//function/coliroid
type: Collection
persistent identity: function coliroid
display id: function_coliroid
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K338029
member: BBa_K1660004
member: BBa_I15008
member: nickname
member: BBa_K1660006
member: BBa_M30109
member: lacZ a
member: BBa_K1587008
member: BBa_K098010
member: BBa_I15009
member: BBa_I15010
member: BBa_B0034
member: BBa_K1660007
member: BBa_K1660005
member: OmpR
member: cph8
member: BBa_K098011

cph8
type: ComponentDefinition
persistent identity: BBa K592000
display id: BBa_K592000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cph8 (Cph1/EnvZ fusion)
creator: Lei Sun
created: 2011-09-17T11:00:00Z
modified: 2015-05-08T01:12:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _763_
m user id: 0
owner id: 7929
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is identical to BBa_I15010, the cph8 red light sensor. We were unable to transform BBa_I15010 from the the 2011 iGEM distribution kit. PCR attempts using VF2 and VR failed to amplify any DNA from the well sample. In the end, we chose to amplify cph8 from Jeffrey Tabor's pJT122 plasmid provided by Chris Voigt. The cph8 in pJT122 contained an internal PstI site, which was removed by point-mutagenesis. The resultant DNA sequence is identical to that of BBa_I15010.
mutable notes: The source DNA (pJT122) contained illegal restriction site (PstI) that had to be removed.
mutable provenance: Jeffrey Tabor's pJT122 plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cph8
sequence: https://synbiohub.org/public/igem/BBa_K592000_sequence/1

cph8
type: SequenceAnnotation
persistent identity: annotation2132074
display id: annotation2132074
version: 1
top level: cph8
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K592000/annotation2132074/range2132074/1

https://synbiohub.org/public/igem/BBa_K592000/annotation2132074/range2132074/1
type: Range
persistent identity: range2132074
display id: range2132074
version: 1
direction: direction/forward
top level: cph8
start: 1
end: 2238
orientation: inline

BBa_K1660007
type: ComponentDefinition
persistent identity: BBa K1660007
display id: BBa_K1660007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PompR-6N-gp35-Terminator
creator: Dai Yuanyi
created: 2015-09-07T11:00:00Z
modified: 2015-09-08T12:46:10Z
discontinued: false
dominant: false
group u list: _2078_
m user id: 26933
owner id: 26933
owning group id: 9
status: status/Planning
bookmark: false
mutable description: PompR is a OmpR-controlled promoter which can be positively regulated by phosphorylated OmpR. This promoter is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription. We insert PompR(BBa_R0082) sequences into the upstream region of our target gene gp35 through restriction-ligation method, which together is connected to vector PSB1C3 afterwards, and therefore we are able to regulate the expression of in through the control of light. Bxb1 gp35 is a serine integrase in Mycobacterium phage Bxb1. This intergrase can exclusively catalyze site-specific recombination. The dismiss of light singal can drive the expression of integrase, which alone can set a DNA sequence(there it means the promoter) flanked by oppositional attB and attP sites, thereby produce an inverted sequence flanked by attL and attR sites. As a consequence, the promotor moves on to express the toxic protein instead of the attractant. We insert the gp35 gene into the downstream region of the PompR promotor by means of 3A synthesis so that the expression of integrase can be regulated by light. In addition, we add a 6N promotor between PompR and gp35 to decrease basal integrase expression above flipping threshold level which could lower the efficiency of int translation. Meanwhile, we insert the recombined sites of attB and attP into the flanking sequences of our constitutive promotor (BBa_J23110 ) and so far we accomplish our reverse promotor system.
mutable notes: we add a 6N promotor between PompR and gp35 to decrease basal integrase expression above flipping threshold level which could lower the efficiency of int translation.
mutable provenance: It comes from the register.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1433008
component: BBa_R0082
sequence annotation: BBa_R0082
sequence annotation: BBa_K1433008
sequence: https://synbiohub.org/public/igem/BBa_K1660007_sequence/1

BBa_K1433008
type: Component
persistent identity: component2446523
display id: component2446523
version: 1
top level: BBa_K1660007
access: public
definition: https://synbiohub.org/public/igem/BBa_K1433008/1

BBa_R0082
type: Component
persistent identity: component2446518
display id: component2446518
version: 1
top level: BBa_K1660007
access: public
definition: OmpR

BBa_R0082
type: SequenceAnnotation
persistent identity: annotation2446518
display id: annotation2446518
version: 1
top level: BBa_K1660007
location: https://synbiohub.org/public/igem/BBa_K1660007/annotation2446518/range2446518/1
component: BBa_R0082

https://synbiohub.org/public/igem/BBa_K1660007/annotation2446518/range2446518/1
type: Range
persistent identity: range2446518
display id: range2446518
version: 1
direction: direction/reverse
top level: BBa_K1660007
start: 1
end: 108
orientation: inline

BBa_K1433008
type: SequenceAnnotation
persistent identity: annotation2446523
display id: annotation2446523
version: 1
top level: BBa_K1660007
location: https://synbiohub.org/public/igem/BBa_K1660007/annotation2446523/range2446523/1
component: BBa_K1433008

https://synbiohub.org/public/igem/BBa_K1660007/annotation2446523/range2446523/1
type: Range
persistent identity: range2446523
display id: range2446523
version: 1
direction: direction/reverse
top level: BBa_K1660007
start: 117
end: 1773
orientation: inline

BBa_K1660006
type: ComponentDefinition
persistent identity: BBa K1660006
display id: BBa_K1660006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23100-B0034-cph8
creator: Dai Yuanyi
created: 2015-09-07T11:00:00Z
modified: 2015-09-08T12:31:43Z
discontinued: false
dominant: false
group u list: _2078_
m user id: 26933
owner id: 26933
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is the light sensor Cph8 with a promoter and RBS.
mutable notes: We constructed it through pcr.
mutable provenance: It comes from the register.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K592000
component: BBa_B0034
component: BBa_J23100
sequence annotation: BBa_J23100
sequence annotation: BBa_K592000
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1660006_sequence/1

