//classic/project/uncategorized
type: Collection
persistent identity: classic project uncategorized
display id: classic_project_uncategorized
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_I4102
member: BBa_J06952
member: https://synbiohub.org/public/igem/BBa_J13015/1
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member: https://synbiohub.org/public/igem/BBa_I9023/1
member: BBa_I14047
member: BBa_I9101
member: BBa_I3425
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member: BBa_I13537
member: BBa_I3001
member: BBa_I9030
member: https://synbiohub.org/public/igem/BBa_J11022/1
member: BBa_I13072
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member: BBa_I13404
member: BBa_I3521
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member: https://synbiohub.org/public/igem/BBa_J13204/1
member: https://synbiohub.org/public/igem/BBa_I13943/1
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member: BBa_I4002
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member: BBa_J13013
member: BBa_I2024
member: BBa_I9109

BBa_I9020
type: ComponentDefinition
persistent identity: BBa I9020
display id: BBa_I9020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: receiver (luxR) R1075.B0034.C0062.BOO15
creator: crackdots
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:08:11Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0010
component: BBa_B0034
component: BBa_B0012
component: BBa_C0062
component: BBa_R1075
sequence annotation: BBa_B0034
sequence annotation: BBa_C0062
sequence annotation: BBa_R1075
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
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BBa_B0010
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BBa_B0034
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BBa_B0012
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BBa_C0062
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BBa_R1075
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BBa_B0034
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BBa_B0010
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BBa_I9005
type: ComponentDefinition
persistent identity: BBa I9005
display id: BBa_I9005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: nor_input (tp901-cI) B0034.C0075
creator: crackdots
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:08:11Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_C0075
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_C0075
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BBa_C0075
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access: public
definition: https://synbiohub.org/public/igem/BBa_C0075/1

BBa_B0034
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BBa_B0034
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BBa_I15030
type: ComponentDefinition
persistent identity: BBa I15030
display id: BBa_I15030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3OC6HSL amplifier
creator: Jeff Tabor
created: 2005-05-01T11:00:00Z
modified: 2015-08-31T04:07:39Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _37_5_
m user id: 0
owner id: 88
owning group id: 5
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This device is a strong amplifier of 3OC6HSL. R0065 is a leaky promoter that produces enough LuxI to generate ample HSL to switch this device to a stable high expression state, the magnitude of which is dependent upon LuxR (or possibly lambda cI repressor) concentration.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0010
component: BBa_C0062
component: BBa_B0034
component: BBa_R0040
component: BBa_R0065
component: BBa_B0012
component: BBa_C0061
sequence annotation: BBa_B0034
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sequence annotation: BBa_B0012
sequence annotation: BBa_C0061
sequence annotation: BBa_B0010
sequence annotation: BBa_C0062
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BBa_B0010
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BBa_C0062
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version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_C0062/1

BBa_B0034
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display id: component1482756
version: 1
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definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
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BBa_R0040
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display id: component1482798
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definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_R0065
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definition: https://synbiohub.org/public/igem/BBa_R0065/1

BBa_B0012
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BBa_B0012
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BBa_B0010
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BBa_C0061
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BBa_B0034
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BBa_B0012
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BBa_R0040
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display id: annotation1482798
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BBa_I3112
type: ComponentDefinition
persistent identity: BBa I3112
display id: BBa_I3112
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Test: AraC trigger (I3102.E0422)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-28T12:00:00Z
modified: 2015-08-31T04:07:41Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Tests I3102 AraC trigger device by attaching TIPS output to CFP reporter.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_C0080
component: BBa_R0080
component: BBa_E0022
component: BBa_B0034
component: BBa_B0010
component: BBa_R1075
component: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_C0080
sequence annotation: BBa_R0080
sequence annotation: BBa_R1075
sequence annotation: BBa_E0022
sequence: https://synbiohub.org/public/igem/BBa_I3112_sequence/1

BBa_C0080
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display id: component968057
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_C0080/1

BBa_R0080
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display id: component968096
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_R0080/1

BBa_E0022
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display id: component968113
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_E0022/1

BBa_B0034
type: Component
persistent identity: component968047
display id: component968047
version: 1
top level: BBa_I3112
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
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display id: component968063
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R1075
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display id: component968042
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_R1075/1

BBa_B0010
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display id: component968121
version: 1
top level: BBa_I3112
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
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persistent identity: component968131
display id: component968131
version: 1
top level: BBa_I3112
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
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display id: component968101
version: 1
top level: BBa_I3112
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
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display id: component968073
version: 1
top level: BBa_I3112
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation968063
display id: annotation968063
version: 1
top level: BBa_I3112
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display id: BBa_I3521
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Test: Quorum threshold level and chemotaxis response, sender cells (I3600.I3520.I3500)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-28T12:00:00Z
modified: 2015-08-31T04:07:41Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Tests cellular density of asparate-secreting cells needed to induce quorum threshold response. Sender cells are nonmotile and have a trigger controlling the AspA attractant sender and CFP reporter indicating transcription of aspA. Receiver cells have a constitutive promoter controlling YFP reporter. Chemotaxis can be observed by the movement of the YFP-marked receiver cells. Quorum threshold is detected when the LacZ reporter is observed.
mutable notes: Sender cells are the nonchemotactic E. coli strain RP5232. Receiver cells are wildtype E. coli. This test combines the chemotaxis response test with the quorum threshold test.
owned by: james
owned by: myers
star: false
type: DnaRegion
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role: partType/Project
component: BBa_I3520
component: BBa_I3500
component: BBa_I3600
sequence annotation: BBa_I3600
sequence annotation: BBa_I3500
sequence annotation: BBa_I3520
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display id: BBa_I3701
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AraC input device for OR gate (B0034.C0080.B0015)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:07:41Z
discontinued: false
dominant: true
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This input device takes TIPS in as one of two OR gate inputs and produces AraC. AraC then acts on the araC promoter in I3700 to complete the OR gate.
owned by: james
owned by: myers
star: false
type: DnaRegion
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component: BBa_B0010
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type: ComponentDefinition
persistent identity: BBa I13019
display id: BBa_I13019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: QPI Test Construct Intermediate (Q04510.E0430)
creator: Barry Canton
created: 2004-07-13T11:00:00Z
modified: 2015-08-31T04:07:32Z
discontinued: false
dominant: true
experience: experience/None
group u list: _6_
m user id: 0
owner id: 159
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0012
component: BBa_B0034
component: BBa_C0051
component: BBa_B0010
component: BBa_E0030
component: BBa_R0051
sequence annotation: BBa_B0034
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sequence annotation: BBa_B0012
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sequence annotation: BBa_R0051
sequence: https://synbiohub.org/public/igem/BBa_I13019_sequence/1

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version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

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definition: https://synbiohub.org/public/igem/BBa_B0034/1

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definition: https://synbiohub.org/public/igem/BBa_C0051/1

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definition: https://synbiohub.org/public/igem/BBa_B0012/1

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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Test: Tar-EnvZ attractant receiver (I3300.E0422)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-28T12:00:00Z
modified: 2015-08-31T04:07:41Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Tests Tar-EnvZ attractant receiver device (ompC upstream version) by attaching TIPS output to CFP reporter.
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persistent identity: BBa I3001
display id: BBa_I3001
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: E. colibrator design 2, Polkadorks (I3410.I3200.E0422.I3510.I3401.I3411.B0015.I3100.I3701.I3300.I370
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:07:41Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: E. colibrator design 2 has an explicit OR gate before the switch via an araC OR gate device (I3701, I3700). Design 2 has no explicit activity during the switch OFF state, as illustrated by the terminator immediately following I3411 Lambda cI switch output device. This causes the OFF state to rely completely on cellular diffusion to reset the system.
mutable notes: The layout of E. colibrator (I3001) is designed to insulate the most sensitive devices, namely those in the switch that store state. The hk022cI switch output (ON) is insulated by appearing first. This output is then attached to the AspA attractant sender and the CFP reporter (I3410.I3200.E0422). The quorum receiver and its control of the lambda cI switch input (OFF) occur next (I3510.I3401) because leaky TIPS through this module will encourage the OFF state dicated by the downstream lambda cI switch output (I3411.B0015). Note that the OFF switch output simply ends at termination of the output TIPS (B0015) in this design. The rest of the switch inputs occur downstream because they do not store state and therefore do not need to be as insulated from leaky TIPS. Lac trigger controls the araC input device for the OR gate (I3100.I3701), and Tar-EnvZ receptor (I3300 upstream ompC version) controls the second OR input, embedded in the araC buffer device (I3300.I3700). The araC OR gate in turn outputs TIPS into the hk022cI switch input (I3400). Finally, the quorum sender device (I3500) is last because it is meant to be constitutively on at some basal level, so insulation is not required. Note: Depending on the efficacy of the OmpR promoter, this design can be implemented with any of I3300, I3301, I3302 (variations of Tar-EnvZ devices with different OmpR promoters). The default implementation uses I3300.
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modified: 2015-08-31T04:07:39Z
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group u list: _11_1_
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sample status: Not in stock
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bookmark: false
mutable description: R1075 was replaced with R0053, a medium-strength promoter. Trigger component added.
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BL oscillator (I12036 based) with IPTG kickstart
creator: endelman
created: 2004-09-02T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 145
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Modified BL oscillator based on I12036. It can be "reset" by adding IPTG which stimulates production of the A protein (lambda cI) if used in LacIq strain. The B protein is 434 cI. The YFP-AAV reporter tracks the dynamics of the A protein.
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modified: 2015-08-31T04:07:41Z
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description: Test: AspA attractant sender (I3100.I3200.B0015)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-28T12:00:00Z
modified: 2015-08-31T04:07:41Z
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mutable description: Tests Lac trigger ability to produce AspA. After trigger induction, the amount of aspartate secreted into the medium can be measured directly.
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created: 2004-01-28T12:00:00Z
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dominant: false
group u list: _1_
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description: Synchronator 2 w/ CFP under control of Lac (I4202.I13601)
creator: robertb
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modified: 2015-08-31T04:07:37Z
discontinued: true
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experience: experience/None
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display id: BBa_J06962
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lambda cI/LacI ts (mut 265) RS flip-flop with EYFP
creator: Yves Wang
created: 2005-07-31T11:00:00Z
modified: 2015-08-31T04:08:19Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _20_
m user id: 0
owner id: 340
owning group id: 20
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: RS flip-flop with LacI ts (mut 265) as the set and lambda cI as the reset. It will also take UV exposure as the set and heat (40??C) as the reset.
mutable notes: Sequenced. However, shows a 23 bp deletion in the LacI+pL regulated promoter (R0011).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0012
component: BBa_B0010
component: BBa_R0051
component: BBa_R0011
component: BBa_B0034
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component: BBa_E0030
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sequence annotation: BBa_R0051
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sequence annotation: BBa_B0012
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type: ComponentDefinition
persistent identity: BBa J06960
display id: BBa_J06960
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lambda cI/LacI RS flip-flop with EYFP
creator: Yves Wang
created: 2005-07-31T11:00:00Z
modified: 2015-08-31T04:08:19Z
discontinued: false
dominant: false
group u list: _20_
m user id: 0
owner id: 340
owning group id: 20
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: RS flip-flop with LacI as the set and lambda cI as the reset.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
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component: BBa_B0010
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component: BBa_C0012
component: BBa_C0051
component: BBa_R0011
component: BBa_E0030
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BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1599934
display id: annotation1599934
version: 1
top level: BBa_J06960
location: https://synbiohub.org/public/igem/BBa_J06960/annotation1599934/range1599934/1
component: BBa_R0011

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type: Range
persistent identity: range1599934
display id: range1599934
version: 1
direction: direction/forward
top level: BBa_J06960
start: 1374
end: 1427
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation1599953
display id: annotation1599953
version: 1
top level: BBa_J06960
location: https://synbiohub.org/public/igem/BBa_J06960/annotation1599953/range1599953/1
component: BBa_C0051

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type: Range
persistent identity: range1599953
display id: range1599953
version: 1
direction: direction/forward
top level: BBa_J06960
start: 1455
end: 2204
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1599992
display id: annotation1599992
version: 1
top level: BBa_J06960
location: https://synbiohub.org/public/igem/BBa_J06960/annotation1599992/range1599992/1
component: BBa_B0034

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type: Range
persistent identity: range1599992
display id: range1599992
version: 1
direction: direction/forward
top level: BBa_J06960
start: 2432
end: 2443
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1599959
display id: annotation1599959
version: 1
top level: BBa_J06960
location: https://synbiohub.org/public/igem/BBa_J06960/annotation1599959/range1599959/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J06960/annotation1599959/range1599959/1
type: Range
persistent identity: range1599959
display id: range1599959
version: 1
direction: direction/forward
top level: BBa_J06960
start: 2238
end: 2317
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation1599994
display id: annotation1599994
version: 1
top level: BBa_J06960
location: https://synbiohub.org/public/igem/BBa_J06960/annotation1599994/range1599994/1
component: BBa_E0030

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type: Range
persistent identity: range1599994
display id: range1599994
version: 1
direction: direction/forward
top level: BBa_J06960
start: 2450
end: 3172
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation1599885
display id: annotation1599885
version: 1
top level: BBa_J06960
location: https://synbiohub.org/public/igem/BBa_J06960/annotation1599885/range1599885/1
component: BBa_R0051

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type: Range
persistent identity: range1599885
display id: range1599885
version: 1
direction: direction/forward
top level: BBa_J06960
start: 1
end: 49
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation1599984
display id: annotation1599984
version: 1
top level: BBa_J06960
location: https://synbiohub.org/public/igem/BBa_J06960/annotation1599984/range1599984/1
component: BBa_R0051

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type: Range
persistent identity: range1599984
display id: range1599984
version: 1
direction: direction/forward
top level: BBa_J06960
start: 2375
end: 2423
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1600009
display id: annotation1600009
version: 1
top level: BBa_J06960
location: https://synbiohub.org/public/igem/BBa_J06960/annotation1600009/range1600009/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J06960/annotation1600009/range1600009/1
type: Range
persistent identity: range1600009
display id: range1600009
version: 1
direction: direction/forward
top level: BBa_J06960
start: 3269
end: 3309
orientation: inline

BBa_I13901
type: ComponentDefinition
persistent identity: BBa I13901
display id: BBa_I13901
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Synchronized Brickilator Test Construct (I13201.Q04400.I0461)
creator: robertb
created: 2004-08-03T11:00:00Z
modified: 2015-08-31T04:07:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 159
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: LuxR/Plux + tet Inverter + LuxI
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_R0040
component: BBa_C0040
component: BBa_C0061
component: BBa_B0010
component: BBa_I13201
component: BBa_B0034
component: BBa_B0012
sequence annotation: BBa_I13201
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_C0040
sequence annotation: BBa_C0061
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_I13901_sequence/1

