//function/cellsignalling/luxr
type: Collection
persistent identity: function cellsignalling luxr
display id: function_cellsignalling_luxr
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K266005
member: BBa_J102001
member: BBa_I751502
member: luxR
member: BBa_K091157
member: BBa_I739105
member: BBa_K266006
member: BBa_K1520515
member: lux pR
member: lux pL
member: BBa_J64712
member: BBa_K091156
member: lux pR-3
member: BBa_K091107
member: BBa_J64067
member: BBa_K1520516
member: BBa_K266007
member: lux pR-5
member: Lux
member: BBa_K091146
member: BBa_K658008
member: BBa_I751501
member: BBa_K145150
member: BBa_K1520010

BBa_K091146
type: ComponentDefinition
persistent identity: BBa K091146
display id: BBa_K091146
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLas/Lux Hybrid Promoter
creator: Erin Feeney
created: 2008-07-16T11:00:00Z
modified: 2015-05-08T01:08:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3067
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: K091146 is a modified version of the pLas' promoter (BBa_K091117) containing the addition of a luxR binding site between the -35 and -10 regions. This promoter is expected to be activated in the presence of PAI-1 and LasR and repressed in the presence of AI-1 and LuxR.
mutable notes: The Lux box was placed adjacent to the LasR binding site with the aim of minimizing cross-talk in the presence of both PAI-1 and AI-1; since the LasR site should have a higher affinity, we hope that the binding of PAI-1 will competitively exclude the binding of AI-1.
mutable provenance: The LuxR binding site is from BBa_R0062 and the pLas' promoter is from the region upstream of the LasI gene in Pseudomonas aeruginosa.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: LasR
sequence annotation: LuxR
sequence annotation: Transcriptional Start
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_K091146_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1967356
display id: annotation1967356
version: 1
top level: BBa_K091146
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K091146/annotation1967356/range1967356/1

https://synbiohub.org/public/igem/BBa_K091146/annotation1967356/range1967356/1
type: Range
persistent identity: range1967356
display id: range1967356
version: 1
direction: direction/forward
top level: BBa_K091146
start: 113
end: 118
orientation: inline

LasR
type: SequenceAnnotation
persistent identity: annotation1967354
display id: annotation1967354
version: 1
top level: BBa_K091146
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K091146/annotation1967354/range1967354/1

https://synbiohub.org/public/igem/BBa_K091146/annotation1967354/range1967354/1
type: Range
persistent identity: range1967354
display id: range1967354
version: 1
direction: direction/forward
top level: BBa_K091146
start: 75
end: 93
orientation: inline

LuxR
type: SequenceAnnotation
persistent identity: annotation1967355
display id: annotation1967355
version: 1
top level: BBa_K091146
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K091146/annotation1967355/range1967355/1

https://synbiohub.org/public/igem/BBa_K091146/annotation1967355/range1967355/1
type: Range
persistent identity: range1967355
display id: range1967355
version: 1
direction: direction/forward
top level: BBa_K091146
start: 94
end: 113
orientation: inline

Transcriptional Start
type: SequenceAnnotation
persistent identity: annotation1967358
display id: annotation1967358
version: 1
top level: BBa_K091146
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K091146/annotation1967358/range1967358/1

https://synbiohub.org/public/igem/BBa_K091146/annotation1967358/range1967358/1
type: Range
persistent identity: range1967358
display id: range1967358
version: 1
direction: direction/forward
top level: BBa_K091146
start: 125
end: 125
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1967357
display id: annotation1967357
version: 1
top level: BBa_K091146
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K091146/annotation1967357/range1967357/1

https://synbiohub.org/public/igem/BBa_K091146/annotation1967357/range1967357/1
type: Range
persistent identity: range1967357
display id: range1967357
version: 1
direction: direction/forward
top level: BBa_K091146
start: 86
end: 91
orientation: inline

BBa_K1520516
type: ComponentDefinition
persistent identity: BBa K1520516
display id: BBa_K1520516
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MC31-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-OMPA-golB-rbs-luxI-Ter
creator: yue wu
created: 2014-09-22T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: MC31-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-OMPA-golB-rbs-luxI-Ter It is a test of our positive feedback circuit for microcystin.
mutable notes: None
mutable provenance: MC31, luxI, Pcons2, luxR, Prlux, OMPA-golB, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520029
component: BBa_K1520504
component: BBa_K1520003
component: BBa_K1520002
component: BBa_R0062
component: BBa_K1520507
sequence annotation: BBa_K1520504
sequence annotation: BBa_K1520002
sequence annotation: BBa_R0062
sequence annotation: BBa_K1520029
sequence annotation: BBa_K1520507
sequence annotation: BBa_K1520003
sequence: https://synbiohub.org/public/igem/BBa_K1520516_sequence/1

BBa_K1520029
type: Component
persistent identity: component2385696
display id: component2385696
version: 1
top level: BBa_K1520516
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_K1520029
type: Component
persistent identity: component2385718
display id: component2385718
version: 1
top level: BBa_K1520516
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_K1520504
type: Component
persistent identity: component2385717
display id: component2385717
version: 1
top level: BBa_K1520516
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520504/1

BBa_K1520003
type: Component
persistent identity: component2385702
display id: component2385702
version: 1
top level: BBa_K1520516
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520003/1

BBa_K1520002
type: Component
persistent identity: component2385692
display id: component2385692
version: 1
top level: BBa_K1520516
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520002/1

BBa_K1520504
type: Component
persistent identity: component2385695
display id: component2385695
version: 1
top level: BBa_K1520516
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520504/1

BBa_R0062
type: Component
persistent identity: component2385704
display id: component2385704
version: 1
top level: BBa_K1520516
access: public
definition: lux pR

BBa_K1520507
type: Component
persistent identity: component2385714
display id: component2385714
version: 1
top level: BBa_K1520516
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520507/1

BBa_K1520504
type: SequenceAnnotation
persistent identity: annotation2385695
display id: annotation2385695
version: 1
top level: BBa_K1520516
location: https://synbiohub.org/public/igem/BBa_K1520516/annotation2385695/range2385695/1
component: BBa_K1520504

https://synbiohub.org/public/igem/BBa_K1520516/annotation2385695/range2385695/1
type: Range
persistent identity: range2385695
display id: range2385695
version: 1
direction: direction/reverse
top level: BBa_K1520516
start: 141
end: 801
orientation: inline

BBa_K1520002
type: SequenceAnnotation
persistent identity: annotation2385692
display id: annotation2385692
version: 1
top level: BBa_K1520516
location: https://synbiohub.org/public/igem/BBa_K1520516/annotation2385692/range2385692/1
component: BBa_K1520002

https://synbiohub.org/public/igem/BBa_K1520516/annotation2385692/range2385692/1
type: Range
persistent identity: range2385692
display id: range2385692
version: 1
direction: direction/reverse
top level: BBa_K1520516
start: 1
end: 132
orientation: inline

BBa_K1520504
type: SequenceAnnotation
persistent identity: annotation2385717
display id: annotation2385717
version: 1
top level: BBa_K1520516
location: https://synbiohub.org/public/igem/BBa_K1520516/annotation2385717/range2385717/1
component: BBa_K1520504

https://synbiohub.org/public/igem/BBa_K1520516/annotation2385717/range2385717/1
type: Range
persistent identity: range2385717
display id: range2385717
version: 1
direction: direction/reverse
top level: BBa_K1520516
start: 2813
end: 3473
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2385704
display id: annotation2385704
version: 1
top level: BBa_K1520516
location: https://synbiohub.org/public/igem/BBa_K1520516/annotation2385704/range2385704/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K1520516/annotation2385704/range2385704/1
type: Range
persistent identity: range2385704
display id: range2385704
version: 1
direction: direction/forward
top level: BBa_K1520516
start: 1934
end: 1988
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2385718
display id: annotation2385718
version: 1
top level: BBa_K1520516
location: https://synbiohub.org/public/igem/BBa_K1520516/annotation2385718/range2385718/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520516/annotation2385718/range2385718/1
type: Range
persistent identity: range2385718
display id: range2385718
version: 1
direction: direction/reverse
top level: BBa_K1520516
start: 3482
end: 3610
orientation: inline

BBa_K1520507
type: SequenceAnnotation
persistent identity: annotation2385714
display id: annotation2385714
version: 1
top level: BBa_K1520516
location: https://synbiohub.org/public/igem/BBa_K1520516/annotation2385714/range2385714/1
component: BBa_K1520507

https://synbiohub.org/public/igem/BBa_K1520516/annotation2385714/range2385714/1
type: Range
persistent identity: range2385714
display id: range2385714
version: 1
direction: direction/reverse
top level: BBa_K1520516
start: 1995
end: 2804
orientation: inline

