//classic/regulatory/other
type: Collection
persistent identity: classic regulatory other
display id: classic_regulatory_other
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K1486013
member: pBad/araC
member: BBa_K332033
member: BBa_K1486005
member: recAp
member: AnIn
member: BBa_K731201
member: BBa_K993000
member: BBa_K1486012
member: BBa_K1486055
member: Ara
member: BBa_K1468004
member: BBa_K332031
member: BBa_K1486015
member: BBa_K1486041
member: BBa_K1486011
member: BBa_K1486054
member: BBa_K1300000
member: Pgad
member: BBa_K772009
member: BBa_K1486002
member: BBa_K993002
member: BBa_K772004
member: BBa_K1486008
member: BBa_K1486040
member: BBa_K1486010
member: BBa_K1486001
member: BBa_K1486014
member: BBa_K332032
member: BBa_K993003
member: BBa_K772005
member: BBa_K1486009
member: recNp
member: BBa_K1486043
member: BBa_K1486056

AnIn
type: ComponentDefinition
persistent identity: BBa K772007
display id: BBa_K772007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP - LasR anchor-inducer protein complex
creator: Mustafa Elitok
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1024_
m user id: 0
owner id: 9573
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part contains a green fluorescent protein (GFP) as a non-reactive chemical compound (anchor) in order to neutralize the active reactive fixed to it, an inducer regulatory protein (LasR is responsible to induce pLas promoter in the presence of 6HSLO3 which is a chemical compound sythesized by an enzyme that LasI gene codes.) and a TEV protease cleavage site between them to assemble these two proteins. BBa_772001 and BBa_K772002 are assembled to be used as the trigger of detection in the whole system which ends with the intracellular transfection (or activation) of TEV protease. After the activation, it is planned that TEV protease will cleave this anchor-inducer protein complex to release free the reporter. In conclusion, in this system, the inducer would activate a transcription pathway on the targeted inducible gene or the reporter would indicate that there is a change in the whole system.
mutable notes: Cleavage site, LasR and GFP sequences are gathered from Registry.
mutable provenance: Note that there is an RBS sequence just before the protein sequence in order to eliminate the need of addition of it.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: GFP
sequence annotation: RBS
sequence annotation: LasR
sequence annotation: Double Terminator
sequence annotation: Anchor - Inducer
sequence annotation: TEV cleavage site
sequence: https://synbiohub.org/public/igem/BBa_K772007_sequence/1

GFP
type: SequenceAnnotation
persistent identity: annotation2203899
display id: annotation2203899
version: 1
top level: AnIn
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772007/annotation2203899/range2203899/1

https://synbiohub.org/public/igem/BBa_K772007/annotation2203899/range2203899/1
type: Range
persistent identity: range2203899
display id: range2203899
version: 1
direction: direction/forward
top level: AnIn
start: 21
end: 741
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2203898
display id: annotation2203898
version: 1
top level: AnIn
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K772007/annotation2203898/range2203898/1

https://synbiohub.org/public/igem/BBa_K772007/annotation2203898/range2203898/1
type: Range
persistent identity: range2203898
display id: range2203898
version: 1
direction: direction/forward
top level: AnIn
start: 1
end: 20
orientation: inline

LasR
type: SequenceAnnotation
persistent identity: annotation2203901
display id: annotation2203901
version: 1
top level: AnIn
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772007/annotation2203901/range2203901/1

https://synbiohub.org/public/igem/BBa_K772007/annotation2203901/range2203901/1
type: Range
persistent identity: range2203901
display id: range2203901
version: 1
direction: direction/forward
top level: AnIn
start: 880
end: 1636
orientation: inline

Double Terminator
type: SequenceAnnotation
persistent identity: annotation2203909
display id: annotation2203909
version: 1
top level: AnIn
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K772007/annotation2203909/range2203909/1

https://synbiohub.org/public/igem/BBa_K772007/annotation2203909/range2203909/1
type: Range
persistent identity: range2203909
display id: range2203909
version: 1
direction: direction/forward
top level: AnIn
start: 1636
end: 1720
orientation: inline

Anchor - Inducer
type: SequenceAnnotation
persistent identity: annotation2203902
display id: annotation2203902
version: 1
top level: AnIn
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K772007/annotation2203902/range2203902/1

https://synbiohub.org/public/igem/BBa_K772007/annotation2203902/range2203902/1
type: Range
persistent identity: range2203902
display id: range2203902
version: 1
direction: direction/forward
top level: AnIn
start: 21
end: 1636
orientation: inline

TEV cleavage site
type: SequenceAnnotation
persistent identity: annotation2203900
display id: annotation2203900
version: 1
top level: AnIn
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K772007/annotation2203900/range2203900/1

https://synbiohub.org/public/igem/BBa_K772007/annotation2203900/range2203900/1
type: Range
persistent identity: range2203900
display id: range2203900
version: 1
direction: direction/forward
top level: AnIn
start: 782
end: 871
orientation: inline

BBa_K772005
type: ComponentDefinition
persistent identity: BBa K772005
display id: BBa_K772005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AHL generator: LasI
creator: Mustafa Elitok
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
group u list: _1024_
m user id: 0
owner id: 9573
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Quorum sensing is the mechanism of communication between bacteria via synthesizing and detecting chemical compounds in the media. With these mediators, the promoters and their yield can be tuned or regulated. This part represents an alternative quorum sensing sender device which is designed to induce PoPS production in the parts including pLas such as BBa_K772004. LasI is an enzyme generator gene sequences which synthesizes 6HSLO3 (AHL) in order to induce a PoPS response in the pLas promoter. pLas promoter works in the presence of both 6HSLO3 and LasR protein.
mutable notes: Note that there is an RBS sequence just before LasI; in order to be easily used with quorum sensing receiver devices such as BBa_F2620 or BBa_K772004.
mutable provenance: LasI sequence is gathered from Registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: pLac
sequence annotation: RBS
sequence annotation: Terminator
sequence annotation: LasI
sequence annotation: LacI
sequence: https://synbiohub.org/public/igem/BBa_K772005_sequence/1

pLac
type: SequenceAnnotation
persistent identity: annotation2203713
display id: annotation2203713
version: 1
top level: BBa_K772005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K772005/annotation2203713/range2203713/1

https://synbiohub.org/public/igem/BBa_K772005/annotation2203713/range2203713/1
type: Range
persistent identity: range2203713
display id: range2203713
version: 1
direction: direction/forward
top level: BBa_K772005
start: 1133
end: 1331
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2203714
display id: annotation2203714
version: 1
top level: BBa_K772005
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K772005/annotation2203714/range2203714/1

https://synbiohub.org/public/igem/BBa_K772005/annotation2203714/range2203714/1
type: Range
persistent identity: range2203714
display id: range2203714
version: 1
direction: direction/forward
top level: BBa_K772005
start: 1332
end: 1352
orientation: inline

Terminator
type: SequenceAnnotation
persistent identity: annotation2203715
display id: annotation2203715
version: 1
top level: BBa_K772005
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K772005/annotation2203715/range2203715/1

https://synbiohub.org/public/igem/BBa_K772005/annotation2203715/range2203715/1
type: Range
persistent identity: range2203715
display id: range2203715
version: 1
direction: direction/forward
top level: BBa_K772005
start: 1969
end: 2098
orientation: inline

LasI
type: SequenceAnnotation
persistent identity: annotation2329745
display id: annotation2329745
version: 1
top level: BBa_K772005
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K772005/annotation2329745/range2329745/1

https://synbiohub.org/public/igem/BBa_K772005/annotation2329745/range2329745/1
type: Range
persistent identity: range2329745
display id: range2329745
version: 1
direction: direction/forward
top level: BBa_K772005
start: 1353
end: 1968
orientation: inline

LacI
type: SequenceAnnotation
persistent identity: annotation2203712
display id: annotation2203712
version: 1
top level: BBa_K772005
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772005/annotation2203712/range2203712/1

https://synbiohub.org/public/igem/BBa_K772005/annotation2203712/range2203712/1
type: Range
persistent identity: range2203712
display id: range2203712
version: 1
direction: direction/forward
top level: BBa_K772005
start: 1
end: 1132
orientation: inline

BBa_K1486041
type: ComponentDefinition
persistent identity: BBa K1486041
display id: BBa_K1486041
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFPC + CpxR
creator: Ione Pla Puigsubir??
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_K1486000
component: BBa_K1486037
component: BBa_I0500
component: BBa_K1486039
sequence annotation: BBa_K1486039
sequence annotation: BBa_K1486000
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486037
sequence annotation: BBa_I0500
sequence: https://synbiohub.org/public/igem/BBa_K1486041_sequence/1

BBa_B0034
type: Component
persistent identity: component2387211
display id: component2387211
version: 1
top level: BBa_K1486041
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486000
type: Component
persistent identity: component2387217
display id: component2387217
version: 1
top level: BBa_K1486041
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_K1486037
type: Component
persistent identity: component2387213
display id: component2387213
version: 1
top level: BBa_K1486041
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486037/1

BBa_I0500
type: Component
persistent identity: component2387209
display id: component2387209
version: 1
top level: BBa_K1486041
access: public
definition: pBad/araC

BBa_K1486039
type: Component
persistent identity: component2387212
display id: component2387212
version: 1
top level: BBa_K1486041
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486039/1

BBa_K1486039
type: SequenceAnnotation
persistent identity: annotation2387212
display id: annotation2387212
version: 1
top level: BBa_K1486041
location: https://synbiohub.org/public/igem/BBa_K1486041/annotation2387212/range2387212/1
component: BBa_K1486039

https://synbiohub.org/public/igem/BBa_K1486041/annotation2387212/range2387212/1
type: Range
persistent identity: range2387212
display id: range2387212
version: 1
direction: direction/reverse
top level: BBa_K1486041
start: 1223
end: 1294
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2387217
display id: annotation2387217
version: 1
top level: BBa_K1486041
location: https://synbiohub.org/public/igem/BBa_K1486041/annotation2387217/range2387217/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486041/annotation2387217/range2387217/1
type: Range
persistent identity: range2387217
display id: range2387217
version: 1
direction: direction/reverse
top level: BBa_K1486041
start: 1334
end: 2035
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2387211
display id: annotation2387211
version: 1
top level: BBa_K1486041
location: https://synbiohub.org/public/igem/BBa_K1486041/annotation2387211/range2387211/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486041/annotation2387211/range2387211/1
type: Range
persistent identity: range2387211
display id: range2387211
version: 1
direction: direction/reverse
top level: BBa_K1486041
start: 1211
end: 1222
orientation: inline

BBa_K1486037
type: SequenceAnnotation
persistent identity: annotation2387213
display id: annotation2387213
version: 1
top level: BBa_K1486041
location: https://synbiohub.org/public/igem/BBa_K1486041/annotation2387213/range2387213/1
component: BBa_K1486037

https://synbiohub.org/public/igem/BBa_K1486041/annotation2387213/range2387213/1
type: Range
persistent identity: range2387213
display id: range2387213
version: 1
direction: direction/reverse
top level: BBa_K1486041
start: 1295
end: 1333
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2387209
display id: annotation2387209
version: 1
top level: BBa_K1486041
location: https://synbiohub.org/public/igem/BBa_K1486041/annotation2387209/range2387209/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486041/annotation2387209/range2387209/1
type: Range
persistent identity: range2387209
display id: range2387209
version: 1
direction: direction/reverse
top level: BBa_K1486041
start: 1
end: 1210
orientation: inline

BBa_K332032
type: ComponentDefinition
persistent identity: BBa K332032
display id: BBa_K332032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive promoter+37℃ induced RBS+tetR+double terminator+Ptet+RBS+GFP+double terminator
creator: KUO, CHIA-CHUN
created: 2010-10-21T11:00:00Z
modified: 2015-05-08T01:12:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _452_
m user id: 0
owner id: 7059
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: We want to utilize a temperature-regulated mechanism to control the expression period of a crystal protein. Therefore, we took advantage of the interaction between 37?? C induced RBS, tetR gene and pTet promoter to create the temperature regulating circuit. The following is a description of our prescribed strategy. First, we use the constitutive promoter, 37?? C induced RBS (K115002), the tetR gene and terminators. We will call this part the ???Regulator???.
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_R0040
component: BBa_K332031
component: BBa_B0034
component: BBa_E0040
component: BBa_J61048
sequence annotation: BBa_B0034
sequence annotation: BBa_R0040
sequence annotation: BBa_E0040
sequence annotation: BBa_K332031
sequence annotation: BBa_J61048
sequence: https://synbiohub.org/public/igem/BBa_K332032_sequence/1

BBa_R0040
type: Component
persistent identity: component2273247
display id: component2273247
version: 1
top level: BBa_K332032
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_K332031
type: Component
persistent identity: component2273246
display id: component2273246
version: 1
top level: BBa_K332032
access: public
definition: BBa_K332031

BBa_B0034
type: Component
persistent identity: component2273253
display id: component2273253
version: 1
top level: BBa_K332032
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0040
type: Component
persistent identity: component2273255
display id: component2273255
version: 1
top level: BBa_K332032
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_J61048
type: Component
persistent identity: component2273256
display id: component2273256
version: 1
top level: BBa_K332032
access: public
definition: https://synbiohub.org/public/igem/BBa_J61048/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2273253
display id: annotation2273253
version: 1
top level: BBa_K332032
location: https://synbiohub.org/public/igem/BBa_K332032/annotation2273253/range2273253/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K332032/annotation2273253/range2273253/1
type: Range
persistent identity: range2273253
display id: range2273253
version: 1
direction: direction/reverse
top level: BBa_K332032
start: 978
end: 989
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2273247
display id: annotation2273247
version: 1
top level: BBa_K332032
location: https://synbiohub.org/public/igem/BBa_K332032/annotation2273247/range2273247/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K332032/annotation2273247/range2273247/1
type: Range
persistent identity: range2273247
display id: range2273247
version: 1
direction: direction/forward
top level: BBa_K332032
start: 916
end: 969
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2273255
display id: annotation2273255
version: 1
top level: BBa_K332032
location: https://synbiohub.org/public/igem/BBa_K332032/annotation2273255/range2273255/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K332032/annotation2273255/range2273255/1
type: Range
persistent identity: range2273255
display id: range2273255
version: 1
direction: direction/reverse
top level: BBa_K332032
start: 996
end: 1715
orientation: inline

BBa_K332031
type: SequenceAnnotation
persistent identity: annotation2273246
display id: annotation2273246
version: 1
top level: BBa_K332032
location: https://synbiohub.org/public/igem/BBa_K332032/annotation2273246/range2273246/1
component: BBa_K332031

https://synbiohub.org/public/igem/BBa_K332032/annotation2273246/range2273246/1
type: Range
persistent identity: range2273246
display id: range2273246
version: 1
direction: direction/reverse
top level: BBa_K332032
start: 1
end: 907
orientation: inline

BBa_J61048
type: SequenceAnnotation
persistent identity: annotation2273256
display id: annotation2273256
version: 1
top level: BBa_K332032
location: https://synbiohub.org/public/igem/BBa_K332032/annotation2273256/range2273256/1
component: BBa_J61048

https://synbiohub.org/public/igem/BBa_K332032/annotation2273256/range2273256/1
type: Range
persistent identity: range2273256
display id: range2273256
version: 1
direction: direction/reverse
top level: BBa_K332032
start: 1724
end: 1836
orientation: inline

BBa_K1486043
type: ComponentDefinition
persistent identity: BBa K1486043
display id: BBa_K1486043
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Leucine Zipper + split rLuc
creator: EPFL iGem team 2014
created: 2014-10-01T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: To edit. Pense ?? rajouter les linkers, je ne sais pas s'ils sont de igem ou s'il faut ajouter la s??quence ?? la registry. Check pour tous tes biobricks et dis-moi, please.
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486042
component: BBa_I0500
component: BBa_B0034
component: BBa_K1486020
component: BBa_K1486019
sequence annotation: BBa_K1486042
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486020
sequence annotation: BBa_K1486019
sequence annotation: BBa_I0500
sequence: https://synbiohub.org/public/igem/BBa_K1486043_sequence/1

BBa_K1486042
type: Component
persistent identity: component2392177
display id: component2392177
version: 1
top level: BBa_K1486043
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486042/1

BBa_K1486042
type: Component
persistent identity: component2392173
display id: component2392173
version: 1
top level: BBa_K1486043
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486042/1

BBa_I0500
type: Component
persistent identity: component2392170
display id: component2392170
version: 1
top level: BBa_K1486043
access: public
definition: pBad/araC