BBa_K592000
type: Component
persistent identity: component2446442
display id: component2446442
version: 1
top level: BBa_K1660006
access: public
definition: cph8

BBa_B0034
type: Component
persistent identity: component2446440
display id: component2446440
version: 1
top level: BBa_K1660006
access: public
definition: BBa_B0034

BBa_J23100
type: Component
persistent identity: component2446438
display id: component2446438
version: 1
top level: BBa_K1660006
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2446438
display id: annotation2446438
version: 1
top level: BBa_K1660006
location: https://synbiohub.org/public/igem/BBa_K1660006/annotation2446438/range2446438/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K1660006/annotation2446438/range2446438/1
type: Range
persistent identity: range2446438
display id: range2446438
version: 1
direction: direction/reverse
top level: BBa_K1660006
start: 1
end: 35
orientation: inline

BBa_K592000
type: SequenceAnnotation
persistent identity: annotation2446442
display id: annotation2446442
version: 1
top level: BBa_K1660006
location: https://synbiohub.org/public/igem/BBa_K1660006/annotation2446442/range2446442/1
component: BBa_K592000

https://synbiohub.org/public/igem/BBa_K1660006/annotation2446442/range2446442/1
type: Range
persistent identity: range2446442
display id: range2446442
version: 1
direction: direction/reverse
top level: BBa_K1660006
start: 62
end: 2299
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2446440
display id: annotation2446440
version: 1
top level: BBa_K1660006
location: https://synbiohub.org/public/igem/BBa_K1660006/annotation2446440/range2446440/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1660006/annotation2446440/range2446440/1
type: Range
persistent identity: range2446440
display id: range2446440
version: 1
direction: direction/reverse
top level: BBa_K1660006
start: 44
end: 55
orientation: inline

BBa_K098010
type: ComponentDefinition
persistent identity: BBa K098010
display id: BBa_K098010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HO-pcyA, low expression
creator: Harvard iGEM 08
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:08:41Z
discontinued: false
dominant: false
experience: experience/None
group u list: _196_
m user id: 0
owner id: 3389
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: *Heme oxygenase and phycocyanobilin:ferredoxin oxidoreductase *Used for light sensing system
mutable notes: obtained via PCR
mutable provenance: pPCB-AmpR plasmid from Jeff Tabor (?)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: RBS pprotet
sequence annotation: BBa_B0015 (terminator)
sequence annotation: BBa_B0032
sequence annotation: BBa_J23113
sequence annotation: HO
sequence annotation: pcyA
sequence: https://synbiohub.org/public/igem/BBa_K098010_sequence/1

RBS pprotet
type: SequenceAnnotation
persistent identity: annotation1990294
display id: annotation1990294
version: 1
top level: BBa_K098010
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K098010/annotation1990294/range1990294/1

https://synbiohub.org/public/igem/BBa_K098010/annotation1990294/range1990294/1
type: Range
persistent identity: range1990294
display id: range1990294
version: 1
direction: direction/forward
top level: BBa_K098010
start: 630
end: 643
orientation: inline

BBa_B0015 (terminator)
type: SequenceAnnotation
persistent identity: annotation1990296
display id: annotation1990296
version: 1
top level: BBa_K098010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K098010/annotation1990296/range1990296/1

https://synbiohub.org/public/igem/BBa_K098010/annotation1990296/range1990296/1
type: Range
persistent identity: range1990296
display id: range1990296
version: 1
direction: direction/forward
top level: BBa_K098010
start: 1403
end: 1502
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1990292
display id: annotation1990292
version: 1
top level: BBa_K098010
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K098010/annotation1990292/range1990292/1

https://synbiohub.org/public/igem/BBa_K098010/annotation1990292/range1990292/1
type: Range
persistent identity: range1990292
display id: range1990292
version: 1
direction: direction/forward
top level: BBa_K098010
start: 44
end: 56
orientation: inline

BBa_J23113
type: SequenceAnnotation
persistent identity: annotation1990291
display id: annotation1990291
version: 1
top level: BBa_K098010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K098010/annotation1990291/range1990291/1

https://synbiohub.org/public/igem/BBa_K098010/annotation1990291/range1990291/1
type: Range
persistent identity: range1990291
display id: range1990291
version: 1
direction: direction/forward
top level: BBa_K098010
start: 1
end: 35
orientation: inline

HO
type: SequenceAnnotation
persistent identity: annotation1990293
display id: annotation1990293
version: 1
top level: BBa_K098010
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K098010/annotation1990293/range1990293/1

https://synbiohub.org/public/igem/BBa_K098010/annotation1990293/range1990293/1
type: Range
persistent identity: range1990293
display id: range1990293
version: 1
direction: direction/forward
top level: BBa_K098010
start: 63
end: 629
orientation: inline

pcyA
type: SequenceAnnotation
persistent identity: annotation1990295
display id: annotation1990295
version: 1
top level: BBa_K098010
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K098010/annotation1990295/range1990295/1

https://synbiohub.org/public/igem/BBa_K098010/annotation1990295/range1990295/1
type: Range
persistent identity: range1990295
display id: range1990295
version: 1
direction: direction/forward
top level: BBa_K098010
start: 650
end: 1396
orientation: inline

BBa_K338029
type: ComponentDefinition
persistent identity: BBa K338029
display id: BBa_K338029
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: +OmpR, +(CinR-HSL) Double Promoter
creator: Lucas Hartsough
created: 2010-10-26T11:00:00Z
modified: 2015-05-08T01:12:07Z
discontinued: false
dominant: false
group u list: _454_
m user id: 0
owner id: 6013
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: An intermediate part designed to be used with the AND gate designed by Caltech iGEM '10: [http://2010.igem.org/Team:Caltech/Project Project Details].
mutable notes: Designed to be placed upstream of positive feedback mechanisms and desired output genes.
mutable provenance: The Registry of Standard Biological Parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0082
component: BBa_R0077
sequence annotation: BBa_R0077
sequence annotation: BBa_R0082
sequence: https://synbiohub.org/public/igem/BBa_K338029_sequence/1