BBa_R0040
type: Component
persistent identity: component1025303
display id: component1025303
version: 1
top level: BBa_I13901
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_C0040
type: Component
persistent identity: component1025272
display id: component1025272
version: 1
top level: BBa_I13901
access: public
definition: https://synbiohub.org/public/igem/BBa_C0040/1

BBa_C0061
type: Component
persistent identity: component1025321
display id: component1025321
version: 1
top level: BBa_I13901
access: public
definition: https://synbiohub.org/public/igem/BBa_C0061/1

BBa_B0010
type: Component
persistent identity: component1025278
display id: component1025278
version: 1
top level: BBa_I13901
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
type: Component
persistent identity: component1025328
display id: component1025328
version: 1
top level: BBa_I13901
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I13201
type: Component
persistent identity: component1025257
display id: component1025257
version: 1
top level: BBa_I13901
access: public
definition: https://synbiohub.org/public/igem/BBa_I13201/1

BBa_B0034
type: Component
persistent identity: component1025262
display id: component1025262
version: 1
top level: BBa_I13901
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component1025338
display id: component1025338
version: 1
top level: BBa_I13901
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
type: Component
persistent identity: component1025288
display id: component1025288
version: 1
top level: BBa_I13901
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component1025311
display id: component1025311
version: 1
top level: BBa_I13901
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I13201
type: SequenceAnnotation
persistent identity: annotation1025257
display id: annotation1025257
version: 1
top level: BBa_I13901
location: https://synbiohub.org/public/igem/BBa_I13901/annotation1025257/range1025257/1
component: BBa_I13201

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type: Range
persistent identity: range1025257
display id: range1025257
version: 1
direction: direction/forward
top level: BBa_I13901
start: 1
end: 1023
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1025328
display id: annotation1025328
version: 1
top level: BBa_I13901
location: https://synbiohub.org/public/igem/BBa_I13901/annotation1025328/range1025328/1
component: BBa_B0010

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type: Range
persistent identity: range1025328
display id: range1025328
version: 1
direction: direction/forward
top level: BBa_I13901
start: 2611
end: 2690
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1025262
display id: annotation1025262
version: 1
top level: BBa_I13901
location: https://synbiohub.org/public/igem/BBa_I13901/annotation1025262/range1025262/1
component: BBa_B0034

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type: Range
persistent identity: range1025262
display id: range1025262
version: 1
direction: direction/forward
top level: BBa_I13901
start: 1032
end: 1043
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1025338
display id: annotation1025338
version: 1
top level: BBa_I13901
location: https://synbiohub.org/public/igem/BBa_I13901/annotation1025338/range1025338/1
component: BBa_B0012

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type: Range
persistent identity: range1025338
display id: range1025338
version: 1
direction: direction/forward
top level: BBa_I13901
start: 2699
end: 2739
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation1025272
display id: annotation1025272
version: 1
top level: BBa_I13901
location: https://synbiohub.org/public/igem/BBa_I13901/annotation1025272/range1025272/1
component: BBa_C0040

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type: Range
persistent identity: range1025272
display id: range1025272
version: 1
direction: direction/forward
top level: BBa_I13901
start: 1050
end: 1709
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1025278
display id: annotation1025278
version: 1
top level: BBa_I13901
location: https://synbiohub.org/public/igem/BBa_I13901/annotation1025278/range1025278/1
component: BBa_B0010

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type: Range
persistent identity: range1025278
display id: range1025278
version: 1
direction: direction/forward
top level: BBa_I13901
start: 1743
end: 1822
orientation: inline

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation1025321
display id: annotation1025321
version: 1
top level: BBa_I13901
location: https://synbiohub.org/public/igem/BBa_I13901/annotation1025321/range1025321/1
component: BBa_C0061

https://synbiohub.org/public/igem/BBa_I13901/annotation1025321/range1025321/1
type: Range
persistent identity: range1025321
display id: range1025321
version: 1
direction: direction/forward
top level: BBa_I13901
start: 1960
end: 2577
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1025288
display id: annotation1025288
version: 1
top level: BBa_I13901
location: https://synbiohub.org/public/igem/BBa_I13901/annotation1025288/range1025288/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13901/annotation1025288/range1025288/1
type: Range
persistent identity: range1025288
display id: range1025288
version: 1
direction: direction/forward
top level: BBa_I13901
start: 1831
end: 1871
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1025303
display id: annotation1025303
version: 1
top level: BBa_I13901
location: https://synbiohub.org/public/igem/BBa_I13901/annotation1025303/range1025303/1
component: BBa_R0040

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type: Range
persistent identity: range1025303
display id: range1025303
version: 1
direction: direction/forward
top level: BBa_I13901
start: 1880
end: 1933
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1025311
display id: annotation1025311
version: 1
top level: BBa_I13901
location: https://synbiohub.org/public/igem/BBa_I13901/annotation1025311/range1025311/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13901/annotation1025311/range1025311/1
type: Range
persistent identity: range1025311
display id: range1025311
version: 1
direction: direction/forward
top level: BBa_I13901
start: 1942
end: 1953
orientation: inline

BBa_I12000
type: ComponentDefinition
persistent identity: BBa I12000
display id: BBa_I12000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Barkai-Leibler design experiment Part A (Lambda cI)
creator: mcnamara
created: 2004-07-11T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 147
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This forms the activator of a Barkai-Leibler oscillator.
mutable notes: This part to work in conjunction with BBa_I12002. This part creates one of the proteins involved in the Modified Barkai-Leibler relaxation oscillator design (activator protein: lambda cI). In the presence of Lambda cI, more Lambda cI will be produced (positively autoregulated). However, if there is 434 cI repressor present in the system, the production of Lambda cI should be repressed. This was implemented in the subpart BBa_I12006, which represents the Operating Regions for the promoter. The fact that the OR-3 site was replaced with the OR-1 site of 434 cI repressor theoretically should mean that when 434 cI binds in, the production of the Lambda cI protein should be blocked.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_I12006
component: BBa_B0034
component: BBa_C0051
component: BBa_B0015
component: BBa_B0025
sequence annotation: BBa_I12006
sequence annotation: BBa_B0025
sequence annotation: BBa_B0034
sequence annotation: BBa_C0051
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_I12000_sequence/1

BBa_I12006
type: Component
persistent identity: component2223457
display id: component2223457
version: 1
top level: BBa_I12000
access: public
definition: https://synbiohub.org/public/igem/BBa_I12006/1

BBa_B0034
type: Component
persistent identity: component2223459
display id: component2223459
version: 1
top level: BBa_I12000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0051
type: Component
persistent identity: component2223463
display id: component2223463
version: 1
top level: BBa_I12000
access: public
definition: https://synbiohub.org/public/igem/BBa_C0051/1

BBa_B0015
type: Component
persistent identity: component2223470
display id: component2223470
version: 1
top level: BBa_I12000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0025
type: Component
persistent identity: component2223451
display id: component2223451
version: 1
top level: BBa_I12000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0025/1

BBa_I12006
type: SequenceAnnotation
persistent identity: annotation2223457
display id: annotation2223457
version: 1
top level: BBa_I12000
location: https://synbiohub.org/public/igem/BBa_I12000/annotation2223457/range2223457/1
component: BBa_I12006

https://synbiohub.org/public/igem/BBa_I12000/annotation2223457/range2223457/1
type: Range
persistent identity: range2223457
display id: range2223457
version: 1
direction: direction/reverse
top level: BBa_I12000
start: 138
end: 219
orientation: inline

BBa_B0025
type: SequenceAnnotation
persistent identity: annotation2223451
display id: annotation2223451
version: 1
top level: BBa_I12000
location: https://synbiohub.org/public/igem/BBa_I12000/annotation2223451/range2223451/1
component: BBa_B0025

https://synbiohub.org/public/igem/BBa_I12000/annotation2223451/range2223451/1
type: Range
persistent identity: range2223451
display id: range2223451
version: 1
direction: direction/reverse
top level: BBa_I12000
start: 1
end: 129
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2223459
display id: annotation2223459
version: 1
top level: BBa_I12000
location: https://synbiohub.org/public/igem/BBa_I12000/annotation2223459/range2223459/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I12000/annotation2223459/range2223459/1
type: Range
persistent identity: range2223459
display id: range2223459
version: 1
direction: direction/reverse
top level: BBa_I12000
start: 228
end: 239
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation2223463
display id: annotation2223463
version: 1
top level: BBa_I12000
location: https://synbiohub.org/public/igem/BBa_I12000/annotation2223463/range2223463/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_I12000/annotation2223463/range2223463/1
type: Range
persistent identity: range2223463
display id: range2223463
version: 1
direction: direction/reverse
top level: BBa_I12000
start: 246
end: 1020
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2223470
display id: annotation2223470
version: 1
top level: BBa_I12000
location: https://synbiohub.org/public/igem/BBa_I12000/annotation2223470/range2223470/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_I12000/annotation2223470/range2223470/1
type: Range
persistent identity: range2223470
display id: range2223470
version: 1
direction: direction/reverse
top level: BBa_I12000
start: 1029
end: 1157
orientation: inline

BBa_I13033
type: ComponentDefinition
persistent identity: BBa I13033
display id: BBa_I13033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: luxR QPI intermediate
creator: jenmitch
created: 2004-07-14T11:00:00Z
modified: 2015-08-31T04:07:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 159
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_R0062
component: BBa_B0010
component: BBa_B0012
sequence annotation: BBa_R0062
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_I13033_sequence/1

BBa_R0062
type: Component
persistent identity: component941333
display id: component941333
version: 1
top level: BBa_I13033
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_B0010
type: Component
persistent identity: component941308
display id: component941308
version: 1
top level: BBa_I13033
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component941318
display id: component941318
version: 1
top level: BBa_I13033
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation941333
display id: annotation941333
version: 1
top level: BBa_I13033
location: https://synbiohub.org/public/igem/BBa_I13033/annotation941333/range941333/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_I13033/annotation941333/range941333/1
type: Range
persistent identity: range941333
display id: range941333
version: 1
direction: direction/forward
top level: BBa_I13033
start: 138
end: 192
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation941318
display id: annotation941318
version: 1
top level: BBa_I13033
location: https://synbiohub.org/public/igem/BBa_I13033/annotation941318/range941318/1
component: BBa_B0012

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type: Range
persistent identity: range941318
display id: range941318
version: 1
direction: direction/forward
top level: BBa_I13033
start: 89
end: 129
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation941308
display id: annotation941308
version: 1
top level: BBa_I13033
location: https://synbiohub.org/public/igem/BBa_I13033/annotation941308/range941308/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13033/annotation941308/range941308/1
type: Range
persistent identity: range941308
display id: range941308
version: 1
direction: direction/forward
top level: BBa_I13033
start: 1
end: 80
orientation: inline

BBa_I8030
type: ComponentDefinition
persistent identity: BBa I8030
display id: BBa_I8030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Booperator aiia test: degradation of HSL "B" by aiia
creator: Srini Devadas, David Gray, Ronny Krashinsky, Debra Lin, and Chris Zheng Liu
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:08:11Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0012
component: BBa_B0034
component: BBa_R0071
component: BBa_C0060
component: BBa_B0010
component: BBa_C0071
component: BBa_C0040
component: BBa_E0032
component: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_C0060
sequence annotation: BBa_R0040
sequence annotation: BBa_R0071
sequence annotation: BBa_C0040
sequence annotation: BBa_E0032
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_C0071
sequence: https://synbiohub.org/public/igem/BBa_I8030_sequence/1

BBa_B0012
type: Component
persistent identity: component976517
display id: component976517
version: 1
top level: BBa_I8030
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component976468
display id: component976468
version: 1
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description: Booperator: Lux/Rhl, monocistronic
creator: Srini Devadas, David Gray, Ronny Krashinsky, Debra Lin, and Chris Zheng Liu
created: 2004-01-26T12:00:00Z
modified: 2015-08-31T04:08:10Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0012
component: BBa_B0010
component: BBa_R0051
component: BBa_B0034
component: BBa_C0052
component: BBa_C0061
component: BBa_B0032
component: BBa_R0071
component: BBa_C0050
component: BBa_R0040
component: BBa_C0071
component: BBa_R0052
component: BBa_E0022
component: BBa_C0062
component: BBa_C0070
component: BBa_C0040
component: BBa_C0051
component: BBa_R0062
component: BBa_R0050

BBa_B0012
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display id: component1012296
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
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display id: component1012709
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definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
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display id: component1012853
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definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0051
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display id: component1012363
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definition: https://synbiohub.org/public/igem/BBa_R0051/1

BBa_B0034
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display id: component1012470
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BBa_B0012
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display id: component1012595
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definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
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display id: component1012387
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definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
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display id: component1012500
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definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
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BBa_B0010
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BBa_B0012
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display id: component1012758
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definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0052
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display id: component1012578
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definition: https://synbiohub.org/public/igem/BBa_C0052/1

BBa_B0034
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definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
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display id: component1012537
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BBa_C0061
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BBa_B0010
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BBa_B0032
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BBa_B0034
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BBa_R0071
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BBa_B0012
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BBa_C0050
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BBa_B0010
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BBa_R0040
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BBa_B0010
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BBa_C0071
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BBa_B0010
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BBa_B0010
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BBa_B0034
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BBa_C0061
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BBa_R0052
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BBa_B0010
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BBa_R0040
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BBa_E0022
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BBa_B0012
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BBa_B0010
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BBa_R0051
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BBa_B0012
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BBa_B0034
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BBa_B0034
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BBa_C0062
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BBa_C0070
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BBa_B0034
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BBa_B0012
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display id: component1012447
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0040
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display id: component1012724
version: 1
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BBa_B0012
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display id: component1012397
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BBa_B0012
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BBa_C0040
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BBa_B0034
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BBa_R0071
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definition: https://synbiohub.org/public/igem/BBa_R0071/1

BBa_B0034
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display id: component1012568
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BBa_B0034
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BBa_C0051
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definition: https://synbiohub.org/public/igem/BBa_C0051/1

BBa_R0062
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definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_R0050
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BBa_B0010
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BBa_C0051
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definition: https://synbiohub.org/public/igem/BBa_C0051/1

BBa_B0012
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BBa_E0032
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BBa_R0071
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definition: https://synbiohub.org/public/igem/BBa_R0071/1