BBa_B0034
type: Component
persistent identity: component2392172
display id: component2392172
version: 1
top level: BBa_K1486043
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486020
type: Component
persistent identity: component2392178
display id: component2392178
version: 1
top level: BBa_K1486043
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486020/1

BBa_B0034
type: Component
persistent identity: component2392176
display id: component2392176
version: 1
top level: BBa_K1486043
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486019
type: Component
persistent identity: component2392174
display id: component2392174
version: 1
top level: BBa_K1486043
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486019/1

BBa_K1486042
type: SequenceAnnotation
persistent identity: annotation2392177
display id: annotation2392177
version: 1
top level: BBa_K1486043
location: https://synbiohub.org/public/igem/BBa_K1486043/annotation2392177/range2392177/1
component: BBa_K1486042

https://synbiohub.org/public/igem/BBa_K1486043/annotation2392177/range2392177/1
type: Range
persistent identity: range2392177
display id: range2392177
version: 1
direction: direction/reverse
top level: BBa_K1486043
start: 1715
end: 1864
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2392172
display id: annotation2392172
version: 1
top level: BBa_K1486043
location: https://synbiohub.org/public/igem/BBa_K1486043/annotation2392172/range2392172/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486043/annotation2392172/range2392172/1
type: Range
persistent identity: range2392172
display id: range2392172
version: 1
direction: direction/reverse
top level: BBa_K1486043
start: 1211
end: 1222
orientation: inline

BBa_K1486020
type: SequenceAnnotation
persistent identity: annotation2392178
display id: annotation2392178
version: 1
top level: BBa_K1486043
location: https://synbiohub.org/public/igem/BBa_K1486043/annotation2392178/range2392178/1
component: BBa_K1486020

https://synbiohub.org/public/igem/BBa_K1486043/annotation2392178/range2392178/1
type: Range
persistent identity: range2392178
display id: range2392178
version: 1
direction: direction/reverse
top level: BBa_K1486043
start: 1865
end: 2470
orientation: inline

BBa_K1486042
type: SequenceAnnotation
persistent identity: annotation2392173
display id: annotation2392173
version: 1
top level: BBa_K1486043
location: https://synbiohub.org/public/igem/BBa_K1486043/annotation2392173/range2392173/1
component: BBa_K1486042

https://synbiohub.org/public/igem/BBa_K1486043/annotation2392173/range2392173/1
type: Range
persistent identity: range2392173
display id: range2392173
version: 1
direction: direction/reverse
top level: BBa_K1486043
start: 1223
end: 1372
orientation: inline

BBa_K1486019
type: SequenceAnnotation
persistent identity: annotation2392174
display id: annotation2392174
version: 1
top level: BBa_K1486043
location: https://synbiohub.org/public/igem/BBa_K1486043/annotation2392174/range2392174/1
component: BBa_K1486019

https://synbiohub.org/public/igem/BBa_K1486043/annotation2392174/range2392174/1
type: Range
persistent identity: range2392174
display id: range2392174
version: 1
direction: direction/reverse
top level: BBa_K1486043
start: 1373
end: 1702
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2392170
display id: annotation2392170
version: 1
top level: BBa_K1486043
location: https://synbiohub.org/public/igem/BBa_K1486043/annotation2392170/range2392170/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486043/annotation2392170/range2392170/1
type: Range
persistent identity: range2392170
display id: range2392170
version: 1
direction: direction/reverse
top level: BBa_K1486043
start: 1
end: 1210
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2392176
display id: annotation2392176
version: 1
top level: BBa_K1486043
location: https://synbiohub.org/public/igem/BBa_K1486043/annotation2392176/range2392176/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486043/annotation2392176/range2392176/1
type: Range
persistent identity: range2392176
display id: range2392176
version: 1
direction: direction/reverse
top level: BBa_K1486043
start: 1703
end: 1714
orientation: inline

BBa_K993003
type: ComponentDefinition
persistent identity: BBa K993003
display id: BBa_K993003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Urease generator with ROSE thermometer
creator: FURKAN SACİT CEYLAN
created: 2013-06-13T11:00:00Z
modified: 2015-05-08T01:13:51Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1290_
m user id: 0
owner id: 11155
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Repression of heat shock gene expression (ROSE) element The repression of heat shock gene expression (ROSE) element is an RNA element found in the 5' UTR of some heat shock protein's mRNAs. The ROSE element is an RNA thermometer that negatively regulates heat shock gene expression. The secondary structure is thought to be altered by temperature, thus it is an RNA thermometer. This structure blocks access to the ribosome binding site at normal temperatures. During heat shock however, the structure changes freeing the ribosome binding site and allowing expression to occur.[2][3] A partial structure of this RNA element has been determined using NMR.[1] ROSE1 and ROSEAT2 are specific examples of ROSE elements.[4] ROSE1 is found in Bradyrhizobium japonicum whereas ROSEAT2 is a closely related element from Agrobacterium tumefaciens. The two RNA elements have similar secondary structures with ROSE1 having an extra hairpin.[4] All ROSE elements contain a characteristic 'bulged G' opposite the Shine-Dalgarno sequence binding site, without this nucleotide the RNA thermometer loses its temperature-sensitivity.[5]
mutable notes: Note that RNA thermometer actually works as an RBS sequence. Therefore, this part does not include an additional ribosome binding site. Also, this part does not contain an terminator sequence, but a simple stop codon. The RFP should be translated continuously.
mutable provenance: AATTTTGTCAAAATAATTTTATTGACAACGTCTTATTAACGTTGATATAATTTAAATTTTTTTTGACAAAAATGGGCTCGTGTTGTACAATAAATGTGCCGCGACAAGCGGTCCGGGCGCCCTAGGGGCCCGGCGGAGACGGGCGCCGGAGGTGTCCGACGCCTGCTCGTCAAGTTCTTGCTCCTTGGAGGATATGAAGATAAACAGGCAGCAGTACGCGGAGAGCTACGGGCCGACGGTGGGGGACAGGGTGAGGCTGGCGGACACGGACCTGGGGGAGGTGGAGAAGGACTACTACTACCTGGGGGACGAGGTGAACTTCGGGGGGGGGAAGGTGCTGAGGGAGGGGATGGGGGAGAACGGGACGTACACGAGGACGGAGAACGTGTTGGACCTGCTGCTGACGAACGCGCTGATATTGGACTACACGGGGATATACAAGGCGGACATAGGGGTGAAGGACGGGTACATAGTGGGGATAGGGAAGGGGGGGAACCCGGACATAATGGACGGGGTGACGCCGAACATGATAGTGGGGACGGCGACGGAGGTGATAGCGGCGGAGGGGAAGATAGTGACGGCGGGGGGGATAGACACGCACGTGCACTTCATAAACCCGGACCAGGTGGACGTGGCGCTGGCGAACGGGATAACGACGCTGTTCGGGGGGGGGACGGGGCCGGCGGAGGGGAGCAAGGCGACGACGGTGACGCCGGGGCCGTGGAACATAGAGAAGATGCTGAAGAGCACGGAGGGGCTGCCGATAAACGTGGGGATACTGGGGAAGGGGCACGGGAGCAGCATAGCGCCGATAATGGAGCAGATAGACGCGGGGGCGGCGGGGCTGAAGATACACGAGGACTGGGGGGCGACGCCGGCGAGCATAGACAGGAGCCTGACGGTGGCGGACGAGGCGGACGTGCAGGTGGCGATACACAGCGACACGCTGAACGAGGCGGGGTTCTTGGAGGACACGGTGAGGGCGATAAACGGGAGGGTGATACACAGCTTCCACGTGGAGGGGGCGGGGGGGGGGCACGCGCCGGACATAATGGCGATGGCGGGGCACCCGAACGTGCTGCCGAGCAGCACGAACCCGACGAGGCCGTTCACGGTGAACACGATAGACGAGCACTTGGACATGCTGATGGTGTGCCACCACCTGAAGCAGAACATACCGGAGGACGTGGCGTTCGCGGACAGCAGGATAAGGCCGGAGACGATAGCGGCGGAGGACATACTGCACGACCTGGGGATAATAAGCATGATGAGCACGGACGCGCTGGCGATGGGGAGGGCGGGGGAGATGGTGCTGAGGACGTGGCAGACGGCGGACAAGATGAAGAAGCAGAGGGGGCCGCTGGCGGAGGAGAAGAACGGGAGCGACAACTTCAGGCTGAAGAGGTACGTGAGCAAGTACACGATAAACCCGGCGATAGCGCAGGGGATGGCGCACGAGGTGGGGAGCATAGAGGAGGGGAAGTTCGCGGACCTGGTGCTGTGGGAGCCGAAGTTCTTCGGGGTGAAGGCGGACAGGGTGATAAAGGGGGGGATAATAGCGTACGCGCAGATAGGGGACCCGAGCGCGAGCATACCGACGCCGCAGCCGGTGATGGGGAGGAGGATGTACGGGACGGTGGGGGACCTGATACACGACACGAACATAACGTTCATGAGCAAGAGCAGCATACAGCAGGGGGTGCCGGCGAAGCTGGGGCTGAAGAGGAGGATAGGGACGGTGAAGAATTGTCGAAACATAGGGAAGAAGGACATGAAGTGGAACGACGTGACGACGGACATAGACATAAACCCGGAGACGTACGAGGTGAAGGTGGACGGGGAGGTGCTGACGTGCGAGCCGGTGAAGGAGCTGCCGATGGCGCAGAGGTACTTCCTGTTCTAATCGCTGGGACGCCCGCCCCTAGAAAAGGAGGACTAAAAATGAGCAACAACAACTACATAGTGCCGGGGGAGTACAGGGTGGCGGAGGGGGAGATAGA…
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: Stop Codon
sequence annotation: pVeg2
sequence annotation: Start Codon
sequence annotation: RBS
sequence annotation: Urease B
sequence annotation: ROSE Thermometer
sequence annotation: Insulator
sequence annotation: Urease C
sequence annotation: Urease A
sequence: https://synbiohub.org/public/igem/BBa_K993003_sequence/1

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2329469
display id: annotation2329469
version: 1
top level: BBa_K993003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329469/range2329469/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329469/range2329469/1
type: Range
persistent identity: range2329469
display id: range2329469
version: 1
direction: direction/forward
top level: BBa_K993003
start: 1901
end: 1903
orientation: inline

pVeg2
type: SequenceAnnotation
persistent identity: annotation2329447
display id: annotation2329447
version: 1
top level: BBa_K993003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329447/range2329447/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329447/range2329447/1
type: Range
persistent identity: range2329447
display id: range2329447
version: 1
direction: direction/forward
top level: BBa_K993003
start: 1
end: 97
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation2329467
display id: annotation2329467
version: 1
top level: BBa_K993003
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329467/range2329467/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329467/range2329467/1
type: Range
persistent identity: range2329467
display id: range2329467
version: 1
direction: direction/forward
top level: BBa_K993003
start: 1942
end: 1944
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2329471
display id: annotation2329471
version: 1
top level: BBa_K993003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329471/range2329471/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329471/range2329471/1
type: Range
persistent identity: range2329471
display id: range2329471
version: 1
direction: direction/forward
top level: BBa_K993003
start: 2661
end: 2663
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2329470
display id: annotation2329470
version: 1
top level: BBa_K993003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329470/range2329470/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329470/range2329470/1
type: Range
persistent identity: range2329470
display id: range2329470
version: 1
direction: direction/forward
top level: BBa_K993003
start: 2320
end: 2322
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2329461
display id: annotation2329461
version: 1
top level: BBa_K993003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329461/range2329461/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329461/range2329461/1
type: Range
persistent identity: range2329461
display id: range2329461
version: 1
direction: direction/forward
top level: BBa_K993003
start: 1919
end: 1941
orientation: inline

Urease B
type: SequenceAnnotation
persistent identity: annotation2329463
display id: annotation2329463
version: 1
top level: BBa_K993003
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329463/range2329463/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329463/range2329463/1
type: Range
persistent identity: range2329463
display id: range2329463
version: 1
direction: direction/forward
top level: BBa_K993003
start: 1942
end: 2322
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation2329462
display id: annotation2329462
version: 1
top level: BBa_K993003
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329462/range2329462/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329462/range2329462/1
type: Range
persistent identity: range2329462
display id: range2329462
version: 1
direction: direction/forward
top level: BBa_K993003
start: 194
end: 196
orientation: inline

ROSE Thermometer
type: SequenceAnnotation
persistent identity: annotation2329458
display id: annotation2329458
version: 1
top level: BBa_K993003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329458/range2329458/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329458/range2329458/1
type: Range
persistent identity: range2329458
display id: range2329458
version: 1
direction: direction/forward
top level: BBa_K993003
start: 98
end: 193
orientation: inline

Insulator
type: SequenceAnnotation
persistent identity: annotation2329460
display id: annotation2329460
version: 1
top level: BBa_K993003
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329460/range2329460/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329460/range2329460/1
type: Range
persistent identity: range2329460
display id: range2329460
version: 1
direction: direction/forward
top level: BBa_K993003
start: 1904
end: 1918
orientation: inline

Urease C
type: SequenceAnnotation
persistent identity: annotation2329466
display id: annotation2329466
version: 1
top level: BBa_K993003
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329466/range2329466/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329466/range2329466/1
type: Range
persistent identity: range2329466
display id: range2329466
version: 1
direction: direction/forward
top level: BBa_K993003
start: 2361
end: 2663
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation2329468
display id: annotation2329468
version: 1
top level: BBa_K993003
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329468/range2329468/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329468/range2329468/1
type: Range
persistent identity: range2329468
display id: range2329468
version: 1
direction: direction/forward
top level: BBa_K993003
start: 2361
end: 2363
orientation: inline

Insulator
type: SequenceAnnotation
persistent identity: annotation2329464
display id: annotation2329464
version: 1
top level: BBa_K993003
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329464/range2329464/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329464/range2329464/1
type: Range
persistent identity: range2329464
display id: range2329464
version: 1
direction: direction/forward
top level: BBa_K993003
start: 2323
end: 2337
orientation: inline

Urease A
type: SequenceAnnotation
persistent identity: annotation2329459
display id: annotation2329459
version: 1
top level: BBa_K993003
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329459/range2329459/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329459/range2329459/1
type: Range
persistent identity: range2329459
display id: range2329459
version: 1
direction: direction/forward
top level: BBa_K993003
start: 194
end: 1903
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2329465
display id: annotation2329465
version: 1
top level: BBa_K993003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329465/range2329465/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329465/range2329465/1
type: Range
persistent identity: range2329465
display id: range2329465
version: 1
direction: direction/forward
top level: BBa_K993003
start: 2338
end: 2360
orientation: inline

BBa_K1486015
type: ComponentDefinition
persistent identity: BBa K1486015
display id: BBa_K1486015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IFP[2]-CpxR under pBAD
creator: EPFL iGem team 2014
created: 2014-09-09T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has a fused protein downstream of an arabinose-inducible promoter: IFP[2]-CpxR. Biobrick BBa_K1486014 has IFP[1]-CpxR with an arabinose promoter. This construct was made in both orientations: BBa_K1486013 is CpxR-IFP[2] (with an arabinose promoter).
mutable notes: Both orientations were made, as well as for IFP[1] so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used to fuse both proteins, and an RBS was put before each one of them.
mutable provenance: To edit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486004
component: BBa_I0500
component: BBa_K1486000
component: BBa_B0034
component: BBa_K1486007
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486000
sequence annotation: BBa_K1486004
sequence annotation: BBa_K1486007
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1486015_sequence/1

BBa_K1486004
type: Component
persistent identity: component2382855
display id: component2382855
version: 1
top level: BBa_K1486015
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_I0500
type: Component
persistent identity: component2382848
display id: component2382848
version: 1
top level: BBa_K1486015
access: public
definition: pBad/araC

BBa_K1486000
type: Component
persistent identity: component2382859
display id: component2382859
version: 1
top level: BBa_K1486015
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_B0034
type: Component
persistent identity: component2382850
display id: component2382850
version: 1
top level: BBa_K1486015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486007
type: Component
persistent identity: component2382854
display id: component2382854
version: 1
top level: BBa_K1486015
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486007/1

