rbs-GFP(LVA)-terminators (Backbone: pSB1AK3)
The chez is responsible for the dephosphorylation of cheY protein in bacteria flagella movement and is tagged by a 6-histidine tail. The pLac promoter can be repressed by LacI repressor protein
The chez-histag coding region is self-designed The pLac promoter , rbs are from the registry
CheZ is responsible for the dephosphorylation of flagellum-regulating protein CheY. It has been reported that cheZ-/- strain has a higher frequency of direction change and thus a narrower range of mobility. This device usually allows strong cheZ expression, while in the presense of IPTG, the transcription of cheZ is repressed by the overexpression of lambda cI repressor BBa_C0051.
cheZ CDS is cloned from Escherichia coli JM109 strain. Other sequences are from BioBrick parts.
CheZ coding sequence (Chemotaxis protein)
CheZ is one of the 6 Che proteins used in chemeotaxis. CheZ is a phosphatase which dephosphorylates CheY which in turn then allows the cell to run and tumble, wheres if CheZ is absence, CheY is phosphorylated, and the cell tumbles exclusively. (Topp and Gallivan J. Am. Chem. Soc. 129, p6865-6873)
CheZ is responsible for the dephosphorylation of flagellum-regulating protein CheY. It has been reported that cheZ-/- strain has a higher frequency of direction change and thus a narrower range of mobility. This device rescues cheZ-/- cells in the presence of IPTG.
FF_FF ansB promoter-rbs-cheZ_histag-terminators
This part consist of a chez protein linked to a double terminator. The cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement. The chez coding region has a 6-Histidine tail and do not contain any rbs.
The chez-histag coding region is self-designed The double terminator is from the registry
The CI repressor protein is constitutively expressed under the pLux promoter. The CI protein represses the transcription of plambda(R-O12). This part lacks a terminator
The CI, terminator, pLambda promoter and the chez gene are solf designed the pLux promoter, rbs are from the registry
assembly protein for flagellar basal-body periplasmic P ring
PCR using primers with biobrick prefix and surfix, and then cloned into pSB1AC3.
motB under the control of constitutive promoter J23112
This gene codes for MotB, a part of the flagellar motor. MotA and MotB constitute the stator and are required for the flow of ions and torque generation. Here we used 3 different constitutive [http://partsregistry.org/Part:BBa_J23100 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''motB'' expression affect the motility of ''E. coli''.
* ''motB'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: ** Fwd: 5'-gtttcttcgaattcgcggccgcttctagatgaagaatcaagcgcatcc-3' ** Rev: 5'-gtttcttcctgcagcggccgctactagtatcacctcggttcggctgatgg-3'
* The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). * J23112 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
AspA is an aspartate ammonia-lyase, which produces aspartate from fumarate and ammonium ion. Aspartate can be used as a chemoattractant for bacteria including Escherichia coli. In this device, AspA CDS, flanked with RBS and terminator, is under control of lacI-regulated strong promoter. IPTG can be used to induce aspartate production.
motB flagellar protein, without endogenous RBS
motB E. coli flagellar protein linking torque machinery to cell wall; allows for induction of motility
We had to design this part to address leaky expression from R0062. In order to induce motility with HSL, we must be able to demonstrate both repression and induction. S03271 contains the endogenous RBS of motB and is thought to be strong. Leaky expression could be diminished by adding weaker ribosome binding sites upstream of this part.
This part was amplified by PCR from the genome of E. coli strain RP437 (wild-type for motility)
constitutive promoter-rbs-CI (lambda LVA)
This part generates chez protein under a hypoxia inducible promoter panas. The cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement and is tagged by a 6-Histidine tail.
The rbs, double terminator comes from the registry
The motA gene codes for a part of the flagellar motor complex. MotA proteins represent the proton conducting component of this molecular machine. Thus it is necessary for flagellar rotation.
