//function/biofuels
type: Collection
persistent identity: function biofuels
display id: function_biofuels
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_J15507
member: BBa_I725015
member: BBa_J15510
member: BBa_I72512
member: BBa_K294000
member: BBa_J15509
member: BBa_K118028
member: BBa_K118022
member: BBa_I725011
member: BBa_J15508
member: BBa_K144000
member: BBa_I725014
member: BBa_K1986001
member: BBa_I725013
member: HydA
member: BBa_K118023

BBa_K144000
type: ComponentDefinition
persistent identity: BBa K144000
display id: BBa_K144000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lignin Peroxidase A
creator: Caleb Dulaney
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:10:26Z
discontinued: false
dominant: false
group u list: _207_
m user id: 0
owner id: 2074
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part codes for the enzyme Lignin Peroxidase which initiates the breakdown of lignin, a complex biopolymer in biomass. The part is currently in testing, but should produce the active enzyme. It can be used in bioenergy projects to begin the process of making biofuels. Also, there has been some discussion about the possibility of using peroxidases to break down synthetic polymers.
mutable notes: This part is one of a family of highly homologous genes in P.C. It was nearly impossible to isolate the gene using primers specific for the coding sequence. It was necessary to use a sequential set of PCR primers specific for the upstream and downstream regions and then the specific coding sequence, respectively.
mutable provenance: cDNA of Phanerochaete Chrysosporium grown on Aspen Wood Culture at University of Wisconsin/USDA Forest Products Lab.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K144000_sequence/1

BBa_J15509
type: ComponentDefinition
persistent identity: BBa J15509
display id: BBa_J15509
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac+RBS+cex+RBS+cenA
creator: Kwabena O. Duedu, Chris French, Sahreena Lakhundi
created: 2013-11-25T12:00:00Z
modified: 2015-08-31T04:08:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _163_
m user id: 0
owner id: 14847
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Exo- and endo- glucanases from the cellulolytic bacterium Cellulomonas fimi under the E. coli lac promoter. Together with betaglucosidases, the bacterium degrades cellulose into D-glucose.
mutable notes: Cex is a GC rich part and may require extended denaturation in amplifying the gene.
mutable provenance: Cellulomonas fimi
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J15507
component: BBa_J15508
component: BBa_J33207
sequence annotation: BBa_J33207
sequence annotation: BBa_J15507
sequence annotation: BBa_J15508
sequence: https://synbiohub.org/public/igem/BBa_J15509_sequence/1

BBa_J15507
type: Component
persistent identity: component2371456
display id: component2371456
version: 1
top level: BBa_J15509
access: public
definition: BBa_J15507

BBa_J15508
type: Component
persistent identity: component2371462
display id: component2371462
version: 1
top level: BBa_J15509
access: public
definition: BBa_J15508

BBa_J33207
type: Component
persistent identity: component2371449
display id: component2371449
version: 1
top level: BBa_J15509
access: public
definition: https://synbiohub.org/public/igem/BBa_J33207/1

BBa_J33207
type: SequenceAnnotation
persistent identity: annotation2371449
display id: annotation2371449
version: 1
top level: BBa_J15509
location: https://synbiohub.org/public/igem/BBa_J15509/annotation2371449/range2371449/1
component: BBa_J33207

https://synbiohub.org/public/igem/BBa_J15509/annotation2371449/range2371449/1
type: Range
persistent identity: range2371449
display id: range2371449
version: 1
direction: direction/reverse
top level: BBa_J15509
start: 1
end: 600
orientation: inline

BBa_J15507
type: SequenceAnnotation
persistent identity: annotation2371456
display id: annotation2371456
version: 1
top level: BBa_J15509
location: https://synbiohub.org/public/igem/BBa_J15509/annotation2371456/range2371456/1
component: BBa_J15507

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type: Range
persistent identity: range2371456
display id: range2371456
version: 1
direction: direction/reverse
top level: BBa_J15509
start: 601
end: 2071
orientation: inline