BBa_R0062
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BBa_B0012
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BBa_R0071
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BBa_C0070
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BBa_B0010
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BBa_B0010
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BBa_B0012
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BBa_R0040
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BBa_R0062
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BBa_E0022
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BBa_R0051
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BBa_I12060
type: ComponentDefinition
persistent identity: BBa I12060
display id: BBa_I12060
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BL oscillator (I12040 based) with IPTG kickstart
creator: endelman
created: 2004-09-02T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 145
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Modified BL oscillator based on I12040. It can be "reset" by adding IPTG which stimulates production of the A protein (lambda cI) if used in LacIq strain. The B protein is 434 cI. The YFP-AAV reporter tracks the dynamics of the A protein.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_E0434
component: BBa_I12024
component: BBa_I12054
component: BBa_I12057
sequence annotation: BBa_I12024
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persistent identity: BBa I2022
display id: BBa_I2022
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cI857 Test (I0500.B0034.E0022.C0081.B0015.R0051.E0032)
creator: Alvin Carter Powers, Yong-Su Jin (Fighting Darwins)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:39Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: test of cI-857: input TIPs with I0500, E0022 reporter indicates amount of TIPs, cI-857 is expressed, represses R0051, reporter E0032 indicates TIPs leakage
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_E0432
component: BBa_I2012
component: BBa_I0500
component: BBa_E0422
sequence annotation: BBa_I2012
sequence annotation: BBa_E0432
sequence annotation: BBa_E0422
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version: 1
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type: ComponentDefinition
persistent identity: BBa J13100
display id: BBa_J13100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pOmpR dependent dual repressor device for I15030 and J13030
creator: Laura Lavery
created: 2005-06-21T11:00:00Z
modified: 2015-08-31T04:08:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _37_
m user id: 0
owner id: 327
owning group id: 37
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: In the presence of phosphorylated ompR the proteins CI and TetR will be coexpressed from a single mRNA. These two proteins repress the HSL and PAI amplifiers I15030 and J13030. When using Cph1/EnvZ (I15010) as the OmpR regulator, these repressors will be expressed in the dark.
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owned by: myers
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role: partType/Project
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component: BBa_B0034
component: BBa_B0012
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type: ComponentDefinition
persistent identity: BBa I8100
display id: BBa_I8100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RhlR and LuxR constitutive generation (using TetR)
creator: Ronny Krashinsky
created: 2004-01-26T12:00:00Z
modified: 2015-08-31T04:08:11Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Produces RhlR and LuxR constitutively under control of a TetR self-repressor.
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owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0034
component: BBa_B0010
component: BBa_C0062
component: BBa_B0012
component: BBa_R0040
component: BBa_C0040
component: BBa_C0071
sequence annotation: BBa_B0034
sequence annotation: BBa_C0062
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definition: https://synbiohub.org/public/igem/BBa_B0034/1

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definition: https://synbiohub.org/public/igem/BBa_B0010/1

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version: 1
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definition: https://synbiohub.org/public/igem/BBa_C0062/1

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display id: component980817
version: 1
top level: BBa_I8100
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

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version: 1
top level: BBa_I8100
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definition: https://synbiohub.org/public/igem/BBa_B0012/1

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BBa_R0040
type: SequenceAnnotation
persistent identity: annotation980776
display id: annotation980776
version: 1
top level: BBa_I8100
location: https://synbiohub.org/public/igem/BBa_I8100/annotation980776/range980776/1
component: BBa_R0040

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type: Range
persistent identity: range980776
display id: range980776
version: 1
direction: direction/forward
top level: BBa_I8100
start: 1923
end: 1976
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation980735
display id: annotation980735
version: 1
top level: BBa_I8100
location: https://synbiohub.org/public/igem/BBa_I8100/annotation980735/range980735/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I8100/annotation980735/range980735/1
type: Range
persistent identity: range980735
display id: range980735
version: 1
direction: direction/forward
top level: BBa_I8100
start: 973
end: 984
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation980678
display id: annotation980678
version: 1
top level: BBa_I8100
location: https://synbiohub.org/public/igem/BBa_I8100/annotation980678/range980678/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I8100/annotation980678/range980678/1
type: Range
persistent identity: range980678
display id: range980678
version: 1
direction: direction/forward
top level: BBa_I8100
start: 1
end: 54
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation980807
display id: annotation980807
version: 1
top level: BBa_I8100
location: https://synbiohub.org/public/igem/BBa_I8100/annotation980807/range980807/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I8100/annotation980807/range980807/1
type: Range
persistent identity: range980807
display id: range980807
version: 1
direction: direction/forward
top level: BBa_I8100
start: 2792
end: 2871
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation980702
display id: annotation980702
version: 1
top level: BBa_I8100
location: https://synbiohub.org/public/igem/BBa_I8100/annotation980702/range980702/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I8100/annotation980702/range980702/1
type: Range
persistent identity: range980702
display id: range980702
version: 1
direction: direction/forward
top level: BBa_I8100
start: 774
end: 853
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation980727
display id: annotation980727
version: 1
top level: BBa_I8100
location: https://synbiohub.org/public/igem/BBa_I8100/annotation980727/range980727/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I8100/annotation980727/range980727/1
type: Range
persistent identity: range980727
display id: range980727
version: 1
direction: direction/forward
top level: BBa_I8100
start: 911
end: 964
orientation: inline

BBa_I13027
type: ComponentDefinition
persistent identity: BBa I13027
display id: BBa_I13027
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: penI QPI intermediate
creator: jenmitch
created: 2004-07-14T11:00:00Z
modified: 2015-08-31T04:07:32Z
discontinued: false
dominant: true
experience: experience/None
group u list: _6_
m user id: 0
owner id: 159
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0034
component: BBa_C0074
sequence annotation: BBa_C0074
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I13027_sequence/1

BBa_B0034
type: Component
persistent identity: component939448
display id: component939448
version: 1
top level: BBa_I13027
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0074
type: Component
persistent identity: component939458
display id: component939458
version: 1
top level: BBa_I13027
access: public
definition: https://synbiohub.org/public/igem/BBa_C0074/1

BBa_C0074
type: SequenceAnnotation
persistent identity: annotation939458
display id: annotation939458
version: 1
top level: BBa_I13027
location: https://synbiohub.org/public/igem/BBa_I13027/annotation939458/range939458/1
component: BBa_C0074

https://synbiohub.org/public/igem/BBa_I13027/annotation939458/range939458/1
type: Range
persistent identity: range939458
display id: range939458
version: 1
direction: direction/forward
top level: BBa_I13027
start: 19
end: 441
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation939448
display id: annotation939448
version: 1
top level: BBa_I13027
location: https://synbiohub.org/public/igem/BBa_I13027/annotation939448/range939448/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13027/annotation939448/range939448/1
type: Range
persistent identity: range939448
display id: range939448
version: 1
direction: direction/forward
top level: BBa_I13027
start: 1
end: 12
orientation: inline

BBa_I12042
type: ComponentDefinition
persistent identity: BBa I12042
display id: BBa_I12042
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BL oscillator, no cooperativity, reporter protein, kickstart
creator: Hans
created: 2004-08-19T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 103
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is a Barkai-Leibler oscillator with no cooperativity for the repressor (one 434 cI OR site). A reporter protein (EYFP) and a kickstart is included.
mutable notes: There is no cooperativity for 434 cI in this oscillator. A protein EYFP (with degradation tag) coding region is placed directly after the lambda cI coding region in order to keep track of the lambda cI levels. This part also includes a "kickstart" that will yield lambda cI if ATC is added.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_I12038
component: BBa_I12002
component: BBa_I12043
sequence annotation: BBa_I12043
sequence annotation: BBa_I12002
sequence annotation: BBa_I12038
sequence: https://synbiohub.org/public/igem/BBa_I12042_sequence/1

BBa_I12038
type: Component
persistent identity: component2238886
display id: component2238886
version: 1
top level: BBa_I12042
access: public
definition: BBa_I12038

BBa_I12002
type: Component
persistent identity: component2238946
display id: component2238946
version: 1
top level: BBa_I12042
access: public
definition: https://synbiohub.org/public/igem/BBa_I12002/1

BBa_I12043
type: Component
persistent identity: component2238925
display id: component2238925
version: 1
top level: BBa_I12042
access: public
definition: https://synbiohub.org/public/igem/BBa_I12043/1

BBa_I12043
type: SequenceAnnotation
persistent identity: annotation2238925
display id: annotation2238925
version: 1
top level: BBa_I12042
location: https://synbiohub.org/public/igem/BBa_I12042/annotation2238925/range2238925/1
component: BBa_I12043

https://synbiohub.org/public/igem/BBa_I12042/annotation2238925/range2238925/1
type: Range
persistent identity: range2238925
display id: range2238925
version: 1
direction: direction/reverse
top level: BBa_I12042
start: 1001
end: 3102
orientation: inline

BBa_I12002
type: SequenceAnnotation
persistent identity: annotation2238946
display id: annotation2238946
version: 1
top level: BBa_I12042
location: https://synbiohub.org/public/igem/BBa_I12042/annotation2238946/range2238946/1
component: BBa_I12002

https://synbiohub.org/public/igem/BBa_I12042/annotation2238946/range2238946/1
type: Range
persistent identity: range2238946
display id: range2238946
version: 1
direction: direction/reverse
top level: BBa_I12042
start: 3111
end: 4128
orientation: inline

BBa_I12038
type: SequenceAnnotation
persistent identity: annotation2238886
display id: annotation2238886
version: 1
top level: BBa_I12042
location: https://synbiohub.org/public/igem/BBa_I12042/annotation2238886/range2238886/1
component: BBa_I12038

https://synbiohub.org/public/igem/BBa_I12042/annotation2238886/range2238886/1
type: Range
persistent identity: range2238886
display id: range2238886
version: 1
direction: direction/reverse
top level: BBa_I12042
start: 1
end: 992
orientation: inline

BBa_I12025
type: ComponentDefinition
persistent identity: BBa I12025
display id: BBa_I12025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Test construct for I12007
creator: endelman
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 145
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: When used in LacIq strain, addition of IPTG should increase YFP fluorescence if I12007 is working.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_E0430
component: BBa_I12024
component: BBa_I12007
sequence annotation: BBa_I12007
sequence annotation: BBa_I12024
sequence annotation: BBa_E0430
sequence: https://synbiohub.org/public/igem/BBa_I12025_sequence/1

BBa_E0430
type: Component
persistent identity: component2229489
display id: component2229489
version: 1
top level: BBa_I12025
access: public
definition: https://synbiohub.org/public/igem/BBa_E0430/1

BBa_I12024
type: Component
persistent identity: component2229473
display id: component2229473
version: 1
top level: BBa_I12025
access: public
definition: https://synbiohub.org/public/igem/BBa_I12024/1

BBa_I12007
type: Component
persistent identity: component2229479
display id: component2229479
version: 1
top level: BBa_I12025
access: public
definition: https://synbiohub.org/public/igem/BBa_I12007/1

BBa_I12007
type: SequenceAnnotation
persistent identity: annotation2229479
display id: annotation2229479
version: 1
top level: BBa_I12025
location: https://synbiohub.org/public/igem/BBa_I12025/annotation2229479/range2229479/1
component: BBa_I12007

https://synbiohub.org/public/igem/BBa_I12025/annotation2229479/range2229479/1
type: Range
persistent identity: range2229479
display id: range2229479
version: 1
direction: direction/reverse
top level: BBa_I12025
start: 1002
end: 1083
orientation: inline

BBa_I12024
type: SequenceAnnotation
persistent identity: annotation2229473
display id: annotation2229473
version: 1
top level: BBa_I12025
location: https://synbiohub.org/public/igem/BBa_I12025/annotation2229473/range2229473/1
component: BBa_I12024

https://synbiohub.org/public/igem/BBa_I12025/annotation2229473/range2229473/1
type: Range
persistent identity: range2229473
display id: range2229473
version: 1
direction: direction/reverse
top level: BBa_I12025
start: 1
end: 993
orientation: inline

BBa_E0430
type: SequenceAnnotation
persistent identity: annotation2229489
display id: annotation2229489
version: 1
top level: BBa_I12025
location: https://synbiohub.org/public/igem/BBa_I12025/annotation2229489/range2229489/1
component: BBa_E0430

https://synbiohub.org/public/igem/BBa_I12025/annotation2229489/range2229489/1
type: Range
persistent identity: range2229489
display id: range2229489
version: 1
direction: direction/reverse
top level: BBa_I12025
start: 1092
end: 1969
orientation: inline

BBa_I13030
type: ComponentDefinition
persistent identity: BBa I13030
display id: BBa_I13030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mnt (weak) QPI intermediate
creator: jenmitch
created: 2004-07-14T11:00:00Z
modified: 2015-08-31T04:07:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 159
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0010
component: BBa_B0012
component: BBa_R0073
sequence annotation: BBa_R0073
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_I13030_sequence/1

BBa_B0010
type: Component
persistent identity: component941212
display id: component941212
version: 1
top level: BBa_I13030
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component941222
display id: component941222
version: 1
top level: BBa_I13030
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0073
type: Component
persistent identity: component941239
display id: component941239
version: 1
top level: BBa_I13030
access: public
definition: https://synbiohub.org/public/igem/BBa_R0073/1

BBa_R0073
type: SequenceAnnotation
persistent identity: annotation941239
display id: annotation941239
version: 1
top level: BBa_I13030
location: https://synbiohub.org/public/igem/BBa_I13030/annotation941239/range941239/1
component: BBa_R0073

https://synbiohub.org/public/igem/BBa_I13030/annotation941239/range941239/1
type: Range
persistent identity: range941239
display id: range941239
version: 1
direction: direction/forward
top level: BBa_I13030
start: 138
end: 204
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation941222
display id: annotation941222
version: 1
top level: BBa_I13030
location: https://synbiohub.org/public/igem/BBa_I13030/annotation941222/range941222/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13030/annotation941222/range941222/1
type: Range
persistent identity: range941222
display id: range941222
version: 1
direction: direction/forward
top level: BBa_I13030
start: 89
end: 129
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation941212
display id: annotation941212
version: 1
top level: BBa_I13030
location: https://synbiohub.org/public/igem/BBa_I13030/annotation941212/range941212/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13030/annotation941212/range941212/1
type: Range
persistent identity: range941212
display id: range941212
version: 1
direction: direction/forward
top level: BBa_I13030
start: 1
end: 80
orientation: inline

BBa_I13913
type: ComponentDefinition
persistent identity: BBa I13913
display id: BBa_I13913
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AiiA (-LVA) Tri-part (B0032.C0160.B0015)
creator: robertb
created: 2004-08-03T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 159
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Medium RBS + AiiA (-LVA) + Term
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0010
component: BBa_B0032
component: BBa_B0012
component: BBa_C0160
sequence annotation: BBa_C0160
sequence annotation: BBa_B0032
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I13913_sequence/1