*''motA'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: **Fwd: GTTTCTTCGAATTCGCGGCCGCTTCTAGATGACCGGTGGAAGCCTTGG **Rev: GTTTCTTCCTGCAGCGGCCGCTACTAGTATCATGCTTCCTCGGTTGTCG
* The part was amplified from genomic DNA of E. coli str. K-12 substr. DH10B, complete genome (CP000948.1).
flhDC operon under the control of constitutive promoter J23114
The ''flhDC'' operon is the master regulator of motility and chemotaxis in ''E. coli''. FlhDC forms heterotetramers and activates class II operons in concert with sigma factor 70. Among the gene products of class II operons are several components of the flagellum and the alternative sigma factor FliA which is essential for the transcription of class III genes. Here we used a set of 8 constitutive Anderson-promoters from the 2006 Berkeley group to test how different levels of constitutive ''flhDC'' expression affect the motility of ''E. coli''.
*The PstI restriction site in the ''flhDC'' sequence was mutated to CTGCGG using the reverse primer. *''flhDC'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: **Fwd: 5'-actgaattcgcggccgcttctagatgcatacctccgagttgctg-3' **Rev: 5'-tcctgcagcggccgctactagttactgcccgggatggcggttgacataagcCgcaggcaaagctgccaacag-3' (the capitalized "C" induces the mutation for removal of the PstI site)
*The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). *J23100 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
Biofilm formation device. yddV gene with the IPTG inducible promoter.
Gene yddV is under the regulation of Ptrc promoter (IPTG-inducible). yddV is a Diguanylate Cyclase-Genomic. The product of gene yddV has diguanylate cyclase (DGC) activity. DGC uses 2 GTP to form a Bis-(3???-5???)-cyclic dimeric guanosine monophosphate (c-di-GMP). C-di-GMP is a global second messenger in bacteria. Biofilm formation of E.coli is manipulable by varying c-di-GMP concentrations. When the c-di-GMP level stays low, there is little biofilm and the bacteria is dispersive. High concentrations of c-di-GMP promote bacteria to form more cellulose and fimbriae, which enhances the biofilm formation and decrease the motility of bacteria.
This gene codes for MotB, a part of the flagellar motor. MotA and MotB constitute the stator and are required for the flow of ions and torque generation.
* motA was amplified from genomic DNA of E. coli DH10B via PCR using the following primers: ** Fwd: GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAAGAATCAAGCGCATCC ** Rev: GTTTCTTCCTGCAGCGGCCGCTACTAGTATCACCTCGGTTCGGCTGATGG
fliC under the control of constitutive promoter J23100
The ''fliC'' gene codes for flagellin. This protein is the subunit that forms the flagellar filament of ''E. coli''. Here we used 3 different constitutive [http://partsregistry.org/Part:BBa_J23100 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''fliC'' expression affect the motility or flagellum size of ''E. coli''.
* The ''fliC'' gene contained three PstI sites and one SpeI site that had to be removed. These mutations were induced with different primers via overlap-PCR. * ''fliC'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: ** Fwd: 5'-gtttcttcgaattcgcggccgcttctagatggcacaagtcattaatacc-3' ** Rev: 5'-gtttcttcgaattcgcggccgcttctagttaaccctgGagcagagacagaacc-3' (the capitalized "G" induces the mutation for removal of the third PstI site)
* The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1).
fliC under the control of constitutive promoter J23114
* The ''fliC'' gene contained three PstI sites and one SpeI site that had to be removed. These mutations were induced with different primers via overlap-PCR.
* The ''fliC'' gene was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). * J23100 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
Speed control device (pLacI-rbs-TetR-terminator-ptet-rbs-chez_his-double terminators)
This is a device that enable the control of chez(histag) using IPTG. The pLacIq promoter enables high level of expression of the LacI repressor protein. The LacI protein represses the Plac promoter. IPTG binds with LacI repressor protein and remove the inhibiting effect of the LacI on the pLac promoter.
The rbs, terminator and the pLac promoter are from the registry, the rest of the part are self designed
flhDC operon under the control of constitutive promoter J23106
* The PstI restriction site in the ''flhDC'' sequence was mutated to CTGCGG using the reverse primer * ''flhDC'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: ** Fwd: 5'-actgaattcgcggccgcttctagatgcatacctccgagttgctg-3' ** Rev: 5'-tcctgcagcggccgctactagttactgcccgggatggcggttgacataagcCgcaggcaaagctgccaacag-3' (the capitalized "C" induces the mutation for removal of the PstI site)
* The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). * J23106 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
flagellar motor switching and energizing component
* The ''fliC'' gene codes for flagellin. This protein is the subunit that forms the flagellar filament of ''E. Coli''.