BBa_R0082
type: Component
persistent identity: component2104977
display id: component2104977
version: 1
top level: BBa_K338029
access: public
definition: OmpR

BBa_R0077
type: Component
persistent identity: component2104982
display id: component2104982
version: 1
top level: BBa_K338029
access: public
definition: https://synbiohub.org/public/igem/BBa_R0077/1

BBa_R0077
type: SequenceAnnotation
persistent identity: annotation2104982
display id: annotation2104982
version: 1
top level: BBa_K338029
location: https://synbiohub.org/public/igem/BBa_K338029/annotation2104982/range2104982/1
component: BBa_R0077

https://synbiohub.org/public/igem/BBa_K338029/annotation2104982/range2104982/1
type: Range
persistent identity: range2104982
display id: range2104982
version: 1
direction: direction/reverse
top level: BBa_K338029
start: 117
end: 347
orientation: inline

BBa_R0082
type: SequenceAnnotation
persistent identity: annotation2104977
display id: annotation2104977
version: 1
top level: BBa_K338029
location: https://synbiohub.org/public/igem/BBa_K338029/annotation2104977/range2104977/1
component: BBa_R0082

https://synbiohub.org/public/igem/BBa_K338029/annotation2104977/range2104977/1
type: Range
persistent identity: range2104977
display id: range2104977
version: 1
direction: direction/reverse
top level: BBa_K338029
start: 1
end: 108
orientation: inline

BBa_K098011
type: ComponentDefinition
persistent identity: BBa K098011
display id: BBa_K098011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: E. coli ompR, expressed at low level
creator: Harvard iGEM 08
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:08:41Z
discontinued: false
dominant: false
experience: experience/None
group u list: _196_
m user id: 0
owner id: 3389
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: *ompR, E. coli two-component signal transduction system used along with EnvZ *controls expression of genes under ompC and ompF promoters *doi:10.1016/j.physletb.2003.10.071
mutable notes: obtained by PCR
mutable provenance: E. coli genome (DH5alpha)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: BBa_B0015 (terminator)
sequence annotation: BBa_J23113
sequence annotation: ompR
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_K098011_sequence/1

BBa_B0015 (terminator)
type: SequenceAnnotation
persistent identity: annotation1990311
display id: annotation1990311
version: 1
top level: BBa_K098011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K098011/annotation1990311/range1990311/1

https://synbiohub.org/public/igem/BBa_K098011/annotation1990311/range1990311/1
type: Range
persistent identity: range1990311
display id: range1990311
version: 1
direction: direction/forward
top level: BBa_K098011
start: 789
end: 888
orientation: inline

BBa_J23113
type: SequenceAnnotation
persistent identity: annotation1990307
display id: annotation1990307
version: 1
top level: BBa_K098011
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K098011/annotation1990307/range1990307/1

https://synbiohub.org/public/igem/BBa_K098011/annotation1990307/range1990307/1
type: Range
persistent identity: range1990307
display id: range1990307
version: 1
direction: direction/forward
top level: BBa_K098011
start: 1
end: 35
orientation: inline

ompR
type: SequenceAnnotation
persistent identity: annotation1990310
display id: annotation1990310
version: 1
top level: BBa_K098011
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K098011/annotation1990310/range1990310/1

https://synbiohub.org/public/igem/BBa_K098011/annotation1990310/range1990310/1
type: Range
persistent identity: range1990310
display id: range1990310
version: 1
direction: direction/forward
top level: BBa_K098011
start: 63
end: 782
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1990309
display id: annotation1990309
version: 1
top level: BBa_K098011
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K098011/annotation1990309/range1990309/1

https://synbiohub.org/public/igem/BBa_K098011/annotation1990309/range1990309/1
type: Range
persistent identity: range1990309
display id: range1990309
version: 1
direction: direction/forward
top level: BBa_K098011
start: 44
end: 56
orientation: inline

BBa_K1660005
type: ComponentDefinition
persistent identity: BBa K1660005
display id: BBa_K1660005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP controlled by the PompR promoter
creator: Dai Yuanyi
created: 2015-09-07T11:00:00Z
modified: 2015-09-08T12:26:12Z
discontinued: false
dominant: false
group u list: _2078_
m user id: 26933
owner id: 26933
owning group id: 9
status: status/Planning
bookmark: false
mutable description: PompR is a OmpR-controlled promoter which can be positively regulated by phosphorylated OmpR. This promoter is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription. We insert PompR(BBa_R0082) sequences into the upstream region of rfp gene, which together is connected to vector PSB1C3 afterwards, and therefore we are able to observe the OmpR promoter efficiency through the control of light.
mutable notes: We constructed this biobrick through pcr.
mutable provenance: This part comes from the stock.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_R0082
component: BBa_K516032
sequence annotation: BBa_K516032
sequence annotation: BBa_R0082
sequence: https://synbiohub.org/public/igem/BBa_K1660005_sequence/1

BBa_R0082
type: Component
persistent identity: component2446043
display id: component2446043
version: 1
top level: BBa_K1660005
access: public
definition: OmpR

BBa_K516032
type: Component
persistent identity: component2446057
display id: component2446057
version: 1
top level: BBa_K1660005
access: public
definition: https://synbiohub.org/public/igem/BBa_K516032/1