BBa_B0010
type: Component
persistent identity: component1025584
display id: component1025584
version: 1
top level: BBa_I13913
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0032
type: Component
persistent identity: component1025576
display id: component1025576
version: 1
top level: BBa_I13913
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_B0012
type: Component
persistent identity: component1025594
display id: component1025594
version: 1
top level: BBa_I13913
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0160
type: Component
persistent identity: component1025579
display id: component1025579
version: 1
top level: BBa_I13913
access: public
definition: https://synbiohub.org/public/igem/BBa_C0160/1

BBa_C0160
type: SequenceAnnotation
persistent identity: annotation1025579
display id: annotation1025579
version: 1
top level: BBa_I13913
location: https://synbiohub.org/public/igem/BBa_I13913/annotation1025579/range1025579/1
component: BBa_C0160

https://synbiohub.org/public/igem/BBa_I13913/annotation1025579/range1025579/1
type: Range
persistent identity: range1025579
display id: range1025579
version: 1
direction: direction/forward
top level: BBa_I13913
start: 20
end: 775
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1025576
display id: annotation1025576
version: 1
top level: BBa_I13913
location: https://synbiohub.org/public/igem/BBa_I13913/annotation1025576/range1025576/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_I13913/annotation1025576/range1025576/1
type: Range
persistent identity: range1025576
display id: range1025576
version: 1
direction: direction/forward
top level: BBa_I13913
start: 1
end: 13
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1025584
display id: annotation1025584
version: 1
top level: BBa_I13913
location: https://synbiohub.org/public/igem/BBa_I13913/annotation1025584/range1025584/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13913/annotation1025584/range1025584/1
type: Range
persistent identity: range1025584
display id: range1025584
version: 1
direction: direction/forward
top level: BBa_I13913
start: 784
end: 863
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1025594
display id: annotation1025594
version: 1
top level: BBa_I13913
location: https://synbiohub.org/public/igem/BBa_I13913/annotation1025594/range1025594/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13913/annotation1025594/range1025594/1
type: Range
persistent identity: range1025594
display id: range1025594
version: 1
direction: direction/forward
top level: BBa_I13913
start: 872
end: 912
orientation: inline

BBa_I3000
type: ComponentDefinition
persistent identity: BBa I3000
display id: BBa_I3000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: E. colibrator design 1, Polkadorks (I3410.I3200.E0422.I3510.I3401.I3411.B0015.I3100.I3400.I3300.I340
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-28T12:00:00Z
modified: 2015-08-31T04:07:41Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: E. colibrator design 1 has the OR gate embedded within the switch via a double instance of I3400 Hk022cI switch input device. Design 1 also has no explicit activity during the switch OFF state, as illustrated by the terminator immediately following I3411 Lambda cI switch output device. This causes the OFF state to rely completely on cellular diffusion to reset the system.
mutable notes: The layout of E. colibrator (I3000) is designed to insulate the most sensitive devices, namely those in the switch that store state. The hk022cI switch output (ON) is insulated by appearing first. This output is then attached to the AspA attractant sender and the CFP reporter (I3410.I3200.E0422). The quorum receiver and its control of the lambda cI switch input (OFF) occur next (I3510.I3401) because leaky TIPS through this module will encourage the OFF state dicated by the downstream lambda cI switch output (I3411.B0015). Note that the OFF switch output simply ends at termination of the output TIPS (B0015) in this design. The rest of the switch inputs occur downstream because they do not store state and therefore do not need to be as insulated from leaky TIPS. Lac trigger controls one hk022cI input (I3100.I3400), and Tar-EnvZ receptor (I3300 upstream ompC version) controls the other (I3300.I3400). The dual control forms the built-in OR gate in one of the switch inputs. Finally, the quorum sender device (I3500) is last because it is meant to be constitutively on at some basal level, so insulation is not required. Note: Depending on the efficacy of the OmpR promoter, this design can be implemented with any of I3300, I3301, I3302 (variations of Tar-EnvZ devices with different OmpR promoters). The default implementation uses I3300.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0010
component: BBa_R0063
component: BBa_C0082
component: BBa_B0034
component: BBa_B0012
component: BBa_C0083
component: BBa_R0010
component: BBa_C0050
component: BBa_R0082
component: BBa_C0062
component: BBa_E0022
component: BBa_R1075
component: BBa_C0061
component: BBa_R0051
component: BBa_C0051
component: BBa_R0050
component: BBa_R0062
component: BBa_C0012
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_C0082

BBa_B0010
type: Component
persistent identity: component1009151
display id: component1009151
version: 1
top level: BBa_I3000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
type: Component
persistent identity: component1009461
display id: component1009461
version: 1
top level: BBa_I3000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
type: Component
persistent identity: component1009353
display id: component1009353
version: 1
top level: BBa_I3000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0063
type: Component
persistent identity: component1009070
display id: component1009070
version: 1
top level: BBa_I3000
access: public
definition: https://synbiohub.org/public/igem/BBa_R0063/1

BBa_B0010
type: Component
persistent identity: component1009043
display id: component1009043
version: 1
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start: 81
end: 830
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation978349
display id: annotation978349
version: 1
top level: BBa_I5400
location: https://synbiohub.org/public/igem/BBa_I5400/annotation978349/range978349/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I5400/annotation978349/range978349/1
type: Range
persistent identity: range978349
display id: range978349
version: 1
direction: direction/forward
top level: BBa_I5400
start: 1024
end: 1035
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation978408
display id: annotation978408
version: 1
top level: BBa_I5400
location: https://synbiohub.org/public/igem/BBa_I5400/annotation978408/range978408/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I5400/annotation978408/range978408/1
type: Range
persistent identity: range978408
display id: range978408
version: 1
direction: direction/forward
top level: BBa_I5400
start: 1927
end: 1938
orientation: inline

BBa_I3302
type: ComponentDefinition
persistent identity: BBa I3302
display id: BBa_I3302
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tar-EnvZ attractant receiver device (R1075.B0034.C0082.B0015.R0084)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-28T12:00:00Z
modified: 2015-08-31T04:07:41Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Constitutive promoter makes Tar-EnvZ fusion protein, which binds aspartate to create TIPS.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0010
component: BBa_R1075
component: BBa_R0084
component: BBa_B0012
component: BBa_B0034
component: BBa_C0082
sequence annotation: BBa_B0034
sequence annotation: BBa_C0082
sequence annotation: BBa_R0084
sequence annotation: BBa_B0012
sequence annotation: BBa_R1075
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_I3302_sequence/1

BBa_B0010
type: Component
persistent identity: component956056
display id: component956056
version: 1
top level: BBa_I3302
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R1075
type: Component
persistent identity: component956029
display id: component956029
version: 1
top level: BBa_I3302
access: public
definition: https://synbiohub.org/public/igem/BBa_R1075/1

BBa_R0084
type: Component
persistent identity: component956086
display id: component956086
version: 1
top level: BBa_I3302
access: public
definition: https://synbiohub.org/public/igem/BBa_R0084/1

BBa_B0012
type: Component
persistent identity: component956066
display id: component956066
version: 1
top level: BBa_I3302
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component956034
display id: component956034
version: 1
top level: BBa_I3302
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0082
type: Component
persistent identity: component956050
display id: component956050
version: 1
top level: BBa_I3302
access: public
definition: https://synbiohub.org/public/igem/BBa_C0082/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation956034
display id: annotation956034
version: 1
top level: BBa_I3302
location: https://synbiohub.org/public/igem/BBa_I3302/annotation956034/range956034/1
component: BBa_B0034

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type: Range
persistent identity: range956034
display id: range956034
version: 1
direction: direction/forward
top level: BBa_I3302
start: 58
end: 69
orientation: inline

BBa_C0082
type: SequenceAnnotation
persistent identity: annotation956050
display id: annotation956050
version: 1
top level: BBa_I3302
location: https://synbiohub.org/public/igem/BBa_I3302/annotation956050/range956050/1
component: BBa_C0082

https://synbiohub.org/public/igem/BBa_I3302/annotation956050/range956050/1
type: Range
persistent identity: range956050
display id: range956050
version: 1
direction: direction/forward
top level: BBa_I3302
start: 76
end: 1566
orientation: inline

BBa_R0084
type: SequenceAnnotation
persistent identity: annotation956086
display id: annotation956086
version: 1
top level: BBa_I3302
location: https://synbiohub.org/public/igem/BBa_I3302/annotation956086/range956086/1
component: BBa_R0084

https://synbiohub.org/public/igem/BBa_I3302/annotation956086/range956086/1
type: Range
persistent identity: range956086
display id: range956086
version: 1
direction: direction/forward
top level: BBa_I3302
start: 1737
end: 1844
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation956066
display id: annotation956066
version: 1
top level: BBa_I3302
location: https://synbiohub.org/public/igem/BBa_I3302/annotation956066/range956066/1
component: BBa_B0012

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persistent identity: range956066
display id: range956066
version: 1
direction: direction/forward
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start: 1688
end: 1728
orientation: inline

BBa_R1075
type: SequenceAnnotation
persistent identity: annotation956029
display id: annotation956029
version: 1
top level: BBa_I3302
location: https://synbiohub.org/public/igem/BBa_I3302/annotation956029/range956029/1
component: BBa_R1075

https://synbiohub.org/public/igem/BBa_I3302/annotation956029/range956029/1
type: Range
persistent identity: range956029
display id: range956029
version: 1
direction: direction/forward
top level: BBa_I3302
start: 1
end: 49
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation956056
display id: annotation956056
version: 1
top level: BBa_I3302
location: https://synbiohub.org/public/igem/BBa_I3302/annotation956056/range956056/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I3302/annotation956056/range956056/1
type: Range
persistent identity: range956056
display id: range956056
version: 1
direction: direction/forward
top level: BBa_I3302
start: 1600
end: 1679
orientation: inline

BBa_I13025
type: ComponentDefinition
persistent identity: BBa I13025
display id: BBa_I13025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mnt QPI Intermediate
creator: jenmitch
created: 2004-07-14T11:00:00Z
modified: 2015-08-31T04:07:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 159
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_C0072
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_C0072
sequence: https://synbiohub.org/public/igem/BBa_I13025_sequence/1

BBa_C0072
type: Component
persistent identity: component939424
display id: component939424
version: 1
top level: BBa_I13025
access: public
definition: https://synbiohub.org/public/igem/BBa_C0072/1

BBa_B0034
type: Component
persistent identity: component939411
display id: component939411
version: 1
top level: BBa_I13025
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation939411
display id: annotation939411
version: 1
top level: BBa_I13025
location: https://synbiohub.org/public/igem/BBa_I13025/annotation939411/range939411/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13025/annotation939411/range939411/1
type: Range
persistent identity: range939411
display id: range939411
version: 1
direction: direction/forward
top level: BBa_I13025
start: 1
end: 12
orientation: inline

BBa_C0072
type: SequenceAnnotation
persistent identity: annotation939424
display id: annotation939424
version: 1
top level: BBa_I13025
location: https://synbiohub.org/public/igem/BBa_I13025/annotation939424/range939424/1
component: BBa_C0072

https://synbiohub.org/public/igem/BBa_I13025/annotation939424/range939424/1
type: Range
persistent identity: range939424
display id: range939424
version: 1
direction: direction/forward
top level: BBa_I13025
start: 19
end: 306
orientation: inline

BBa_I13454
type: ComponentDefinition
persistent identity: BBa I13454
display id: BBa_I13454
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Insertion site #2
creator: jkm
created: 2005-06-19T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Contains a XmaI site and an AvrII site. A separate (non-BB) part contains a complete BB site within XmaI/AvrII sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: XmaI site
sequence annotation: AvrII site
sequence: https://synbiohub.org/public/igem/BBa_I13454_sequence/1

XmaI site
type: SequenceAnnotation
persistent identity: annotation1526494
display id: annotation1526494
version: 1
top level: BBa_I13454
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I13454/annotation1526494/range1526494/1

https://synbiohub.org/public/igem/BBa_I13454/annotation1526494/range1526494/1
type: Range
persistent identity: range1526494
display id: range1526494
version: 1
direction: direction/forward
top level: BBa_I13454
start: 1
end: 6
orientation: inline

AvrII site
type: SequenceAnnotation
persistent identity: annotation1526495
display id: annotation1526495
version: 1
top level: BBa_I13454
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I13454/annotation1526495/range1526495/1

https://synbiohub.org/public/igem/BBa_I13454/annotation1526495/range1526495/1
type: Range
persistent identity: range1526495
display id: range1526495
version: 1
direction: direction/forward
top level: BBa_I13454
start: 47
end: 52
orientation: inline

BBa_I9124
type: ComponentDefinition
persistent identity: BBa I9124
display id: BBa_I9124
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: test (rhlRI system) R0071.E0432.10500.E0422.I0500.I9027.I9024
creator: crackdots
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:08:11Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0012
component: BBa_B0034
component: BBa_C0071
component: BBa_R1075
component: BBa_E0032
component: BBa_E0022
component: BBa_B0010
component: BBa_R0071
component: BBa_C0070
component: BBa_I0500
sequence annotation: BBa_B0012
sequence annotation: BBa_C0071
sequence annotation: BBa_C0070
sequence annotation: BBa_I0500
sequence annotation: BBa_E0032
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_R0071
sequence annotation: BBa_R1075
sequence annotation: BBa_E0022
sequence: https://synbiohub.org/public/igem/BBa_I9124_sequence/1

BBa_B0012
type: Component
persistent identity: component991245
display id: component991245
version: 1
top level: BBa_I9124
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component991295
display id: component991295
version: 1
top level: BBa_I9124
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0071
type: Component
persistent identity: component991347
display id: component991347
version: 1
top level: BBa_I9124
access: public
definition: https://synbiohub.org/public/igem/BBa_C0071/1

BBa_R1075
type: Component
persistent identity: component991332
display id: component991332
version: 1
top level: BBa_I9124
access: public
definition: https://synbiohub.org/public/igem/BBa_R1075/1

BBa_B0012
type: Component
persistent identity: component991321
display id: component991321
version: 1
top level: BBa_I9124
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0032
type: Component
persistent identity: component991227
display id: component991227
version: 1
top level: BBa_I9124
access: public
definition: https://synbiohub.org/public/igem/BBa_E0032/1

BBa_E0022
type: Component
persistent identity: component991267
display id: component991267
version: 1
top level: BBa_I9124
access: public
definition: https://synbiohub.org/public/igem/BBa_E0022/1

BBa_B0010
type: Component
persistent identity: component991275
display id: component991275
version: 1
top level: BBa_I9124
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
type: Component
persistent identity: component991353
display id: component991353
version: 1
top level: BBa_I9124
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
type: Component
persistent identity: component991311
display id: component991311
version: 1
top level: BBa_I9124
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0071
type: Component
persistent identity: component991210
display id: component991210
version: 1
top level: BBa_I9124
access: public
definition: https://synbiohub.org/public/igem/BBa_R0071/1