BBa_J15508
type: SequenceAnnotation
persistent identity: annotation2371462
display id: annotation2371462
version: 1
top level: BBa_J15509
location: https://synbiohub.org/public/igem/BBa_J15509/annotation2371462/range2371462/1
component: BBa_J15508

https://synbiohub.org/public/igem/BBa_J15509/annotation2371462/range2371462/1
type: Range
persistent identity: range2371462
display id: range2371462
version: 1
direction: direction/reverse
top level: BBa_J15509
start: 2072
end: 3434
orientation: inline

BBa_I72512
type: ComponentDefinition
persistent identity: BBa I72512
display id: BBa_I72512
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Enoyl-coa hydratase
creator: Erin Dul
created: 2007-06-17T11:00:00Z
modified: 2015-08-31T04:07:54Z
discontinued: false
dominant: false
group u list: _106_
m user id: 0
owner id: 1824
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Enoly-coa hydratase from C. acetobutylicum. The sequence has been selected because it is part of an operon that has successfully been cloned into E. coli. It catalyzes the second step in butanol biosythesis. It has a His-tag added to its C terminus.
mutable notes: The sequence has had its codons optimized for E. coli and has had its restriction sites removed. The sequence has had its stop codon changed to TAATAA. It has had a His tag added to its c terminus.
mutable provenance: C. acetobutylicum genome. CAC_2712
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I72512_sequence/1

BBa_I725015
type: ComponentDefinition
persistent identity: BBa I725015
display id: BBa_I725015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Butanol dehydrogenase
creator: Erin Dul
created: 2007-06-17T11:00:00Z
modified: 2015-08-31T04:07:54Z
discontinued: false
dominant: false
group u list: _106_
m user id: 0
owner id: 1824
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Butanol dehydrogenase from C. acetobutylicum. This catalyzes the fifth step of butanol biosythesis. It has been cloned into e. coli previously and activity has been recovered.
mutable notes: This part has been optimized for synthesis in E. Coli and has had its restriction sites removed. It has had its GTG start codon mutated to ATG and its stop codon mutated to TAATAA. It has also had ad C terminal His tag added.
mutable provenance: C. acetobutylicum genome CAC_3298
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I725015_sequence/1

BBa_J15507
type: ComponentDefinition
persistent identity: BBa J15507
display id: BBa_J15507
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS+Cex (Exoglucanase)
creator: Kwabena O. Duedu
created: 2013-11-25T12:00:00Z
modified: 2015-08-31T04:08:33Z
discontinued: false
dominant: false
group u list: _163_
m user id: 0
owner id: 14847
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: RBS + Exoglucanase (cex, BBa_K118022) from the cellulolytic bacterium Cellulomonas fimi (accession M15824).
mutable notes: GC rich part. Extended denaturation stage in PCR with glycerol or commercial GC rich enhancers may be required
mutable provenance: Cellulomonas fimi exoglucanase cex (accession M15824) with RBS
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K118022
component: BBa_J15001
sequence annotation: BBa_K118022
sequence annotation: BBa_J15001
sequence: https://synbiohub.org/public/igem/BBa_J15507_sequence/1

BBa_K118022
type: Component
persistent identity: component2371436
display id: component2371436
version: 1
top level: BBa_J15507
access: public
definition: BBa_K118022

BBa_J15001
type: Component
persistent identity: component2371433
display id: component2371433
version: 1
top level: BBa_J15507
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_K118022
type: SequenceAnnotation
persistent identity: annotation2371436
display id: annotation2371436
version: 1
top level: BBa_J15507
location: https://synbiohub.org/public/igem/BBa_J15507/annotation2371436/range2371436/1
component: BBa_K118022

https://synbiohub.org/public/igem/BBa_J15507/annotation2371436/range2371436/1
type: Range
persistent identity: range2371436
display id: range2371436
version: 1
direction: direction/reverse
top level: BBa_J15507
start: 11
end: 1471
orientation: inline