BBa_K1520003
type: SequenceAnnotation
persistent identity: annotation2385702
display id: annotation2385702
version: 1
top level: BBa_K1520516
location: https://synbiohub.org/public/igem/BBa_K1520516/annotation2385702/range2385702/1
component: BBa_K1520003

https://synbiohub.org/public/igem/BBa_K1520516/annotation2385702/range2385702/1
type: Range
persistent identity: range2385702
display id: range2385702
version: 1
direction: direction/reverse
top level: BBa_K1520516
start: 947
end: 1925
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2385696
display id: annotation2385696
version: 1
top level: BBa_K1520516
location: https://synbiohub.org/public/igem/BBa_K1520516/annotation2385696/range2385696/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520516/annotation2385696/range2385696/1
type: Range
persistent identity: range2385696
display id: range2385696
version: 1
direction: direction/reverse
top level: BBa_K1520516
start: 810
end: 938
orientation: inline

BBa_J64712
type: ComponentDefinition
persistent identity: BBa J64712
display id: BBa_J64712
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LasR/LasI Inducible & RHLR/RHLI repressible Promoter
creator: laura lavery
created: 2007-03-24T12:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1431
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is similar to BBa_R0079 but has an added binding site for RHLR/RHLI in between the -35 and -10 region. Thus this promoter is induced by LasR/LasI but repressed by RHLR/RHLI binding.
mutable notes: The natural spacer between the -35 and -10 is 14bp and the natural O1 site for RHLR/RHLI is 20bp long. There was not much over lap of the -35 or the -10 with this sequence, so it was nessicary to chop off some of the binding site sequence in order to maintain the correct spacing and preserve the -35 and -10 sequence. The binding site sequence is missing a tcc on is 5' end and a t on its 3' end.
mutable provenance: Rust L. et. al., "Analysis of the Pseudomonas aeruginosa Elastase (lasB) Regulatory Region". Journal of Bacteriology, 1996.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: -35
sequence annotation: LasR/LasI O2
sequence annotation: RHLR/RHLI
sequence annotation: LasR/LasI O1
sequence: https://synbiohub.org/public/igem/BBa_J64712_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1921068
display id: annotation1921068
version: 1
top level: BBa_J64712
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64712/annotation1921068/range1921068/1

https://synbiohub.org/public/igem/BBa_J64712/annotation1921068/range1921068/1
type: Range
persistent identity: range1921068
display id: range1921068
version: 1
direction: direction/forward
top level: BBa_J64712
start: 140
end: 145
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1921067
display id: annotation1921067
version: 1
top level: BBa_J64712
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64712/annotation1921067/range1921067/1

https://synbiohub.org/public/igem/BBa_J64712/annotation1921067/range1921067/1
type: Range
persistent identity: range1921067
display id: range1921067
version: 1
direction: direction/forward
top level: BBa_J64712
start: 117
end: 122
orientation: inline

LasR/LasI O2
type: SequenceAnnotation
persistent identity: annotation1921064
display id: annotation1921064
version: 1
top level: BBa_J64712
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J64712/annotation1921064/range1921064/1

https://synbiohub.org/public/igem/BBa_J64712/annotation1921064/range1921064/1
type: Range
persistent identity: range1921064
display id: range1921064
version: 1
direction: direction/forward
top level: BBa_J64712
start: 46
end: 64
orientation: inline

RHLR/RHLI
type: SequenceAnnotation
persistent identity: annotation1921066
display id: annotation1921066
version: 1
top level: BBa_J64712
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J64712/annotation1921066/range1921066/1

https://synbiohub.org/public/igem/BBa_J64712/annotation1921066/range1921066/1
type: Range
persistent identity: range1921066
display id: range1921066
version: 1
direction: direction/forward
top level: BBa_J64712
start: 125
end: 141
orientation: inline

LasR/LasI O1
type: SequenceAnnotation
persistent identity: annotation1921065
display id: annotation1921065
version: 1
top level: BBa_J64712
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J64712/annotation1921065/range1921065/1

https://synbiohub.org/public/igem/BBa_J64712/annotation1921065/range1921065/1
type: Range
persistent identity: range1921065
display id: range1921065
version: 1
direction: direction/forward
top level: BBa_J64712
start: 106
end: 125
orientation: inline

BBa_K091157
type: ComponentDefinition
persistent identity: BBa K091157
display id: BBa_K091157
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLux/Las Hybrid Promoter
creator: Erin Feeney
created: 2008-07-24T11:00:00Z
modified: 2015-05-08T01:08:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3067
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: K091157 is a modified version of the pLux' promoter (BBa_K091156) containing the addition of a LasR binding site between the -35 and -10 regions. This promoter is expected to be activated in the presence of AI-1 and LuxR and repressed in the presence of PAI-1 and LasR.
mutable notes: In order to fit the LasR binding site between the -35 and -10 regions, the last base of the Las Box was cropped and the -35 region was modified to overlap with the first two bases of the Las Box.
mutable provenance: The LasR binding site is from the region upstream of the LasI gene in Pseudomonas aeruginosa. The rest of the promoter is the same as BBa_K091156.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35 Region
sequence annotation: LuxR+AI-1
sequence annotation: -10 Region
sequence annotation: LasR + PAI-1
sequence: https://synbiohub.org/public/igem/BBa_K091157_sequence/1

-35 Region
type: SequenceAnnotation
persistent identity: annotation1968423
display id: annotation1968423
version: 1
top level: BBa_K091157
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K091157/annotation1968423/range1968423/1

https://synbiohub.org/public/igem/BBa_K091157/annotation1968423/range1968423/1
type: Range
persistent identity: range1968423
display id: range1968423
version: 1
direction: direction/forward
top level: BBa_K091157
start: 20
end: 25
orientation: inline

LuxR+AI-1
type: SequenceAnnotation
persistent identity: annotation1968426
display id: annotation1968426
version: 1
top level: BBa_K091157
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K091157/annotation1968426/range1968426/1

https://synbiohub.org/public/igem/BBa_K091157/annotation1968426/range1968426/1
type: Range
persistent identity: range1968426
display id: range1968426
version: 1
direction: direction/forward
top level: BBa_K091157
start: 1
end: 20
orientation: inline

-10 Region
type: SequenceAnnotation
persistent identity: annotation1968424
display id: annotation1968424
version: 1
top level: BBa_K091157
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K091157/annotation1968424/range1968424/1

https://synbiohub.org/public/igem/BBa_K091157/annotation1968424/range1968424/1
type: Range
persistent identity: range1968424
display id: range1968424
version: 1
direction: direction/forward
top level: BBa_K091157
start: 42
end: 47
orientation: inline

LasR + PAI-1
type: SequenceAnnotation
persistent identity: annotation1968425
display id: annotation1968425
version: 1
top level: BBa_K091157
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K091157/annotation1968425/range1968425/1

https://synbiohub.org/public/igem/BBa_K091157/annotation1968425/range1968425/1
type: Range
persistent identity: range1968425
display id: range1968425
version: 1
direction: direction/forward
top level: BBa_K091157
start: 24
end: 42
orientation: inline

BBa_I751501
type: ComponentDefinition
persistent identity: BBa I751501
display id: BBa_I751501
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: plux-cI hybrid promoter
creator: Kenichiro Iwasaki
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:08:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _151_
m user id: 0
owner id: 2188
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: sense TWO INPUTS, activation by AHL and repression by LacI.
mutable notes: Based on the AHL regulated BioBrick part R0062 and the previous work on LacI regulated promoter (ref. 1), we designed a new promoter regulated by AHL and LacI. Since this promoter is a kind of a "HYBRID" of the two, we call it HYBRID PROMOTER. Compared with existing BioBrick hybrid promoter R0065, this promoter is different in that its sequence is much shorter with lux box and -35 region overlapping, and that it is repressed by LacI, not cI. Also, the output is clearly detected as shown in Fig. 3.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: plux-cI hybrid promoter
sequence annotation: luxR binding
sequence annotation: OR2
sequence annotation: OR1
sequence annotation: -10
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_I751501_sequence/1

plux-cI hybrid promoter
type: SequenceAnnotation
persistent identity: annotation1955478
display id: annotation1955478
version: 1
top level: BBa_I751501
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I751501/annotation1955478/range1955478/1

https://synbiohub.org/public/igem/BBa_I751501/annotation1955478/range1955478/1
type: Range
persistent identity: range1955478
display id: range1955478
version: 1
direction: direction/forward
top level: BBa_I751501
start: 19
end: 66
orientation: inline

luxR binding
type: SequenceAnnotation
persistent identity: annotation1955482
display id: annotation1955482
version: 1
top level: BBa_I751501
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I751501/annotation1955482/range1955482/1

https://synbiohub.org/public/igem/BBa_I751501/annotation1955482/range1955482/1
type: Range
persistent identity: range1955482
display id: range1955482
version: 1
direction: direction/forward
top level: BBa_I751501
start: 1
end: 18
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation1955481
display id: annotation1955481
version: 1
top level: BBa_I751501
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I751501/annotation1955481/range1955481/1

https://synbiohub.org/public/igem/BBa_I751501/annotation1955481/range1955481/1
type: Range
persistent identity: range1955481
display id: range1955481
version: 1
direction: direction/forward
top level: BBa_I751501
start: 26
end: 41
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation1955477
display id: annotation1955477
version: 1
top level: BBa_I751501
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I751501/annotation1955477/range1955477/1

https://synbiohub.org/public/igem/BBa_I751501/annotation1955477/range1955477/1
type: Range
persistent identity: range1955477
display id: range1955477
version: 1
direction: direction/forward
top level: BBa_I751501
start: 48
end: 66
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1955480
display id: annotation1955480
version: 1
top level: BBa_I751501
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I751501/annotation1955480/range1955480/1

https://synbiohub.org/public/igem/BBa_I751501/annotation1955480/range1955480/1
type: Range
persistent identity: range1955480
display id: range1955480
version: 1
direction: direction/forward
top level: BBa_I751501
start: 42
end: 47
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1955479
display id: annotation1955479
version: 1
top level: BBa_I751501
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I751501/annotation1955479/range1955479/1

https://synbiohub.org/public/igem/BBa_I751501/annotation1955479/range1955479/1
type: Range
persistent identity: range1955479
display id: range1955479
version: 1
direction: direction/forward
top level: BBa_I751501
start: 19
end: 25
orientation: inline