BBa_K516032
type: SequenceAnnotation
persistent identity: annotation2446057
display id: annotation2446057
version: 1
top level: BBa_K1660005
location: https://synbiohub.org/public/igem/BBa_K1660005/annotation2446057/range2446057/1
component: BBa_K516032

https://synbiohub.org/public/igem/BBa_K1660005/annotation2446057/range2446057/1
type: Range
persistent identity: range2446057
display id: range2446057
version: 1
direction: direction/reverse
top level: BBa_K1660005
start: 117
end: 978
orientation: inline

BBa_R0082
type: SequenceAnnotation
persistent identity: annotation2446043
display id: annotation2446043
version: 1
top level: BBa_K1660005
location: https://synbiohub.org/public/igem/BBa_K1660005/annotation2446043/range2446043/1
component: BBa_R0082

https://synbiohub.org/public/igem/BBa_K1660005/annotation2446043/range2446043/1
type: Range
persistent identity: range2446043
display id: range2446043
version: 1
direction: direction/reverse
top level: BBa_K1660005
start: 1
end: 108
orientation: inline

BBa_I15010
type: ComponentDefinition
persistent identity: BBa I15010
display id: BBa_I15010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cph8 (Cph1/EnvZ fusion)
creator: Jeff Tabor
created: 2004-09-19T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _5_
m user id: 0
owner id: 88
owning group id: 7
sample status: In stock
status: status/Available
bookmark: true
mutable description: Chimeric Cph1 light receptor/EnvZ protein.
mutable notes: Silent mutation at base 108 (G-A) to remove PstI site
mutable provenance: Voigt Lab via Anselm Levskaya
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Cph8
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_I15010_sequence/1

Cph8
type: SequenceAnnotation
persistent identity: annotation1891613
display id: annotation1891613
version: 1
top level: BBa_I15010
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I15010/annotation1891613/range1891613/1

https://synbiohub.org/public/igem/BBa_I15010/annotation1891613/range1891613/1
type: Range
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Help:Barcodes
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persistent identity: annotation2214031
display id: annotation2214031
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role: SO:0000807
role: feature/barcode
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BBa_M30109
type: ComponentDefinition
persistent identity: BBa M30109
display id: BBa_M30109
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Light responsive system, dual regulation
creator: natalie kuldell
created: 2005-11-14T12:00:00Z
modified: 2015-05-08T01:13:59Z
discontinued: false
dominant: true
experience: experience/None
group u list: _45_1_
m user id: 0
owner id: 314
owning group id: 1
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Pbad regulated phycobilins (ho1 and pcyA) followed by Ptet regulated light sensor (Cph8). This should allow repressed Pbad to give low levels of accessory proteins and derepressed Ptet to crank out lots of light sensor protein
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
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component: BBa_B0010
component: BBa_I13453
component: BBa_I15008
component: BBa_B0012
component: BBa_R0040
component: BBa_I15009
component: BBa_I15010
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sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_I13453
sequence annotation: BBa_I15008
sequence annotation: BBa_R0040
sequence annotation: BBa_I15010
sequence annotation: BBa_I15009
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BBa_B0010
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display id: range1747756
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end: 1683
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BBa_B0034
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display id: annotation1747742
version: 1
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location: https://synbiohub.org/public/igem/BBa_M30109/annotation1747742/range1747742/1
component: BBa_B0034

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type: Range
persistent identity: range1747742
display id: range1747742
version: 1
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end: 150
orientation: inline

nickname
type: ComponentDefinition
persistent identity: BBa K1479004
display id: BBa_K1479004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Just for upload test
creator: ChenYB
created: 2014-09-30T11:00:00Z
modified: 2015-05-08T01:10:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1859_
m user id: 0
owner id: 21515
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is just for upload test. It is meaningless
mutable notes: NULL
mutable provenance: NULL
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: BBa_B0011
sequence annotation: T7 TE
sequence annotation: BBa_B1008
sequence annotation: polya
sequence annotation: stem_loop
sequence annotation: stop
sequence annotation: PolyA
sequence annotation: BBa_B0012
sequence annotation: BBa_K1479001
sequence annotation: BBa_B0010
sequence annotation: B1007
sequence annotation: BBa_B0014
sequence annotation: B1008
sequence annotation: 22
sequence annotation: BBa_B1007
sequence annotation: misc
sequence annotation: BBa_B0015
sequence annotation: modified thr terminator
sequence annotation: modified thr
sequence annotation: BBa_K1479002
sequence: https://synbiohub.org/public/igem/BBa_K1479004_sequence/1

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2397963
display id: annotation2397963
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397963/range2397963/1

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type: Range
persistent identity: range2397963
display id: range2397963
version: 1
direction: direction/forward
top level: nickname
start: 50
end: 95
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2397978
display id: annotation2397978
version: 1
top level: nickname
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397978/range2397978/1

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type: Range
persistent identity: range2397978
display id: range2397978
version: 1
direction: direction/forward
top level: nickname
start: 207
end: 226
orientation: inline

BBa_B1008
type: SequenceAnnotation
persistent identity: annotation2397967
display id: annotation2397967
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
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type: Range
persistent identity: range2397967
display id: range2397967
version: 1
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polya
type: SequenceAnnotation
persistent identity: annotation2397961
display id: annotation2397961
version: 1
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role: SO:0000553
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type: Range
persistent identity: range2397961
display id: range2397961
version: 1
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T7 TE
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persistent identity: annotation2397960
display id: annotation2397960
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role: SO:0000313
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display id: range2397960
version: 1
direction: direction/forward
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start: 8
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orientation: inline

stem_loop
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persistent identity: annotation2397985
display id: annotation2397985
version: 1
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role: feature/stem loop
role: SO:0000313
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type: Range
persistent identity: range2397985
display id: range2397985
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top level: nickname
start: 314
end: 357
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2397962
display id: annotation2397962
version: 1
top level: nickname
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397962/range2397962/1

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type: Range
persistent identity: range2397962
display id: range2397962
version: 1
direction: direction/forward
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start: 34
end: 34
orientation: inline