* The ''fliC'' gene contained three PstI sites and one SpeI site that had to be removed. These mutations were induced with different primers via overlap-PCR. * ''flhDC'' was amplified from genomic DNA of E. coli DH10B via PCR using the following primers: ** Fwd: ACTGAATTCGCGGCCGCTTCTAGATGCATACCTCCGAGTTGCTG ** Rev: TCCTGCAGCGGCCGCTACTAGTTACTGCCCGGGATGGCGGTTGACATAAGCcGCAGGCAAAGCTGCCAACAG
Naoki Saito,Daiki Haraguchi,Yoshiharu Otaki,Ryuhei Minei
This part consist of a chez downstream of a pLac promoter. The cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement. The pLac promoter can be repressed by LacI protein
The chez coding region is self-designed The rbs and the pLac promomter are from the registry
CheZ is responsible for the dephosphorylation of flagellum-regulating protein CheY. It has been reported that cheZ-/- strain has a higher frequency of direction change and thus a narrower range of mobility. With a promoter of your interest, this device rescues the mobility of cheZ-/- cells.
motA under the control of constitutive promoter J23100
The ''motA'' gene codes for a part of the flagellar motor complex. MotA proteins represent the proton conducting component of this molecular machine. Thus it is necessary for flagellar rotation. Here we used 3 different constitutive [http://partsregistry.org/Part:BBa_J23100 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''motA'' expression affect the motility or flagellum size of ''E. coli''.
*''motA'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: **Fwd: 5'-gtttcttcgaattcgcggccgcttctagatgaccggtggaagccttgg-3' **Rev: 5'-gtttcttcctgcagcggccgctactagtatcatgcttcctcggttgtcg-3'
* ''motA'' was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). * J23100 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
This part contains a strong rbs and a TetR tagged with a LVA degradation tail. The TetR repressor can be used to repress ptet promoter and the TetR coding region do not contain any terminator
yhjH under the control of constitutive promoter J23100
The product of this gene is a phosphodiesterase which is involved in regulating the levels of c-di-GMP, a bacterial signaling molecule. High levels of c-di-GMP promote biofilm synthesis and inhibit flagella by binding to YcgR which then acts as a "brake" on FliG and FliM. Levels of c-di-GMP are elevated and motility is reduced in ''yhjH''-mutants. Here we used 3 different constitutive [http://partsregistry.org/Part:BBa_J23100 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''yhjH'' expression affect the motility of ''E. coli''.
* ''yhjH'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: ** Fwd: 5'-gtttcttcgaattcgcggccgcttctagatgataaggcaggttatccagc-3' ** Rev: 5'-gtttcttcctgcagcggccgctactagtattatagcgccagaaccgccgtattcagc-3'
* ''yhjH'' was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). * J23100 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
Luminesensor repressible SulA408 promoter+CheZ
CheZ coding sequence ligated to the Luminesensor repressible SulA408 promoter.
CheZ is got from E.Coli strain MG1655 by colony PCR
flhDC operon under the control of constitutive promoter J23112
*The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). *J23112 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
fliC under the control of constitutive promoter J23112
* The ''fliC'' gene was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1).
The product of this gene is a phosphodiesterase which is involved in regulating the levels of c-di-GMP, a bacterial signaling molecule. High levels of c-di-GMP promote biofilm synthesis and inhibit flagella by binding to YcgR which then acts as a "brake" on FliG and FliM. Levels of c-di-GMP are elevated and motility is reduced in ''yhjH''-mutants.
* ''yhjH'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: ** Fwd: 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGATAAGGCAGGTTATCCAGC-3' ** Rev: 5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATAGCGCCAGAACCGCCGTATTCAGC-3'
The part was amplified from genomic DNA of E. coli str. K-12 substr. DH10B, complete genome (CP000948.1).
flagellar protein of basal-body outer-membrane L ring
motA under the control of constitutive promoter J23112
flhDC operon under the control of constitutive promoter J23113
*The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). *J23113 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
The pLac promoter can be repressed by LacI protein. The cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement and is tagged by 6-Histidine tail.
