BBa_R0010 Promoter (RBS+) with BBa_J04671 (EYFP, RBS-, LVA+, B0015)
This is a construct with the Promoter Sequence BBa_R0010 and the Reporter Construct BBa_J04671 (EYFP, RBS-, LVA+, B0015)
B0034.E0036.B0015 output device with asv tagged yfp
Jay Blumling, Debra Lin, Madeleine Sheldon-dante, Fred Tan, MIT SMUG
Threshold: Reporter module (regulated by cI)
Part of the thresholding device (works with j07036, j07038/j07039/j07040). This encodes the reporter gene that is regulated by the cI repressor (coded by j07036). The amount of cI itself is regulated by j07038/39/40 that code for antisense mRNA to the cI transcript, and hence repress translation of cI (and activate j07037)
B0034.E0034.B0015 output device with aav tagged yfp
This part was constructed from registry biobricks.
This part was designed to include an AAV degradation tag.
medium RBS, YFP (-LVA), double terminator
Venus YFP with RBS and forward terminator
Combines BBa_B0034 RBS (Elowitz 1999) with BBa_J06600 Venus YFP with forward terminator
Test constructs involving this part had no fluorescence observed. Sequencing returned nothing resembling the expected part.
mCherry, bacterial with RBS and forward terminator
Combines BBa_B0015 forward terminator with BBa_J06602 mCherry, bacterial with RBS
rbs-GFP(LVA)-terminators (Backbone: pSB1A2)
This part is a generator that lacks a promoter. The GFP has a LVA degradation tail. Upon expression, the GFP will give green fluorescence color.
Built by Josh as an intermediate in screening plasmid construction.
This part was constructed from parts available from the registry.
Combines BBa_B0034 RBS (Elowitz 1999) with BBa_J06601 mCherry with forward terminator
This part was designed to incorporate CFP with an LVA degradation tag.
Green fluorescent protein (E0240) is constitutively produced thanks to a constitutive promoter (J23119).
This part was build following the standard construction protocol.
J23119 is an E.coli constitutive promoter. The GFP sequence included in E0240 is a green fluorescent protein derived from jellyfish Aequeora victoria wild type GFP.
This part contains a green fluorescence protein (GFP) under control of a CMV promoter and its double terminator (BBa_I712669 + BBa_B0014). This part is reliable to use when we want to know if a gene is inserted into a eukaryotic cell. For example we can use it when we design a recombinant virus. In fact we inserted this bio-brick into the synthetic virus; finally we are able to see if the virus can infect the targeted cells. This bio-brick already contains a terminator, so it is ready to use.
Part:BBa_I712669, Designed by Rok Gaber Group: iGEM07_Ljubljana (2007-10-24) Part:BBa_B0014, Designed by Reshma Shetty Group: Registry (2003-07-16)
mCherry, bacterial (LVA+) with RBS and forward terminator
Combines BBa_B0015 forward terminator with BBa_J06603 mCherry, bacterial (LVA+) with RBS