BBa_K658008
type: ComponentDefinition
persistent identity: BBa K658008
display id: BBa_K658008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: position 3&5 mutated promoter lux pR-3/5 (luxR & HSL regulated)
creator: Yidan Hu
created: 2011-09-30T11:00:00Z
modified: 2015-05-08T01:13:02Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _835_
m user id: 0
owner id: 10207
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The position 3&5 mutated promoter lux pR-3/5 is a product of site-directed mutagenesis of promoter lux pR (BBa_R0062). The promoter lux pR is based on the Vibrio fischeri quorum sensing gene promoters. The position 5 mutated promoter lux pR-3/5 gives a lower expression of downstream genes in an autoinduction system. In another word, the lux pR-3/5 has a lower efficiency than the lux pR(BBa_R0062). Similar to promoter lux pR, the expression is up-regulated by the action of LuxR protein combined with the signal molecule HSL.
mutable notes: We have generated two point mutants by site-directed mutagenesis from the sequence of lux pR(BBa_R0062). In these mutants, the G residue of position 5 was changed to a C and the C residue of position 3 was changed to a T respectively.
mutable provenance: The promoter lux pR (BBa_R0062).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LuxR/HSL
sequence annotation: K658008
sequence annotation: start
sequence annotation: -10
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_K658008_sequence/1

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2148176
display id: annotation2148176
version: 1
top level: BBa_K658008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K658008/annotation2148176/range2148176/1

https://synbiohub.org/public/igem/BBa_K658008/annotation2148176/range2148176/1
type: Range
persistent identity: range2148176
display id: range2148176
version: 1
direction: direction/forward
top level: BBa_K658008
start: 1
end: 20
orientation: inline

K658008
type: SequenceAnnotation
persistent identity: annotation2148175
display id: annotation2148175
version: 1
top level: BBa_K658008
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K658008/annotation2148175/range2148175/1

https://synbiohub.org/public/igem/BBa_K658008/annotation2148175/range2148175/1
type: Range
persistent identity: range2148175
display id: range2148175
version: 1
direction: direction/forward
top level: BBa_K658008
start: 1
end: 55
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2148172
display id: annotation2148172
version: 1
top level: BBa_K658008
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K658008/annotation2148172/range2148172/1

https://synbiohub.org/public/igem/BBa_K658008/annotation2148172/range2148172/1
type: Range
persistent identity: range2148172
display id: range2148172
version: 1
direction: direction/forward
top level: BBa_K658008
start: 53
end: 53
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2148174
display id: annotation2148174
version: 1
top level: BBa_K658008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K658008/annotation2148174/range2148174/1

https://synbiohub.org/public/igem/BBa_K658008/annotation2148174/range2148174/1
type: Range
persistent identity: range2148174
display id: range2148174
version: 1
direction: direction/forward
top level: BBa_K658008
start: 42
end: 47
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2148173
display id: annotation2148173
version: 1
top level: BBa_K658008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K658008/annotation2148173/range2148173/1

https://synbiohub.org/public/igem/BBa_K658008/annotation2148173/range2148173/1
type: Range
persistent identity: range2148173
display id: range2148173
version: 1
direction: direction/forward
top level: BBa_K658008
start: 20
end: 25
orientation: inline

BBa_I739105
type: ComponentDefinition
persistent identity: BBa I739105
display id: BBa_I739105
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Double Promoter (LuxR/HSL, positive / cI, negative)
creator: Stefan Luzi
created: 2007-10-14T11:00:00Z
modified: 2015-08-31T04:08:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _124_
m user id: 0
owner id: 2183
owning group id: 62
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: asd
mutable notes: asdf
mutable provenance: Synthetic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR2
sequence annotation: Scar
sequence annotation: R0051 (2x OR2)
sequence annotation: R0062
sequence: https://synbiohub.org/public/igem/BBa_I739105_sequence/1

OR2
type: SequenceAnnotation
persistent identity: annotation1947917
display id: annotation1947917
version: 1
top level: BBa_I739105
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I739105/annotation1947917/range1947917/1

https://synbiohub.org/public/igem/BBa_I739105/annotation1947917/range1947917/1
type: Range
persistent identity: range1947917
display id: range1947917
version: 1
direction: direction/forward
top level: BBa_I739105
start: 82
end: 99
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation1947916
display id: annotation1947916
version: 1
top level: BBa_I739105
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I739105/annotation1947916/range1947916/1

https://synbiohub.org/public/igem/BBa_I739105/annotation1947916/range1947916/1
type: Range
persistent identity: range1947916
display id: range1947916
version: 1
direction: direction/forward
top level: BBa_I739105
start: 64
end: 81
orientation: inline

Scar
type: SequenceAnnotation
persistent identity: annotation1947915
display id: annotation1947915
version: 1
top level: BBa_I739105
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I739105/annotation1947915/range1947915/1

https://synbiohub.org/public/igem/BBa_I739105/annotation1947915/range1947915/1
type: Range
persistent identity: range1947915
display id: range1947915
version: 1
direction: direction/forward
top level: BBa_I739105
start: 56
end: 63
orientation: inline

R0051 (2x OR2)
type: SequenceAnnotation
persistent identity: annotation1947918
display id: annotation1947918
version: 1
top level: BBa_I739105
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I739105/annotation1947918/range1947918/1

https://synbiohub.org/public/igem/BBa_I739105/annotation1947918/range1947918/1
type: Range
persistent identity: range1947918
display id: range1947918
version: 1
direction: direction/forward
top level: BBa_I739105
start: 64
end: 99
orientation: inline

R0062
type: SequenceAnnotation
persistent identity: annotation1947914
display id: annotation1947914
version: 1
top level: BBa_I739105
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I739105/annotation1947914/range1947914/1

https://synbiohub.org/public/igem/BBa_I739105/annotation1947914/range1947914/1
type: Range
persistent identity: range1947914
display id: range1947914
version: 1
direction: direction/forward
top level: BBa_I739105
start: 1
end: 55
orientation: inline

BBa_K266007
type: ComponentDefinition
persistent identity: BBa K266007
display id: BBa_K266007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Complex QS -> LuxI & LasI circuit
creator: UNAM FCIENCIAS-CINVESTAV IRA
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _363_
m user id: 0
owner id: 1955
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Complex Quorum sensing circuit that receives the signal of PAI+LasR and AI+LuxR to control the production of LuxI and LasI enzymes.
mutable notes: None
mutable provenance: The biobricks BBa_K266006 and BBa_K266000 where used to build this biobrick
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_K266000
component: BBa_K266006
sequence annotation: BBa_K266000
sequence annotation: BBa_K266006
sequence: https://synbiohub.org/public/igem/BBa_K266007_sequence/1

BBa_K266000
type: Component
persistent identity: component2297452
display id: component2297452
version: 1
top level: BBa_K266007
access: public
definition: https://synbiohub.org/public/igem/BBa_K266000/1

BBa_K266006
type: Component
persistent identity: component2297433
display id: component2297433
version: 1
top level: BBa_K266007
access: public
definition: BBa_K266006

BBa_K266000
type: SequenceAnnotation
persistent identity: annotation2297452
display id: annotation2297452
version: 1
top level: BBa_K266007
location: https://synbiohub.org/public/igem/BBa_K266007/annotation2297452/range2297452/1
component: BBa_K266000

https://synbiohub.org/public/igem/BBa_K266007/annotation2297452/range2297452/1
type: Range
persistent identity: range2297452
display id: range2297452
version: 1
direction: direction/reverse
top level: BBa_K266007
start: 1714
end: 2676
orientation: inline

BBa_K266006
type: SequenceAnnotation
persistent identity: annotation2297433
display id: annotation2297433
version: 1
top level: BBa_K266007
location: https://synbiohub.org/public/igem/BBa_K266007/annotation2297433/range2297433/1
component: BBa_K266006

https://synbiohub.org/public/igem/BBa_K266007/annotation2297433/range2297433/1
type: Range
persistent identity: range2297433
display id: range2297433
version: 1
direction: direction/reverse
top level: BBa_K266007
start: 1
end: 1705
orientation: inline