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation2371433
display id: annotation2371433
version: 1
top level: BBa_J15507
location: https://synbiohub.org/public/igem/BBa_J15507/annotation2371433/range2371433/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_J15507/annotation2371433/range2371433/1
type: Range
persistent identity: range2371433
display id: range2371433
version: 1
direction: direction/reverse
top level: BBa_J15507
start: 1
end: 10
orientation: inline

HydA
type: ComponentDefinition
persistent identity: BBa M36596
display id: BBa_M36596
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Oxygen-tolerant hydrogenase (with RBS and terminator)
creator: Emma Marriott, Jessica Lam, Tatum Banayat
created: 2013-06-08T11:00:00Z
modified: 2015-05-08T01:14:05Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _848_
m user id: 0
owner id: 17308
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Hydrogenase is an enzyme that catalyzes the reversible oxidation of H2. This part encodes a hydrogenase actuator taken from the large subunit of the marine bacterium Hydrogenovibrius Marinus, which has been shown to be relatively tolerant to oxygen (Kim et. Al, 2011). This part consists of a strong bicistronic design RBS (found under BioFAB ID: pFAB1655), a codon-optimized HmMBH actuator gene taken from the sequence of the large subunit of hydrogenase found in hydrogenovibrius marinus (original sequence found under F2Z6J6 at uniprot.org), and a high efficiency transcriptional terminator (found under BBa_M36281 in the Registry of Standard Biological Parts). The mechanism of the part is relatively straightforward: Once induced with the PoPS signal, the actuator will initiate protein translation and express the hydrogenase enzyme. Our actuator was used in conjunction with a rhamnose sensor (e.g. BBa_M36284).
mutable notes: Considerations of the benefits and convenience of having a bicsistronic design led us to choose this option, in spite of the fact that the bicistronic design is targeted for E. Coli and has not yet been tested in S. Elongatus. Interestingly enough, although the part was originally designed for expression of the hydrogenase protein in cyanobacteria, it has so far only been proved for the expression of hydrogenase protein in E. Coli. In regards to the HmMBH actuator gene, the sequence was optimized to reduce repeats and the number of sites available to restriction enzymes.
mutable provenance: Bicistronic RBS: Mutalik VK, Guimaraes JC, Cambray G, Mai QA, Christoffersen MJ, Martin L, Yua A, Lam C, Rodriguez C, Bennett G, et al. Quantitative estimation of activity and quality for collections of functional genetic elements. Nat. Methods. 2013;10:347-353. BioFAB. (http://io.biofab.org:5000/simple/randomized_bds) Sequence for large subunit Hydrogenase: Nishihara H, Miyata Y, Miyashita Y, Bernhard M, Pohlmann A, et al. Analysis of the molecular species of hydrogenase in the cells of an obligately chemolithoautotrophic, marine hydrogen-oxidizing bacterium, Hydrogenovibrio marinus. Biosci Biotechnol Biochem. 2001;65:2780???2784. UnitPRO (http://www.uniprot.org/uniprot/F2Z6J6). High efficiency transcriptional terminator: Sunil Bodapati, Julia Joung, Daniel Esquivel-Reynoso. Part: BBa_M36281: Hard Information. Registry of Standard Biological Parts. (http://partsregistry.org/cgi/partsdb/part_info.cgi?part_name=BBa%20M36281)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_M36596
sequence annotation: BBa_M36595
sequence annotation: Hydrogenase Actuator
sequence annotation: BBa_M36281
sequence: https://synbiohub.org/public/igem/BBa_M36596_sequence/1

BBa_M36596
type: SequenceAnnotation
persistent identity: annotation2329340
display id: annotation2329340
version: 1
top level: HydA
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_M36596/annotation2329340/range2329340/1

https://synbiohub.org/public/igem/BBa_M36596/annotation2329340/range2329340/1
type: Range
persistent identity: range2329340
display id: range2329340
version: 1
direction: direction/reverse
top level: HydA
start: 94
end: 1926
orientation: inline