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2382848
display id: annotation2382848
version: 1
top level: BBa_K1486015
location: https://synbiohub.org/public/igem/BBa_K1486015/annotation2382848/range2382848/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486015/annotation2382848/range2382848/1
type: Range
persistent identity: range2382848
display id: range2382848
version: 1
direction: direction/reverse
top level: BBa_K1486015
start: 1
end: 1210
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2382859
display id: annotation2382859
version: 1
top level: BBa_K1486015
location: https://synbiohub.org/public/igem/BBa_K1486015/annotation2382859/range2382859/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486015/annotation2382859/range2382859/1
type: Range
persistent identity: range2382859
display id: range2382859
version: 1
direction: direction/reverse
top level: BBa_K1486015
start: 1860
end: 2561
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2382855
display id: annotation2382855
version: 1
top level: BBa_K1486015
location: https://synbiohub.org/public/igem/BBa_K1486015/annotation2382855/range2382855/1
component: BBa_K1486004

https://synbiohub.org/public/igem/BBa_K1486015/annotation2382855/range2382855/1
type: Range
persistent identity: range2382855
display id: range2382855
version: 1
direction: direction/reverse
top level: BBa_K1486015
start: 1824
end: 1853
orientation: inline

BBa_K1486007
type: SequenceAnnotation
persistent identity: annotation2382854
display id: annotation2382854
version: 1
top level: BBa_K1486015
location: https://synbiohub.org/public/igem/BBa_K1486015/annotation2382854/range2382854/1
component: BBa_K1486007

https://synbiohub.org/public/igem/BBa_K1486015/annotation2382854/range2382854/1
type: Range
persistent identity: range2382854
display id: range2382854
version: 1
direction: direction/reverse
top level: BBa_K1486015
start: 1237
end: 1815
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2382850
display id: annotation2382850
version: 1
top level: BBa_K1486015
location: https://synbiohub.org/public/igem/BBa_K1486015/annotation2382850/range2382850/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486015/annotation2382850/range2382850/1
type: Range
persistent identity: range2382850
display id: range2382850
version: 1
direction: direction/reverse
top level: BBa_K1486015
start: 1219
end: 1230
orientation: inline

BBa_K1486055
type: ComponentDefinition
persistent identity: BBa K1486055
display id: BBa_K1486055
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CheY/CheZ + split fLuc
creator: EPFL iGem team 2014
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: to edit
mutable notes: to edit
mutable provenance: to edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_B0034
component: BBa_K1486053
component: BBa_K629003
component: BBa_K1486017
component: BBa_I0500
component: BBa_K569017
component: BBa_K1486016
sequence annotation: BBa_K569017
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486017
sequence annotation: BBa_K1486053
sequence annotation: BBa_K1486016
sequence annotation: BBa_I0500
sequence annotation: BBa_K629003
sequence: https://synbiohub.org/public/igem/BBa_K1486055_sequence/1

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type: Component
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top level: BBa_K1486055
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definition: https://synbiohub.org/public/igem/BBa_K629003/1

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definition: https://synbiohub.org/public/igem/BBa_K1486017/1

BBa_I0500
type: Component
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definition: pBad/araC

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top level: BBa_K1486055
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definition: https://synbiohub.org/public/igem/BBa_B0034/1

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top level: BBa_K1486055
access: public
definition: https://synbiohub.org/public/igem/BBa_K569017/1

BBa_K1486053
type: Component
persistent identity: component2399883
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top level: BBa_K1486055
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definition: https://synbiohub.org/public/igem/BBa_K1486053/1

BBa_K1486016
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display id: component2399884
version: 1
top level: BBa_K1486055
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486016/1

BBa_K569017
type: SequenceAnnotation
persistent identity: annotation2399882
display id: annotation2399882
version: 1
top level: BBa_K1486055
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component: BBa_K569017

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persistent identity: range2399882
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BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2399886
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top level: BBa_K1486055
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component: BBa_B0034

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type: Range
persistent identity: range2399886
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BBa_K1486017
type: SequenceAnnotation
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BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2399881
display id: annotation2399881
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component: BBa_B0034

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BBa_K1486053
type: SequenceAnnotation
persistent identity: annotation2399894
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top level: BBa_K1486055
location: https://synbiohub.org/public/igem/BBa_K1486055/annotation2399894/range2399894/1
component: BBa_K1486053

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persistent identity: range2399894
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BBa_K1486016
type: SequenceAnnotation
persistent identity: annotation2399884
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top level: BBa_K1486055
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component: BBa_K1486016

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type: Range
persistent identity: range2399884
display id: range2399884
version: 1
direction: direction/reverse
top level: BBa_K1486055
start: 1671
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BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2399879
display id: annotation2399879
version: 1
top level: BBa_K1486055
location: https://synbiohub.org/public/igem/BBa_K1486055/annotation2399879/range2399879/1
component: BBa_I0500

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type: Range
persistent identity: range2399879
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direction: direction/reverse
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type: SequenceAnnotation
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component: BBa_K1486053

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type: Range
persistent identity: range2399883
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BBa_K629003
type: SequenceAnnotation
persistent identity: annotation2399893
display id: annotation2399893
version: 1
top level: BBa_K1486055
location: https://synbiohub.org/public/igem/BBa_K1486055/annotation2399893/range2399893/1
component: BBa_K629003

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type: Range
persistent identity: range2399893
display id: range2399893
version: 1
direction: direction/reverse
top level: BBa_K1486055
start: 2157
end: 2801
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BBa_K1486002
type: ComponentDefinition
persistent identity: BBa K1486002
display id: BBa_K1486002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Arabinose promoter + sfGFP CpxR (Nterm)
creator: iGem EPFL 2014
created: 2014-08-11T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is composed of an arabinose-inducible promoter for superfolded GFP attached by a linker to CpxR (on the N terminus of CpxR).
mutable notes: We had to test both attachments, N and C terminus of the CpxR.
mutable provenance: The arabinose promoter and the sfGFP were obtained from the registry, while the CpxR was obtained from the E coli genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_I746916
component: BBa_K1486003
component: BBa_B0034
component: BBa_K1486000
component: BBa_I0500
sequence annotation: BBa_K1486003
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486000
sequence annotation: BBa_I746916
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1486002_sequence/1

BBa_I746916
type: Component
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definition: https://synbiohub.org/public/igem/BBa_I746916/1

BBa_K1486003
type: Component
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definition: https://synbiohub.org/public/igem/BBa_K1486003/1

BBa_B0034
type: Component
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display id: component2382059
version: 1
top level: BBa_K1486002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486000
type: Component
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display id: component2382068
version: 1
top level: BBa_K1486002
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definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_I0500
type: Component
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display id: component2382057
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access: public
definition: pBad/araC

BBa_K1486003
type: SequenceAnnotation
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top level: BBa_K1486002
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type: Range
persistent identity: range2382064
display id: range2382064
version: 1
direction: direction/reverse
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orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2382057
display id: annotation2382057
version: 1
top level: BBa_K1486002
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type: Range
persistent identity: range2382057
display id: range2382057
version: 1
direction: direction/reverse
top level: BBa_K1486002
start: 1
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orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2382068
display id: annotation2382068
version: 1
top level: BBa_K1486002
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component: BBa_K1486000

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type: Range
persistent identity: range2382068
display id: range2382068
version: 1
direction: direction/reverse
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end: 2668
orientation: inline

BBa_I746916
type: SequenceAnnotation
persistent identity: annotation2382063
display id: annotation2382063
version: 1
top level: BBa_K1486002
location: https://synbiohub.org/public/igem/BBa_K1486002/annotation2382063/range2382063/1
component: BBa_I746916

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type: Range
persistent identity: range2382063
display id: range2382063
version: 1
direction: direction/reverse
top level: BBa_K1486002
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orientation: inline

BBa_B0034
type: SequenceAnnotation
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version: 1
top level: BBa_K1486002
location: https://synbiohub.org/public/igem/BBa_K1486002/annotation2382059/range2382059/1
component: BBa_B0034

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type: Range
persistent identity: range2382059
display id: range2382059
version: 1
direction: direction/reverse
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orientation: inline

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type: ComponentDefinition
persistent identity: BBa K1486005
display id: BBa_K1486005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Arabinose promoter + sfGFP-CpxR [Cterm]
creator: iGem EPFL 2014
created: 2014-08-12T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is composed of an arabinose-inducible promoter for superfolded GFP attached by a linker to CpxR (on the C terminus of CpxR).
mutable notes: We had to test both attachments, N and C terminus of the CpxR.
mutable provenance: The arabinose promoter and the sfGFP were obtained from the registry, while the CpxR was obtained from the E coli genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
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component: BBa_K1486000
component: BBa_I0500
sequence annotation: BBa_K1486003
sequence annotation: BBa_I746916
sequence annotation: BBa_B0034
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486000
sequence: https://synbiohub.org/public/igem/BBa_K1486005_sequence/1

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type: Component
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definition: https://synbiohub.org/public/igem/BBa_I746916/1

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display id: component2382084
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486000
type: Component
persistent identity: component2382088
display id: component2382088
version: 1
top level: BBa_K1486005
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_I0500
type: Component
persistent identity: component2382082
display id: component2382082
version: 1
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access: public
definition: pBad/araC

BBa_K1486003
type: SequenceAnnotation
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version: 1
top level: BBa_K1486005
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type: Range
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orientation: inline

BBa_I746916
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component: BBa_I746916

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type: Range
persistent identity: range2382093
display id: range2382093
version: 1
direction: direction/reverse
top level: BBa_K1486005
start: 1949
end: 2668
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2382084
display id: annotation2382084
version: 1
top level: BBa_K1486005
location: https://synbiohub.org/public/igem/BBa_K1486005/annotation2382084/range2382084/1
component: BBa_B0034

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type: Range
persistent identity: range2382084
display id: range2382084
version: 1
direction: direction/reverse
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BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2382082
display id: annotation2382082
version: 1
top level: BBa_K1486005
location: https://synbiohub.org/public/igem/BBa_K1486005/annotation2382082/range2382082/1
component: BBa_I0500

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type: Range
persistent identity: range2382082
display id: range2382082
version: 1
direction: direction/reverse
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start: 1
end: 1210
orientation: inline

BBa_K1486000
type: SequenceAnnotation
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display id: annotation2382088
version: 1
top level: BBa_K1486005
location: https://synbiohub.org/public/igem/BBa_K1486005/annotation2382088/range2382088/1
component: BBa_K1486000

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type: Range
persistent identity: range2382088
display id: range2382088
version: 1
direction: direction/reverse
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BBa_K1486040
type: ComponentDefinition
persistent identity: BBa K1486040
display id: BBa_K1486040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFPN + CpxR
creator: EPFL iGem team 2014
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486037
component: BBa_K1486000
component: BBa_B0034
component: BBa_I0500
component: BBa_K1486038
sequence annotation: BBa_K1486038
sequence annotation: BBa_K1486000
sequence annotation: BBa_K1486037
sequence annotation: BBa_B0034
sequence annotation: BBa_I0500
sequence: https://synbiohub.org/public/igem/BBa_K1486040_sequence/1

BBa_K1486037
type: Component
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display id: component2387189
version: 1
top level: BBa_K1486040
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486037/1

BBa_K1486000
type: Component
persistent identity: component2387193
display id: component2387193
version: 1
top level: BBa_K1486040
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_B0034
type: Component
persistent identity: component2387187
display id: component2387187
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I0500
type: Component
persistent identity: component2387185
display id: component2387185
version: 1
top level: BBa_K1486040
access: public
definition: pBad/araC

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type: Component
persistent identity: component2387188
display id: component2387188
version: 1
top level: BBa_K1486040
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486038/1

BBa_K1486038
type: SequenceAnnotation
persistent identity: annotation2387188
display id: annotation2387188
version: 1
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display id: range2387188
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type: SequenceAnnotation
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type: Range
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BBa_K1486037
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display id: annotation2387189
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top level: BBa_K1486040
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component: BBa_K1486037

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type: Range
persistent identity: range2387189
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BBa_B0034
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display id: annotation2387187
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component: BBa_B0034

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persistent identity: range2387187
display id: range2387187
version: 1
direction: direction/reverse
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BBa_I0500
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persistent identity: range2387185
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direction: direction/reverse
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orientation: inline

BBa_K1486008
type: ComponentDefinition
persistent identity: BBa K1486008
display id: BBa_K1486008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CxpR & Split IFP1.4 [Cterm + Cterm][1]
creator: iGem EPFL 2014
created: 2014-08-25T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has two fused proteins downstream of an arabinose-inducible promoter: CpxR-IFP[1] and CpxR-IFP[2]. This construct was made in all orientations: - IFP[1]-CpxR + IFP[2]-CpxR (BBa_K1286009) - IFP[1]-CpxR + CpxR-IFP[2] (BBa_K1486010) - CpxR-IFP[1] + IFP[2]-CpxR (BBa_K1486011)
mutable notes: All orientations were made, so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used for each of the fused proteins, and an RBS was put before each one of them.
mutable provenance: From Michnick Article. To Edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486006
component: BBa_B0034
component: BBa_I0500
component: BBa_K1486000
component: BBa_K1486004
component: BBa_K1486007
component: BBa_K1486052
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486052
sequence annotation: BBa_K1486000
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486004
sequence annotation: BBa_K1486006
sequence annotation: BBa_K1486007
sequence: https://synbiohub.org/public/igem/BBa_K1486008_sequence/1

BBa_K1486006
type: Component
persistent identity: component2418045
display id: component2418045
version: 1
top level: BBa_K1486008
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486006/1

BBa_B0034
type: Component
persistent identity: component2418048
display id: component2418048
version: 1
top level: BBa_K1486008
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I0500
type: Component
persistent identity: component2418034
display id: component2418034
version: 1
top level: BBa_K1486008
access: public
definition: pBad/araC

BBa_K1486000
type: Component
persistent identity: component2418040
display id: component2418040
version: 1
top level: BBa_K1486008
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_K1486000
type: Component
persistent identity: component2418052
display id: component2418052
version: 1
top level: BBa_K1486008
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_K1486004
type: Component
persistent identity: component2418053
display id: component2418053
version: 1
top level: BBa_K1486008
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_B0034
type: Component
persistent identity: component2418036
display id: component2418036
version: 1
top level: BBa_K1486008
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486007
type: Component
persistent identity: component2418057
display id: component2418057
version: 1
top level: BBa_K1486008
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486007/1

BBa_K1486004
type: Component
persistent identity: component2418041
display id: component2418041
version: 1
top level: BBa_K1486008
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486052
type: Component
persistent identity: component2418046
display id: component2418046
version: 1
top level: BBa_K1486008
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486052/1

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2418034
display id: annotation2418034
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418034/range2418034/1
component: BBa_I0500

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type: Range
persistent identity: range2418034
display id: range2418034
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 1
end: 1210
orientation: inline

BBa_K1486052
type: SequenceAnnotation
persistent identity: annotation2418046
display id: annotation2418046
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418046/range2418046/1
component: BBa_K1486052

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type: Range
persistent identity: range2418046
display id: range2418046
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 2360
end: 2397
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2418040
display id: annotation2418040
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418040/range2418040/1
component: BBa_K1486000

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type: Range
persistent identity: range2418040
display id: range2418040
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 1223
end: 1924
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2418052
display id: annotation2418052
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418052/range2418052/1
component: BBa_K1486000

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type: Range
persistent identity: range2418052
display id: range2418052
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 2410
end: 3111
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2418036
display id: annotation2418036
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418036/range2418036/1
component: BBa_B0034

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type: Range
persistent identity: range2418036
display id: range2418036
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 1211
end: 1222
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2418041
display id: annotation2418041
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418041/range2418041/1
component: BBa_K1486004

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type: Range
persistent identity: range2418041
display id: range2418041
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 1925
end: 1954
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2418048
display id: annotation2418048
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418048/range2418048/1
component: BBa_B0034

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type: Range
persistent identity: range2418048
display id: range2418048
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 2398
end: 2409
orientation: inline

BBa_K1486006
type: SequenceAnnotation
persistent identity: annotation2418045
display id: annotation2418045
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418045/range2418045/1
component: BBa_K1486006

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type: Range
persistent identity: range2418045
display id: range2418045
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 1955
end: 2359
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2418053
display id: annotation2418053
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418053/range2418053/1
component: BBa_K1486004

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type: Range
persistent identity: range2418053
display id: range2418053
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 3112
end: 3141
orientation: inline

BBa_K1486007
type: SequenceAnnotation
persistent identity: annotation2418057
display id: annotation2418057
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418057/range2418057/1
component: BBa_K1486007

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type: Range
persistent identity: range2418057
display id: range2418057
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 3142
end: 3726
orientation: inline