BBa_K1520515
type: ComponentDefinition
persistent identity: BBa K1520515
display id: BBa_K1520515
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MC7-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-OMPA-golB-rbs-luxI-Ter.
creator: yue wu
created: 2014-09-22T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: MC7-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-OMPA-golB-rbs-luxI-Ter. It is a test of our positive feedback circuit for microcystin.
mutable notes: None
mutable provenance: MC7, luxI, Pcons2, luxR, Prlux, OMPA-golB, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520029
component: BBa_K1520504
component: BBa_K1520507
component: BBa_K1520003
component: BBa_K1520001
component: BBa_R0062
sequence annotation: BBa_K1520504
sequence annotation: BBa_K1520003
sequence annotation: BBa_K1520029
sequence annotation: BBa_K1520507
sequence annotation: BBa_R0062
sequence annotation: BBa_K1520001
sequence: https://synbiohub.org/public/igem/BBa_K1520515_sequence/1

BBa_K1520029
type: Component
persistent identity: component2385691
display id: component2385691
version: 1
top level: BBa_K1520515
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_K1520504
type: Component
persistent identity: component2385668
display id: component2385668
version: 1
top level: BBa_K1520515
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520504/1

BBa_K1520507
type: Component
persistent identity: component2385687
display id: component2385687
version: 1
top level: BBa_K1520515
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520507/1

BBa_K1520003
type: Component
persistent identity: component2385675
display id: component2385675
version: 1
top level: BBa_K1520515
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520003/1

BBa_K1520029
type: Component
persistent identity: component2385669
display id: component2385669
version: 1
top level: BBa_K1520515
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_K1520504
type: Component
persistent identity: component2385690
display id: component2385690
version: 1
top level: BBa_K1520515
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520504/1

BBa_K1520001
type: Component
persistent identity: component2385665
display id: component2385665
version: 1
top level: BBa_K1520515
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520001/1

BBa_R0062
type: Component
persistent identity: component2385677
display id: component2385677
version: 1
top level: BBa_K1520515
access: public
definition: lux pR

BBa_K1520504
type: SequenceAnnotation
persistent identity: annotation2385668
display id: annotation2385668
version: 1
top level: BBa_K1520515
location: https://synbiohub.org/public/igem/BBa_K1520515/annotation2385668/range2385668/1
component: BBa_K1520504

https://synbiohub.org/public/igem/BBa_K1520515/annotation2385668/range2385668/1
type: Range
persistent identity: range2385668
display id: range2385668
version: 1
direction: direction/reverse
top level: BBa_K1520515
start: 142
end: 802
orientation: inline

BBa_K1520003
type: SequenceAnnotation
persistent identity: annotation2385675
display id: annotation2385675
version: 1
top level: BBa_K1520515
location: https://synbiohub.org/public/igem/BBa_K1520515/annotation2385675/range2385675/1
component: BBa_K1520003

https://synbiohub.org/public/igem/BBa_K1520515/annotation2385675/range2385675/1
type: Range
persistent identity: range2385675
display id: range2385675
version: 1
direction: direction/reverse
top level: BBa_K1520515
start: 948
end: 1926
orientation: inline

BBa_K1520504
type: SequenceAnnotation
persistent identity: annotation2385690
display id: annotation2385690
version: 1
top level: BBa_K1520515
location: https://synbiohub.org/public/igem/BBa_K1520515/annotation2385690/range2385690/1
component: BBa_K1520504

https://synbiohub.org/public/igem/BBa_K1520515/annotation2385690/range2385690/1
type: Range
persistent identity: range2385690
display id: range2385690
version: 1
direction: direction/reverse
top level: BBa_K1520515
start: 2814
end: 3474
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2385669
display id: annotation2385669
version: 1
top level: BBa_K1520515
location: https://synbiohub.org/public/igem/BBa_K1520515/annotation2385669/range2385669/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520515/annotation2385669/range2385669/1
type: Range
persistent identity: range2385669
display id: range2385669
version: 1
direction: direction/reverse
top level: BBa_K1520515
start: 811
end: 939
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2385691
display id: annotation2385691
version: 1
top level: BBa_K1520515
location: https://synbiohub.org/public/igem/BBa_K1520515/annotation2385691/range2385691/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520515/annotation2385691/range2385691/1
type: Range
persistent identity: range2385691
display id: range2385691
version: 1
direction: direction/reverse
top level: BBa_K1520515
start: 3483
end: 3611
orientation: inline

BBa_K1520507
type: SequenceAnnotation
persistent identity: annotation2385687
display id: annotation2385687
version: 1
top level: BBa_K1520515
location: https://synbiohub.org/public/igem/BBa_K1520515/annotation2385687/range2385687/1
component: BBa_K1520507

https://synbiohub.org/public/igem/BBa_K1520515/annotation2385687/range2385687/1
type: Range
persistent identity: range2385687
display id: range2385687
version: 1
direction: direction/reverse
top level: BBa_K1520515
start: 1996
end: 2805
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2385677
display id: annotation2385677
version: 1
top level: BBa_K1520515
location: https://synbiohub.org/public/igem/BBa_K1520515/annotation2385677/range2385677/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K1520515/annotation2385677/range2385677/1
type: Range
persistent identity: range2385677
display id: range2385677
version: 1
direction: direction/forward
top level: BBa_K1520515
start: 1935
end: 1989
orientation: inline

BBa_K1520001
type: SequenceAnnotation
persistent identity: annotation2385665
display id: annotation2385665
version: 1
top level: BBa_K1520515
location: https://synbiohub.org/public/igem/BBa_K1520515/annotation2385665/range2385665/1
component: BBa_K1520001

https://synbiohub.org/public/igem/BBa_K1520515/annotation2385665/range2385665/1
type: Range
persistent identity: range2385665
display id: range2385665
version: 1
direction: direction/reverse
top level: BBa_K1520515
start: 1
end: 133
orientation: inline

BBa_K266006
type: ComponentDefinition
persistent identity: BBa K266006
display id: BBa_K266006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PAI+LasR -> LasI+GFP & AI+LuxR --| LasI+GFP
creator: UNAM FCIENCIAS-CINVESTAV IRA
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _363_
m user id: 0
owner id: 1955
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: BBa_K091146 promoter (PAI+LasR inducible & AI+LuxR repressible) controls the policistronic expresion of LasI enzyme and GFP, double terminator
mutable notes: None.
mutable provenance: The biobricks BBa_K266005 and BBa_E0840 where used to build this biobrick.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_K266005
component: BBa_E0840
sequence annotation: BBa_K266005
sequence annotation: BBa_E0840
sequence: https://synbiohub.org/public/igem/BBa_K266006_sequence/1

BBa_K266005
type: Component
persistent identity: component2267607
display id: component2267607
version: 1
top level: BBa_K266006
access: public
definition: BBa_K266005

BBa_E0840
type: Component
persistent identity: component2267618
display id: component2267618
version: 1
top level: BBa_K266006
access: public
definition: https://synbiohub.org/public/igem/BBa_E0840/1

BBa_K266005
type: SequenceAnnotation
persistent identity: annotation2267607
display id: annotation2267607
version: 1
top level: BBa_K266006
location: https://synbiohub.org/public/igem/BBa_K266006/annotation2267607/range2267607/1
component: BBa_K266005

https://synbiohub.org/public/igem/BBa_K266006/annotation2267607/range2267607/1
type: Range
persistent identity: range2267607
display id: range2267607
version: 1
direction: direction/reverse
top level: BBa_K266006
start: 1
end: 819
orientation: inline

BBa_E0840
type: SequenceAnnotation
persistent identity: annotation2267618
display id: annotation2267618
version: 1
top level: BBa_K266006
location: https://synbiohub.org/public/igem/BBa_K266006/annotation2267618/range2267618/1
component: BBa_E0840

https://synbiohub.org/public/igem/BBa_K266006/annotation2267618/range2267618/1
type: Range
persistent identity: range2267618
display id: range2267618
version: 1
direction: direction/reverse
top level: BBa_K266006
start: 828
end: 1705
orientation: inline

lux pR
type: ComponentDefinition
persistent identity: BBa R0062
display id: BBa_R0062
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (luxR & HSL regulated -- lux pR)
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Promoter activated by LuxR in concert with HSL The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
mutable notes: This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <img src="<bb_file>Image1.gif</bb_file>" width="614" height="362">
mutable provenance: V. fischeri
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: start
sequence annotation: -35
sequence annotation: -10
sequence annotation: BBa_R0062
sequence annotation: LuxR/HSL
sequence: https://synbiohub.org/public/igem/BBa_R0062_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2048
display id: annotation2048
version: 1
top level: lux pR
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1

https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1
type: Range
persistent identity: range2048
display id: range2048
version: 1
direction: direction/forward
top level: lux pR
start: 53
end: 53
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2046
display id: annotation2046
version: 1
top level: lux pR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1

https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1
type: Range
persistent identity: range2046
display id: range2046
version: 1
direction: direction/forward
top level: lux pR
start: 20
end: 25
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2047
display id: annotation2047
version: 1
top level: lux pR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1