BBa_M36595
type: SequenceAnnotation
persistent identity: annotation2329338
display id: annotation2329338
version: 1
top level: HydA
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_M36596/annotation2329338/range2329338/1

https://synbiohub.org/public/igem/BBa_M36596/annotation2329338/range2329338/1
type: Range
persistent identity: range2329338
display id: range2329338
version: 1
direction: direction/reverse
top level: HydA
start: 1
end: 87
orientation: inline

Hydrogenase Actuator
type: SequenceAnnotation
persistent identity: annotation2329339
display id: annotation2329339
version: 1
top level: HydA
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_M36596/annotation2329339/range2329339/1

https://synbiohub.org/public/igem/BBa_M36596/annotation2329339/range2329339/1
type: Range
persistent identity: range2329339
display id: range2329339
version: 1
direction: direction/forward
top level: HydA
start: 94
end: 1926
orientation: inline

BBa_M36281
type: SequenceAnnotation
persistent identity: annotation2329341
display id: annotation2329341
version: 1
top level: HydA
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_M36596/annotation2329341/range2329341/1

https://synbiohub.org/public/igem/BBa_M36596/annotation2329341/range2329341/1
type: Range
persistent identity: range2329341
display id: range2329341
version: 1
direction: direction/reverse
top level: HydA
start: 1935
end: 2016
orientation: inline

BBa_K118022
type: ComponentDefinition
persistent identity: BBa K118022
display id: BBa_K118022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cex coding sequence encoding Cellulomonas fimi exoglucanase
creator: Andrew Hall
created: 2008-10-06T11:00:00Z
modified: 2015-05-08T01:09:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: The fungus ''Cellulomonas fimi'' uses an exoglucanase (from ''cex'') along with 3 endoglucanases in the degradation of cellulose into cellobiose, before use B-glucosidase to catalyse the conversion of cellobiose to D-glucose.
mutable notes: The original sequence had a PstI site for 781-786. We added the silent mutation CAG->CAA (Gln) by MABEL to remove this site.
mutable provenance: Purified ''Cellulomonas fimi'' genomic DNA.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: PstI site removed
sequence annotation: cex coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K118022_sequence/1

PstI site removed
type: SequenceAnnotation
persistent identity: annotation1996994
display id: annotation1996994
version: 1
top level: BBa_K118022
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K118022/annotation1996994/range1996994/1

https://synbiohub.org/public/igem/BBa_K118022/annotation1996994/range1996994/1
type: Range
persistent identity: range1996994
display id: range1996994
version: 1
direction: direction/forward
top level: BBa_K118022
start: 781
end: 786
orientation: inline

cex coding sequence
type: SequenceAnnotation
persistent identity: annotation1996993
display id: annotation1996993
version: 1
top level: BBa_K118022
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K118022/annotation1996993/range1996993/1

https://synbiohub.org/public/igem/BBa_K118022/annotation1996993/range1996993/1
type: Range
persistent identity: range1996993
display id: range1996993
version: 1
direction: direction/forward
top level: BBa_K118022
start: 1
end: 1458
orientation: inline

BBa_I725011
type: ComponentDefinition
persistent identity: BBa I725011
display id: BBa_I725011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B-hydroxy butyryl coA dehydrogenase
creator: Erin Dul
created: 2007-06-17T11:00:00Z
modified: 2015-08-31T04:07:54Z
discontinued: false
dominant: false
group u list: _106_
m user id: 0
owner id: 1824
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: B-hydroxybutyryl coa dehydrogenase from C. acetobutylicum. Catalyzes the first step in the conversion of aceto-acetylcoa into butanol. It is from an operon containing this gene as well as two other genes in the butanol production pathway. It has been successfully cloned into e. coli before and activity has been recovered. The sequence has C-terminal His-tag.
mutable notes: The sequence has been optimized for E. Coli and has had its restriction sites removed. A His tag has been added to the C terminus. The stop codon has been changed to TAATAA.
mutable provenance: Genomic sequence of Chlostridium acetobutylicum. CAC_2708.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I725011_sequence/1