PolyA
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display id: annotation2397968
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type: Range
persistent identity: range2397968
display id: range2397968
version: 1
direction: direction/reverse
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start: 108
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orientation: inline

BBa_B0012
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display id: annotation2397959
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role: feature/BioBrick
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display id: range2397959
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polya
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display id: range2397988
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BBa_K1479001
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BBa_B0012
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BBa_B0010
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type: Range
persistent identity: range2397982
display id: range2397982
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orientation: inline

PolyA
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type: Range
persistent identity: range2397970
display id: range2397970
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direction: direction/forward
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start: 135
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orientation: inline

stop
type: SequenceAnnotation
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display id: range2397980
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PolyA
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B1007
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display id: annotation2397972
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role: feature/BioBrick
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type: Range
persistent identity: range2397972
display id: range2397972
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direction: direction/forward
top level: nickname
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end: 191
orientation: inline

BBa_B0014
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display id: range2397977
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orientation: inline

B1008
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display id: annotation2397966
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role: SO:0000804
role: feature/BioBrick
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type: Range
persistent identity: range2397966
display id: range2397966
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direction: direction/forward
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orientation: inline

22
type: SequenceAnnotation
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type: Range
persistent identity: range2397957
display id: range2397957
version: 1
direction: direction/forward
top level: nickname
start: 1
end: 10
orientation: inline

BBa_B1007
type: SequenceAnnotation
persistent identity: annotation2397971
display id: annotation2397971
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397971/range2397971/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397971/range2397971/1
type: Range
persistent identity: range2397971
display id: range2397971
version: 1
direction: direction/reverse
top level: nickname
start: 152
end: 191
orientation: inline

misc
type: SequenceAnnotation
persistent identity: annotation2397956
display id: annotation2397956
version: 1
top level: nickname
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397956/range2397956/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397956/range2397956/1
type: Range
persistent identity: range2397956
display id: range2397956
version: 1
direction: direction/forward
top level: nickname
start: 1
end: 20
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2397984
display id: annotation2397984
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397984/range2397984/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397984/range2397984/1
type: Range
persistent identity: range2397984
display id: range2397984
version: 1
direction: direction/reverse
top level: nickname
start: 303
end: 431
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2397981
display id: annotation2397981
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397981/range2397981/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397981/range2397981/1
type: Range
persistent identity: range2397981
display id: range2397981
version: 1
direction: direction/forward
top level: nickname
start: 249
end: 294
orientation: inline

misc
type: SequenceAnnotation
persistent identity: annotation2397990
display id: annotation2397990
version: 1
top level: nickname
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397990/range2397990/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397990/range2397990/1
type: Range
persistent identity: range2397990
display id: range2397990
version: 1
direction: direction/reverse
top level: nickname
start: 1
end: 20
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2397979
display id: annotation2397979
version: 1
top level: nickname
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397979/range2397979/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397979/range2397979/1
type: Range
persistent identity: range2397979
display id: range2397979
version: 1
direction: direction/forward
top level: nickname
start: 227
end: 240
orientation: inline

modified thr terminator
type: SequenceAnnotation
persistent identity: annotation2397969
display id: annotation2397969
version: 1
top level: nickname
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397969/range2397969/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397969/range2397969/1
type: Range
persistent identity: range2397969
display id: range2397969
version: 1
direction: direction/forward
top level: nickname
start: 113
end: 134
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2397964
display id: annotation2397964
version: 1
top level: nickname
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397964/range2397964/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397964/range2397964/1
type: Range
persistent identity: range2397964
display id: range2397964
version: 1
direction: direction/forward
top level: nickname
start: 62
end: 84
orientation: inline

modified thr
type: SequenceAnnotation
persistent identity: annotation2397974
display id: annotation2397974
version: 1
top level: nickname
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397974/range2397974/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397974/range2397974/1
type: Range
persistent identity: range2397974
display id: range2397974
version: 1
direction: direction/forward
top level: nickname
start: 161
end: 182
orientation: inline

BBa_K1479002
type: SequenceAnnotation
persistent identity: annotation2397955
display id: annotation2397955
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397955/range2397955/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397955/range2397955/1
type: Range
persistent identity: range2397955
display id: range2397955
version: 1
direction: direction/reverse
top level: nickname
start: 1
end: 95
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2397986
display id: annotation2397986
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397986/range2397986/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397986/range2397986/1
type: Range
persistent identity: range2397986
display id: range2397986
version: 1
direction: direction/forward
top level: nickname
start: 391
end: 431
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2397989
display id: annotation2397989
version: 1
top level: nickname
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397989/range2397989/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397989/range2397989/1
type: Range
persistent identity: range2397989
display id: range2397989
version: 1
direction: direction/forward
top level: nickname
start: 424
end: 424
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2397987
display id: annotation2397987
version: 1
top level: nickname
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397987/range2397987/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397987/range2397987/1
type: Range
persistent identity: range2397987
display id: range2397987
version: 1
direction: direction/forward
top level: nickname
start: 398
end: 417
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation2397975
display id: annotation2397975
version: 1
top level: nickname
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397975/range2397975/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397975/range2397975/1
type: Range
persistent identity: range2397975
display id: range2397975
version: 1
direction: direction/forward
top level: nickname
start: 183
end: 188
orientation: inline