https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1
type: Range
persistent identity: range2047
display id: range2047
version: 1
direction: direction/forward
top level: lux pR
start: 42
end: 47
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation7070
display id: annotation7070
version: 1
top level: lux pR
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1

https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1
type: Range
persistent identity: range7070
display id: range7070
version: 1
direction: direction/forward
top level: lux pR
start: 1
end: 55
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2045
display id: annotation2045
version: 1
top level: lux pR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1

https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1
type: Range
persistent identity: range2045
display id: range2045
version: 1
direction: direction/forward
top level: lux pR
start: 1
end: 20
orientation: inline

lux pR-5
type: ComponentDefinition
persistent identity: BBa K658007
display id: BBa_K658007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: position 5 mutated promoter lux pR-5 (luxR & HSL regulated)
creator: Yidan Hu
created: 2011-09-30T11:00:00Z
modified: 2015-05-08T01:13:02Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _835_
m user id: 0
owner id: 10207
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The position 5 mutated promoter lux pR-5 is a product of site-directed mutagenesis of promoter lux pR (BBa_R0062). The promoter lux pR is based on the Vibrio fischeri quorum sensing gene promoters. The position 5 mutated promoter lux pR-5 gives a higher expression of downstream genes in an autoinduction system. In another word, the lux pR-5 has a higher efficiency than the lux pR. Similar to promoter lux pR, the expression is up-regulated by the action of LuxR protein combined with the signal molecule HSL.
mutable notes: By PCR method the G residue of position 5 was changed to a C based on the sequence of the promoter lux pR(BBa_R0062).
mutable provenance: The promoter lux pR (BBa_R0062).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: start
sequence annotation: LuxR/HSL
sequence annotation: K658007
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_K658007_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation2148170
display id: annotation2148170
version: 1
top level: lux pR-5
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K658007/annotation2148170/range2148170/1

https://synbiohub.org/public/igem/BBa_K658007/annotation2148170/range2148170/1
type: Range
persistent identity: range2148170
display id: range2148170
version: 1
direction: direction/forward
top level: lux pR-5
start: 20
end: 25
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2148167
display id: annotation2148167
version: 1
top level: lux pR-5
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K658007/annotation2148167/range2148167/1

https://synbiohub.org/public/igem/BBa_K658007/annotation2148167/range2148167/1
type: Range
persistent identity: range2148167
display id: range2148167
version: 1
direction: direction/forward
top level: lux pR-5
start: 53
end: 53
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2148169
display id: annotation2148169
version: 1
top level: lux pR-5
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K658007/annotation2148169/range2148169/1

https://synbiohub.org/public/igem/BBa_K658007/annotation2148169/range2148169/1
type: Range
persistent identity: range2148169
display id: range2148169
version: 1
direction: direction/forward
top level: lux pR-5
start: 1
end: 20
orientation: inline

K658007
type: SequenceAnnotation
persistent identity: annotation2148168
display id: annotation2148168
version: 1
top level: lux pR-5
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K658007/annotation2148168/range2148168/1

https://synbiohub.org/public/igem/BBa_K658007/annotation2148168/range2148168/1
type: Range
persistent identity: range2148168
display id: range2148168
version: 1
direction: direction/forward
top level: lux pR-5
start: 1
end: 55
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2148171
display id: annotation2148171
version: 1
top level: lux pR-5
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K658007/annotation2148171/range2148171/1

https://synbiohub.org/public/igem/BBa_K658007/annotation2148171/range2148171/1
type: Range
persistent identity: range2148171
display id: range2148171
version: 1
direction: direction/forward
top level: lux pR-5
start: 42
end: 47
orientation: inline

BBa_K145150
type: ComponentDefinition
persistent identity: BBa K145150
display id: BBa_K145150
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Hybrid promoter: HSL-LuxR activated, P22 C2 repressed
creator: Jonas Demeulemeester
created: 2008-08-05T11:00:00Z
modified: 2015-05-08T01:10:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _257_
m user id: 0
owner id: 2970
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Hybrid promoter consisting of the Lux box which overlaps partly with -35 box. Binding sites for P22 C2 OR2 and OR1 are located between the -35 and -10 boxes and downstream of them respectively.
mutable notes: -35 box is partly composite. -10 is taken from the P22 phage PR regulatory region
mutable provenance: Synthetic
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: Lux-box
sequence annotation: OR2
sequence annotation: OR1
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_K145150_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1971585
display id: annotation1971585
version: 1
top level: BBa_K145150
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K145150/annotation1971585/range1971585/1

https://synbiohub.org/public/igem/BBa_K145150/annotation1971585/range1971585/1
type: Range
persistent identity: range1971585
display id: range1971585
version: 1
direction: direction/forward
top level: BBa_K145150
start: 42
end: 47
orientation: inline

Lux-box
type: SequenceAnnotation
persistent identity: annotation1971582
display id: annotation1971582
version: 1
top level: BBa_K145150
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K145150/annotation1971582/range1971582/1

https://synbiohub.org/public/igem/BBa_K145150/annotation1971582/range1971582/1
type: Range
persistent identity: range1971582
display id: range1971582
version: 1
direction: direction/forward
top level: BBa_K145150
start: 1
end: 20
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation1971584
display id: annotation1971584
version: 1
top level: BBa_K145150
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K145150/annotation1971584/range1971584/1

https://synbiohub.org/public/igem/BBa_K145150/annotation1971584/range1971584/1
type: Range
persistent identity: range1971584
display id: range1971584
version: 1
direction: direction/forward
top level: BBa_K145150
start: 23
end: 40
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation1971586
display id: annotation1971586
version: 1
top level: BBa_K145150
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K145150/annotation1971586/range1971586/1

https://synbiohub.org/public/igem/BBa_K145150/annotation1971586/range1971586/1
type: Range
persistent identity: range1971586
display id: range1971586
version: 1
direction: direction/forward
top level: BBa_K145150
start: 46
end: 63
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1971583
display id: annotation1971583
version: 1
top level: BBa_K145150
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K145150/annotation1971583/range1971583/1

https://synbiohub.org/public/igem/BBa_K145150/annotation1971583/range1971583/1
type: Range
persistent identity: range1971583
display id: range1971583
version: 1
direction: direction/forward
top level: BBa_K145150
start: 20
end: 25
orientation: inline

BBa_K1520010
type: ComponentDefinition
persistent identity: BBa K1520010
display id: BBa_K1520010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Prlux-rbs-rfp-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Prlux-rbs-rfp-Ter. It produces rfp when there is enough luxR and AHL.
mutable notes: None
mutable provenance: Prlux, rfp, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1520500
component: BBa_R0062
component: BBa_K1520029
sequence annotation: BBa_K1520029
sequence annotation: BBa_R0062
sequence annotation: BBa_K1520500
sequence: https://synbiohub.org/public/igem/BBa_K1520010_sequence/1

BBa_K1520500
type: Component
persistent identity: component2384954
display id: component2384954
version: 1
top level: BBa_K1520010
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520500/1

BBa_R0062
type: Component
persistent identity: component2384949
display id: component2384949
version: 1
top level: BBa_K1520010
access: public
definition: lux pR

BBa_K1520029
type: Component
persistent identity: component2384955
display id: component2384955
version: 1
top level: BBa_K1520010
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2384955
display id: annotation2384955
version: 1
top level: BBa_K1520010
location: https://synbiohub.org/public/igem/BBa_K1520010/annotation2384955/range2384955/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520010/annotation2384955/range2384955/1
type: Range
persistent identity: range2384955
display id: range2384955
version: 1
direction: direction/reverse
top level: BBa_K1520010
start: 804
end: 932
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2384949
display id: annotation2384949
version: 1
top level: BBa_K1520010
location: https://synbiohub.org/public/igem/BBa_K1520010/annotation2384949/range2384949/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K1520010/annotation2384949/range2384949/1
type: Range
persistent identity: range2384949
display id: range2384949
version: 1
direction: direction/forward
top level: BBa_K1520010
start: 1
end: 55
orientation: inline

BBa_K1520500
type: SequenceAnnotation
persistent identity: annotation2384954
display id: annotation2384954
version: 1
top level: BBa_K1520010
location: https://synbiohub.org/public/igem/BBa_K1520010/annotation2384954/range2384954/1
component: BBa_K1520500

https://synbiohub.org/public/igem/BBa_K1520010/annotation2384954/range2384954/1
type: Range
persistent identity: range2384954
display id: range2384954
version: 1
direction: direction/reverse
top level: BBa_K1520010
start: 64
end: 795
orientation: inline

BBa_K091156
type: ComponentDefinition
persistent identity: BBa K091156
display id: BBa_K091156
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLux
creator: Erin Feeney
created: 2008-07-24T11:00:00Z
modified: 2015-05-08T01:08:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _191_
m user id: 0
owner id: 3067
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The pLux' promoter is a modified version of BBa_R0062 containing the -35 region TTTAAT in place of TTTACG. Transcriptional activation is expected to occur in the presence of LuxR and AI-1, just as with R0062. The modified -35 region was designed to serve as a control for the evaluation of BBa_K091156, the pLux+ Las- promoter.
mutable notes: This promoter is identical to pLux+ Las- with the exception of the Las box's absence
mutable provenance: This part was adapted from BBa_R0062.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35 Region
sequence annotation: LuxR
sequence annotation: -10 Region
sequence: https://synbiohub.org/public/igem/BBa_K091156_sequence/1