BBa_I725014
type: ComponentDefinition
persistent identity: BBa I725014
display id: BBa_I725014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Butyraldehyde dehydrogenase
creator: Erin Dul
created: 2007-06-17T11:00:00Z
modified: 2015-08-31T04:07:54Z
discontinued: false
dominant: false
group u list: _106_
m user id: 0
owner id: 1824
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is the butyraldehyde dehydrogenase from C. acetobutylicum. It catalyzes the fourth step in the reaction of butanol biosythesis. Its is from the pSOL plasmid of C. acetobutylicum. It also has some alcohol dehydrogenase activity.
mutable notes: The sequence has been optimized for use in E. Coli and has had its restriction sites removed. It has had a c-terminal His tag added and its stop codon has been changed to TAATAA.
mutable provenance: pSOL plamid of C. acetobutylicum. CAP_0035
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I725014_sequence/1

BBa_I725013
type: ComponentDefinition
persistent identity: BBa I725013
display id: BBa_I725013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Butyryl CoA dehyrogenase
creator: Erin Dul
created: 2007-06-17T11:00:00Z
modified: 2015-08-31T04:07:54Z
discontinued: false
dominant: false
group u list: _106_
m user id: 0
owner id: 1824
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Butyryl coa dehyrogenase from C. acetobutylicum. The sequence has been selected because it is part of an operon that has successfully been cloned into E. coli. It catalyzes the third step in butanol biosythesis. It has a His-tag added to its C terminus.
mutable notes: The sequence has been optimized for use in E. Coli and has had its restriction sites removed. It has had a c-terminal His tag added and its stop codon has been changed to TAATAA.
mutable provenance: C. acetobutylicum genom. CAC_2712
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I725013_sequence/1

BBa_K294000
type: ComponentDefinition
persistent identity: BBa K294000
display id: BBa_K294000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: This is the coding sequence for the heat shock protein hsp15 from E. coli
creator: Bartholomew Canton
created: 2009-06-26T11:00:00Z
modified: 2015-05-08T01:11:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _397_
m user id: 0
owner id: 135
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This coding sequence produces a protein that is involved in the heat shock response of E. coli. We are using this part to regulate the activity of a heat shock promoter as part of a bacterial lava lamp.
mutable notes: We chose this heat shock protein because it operates well in multiple bacteria including E. coli and B. subtilis and our project involves both organisms.
mutable provenance: hsp15 is derived from the genome of E. coli K12. We retrieved the [http://BioCyc.org/ECOLI/NEW-IMAGE?type=GENE&object=G7743 sequence] from the Ecocyc database on June 26th 2009.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: hslR
sequence annotation: start codon
sequence: https://synbiohub.org/public/igem/BBa_K294000_sequence/1

hslR
type: SequenceAnnotation
persistent identity: annotation2006746
display id: annotation2006746
version: 1
top level: BBa_K294000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K294000/annotation2006746/range2006746/1

https://synbiohub.org/public/igem/BBa_K294000/annotation2006746/range2006746/1
type: Range
persistent identity: range2006746
display id: range2006746
version: 1
direction: direction/forward
top level: BBa_K294000
start: 1
end: 402
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2006750
display id: annotation2006750
version: 1
top level: BBa_K294000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K294000/annotation2006750/range2006750/1

https://synbiohub.org/public/igem/BBa_K294000/annotation2006750/range2006750/1
type: Range
persistent identity: range2006750
display id: range2006750
version: 1
direction: direction/forward
top level: BBa_K294000
start: 1
end: 3
orientation: inline