BBa_B0012
type: Component
persistent identity: component991285
display id: component991285
version: 1
top level: BBa_I9124
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0070
type: Component
persistent identity: component991305
display id: component991305
version: 1
top level: BBa_I9124
access: public
definition: https://synbiohub.org/public/igem/BBa_C0070/1

BBa_B0034
type: Component
persistent identity: component991255
display id: component991255
version: 1
top level: BBa_I9124
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component991235
display id: component991235
version: 1
top level: BBa_I9124
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component991215
display id: component991215
version: 1
top level: BBa_I9124
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I0500
type: Component
persistent identity: component991290
display id: component991290
version: 1
top level: BBa_I9124
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0034
type: Component
persistent identity: component991337
display id: component991337
version: 1
top level: BBa_I9124
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component991363
display id: component991363
version: 1
top level: BBa_I9124
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_I0500
type: Component
persistent identity: component991250
display id: component991250
version: 1
top level: BBa_I9124
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation991363
display id: annotation991363
version: 1
top level: BBa_I9124
location: https://synbiohub.org/public/igem/BBa_I9124/annotation991363/range991363/1
component: BBa_B0012

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type: Range
persistent identity: range991363
display id: range991363
version: 1
direction: direction/forward
top level: BBa_I9124
start: 6136
end: 6176
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation991321
display id: annotation991321
version: 1
top level: BBa_I9124
location: https://synbiohub.org/public/igem/BBa_I9124/annotation991321/range991321/1
component: BBa_B0012

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type: Range
persistent identity: range991321
display id: range991321
version: 1
direction: direction/forward
top level: BBa_I9124
start: 5129
end: 5169
orientation: inline

BBa_C0071
type: SequenceAnnotation
persistent identity: annotation991347
display id: annotation991347
version: 1
top level: BBa_I9124
location: https://synbiohub.org/public/igem/BBa_I9124/annotation991347/range991347/1
component: BBa_C0071

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type: Range
persistent identity: range991347
display id: range991347
version: 1
direction: direction/forward
top level: BBa_I9124
start: 5253
end: 6014
orientation: inline

BBa_C0070
type: SequenceAnnotation
persistent identity: annotation991305
display id: annotation991305
version: 1
top level: BBa_I9124
location: https://synbiohub.org/public/igem/BBa_I9124/annotation991305/range991305/1
component: BBa_C0070

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type: Range
persistent identity: range991305
display id: range991305
version: 1
direction: direction/forward
top level: BBa_I9124
start: 4366
end: 5007
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation991290
display id: annotation991290
version: 1
top level: BBa_I9124
location: https://synbiohub.org/public/igem/BBa_I9124/annotation991290/range991290/1
component: BBa_I0500

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type: Range
persistent identity: range991290
display id: range991290
version: 1
direction: direction/forward
top level: BBa_I9124
start: 3130
end: 4339
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation991285
display id: annotation991285
version: 1
top level: BBa_I9124
location: https://synbiohub.org/public/igem/BBa_I9124/annotation991285/range991285/1
component: BBa_B0012

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type: Range
persistent identity: range991285
display id: range991285
version: 1
direction: direction/forward
top level: BBa_I9124
start: 3081
end: 3121
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation991227
display id: annotation991227
version: 1
top level: BBa_I9124
location: https://synbiohub.org/public/igem/BBa_I9124/annotation991227/range991227/1
component: BBa_E0032

https://synbiohub.org/public/igem/BBa_I9124/annotation991227/range991227/1
type: Range
persistent identity: range991227
display id: range991227
version: 1
direction: direction/forward
top level: BBa_I9124
start: 80
end: 841
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation991275
display id: annotation991275
version: 1
top level: BBa_I9124
location: https://synbiohub.org/public/igem/BBa_I9124/annotation991275/range991275/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I9124/annotation991275/range991275/1
type: Range
persistent identity: range991275
display id: range991275
version: 1
direction: direction/forward
top level: BBa_I9124
start: 2993
end: 3072
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation991295
display id: annotation991295
version: 1
top level: BBa_I9124
location: https://synbiohub.org/public/igem/BBa_I9124/annotation991295/range991295/1
component: BBa_B0034

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type: Range
persistent identity: range991295
display id: range991295
version: 1
direction: direction/forward
top level: BBa_I9124
start: 4348
end: 4359
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation991337
display id: annotation991337
version: 1
top level: BBa_I9124
location: https://synbiohub.org/public/igem/BBa_I9124/annotation991337/range991337/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I9124/annotation991337/range991337/1
type: Range
persistent identity: range991337
display id: range991337
version: 1
direction: direction/forward
top level: BBa_I9124
start: 5235
end: 5246
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation991250
display id: annotation991250
version: 1
top level: BBa_I9124
location: https://synbiohub.org/public/igem/BBa_I9124/annotation991250/range991250/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_I9124/annotation991250/range991250/1
type: Range
persistent identity: range991250
display id: range991250
version: 1
direction: direction/forward
top level: BBa_I9124
start: 987
end: 2196
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation991235
display id: annotation991235
version: 1
top level: BBa_I9124
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description: Lambda cI/LacI ts (mut 265) RS flip-flop
creator: ytwang
created: 2005-06-27T11:00:00Z
modified: 2015-08-31T04:08:19Z
discontinued: true
dominant: false
experience: experience/None
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status: status/Deleted
bookmark: false
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description: Booperator test: RhlR biasing, RhlR/LuxR crosstalk
creator: Srini Devadas, David Gray, Ronny Krashinsky, Debra Lin, and Chris Zheng Liu
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:08:10Z
discontinued: false
dominant: false
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sample status: Not in stock
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BBa_B0012
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BBa_B0012
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BBa_R0040
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BBa_B0012
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BBa_B0010
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BBa_C0062
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BBa_B0034
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BBa_B0010
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BBa_B0012
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BBa_C0071
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BBa_B0034
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BBa_B0034
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BBa_B0010
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BBa_B0034
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BBa_R0050
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BBa_B0010
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BBa_R0071
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BBa_B0010
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BBa_R0040
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BBa_I3510
type: ComponentDefinition
persistent identity: BBa I3510
display id: BBa_I3510
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Quorum receiver device (R0063.I0462.R0062)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-28T12:00:00Z
modified: 2015-08-31T04:07:41Z
discontinued: false
dominant: true
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Quorum receiver has luxR device under the control of lux pL promoter to produce luxR, which binds HSL and activates lux pR promoter.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0010
component: BBa_B0012
component: BBa_R0062
component: BBa_C0062
component: BBa_R0063
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sequence annotation: BBa_C0062
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BBa_B0010
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BBa_C0062
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description: Booperator: LuxR/RhlR/aiia, monocistronic
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Booperator aiia test: degradation of HSL "A" by aiia
creator: Srini Devadas, David Gray, Ronny Krashinsky, Debra Lin, and Chris Zheng Liu
created: 2004-01-31T12:00:00Z
modified: 2015-08-31T04:08:11Z
discontinued: false
dominant: false
group u list: _1_
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sample status: Not in stock
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BBa_B0010
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group u list: _1_
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persistent identity: BBa I3600
display id: BBa_I3600
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Test: Chemotaxis response to secreted aspartate, sender cells (I3100.I3200.E0422)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-28T12:00:00Z
modified: 2015-08-31T04:07:41Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Tests the chemotaxis cell-cell signalling machinery based on aspartate secretions. Sender cells are nonmotile and have a trigger controlling the AspA attractant sender and CFP reporter indicating transcription of aspA. Receiver cells have a constitutive promoter controlling YFP reporter. Chemotaxis can be observed by the movement of the YFP-marked receiver cells.
mutable notes: Sender cells are the nonchemotactic E. coli strain RP5232. Receiver cells are wildtype E. coli.
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star: false
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type: ComponentDefinition
persistent identity: BBa I2023
display id: BBa_I2023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Full Heat Trigger Test (R1075.Q04811.E0432)
creator: Alvin Carter Powers, Yong-Su Jin (Fighting Darwins)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:39Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: heat trigger test: tests the trigger as an entire device; constitutive promoter should prevent TIPs leakage (indicated by the reporter), and when heat shocked, reporter should produce YFP
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
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BBa_R0040
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BBa_C0050
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BBa_R0040
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BBa_B0010
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BBa_R0062
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BBa_R0062
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BBa_B0034
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BBa_B0010
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BBa_C0071
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BBa_B0010
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BBa_B0012
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BBa_B0010
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BBa_E0022
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BBa_B0034
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BBa_B0012
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BBa_C0062
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BBa_B0012
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BBa_B0034
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BBa_C0040
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BBa_E0032
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BBa_B0010
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BBa_B0012
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BBa_R0040
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BBa_B0034
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BBa_B0012
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BBa_R0050
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BBa_B0034
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BBa_B0012
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BBa_B0012
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BBa_B0010
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BBa_B0010
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BBa_B0034
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BBa_B0010
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BBa_B0034
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BBa_R0062
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BBa_B0034
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BBa_R0050
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BBa_R0062
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BBa_R0040
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BBa_B0010
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BBa_C0062
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BBa_B0034
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BBa_B0012
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BBa_B0012
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BBa_B0012
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BBa_C0050
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BBa_B0010
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BBa_E0022
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BBa_B0010
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BBa_C0071
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BBa_B0012
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BBa_R0040
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BBa_I9108
type: ComponentDefinition
persistent identity: BBa I9108
display id: BBa_I9108
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: test (tp901-cI nor) I9107.I9106.I9106
creator: crackdots
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:08:11Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_I0500
component: BBa_B0034
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component: BBa_B0012
component: BBa_C0075
component: BBa_E0022
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Test: Switch aspartate excretion (I3410.I3200.I3411.E0432.I3100.I3400.I3101.I3400.I3102.I3401)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-28T12:00:00Z
modified: 2015-08-31T04:07:41Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Tests ability of switch to output aspartate given particular inputs. The remaining testbed is analogous to the switch functionality testbed (I3420).
mutable notes: The layout of the switch mimics the layout of the proposed Polkadorks E. colibrator (I3000). Two switch outputs are insulated by appearing first. Hk022cI is attached to the AspA attractant sender (I3410.I3200). Lambda cI is attached to the YFP reporter (I3411.E0432). The three individually controlled trigger inputs follow the outputs in the layout. Lac trigger controls one hk022cI input (I3100.I3400), and TetR trigger controls the other (I3101.I3400). The dual control forms the built-in OR gate in one of the switch inputs. Finally, AraC trigger controls the lambda cI input (I3102.I3401).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
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description: TetR trigger device (R1075.B0034.C0040.B0015.R0040)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-28T12:00:00Z
modified: 2015-08-31T04:07:41Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
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sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Tetracycline-induced trigger using tetR.
owned by: james
owned by: myers
star: false
type: DnaRegion
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component: BBa_C0040
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description: LacI repressed lambda cI(LVA) + LuxI(LVA) + LacZalpha
creator: Jeff Tabor
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discontinued: false
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experience: experience/Issues
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bookmark: false
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description: Synchronator design 3, Output-
creator: SMUG: Oscillator
created: 2004-08-15T11:00:00Z
modified: 2015-08-31T04:07:41Z
discontinued: false
dominant: false
experience: experience/None
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status: status/Unavailable
bookmark: false
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display id: component1025925
version: 1
top level: BBa_I13900
access: public
definition: https://synbiohub.org/public/igem/BBa_C0040/1

BBa_B0012
type: Component
persistent identity: component1025991
display id: component1025991
version: 1
top level: BBa_I13900
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0061
type: Component
persistent identity: component1025974
display id: component1025974
version: 1
top level: BBa_I13900
access: public
definition: https://synbiohub.org/public/igem/BBa_C0061/1

BBa_B0010
type: Component
persistent identity: component1025981
display id: component1025981
version: 1
top level: BBa_I13900
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1025915
display id: component1025915
version: 1
top level: BBa_I13900
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component1025964
display id: component1025964
version: 1
top level: BBa_I13900
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I13201
type: Component
persistent identity: component1025910
display id: component1025910
version: 1
top level: BBa_I13900
access: public
definition: https://synbiohub.org/public/igem/BBa_I13201/1

BBa_B0012
type: Component
persistent identity: component1025941
display id: component1025941
version: 1
top level: BBa_I13900
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0040
type: Component
persistent identity: component1025956
display id: component1025956
version: 1
top level: BBa_I13900
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0010
type: Component
persistent identity: component1025931
display id: component1025931
version: 1
top level: BBa_I13900
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1025941
display id: annotation1025941
version: 1
top level: BBa_I13900
location: https://synbiohub.org/public/igem/BBa_I13900/annotation1025941/range1025941/1
component: BBa_B0012

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type: Range
persistent identity: range1025941
display id: range1025941
version: 1
direction: direction/forward
top level: BBa_I13900
start: 1831
end: 1871
orientation: inline

BBa_I13201
type: SequenceAnnotation
persistent identity: annotation1025910
display id: annotation1025910
version: 1
top level: BBa_I13900
location: https://synbiohub.org/public/igem/BBa_I13900/annotation1025910/range1025910/1
component: BBa_I13201

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type: Range
persistent identity: range1025910
display id: range1025910
version: 1
direction: direction/forward
top level: BBa_I13900
start: 1
end: 1023
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1025964
display id: annotation1025964
version: 1
top level: BBa_I13900
location: https://synbiohub.org/public/igem/BBa_I13900/annotation1025964/range1025964/1
component: BBa_B0034

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type: Range
persistent identity: range1025964
display id: range1025964
version: 1
direction: direction/forward
top level: BBa_I13900
start: 1942
end: 1953
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation1025925
display id: annotation1025925
version: 1
top level: BBa_I13900
location: https://synbiohub.org/public/igem/BBa_I13900/annotation1025925/range1025925/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_I13900/annotation1025925/range1025925/1
type: Range
persistent identity: range1025925
display id: range1025925
version: 1
direction: direction/forward
top level: BBa_I13900
start: 1050
end: 1709
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1025981
display id: annotation1025981
version: 1
top level: BBa_I13900
location: https://synbiohub.org/public/igem/BBa_I13900/annotation1025981/range1025981/1
component: BBa_B0010

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type: Range
persistent identity: range1025981
display id: range1025981
version: 1
direction: direction/forward
top level: BBa_I13900
start: 2611
end: 2690
orientation: inline

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation1025974
display id: annotation1025974
version: 1
top level: BBa_I13900
location: https://synbiohub.org/public/igem/BBa_I13900/annotation1025974/range1025974/1
component: BBa_C0061