-35 Region
type: SequenceAnnotation
persistent identity: annotation1968420
display id: annotation1968420
version: 1
top level: BBa_K091156
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K091156/annotation1968420/range1968420/1

https://synbiohub.org/public/igem/BBa_K091156/annotation1968420/range1968420/1
type: Range
persistent identity: range1968420
display id: range1968420
version: 1
direction: direction/forward
top level: BBa_K091156
start: 20
end: 25
orientation: inline

LuxR
type: SequenceAnnotation
persistent identity: annotation1968422
display id: annotation1968422
version: 1
top level: BBa_K091156
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K091156/annotation1968422/range1968422/1

https://synbiohub.org/public/igem/BBa_K091156/annotation1968422/range1968422/1
type: Range
persistent identity: range1968422
display id: range1968422
version: 1
direction: direction/forward
top level: BBa_K091156
start: 1
end: 20
orientation: inline

-10 Region
type: SequenceAnnotation
persistent identity: annotation1968421
display id: annotation1968421
version: 1
top level: BBa_K091156
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K091156/annotation1968421/range1968421/1

https://synbiohub.org/public/igem/BBa_K091156/annotation1968421/range1968421/1
type: Range
persistent identity: range1968421
display id: range1968421
version: 1
direction: direction/forward
top level: BBa_K091156
start: 42
end: 47
orientation: inline

Lux
type: ComponentDefinition
persistent identity: BBa I1051
display id: BBa_I1051
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lux cassette right promoter
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:30Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.In thisversion, a binding site for cI dimer has been added: binding to this site dominantly represses transcription.
mutable provenance: V. fishceri and phage lambda (courtesy of Ron Weiss)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: LuxR/HSL
sequence annotation: -35
sequence annotation: BBa_I1051
sequence annotation: cI (OR1)
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_I1051_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1938
display id: annotation1938
version: 1
top level: Lux
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I1051/annotation1938/range1938/1

https://synbiohub.org/public/igem/BBa_I1051/annotation1938/range1938/1
type: Range
persistent identity: range1938
display id: range1938
version: 1
direction: direction/forward
top level: Lux
start: 42
end: 47
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation1936
display id: annotation1936
version: 1
top level: Lux
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I1051/annotation1936/range1936/1

https://synbiohub.org/public/igem/BBa_I1051/annotation1936/range1936/1
type: Range
persistent identity: range1936
display id: range1936
version: 1
direction: direction/forward
top level: Lux
start: 1
end: 20
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1937
display id: annotation1937
version: 1
top level: Lux
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I1051/annotation1937/range1937/1

https://synbiohub.org/public/igem/BBa_I1051/annotation1937/range1937/1
type: Range
persistent identity: range1937
display id: range1937
version: 1
direction: direction/forward
top level: Lux
start: 20
end: 25
orientation: inline

BBa_I1051
type: SequenceAnnotation
persistent identity: annotation7059
display id: annotation7059
version: 1
top level: Lux
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I1051/annotation7059/range7059/1

https://synbiohub.org/public/igem/BBa_I1051/annotation7059/range7059/1
type: Range
persistent identity: range7059
display id: range7059
version: 1
direction: direction/forward
top level: Lux
start: 1
end: 68
orientation: inline

cI (OR1)
type: SequenceAnnotation
persistent identity: annotation1939
display id: annotation1939
version: 1
top level: Lux
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I1051/annotation1939/range1939/1

https://synbiohub.org/public/igem/BBa_I1051/annotation1939/range1939/1
type: Range
persistent identity: range1939
display id: range1939
version: 1
direction: direction/forward
top level: Lux
start: 52
end: 68
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1940
display id: annotation1940
version: 1
top level: Lux
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I1051/annotation1940/range1940/1

https://synbiohub.org/public/igem/BBa_I1051/annotation1940/range1940/1
type: Range
persistent identity: range1940
display id: range1940
version: 1
direction: direction/forward
top level: Lux
start: 53
end: 53
orientation: inline

luxR
type: ComponentDefinition
persistent identity: BBa R0061
display id: BBa_R0061
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (HSL-mediated luxR repressor)
creator: Srini Devadas, David Gray, Ronny Krashinsky, Debra Lin, and Chris Zheng Liu
created: 2004-01-28T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part involves the -10 binding site, the -35 binding site, and the twenty nucleotides between that constitute the lux box. With this part, LuxR functions as a acyl-homoserine lactone-dependent repressor. LuxR resonds to the HSL produced by LuxI, N-(3-oxohexanoyl)-HSL. The Lux box is positioned such that it partially overlaps the consensus -35 and -10 hexamers of an RNA polymerase binding site.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: -10
sequence annotation: Lux Box
sequence: https://synbiohub.org/public/igem/BBa_R0061_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation305863
display id: annotation305863
version: 1
top level: luxR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0061/annotation305863/range305863/1

https://synbiohub.org/public/igem/BBa_R0061/annotation305863/range305863/1
type: Range
persistent identity: range305863
display id: range305863
version: 1
direction: direction/forward
top level: luxR
start: 1
end: 6
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation305864
display id: annotation305864
version: 1
top level: luxR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0061/annotation305864/range305864/1

https://synbiohub.org/public/igem/BBa_R0061/annotation305864/range305864/1
type: Range
persistent identity: range305864
display id: range305864
version: 1
direction: direction/forward
top level: luxR
start: 25
end: 30
orientation: inline

Lux Box
type: SequenceAnnotation
persistent identity: annotation305877
display id: annotation305877
version: 1
top level: luxR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0061/annotation305877/range305877/1

https://synbiohub.org/public/igem/BBa_R0061/annotation305877/range305877/1
type: Range
persistent identity: range305877
display id: range305877
version: 1
direction: direction/forward
top level: luxR
start: 6
end: 25
orientation: inline

BBa_I751502
type: ComponentDefinition
persistent identity: BBa I751502
display id: BBa_I751502
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: plux-lac hybrid promoter
creator: Kenichiro Iwasaki
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:08:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _151_
m user id: 0
owner id: 2188
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: sense TWO INPUTS, activation by AHL and repression by LacI.
mutable notes: Compared with existing BioBrick hybrid promoter R0065, this promoter is different in that its sequence is much shorter with lux box and -35 region overlapping, and that it is repressed by LacI, not cI. Also, the output is clearly detected as shown in Fig. 3.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: O1
sequence annotation: plac
sequence annotation: -10
sequence annotation: luxR binding site
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_I751502_sequence/1

O1
type: SequenceAnnotation
persistent identity: annotation1955562
display id: annotation1955562
version: 1
top level: BBa_I751502
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I751502/annotation1955562/range1955562/1

https://synbiohub.org/public/igem/BBa_I751502/annotation1955562/range1955562/1
type: Range
persistent identity: range1955562
display id: range1955562
version: 1
direction: direction/forward
top level: BBa_I751502
start: 27
end: 41
orientation: inline

plac
type: SequenceAnnotation
persistent identity: annotation1955559
display id: annotation1955559
version: 1
top level: BBa_I751502
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I751502/annotation1955559/range1955559/1

https://synbiohub.org/public/igem/BBa_I751502/annotation1955559/range1955559/1
type: Range
persistent identity: range1955559
display id: range1955559
version: 1
direction: direction/forward
top level: BBa_I751502
start: 20
end: 74
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1955561
display id: annotation1955561
version: 1
top level: BBa_I751502
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I751502/annotation1955561/range1955561/1

https://synbiohub.org/public/igem/BBa_I751502/annotation1955561/range1955561/1
type: Range
persistent identity: range1955561
display id: range1955561
version: 1
direction: direction/forward
top level: BBa_I751502
start: 42
end: 48
orientation: inline

O1
type: SequenceAnnotation
persistent identity: annotation1955563
display id: annotation1955563
version: 1
top level: BBa_I751502
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I751502/annotation1955563/range1955563/1

https://synbiohub.org/public/igem/BBa_I751502/annotation1955563/range1955563/1
type: Range
persistent identity: range1955563
display id: range1955563
version: 1
direction: direction/forward
top level: BBa_I751502
start: 49
end: 74
orientation: inline

luxR binding site
type: SequenceAnnotation
persistent identity: annotation1955564
display id: annotation1955564
version: 1
top level: BBa_I751502
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I751502/annotation1955564/range1955564/1

https://synbiohub.org/public/igem/BBa_I751502/annotation1955564/range1955564/1
type: Range
persistent identity: range1955564
display id: range1955564
version: 1
direction: direction/forward
top level: BBa_I751502
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1955560
display id: annotation1955560
version: 1
top level: BBa_I751502
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I751502/annotation1955560/range1955560/1

https://synbiohub.org/public/igem/BBa_I751502/annotation1955560/range1955560/1
type: Range
persistent identity: range1955560
display id: range1955560
version: 1
direction: direction/forward
top level: BBa_I751502
start: 20
end: 26
orientation: inline