The chez-Histag coding region is self-designed The pLac promoter, rbs and the double terminator are from the registry
flagellar component of cell-proximal portion of basal-body rod
Chemokine (C-C motif) receptor 5, a HIV-1 co-receptor.
The part is cloned into BBa_J52017, a vector with eukaryontic terminator. PstI and EcoRI restriction sites inside CCR5 gene were mutated.
The chez is constitutively expressed under the pTet promoter. The pTet promoter can be repressed by TetR repressor protein. The cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement.
The chez coding region is self-designed The pTet promoter, rbs and the terminator are from the registry
cheZ ligated to a medium promoter. This part express CheZ protein mediumly and will induce medium mobility in delta cheZ E.coli strain.
There is a point mutation at position 56 of CheZ coding sequence with have little effect to its function and the bases corresponding to stop codon is changed to bases coding a more frequently used stop codon.
The promoter used here is from Partsregistry. The CheZ gene is from E.coli strain MG1655 by colony PCR and aligned with CheZ sequence at EcoGene database.
yhjH under the control of constitutive promoter J23114
* ''yhjH'' was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). * J23112 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
yhjH under the control of constitutive promoter J23112
proton conductor component of flagella motor
Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
Coding sequence for AspA enzyme. AspA aminates fumarate to make aspartate. Aspartate can be used as a bacterial chemotaxis signal.
The start codon was changed from GTG to ATG to match BioBricks standards. Five silent mutations were introduced to eliminate EcoRI and PstI restriction enzyme sites. EcoRI sites were eliminated at bases 588 and 657 with C -> T mutations. PstI sites were eliminated at bases 624, 999, and 1125 with G -> A mutations. The mutations were designed to match codons with similar frequencies of usage in E. coli.
CheZ is responsible for the dephosphorylation of flagellum-regulating protein CheY. It has been reported that cheZ-/- strain has a higher frequency of direction change and thus a narrower range of mobility.
Cloned from Escherichia coli JM109 strain.
This part contains the cheZ operon coding region without an rbs nor a terminator. cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement.
This part is without promoter, rbs or terminator.
The origin of the cheZ gene comes from E. Coli strain MG1655 chromosome.
The cheZ motility protein is a protein kinase which dephosphorylates cheY. cheY regulates the clockwise and counterclockwise rotation of the motor flagella. If cheY is phosphorylated the motor flagella are not in sync and the bacterium tumbles. If cheY is not phosphorylated the motor flagella are in sync and the bacterium runs (often towards a chemoattractant)
FF_FF ansB promoter-rbs- GFP (LVA)- terminators
This part consist of a strong rbs and the cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement and is tagged by a 6-Histidine tail. This chez coding region do not contain any terminator
The chez-Histag coding region is self-designed The rbs, double terminator are from the registry
Upon recognition of a small molecule (e.g. nutrients or toxins), chemoreceptors transduce a signal through a set of Che proteins. This group of methylesterases and phosphatases regulates the rotation of the flagella. At the end of the chain of Che proteins is CheY. In phosphorylated state, CheY binds the flagella and causes the cells to tumble. The phosphatase CheZ dephosphorylates CheY, inducing dissociation of CheY from the flagella enabling the cells to swim. Cells lacking the CheZ protein are unable to swim and will tumble excessively and incessantly.
1 bp in BBa_K629003 was adapted to create an original restriciton site in the plasmid. CheZ was fused to GFP (BBa_K082003) and its LVA tag was codon-optimized to avoid introducing artificial DNA repetitions.
DNA sequences originate from the E. Coli genome and previous biobrick sequences.
flhDC operon under the control of constitutive promoter J23104
The flhDC operon is the master regulator of motility and chemotaxis in E. coli. FlhDC forms heterotetramers and activates class II operons in concert with sigma factor 70. Among the gene products of class II operons are several components of the flagellum and the alternative sigma factor FliA which is essential for the transcription of class III genes. Here we used a set of 8 constitutive Anderson-promoters from the 2006 Berkeley group to test how different levels of constitutive flhDC expression affect the motility of E. coli.