BBa_K1587008
type: ComponentDefinition
persistent identity: BBa K1587008
display id: BBa_K1587008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Red light biosensor
creator: Alexandre Le Scornet
created: 2015-09-15T11:00:00Z
modified: 2015-09-17T02:51:05Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _2004_
m user id: 24598
owner id: 24598
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Strong promoter BBa_J23119. Chimeric Red light receptor cph8 BBa_I15010 (cph1-envZ) and proteins involved in phycobilin biosynthesis ho1 (BBa_I15008), pcyA (BBa_I15009).
mutable notes: No information
mutable provenance: Sequences are from Cyanobacteria : - Cph1 domain - ho1 BBa_I15008 - pcyA BBa_I15009
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: rbs
sequence annotation: Strong promoter
sequence annotation: cph8
sequence: https://synbiohub.org/public/igem/BBa_K1587008_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation2466981
display id: annotation2466981
version: 1
top level: BBa_K1587008
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1587008/annotation2466981/range2466981/1

https://synbiohub.org/public/igem/BBa_K1587008/annotation2466981/range2466981/1
type: Range
persistent identity: range2466981
display id: range2466981
version: 1
direction: direction/forward
top level: BBa_K1587008
start: 36
end: 50
orientation: inline

Strong promoter
type: SequenceAnnotation
persistent identity: annotation2466980
display id: annotation2466980
version: 1
top level: BBa_K1587008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1587008/annotation2466980/range2466980/1

https://synbiohub.org/public/igem/BBa_K1587008/annotation2466980/range2466980/1
type: Range
persistent identity: range2466980
display id: range2466980
version: 1
direction: direction/forward
top level: BBa_K1587008
start: 1
end: 35
orientation: inline

cph8
type: SequenceAnnotation
persistent identity: annotation2466982
display id: annotation2466982
version: 1
top level: BBa_K1587008
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1587008/annotation2466982/range2466982/1

https://synbiohub.org/public/igem/BBa_K1587008/annotation2466982/range2466982/1
type: Range
persistent identity: range2466982
display id: range2466982
version: 1
direction: direction/forward
top level: BBa_K1587008
start: 51
end: 2288
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS (Elowitz 1999) -- defines RBS efficiency
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: RBS based on Elowitz repressilator.
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
direction: direction/forward
top level: BBa_B0034
start: 5
end: 8
orientation: inline

BBa_I15008
type: ComponentDefinition
persistent identity: BBa I15008
display id: BBa_I15008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: heme oxygenase (ho1) from Synechocystis
creator: Jeff Tabor
created: 2004-09-19T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _5_
m user id: 0
owner id: 88
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: One of two requisite genes required for the biosynthesis of phycocyanobilin from heme.
mutable provenance: Voigt Lab via Anselm Levskaya
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_I15008_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214026
display id: annotation2214026
version: 1
top level: BBa_I15008
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_I15008/annotation2214026/range2214026/1

https://synbiohub.org/public/igem/BBa_I15008/annotation2214026/range2214026/1
type: Range
persistent identity: range2214026
display id: range2214026
version: 1
direction: direction/forward
top level: BBa_I15008
start: 727
end: 751
orientation: inline

BBa_I15009
type: ComponentDefinition
persistent identity: BBa I15009
display id: BBa_I15009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: phycocyanobilin:ferredoxin oxidoreductase (PcyA) from synechocystis
creator: Jeff Tabor
created: 2004-09-19T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _5_
m user id: 0
owner id: 88
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Second of two required phycocyanobilin biosynthetic genes.
mutable provenance: Voigt Lab via Anselm Levskaya
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: PcyA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_I15009_sequence/1

PcyA
type: SequenceAnnotation
persistent identity: annotation1891612
display id: annotation1891612
version: 1
top level: BBa_I15009
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I15009/annotation1891612/range1891612/1

https://synbiohub.org/public/igem/BBa_I15009/annotation1891612/range1891612/1
type: Range
persistent identity: range1891612
display id: range1891612
version: 1
direction: direction/forward
top level: BBa_I15009
start: 1
end: 750
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214032
display id: annotation2214032
version: 1
top level: BBa_I15009
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_I15009/annotation2214032/range2214032/1

https://synbiohub.org/public/igem/BBa_I15009/annotation2214032/range2214032/1
type: Range
persistent identity: range2214032
display id: range2214032
version: 1
direction: direction/forward
top level: BBa_I15009
start: 751
end: 775
orientation: inline

lacZ a
type: ComponentDefinition
persistent identity: BBa E0033
display id: BBa_E0033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacZ alpha fragment; complements matching N-terminal deletion mutant (lacZ-omega)
creator: Yong-Su Jin (Fighting Darwins)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:25Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable description: to greatly reduce the number of bases, this is only a portion of the LacZ gene
mutable notes: Restriction sites (modified) 76-79 (gcc to gca) 79-81 (gct to gca) 85-87 (gaa to gag) 106-108 (tgc to tgt) 109-111(agg to aga)
mutable provenance: www.ncbi.nlm.nih.gov
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: T7 promoter
sequence annotation: A
sequence annotation: C
sequence annotation: lacZ alpha
sequence annotation: lacZ gene fragment
sequence annotation: T3 promoter
sequence annotation: G
sequence annotation: T
sequence: https://synbiohub.org/public/igem/BBa_E0033_sequence/1

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1938940
display id: annotation1938940
version: 1
top level: lacZ a
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_E0033/annotation1938940/range1938940/1

https://synbiohub.org/public/igem/BBa_E0033/annotation1938940/range1938940/1
type: Range
persistent identity: range1938940
display id: range1938940
version: 1
direction: direction/reverse
top level: lacZ a
start: 173
end: 191
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation308381
display id: annotation308381
version: 1
top level: lacZ a
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_E0033/annotation308381/range308381/1

https://synbiohub.org/public/igem/BBa_E0033/annotation308381/range308381/1
type: Range
persistent identity: range308381
display id: range308381
version: 1
direction: direction/forward
top level: lacZ a
start: 87
end: 87
orientation: inline