BBa_K1486001
type: ComponentDefinition
persistent identity: BBa K1486001
display id: BBa_K1486001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Arabinose promoter + CpxR
creator: iGem EPFL 2014
created: 2014-08-11T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The part contains CpxR with an upstream arabinose-inducible promoter.
mutable notes: The arabinose had to be upstream of the CpxR. Also, the sequence had to be tested because of a mutation, and it works perfectly.
mutable provenance: The CpxR comes from the genome of E coli, and the arabinose promoter comes from a biobrick.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_I0500
component: BBa_K1486000
component: BBa_B0034
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486000
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1486001_sequence/1

BBa_I0500
type: Component
persistent identity: component2382072
display id: component2382072
version: 1
top level: BBa_K1486001
access: public
definition: pBad/araC

BBa_K1486000
type: Component
persistent identity: component2382078
display id: component2382078
version: 1
top level: BBa_K1486001
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_B0034
type: Component
persistent identity: component2382074
display id: component2382074
version: 1
top level: BBa_K1486001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2382072
display id: annotation2382072
version: 1
top level: BBa_K1486001
location: https://synbiohub.org/public/igem/BBa_K1486001/annotation2382072/range2382072/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486001/annotation2382072/range2382072/1
type: Range
persistent identity: range2382072
display id: range2382072
version: 1
direction: direction/reverse
top level: BBa_K1486001
start: 1
end: 1210
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2382078
display id: annotation2382078
version: 1
top level: BBa_K1486001
location: https://synbiohub.org/public/igem/BBa_K1486001/annotation2382078/range2382078/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486001/annotation2382078/range2382078/1
type: Range
persistent identity: range2382078
display id: range2382078
version: 1
direction: direction/reverse
top level: BBa_K1486001
start: 1223
end: 1924
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2382074
display id: annotation2382074
version: 1
top level: BBa_K1486001
location: https://synbiohub.org/public/igem/BBa_K1486001/annotation2382074/range2382074/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486001/annotation2382074/range2382074/1
type: Range
persistent identity: range2382074
display id: range2382074
version: 1
direction: direction/reverse
top level: BBa_K1486001
start: 1211
end: 1222
orientation: inline

BBa_K332033
type: ComponentDefinition
persistent identity: BBa K332033
display id: BBa_K332033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 37℃ induced RBS + tetR + double terminator
creator: KUO, CHIA-CHUN
created: 2010-10-23T11:00:00Z
modified: 2015-05-08T01:12:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _452_
m user id: 0
owner id: 7059
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: It is a transcriptional unit including 37℃ induced RBS + tetR + double terminator. It can be added various promoter to adapt your conditions.
mutable notes: To regulate following genes by temperature. To maintain cells at 37?? C ↑ : the tetR protein be generated and repress Ptet's circuit. To release cells at 37?? C ↓ : the tetR protein not be generated and do not repress Ptet's circuit.
mutable provenance: the 37℃ induced RBS cames from iGEM08_TUDelft
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K115002
component: BBa_C0040
component: BBa_J61048
sequence annotation: BBa_K115002
sequence annotation: BBa_J61048
sequence annotation: BBa_C0040
sequence: https://synbiohub.org/public/igem/BBa_K332033_sequence/1

BBa_K115002
type: Component
persistent identity: component2247611
display id: component2247611
version: 1
top level: BBa_K332033
access: public
definition: https://synbiohub.org/public/igem/BBa_K115002/1

BBa_C0040
type: Component
persistent identity: component2247615
display id: component2247615
version: 1
top level: BBa_K332033
access: public
definition: https://synbiohub.org/public/igem/BBa_C0040/1

BBa_J61048
type: Component
persistent identity: component2247616
display id: component2247616
version: 1
top level: BBa_K332033
access: public
definition: https://synbiohub.org/public/igem/BBa_J61048/1

BBa_K115002
type: SequenceAnnotation
persistent identity: annotation2247611
display id: annotation2247611
version: 1
top level: BBa_K332033
location: https://synbiohub.org/public/igem/BBa_K332033/annotation2247611/range2247611/1
component: BBa_K115002

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type: Range
persistent identity: range2247611
display id: range2247611
version: 1
direction: direction/reverse
top level: BBa_K332033
start: 1
end: 52
orientation: inline

BBa_J61048
type: SequenceAnnotation
persistent identity: annotation2247616
display id: annotation2247616
version: 1
top level: BBa_K332033
location: https://synbiohub.org/public/igem/BBa_K332033/annotation2247616/range2247616/1
component: BBa_J61048

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type: Range
persistent identity: range2247616
display id: range2247616
version: 1
direction: direction/reverse
top level: BBa_K332033
start: 752
end: 864
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2247615
display id: annotation2247615
version: 1
top level: BBa_K332033
location: https://synbiohub.org/public/igem/BBa_K332033/annotation2247615/range2247615/1
component: BBa_C0040

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type: Range
persistent identity: range2247615
display id: range2247615
version: 1
direction: direction/reverse
top level: BBa_K332033
start: 59
end: 743
orientation: inline

BBa_K772009
type: ComponentDefinition
persistent identity: BBa K772009
display id: BBa_K772009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP - LasR protein complex with flag tags
creator: Mustafa Elitok
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
group u list: _1024_
m user id: 0
owner id: 9573
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part contains a green fluorescent protein (GFP) as a non-reactive chemical compound (anchor) in order to neutralize the active reactive fixed to it, an inducer regulatory protein (LasR is responsible to induce pLas promoter in the presence of 6HSLO3 which is a chemical compound sythesized by an enzyme that LasI gene codes.) and a TEV protease cleavage site between them to assemble these two proteins. BBa_772001 and BBa_K772002 are assembled to be used as the trigger of detection in the whole system which ends with the intracellular transfection (or activation) of TEV protease. After the activation, it is planned that TEV protease will cleave this anchor-inducer protein complex to release free the reporter. In conclusion, in this system, the inducer would activate a transcription pathway on the targeted inducible gene or the reporter would indicate that there is a change in the whole system. This part contains flag tags extra from BBa_K772006 in the GFP region; because it is planned to be used in the Co-IP/SDS-PAGE assay to be isolated.
mutable notes: Note that there is an RBS sequence just before the protein sequence in order to eliminate the need of addition of it.
mutable provenance: Cleavage site, LasR and GFP sequences are gathered from Registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
sequence annotation: Flag tag
sequence annotation: RBS
sequence annotation: GFP
sequence annotation: Double terminator
sequence annotation: LasR
sequence annotation: Anchor-inducer + flag
sequence annotation: TEV cleavage site
sequence: https://synbiohub.org/public/igem/BBa_K772009_sequence/1

Flag tag
type: SequenceAnnotation
persistent identity: annotation2203918
display id: annotation2203918
version: 1
top level: BBa_K772009
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K772009/annotation2203918/range2203918/1

https://synbiohub.org/public/igem/BBa_K772009/annotation2203918/range2203918/1
type: Range
persistent identity: range2203918
display id: range2203918
version: 1
direction: direction/forward
top level: BBa_K772009
start: 21
end: 46
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2203917
display id: annotation2203917
version: 1
top level: BBa_K772009
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K772009/annotation2203917/range2203917/1

https://synbiohub.org/public/igem/BBa_K772009/annotation2203917/range2203917/1
type: Range
persistent identity: range2203917
display id: range2203917
version: 1
direction: direction/forward
top level: BBa_K772009
start: 1
end: 20
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation2203919
display id: annotation2203919
version: 1
top level: BBa_K772009
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772009/annotation2203919/range2203919/1

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type: Range
persistent identity: range2203919
display id: range2203919
version: 1
direction: direction/forward
top level: BBa_K772009
start: 47
end: 767
orientation: inline

Double terminator
type: SequenceAnnotation
persistent identity: annotation2203922
display id: annotation2203922
version: 1
top level: BBa_K772009
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K772009/annotation2203922/range2203922/1

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type: Range
persistent identity: range2203922
display id: range2203922
version: 1
direction: direction/forward
top level: BBa_K772009
start: 1615
end: 1745
orientation: inline

LasR
type: SequenceAnnotation
persistent identity: annotation2203921
display id: annotation2203921
version: 1
top level: BBa_K772009
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772009/annotation2203921/range2203921/1

https://synbiohub.org/public/igem/BBa_K772009/annotation2203921/range2203921/1
type: Range
persistent identity: range2203921
display id: range2203921
version: 1
direction: direction/forward
top level: BBa_K772009
start: 858
end: 1614
orientation: inline

Anchor-inducer + flag
type: SequenceAnnotation
persistent identity: annotation2203923
display id: annotation2203923
version: 1
top level: BBa_K772009
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K772009/annotation2203923/range2203923/1

https://synbiohub.org/public/igem/BBa_K772009/annotation2203923/range2203923/1
type: Range
persistent identity: range2203923
display id: range2203923
version: 1
direction: direction/forward
top level: BBa_K772009
start: 21
end: 1614
orientation: inline

TEV cleavage site
type: SequenceAnnotation
persistent identity: annotation2203920
display id: annotation2203920
version: 1
top level: BBa_K772009
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K772009/annotation2203920/range2203920/1

https://synbiohub.org/public/igem/BBa_K772009/annotation2203920/range2203920/1
type: Range
persistent identity: range2203920
display id: range2203920
version: 1
direction: direction/forward
top level: BBa_K772009
start: 768
end: 857
orientation: inline

BBa_K993002
type: ComponentDefinition
persistent identity: BBa K993002
display id: BBa_K993002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IbpB RNA Thermometer with RFP
creator: İBRAHİM YASİR ORHAN
created: 2013-06-13T11:00:00Z
modified: 2015-05-08T01:13:51Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1290_
m user id: 0
owner id: 11153
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: The IbpB thermometer is an RNA thermometer element found in the ibpAB operon. The operon contains two heat-shock genes, encoding inclusion body binding proteins A and B (IbpA/B), and is the most drastically upregulated operon under heat-shock in Escherichia coli. IbpA is regulated by a ROSE element found in its 5' UTR, while IbpB has its own heat-sensitive cis-regulatory element. The activity of this thermoregulator was confirmed in vitro but was not found in vivo, suggesting more complicated operon regulation exists in bacterial cells. In this part, red fluorescent protein (RFP) is added in the downstream region to measure the activity of RNA thermometer. You check our Experience page for further information about our characterization and experiments. For the inner elements in the part design; check out the Part Design page. RNA Thermometers are the only known single-component regulators of gene expression.(1) RNA Thermometers are thermosensors that regulate gene expression by temperature-induced changes in RNA conformation. Naturally occurring RNA Thermometers exhibit complex secondary structures which are believed to undergo a series of gradual structural changes in response to temperature shifts.(2) RNA Thermometers often regulate genes required during either a heat shock or cold shock response, but have been implicated in other regulatory roles such as in pathogenecity and starvation.(3)
mutable notes: Note that RNA thermometer actually works as an RBS sequence. Therefore, this part does not include an additional ribosome binding site. Also, this part does not contain an terminator sequence, but a simple stop codon. The RFP should be translated continuously.
mutable provenance: Escherichia coli, NCBI GeneBank Other elements are obtained from Registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: pVeg2
sequence annotation: Stop codon
sequence annotation: IbpB thermometer
sequence annotation: RFP
sequence annotation: Start codon
sequence: https://synbiohub.org/public/igem/BBa_K993002_sequence/1

pVeg2
type: SequenceAnnotation
persistent identity: annotation2329442
display id: annotation2329442
version: 1
top level: BBa_K993002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K993002/annotation2329442/range2329442/1

https://synbiohub.org/public/igem/BBa_K993002/annotation2329442/range2329442/1
type: Range
persistent identity: range2329442
display id: range2329442
version: 1
direction: direction/forward
top level: BBa_K993002
start: 1
end: 97
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2329445
display id: annotation2329445
version: 1
top level: BBa_K993002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K993002/annotation2329445/range2329445/1

https://synbiohub.org/public/igem/BBa_K993002/annotation2329445/range2329445/1
type: Range
persistent identity: range2329445
display id: range2329445
version: 1
direction: direction/forward
top level: BBa_K993002
start: 915
end: 917
orientation: inline

IbpB thermometer
type: SequenceAnnotation
persistent identity: annotation2329443
display id: annotation2329443
version: 1
top level: BBa_K993002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K993002/annotation2329443/range2329443/1

https://synbiohub.org/public/igem/BBa_K993002/annotation2329443/range2329443/1
type: Range
persistent identity: range2329443
display id: range2329443
version: 1
direction: direction/forward
top level: BBa_K993002
start: 98
end: 208
orientation: inline

RFP
type: SequenceAnnotation
persistent identity: annotation2329444
display id: annotation2329444
version: 1
top level: BBa_K993002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K993002/annotation2329444/range2329444/1

https://synbiohub.org/public/igem/BBa_K993002/annotation2329444/range2329444/1
type: Range
persistent identity: range2329444
display id: range2329444
version: 1
direction: direction/forward
top level: BBa_K993002
start: 209
end: 917
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation2329446
display id: annotation2329446
version: 1
top level: BBa_K993002
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K993002/annotation2329446/range2329446/1

https://synbiohub.org/public/igem/BBa_K993002/annotation2329446/range2329446/1
type: Range
persistent identity: range2329446
display id: range2329446
version: 1
direction: direction/forward
top level: BBa_K993002
start: 209
end: 211
orientation: inline

BBa_K993000
type: ComponentDefinition
persistent identity: BBa K993000
display id: BBa_K993000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP Generator with ROSE thermometer
creator: ABD??LKADİR KARADAĞ
created: 2013-06-13T11:00:00Z
modified: 2015-05-08T01:13:51Z
discontinued: false
dominant: false
group u list: _1290_
m user id: 0
owner id: 11156
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The repression of heat shock gene expression (ROSE) element is an RNA element found in the 5' UTR of some heat shock protein's mRNAs. The ROSE element is an RNA thermometer that negatively regulates heat shock gene expression. The secondary structure is thought to be altered by temperature, thus it is an RNA thermometer. This structure blocks access to the ribosome binding site at normal temperatures. During heat shock however, the structure changes freeing the ribosome binding site and allowing expression to occur. RNA Thermometers are the only known single-component regulators of gene expression.(1) RNA Thermometers are thermosensors that regulate gene expression by temperature-induced changes in RNA conformation. Naturally occurring RNA Thermometers exhibit complex secondary structures which are believed to undergo a series of gradual structural changes in response to temperature shifts.(2) RNA Thermometers often regulate genes required during either a heat shock or cold shock response, but have been implicated in other regulatory roles such as in pathogenecity and starvation.(3)
mutable notes: Note that RNA thermometer actually works as an RBS sequence. Therefore, this part does not include an additional ribosome binding site. Also, this part does not contain an terminator sequence, but a simple stop codon. The RFP should be translated continuously.
mutable provenance: Bradyrhizobium japonicum USDA 110 and Registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: pVeg2
sequence annotation: RFP Generator
sequence annotation: Start Codon
sequence annotation: ROSE Thermometer
sequence annotation: Finish Codon
sequence: https://synbiohub.org/public/igem/BBa_K993000_sequence/1

pVeg2
type: SequenceAnnotation
persistent identity: annotation2329453
display id: annotation2329453
version: 1
top level: BBa_K993000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K993000/annotation2329453/range2329453/1

https://synbiohub.org/public/igem/BBa_K993000/annotation2329453/range2329453/1
type: Range
persistent identity: range2329453
display id: range2329453
version: 1
direction: direction/forward
top level: BBa_K993000
start: 1
end: 97
orientation: inline

RFP Generator
type: SequenceAnnotation
persistent identity: annotation2329455
display id: annotation2329455
version: 1
top level: BBa_K993000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K993000/annotation2329455/range2329455/1

https://synbiohub.org/public/igem/BBa_K993000/annotation2329455/range2329455/1
type: Range
persistent identity: range2329455
display id: range2329455
version: 1
direction: direction/forward
top level: BBa_K993000
start: 194
end: 902
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation2329457
display id: annotation2329457
version: 1
top level: BBa_K993000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K993000/annotation2329457/range2329457/1

https://synbiohub.org/public/igem/BBa_K993000/annotation2329457/range2329457/1
type: Range
persistent identity: range2329457
display id: range2329457
version: 1
direction: direction/forward
top level: BBa_K993000
start: 194
end: 196
orientation: inline

ROSE Thermometer
type: SequenceAnnotation
persistent identity: annotation2329454
display id: annotation2329454
version: 1
top level: BBa_K993000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K993000/annotation2329454/range2329454/1

https://synbiohub.org/public/igem/BBa_K993000/annotation2329454/range2329454/1
type: Range
persistent identity: range2329454
display id: range2329454
version: 1
direction: direction/forward
top level: BBa_K993000
start: 98
end: 193
orientation: inline