BBa_J64067
type: ComponentDefinition
persistent identity: BBa J64067
display id: BBa_J64067
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LuxR+3OC6HSL independent R0065
creator: Jeffrey J Tabor
created: 2007-12-12T12:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 88
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The -35 box of R0065 has been modified to promote constitutive (LuxR+3OC6HSL-independent) gene expression. An extended -10 sequence has also been added to increase absolute transcription. The promoter still contains the operators OR1 and OR2 such that it retains its sensitivity to CI.
mutable notes: see above
mutable provenance: modified from R0065
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR2
sequence annotation: extended -10
sequence annotation: -35
sequence annotation: OR1
sequence annotation: lux box
sequence annotation: +1
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_J64067_sequence/1

OR2
type: SequenceAnnotation
persistent identity: annotation1959023
display id: annotation1959023
version: 1
top level: BBa_J64067
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J64067/annotation1959023/range1959023/1

https://synbiohub.org/public/igem/BBa_J64067/annotation1959023/range1959023/1
type: Range
persistent identity: range1959023
display id: range1959023
version: 1
direction: direction/forward
top level: BBa_J64067
start: 58
end: 75
orientation: inline

extended -10
type: SequenceAnnotation
persistent identity: annotation1959019
display id: annotation1959019
version: 1
top level: BBa_J64067
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J64067/annotation1959019/range1959019/1

https://synbiohub.org/public/igem/BBa_J64067/annotation1959019/range1959019/1
type: Range
persistent identity: range1959019
display id: range1959019
version: 1
direction: direction/forward
top level: BBa_J64067
start: 43
end: 46
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1959018
display id: annotation1959018
version: 1
top level: BBa_J64067
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J64067/annotation1959018/range1959018/1

https://synbiohub.org/public/igem/BBa_J64067/annotation1959018/range1959018/1
type: Range
persistent identity: range1959018
display id: range1959018
version: 1
direction: direction/forward
top level: BBa_J64067
start: 25
end: 30
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation1959024
display id: annotation1959024
version: 1
top level: BBa_J64067
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64067/annotation1959024/range1959024/1

https://synbiohub.org/public/igem/BBa_J64067/annotation1959024/range1959024/1
type: Range
persistent identity: range1959024
display id: range1959024
version: 1
direction: direction/forward
top level: BBa_J64067
start: 82
end: 98
orientation: inline

lux box
type: SequenceAnnotation
persistent identity: annotation1959021
display id: annotation1959021
version: 1
top level: BBa_J64067
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J64067/annotation1959021/range1959021/1

https://synbiohub.org/public/igem/BBa_J64067/annotation1959021/range1959021/1
type: Range
persistent identity: range1959021
display id: range1959021
version: 1
direction: direction/forward
top level: BBa_J64067
start: 6
end: 26
orientation: inline

+1
type: SequenceAnnotation
persistent identity: annotation1959022
display id: annotation1959022
version: 1
top level: BBa_J64067
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64067/annotation1959022/range1959022/1

https://synbiohub.org/public/igem/BBa_J64067/annotation1959022/range1959022/1
type: Range
persistent identity: range1959022
display id: range1959022
version: 1
direction: direction/forward
top level: BBa_J64067
start: 59
end: 59
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1959020
display id: annotation1959020
version: 1
top level: BBa_J64067
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J64067/annotation1959020/range1959020/1

https://synbiohub.org/public/igem/BBa_J64067/annotation1959020/range1959020/1
type: Range
persistent identity: range1959020
display id: range1959020
version: 1
direction: direction/forward
top level: BBa_J64067
start: 48
end: 53
orientation: inline

lux pR
type: ComponentDefinition
persistent identity: BBa R1062
display id: BBa_R1062
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter, Standard (luxR and HSL regulated -- lux pR)
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr Drew Endy
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: [Note: This is the same part as R0062 except that the -10 and -35 sites and spacing have been changed to comply with BBa_S0001]. Promoter activated by LuxR in concert with HSL The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
mutable notes: This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <img src="<bb_file part=BBa_R0062>Image1.gif</bb_file>" width="614" height="362"> Modified to comply with BBa_S0001: TTGACA-17N-GATACT
mutable provenance: V. fischeri
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R1062
sequence annotation: LuxR/HSL
sequence annotation: start
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R1062_sequence/1

BBa_R1062
type: SequenceAnnotation
persistent identity: annotation7077
display id: annotation7077
version: 1
top level: lux pR
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R1062/annotation7077/range7077/1

https://synbiohub.org/public/igem/BBa_R1062/annotation7077/range7077/1
type: Range
persistent identity: range7077
display id: range7077
version: 1
direction: direction/forward
top level: lux pR
start: 1
end: 56
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2098
display id: annotation2098
version: 1
top level: lux pR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R1062/annotation2098/range2098/1

https://synbiohub.org/public/igem/BBa_R1062/annotation2098/range2098/1
type: Range
persistent identity: range2098
display id: range2098
version: 1
direction: direction/forward
top level: lux pR
start: 1
end: 20
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2100
display id: annotation2100
version: 1
top level: lux pR
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_R1062/annotation2100/range2100/1

https://synbiohub.org/public/igem/BBa_R1062/annotation2100/range2100/1
type: Range
persistent identity: range2100
display id: range2100
version: 1
direction: direction/forward
top level: lux pR
start: 54
end: 54
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2101
display id: annotation2101
version: 1
top level: lux pR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1062/annotation2101/range2101/1

https://synbiohub.org/public/igem/BBa_R1062/annotation2101/range2101/1
type: Range
persistent identity: range2101
display id: range2101
version: 1
direction: direction/forward
top level: lux pR
start: 20
end: 25
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2099
display id: annotation2099
version: 1
top level: lux pR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1062/annotation2099/range2099/1

https://synbiohub.org/public/igem/BBa_R1062/annotation2099/range2099/1
type: Range
persistent identity: range2099
display id: range2099
version: 1
direction: direction/forward
top level: lux pR
start: 42
end: 48
orientation: inline

BBa_K266005
type: ComponentDefinition
persistent identity: BBa K266005
display id: BBa_K266005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PAI+LasR -> LasI & AI+LuxR --| LasI
creator: UNAM FCIENCIAS-CINVESTAV IRA
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _363_
m user id: 0
owner id: 1955
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: BBa_K091146 promoter (PAI+LasR inducible & AI+LuxR repressible) controls the expresion of LasI enzyme, no terminator
mutable notes: None
mutable provenance: The biobricks BBa_K091146 and BBa_S03154 where used to build this biobrick
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_K091146
component: BBa_S03154
sequence annotation: BBa_K091146
sequence annotation: BBa_S03154
sequence: https://synbiohub.org/public/igem/BBa_K266005_sequence/1

BBa_K091146
type: Component
persistent identity: component2246677
display id: component2246677
version: 1
top level: BBa_K266005
access: public
definition: BBa_K091146

BBa_S03154
type: Component
persistent identity: component2246684
display id: component2246684
version: 1
top level: BBa_K266005
access: public
definition: https://synbiohub.org/public/igem/BBa_S03154/1

BBa_K091146
type: SequenceAnnotation
persistent identity: annotation2246677
display id: annotation2246677
version: 1
top level: BBa_K266005
location: https://synbiohub.org/public/igem/BBa_K266005/annotation2246677/range2246677/1
component: BBa_K091146

https://synbiohub.org/public/igem/BBa_K266005/annotation2246677/range2246677/1
type: Range
persistent identity: range2246677
display id: range2246677
version: 1
direction: direction/reverse
top level: BBa_K266005
start: 1
end: 126
orientation: inline

BBa_S03154
type: SequenceAnnotation
persistent identity: annotation2246684
display id: annotation2246684
version: 1
top level: BBa_K266005
location: https://synbiohub.org/public/igem/BBa_K266005/annotation2246684/range2246684/1
component: BBa_S03154

https://synbiohub.org/public/igem/BBa_K266005/annotation2246684/range2246684/1
type: Range
persistent identity: range2246684
display id: range2246684
version: 1
direction: direction/reverse
top level: BBa_K266005
start: 135
end: 819
orientation: inline