BBa_J15508
type: ComponentDefinition
persistent identity: BBa J15508
display id: BBa_J15508
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS+CenA (Endoglucanase)
creator: Kwabena O. Duedu
created: 2013-11-25T12:00:00Z
modified: 2015-08-31T04:08:33Z
discontinued: false
dominant: false
group u list: _163_
m user id: 0
owner id: 14847
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Endoglucanase A (CenA) from the cellulolytic bacterium Cellulomonas fimi (accession M15823) used in the degradation of cellulose into cellobiose, before using beta-glucosidase to catalyse the conversion of cellobiose to D-glucose.
mutable notes: No special conditions required
mutable provenance: Cellulomonas fimi (accession M15823)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J15001
component: BBa_K118023
sequence annotation: BBa_K118023
sequence annotation: BBa_J15001
sequence: https://synbiohub.org/public/igem/BBa_J15508_sequence/1

BBa_J15001
type: Component
persistent identity: component2371439
display id: component2371439
version: 1
top level: BBa_J15508
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_K118023
type: Component
persistent identity: component2371441
display id: component2371441
version: 1
top level: BBa_J15508
access: public
definition: BBa_K118023

BBa_K118023
type: SequenceAnnotation
persistent identity: annotation2371441
display id: annotation2371441
version: 1
top level: BBa_J15508
location: https://synbiohub.org/public/igem/BBa_J15508/annotation2371441/range2371441/1
component: BBa_K118023

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type: Range
persistent identity: range2371441
display id: range2371441
version: 1
direction: direction/reverse
top level: BBa_J15508
start: 11
end: 1363
orientation: inline

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation2371439
display id: annotation2371439
version: 1
top level: BBa_J15508
location: https://synbiohub.org/public/igem/BBa_J15508/annotation2371439/range2371439/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_J15508/annotation2371439/range2371439/1
type: Range
persistent identity: range2371439
display id: range2371439
version: 1
direction: direction/reverse
top level: BBa_J15508
start: 1
end: 10
orientation: inline

BBa_J15510
type: ComponentDefinition
persistent identity: BBa J15510
display id: BBa_J15510
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pspac+RBS+cex+RBS+cenA
creator: Kwabena O. Duedu, Chris French, Sahreena Lakhundi
created: 2013-11-25T12:00:00Z
modified: 2015-08-31T04:08:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _163_
m user id: 0
owner id: 14847
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Exo- and endo- glucanases from the cellulolytic bacterium Cellulomonas fimi under the E. coli spac promoter (BBa_J15506). Together with betaglucosidases, the bacterium degrades cellulose into D-glucose.
mutable notes: Cex is a GC rich part and requires extended denaturation with glycerol or commercial GC enhancers
mutable provenance: Cellulomonas fimi
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J15503
component: BBa_J15507
component: BBa_J15508
sequence annotation: BBa_J15508
sequence annotation: BBa_J15503
sequence annotation: BBa_J15507
sequence: https://synbiohub.org/public/igem/BBa_J15510_sequence/1

BBa_J15503
type: Component
persistent identity: component2371463
display id: component2371463
version: 1
top level: BBa_J15510
access: public
definition: https://synbiohub.org/public/igem/BBa_J15503/1

BBa_J15507
type: Component
persistent identity: component2371470
display id: component2371470
version: 1
top level: BBa_J15510
access: public
definition: BBa_J15507

BBa_J15508
type: Component
persistent identity: component2371476
display id: component2371476
version: 1
top level: BBa_J15510
access: public
definition: BBa_J15508

BBa_J15508
type: SequenceAnnotation
persistent identity: annotation2371476
display id: annotation2371476
version: 1
top level: BBa_J15510
location: https://synbiohub.org/public/igem/BBa_J15510/annotation2371476/range2371476/1
component: BBa_J15508

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type: Range
persistent identity: range2371476
display id: range2371476
version: 1
direction: direction/reverse
top level: BBa_J15510
start: 1756
end: 3118
orientation: inline