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type: Range
persistent identity: range1025974
display id: range1025974
version: 1
direction: direction/forward
top level: BBa_I13900
start: 1960
end: 2577
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1025931
display id: annotation1025931
version: 1
top level: BBa_I13900
location: https://synbiohub.org/public/igem/BBa_I13900/annotation1025931/range1025931/1
component: BBa_B0010

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type: Range
persistent identity: range1025931
display id: range1025931
version: 1
direction: direction/forward
top level: BBa_I13900
start: 1743
end: 1822
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1025991
display id: annotation1025991
version: 1
top level: BBa_I13900
location: https://synbiohub.org/public/igem/BBa_I13900/annotation1025991/range1025991/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13900/annotation1025991/range1025991/1
type: Range
persistent identity: range1025991
display id: range1025991
version: 1
direction: direction/forward
top level: BBa_I13900
start: 2699
end: 2739
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1025956
display id: annotation1025956
version: 1
top level: BBa_I13900
location: https://synbiohub.org/public/igem/BBa_I13900/annotation1025956/range1025956/1
component: BBa_R0040

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type: Range
persistent identity: range1025956
display id: range1025956
version: 1
direction: direction/forward
top level: BBa_I13900
start: 1880
end: 1933
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1025915
display id: annotation1025915
version: 1
top level: BBa_I13900
location: https://synbiohub.org/public/igem/BBa_I13900/annotation1025915/range1025915/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13900/annotation1025915/range1025915/1
type: Range
persistent identity: range1025915
display id: range1025915
version: 1
direction: direction/forward
top level: BBa_I13900
start: 1032
end: 1043
orientation: inline

BBa_I13930
type: ComponentDefinition
persistent identity: BBa I13930
display id: BBa_I13930
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: c1 Delay Oscillator Intermediate Construct (I13202.C0051.B0015)
creator: robertb
created: 2004-08-03T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 159
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Natural Lux Cassette + c1 + Term. This construct will be put together with the synthetic c1 promoter being synthesized by Caltech and the various AiiA Generation Constructs to create a variety of c1 Delay Oscillators.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_C0051
component: BBa_I13202
component: BBa_B0015
sequence annotation: BBa_C0051
sequence annotation: BBa_B0015
sequence annotation: BBa_I13202
sequence: https://synbiohub.org/public/igem/BBa_I13930_sequence/1

BBa_C0051
type: Component
persistent identity: component2226080
display id: component2226080
version: 1
top level: BBa_I13930
access: public
definition: https://synbiohub.org/public/igem/BBa_C0051/1

BBa_I13202
type: Component
persistent identity: component2226076
display id: component2226076
version: 1
top level: BBa_I13930
access: public
definition: https://synbiohub.org/public/igem/BBa_I13202/1

BBa_B0015
type: Component
persistent identity: component2226087
display id: component2226087
version: 1
top level: BBa_I13930
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation2226080
display id: annotation2226080
version: 1
top level: BBa_I13930
location: https://synbiohub.org/public/igem/BBa_I13930/annotation2226080/range2226080/1
component: BBa_C0051

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type: Range
persistent identity: range2226080
display id: range2226080
version: 1
direction: direction/reverse
top level: BBa_I13930
start: 810
end: 1584
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2226087
display id: annotation2226087
version: 1
top level: BBa_I13930
location: https://synbiohub.org/public/igem/BBa_I13930/annotation2226087/range2226087/1
component: BBa_B0015

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type: Range
persistent identity: range2226087
display id: range2226087
version: 1
direction: direction/reverse
top level: BBa_I13930
start: 1593
end: 1721
orientation: inline

BBa_I13202
type: SequenceAnnotation
persistent identity: annotation2226076
display id: annotation2226076
version: 1
top level: BBa_I13930
location: https://synbiohub.org/public/igem/BBa_I13930/annotation2226076/range2226076/1
component: BBa_I13202

https://synbiohub.org/public/igem/BBa_I13930/annotation2226076/range2226076/1
type: Range
persistent identity: range2226076
display id: range2226076
version: 1
direction: direction/reverse
top level: BBa_I13930
start: 1
end: 803
orientation: inline

BBa_I12039
type: ComponentDefinition
persistent identity: BBa I12039
display id: BBa_I12039
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Test construct for BBa_I12036
creator: ryhsiao
created: 2004-08-18T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 148
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Expression of lambda cI controlled by IPTG (requires LacIq strain). Expression of 434 cI controlled by arabinose. If BBa_I12036 promoter works, YFP is activated by lambda cI and repressed by 434 cI.
mutable notes: Designed in the exact same way as BBa_I12028 (Test construct for I12006) except with BBa_I12036 swapped in for BBa_I12006
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_I12024
component: BBa_I12027
component: BBa_I12036
component: BBa_E0430
sequence annotation: BBa_I12027
sequence annotation: BBa_I12036
sequence annotation: BBa_I12024
sequence annotation: BBa_E0430
sequence: https://synbiohub.org/public/igem/BBa_I12039_sequence/1

BBa_I12024
type: Component
persistent identity: component2234976
display id: component2234976
version: 1
top level: BBa_I12039
access: public
definition: https://synbiohub.org/public/igem/BBa_I12024/1

BBa_I12027
type: Component
persistent identity: component2234990
display id: component2234990
version: 1
top level: BBa_I12039
access: public
definition: https://synbiohub.org/public/igem/BBa_I12027/1

BBa_I12036
type: Component
persistent identity: component2234998
display id: component2234998
version: 1
top level: BBa_I12039
access: public
definition: https://synbiohub.org/public/igem/BBa_I12036/1

BBa_E0430
type: Component
persistent identity: component2235008
display id: component2235008
version: 1
top level: BBa_I12039
access: public
definition: https://synbiohub.org/public/igem/BBa_E0430/1

BBa_I12027
type: SequenceAnnotation
persistent identity: annotation2234990
display id: annotation2234990
version: 1
top level: BBa_I12039
location: https://synbiohub.org/public/igem/BBa_I12039/annotation2234990/range2234990/1
component: BBa_I12027

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type: Range
persistent identity: range2234990
display id: range2234990
version: 1
direction: direction/reverse
top level: BBa_I12039
start: 1002
end: 3068
orientation: inline

BBa_I12036
type: SequenceAnnotation
persistent identity: annotation2234998
display id: annotation2234998
version: 1
top level: BBa_I12039
location: https://synbiohub.org/public/igem/BBa_I12039/annotation2234998/range2234998/1
component: BBa_I12036

https://synbiohub.org/public/igem/BBa_I12039/annotation2234998/range2234998/1
type: Range
persistent identity: range2234998
display id: range2234998
version: 1
direction: direction/reverse
top level: BBa_I12039
start: 3077
end: 3167
orientation: inline

BBa_I12024
type: SequenceAnnotation
persistent identity: annotation2234976
display id: annotation2234976
version: 1
top level: BBa_I12039
location: https://synbiohub.org/public/igem/BBa_I12039/annotation2234976/range2234976/1
component: BBa_I12024

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type: Range
persistent identity: range2234976
display id: range2234976
version: 1
direction: direction/reverse
top level: BBa_I12039
start: 1
end: 993
orientation: inline

BBa_E0430
type: SequenceAnnotation
persistent identity: annotation2235008
display id: annotation2235008
version: 1
top level: BBa_I12039
location: https://synbiohub.org/public/igem/BBa_I12039/annotation2235008/range2235008/1
component: BBa_E0430

https://synbiohub.org/public/igem/BBa_I12039/annotation2235008/range2235008/1
type: Range
persistent identity: range2235008
display id: range2235008
version: 1
direction: direction/reverse
top level: BBa_I12039
start: 3176
end: 4053
orientation: inline

BBa_I13941
type: ComponentDefinition
persistent identity: BBa I13941
display id: BBa_I13941
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Exogenous LuxI Relaxation Oscillator Test Construct Intermediate (R0062.C0260.E0424)
creator: robertb
created: 2004-08-22T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 159
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Intermediate construct for Chris' relaxation oscillator test constructs.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0010
component: BBa_B0034
component: BBa_R0062
component: BBa_C0060
component: BBa_B0012
component: BBa_E0024
sequence annotation: BBa_R0062
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_E0024
sequence annotation: BBa_C0060
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I13941_sequence/1

BBa_B0010
type: Component
persistent identity: component1060515
display id: component1060515
version: 1
top level: BBa_I13941
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1060508
display id: component1060508
version: 1
top level: BBa_I13941
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component1060485
display id: component1060485
version: 1
top level: BBa_I13941
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0062
type: Component
persistent identity: component1060477
display id: component1060477
version: 1
top level: BBa_I13941
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_C0060
type: Component
persistent identity: component1060500
display id: component1060500
version: 1
top level: BBa_I13941
access: public
definition: https://synbiohub.org/public/igem/BBa_C0060/1

BBa_B0012
type: Component
persistent identity: component1060525
display id: component1060525
version: 1
top level: BBa_I13941
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0024
type: Component
persistent identity: component1060510
display id: component1060510
version: 1
top level: BBa_I13941
access: public
definition: https://synbiohub.org/public/igem/BBa_E0024/1

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation1060477
display id: annotation1060477
version: 1
top level: BBa_I13941
location: https://synbiohub.org/public/igem/BBa_I13941/annotation1060477/range1060477/1
component: BBa_R0062

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type: Range
persistent identity: range1060477
display id: range1060477
version: 1
direction: direction/forward
top level: BBa_I13941
start: 1
end: 55
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1060508
display id: annotation1060508
version: 1
top level: BBa_I13941
location: https://synbiohub.org/public/igem/BBa_I13941/annotation1060508/range1060508/1
component: BBa_B0034

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type: Range
persistent identity: range1060508
display id: range1060508
version: 1
direction: direction/forward
top level: BBa_I13941
start: 904
end: 915
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1060515
display id: annotation1060515
version: 1
top level: BBa_I13941
location: https://synbiohub.org/public/igem/BBa_I13941/annotation1060515/range1060515/1
component: BBa_B0010

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type: Range
persistent identity: range1060515
display id: range1060515
version: 1
direction: direction/forward
top level: BBa_I13941
start: 1692
end: 1771
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1060485
display id: annotation1060485
version: 1
top level: BBa_I13941
location: https://synbiohub.org/public/igem/BBa_I13941/annotation1060485/range1060485/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13941/annotation1060485/range1060485/1
type: Range
persistent identity: range1060485
display id: range1060485
version: 1
direction: direction/forward
top level: BBa_I13941
start: 64
end: 75
orientation: inline

BBa_E0024
type: SequenceAnnotation
persistent identity: annotation1060510
display id: annotation1060510
version: 1
top level: BBa_I13941
location: https://synbiohub.org/public/igem/BBa_I13941/annotation1060510/range1060510/1
component: BBa_E0024

https://synbiohub.org/public/igem/BBa_I13941/annotation1060510/range1060510/1
type: Range
persistent identity: range1060510
display id: range1060510
version: 1
direction: direction/forward
top level: BBa_I13941
start: 922
end: 1683
orientation: inline

BBa_C0060
type: SequenceAnnotation
persistent identity: annotation1060500
display id: annotation1060500
version: 1
top level: BBa_I13941
location: https://synbiohub.org/public/igem/BBa_I13941/annotation1060500/range1060500/1
component: BBa_C0060

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type: Range
persistent identity: range1060500
display id: range1060500
version: 1
direction: direction/forward
top level: BBa_I13941
start: 82
end: 870
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1060525
display id: annotation1060525
version: 1
top level: BBa_I13941
location: https://synbiohub.org/public/igem/BBa_I13941/annotation1060525/range1060525/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13941/annotation1060525/range1060525/1
type: Range
persistent identity: range1060525
display id: range1060525
version: 1
direction: direction/forward
top level: BBa_I13941
start: 1780
end: 1820
orientation: inline

BBa_I2024
type: ComponentDefinition
persistent identity: BBa I2024
display id: BBa_I2024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LasI Sender Test (I0500.B0034.C0078.B0015)
creator: Sara Neves, Chris Walsh (Fighting Darwins)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:39Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: lasI test: with input of TIPs from I0500, determines how much chemical signal is ultimately made by the lasI protein amount of signal can be determined chemically (?)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_I0500
component: BBa_B0010
component: BBa_B0034
component: BBa_C0078
component: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_I0500
sequence annotation: BBa_C0078
sequence: https://synbiohub.org/public/igem/BBa_I2024_sequence/1

BBa_I0500
type: Component
persistent identity: component947523
display id: component947523
version: 1
top level: BBa_I2024
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0010
type: Component
persistent identity: component947544
display id: component947544
version: 1
top level: BBa_I2024
access: public
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display id: BBa_J06963
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 434 cI/lambda cI RS flip-flop with EYFP
creator: Yves Wang
created: 2005-07-31T11:00:00Z
modified: 2015-08-31T04:08:19Z
discontinued: false
dominant: false
group u list: _20_
m user id: 0
owner id: 340
owning group id: 20
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: RS flip-flop with lambda cI as the set and 434 cI as the reset.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_R0052
component: BBa_B0012
component: BBa_R0051
component: BBa_B0034
component: BBa_B0010
component: BBa_E0030
component: BBa_C0051
component: BBa_C0052
sequence annotation: BBa_B0034
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sequence annotation: BBa_R0051
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sequence annotation: BBa_B0012
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BBa_B0034
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BBa_I13910
type: ComponentDefinition
persistent identity: BBa I13910
display id: BBa_I13910
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AiiA (+LVA) Tri-part (B0034.C0060.B0015)
creator: robertb
created: 2004-08-01T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 159
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Elowitz RBS + Aiia (+LVA) + Term
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0034
component: BBa_B0012
component: BBa_C0060
component: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_C0060
sequence: https://synbiohub.org/public/igem/BBa_I13910_sequence/1

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access: public
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version: 1
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BBa_B0010
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version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

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type: ComponentDefinition
persistent identity: BBa I13976
display id: BBa_I13976
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: intermediate for lac relaxation oscillator
creator: jasonk
created: 2004-10-04T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 2
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Intermediate for lac relaxation oscillator (final stage)
mutable notes: There is a single base insertion (of an A) at position 286, this is in the mixed site between R0011 and B0034 (first subpart of E0034) so suspect it should have little effect on device performance. But no experimental confirmation.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_E0434
component: BBa_R0011
sequence annotation: BBa_R0011
sequence annotation: BBa_E0434
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display id: component2221652
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type: ComponentDefinition
persistent identity: BBa I1101
display id: BBa_I1101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: -- No description --
creator: 2
created: 2004-01-28T12:00:00Z
modified: 2015-08-31T04:07:30Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_C0100
component: BBa_B0010
component: BBa_B0012
component: BBa_C0080
component: BBa_E0032
component: BBa_R1051
component: BBa_I0500
sequence annotation: BBa_R1051
sequence annotation: BBa_B0012
sequence annotation: BBa_C0100
sequence annotation: BBa_E0032
sequence annotation: BBa_B0010
sequence annotation: BBa_I0500
sequence annotation: BBa_C0080
sequence: https://synbiohub.org/public/igem/BBa_I1101_sequence/1