Finish Codon
type: SequenceAnnotation
persistent identity: annotation2329456
display id: annotation2329456
version: 1
top level: BBa_K993000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K993000/annotation2329456/range2329456/1

https://synbiohub.org/public/igem/BBa_K993000/annotation2329456/range2329456/1
type: Range
persistent identity: range2329456
display id: range2329456
version: 1
direction: direction/forward
top level: BBa_K993000
start: 900
end: 902
orientation: inline

BBa_K1468004
type: ComponentDefinition
persistent identity: BBa K1468004
display id: BBa_K1468004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTet + gene encoding LacZ
creator: Pedro Luis Dorado Morales
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:10:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1847_
m user id: 0
owner id: 11756
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: LacZ protein
sequence annotation: STOP
sequence annotation: BBa_R0040
sequence annotation: BBa_I732005
sequence annotation: TetR 1
sequence annotation: TetR 2
sequence annotation: LacZ
sequence annotation: -35
sequence annotation: START
sequence annotation: -10
sequence annotation: BBa_K1468004
sequence annotation: C --> T
sequence: https://synbiohub.org/public/igem/BBa_K1468004_sequence/1

LacZ protein
type: SequenceAnnotation
persistent identity: annotation2418063
display id: annotation2418063
version: 1
top level: BBa_K1468004
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2418063/range2418063/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2418063/range2418063/1
type: Range
persistent identity: range2418063
display id: range2418063
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 61
end: 3129
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation2400599
display id: annotation2400599
version: 1
top level: BBa_K1468004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400599/range2400599/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400599/range2400599/1
type: Range
persistent identity: range2400599
display id: range2400599
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 3129
end: 3129
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2400593
display id: annotation2400593
version: 1
top level: BBa_K1468004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400593/range2400593/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400593/range2400593/1
type: Range
persistent identity: range2400593
display id: range2400593
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 1
end: 54
orientation: inline

BBa_I732005
type: SequenceAnnotation
persistent identity: annotation2400600
display id: annotation2400600
version: 1
top level: BBa_K1468004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400600/range2400600/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400600/range2400600/1
type: Range
persistent identity: range2400600
display id: range2400600
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 61
end: 3129
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation2400591
display id: annotation2400591
version: 1
top level: BBa_K1468004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400591/range2400591/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400591/range2400591/1
type: Range
persistent identity: range2400591
display id: range2400591
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 1
end: 19
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation2400594
display id: annotation2400594
version: 1
top level: BBa_K1468004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400594/range2400594/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400594/range2400594/1
type: Range
persistent identity: range2400594
display id: range2400594
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 26
end: 44
orientation: inline

LacZ
type: SequenceAnnotation
persistent identity: annotation2400596
display id: annotation2400596
version: 1
top level: BBa_K1468004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400596/range2400596/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400596/range2400596/1
type: Range
persistent identity: range2400596
display id: range2400596
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 61
end: 3129
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2400592
display id: annotation2400592
version: 1
top level: BBa_K1468004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400592/range2400592/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400592/range2400592/1
type: Range
persistent identity: range2400592
display id: range2400592
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 20
end: 25
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation2400597
display id: annotation2400597
version: 1
top level: BBa_K1468004
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400597/range2400597/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400597/range2400597/1
type: Range
persistent identity: range2400597
display id: range2400597
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 61
end: 63
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2400595
display id: annotation2400595
version: 1
top level: BBa_K1468004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400595/range2400595/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400595/range2400595/1
type: Range
persistent identity: range2400595
display id: range2400595
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 43
end: 48
orientation: inline

BBa_K1468004
type: SequenceAnnotation
persistent identity: annotation2400601
display id: annotation2400601
version: 1
top level: BBa_K1468004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400601/range2400601/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400601/range2400601/1
type: Range
persistent identity: range2400601
display id: range2400601
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 1
end: 3129
orientation: inline

C --> T
type: SequenceAnnotation
persistent identity: annotation2400598
display id: annotation2400598
version: 1
top level: BBa_K1468004
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400598/range2400598/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400598/range2400598/1
type: Range
persistent identity: range2400598
display id: range2400598
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 3085
end: 3085
orientation: inline

BBa_K1486009
type: ComponentDefinition
persistent identity: BBa K1486009
display id: BBa_K1486009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CxpR & Split IFP1.4 [Nterm + Nterm]
creator: iGem EPFL 2014
created: 2014-08-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has two fused proteins downstream of an arabinose-inducible promoter: CpxR-IFP[1] and CpxR-IFP[2]. This construct was made in all orientations: - CpxR-IFP[1] + CpxR-IFP[2] (BBa_K1286008) - IFP[1]-CpxR + CpxR-IFP[2] (BBa_K1486010) - CpxR-IFP[1] + IFP[2]-CpxR (BBa_K1486011)
mutable notes: All orientations were made, so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used for each of the fused proteins, and an RBS was put before each one of them.
mutable provenance: From Michnick Article. To Edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486004
component: BBa_K1486000
component: BBa_K1486006
component: BBa_K1486007
component: BBa_I0500
component: BBa_B0034
component: BBa_K1486052
sequence annotation: BBa_K1486000
sequence annotation: BBa_K1486052
sequence annotation: BBa_K1486007
sequence annotation: BBa_K1486004
sequence annotation: BBa_K1486006
sequence annotation: BBa_B0034
sequence annotation: BBa_I0500
sequence: https://synbiohub.org/public/igem/BBa_K1486009_sequence/1

BBa_K1486004
type: Component
persistent identity: component2420772
display id: component2420772
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486000
type: Component
persistent identity: component2420788
display id: component2420788
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_K1486006
type: Component
persistent identity: component2420771
display id: component2420771
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486006/1

BBa_K1486007
type: Component
persistent identity: component2420783
display id: component2420783
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486007/1

BBa_I0500
type: Component
persistent identity: component2420763
display id: component2420763
version: 1
top level: BBa_K1486009
access: public
definition: pBad/araC

BBa_K1486000
type: Component
persistent identity: component2420776
display id: component2420776
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_K1486004
type: Component
persistent identity: component2420758
display id: component2420758
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486004
type: Component
persistent identity: component2420784
display id: component2420784
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486000
type: Component
persistent identity: component2420766
display id: component2420766
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_B0034
type: Component
persistent identity: component2420767
display id: component2420767
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component2420779
display id: component2420779
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486052
type: Component
persistent identity: component2420777
display id: component2420777
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486052/1

BBa_K1486007
type: Component
persistent identity: component2420756
display id: component2420756
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486007/1

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2420788
display id: annotation2420788
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420788/range2420788/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486009/annotation2420788/range2420788/1
type: Range
persistent identity: range2420788
display id: range2420788
version: 1
direction: direction/reverse
top level: BBa_K1486009
start: 3025
end: 3726
orientation: inline

BBa_K1486052
type: SequenceAnnotation
persistent identity: annotation2420777
display id: annotation2420777
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420777/range2420777/1
component: BBa_K1486052

https://synbiohub.org/public/igem/BBa_K1486009/annotation2420777/range2420777/1
type: Range
persistent identity: range2420777
display id: range2420777
version: 1
direction: direction/reverse
top level: BBa_K1486009
start: 2360
end: 2397
orientation: inline

BBa_K1486007
type: SequenceAnnotation
persistent identity: annotation2420783
display id: annotation2420783
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420783/range2420783/1
component: BBa_K1486007

https://synbiohub.org/public/igem/BBa_K1486009/annotation2420783/range2420783/1
type: Range
persistent identity: range2420783
display id: range2420783
version: 1
direction: direction/reverse
top level: BBa_K1486009
start: 2410
end: 2994
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2420772
display id: annotation2420772
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420772/range2420772/1
component: BBa_K1486004

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type: Range
persistent identity: range2420772
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BBa_K1486004
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BBa_K1486006
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BBa_K1486000
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BBa_B0034
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display id: annotation2420767
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BBa_K1486000
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BBa_B0034
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display id: annotation2420779
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BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2420763
display id: annotation2420763
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top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420763/range2420763/1
component: BBa_I0500

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persistent identity: range2420763
display id: range2420763
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BBa_K1486007
type: SequenceAnnotation
persistent identity: annotation2420756
display id: annotation2420756
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location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420756/range2420756/1
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BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2420758
display id: annotation2420758
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420758/range2420758/1
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persistent identity: range2420758
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Ara
type: ComponentDefinition
persistent identity: BBa K808000
display id: BBa_K808000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: araC-Pbad - Arabinose inducible regulatory promoter/repressor unit
creator: Valentina Herbring, Sebastian Palluk, Andreas Schmidt
created: 2012-08-10T11:00:00Z
modified: 2015-05-08T01:13:25Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1065_
m user id: 0
owner id: 11792
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This promoter can be induced by arabinose. In presens of arabinose the promotor is active.
mutable notes: non
mutable provenance: Guzman LM, Belin D, Carson MJ, Beckwith J. Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J Bacteriol. 1995 Jul;177(14):4121-30 Khlebnikov A, Skaug T, Keasling JD, modulation of gene expression from the arabinose-inducible araBAD promoter,J Ind Microbiol Biotechnol. 2002 Jul;29(1):34-7.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: AraO
sequence annotation: AraPromoter
sequence annotation: AraC
sequence annotation: AraO1
sequence: https://synbiohub.org/public/igem/BBa_K808000_sequence/1

AraO
type: SequenceAnnotation
persistent identity: annotation2200758
display id: annotation2200758
version: 1
top level: Ara
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K808000/annotation2200758/range2200758/1

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persistent identity: range2200758
display id: range2200758
version: 1
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top level: Ara
start: 909
end: 924
orientation: inline

AraPromoter
type: SequenceAnnotation
persistent identity: annotation2200760
display id: annotation2200760
version: 1
top level: Ara
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K808000/annotation2200760/range2200760/1

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type: Range
persistent identity: range2200760
display id: range2200760
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start: 1153
end: 1181
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AraC
type: SequenceAnnotation
persistent identity: annotation2200757
display id: annotation2200757
version: 1
top level: Ara
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K808000/annotation2200757/range2200757/1

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persistent identity: range2200757
display id: range2200757
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AraO1
type: SequenceAnnotation
persistent identity: annotation2200759
display id: annotation2200759
version: 1
top level: Ara
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K808000/annotation2200759/range2200759/1

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type: Range
persistent identity: range2200759
display id: range2200759
version: 1
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top level: Ara
start: 1066
end: 1077
orientation: inline

BBa_K1486010
type: ComponentDefinition
persistent identity: BBa K1486010
display id: BBa_K1486010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CxpR & Split IFP1.4 [Nterm + Cterm]
creator: iGem EPFL 2014
created: 2014-08-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has two fused proteins downstream of an arabinose-inducible promoter: CpxR-IFP[1] and CpxR-IFP[2]. This construct was made in all orientations: - CpxR-IFP[1] + CpxR-IFP[2] (BBa_K1286008) - IFP[1]-CpxR + CpxR-IFP[2] (BBa_K1486010) - CpxR-IFP[1] + IFP[2]-CpxR (BBa_K1486011)
mutable notes: All orientations were made, so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used for each of the fused proteins, and an RBS was put before each one of them.
mutable provenance: From Michnick Article. To Edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_K1486004
component: BBa_K1486007
component: BBa_K1486006
component: BBa_K1486000
component: BBa_I0500
sequence annotation: BBa_K1486004
sequence annotation: BBa_K1486000
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486006
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486007
sequence: https://synbiohub.org/public/igem/BBa_K1486010_sequence/1

BBa_B0034
type: Component
persistent identity: component2424986
display id: component2424986
version: 1
top level: BBa_K1486010
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486004
type: Component
persistent identity: component2425002
display id: component2425002
version: 1
top level: BBa_K1486010
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486004
type: Component
persistent identity: component2424991
display id: component2424991
version: 1
top level: BBa_K1486010
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486007
type: Component
persistent identity: component2425006
display id: component2425006
version: 1
top level: BBa_K1486010
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486007/1

BBa_K1486006
type: Component
persistent identity: component2424990
display id: component2424990
version: 1
top level: BBa_K1486010
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486006/1

BBa_K1486000
type: Component
persistent identity: component2425001
display id: component2425001
version: 1
top level: BBa_K1486010
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_K1486000
type: Component
persistent identity: component2424995
display id: component2424995
version: 1
top level: BBa_K1486010
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_B0034
type: Component
persistent identity: component2424997
display id: component2424997
version: 1
top level: BBa_K1486010
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I0500
type: Component
persistent identity: component2424984
display id: component2424984
version: 1
top level: BBa_K1486010
access: public
definition: pBad/araC

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2424991
display id: annotation2424991
version: 1
top level: BBa_K1486010
location: https://synbiohub.org/public/igem/BBa_K1486010/annotation2424991/range2424991/1
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type: Range
persistent identity: range2424991
display id: range2424991
version: 1
direction: direction/reverse
top level: BBa_K1486010
start: 1628
end: 1657
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2425002
display id: annotation2425002
version: 1
top level: BBa_K1486010
location: https://synbiohub.org/public/igem/BBa_K1486010/annotation2425002/range2425002/1
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persistent identity: range2425002
display id: range2425002
version: 1
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top level: BBa_K1486010
start: 3074
end: 3103
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BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2425001
display id: annotation2425001
version: 1
top level: BBa_K1486010
location: https://synbiohub.org/public/igem/BBa_K1486010/annotation2425001/range2425001/1
component: BBa_K1486000

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persistent identity: range2425001
display id: range2425001
version: 1
direction: direction/reverse
top level: BBa_K1486010
start: 2372
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orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2424997
display id: annotation2424997
version: 1
top level: BBa_K1486010
location: https://synbiohub.org/public/igem/BBa_K1486010/annotation2424997/range2424997/1
component: BBa_B0034

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persistent identity: range2424997
display id: range2424997
version: 1
direction: direction/reverse
top level: BBa_K1486010
start: 2360
end: 2371
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BBa_K1486006
type: SequenceAnnotation
persistent identity: annotation2424990
display id: annotation2424990
version: 1
top level: BBa_K1486010
location: https://synbiohub.org/public/igem/BBa_K1486010/annotation2424990/range2424990/1
component: BBa_K1486006

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type: Range
persistent identity: range2424990
display id: range2424990
version: 1
direction: direction/reverse
top level: BBa_K1486010
start: 1223
end: 1627
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2424984
display id: annotation2424984
version: 1
top level: BBa_K1486010
location: https://synbiohub.org/public/igem/BBa_K1486010/annotation2424984/range2424984/1
component: BBa_I0500

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type: Range
persistent identity: range2424984
display id: range2424984
version: 1
direction: direction/reverse
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start: 1
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BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2424986
display id: annotation2424986
version: 1
top level: BBa_K1486010
location: https://synbiohub.org/public/igem/BBa_K1486010/annotation2424986/range2424986/1
component: BBa_B0034

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type: Range
persistent identity: range2424986
display id: range2424986
version: 1
direction: direction/reverse
top level: BBa_K1486010
start: 1211
end: 1222
orientation: inline

BBa_K1486007
type: SequenceAnnotation
persistent identity: annotation2425006
display id: annotation2425006
version: 1
top level: BBa_K1486010
location: https://synbiohub.org/public/igem/BBa_K1486010/annotation2425006/range2425006/1
component: BBa_K1486007

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type: Range
persistent identity: range2425006
display id: range2425006
version: 1
direction: direction/reverse
top level: BBa_K1486010
start: 3104
end: 3688
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2424995
display id: annotation2424995
version: 1
top level: BBa_K1486010
location: https://synbiohub.org/public/igem/BBa_K1486010/annotation2424995/range2424995/1
component: BBa_K1486000

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type: Range
persistent identity: range2424995
display id: range2424995
version: 1
direction: direction/reverse
top level: BBa_K1486010
start: 1658
end: 2359
orientation: inline

BBa_K1300000
type: ComponentDefinition
persistent identity: BBa K1300000
display id: BBa_K1300000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: recA promoter + Key (taRNA)
creator: StuyGem
created: 2014-06-15T11:00:00Z
modified: 2015-05-08T01:09:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1663_
m user id: 0
owner id: 19613
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: RecA is a UV-light-inducible promoter. Trans is a "key" of sorts, acting on a complementary cis "lock" to allow the translation of ccdB, a kill gene. Together, the RecA+Trans system allows for the kill gene to be translated in the presence of UV light, through the indirect induction by way of the cis-trans system.
mutable notes: We had to consider whether the design was too complicated, whether it wouldn't be better to just have a more simple, direct system whereby a kill gene was translated because of the action of a UV-light-inducible promoter. In the end, we decided to make both systems, as the latter has been done before and submitted to the iGEM Registry of Standard Biological Parts.
mutable provenance: Source parts were obtained from the iGEM Registry of Standard Biological Parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J22106
component: BBa_I714075
sequence annotation: BBa_I714075
sequence annotation: BBa_J22106
sequence: https://synbiohub.org/public/igem/BBa_K1300000_sequence/1

