Kwabena O. Duedu, Chris French, Sahreena Lakhundi
Exo- and endo- glucanases from the cellulolytic bacterium Cellulomonas fimi under the E. coli spac promoter (BBa_J15506). Together with betaglucosidases, the bacterium degrades cellulose into D-glucose.
Cex is a GC rich part and requires extended denaturation with glycerol or commercial GC enhancers
Endoglucanase gene CelC from Agrobacterium tumefaciens C58.
This is Endoglucanase gene (CelC) from the genome of Agrobacterium tumefaciens C58.
The source ofEndoglucanase genes (CelC) is GenBank(NC_003063).
cenA coding sequence encoding Cellulomonas fimi endoglucanase A
The cellulolytic bacterium ''Cellulomonas fimi'' uses 3 endoglucanase (including CenA) and an exoglucanase in the degradation of cellulose into cellobiose, before use B-glucosidase to catalyse the conversion of cellobiose to D-glucose.
Purified ''Cellulomonas fimi'' genomic DNA.
Exo- and endo- glucanases from the cellulolytic bacterium Cellulomonas fimi under the E. coli lac promoter. Together with betaglucosidases, the bacterium degrades cellulose into D-glucose.
Cex is a GC rich part and may require extended denaturation in amplifying the gene.
We used Rybosome Binding Site BBa_B0034 designed by by Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr, evaluated by Warsaw's 2010 team. we added membrane protein signal peptide phoA and cellulase immediately next to it
Since this part is supposed to be attached to part BBa_K633001 in order to geerate part BBa_K633014, we had to begin with both the signal peptide and then the extracelular domain, which is located on the N terminal side of the polypeptide chain.
Endoglucanase A (CenA) from the cellulolytic bacterium Cellulomonas fimi (accession M15823) used in the degradation of cellulose into cellobiose, before using beta-glucosidase to catalyse the conversion of cellobiose to D-glucose.
Cellulase with a mutation, giving it a smalle km
It also has a SacI site and a NheI site after prefix, and before suffix EcorI XbaI SacI Cellulase NheI SpeI PstI
cex coding sequence encoding Cellulomonas fimi exoglucanase
The fungus ''Cellulomonas fimi'' uses an exoglucanase (from ''cex'') along with 3 endoglucanases in the degradation of cellulose into cellobiose, before use B-glucosidase to catalyse the conversion of cellobiose to D-glucose.
The original sequence had a PstI site for 781-786. We added the silent mutation CAG->CAA (Gln) by MABEL to remove this site.
beta-glucosidase gene bglX (chu_2268) from Cytophaga hutchinsonii
This is a beta-glucosidase gene (bglX, locus chu_2268) from the genome sequence of the cellulose-degrading Gram negative bacterium Cytophaga hutchinsonii.
Genome sequence of the cellulose-degrading Gram negative bacterium Cytophaga hutchinsonii.
RBS + Exoglucanase (cex, BBa_K118022) from the cellulolytic bacterium Cellulomonas fimi (accession M15824).
GC rich part. Extended denaturation stage in PCR with glycerol or commercial GC rich enhancers may be required
Cellulomonas fimi exoglucanase cex (accession M15824) with RBS
AraC+Pbad promoter+RBS+signal peptide phoA+Cellulase+Linker+estA membrane protein
The composite part includes a functional expression vector for autotransporter membrane protein estA, with a linker united to a cellulase, attached to a membrane signaling peptide. This was our final construct missing a terminator