BBa_C0100
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BBa_B0010
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BBa_B0012
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definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
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BBa_C0100
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BBa_B0010
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BBa_C0080
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BBa_E0032
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definition: https://synbiohub.org/public/igem/BBa_E0032/1

BBa_R1051
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definition: https://synbiohub.org/public/igem/BBa_R1051/1

BBa_I0500
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access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_R1051
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component: BBa_R1051

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BBa_B0012
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BBa_C0100
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BBa_E0032
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BBa_C0100
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BBa_B0010
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BBa_I0500
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BBa_C0080
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BBa_B0012
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BBa_B0010
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display id: range965096
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start: 4461
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BBa_I13905
type: ComponentDefinition
persistent identity: BBa I13905
display id: BBa_I13905
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ring Oscillator Synchronization Device (lac)
creator: jasonk
created: 2004-09-22T11:00:00Z
modified: 2015-08-31T04:07:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 2
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This device is meant to be coupled to the elowitz repressilator plasmid to synchronization oscillations accross a population of cells. Sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0012
component: BBa_B0034
component: BBa_C0061
component: BBa_C0062
component: BBa_R0063
component: BBa_E0034
component: BBa_B0010
component: BBa_R0062
component: BBa_C0012
component: BBa_R0051
component: BBa_R0011
sequence annotation: BBa_B0034
sequence annotation: BBa_R0062
sequence annotation: BBa_E0034
sequence annotation: BBa_B0010
sequence annotation: BBa_C0012
sequence annotation: BBa_B0012
sequence annotation: BBa_C0061
sequence annotation: BBa_R0051
sequence annotation: BBa_C0062
sequence annotation: BBa_R0011
sequence annotation: BBa_R0063
sequence: https://synbiohub.org/public/igem/BBa_I13905_sequence/1

BBa_B0012
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BBa_B0034
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definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
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definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0061
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BBa_C0062
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BBa_R0063
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BBa_E0034
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BBa_B0010
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definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
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definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0062
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BBa_B0034
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BBa_C0012
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definition: https://synbiohub.org/public/igem/BBa_C0012/1

BBa_B0012
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definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
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definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
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BBa_R0051
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BBa_B0034
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definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0011
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BBa_B0034
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BBa_B0010
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BBa_B0034
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BBa_C0012
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description: Lac stochastic trigger device (R1075.B0034.C0012.B0015.R0010)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
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modified: 2015-08-31T04:07:41Z
discontinued: false
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group u list: _1_
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star: false
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description: test (penI QPI) I0500.E0422.I0500.I9002.E0432
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modified: 2015-08-31T04:08:11Z
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group u list: _1_
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 434 cI/lambda cI857 (E07700) RS flip-flop with EYFP
creator: Yves Wang
created: 2005-07-31T11:00:00Z
modified: 2015-08-31T04:08:19Z
discontinued: true
dominant: false
group u list: _20_
m user id: 0
owner id: 340
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sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: RS flip-flop with lambda cI (E07700) as the set and 434 cI as the reset. It will also take UV exposure as the set and heat (40??C) as the reset.
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type: ComponentDefinition
persistent identity: BBa I3002
display id: BBa_I3002
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: E. colibrator design 3, Polkadorks (I3410.I3200.E0422.I3510.I3401.I3411.I0460.I3100.I3400.I3300.I340
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:07:41Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: E. colibrator design 3 has the OR gate embedded within the switch via a double instance of I3400 Hk022cI switch input device. Design 3 has an aiiA mechanism turn on during the switch OFF state, as illustrated by I0460 AiiA device immediately following I3411 Lambda cI switch output device. This allows the OFF state to speed up reset of the system by degrading residual HSL once quorum threshold is reached.
mutable notes: The layout of E. colibrator (I3002) is designed to insulate the most sensitive devices, namely those in the switch that store state. The hk022cI switch output (ON) is insulated by appearing first. This output is then attached to the AspA attractant sender and the CFP reporter (I3410.I3200.E0422). The quorum receiver and its control of the lambda cI switch input (OFF) occur next (I3510.I3401) because leaky TIPS through this module will encourage the OFF state dicated by the downstream lambda cI switch output (I3411.I0460). Note that the OFF switch output leads to aiiA synthesis to promote HSL degradation and system reset in this design. The rest of the switch inputs occur downstream because they do not store state and therefore do not need to be as insulated from leaky TIPS. Lac trigger controls one hk022cI input (I3100.I3400), and Tar-EnvZ receptor (I3300 upstream ompC version) controls the other (I3300.I3400). The dual control forms the built-in OR gate in one of the switch inputs. Finally, the quorum sender device (I3500) is last because it is meant to be constitutively on at some basal level, so insulation is not required. Note: Depending on the efficacy of the OmpR promoter, this design can be implemented with any of I3300, I3301, I3302 (variations of Tar-EnvZ devices with different OmpR promoters). The default implementation uses I3300.
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BBa_B0034
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BBa_B0034
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BBa_C0012
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BBa_B0012
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BBa_R0062
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BBa_R0051
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BBa_B0012
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BBa_R0062
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BBa_C0083
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BBa_C0051
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display id: range1010144
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end: 5280
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BBa_B0012
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BBa_C0062
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end: 4279
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BBa_C0061
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display id: annotation1010476
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BBa_R1075
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BBa_B0034
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end: 6326
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BBa_C0050
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BBa_B0034
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BBa_B0010
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BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1010101
display id: annotation1010101
version: 1
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persistent identity: range1010101
display id: range1010101
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BBa_I15000
type: ComponentDefinition
persistent identity: BBa I15000
display id: BBa_I15000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lux activated cI repressed LacZalpha
creator: jtabor
created: 2004-05-21T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: true
dominant: false
experience: experience/None
group u list: _5_
m user id: 0
owner id: 88
owning group id: 7
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: System flanked on both sides by dual transcription terminators. Bears a joint Lux promoter-lambda operator, strong RBS, with LacZ alpha peptide.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0012
component: BBa_B0010
component: BBa_R0065
component: BBa_B0030
component: BBa_E0033
sequence annotation: BBa_E0033
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_B0030
sequence annotation: BBa_R0065
sequence: https://synbiohub.org/public/igem/BBa_I15000_sequence/1

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display id: component1754669
version: 1
top level: BBa_I15000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1754659
display id: component1754659
version: 1
top level: BBa_I15000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0065
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display id: component1754689
version: 1
top level: BBa_I15000
access: public
definition: https://synbiohub.org/public/igem/BBa_R0065/1

BBa_B0030
type: Component
persistent identity: component1754704
display id: component1754704
version: 1
top level: BBa_I15000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_E0033
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persistent identity: component1754727
display id: component1754727
version: 1
top level: BBa_I15000
access: public
definition: https://synbiohub.org/public/igem/BBa_E0033/1

BBa_B0012
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persistent identity: component1754743
display id: component1754743
version: 1
top level: BBa_I15000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
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persistent identity: component1754733
display id: component1754733
version: 1
top level: BBa_I15000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0033
type: SequenceAnnotation
persistent identity: annotation1754727
display id: annotation1754727
version: 1
top level: BBa_I15000
location: https://synbiohub.org/public/igem/BBa_I15000/annotation1754727/range1754727/1
component: BBa_E0033

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type: Range
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display id: range1754727
version: 1
direction: direction/forward
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start: 264
end: 616
orientation: inline

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display id: annotation1754669
version: 1
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end: 129
orientation: inline

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version: 1
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display id: range1754659
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direction: direction/forward
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orientation: inline

BBa_B0010
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version: 1
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display id: range1754733
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direction: direction/forward
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end: 729
orientation: inline

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display id: range1754743
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direction: direction/forward
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end: 778
orientation: inline

BBa_B0030
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version: 1
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persistent identity: range1754704
display id: range1754704
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start: 243
end: 257
orientation: inline

BBa_R0065
type: SequenceAnnotation
persistent identity: annotation1754689
display id: annotation1754689
version: 1
top level: BBa_I15000
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persistent identity: range1754689
display id: range1754689
version: 1
direction: direction/forward
top level: BBa_I15000
start: 138
end: 234
orientation: inline

BBa_I15002
type: ComponentDefinition
persistent identity: BBa I15002
display id: BBa_I15002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacI regulated cI/LuxI/LacZalpha
creator: jtabor
created: 2004-05-22T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: true
dominant: false
experience: experience/None
group u list: _5_
m user id: 0
owner id: 88
owning group id: 7
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: Tester module for BBa_I15001. Flanked by dual terminators. LacI regulated polycistronic cI/LuxI/LacZalpha
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0010
component: BBa_B0034
component: BBa_B0012
component: BBa_C0061
component: BBa_I1020
component: BBa_E0033
sequence annotation: BBa_B0012
sequence annotation: BBa_C0061
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_I1020
sequence annotation: BBa_E0033
sequence: https://synbiohub.org/public/igem/BBa_I15002_sequence/1

BBa_B0010
type: Component
persistent identity: component962781
display id: component962781
version: 1
top level: BBa_I15002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component962847
display id: component962847
version: 1
top level: BBa_I15002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component962902
display id: component962902
version: 1
top level: BBa_I15002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0061
type: Component
persistent identity: component962857
display id: component962857
version: 1
top level: BBa_I15002
access: public
definition: https://synbiohub.org/public/igem/BBa_C0061/1

BBa_B0010
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persistent identity: component962892
display id: component962892
version: 1
top level: BBa_I15002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component962791
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BBa_I1070
type: ComponentDefinition
persistent identity: BBa I1070
display id: BBa_I1070
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: XOR test/debug module
creator: Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:30Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part combines multiple Biobrick elements into a single module for testing and debugging elements of the XOR genetic gate. It tests systems that use cI protein as output. The promoter within the testing module is cI repressible. The advantage of constructing the test module de novo is the ease of transferring the entire unit to elements we would like to test within the XOR gate. YFP expression levels are the output of the test module and serve as a marker for system function.
mutable notes: synthetic element, using a combination of Biobrick parts--cI promoter, RBS, YFP, transcriptional terminators. used for testing systems that use cI protein as outputs.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: 2
sequence annotation: BBa_I1070
sequence annotation: YFP
sequence annotation: start
sequence annotation: SsrA
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2
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YFP
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start
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role: feature/tag
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type: ComponentDefinition
persistent identity: BBa I4002
display id: BBa_I4002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Prakash: Design 2
creator: Randy Rettberg
created: 2003-12-03T12:00:00Z
modified: 2015-08-31T04:07:41Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0032
component: BBa_B0010
component: BBa_B0030
component: BBa_R0010
component: BBa_R0063
component: BBa_R0040
component: BBa_E0032
component: BBa_B0012
component: BBa_C0062
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component: BBa_C0040
component: BBa_E0022
component: BBa_C0060
component: BBa_C0012
component: BBa_R0051
component: BBa_R0065
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definition: https://synbiohub.org/public/igem/BBa_B0012/1

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definition: https://synbiohub.org/public/igem/BBa_B0030/1

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definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_R0040
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definition: https://synbiohub.org/public/igem/BBa_R0040/1

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display id: component1003037
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3OC6HSL amplifier + reporter device
creator: Jeffrey J Tabor
created: 2005-09-06T11:00:00Z
modified: 2015-08-31T04:07:39Z
discontinued: false
dominant: false
group u list: _37_5_
m user id: 0
owner id: 88
owning group id: 37
sample status: Not in stock
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description: Olmecs: Synchro-Dualator part 1
creator: Olmecs 2003 IAP Team
created: 2003-11-27T12:00:00Z
modified: 2015-08-31T04:07:42Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
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sample status: For reference only
status: status/Informational
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Test: Tar-EnvZ attractant receiver (I3302.E0422)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-28T12:00:00Z
modified: 2015-08-31T04:07:41Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Tests Tar-EnvZ attractant receiver device (ompF upstream version) by attaching TIPS output to CFP reporter.
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: QPI Test Construct Intermediate (Q04121.E0430)
creator: Barry Canton
created: 2004-07-13T11:00:00Z
modified: 2015-08-31T04:07:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 159
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
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owned by: myers
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AspA attractant sender device (B0034.C0083)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-28T12:00:00Z
modified: 2015-08-31T04:07:41Z
discontinued: false
dominant: true
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: TIPS in, AspA enzyme out
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owned by: myers
star: false
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role: SO:0000804
role: partType/Project
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component: BBa_C0083
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Test: Switch response to aspartate input (I3410.E0422.I3411.E0432.I3100.I3400.I3302.I3400.I3102.I340
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-28T12:00:00Z
modified: 2015-08-31T04:07:41Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Tests ability of switch to respond to aspartate input by connecting the Tar-EnvZ receptor (I3302 ompF upstream version) to one of the switch inputs. The remaining testbed is analogous to the switch functionality testbed (I3420).
mutable notes: The layout of the switch mimics the layout of the proposed Polkadorks E. colibrator (I3000). Two switch outputs are insulated by appearing first. Hk022cI is attached to the CFP reporter (I3410.E0422). Lambda cI is attached to the YFP reporter (I3411.E0432). The three individually controlled trigger inputs follow the outputs in the layout. Lac trigger controls one hk022cI input (I3100.I3400), and Tar-EnvZ receptor (I3302 upstream ompF version) controls the other (I3302.I3400). The dual control forms the built-in OR gate in one of the switch inputs. Finally, AraC trigger controls the lambda cI input (I3102.I3401).
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description: Tar-EnvZ attractant receiver device (R1075.B0034.C0082.B0015.R0083)
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description: 3OC6HSL + LuxR reporter and aiia (LVA+) tuning device
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Prakash: Segment 2
creator: Randy Rettberg
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modified: 2015-08-31T04:07:41Z
discontinued: false
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display id: BBa_I2021
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description: Constitutive Promoter II Test (R1075.E0422)
creator: Alvin Carter Powers, Chris Walsh (Fighting Darwins)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:39Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: tests the strength of R1075, indicated by reporter expression
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description: intermediate for lac relaxation oscillator
creator: jasonk
created: 2004-10-04T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
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part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
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description: RhlR, LuxR, and LasR constitutive generation (using TetR)
creator: Ronny Krashinsky
created: 2004-01-31T12:00:00Z
modified: 2015-08-31T04:08:11Z
discontinued: false
dominant: false
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description: Booperator test: RhlR biasing, RhlR/LuxR/LasR crosstalk
creator: Ronny Krashinsky
created: 2004-01-31T12:00:00Z
modified: 2015-08-31T04:08:11Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
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sample status: Not in stock
status: status/Unavailable
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive Promoter I Test (R0074.E0422)
creator: Sara Neves, Chris Walsh (Fighting Darwins)
created: 2004-01-28T12:00:00Z
modified: 2015-08-31T04:07:39Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: tests the strength of R0074, indicated by strength of expression of reporter
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type: DnaRegion
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component: BBa_E0022
component: BBa_B0010
component: BBa_R0074
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sequence annotation: BBa_R0074
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sender (cinI) B0034.C0076
creator: crackdots
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:08:11Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
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owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0034
component: BBa_C0076
sequence annotation: BBa_C0076
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definition: https://synbiohub.org/public/igem/BBa_B0034/1