C
type: SequenceAnnotation
persistent identity: annotation308387
display id: annotation308387
version: 1
top level: lacZ a
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_E0033/annotation308387/range308387/1

https://synbiohub.org/public/igem/BBa_E0033/annotation308387/range308387/1
type: Range
persistent identity: range308387
display id: range308387
version: 1
direction: direction/forward
top level: lacZ a
start: 78
end: 78
orientation: inline

lacZ alpha
type: SequenceAnnotation
persistent identity: annotation308320
display id: annotation308320
version: 1
top level: lacZ a
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_E0033/annotation308320/range308320/1

https://synbiohub.org/public/igem/BBa_E0033/annotation308320/range308320/1
type: Range
persistent identity: range308320
display id: range308320
version: 1
direction: direction/forward
top level: lacZ a
start: 1
end: 348
orientation: inline

lacZ gene fragment
type: SequenceAnnotation
persistent identity: annotation1938942
display id: annotation1938942
version: 1
top level: lacZ a
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_E0033/annotation1938942/range1938942/1

https://synbiohub.org/public/igem/BBa_E0033/annotation1938942/range1938942/1
type: Range
persistent identity: range1938942
display id: range1938942
version: 1
direction: direction/forward
top level: lacZ a
start: 1
end: 15
orientation: inline

T3 promoter
type: SequenceAnnotation
persistent identity: annotation1938939
display id: annotation1938939
version: 1
top level: lacZ a
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_E0033/annotation1938939/range1938939/1

https://synbiohub.org/public/igem/BBa_E0033/annotation1938939/range1938939/1
type: Range
persistent identity: range1938939
display id: range1938939
version: 1
direction: direction/forward
top level: lacZ a
start: 26
end: 35
orientation: inline

lacZ gene fragment
type: SequenceAnnotation
persistent identity: annotation1938941
display id: annotation1938941
version: 1
top level: lacZ a
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_E0033/annotation1938941/range1938941/1

https://synbiohub.org/public/igem/BBa_E0033/annotation1938941/range1938941/1
type: Range
persistent identity: range1938941
display id: range1938941
version: 1
direction: direction/forward
top level: lacZ a
start: 199
end: 348
orientation: inline

C
type: SequenceAnnotation
persistent identity: annotation308383
display id: annotation308383
version: 1
top level: lacZ a
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_E0033/annotation308383/range308383/1

https://synbiohub.org/public/igem/BBa_E0033/annotation308383/range308383/1
type: Range
persistent identity: range308383
display id: range308383
version: 1
direction: direction/forward
top level: lacZ a
start: 108
end: 108
orientation: inline

G
type: SequenceAnnotation
persistent identity: annotation308388
display id: annotation308388
version: 1
top level: lacZ a
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_E0033/annotation308388/range308388/1

https://synbiohub.org/public/igem/BBa_E0033/annotation308388/range308388/1
type: Range
persistent identity: range308388
display id: range308388
version: 1
direction: direction/forward
top level: lacZ a
start: 111
end: 111
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation308375
display id: annotation308375
version: 1
top level: lacZ a
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_E0033/annotation308375/range308375/1

https://synbiohub.org/public/igem/BBa_E0033/annotation308375/range308375/1
type: Range
persistent identity: range308375
display id: range308375
version: 1
direction: direction/forward
top level: lacZ a
start: 81
end: 81
orientation: inline

BBa_K1660004
type: ComponentDefinition
persistent identity: BBa K1660004
display id: BBa_K1660004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PcyA+Ho1
creator: Dai Yuanyi
created: 2015-09-06T11:00:00Z
modified: 2015-09-07T11:31:12Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2078_
m user id: 26933
owner id: 26933
owning group id: 9
status: status/Planning
bookmark: true
mutable description: In order to work,Cph8 has to form chromophore with PCB biosynthetic genes, where the formation of PCB requires the gene pcyA and hol to function as accounted below. Hol is a sort of Iron red pigment oxidase which can oxidize the heme group using a ferredoxin cofactor, generating biliverdin IXalpha. And then, PcyA, a kind of ferredoxin oxidase from Synechocystis Sauv, functions to turn biliverdin IXalpha (BV) into PCB. We connected gene pcyA（BBa_I15009）and ho1（BBa_I15008）together along with the constitutive promoter(BBa_J23100) to ensure a continuous work of the photoinduction system in our E.coli.
mutable notes: We used oe-pcr to assemble these parts together seamlessly.
mutable provenance: pcyA gene and ho1 gene both come from Synechocystis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: stop codon
sequence annotation: ho1 barcode
sequence annotation: pcyA barcode
sequence annotation: J23100
sequence annotation: start codon
sequence annotation: ho1
sequence annotation: B0034
sequence annotation: pcyA
sequence: https://synbiohub.org/public/igem/BBa_K1660004_sequence/1

stop codon
type: SequenceAnnotation
persistent identity: annotation2445868
display id: annotation2445868
version: 1
top level: BBa_K1660004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1660004/annotation2445868/range2445868/1

https://synbiohub.org/public/igem/BBa_K1660004/annotation2445868/range2445868/1
type: Range
persistent identity: range2445868
display id: range2445868
version: 1
direction: direction/forward
top level: BBa_K1660004
start: 792
end: 797
orientation: inline

ho1 barcode
type: SequenceAnnotation
persistent identity: annotation2445942
display id: annotation2445942
version: 1
top level: BBa_K1660004
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_K1660004/annotation2445942/range2445942/1

https://synbiohub.org/public/igem/BBa_K1660004/annotation2445942/range2445942/1
type: Range
persistent identity: range2445942
display id: range2445942
version: 1
direction: direction/forward
top level: BBa_K1660004
start: 1561
end: 1585
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation2445943
display id: annotation2445943
version: 1
top level: BBa_K1660004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1660004/annotation2445943/range2445943/1

https://synbiohub.org/public/igem/BBa_K1660004/annotation2445943/range2445943/1
type: Range
persistent identity: range2445943
display id: range2445943
version: 1
direction: direction/forward
top level: BBa_K1660004
start: 1558
end: 1560
orientation: inline

pcyA barcode
type: SequenceAnnotation
persistent identity: annotation2445869
display id: annotation2445869
version: 1
top level: BBa_K1660004
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_K1660004/annotation2445869/range2445869/1

https://synbiohub.org/public/igem/BBa_K1660004/annotation2445869/range2445869/1
type: Range
persistent identity: range2445869
display id: range2445869
version: 1
direction: direction/forward
top level: BBa_K1660004
start: 798
end: 822
orientation: inline