BBa_J15503
type: SequenceAnnotation
persistent identity: annotation2371463
display id: annotation2371463
version: 1
top level: BBa_J15510
location: https://synbiohub.org/public/igem/BBa_J15510/annotation2371463/range2371463/1
component: BBa_J15503

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type: Range
persistent identity: range2371463
display id: range2371463
version: 1
direction: direction/reverse
top level: BBa_J15510
start: 1
end: 284
orientation: inline

BBa_J15507
type: SequenceAnnotation
persistent identity: annotation2371470
display id: annotation2371470
version: 1
top level: BBa_J15510
location: https://synbiohub.org/public/igem/BBa_J15510/annotation2371470/range2371470/1
component: BBa_J15507

https://synbiohub.org/public/igem/BBa_J15510/annotation2371470/range2371470/1
type: Range
persistent identity: range2371470
display id: range2371470
version: 1
direction: direction/reverse
top level: BBa_J15510
start: 285
end: 1755
orientation: inline

BBa_K118023
type: ComponentDefinition
persistent identity: BBa K118023
display id: BBa_K118023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cenA coding sequence encoding Cellulomonas fimi endoglucanase A
creator: Andrew Hall
created: 2008-10-06T11:00:00Z
modified: 2015-05-08T01:09:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: The cellulolytic bacterium ''Cellulomonas fimi'' uses 3 endoglucanase (including CenA) and an exoglucanase in the degradation of cellulose into cellobiose, before use B-glucosidase to catalyse the conversion of cellobiose to D-glucose.
mutable notes: No special considerations.
mutable provenance: Purified ''Cellulomonas fimi'' genomic DNA.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cenA coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K118023_sequence/1

cenA coding sequence
type: SequenceAnnotation
persistent identity: annotation1996991
display id: annotation1996991
version: 1
top level: BBa_K118023
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K118023/annotation1996991/range1996991/1

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type: Range
persistent identity: range1996991
display id: range1996991
version: 1
direction: direction/forward
top level: BBa_K118023
start: 1
end: 1350
orientation: inline

BBa_K118028
type: ComponentDefinition
persistent identity: BBa K118028
display id: BBa_K118028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: beta-glucosidase gene bglX (chu_2268) from Cytophaga hutchinsonii
creator: Chris French
created: 2008-10-22T11:00:00Z
modified: 2015-05-08T01:09:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _192_
m user id: 0
owner id: 837
owning group id: 163
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is a beta-glucosidase gene (bglX, locus chu_2268) from the genome sequence of the cellulose-degrading Gram negative bacterium Cytophaga hutchinsonii.
mutable notes: No special considerations.
mutable provenance: Genome sequence of the cellulose-degrading Gram negative bacterium Cytophaga hutchinsonii.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: bglX coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K118028_sequence/1

bglX coding sequence
type: SequenceAnnotation
persistent identity: annotation1985223
display id: annotation1985223
version: 1
top level: BBa_K118028
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K118028/annotation1985223/range1985223/1

https://synbiohub.org/public/igem/BBa_K118028/annotation1985223/range1985223/1
type: Range
persistent identity: range1985223
display id: range1985223
version: 1
direction: direction/forward
top level: BBa_K118028
start: 1
end: 2277
orientation: inline

BBa_K1986001
type: ComponentDefinition
persistent identity: BBa K1986001
display id: BBa_K1986001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Optimizer in lipid production
creator: Carolina Guadalupe Cruz N????ez, Alejandro Alarc??n del Carmen, Franklin Cruz Villegas
created: 2016-10-13T11:00:00Z
modified: 2016-10-30T07:22:01Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2453_
m user id: 25309
owner id: 30074
owning group id: 9
status: status/Planning
bookmark: true
mutable description: edit later description
mutable notes: edit consdierations
mutable provenance: edit soruce
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K1986001_sequence/1