BglII endonuclease with ribosome binding site
BglII nuclease with ribosome binding site and stop codon
This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites. --- PCR mv032/mv010 on pBca9145-Bca1147 (722bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112121 ----------------------------------------------- mv032 Forward Biobricking of rbs.r.BglII! cgataGAATTCatgAGATCTtggaaggagacactcataatgaagattgatataacggactataaccatgc mv010 Reverse Biobricking of r.BglII! cgttaGGATCCttaatatgtcacgattgttcctc
PCR mv032/mv010 on pBca9145-Bca1147 (722bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112121
ori 7 region of Saccharomyces cerevisiae mtDNA, works as replication initial site.
NCBI database of Saccharomyces cerevisiae's genome
Constitutive promoter + 37℃ induced RBS + tetR + double terminator
we took advantage of the interaction between 37?? C induced RBS, tetR gene and pTet promoter to create the temperature regulating circuit. The following is a description of our prescribed strategy. First, we use the constitutive promoter, 37?? C induced RBS (K115002), the tetR gene and terminators. We will call this part the ???Regulator???. We speculate that when the ???Regulator??? and ???Production??? sections are combined, we can achieve a high bacteria growth rate and close to no target protein producing rate at T>37??C, and low bacteria growth rate and high target protein producing rate at T<37??C.
To regulate following gene by temperature
37℃ induced RBS + tetR + double terminator
It is a transcriptional unit including 37℃ induced RBS + tetR + double terminator. It can be added various promoter to adapt your conditions.
To regulate following genes by temperature. To maintain cells at 37?? C ↑ : the tetR protein be generated and repress Ptet's circuit. To release cells at 37?? C ↓ : the tetR protein not be generated and do not repress Ptet's circuit.
the 37℃ induced RBS cames from iGEM08_TUDelft
---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
PCR mv031/mv006 on pBca9145-I716210 (692bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112120 ----------------------------------------------- mv031 Forward Biobricking of rbs.r.BamH1! cgataGAATTCatgAGATCTtaagtgggggcaaattagaatggaagtagaaaaagagtttattactgatgaagc mv006 Reverse Biobricking of r.BamH1! cgttaGGATCCctatttgttttcaactttatctttcc
T7polymerase coding region under a prosseA promoter
The T7polymerase, prosseA promoter is self-desgined The rbs is from the registry
PoPS --> N4 gp8 (DNA replication inhibitor)
Rashmi Sharma, Bryce Bajar, Kendrick Wang
This part is in biobricks 2.0 with BamHI and BglII sites instead of PstI and SpeI sites
This part codes for a Cys2His2 zinc finger protein to be used in assembly of zinc finger polymers (to be continued).
Special RFC needs to be constructed for this purpose
DNA Polymerase Inhibitor (via N4 coliphage gp8)
Coding region of Thiamine E from synechocystis
Constitutive promoter+37℃ induced RBS+tetR+double terminator+Ptet+RBS+GFP+double terminator
We want to utilize a temperature-regulated mechanism to control the expression period of a crystal protein. Therefore, we took advantage of the interaction between 37?? C induced RBS, tetR gene and pTet promoter to create the temperature regulating circuit. The following is a description of our prescribed strategy. First, we use the constitutive promoter, 37?? C induced RBS (K115002), the tetR gene and terminators. We will call this part the ???Regulator???.
The iGEM team Groningen 2016 worked on bioencryption in order to safely store data in DNA. In our project we were mainly working with two sequences of DNA which were the encrypted message sequence and the encryption key sequence. The message sequence was created by our software. It is therefore artificial DNA. We ordered the encrypted message sequence as gBlock from IDT (Integrated DNA technologies).
Created by our CryptoGErM software. http://2016.igem.org/Team:Groningen/Encoding