J23100
type: SequenceAnnotation
persistent identity: annotation2445845
display id: annotation2445845
version: 1
top level: BBa_K1660004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1660004/annotation2445845/range2445845/1

https://synbiohub.org/public/igem/BBa_K1660004/annotation2445845/range2445845/1
type: Range
persistent identity: range2445845
display id: range2445845
version: 1
direction: direction/forward
top level: BBa_K1660004
start: 1
end: 35
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2445865
display id: annotation2445865
version: 1
top level: BBa_K1660004
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1660004/annotation2445865/range2445865/1

https://synbiohub.org/public/igem/BBa_K1660004/annotation2445865/range2445865/1
type: Range
persistent identity: range2445865
display id: range2445865
version: 1
direction: direction/forward
top level: BBa_K1660004
start: 48
end: 50
orientation: inline

ho1
type: SequenceAnnotation
persistent identity: annotation2445885
display id: annotation2445885
version: 1
top level: BBa_K1660004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1660004/annotation2445885/range2445885/1

https://synbiohub.org/public/igem/BBa_K1660004/annotation2445885/range2445885/1
type: Range
persistent identity: range2445885
display id: range2445885
version: 1
direction: direction/forward
top level: BBa_K1660004
start: 835
end: 1560
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2445938
display id: annotation2445938
version: 1
top level: BBa_K1660004
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1660004/annotation2445938/range2445938/1

https://synbiohub.org/public/igem/BBa_K1660004/annotation2445938/range2445938/1
type: Range
persistent identity: range2445938
display id: range2445938
version: 1
direction: direction/forward
top level: BBa_K1660004
start: 835
end: 837
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2445864
display id: annotation2445864
version: 1
top level: BBa_K1660004
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1660004/annotation2445864/range2445864/1

https://synbiohub.org/public/igem/BBa_K1660004/annotation2445864/range2445864/1
type: Range
persistent identity: range2445864
display id: range2445864
version: 1
direction: direction/forward
top level: BBa_K1660004
start: 36
end: 47
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2445884
display id: annotation2445884
version: 1
top level: BBa_K1660004
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1660004/annotation2445884/range2445884/1

https://synbiohub.org/public/igem/BBa_K1660004/annotation2445884/range2445884/1
type: Range
persistent identity: range2445884
display id: range2445884
version: 1
direction: direction/forward
top level: BBa_K1660004
start: 823
end: 834
orientation: inline

pcyA
type: SequenceAnnotation
persistent identity: annotation2445867
display id: annotation2445867
version: 1
top level: BBa_K1660004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1660004/annotation2445867/range2445867/1

https://synbiohub.org/public/igem/BBa_K1660004/annotation2445867/range2445867/1
type: Range
persistent identity: range2445867
display id: range2445867
version: 1
direction: direction/forward
top level: BBa_K1660004
start: 48
end: 750
orientation: inline

OmpR
type: ComponentDefinition
persistent identity: BBa R0082
display id: BBa_R0082
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (OmpR, positive)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Positively regulated, OmpR-controlled promoter. This promoter is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription.
mutable notes: The promoter includes the three OmpR binding sites: C1, C2, and C3. The promoter starts at -107 and goes up to the transcriptional start site at +1. An alternate version, BBa_R0083, cuts out the C2 and C3 sites. The efficacy of this promoter versus the truncated ompC upstream region (BBa_R0083) or the ompF upstream region (BBa_R0084) is currently unknown.
mutable provenance: NC_000193 E. coli K12
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: -35
sequence annotation: C1 OmpR
sequence annotation: C2 OmpR
sequence annotation: C3 OmpR
sequence: https://synbiohub.org/public/igem/BBa_R0082_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation301167
display id: annotation301167
version: 1
top level: OmpR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301167/range301167/1

https://synbiohub.org/public/igem/BBa_R0082/annotation301167/range301167/1
type: Range
persistent identity: range301167
display id: range301167
version: 1
direction: direction/forward
top level: OmpR
start: 98
end: 103
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation301166
display id: annotation301166
version: 1
top level: OmpR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301166/range301166/1

https://synbiohub.org/public/igem/BBa_R0082/annotation301166/range301166/1
type: Range
persistent identity: range301166
display id: range301166
version: 1
direction: direction/forward
top level: OmpR
start: 75
end: 80
orientation: inline

C1 OmpR
type: SequenceAnnotation
persistent identity: annotation301154
display id: annotation301154
version: 1
top level: OmpR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301154/range301154/1

https://synbiohub.org/public/igem/BBa_R0082/annotation301154/range301154/1
type: Range
persistent identity: range301154
display id: range301154
version: 1
direction: direction/forward
top level: OmpR
start: 13
end: 30
orientation: inline

C2 OmpR
type: SequenceAnnotation
persistent identity: annotation301155
display id: annotation301155
version: 1
top level: OmpR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301155/range301155/1

https://synbiohub.org/public/igem/BBa_R0082/annotation301155/range301155/1
type: Range
persistent identity: range301155
display id: range301155
version: 1
direction: direction/forward
top level: OmpR
start: 34
end: 51
orientation: inline

C3 OmpR
type: SequenceAnnotation
persistent identity: annotation301156
display id: annotation301156
version: 1
top level: OmpR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301156/range301156/1

https://synbiohub.org/public/igem/BBa_R0082/annotation301156/range301156/1
type: Range
persistent identity: range301156
display id: range301156
version: 1
direction: direction/forward
top level: OmpR
start: 54
end: 71
orientation: inline