BBa_J22106
type: Component
persistent identity: component2379199
display id: component2379199
version: 1
top level: BBa_K1300000
access: public
definition: https://synbiohub.org/public/igem/BBa_J22106/1

BBa_I714075
type: Component
persistent identity: component2379207
display id: component2379207
version: 1
top level: BBa_K1300000
access: public
definition: https://synbiohub.org/public/igem/BBa_I714075/1

BBa_I714075
type: SequenceAnnotation
persistent identity: annotation2379207
display id: annotation2379207
version: 1
top level: BBa_K1300000
location: https://synbiohub.org/public/igem/BBa_K1300000/annotation2379207/range2379207/1
component: BBa_I714075

https://synbiohub.org/public/igem/BBa_K1300000/annotation2379207/range2379207/1
type: Range
persistent identity: range2379207
display id: range2379207
version: 1
direction: direction/reverse
top level: BBa_K1300000
start: 201
end: 431
orientation: inline

BBa_J22106
type: SequenceAnnotation
persistent identity: annotation2379199
display id: annotation2379199
version: 1
top level: BBa_K1300000
location: https://synbiohub.org/public/igem/BBa_K1300000/annotation2379199/range2379199/1
component: BBa_J22106

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type: Range
persistent identity: range2379199
display id: range2379199
version: 1
direction: direction/reverse
top level: BBa_K1300000
start: 1
end: 192
orientation: inline

BBa_K1486056
type: ComponentDefinition
persistent identity: BBa K1486056
display id: BBa_K1486056
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CxpR & Split IFP1.4 [Cterm + Cterm][2]
creator: iGem EPFL 2014
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is the twin of the non-compatible biobrick BBa_K1486008 rendered compatible by mutating the two illegal restriction sites. However, all experiments were run on the aforementioned biobrick.
mutable notes: Site-directed mutagenesis was used to remove the restriction sites in the sequence.
mutable provenance: http://www.addgene.org/browse/article/8177/ which was then mutated by us.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_B0034
component: BBa_K1486044
component: BBa_K1486045
component: BBa_K1486004
component: BBa_K1486000
component: BBa_I0500
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486044
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486000
sequence annotation: BBa_K1486045
sequence annotation: BBa_K1486004
sequence: https://synbiohub.org/public/igem/BBa_K1486056_sequence/1

BBa_B0034
type: Component
persistent identity: component2398601
display id: component2398601
version: 1
top level: BBa_K1486056
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486044
type: Component
persistent identity: component2398607
display id: component2398607
version: 1
top level: BBa_K1486056
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486044/1

BBa_K1486045
type: Component
persistent identity: component2398615
display id: component2398615
version: 1
top level: BBa_K1486056
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486045/1

BBa_K1486004
type: Component
persistent identity: component2398606
display id: component2398606
version: 1
top level: BBa_K1486056
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486000
type: Component
persistent identity: component2398605
display id: component2398605
version: 1
top level: BBa_K1486056
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_K1486004
type: Component
persistent identity: component2398614
display id: component2398614
version: 1
top level: BBa_K1486056
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486000
type: Component
persistent identity: component2398613
display id: component2398613
version: 1
top level: BBa_K1486056
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_B0034
type: Component
persistent identity: component2398609
display id: component2398609
version: 1
top level: BBa_K1486056
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I0500
type: Component
persistent identity: component2398599
display id: component2398599
version: 1
top level: BBa_K1486056
access: public
definition: pBad/araC

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2398609
display id: annotation2398609
version: 1
top level: BBa_K1486056
location: https://synbiohub.org/public/igem/BBa_K1486056/annotation2398609/range2398609/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486056/annotation2398609/range2398609/1
type: Range
persistent identity: range2398609
display id: range2398609
version: 1
direction: direction/reverse
top level: BBa_K1486056
start: 2395
end: 2406
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2398601
display id: annotation2398601
version: 1
top level: BBa_K1486056
location: https://synbiohub.org/public/igem/BBa_K1486056/annotation2398601/range2398601/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486056/annotation2398601/range2398601/1
type: Range
persistent identity: range2398601
display id: range2398601
version: 1
direction: direction/reverse
top level: BBa_K1486056
start: 1219
end: 1230
orientation: inline

BBa_K1486044
type: SequenceAnnotation
persistent identity: annotation2398607
display id: annotation2398607
version: 1
top level: BBa_K1486056
location: https://synbiohub.org/public/igem/BBa_K1486056/annotation2398607/range2398607/1
component: BBa_K1486044

https://synbiohub.org/public/igem/BBa_K1486056/annotation2398607/range2398607/1
type: Range
persistent identity: range2398607
display id: range2398607
version: 1
direction: direction/reverse
top level: BBa_K1486056
start: 1985
end: 2386
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2398599
display id: annotation2398599
version: 1
top level: BBa_K1486056
location: https://synbiohub.org/public/igem/BBa_K1486056/annotation2398599/range2398599/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486056/annotation2398599/range2398599/1
type: Range
persistent identity: range2398599
display id: range2398599
version: 1
direction: direction/reverse
top level: BBa_K1486056
start: 1
end: 1210
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2398613
display id: annotation2398613
version: 1
top level: BBa_K1486056
location: https://synbiohub.org/public/igem/BBa_K1486056/annotation2398613/range2398613/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486056/annotation2398613/range2398613/1
type: Range
persistent identity: range2398613
display id: range2398613
version: 1
direction: direction/reverse
top level: BBa_K1486056
start: 2413
end: 3114
orientation: inline

BBa_K1486045
type: SequenceAnnotation
persistent identity: annotation2398615
display id: annotation2398615
version: 1
top level: BBa_K1486056
location: https://synbiohub.org/public/igem/BBa_K1486056/annotation2398615/range2398615/1
component: BBa_K1486045

https://synbiohub.org/public/igem/BBa_K1486056/annotation2398615/range2398615/1
type: Range
persistent identity: range2398615
display id: range2398615
version: 1
direction: direction/reverse
top level: BBa_K1486056
start: 3161
end: 3742
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2398606
display id: annotation2398606
version: 1
top level: BBa_K1486056
location: https://synbiohub.org/public/igem/BBa_K1486056/annotation2398606/range2398606/1
component: BBa_K1486004

https://synbiohub.org/public/igem/BBa_K1486056/annotation2398606/range2398606/1
type: Range
persistent identity: range2398606
display id: range2398606
version: 1
direction: direction/reverse
top level: BBa_K1486056
start: 1947
end: 1976
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2398605
display id: annotation2398605
version: 1
top level: BBa_K1486056
location: https://synbiohub.org/public/igem/BBa_K1486056/annotation2398605/range2398605/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486056/annotation2398605/range2398605/1
type: Range
persistent identity: range2398605
display id: range2398605
version: 1
direction: direction/reverse
top level: BBa_K1486056
start: 1237
end: 1938
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2398614
display id: annotation2398614
version: 1
top level: BBa_K1486056
location: https://synbiohub.org/public/igem/BBa_K1486056/annotation2398614/range2398614/1
component: BBa_K1486004

https://synbiohub.org/public/igem/BBa_K1486056/annotation2398614/range2398614/1
type: Range
persistent identity: range2398614
display id: range2398614
version: 1
direction: direction/reverse
top level: BBa_K1486056
start: 3123
end: 3152
orientation: inline

BBa_K731201
type: ComponentDefinition
persistent identity: BBa K731201
display id: BBa_K731201
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Arabinose inducible araC-pBAD promoter
creator: Daniele Rossetto
created: 2012-08-02T11:00:00Z
modified: 2015-05-08T01:13:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _977_
m user id: 0
owner id: 11997
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: This E. coli promoter is positively regulated by arabinose. araC is the regulator protein, binding to arabinose prevents the repression of pBAD.
mutable notes: This part is similar to BBa_I0500, but it differs from that one in several point mutations in the sequence.
mutable provenance: E. coli genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: pBAD
sequence annotation: araC
sequence annotation: PC
sequence: https://synbiohub.org/public/igem/BBa_K731201_sequence/1

pBAD
type: SequenceAnnotation
persistent identity: annotation2179311
display id: annotation2179311
version: 1
top level: BBa_K731201
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K731201/annotation2179311/range2179311/1

https://synbiohub.org/public/igem/BBa_K731201/annotation2179311/range2179311/1
type: Range
persistent identity: range2179311
display id: range2179311
version: 1
direction: direction/forward
top level: BBa_K731201
start: 1080
end: 1203
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation2179313
display id: annotation2179313
version: 1
top level: BBa_K731201
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K731201/annotation2179313/range2179313/1

https://synbiohub.org/public/igem/BBa_K731201/annotation2179313/range2179313/1
type: Range
persistent identity: range2179313
display id: range2179313
version: 1
direction: direction/reverse
top level: BBa_K731201
start: 1
end: 1080
orientation: inline

PC
type: SequenceAnnotation
persistent identity: annotation2179312
display id: annotation2179312
version: 1
top level: BBa_K731201
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K731201/annotation2179312/range2179312/1

https://synbiohub.org/public/igem/BBa_K731201/annotation2179312/range2179312/1
type: Range
persistent identity: range2179312
display id: range2179312
version: 1
direction: direction/reverse
top level: BBa_K731201
start: 1043
end: 1114
orientation: inline

recNp
type: ComponentDefinition
persistent identity: BBa K629002
display id: BBa_K629002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: recNp, could be started with exposure to irradiation, UV, nalidixic acid
creator: Zilong WANG, Yi ZHENG
created: 2011-10-02T11:00:00Z
modified: 2015-05-08T01:12:56Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _801_
m user id: 0
owner id: 9022
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Name: recNp +1: 2749781 Sigma Factor: Sigma70 Sigmulon Distance from start of the gene: 36 Sequence: aattattctaattttacgccagcctctttactgtatataaaaccagtttatactgtacacAataacagtaatggtttttca -35 -10 +1 Note(s): We assigned a putative transcription start site to this promoter based on the observation that the majority of the promoters, whose transcription start sites were determined experimentally, present a distance of 6 nucleotides between the transcription start site and the -10 box. Evidence(s): [HIPP] Human inference of promoter position Reference(s): [1] Rostas K., et al., 1987
mutable notes: the -35 and -10 district, the RBS
mutable provenance: E. Coli BL21 plys genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: fwd primer
sequence annotation: recNp
sequence annotation: -35 box
sequence annotation: LexA
sequence annotation: -10 box
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_K629002_sequence/1

fwd primer
type: SequenceAnnotation
persistent identity: annotation2154338
display id: annotation2154338
version: 1
top level: recNp
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K629002/annotation2154338/range2154338/1

https://synbiohub.org/public/igem/BBa_K629002/annotation2154338/range2154338/1
type: Range
persistent identity: range2154338
display id: range2154338
version: 1
direction: direction/forward
top level: recNp
start: 1
end: 18
orientation: inline

recNp
type: SequenceAnnotation
persistent identity: annotation2154341
display id: annotation2154341
version: 1
top level: recNp
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K629002/annotation2154341/range2154341/1

https://synbiohub.org/public/igem/BBa_K629002/annotation2154341/range2154341/1
type: Range
persistent identity: range2154341
display id: range2154341
version: 1
direction: direction/reverse
top level: recNp
start: 108
end: 125
orientation: inline

-35 box
type: SequenceAnnotation
persistent identity: annotation2154339
display id: annotation2154339
version: 1
top level: recNp
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K629002/annotation2154339/range2154339/1

https://synbiohub.org/public/igem/BBa_K629002/annotation2154339/range2154339/1
type: Range
persistent identity: range2154339
display id: range2154339
version: 1
direction: direction/forward
top level: recNp
start: 58
end: 63
orientation: inline

LexA
type: SequenceAnnotation
persistent identity: annotation2154344
display id: annotation2154344
version: 1
top level: recNp
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K629002/annotation2154344/range2154344/1

https://synbiohub.org/public/igem/BBa_K629002/annotation2154344/range2154344/1
type: Range
persistent identity: range2154344
display id: range2154344
version: 1
direction: direction/forward
top level: recNp
start: 82
end: 101
orientation: inline

-10 box
type: SequenceAnnotation
persistent identity: annotation2154340
display id: annotation2154340
version: 1
top level: recNp
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K629002/annotation2154340/range2154340/1

https://synbiohub.org/public/igem/BBa_K629002/annotation2154340/range2154340/1
type: Range
persistent identity: range2154340
display id: range2154340
version: 1
direction: direction/forward
top level: recNp
start: 77
end: 85
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2154342
display id: annotation2154342
version: 1
top level: recNp
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K629002/annotation2154342/range2154342/1

https://synbiohub.org/public/igem/BBa_K629002/annotation2154342/range2154342/1
type: Range
persistent identity: range2154342
display id: range2154342
version: 1
direction: direction/forward
top level: recNp
start: 116
end: 119
orientation: inline

pBad/araC
type: ComponentDefinition
persistent identity: BBa I0500
display id: BBa_I0500
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Inducible pBad/araC promoter
creator: Sri Kosuri
created: 2003-12-05T12:00:00Z
modified: 2015-08-31T04:07:29Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: PC
sequence annotation: AraC
sequence annotation: Pbad
sequence: https://synbiohub.org/public/igem/BBa_I0500_sequence/1

PC
type: SequenceAnnotation
persistent identity: annotation1498842
display id: annotation1498842
version: 1
top level: pBad/araC
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498842/range1498842/1

https://synbiohub.org/public/igem/BBa_I0500/annotation1498842/range1498842/1
type: Range
persistent identity: range1498842
display id: range1498842
version: 1
direction: direction/forward
top level: pBad/araC
start: 1043
end: 1114
orientation: inline

AraC
type: SequenceAnnotation
persistent identity: annotation1498843
display id: annotation1498843
version: 1
top level: pBad/araC
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498843/range1498843/1

https://synbiohub.org/public/igem/BBa_I0500/annotation1498843/range1498843/1
type: Range
persistent identity: range1498843
display id: range1498843
version: 1
direction: direction/forward
top level: pBad/araC
start: 1
end: 1079
orientation: inline

Pbad
type: SequenceAnnotation
persistent identity: annotation1498841
display id: annotation1498841
version: 1
top level: pBad/araC
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498841/range1498841/1

https://synbiohub.org/public/igem/BBa_I0500/annotation1498841/range1498841/1
type: Range
persistent identity: range1498841
display id: range1498841
version: 1
direction: direction/forward
top level: pBad/araC
start: 1080
end: 1210
orientation: inline

Pgad
type: ComponentDefinition
persistent identity: BBa K559011
display id: BBa_K559011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pgad - Intracellular Chloride-Sensing Cassette
creator: Jacky, Fong Chuen, Loo
created: 2011-10-27T11:00:00Z
modified: 2015-05-08T01:12:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _727_
m user id: 0
owner id: 6123
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Pgad is chloride-sensitive promoter which was first discovered in Lactococcus lactis1, which is a gram-positive bacterium which can live in acidic environment. In this operon, gadC is glutamate-gamma-aminobutyrate antiporter and gadB is glutamate decarboxylase. They are both involved in intracellular pH regulation and co-expressed in the same operon under the control of Pgad2. The gene before Pgad, named gadR, which is constitutively expressed under the control of PgadR, is a positive regulator of Pgad coupled genes while intracellular chloride is level elevated.
mutable notes: Synthesized part by GenScript,all the illegal sites for RFC 10 (EcoRI, XbaI, SpeI, PstI) have been substituted by other codon producing the same amino acid in E. coli.
mutable provenance: The gene sequence was obtained from the cassette from bp 821 to 2071 of GenBank sequence AF005098, which includes PgadR, gadR, Pgad and the starting codon ATG.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Pgad
sequence annotation: RBS_gadR
sequence annotation: RBS_gadC
sequence annotation: gadC Start
sequence annotation: gadR
sequence annotation: PgadR
sequence: https://synbiohub.org/public/igem/BBa_K559011_sequence/1

Pgad
type: SequenceAnnotation
persistent identity: annotation2160939
display id: annotation2160939
version: 1
top level: Pgad
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K559011/annotation2160939/range2160939/1

https://synbiohub.org/public/igem/BBa_K559011/annotation2160939/range2160939/1
type: Range
persistent identity: range2160939
display id: range2160939
version: 1
direction: direction/forward
top level: Pgad
start: 1163
end: 1181
orientation: inline

