persistent identity: BBa K283034
display id: BBa_K283034
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: motB (Backbone: pSB1AK3)
creator: Shi Lei, W Ling , Nelson Chu
created: 2009-10-15T11:00:00Z
modified: 2015-05-08T01:11:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _383_
m user id: 0
owner id: 5494
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The motB protein coding gene on the plasmid pS1AK3
mutable notes: BBa_K283034
mutable provenance: All from the registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_J09271
sequence: https://synbiohub.org/public/igem/BBa_K283034_sequence/1

BBa_J09271
type: SequenceAnnotation
persistent identity: annotation2047442
display id: annotation2047442
version: 1
top level: BBa_K283034
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K283034/annotation2047442/range2047442/1

https://synbiohub.org/public/igem/BBa_K283034/annotation2047442/range2047442/1
type: Range
persistent identity: range2047442
display id: range2047442
version: 1
direction: direction/reverse
top level: BBa_K283034
start: 1
end: 927
orientation: inline