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definition: https://synbiohub.org/public/igem/BBa_C0076/1

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display id: BBa_I4201
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Prakash *: Synchronator Design 1
creator: Caitlin Conboy and Jennifer Braff
created: 2003-12-04T12:00:00Z
modified: 2015-08-31T04:07:41Z
discontinued: false
dominant: false
group u list: _1_
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owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
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BBa_I13026
type: ComponentDefinition
persistent identity: BBa I13026
display id: BBa_I13026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mnt (weak) QPI intermediate
creator: jenmitch
created: 2004-07-14T11:00:00Z
modified: 2015-08-31T04:07:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 159
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0034
component: BBa_C0073
sequence annotation: BBa_C0073
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I13026_sequence/1

BBa_B0034
type: Component
persistent identity: component939428
display id: component939428
version: 1
top level: BBa_I13026
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0073
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display id: component939444
version: 1
top level: BBa_I13026
access: public
definition: https://synbiohub.org/public/igem/BBa_C0073/1

BBa_C0073
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display id: annotation939444
version: 1
top level: BBa_I13026
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component: BBa_C0073

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version: 1
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type: ComponentDefinition
persistent identity: BBa I13028
display id: BBa_I13028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cI (TP-901) QPI intermediate
creator: jenmitch
created: 2004-07-14T11:00:00Z
modified: 2015-08-31T04:07:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 159
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_C0075
component: BBa_B0034
sequence annotation: BBa_C0075
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I13028_sequence/1

BBa_C0075
type: Component
persistent identity: component939472
display id: component939472
version: 1
top level: BBa_I13028
access: public
definition: https://synbiohub.org/public/igem/BBa_C0075/1

BBa_B0034
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persistent identity: component939462
display id: component939462
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0075
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version: 1
top level: BBa_I13028
location: https://synbiohub.org/public/igem/BBa_I13028/annotation939472/range939472/1
component: BBa_C0075

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display id: range939472
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start: 19
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type: SequenceAnnotation
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display id: annotation939462
version: 1
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component: BBa_B0034

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persistent identity: range939462
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TSensor
type: ComponentDefinition
persistent identity: BBa J11021
display id: BBa_J11021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tunable Temperature Sensor v2 (using LacI ts + other parts from Registry 7.05)
creator: Emanuel Nazareth
created: 2005-10-03T11:00:00Z
modified: 2015-08-31T04:08:25Z
discontinued: false
dominant: false
group u list: _38_
m user id: 0
owner id: 354
owning group id: 38
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Tuneable Temperature Sensor made with parts available in Registry 7.05 DNA-1. At higher temperatures, the lacI dimer degrades into lacI monomers. The LacI promoter is no longer inhibited, hence more RFP is produced. Also, cI 434 is produced resulting in Prm+ being repressed, thus lowering the levels of GFP. At lower temperatures, the lacI dimer is allowed to form, hence the LacI promoter is repressed. RFP levels decrease, and cI levels drop. This results in Prm+ no longer being repressed, hence GFP levels increase. Behaviour is tuneable by adjusting the levels of lacI ts via L-arabinose activating pBad. The net result is that the fluorescence (green to red) can be correlated to a temperature (at a given level of L-arabinose). Note IPTG induces R0010, offering a second degree of tuning.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0010
component: BBa_E0040
component: BBa_I12006
component: BBa_B0031
component: BBa_B0034
component: BBa_B0012
component: BBa_C0056
component: BBa_R0010
component: BBa_E1010
component: BBa_B0032
component: BBa_J06501
component: BBa_I0500
sequence annotation: BBa_B0012
sequence annotation: BBa_R0010
sequence annotation: BBa_B0032
sequence annotation: BBa_B0031
sequence annotation: BBa_B0034
sequence annotation: BBa_J06501
sequence annotation: BBa_C0056
sequence annotation: BBa_B0010
sequence annotation: BBa_I12006
sequence annotation: BBa_I0500
sequence annotation: BBa_E1010
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_J11021_sequence/1

BBa_B0010
type: Component
persistent identity: component1717951
display id: component1717951
version: 1
top level: TSensor
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0040
type: Component
persistent identity: component1717946
display id: component1717946
version: 1
top level: TSensor
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_I12006
type: Component
persistent identity: component1717935
display id: component1717935
version: 1
top level: TSensor
access: public
definition: https://synbiohub.org/public/igem/BBa_I12006/1

BBa_B0031
type: Component
persistent identity: component1717990
display id: component1717990
version: 1
top level: TSensor
access: public
definition: https://synbiohub.org/public/igem/BBa_B0031/1

BBa_B0034
type: Component
persistent identity: component1717834
display id: component1717834
version: 1
top level: TSensor
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1717853
display id: component1717853
version: 1
top level: TSensor
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
type: Component
persistent identity: component1718003
display id: component1718003
version: 1
top level: TSensor
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1717863
display id: component1717863
version: 1
top level: TSensor
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0056
type: Component
persistent identity: component1717900
display id: component1717900
version: 1
top level: TSensor
access: public
definition: https://synbiohub.org/public/igem/BBa_C0056/1

BBa_R0010
type: Component
persistent identity: component1717979
display id: component1717979
version: 1
top level: TSensor
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0010
type: Component
persistent identity: component1717905
display id: component1717905
version: 1
top level: TSensor
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E1010
type: Component
persistent identity: component1717997
display id: component1717997
version: 1
top level: TSensor
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_B0031
type: Component
persistent identity: component1717892
display id: component1717892
version: 1
top level: TSensor
access: public
definition: https://synbiohub.org/public/igem/BBa_B0031/1

BBa_B0012
type: Component
persistent identity: component1718013
display id: component1718013
version: 1
top level: TSensor
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0032
type: Component
persistent identity: component1717943
display id: component1717943
version: 1
top level: TSensor
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_B0012
type: Component
persistent identity: component1717961
display id: component1717961
version: 1
top level: TSensor
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_J06501
type: Component
persistent identity: component1717848
display id: component1717848
version: 1
top level: TSensor
access: public
definition: https://synbiohub.org/public/igem/BBa_J06501/1

BBa_R0010
type: Component
persistent identity: component1717881
display id: component1717881
version: 1
top level: TSensor
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0012
type: Component
persistent identity: component1717915
display id: component1717915
version: 1
top level: TSensor
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_I0500
type: Component
persistent identity: component1717829
display id: component1717829
version: 1
top level: TSensor
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1717961
display id: annotation1717961
version: 1
top level: TSensor
location: https://synbiohub.org/public/igem/BBa_J11021/annotation1717961/range1717961/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J11021/annotation1717961/range1717961/1
type: Range
persistent identity: range1717961
display id: range1717961
version: 1
direction: direction/forward
top level: TSensor
start: 4469
end: 4509
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1717979
display id: annotation1717979
version: 1
top level: TSensor
location: https://synbiohub.org/public/igem/BBa_J11021/annotation1717979/range1717979/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_J11021/annotation1717979/range1717979/1
type: Range
persistent identity: range1717979
display id: range1717979
version: 1
direction: direction/forward
top level: TSensor
start: 4518
end: 4717
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1717943
display id: annotation1717943
version: 1
top level: TSensor
location: https://synbiohub.org/public/igem/BBa_J11021/annotation1717943/range1717943/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_J11021/annotation1717943/range1717943/1
type: Range
persistent identity: range1717943
display id: range1717943
version: 1
direction: direction/forward
top level: TSensor
start: 3634
end: 3646
orientation: inline

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation1717892
display id: annotation1717892
version: 1
top level: TSensor
location: https://synbiohub.org/public/igem/BBa_J11021/annotation1717892/range1717892/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_J11021/annotation1717892/range1717892/1
type: Range
persistent identity: range1717892
display id: range1717892
version: 1
direction: direction/forward
top level: TSensor
start: 2743
end: 2756
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1717834
display id: annotation1717834
version: 1
top level: TSensor
location: https://synbiohub.org/public/igem/BBa_J11021/annotation1717834/range1717834/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J11021/annotation1717834/range1717834/1
type: Range
persistent identity: range1717834
display id: range1717834
version: 1
direction: direction/forward
top level: TSensor
start: 1219
end: 1230
orientation: inline

BBa_J06501
type: SequenceAnnotation
persistent identity: annotation1717848
display id: annotation1717848
version: 1
top level: TSensor
location: https://synbiohub.org/public/igem/BBa_J11021/annotation1717848/range1717848/1
component: BBa_J06501

https://synbiohub.org/public/igem/BBa_J11021/annotation1717848/range1717848/1
type: Range
persistent identity: range1717848
display id: range1717848
version: 1
direction: direction/forward
top level: TSensor
start: 1237
end: 2389
orientation: inline

BBa_C0056
type: SequenceAnnotation
persistent identity: annotation1717900
display id: annotation1717900
version: 1
top level: TSensor
location: https://synbiohub.org/public/igem/BBa_J11021/annotation1717900/range1717900/1
component: BBa_C0056

https://synbiohub.org/public/igem/BBa_J11021/annotation1717900/range1717900/1
type: Range
persistent identity: range1717900
display id: range1717900
version: 1
direction: direction/forward
top level: TSensor
start: 2763
end: 3398
orientation: inline

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation1717990
display id: annotation1717990
version: 1
top level: TSensor
location: https://synbiohub.org/public/igem/BBa_J11021/annotation1717990/range1717990/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_J11021/annotation1717990/range1717990/1
type: Range
persistent identity: range1717990
display id: range1717990
version: 1
direction: direction/forward
top level: TSensor
start: 4726
end: 4739
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1718013
display id: annotation1718013
version: 1
top level: TSensor
location: https://synbiohub.org/public/igem/BBa_J11021/annotation1718013/range1718013/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J11021/annotation1718013/range1718013/1
type: Range
persistent identity: range1718013
display id: range1718013
version: 1
direction: direction/forward
top level: TSensor
start: 5548
end: 5588
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1717905
display id: annotation1717905
version: 1
top level: TSensor
location: https://synbiohub.org/public/igem/BBa_J11021/annotation1717905/range1717905/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J11021/annotation1717905/range1717905/1
type: Range
persistent identity: range1717905
display id: range1717905
version: 1
direction: direction/forward
top level: TSensor
start: 3407
end: 3486
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1718003
display id: annotation1718003
version: 1
top level: TSensor
location: https://synbiohub.org/public/igem/BBa_J11021/annotation1718003/range1718003/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J11021/annotation1718003/range1718003/1
type: Range
persistent identity: range1718003
display id: range1718003
version: 1
direction: direction/forward
top level: TSensor
start: 5460
end: 5539
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1717915
display id: annotation1717915
version: 1
top level: TSensor
location: https://synbiohub.org/public/igem/BBa_J11021/annotation1717915/range1717915/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J11021/annotation1717915/range1717915/1
type: Range
persistent identity: range1717915
display id: range1717915
version: 1
direction: direction/forward
top level: TSensor
start: 3495
end: 3535
orientation: inline

BBa_I12006
type: SequenceAnnotation
persistent identity: annotation1717935
display id: annotation1717935
version: 1
top level: TSensor
location: https://synbiohub.org/public/igem/BBa_J11021/annotation1717935/range1717935/1
component: BBa_I12006

https://synbiohub.org/public/igem/BBa_J11021/annotation1717935/range1717935/1
type: Range
persistent identity: range1717935
display id: range1717935
version: 1
direction: direction/forward
top level: TSensor
start: 3544
end: 3625
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1717863
display id: annotation1717863
version: 1
top level: TSensor
location: https://synbiohub.org/public/igem/BBa_J11021/annotation1717863/range1717863/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J11021/annotation1717863/range1717863/1
type: Range
persistent identity: range1717863
display id: range1717863
version: 1
direction: direction/forward
top level: TSensor
start: 2486
end: 2526
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation1717829
display id: annotation1717829
version: 1
top level: TSensor
location: https://synbiohub.org/public/igem/BBa_J11021/annotation1717829/range1717829/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_J11021/annotation1717829/range1717829/1
type: Range
persistent identity: range1717829
display id: range1717829
version: 1
direction: direction/forward
top level: TSensor
start: 1
end: 1210
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1717997
display id: annotation1717997
version: 1
top level: TSensor
location: https://synbiohub.org/public/igem/BBa_J11021/annotation1717997/range1717997/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_J11021/annotation1717997/range1717997/1
type: Range
persistent identity: range1717997
display id: range1717997
version: 1
direction: direction/forward
top level: TSensor
start: 4746
end: 5426
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1717853
display id: annotation1717853
version: 1
top level: TSensor
location: https://synbiohub.org/public/igem/BBa_J11021/annotation1717853/range1717853/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J11021/annotation1717853/range1717853/1
type: Range
persistent identity: range1717853
display id: range1717853
version: 1
direction: direction/forward
top level: TSensor
start: 2398
end: 2477
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1717881
display id: annotation1717881
version: 1
top level: TSensor
location: https://synbiohub.org/public/igem/BBa_J11021/annotation1717881/range1717881/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_J11021/annotation1717881/range1717881/1
type: Range
persistent identity: range1717881
display id: range1717881
version: 1
direction: direction/forward
top level: TSensor
start: 2535
end: 2734
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1717951
display id: annotation1717951
version: 1
top level: TSensor
location: https://synbiohub.org/public/igem/BBa_J11021/annotation1717951/range1717951/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J11021/annotation1717951/range1717951/1
type: Range
persistent identity: range1717951
display id: range1717951
version: 1
direction: direction/forward
top level: TSensor
start: 4381
end: 4460
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1717946
display id: annotation1717946
version: 1
top level: TSensor
location: https://synbiohub.org/public/igem/BBa_J11021/annotation1717946/range1717946/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_J11021/annotation1717946/range1717946/1
type: Range
persistent identity: range1717946
display id: range1717946
version: 1
direction: direction/forward
top level: TSensor
start: 3653
end: 4372
orientation: inline