RBS_gadR
type: SequenceAnnotation
persistent identity: annotation2160941
display id: annotation2160941
version: 1
top level: Pgad
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K559011/annotation2160941/range2160941/1

https://synbiohub.org/public/igem/BBa_K559011/annotation2160941/range2160941/1
type: Range
persistent identity: range2160941
display id: range2160941
version: 1
direction: direction/forward
top level: Pgad
start: 264
end: 269
orientation: inline

RBS_gadC
type: SequenceAnnotation
persistent identity: annotation2160940
display id: annotation2160940
version: 1
top level: Pgad
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K559011/annotation2160940/range2160940/1

https://synbiohub.org/public/igem/BBa_K559011/annotation2160940/range2160940/1
type: Range
persistent identity: range2160940
display id: range2160940
version: 1
direction: direction/forward
top level: Pgad
start: 1237
end: 1245
orientation: inline

gadC Start
type: SequenceAnnotation
persistent identity: annotation2160936
display id: annotation2160936
version: 1
top level: Pgad
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K559011/annotation2160936/range2160936/1

https://synbiohub.org/public/igem/BBa_K559011/annotation2160936/range2160936/1
type: Range
persistent identity: range2160936
display id: range2160936
version: 1
direction: direction/forward
top level: Pgad
start: 1249
end: 1251
orientation: inline

gadR
type: SequenceAnnotation
persistent identity: annotation2160938
display id: annotation2160938
version: 1
top level: Pgad
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K559011/annotation2160938/range2160938/1

https://synbiohub.org/public/igem/BBa_K559011/annotation2160938/range2160938/1
type: Range
persistent identity: range2160938
display id: range2160938
version: 1
direction: direction/forward
top level: Pgad
start: 275
end: 1105
orientation: inline

PgadR
type: SequenceAnnotation
persistent identity: annotation2160937
display id: annotation2160937
version: 1
top level: Pgad
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K559011/annotation2160937/range2160937/1

https://synbiohub.org/public/igem/BBa_K559011/annotation2160937/range2160937/1
type: Range
persistent identity: range2160937
display id: range2160937
version: 1
direction: direction/forward
top level: Pgad
start: 141
end: 158
orientation: inline

BBa_K1486013
type: ComponentDefinition
persistent identity: BBa K1486013
display id: BBa_K1486013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CpxR-IFP[2] under pBAD
creator: iGem EPFL 2014
created: 2014-08-31T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has a fused protein downstream of an arabinose-inducible promoter: CpxR-IFP[2]. Biobrick BBa_K1486012 has CpxR-IFP[1] with an arabinose promoter. This construct was made in both orientations: BBa_K1486015 is IFP[2]-CpxR (with an arabinose promoter).
mutable notes: Both orientations were made, as well as for IFP[1] so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used to fuse both proteins, and an RBS was put before each one of them.
mutable provenance: to edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486004
component: BBa_I0500
component: BBa_K1486000
component: BBa_K1486007
component: BBa_B0034
sequence annotation: BBa_K1486007
sequence annotation: BBa_B0034
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486004
sequence annotation: BBa_K1486000
sequence: https://synbiohub.org/public/igem/BBa_K1486013_sequence/1

BBa_K1486004
type: Component
persistent identity: component2382304
display id: component2382304
version: 1
top level: BBa_K1486013
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_I0500
type: Component
persistent identity: component2382297
display id: component2382297
version: 1
top level: BBa_K1486013
access: public
definition: pBad/araC

BBa_K1486000
type: Component
persistent identity: component2382303
display id: component2382303
version: 1
top level: BBa_K1486013
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_K1486007
type: Component
persistent identity: component2382308
display id: component2382308
version: 1
top level: BBa_K1486013
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486007/1

BBa_B0034
type: Component
persistent identity: component2382299
display id: component2382299
version: 1
top level: BBa_K1486013
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486007
type: SequenceAnnotation
persistent identity: annotation2382308
display id: annotation2382308
version: 1
top level: BBa_K1486013
location: https://synbiohub.org/public/igem/BBa_K1486013/annotation2382308/range2382308/1
component: BBa_K1486007

https://synbiohub.org/public/igem/BBa_K1486013/annotation2382308/range2382308/1
type: Range
persistent identity: range2382308
display id: range2382308
version: 1
direction: direction/reverse
top level: BBa_K1486013
start: 1955
end: 2533
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2382299
display id: annotation2382299
version: 1
top level: BBa_K1486013
location: https://synbiohub.org/public/igem/BBa_K1486013/annotation2382299/range2382299/1
component: BBa_B0034

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type: Range
persistent identity: range2382299
display id: range2382299
version: 1
direction: direction/reverse
top level: BBa_K1486013
start: 1211
end: 1222
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2382297
display id: annotation2382297
version: 1
top level: BBa_K1486013
location: https://synbiohub.org/public/igem/BBa_K1486013/annotation2382297/range2382297/1
component: BBa_I0500

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type: Range
persistent identity: range2382297
display id: range2382297
version: 1
direction: direction/reverse
top level: BBa_K1486013
start: 1
end: 1210
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2382304
display id: annotation2382304
version: 1
top level: BBa_K1486013
location: https://synbiohub.org/public/igem/BBa_K1486013/annotation2382304/range2382304/1
component: BBa_K1486004

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type: Range
persistent identity: range2382304
display id: range2382304
version: 1
direction: direction/reverse
top level: BBa_K1486013
start: 1925
end: 1954
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2382303
display id: annotation2382303
version: 1
top level: BBa_K1486013
location: https://synbiohub.org/public/igem/BBa_K1486013/annotation2382303/range2382303/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486013/annotation2382303/range2382303/1
type: Range
persistent identity: range2382303
display id: range2382303
version: 1
direction: direction/reverse
top level: BBa_K1486013
start: 1223
end: 1924
orientation: inline

BBa_K1486012
type: ComponentDefinition
persistent identity: BBa K1486012
display id: BBa_K1486012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CpxR-IFP[1] under pBAD
creator: iGem EPFL 2014
created: 2014-08-31T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has a fused protein downstream of an arabinose-inducible promoter: CpxR-IFP[1]. Biobrick BBa_K1486013 has CpxR-IFP[2] with an arabinose promoter. This construct was made in both orientations: BBa_K1486014 is IFP[1]-CpxR (with an arabinose promoter).
mutable notes: Both orientations were made, as well as for IFP[2] so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used to fuse both proteins, and an RBS was put before each one of them.
mutable provenance: To Edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486004
component: BBa_K1486000
component: BBa_I0500
component: BBa_B0034
component: BBa_K1486006
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486004
sequence annotation: BBa_K1486000
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486006
sequence: https://synbiohub.org/public/igem/BBa_K1486012_sequence/1

BBa_K1486004
type: Component
persistent identity: component2382292
display id: component2382292
version: 1
top level: BBa_K1486012
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486000
type: Component
persistent identity: component2382291
display id: component2382291
version: 1
top level: BBa_K1486012
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_I0500
type: Component
persistent identity: component2382285
display id: component2382285
version: 1
top level: BBa_K1486012
access: public
definition: pBad/araC

BBa_B0034
type: Component
persistent identity: component2382287
display id: component2382287
version: 1
top level: BBa_K1486012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486006
type: Component
persistent identity: component2382293
display id: component2382293
version: 1
top level: BBa_K1486012
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486006/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2382287
display id: annotation2382287
version: 1
top level: BBa_K1486012
location: https://synbiohub.org/public/igem/BBa_K1486012/annotation2382287/range2382287/1
component: BBa_B0034

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type: Range
persistent identity: range2382287
display id: range2382287
version: 1
direction: direction/reverse
top level: BBa_K1486012
start: 1211
end: 1222
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2382292
display id: annotation2382292
version: 1
top level: BBa_K1486012
location: https://synbiohub.org/public/igem/BBa_K1486012/annotation2382292/range2382292/1
component: BBa_K1486004

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type: Range
persistent identity: range2382292
display id: range2382292
version: 1
direction: direction/reverse
top level: BBa_K1486012
start: 1925
end: 1954
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2382291
display id: annotation2382291
version: 1
top level: BBa_K1486012
location: https://synbiohub.org/public/igem/BBa_K1486012/annotation2382291/range2382291/1
component: BBa_K1486000

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type: Range
persistent identity: range2382291
display id: range2382291
version: 1
direction: direction/reverse
top level: BBa_K1486012
start: 1223
end: 1924
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2382285
display id: annotation2382285
version: 1
top level: BBa_K1486012
location: https://synbiohub.org/public/igem/BBa_K1486012/annotation2382285/range2382285/1
component: BBa_I0500

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type: Range
persistent identity: range2382285
display id: range2382285
version: 1
direction: direction/reverse
top level: BBa_K1486012
start: 1
end: 1210
orientation: inline

BBa_K1486006
type: SequenceAnnotation
persistent identity: annotation2382293
display id: annotation2382293
version: 1
top level: BBa_K1486012
location: https://synbiohub.org/public/igem/BBa_K1486012/annotation2382293/range2382293/1
component: BBa_K1486006

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type: Range
persistent identity: range2382293
display id: range2382293
version: 1
direction: direction/reverse
top level: BBa_K1486012
start: 1955
end: 2536
orientation: inline

BBa_K332031
type: ComponentDefinition
persistent identity: BBa K332031
display id: BBa_K332031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive promoter + 37℃ induced RBS + tetR + double terminator
creator: KUO, CHIA-CHUN
created: 2010-10-21T11:00:00Z
modified: 2015-05-08T01:12:05Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _452_
m user id: 0
owner id: 7059
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: we took advantage of the interaction between 37?? C induced RBS, tetR gene and pTet promoter to create the temperature regulating circuit. The following is a description of our prescribed strategy. First, we use the constitutive promoter, 37?? C induced RBS (K115002), the tetR gene and terminators. We will call this part the ???Regulator???. We speculate that when the ???Regulator??? and ???Production??? sections are combined, we can achieve a high bacteria growth rate and close to no target protein producing rate at T>37??C, and low bacteria growth rate and high target protein producing rate at T<37??C.
mutable notes: To regulate following gene by temperature
mutable provenance: iGEM08_TUDelft
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_C0040
component: BBa_K115002
component: BBa_J23101
component: BBa_J61048
sequence annotation: BBa_J61048
sequence annotation: BBa_J23101
sequence annotation: BBa_K115002
sequence annotation: BBa_C0040
sequence: https://synbiohub.org/public/igem/BBa_K332031_sequence/1

BBa_C0040
type: Component
persistent identity: component2250406
display id: component2250406
version: 1
top level: BBa_K332031
access: public
definition: https://synbiohub.org/public/igem/BBa_C0040/1

BBa_K115002
type: Component
persistent identity: component2250402
display id: component2250402
version: 1
top level: BBa_K332031
access: public
definition: https://synbiohub.org/public/igem/BBa_K115002/1

BBa_J23101
type: Component
persistent identity: component2250397
display id: component2250397
version: 1
top level: BBa_K332031
access: public
definition: https://synbiohub.org/public/igem/BBa_J23101/1

BBa_J61048
type: Component
persistent identity: component2250407
display id: component2250407
version: 1
top level: BBa_K332031
access: public
definition: https://synbiohub.org/public/igem/BBa_J61048/1

BBa_J61048
type: SequenceAnnotation
persistent identity: annotation2250407
display id: annotation2250407
version: 1
top level: BBa_K332031
location: https://synbiohub.org/public/igem/BBa_K332031/annotation2250407/range2250407/1
component: BBa_J61048

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type: Range
persistent identity: range2250407
display id: range2250407
version: 1
direction: direction/reverse
top level: BBa_K332031
start: 795
end: 907
orientation: inline

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2250397
display id: annotation2250397
version: 1
top level: BBa_K332031
location: https://synbiohub.org/public/igem/BBa_K332031/annotation2250397/range2250397/1
component: BBa_J23101

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type: Range
persistent identity: range2250397
display id: range2250397
version: 1
direction: direction/reverse
top level: BBa_K332031
start: 1
end: 35
orientation: inline

BBa_K115002
type: SequenceAnnotation
persistent identity: annotation2250402
display id: annotation2250402
version: 1
top level: BBa_K332031
location: https://synbiohub.org/public/igem/BBa_K332031/annotation2250402/range2250402/1
component: BBa_K115002

https://synbiohub.org/public/igem/BBa_K332031/annotation2250402/range2250402/1
type: Range
persistent identity: range2250402
display id: range2250402
version: 1
direction: direction/reverse
top level: BBa_K332031
start: 44
end: 95
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2250406
display id: annotation2250406
version: 1
top level: BBa_K332031
location: https://synbiohub.org/public/igem/BBa_K332031/annotation2250406/range2250406/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K332031/annotation2250406/range2250406/1
type: Range
persistent identity: range2250406
display id: range2250406
version: 1
direction: direction/reverse
top level: BBa_K332031
start: 102
end: 786
orientation: inline

BBa_K1486011
type: ComponentDefinition
persistent identity: BBa K1486011
display id: BBa_K1486011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CxpR & Split IFP1.4 [Cterm + Nterm]
creator: iGem EPFL 2014
created: 2014-08-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has two fused proteins downstream of an arabinose-inducible promoter: CpxR-IFP[1] and CpxR-IFP[2]. This construct was made in all orientations: - CpxR-IFP[1] + CpxR-IFP[2] (BBa_K1286008) - IFP[1]-CpxR + CpxR-IFP[2] (BBa_K1486010) - CpxR-IFP[1] + IFP[2]-CpxR (BBa_K1486011)
mutable notes: All orientations were made, so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used for each of the fused proteins, and an RBS was put before each one of them.
mutable provenance: From Michnick Article. To Edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486004
component: BBa_K1486006
component: BBa_B0034
component: BBa_K1486000
component: BBa_I0500
component: BBa_K1486007
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486006
sequence annotation: BBa_K1486000
sequence annotation: BBa_K1486007
sequence annotation: BBa_K1486004
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1486011_sequence/1

BBa_K1486004
type: Component
persistent identity: component2382250
display id: component2382250
version: 1
top level: BBa_K1486011
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486006
type: Component
persistent identity: component2382243
display id: component2382243
version: 1
top level: BBa_K1486011
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486006/1

BBa_B0034
type: Component
persistent identity: component2382245
display id: component2382245
version: 1
top level: BBa_K1486011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component2382237
display id: component2382237
version: 1
top level: BBa_K1486011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486000
type: Component
persistent identity: component2382254
display id: component2382254
version: 1
top level: BBa_K1486011
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_K1486000
type: Component
persistent identity: component2382241
display id: component2382241
version: 1
top level: BBa_K1486011
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_K1486004
type: Component
persistent identity: component2382242
display id: component2382242
version: 1
top level: BBa_K1486011
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_I0500
type: Component
persistent identity: component2382235
display id: component2382235
version: 1
top level: BBa_K1486011
access: public
definition: pBad/araC

BBa_K1486007
type: Component
persistent identity: component2382249
display id: component2382249
version: 1
top level: BBa_K1486011
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486007/1

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2382235
display id: annotation2382235
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382235/range2382235/1
component: BBa_I0500

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type: Range
persistent identity: range2382235
display id: range2382235
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 1
end: 1210
orientation: inline

BBa_K1486006
type: SequenceAnnotation
persistent identity: annotation2382243
display id: annotation2382243
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382243/range2382243/1
component: BBa_K1486006

https://synbiohub.org/public/igem/BBa_K1486011/annotation2382243/range2382243/1
type: Range
persistent identity: range2382243
display id: range2382243
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 1955
end: 2536
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2382254
display id: annotation2382254
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382254/range2382254/1
component: BBa_K1486000

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type: Range
persistent identity: range2382254
display id: range2382254
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 3158
end: 3859
orientation: inline

BBa_K1486007
type: SequenceAnnotation
persistent identity: annotation2382249
display id: annotation2382249
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382249/range2382249/1
component: BBa_K1486007

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type: Range
persistent identity: range2382249
display id: range2382249
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 2549
end: 3127
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2382241
display id: annotation2382241
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382241/range2382241/1
component: BBa_K1486000

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type: Range
persistent identity: range2382241
display id: range2382241
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 1223
end: 1924
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2382250
display id: annotation2382250
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382250/range2382250/1
component: BBa_K1486004

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type: Range
persistent identity: range2382250
display id: range2382250
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 3128
end: 3157
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2382237
display id: annotation2382237
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382237/range2382237/1
component: BBa_B0034