BBa_J102001
type: ComponentDefinition
persistent identity: BBa J102001
display id: BBa_J102001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Reverse Lux Promoter
creator: Michael Pettigrew
created: 2010-07-01T11:00:00Z
modified: 2015-08-31T04:08:24Z
discontinued: false
dominant: false
group u list: _580_
m user id: 0
owner id: 5944
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This promoter is the right promoter in the lux cassette of Vibrio Fischeri. There is weak constitutive expression of downstream genes. Expression of downstream genes can be up-regulated by the action of the LuxR activator protein in complex with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein can form a complex with two molecules of the AHL. This causes a conformational change in the protein, allowing it to bind to a site on the lux promoter and induce transcription.
mutable notes: None.
mutable provenance: This part was obtained by taking the reverse complement of R0062, ordering appropriate oligos, then annealing and extending them.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: promoter
sequence: https://synbiohub.org/public/igem/BBa_J102001_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation2072374
display id: annotation2072374
version: 1
top level: BBa_J102001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J102001/annotation2072374/range2072374/1

https://synbiohub.org/public/igem/BBa_J102001/annotation2072374/range2072374/1
type: Range
persistent identity: range2072374
display id: range2072374
version: 1
direction: direction/reverse
top level: BBa_J102001
start: 1
end: 55
orientation: inline

lux pR-3
type: ComponentDefinition
persistent identity: BBa K658006
display id: BBa_K658006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: position 3 mutated promoter lux pR-3 (luxR & HSL regulated)
creator: Yidan Hu
created: 2011-09-30T11:00:00Z
modified: 2015-05-08T01:13:02Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _835_
m user id: 0
owner id: 10207
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The position 3 mutated promoter lux pR-3 is a product of site-directed mutagenesis of promoter lux pR (BBa_R0062). The promoter lux pR is based on the Vibrio fischeri quorum sensing gene promoters. The position 3 mutated promoter lux pR-3 gives a higher expression of downstream genes in an autoinduction system. In another word, the lux pR-3 has a higher efficiency than the lux pR. Similar to promoter lux pR, the expression is up-regulated by the action of LuxR protein combined with the signal molecule HSL.
mutable notes: In the promoter lux pR's sequence, the C residue of position 3 was changed to a T using PCR method.
mutable provenance: The position 3 mutated promoter lux pR-3 is based on promoter lux pR (BBa_R0062).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: -10
sequence annotation: start
sequence annotation: LuxR/HSL
sequence annotation: K658006
sequence: https://synbiohub.org/public/igem/BBa_K658006_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation2148161
display id: annotation2148161
version: 1
top level: lux pR-3
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K658006/annotation2148161/range2148161/1

https://synbiohub.org/public/igem/BBa_K658006/annotation2148161/range2148161/1
type: Range
persistent identity: range2148161
display id: range2148161
version: 1
direction: direction/forward
top level: lux pR-3
start: 20
end: 25
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2148162
display id: annotation2148162
version: 1
top level: lux pR-3
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K658006/annotation2148162/range2148162/1

https://synbiohub.org/public/igem/BBa_K658006/annotation2148162/range2148162/1
type: Range
persistent identity: range2148162
display id: range2148162
version: 1
direction: direction/forward
top level: lux pR-3
start: 42
end: 47
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2148163
display id: annotation2148163
version: 1
top level: lux pR-3
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K658006/annotation2148163/range2148163/1

https://synbiohub.org/public/igem/BBa_K658006/annotation2148163/range2148163/1
type: Range
persistent identity: range2148163
display id: range2148163
version: 1
direction: direction/forward
top level: lux pR-3
start: 53
end: 53
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2148166
display id: annotation2148166
version: 1
top level: lux pR-3
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K658006/annotation2148166/range2148166/1

https://synbiohub.org/public/igem/BBa_K658006/annotation2148166/range2148166/1
type: Range
persistent identity: range2148166
display id: range2148166
version: 1
direction: direction/forward
top level: lux pR-3
start: 1
end: 20
orientation: inline

K658006
type: SequenceAnnotation
persistent identity: annotation2148164
display id: annotation2148164
version: 1
top level: lux pR-3
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K658006/annotation2148164/range2148164/1

https://synbiohub.org/public/igem/BBa_K658006/annotation2148164/range2148164/1
type: Range
persistent identity: range2148164
display id: range2148164
version: 1
direction: direction/forward
top level: lux pR-3
start: 1
end: 55
orientation: inline

BBa_K091107
type: ComponentDefinition
persistent identity: BBa K091107
display id: BBa_K091107
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLux/cI Hybrid Promoter
creator: Xiao Zhu
created: 2008-06-18T11:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3130
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This hybrid promoter is designed to respond to activation by LuxR when it is bound to AHL and to repression by the phage lambda cI repressor. The repression needs to win over activation.
mutable notes: This part is based on the idea of replacing the region of the Lux promoter downstream of the -35 with the OR2 cI half-operator, added LuxR-10 region. Based on what we have found up to now, promoters which are constructed just by inserting OR1/2 downstream of Lux are all very leaky.
mutable provenance: this part comes from LuxR promoter and cI lambda promoter.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Lux -10
sequence annotation: cI OR2
sequence annotation: Lux -35
sequence annotation: Lux Box
sequence: https://synbiohub.org/public/igem/BBa_K091107_sequence/1

Lux -10
type: SequenceAnnotation
persistent identity: annotation1963753
display id: annotation1963753
version: 1
top level: BBa_K091107
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K091107/annotation1963753/range1963753/1

https://synbiohub.org/public/igem/BBa_K091107/annotation1963753/range1963753/1
type: Range
persistent identity: range1963753
display id: range1963753
version: 1
direction: direction/forward
top level: BBa_K091107
start: 44
end: 49
orientation: inline

cI OR2
type: SequenceAnnotation
persistent identity: annotation1963752
display id: annotation1963752
version: 1
top level: BBa_K091107
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K091107/annotation1963752/range1963752/1

https://synbiohub.org/public/igem/BBa_K091107/annotation1963752/range1963752/1
type: Range
persistent identity: range1963752
display id: range1963752
version: 1
direction: direction/forward
top level: BBa_K091107
start: 26
end: 43
orientation: inline

Lux -35
type: SequenceAnnotation
persistent identity: annotation1963751
display id: annotation1963751
version: 1
top level: BBa_K091107
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K091107/annotation1963751/range1963751/1

https://synbiohub.org/public/igem/BBa_K091107/annotation1963751/range1963751/1
type: Range
persistent identity: range1963751
display id: range1963751
version: 1
direction: direction/forward
top level: BBa_K091107
start: 20
end: 25
orientation: inline

Lux Box
type: SequenceAnnotation
persistent identity: annotation1963750
display id: annotation1963750
version: 1
top level: BBa_K091107
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K091107/annotation1963750/range1963750/1

https://synbiohub.org/public/igem/BBa_K091107/annotation1963750/range1963750/1
type: Range
persistent identity: range1963750
display id: range1963750
version: 1
direction: direction/forward
top level: BBa_K091107
start: 1
end: 20
orientation: inline

lux pL
type: ComponentDefinition
persistent identity: BBa R0063
display id: BBa_R0063
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (luxR & HSL regulated -- lux pL)
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The lux cassette of V. fischeri contains a left and a right promoter. The left promoter gives weak constitutive expression of downstream genes.This expression is down-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription from Pr, repressing transcription from Pl
mutable notes: This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below.Includes most of Lux reulatory region, including the LuxR binding site which activates the right promoter. A putative LuxR autorepression binding site is also identified adjacent to the -10 site of the right promoter. This 2nd site has 55% identity with the first site. Putative inverted repeats (of size 18-27 bp) also exist between these two sites (not marked above), which may represent binding sites for other regulatory proteins. <img src="<bb_file>Image01.gif</bb_file>" width="614" height="362"> Unspecified.
mutable provenance: V. fischeri.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: start
sequence annotation: BBa_R0063
sequence annotation: Putative LuxR/HSL
sequence annotation: LuxR/HSL
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0063_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2054
display id: annotation2054
version: 1
top level: lux pL
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2054/range2054/1

https://synbiohub.org/public/igem/BBa_R0063/annotation2054/range2054/1
type: Range
persistent identity: range2054
display id: range2054
version: 1
direction: direction/forward
top level: lux pL
start: 128
end: 128
orientation: inline

BBa_R0063
type: SequenceAnnotation
persistent identity: annotation7071
display id: annotation7071
version: 1
top level: lux pL
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0063/annotation7071/range7071/1

https://synbiohub.org/public/igem/BBa_R0063/annotation7071/range7071/1
type: Range
persistent identity: range7071
display id: range7071
version: 1
direction: direction/forward
top level: lux pL
start: 1
end: 151
orientation: inline

Putative LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2055
display id: annotation2055
version: 1
top level: lux pL
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2055/range2055/1

https://synbiohub.org/public/igem/BBa_R0063/annotation2055/range2055/1
type: Range
persistent identity: range2055
display id: range2055
version: 1
direction: direction/forward
top level: lux pL
start: 130
end: 149
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2051
display id: annotation2051
version: 1
top level: lux pL
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2051/range2051/1

https://synbiohub.org/public/igem/BBa_R0063/annotation2051/range2051/1
type: Range
persistent identity: range2051
display id: range2051
version: 1
direction: direction/forward
top level: lux pL
start: 1
end: 20
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2053
display id: annotation2053
version: 1
top level: lux pL
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2053/range2053/1

https://synbiohub.org/public/igem/BBa_R0063/annotation2053/range2053/1
type: Range
persistent identity: range2053
display id: range2053
version: 1
direction: direction/forward
top level: lux pL
start: 89
end: 94
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2052
display id: annotation2052
version: 1
top level: lux pL
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2052/range2052/1

https://synbiohub.org/public/igem/BBa_R0063/annotation2052/range2052/1
type: Range
persistent identity: range2052
display id: range2052
version: 1
direction: direction/forward
top level: lux pL
start: 115
end: 122
orientation: inline