* The PstI restriction site in the flhDC sequence was mutated to CTGCGG using the reverse primer * flhDC was amplified from genomic DNA of E. coli DH10B via PCR using the following primers: ** Fwd: 5'-actgaattcgcggccgcttctagatgcatacctccgagttgctg-3' ** Rev: 5'-tcctgcagcggccgctactagttactgcccgggatggcggttgacataagcCgcaggcaaagctgccaacag-3' (the capitalized "C" induces the mutation for removal of the PstI site)
* The part was amplified from genomic DNA of E. coli str. K-12 substr. DH10B, complete genome (CP000948.1). * J23100 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
DNA sequences originate from the E. Coli genome and previous biobrick sequences. The ribosome binding site was chosen from the Anderson RBS family.
This part consists of a chez coding region followed by a 6-Histidine tail
The flhDC operon is the master regulator of motility and chemotaxis in E. coli. FlhDC forms heterotetramers and activates class II operons in concert with sigma factor 70. Among the gene products of class II operons are several components of the flagellum and the alternative sigma factor FliA which is essential for the transcription of class III genes.
The PstI restriction site was mutated to CTGCGG using the reverse primer.
This is a cheZ gene that can be induced by luxR protein under high AHL concentration. cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement.
composited from E. Coli cheZ gene and lux promoter
This is the gene coding for cheY protein which controls flagella movement. This sequence, however, is mutated to give a mutant cheY protein which will not respond to the presence of cheZ protein. This part contains the coding region only.
This part contains only the coding region, it is without promoter, rbs and terminator.
This gene is adapted from E. Coli strain MG1655 with two point mutations (D13K Y106W)
cheZ, chemotaxis regulator, protein phosphatase for CheY
Polar localization is dependent on CheA-short. CheZ is a cytosolic phosphatase which functions in the chemotaxis signal transduction complex. Plays an important role in bacterial chemotaxis signal transduction pathway by accelerating the dephosphorylation of phosphorylated CheY (CheY-P).
how to control the proper expression of cheZ
CheZ generator (under constitutive promoter BBa_J23102)
CheZ under strong, constitutive promoter BBa_J23102
Chemokine (CXC motif) receptor 4, fused to N-terminal half of ubiquitin.
CXCR4 and NUb fragments were fused with PCR overlap extension method without any linker added. The part is cloned into BBa_J52017 (a vector with eukaryontic terminator).
CXCR4 gene obtained from Origene, NUb gene obtained from Dualsystems.
flhDC operon under the control of constitutive promoter J23105
* The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). * J23105 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
flhDC operon under the control of constitutive promoter J23109
*The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). *J23109 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
This part consists of a fusion protein invisine-RFP with a double terminator. There is no preceding rbs on the protein coding region.
The invisine coding region is self-designed and the terminators are from the registry
This part contains a lambda promoter that is constitutively on and can be repressed by CI protein. cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement. This part lacks a terminator.
the terminator is not included in this part
From non-biobrick plasmid, flanked by biobrick prefix and surfix.
Plux-rbs-CI-terminator-plambda P(O-R12)-rbs-cheZ
This is a part in which the expression of cheZ is repressed by increasing AHL concentration in the environment in the presence of luxR protein. In other circumstances the cheZ gene is constitutively expressed. The cheZ gene lacks a terminator.
motB under the control of constitutive promoter J23114
* The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). * J23100 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
CheB chemotaxis coding sequence (protein glutamate methylesterase)
CheB is part of the chemotaxis pathway in E. coli.
Checked (DE, VC, KN). Part ready for synthesis. 8/3/4
NCBI - E. Coli strain K12 substrain MG1655
flhDC operon under the control of constitutive promoter J23100
The flhDC operon is the master regulator of motility and chemotaxis in E. coli. FlhDC forms heterotetramers and activates class II operons in concert with sigma factor 70. Among the gene products of class II operons are several components of the flagellum and the alternative sigma factor FliA which is essential for the transcription of class III genes. Here we used a set of 8 constitutive promoters from the 2006 Berkeley group to test how different levels of constitutive ''flhDC'' expression affect motility in ''E. coli''.
*The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). *J23100 information was from partsregistry and physical DNA from the 2012 distribution kit.
motA under the control of constitutive promoter J23114
* ''motA'' was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). * J23114 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
motB under the control of constitutive promoter J23100
The motB protein coding gene on the plasmid pS1AK3