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type: Range
persistent identity: range2382237
display id: range2382237
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 1211
end: 1222
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2382245
display id: annotation2382245
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382245/range2382245/1
component: BBa_B0034

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type: Range
persistent identity: range2382245
display id: range2382245
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 2537
end: 2548
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2382242
display id: annotation2382242
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382242/range2382242/1
component: BBa_K1486004

https://synbiohub.org/public/igem/BBa_K1486011/annotation2382242/range2382242/1
type: Range
persistent identity: range2382242
display id: range2382242
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 1925
end: 1954
orientation: inline

BBa_K1486054
type: ComponentDefinition
persistent identity: BBa K1486054
display id: BBa_K1486054
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CheY/CheZ + split rLuc
creator: EPFL iGem team 2014
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: to edit
mutable notes: to edit
mutable provenance: to edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_B0034
component: BBa_K1486053
component: BBa_I0500
component: BBa_K629003
component: BBa_K569017
component: BBa_K1486019
component: BBa_K1486020
sequence annotation: BBa_K569017
sequence annotation: BBa_K1486053
sequence annotation: BBa_K1486020
sequence annotation: BBa_B0034
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486019
sequence annotation: BBa_K629003
sequence: https://synbiohub.org/public/igem/BBa_K1486054_sequence/1

BBa_B0034
type: Component
persistent identity: component2399861
display id: component2399861
version: 1
top level: BBa_K1486054
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486053
type: Component
persistent identity: component2399874
display id: component2399874
version: 1
top level: BBa_K1486054
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486053/1

BBa_I0500
type: Component
persistent identity: component2399859
display id: component2399859
version: 1
top level: BBa_K1486054
access: public
definition: pBad/araC

BBa_K629003
type: Component
persistent identity: component2399873
display id: component2399873
version: 1
top level: BBa_K1486054
access: public
definition: https://synbiohub.org/public/igem/BBa_K629003/1

BBa_K569017
type: Component
persistent identity: component2399862
display id: component2399862
version: 1
top level: BBa_K1486054
access: public
definition: https://synbiohub.org/public/igem/BBa_K569017/1

BBa_K1486019
type: Component
persistent identity: component2399864
display id: component2399864
version: 1
top level: BBa_K1486054
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486019/1

BBa_B0034
type: Component
persistent identity: component2399866
display id: component2399866
version: 1
top level: BBa_K1486054
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486020
type: Component
persistent identity: component2399875
display id: component2399875
version: 1
top level: BBa_K1486054
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486020/1

BBa_K1486053
type: Component
persistent identity: component2399863
display id: component2399863
version: 1
top level: BBa_K1486054
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486053/1

BBa_K569017
type: SequenceAnnotation
persistent identity: annotation2399862
display id: annotation2399862
version: 1
top level: BBa_K1486054
location: https://synbiohub.org/public/igem/BBa_K1486054/annotation2399862/range2399862/1
component: BBa_K569017

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type: Range
persistent identity: range2399862
display id: range2399862
version: 1
direction: direction/reverse
top level: BBa_K1486054
start: 1223
end: 1640
orientation: inline

BBa_K1486053
type: SequenceAnnotation
persistent identity: annotation2399874
display id: annotation2399874
version: 1
top level: BBa_K1486054
location: https://synbiohub.org/public/igem/BBa_K1486054/annotation2399874/range2399874/1
component: BBa_K1486053

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persistent identity: range2399874
display id: range2399874
version: 1
direction: direction/reverse
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start: 2658
end: 2687
orientation: inline

BBa_K1486020
type: SequenceAnnotation
persistent identity: annotation2399875
display id: annotation2399875
version: 1
top level: BBa_K1486054
location: https://synbiohub.org/public/igem/BBa_K1486054/annotation2399875/range2399875/1
component: BBa_K1486020

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type: Range
persistent identity: range2399875
display id: range2399875
version: 1
direction: direction/reverse
top level: BBa_K1486054
start: 2688
end: 3293
orientation: inline

BBa_K1486053
type: SequenceAnnotation
persistent identity: annotation2399863
display id: annotation2399863
version: 1
top level: BBa_K1486054
location: https://synbiohub.org/public/igem/BBa_K1486054/annotation2399863/range2399863/1
component: BBa_K1486053

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type: Range
persistent identity: range2399863
display id: range2399863
version: 1
direction: direction/reverse
top level: BBa_K1486054
start: 1641
end: 1670
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2399866
display id: annotation2399866
version: 1
top level: BBa_K1486054
location: https://synbiohub.org/public/igem/BBa_K1486054/annotation2399866/range2399866/1
component: BBa_B0034

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type: Range
persistent identity: range2399866
display id: range2399866
version: 1
direction: direction/reverse
top level: BBa_K1486054
start: 2001
end: 2012
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2399859
display id: annotation2399859
version: 1
top level: BBa_K1486054
location: https://synbiohub.org/public/igem/BBa_K1486054/annotation2399859/range2399859/1
component: BBa_I0500

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type: Range
persistent identity: range2399859
display id: range2399859
version: 1
direction: direction/reverse
top level: BBa_K1486054
start: 1
end: 1210
orientation: inline

BBa_K1486019
type: SequenceAnnotation
persistent identity: annotation2399864
display id: annotation2399864
version: 1
top level: BBa_K1486054
location: https://synbiohub.org/public/igem/BBa_K1486054/annotation2399864/range2399864/1
component: BBa_K1486019

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type: Range
persistent identity: range2399864
display id: range2399864
version: 1
direction: direction/reverse
top level: BBa_K1486054
start: 1671
end: 2000
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2399861
display id: annotation2399861
version: 1
top level: BBa_K1486054
location: https://synbiohub.org/public/igem/BBa_K1486054/annotation2399861/range2399861/1
component: BBa_B0034

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type: Range
persistent identity: range2399861
display id: range2399861
version: 1
direction: direction/reverse
top level: BBa_K1486054
start: 1211
end: 1222
orientation: inline

BBa_K629003
type: SequenceAnnotation
persistent identity: annotation2399873
display id: annotation2399873
version: 1
top level: BBa_K1486054
location: https://synbiohub.org/public/igem/BBa_K1486054/annotation2399873/range2399873/1
component: BBa_K629003

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type: Range
persistent identity: range2399873
display id: range2399873
version: 1
direction: direction/reverse
top level: BBa_K1486054
start: 2013
end: 2657
orientation: inline

BBa_K1486014
type: ComponentDefinition
persistent identity: BBa K1486014
display id: BBa_K1486014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IFP[1]-CpxR under pBAD
creator: iGem EPFL 2014
created: 2014-08-31T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has a fused protein downstream of an arabinose-inducible promoter: IFP[1]-CpxR. Biobrick BBa_K1486015 has IFP[2]-CpxR with an arabinose promoter. This construct was made in both orientations: BBa_K1486012 is CpxR-IFP[1] (with an arabinose promoter).
mutable notes: Both orientations were made, as well as for IFP[2] so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used to fuse both proteins, and an RBS was put between the promoter and the cds.
mutable provenance: to edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486000
component: BBa_K1486006
component: BBa_I0500
component: BBa_B0034
component: BBa_K1486004
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486004
sequence annotation: BBa_K1486006
sequence annotation: BBa_K1486000
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1486014_sequence/1

BBa_K1486000
type: Component
persistent identity: component2424747
display id: component2424747
version: 1
top level: BBa_K1486014
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_K1486006
type: Component
persistent identity: component2424742
display id: component2424742
version: 1
top level: BBa_K1486014
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486006/1

BBa_I0500
type: Component
persistent identity: component2424736
display id: component2424736
version: 1
top level: BBa_K1486014
access: public
definition: pBad/araC

BBa_B0034
type: Component
persistent identity: component2424738
display id: component2424738
version: 1
top level: BBa_K1486014
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486004
type: Component
persistent identity: component2424743
display id: component2424743
version: 1
top level: BBa_K1486014
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2424736
display id: annotation2424736
version: 1
top level: BBa_K1486014
location: https://synbiohub.org/public/igem/BBa_K1486014/annotation2424736/range2424736/1
component: BBa_I0500

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type: Range
persistent identity: range2424736
display id: range2424736
version: 1
direction: direction/reverse
top level: BBa_K1486014
start: 1
end: 1210
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2424743
display id: annotation2424743
version: 1
top level: BBa_K1486014
location: https://synbiohub.org/public/igem/BBa_K1486014/annotation2424743/range2424743/1
component: BBa_K1486004

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type: Range
persistent identity: range2424743
display id: range2424743
version: 1
direction: direction/reverse
top level: BBa_K1486014
start: 1628
end: 1657
orientation: inline

BBa_K1486006
type: SequenceAnnotation
persistent identity: annotation2424742
display id: annotation2424742
version: 1
top level: BBa_K1486014
location: https://synbiohub.org/public/igem/BBa_K1486014/annotation2424742/range2424742/1
component: BBa_K1486006

https://synbiohub.org/public/igem/BBa_K1486014/annotation2424742/range2424742/1
type: Range
persistent identity: range2424742
display id: range2424742
version: 1
direction: direction/reverse
top level: BBa_K1486014
start: 1223
end: 1627
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2424747
display id: annotation2424747
version: 1
top level: BBa_K1486014
location: https://synbiohub.org/public/igem/BBa_K1486014/annotation2424747/range2424747/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486014/annotation2424747/range2424747/1
type: Range
persistent identity: range2424747
display id: range2424747
version: 1
direction: direction/reverse
top level: BBa_K1486014
start: 1658
end: 2359
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2424738
display id: annotation2424738
version: 1
top level: BBa_K1486014
location: https://synbiohub.org/public/igem/BBa_K1486014/annotation2424738/range2424738/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486014/annotation2424738/range2424738/1
type: Range
persistent identity: range2424738
display id: range2424738
version: 1
direction: direction/reverse
top level: BBa_K1486014
start: 1211
end: 1222
orientation: inline

recAp
type: ComponentDefinition
persistent identity: BBa K629001
display id: BBa_K629001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: recAp, could be started with exposure to irradiation, UV, nalidixic acid
creator: Zilong Wang, Yi ZHENG
created: 2011-10-02T11:00:00Z
modified: 2015-05-08T01:12:56Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _801_
m user id: 0
owner id: 9022
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Name: recAp +1: 2821841 Sigma Factor: Sigma70 Sigmulon Distance from start of the gene: 50 Sequence: cttgtggcaacaatttctacaaaacacttgatactgtatgagcatacagtataattgcttCaacagaacatattgactatc Evidence(s): [HIPP] Human inference of promoter position [TIM] Transcription initiation mapping Reference(s): [1] Pages V., et al., 2003
mutable notes: the -35 and -10 district, the RBS
mutable provenance: E. Coli BL21 plys genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: fwd primer
sequence annotation: RBS
sequence annotation: LexA
sequence annotation: -35 box
sequence annotation: -10 box
sequence annotation: rev primer
sequence: https://synbiohub.org/public/igem/BBa_K629001_sequence/1

fwd primer
type: SequenceAnnotation
persistent identity: annotation2154312
display id: annotation2154312
version: 1
top level: recAp
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K629001/annotation2154312/range2154312/1

https://synbiohub.org/public/igem/BBa_K629001/annotation2154312/range2154312/1
type: Range
persistent identity: range2154312
display id: range2154312
version: 1
direction: direction/forward
top level: recAp
start: 1
end: 17
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2154315
display id: annotation2154315
version: 1
top level: recAp
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K629001/annotation2154315/range2154315/1

https://synbiohub.org/public/igem/BBa_K629001/annotation2154315/range2154315/1
type: Range
persistent identity: range2154315
display id: range2154315
version: 1
direction: direction/forward
top level: recAp
start: 106
end: 109
orientation: inline

LexA
type: SequenceAnnotation
persistent identity: annotation2154313
display id: annotation2154313
version: 1
top level: recAp
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K629001/annotation2154313/range2154313/1

https://synbiohub.org/public/igem/BBa_K629001/annotation2154313/range2154313/1
type: Range
persistent identity: range2154313
display id: range2154313
version: 1
direction: direction/forward
top level: recAp
start: 37
end: 56
orientation: inline

-35 box
type: SequenceAnnotation
persistent identity: annotation2154500
display id: annotation2154500
version: 1
top level: recAp
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K629001/annotation2154500/range2154500/1

https://synbiohub.org/public/igem/BBa_K629001/annotation2154500/range2154500/1
type: Range
persistent identity: range2154500
display id: range2154500
version: 1
direction: direction/forward
top level: recAp
start: 33
end: 38
orientation: inline

-10 box
type: SequenceAnnotation
persistent identity: annotation2154499
display id: annotation2154499
version: 1
top level: recAp
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K629001/annotation2154499/range2154499/1

https://synbiohub.org/public/igem/BBa_K629001/annotation2154499/range2154499/1
type: Range
persistent identity: range2154499
display id: range2154499
version: 1
direction: direction/forward
top level: recAp
start: 52
end: 60
orientation: inline

rev primer
type: SequenceAnnotation
persistent identity: annotation2154314
display id: annotation2154314
version: 1
top level: recAp
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K629001/annotation2154314/range2154314/1

https://synbiohub.org/public/igem/BBa_K629001/annotation2154314/range2154314/1
type: Range
persistent identity: range2154314
display id: range2154314
version: 1
direction: direction/reverse
top level: recAp
start: 105
end: 124
orientation: inline

BBa_K772004
type: ComponentDefinition
persistent identity: BBa K772004
display id: BBa_K772004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AHL regulated PoPS generator: Las receiver device
creator: Mustafa Elitok
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
group u list: _1024_
m user id: 0
owner id: 9573
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Quorum sensing is the mechanism of communication between bacteria via synthesizing and detecting chemical compounds in the media. With these mediators, the promoters and their yield can be tuned or regulated. This part represents an alternative quorum sensing receiver device, which is able to generate a PoPS response in the presence of 6HSLO3 produced by LasI, instead of BBa_F2620. This will allow iGEMers to establish communications between different bacteria colonies and it makes simple to generate easy regulated complex systems. This part is assembled with Las promoter system to improve the current quorum sensing devices and parts which commonly base on Lux promoter system.
mutable notes: Note that pLas has no RBS afterwards, the assembling methods must be selected after this consideration.
mutable provenance: pLas and LasR sequences are gathered from Registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: Double Terminator
sequence annotation: pLas
sequence annotation: pTetR
sequence annotation: RBS
sequence annotation: LasR
sequence: https://synbiohub.org/public/igem/BBa_K772004_sequence/1

Double Terminator
type: SequenceAnnotation
persistent identity: annotation2203690
display id: annotation2203690
version: 1
top level: BBa_K772004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K772004/annotation2203690/range2203690/1

https://synbiohub.org/public/igem/BBa_K772004/annotation2203690/range2203690/1
type: Range
persistent identity: range2203690
display id: range2203690
version: 1
direction: direction/forward
top level: BBa_K772004
start: 825
end: 954
orientation: inline

pLas
type: SequenceAnnotation
persistent identity: annotation2203691
display id: annotation2203691
version: 1
top level: BBa_K772004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K772004/annotation2203691/range2203691/1

https://synbiohub.org/public/igem/BBa_K772004/annotation2203691/range2203691/1
type: Range
persistent identity: range2203691
display id: range2203691
version: 1
direction: direction/forward
top level: BBa_K772004
start: 955
end: 1122
orientation: inline

pTetR
type: SequenceAnnotation
persistent identity: annotation2203687
display id: annotation2203687
version: 1
top level: BBa_K772004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K772004/annotation2203687/range2203687/1

https://synbiohub.org/public/igem/BBa_K772004/annotation2203687/range2203687/1
type: Range
persistent identity: range2203687
display id: range2203687
version: 1
direction: direction/forward
top level: BBa_K772004
start: 1
end: 54
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2203688
display id: annotation2203688
version: 1
top level: BBa_K772004
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K772004/annotation2203688/range2203688/1

https://synbiohub.org/public/igem/BBa_K772004/annotation2203688/range2203688/1
type: Range
persistent identity: range2203688
display id: range2203688
version: 1
direction: direction/forward
top level: BBa_K772004
start: 55
end: 75
orientation: inline

LasR
type: SequenceAnnotation
persistent identity: annotation2203689
display id: annotation2203689
version: 1
top level: BBa_K772004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772004/annotation2203689/range2203689/1

https://synbiohub.org/public/igem/BBa_K772004/annotation2203689/range2203689/1
type: Range
persistent identity: range2203689
display id: range2203689
version: 1
direction: direction/forward
top level: BBa_K772004
start: 76
end: 824
orientation: inline