//classic/generator/uncategorized
type: Collection
persistent identity: classic generator uncategorized
display id: classic_generator_uncategorized
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_I12027
member: BBa_I12022
member: virA
member: BBa_I5100
member: BBa_K325249
member: BBa_K206003
member: BBa_M30003
member: BBa_M30001
member: ccdB
member: BBa_J07083
member: BBa_I12024
member: BBa_I12050
member: BBa_I12227
member: BBa_K389318
member: BBa_J07032
member: BBa_K1379005
member: BBa_K1480003
member: BBa_J13102
member: BBa_K206007
member: BBa_I13971
member: BBa_J13101
member: BBa_K1480004
member: BBa_K325239
member: BBa_I12023
member: BBa_J07085
member: ninabGen
member: BBa_K389307
member: B-carotene
member: BBa_S10000
member: BBa_J07086
member: BBa_K1379007
member: BBa_K325209
member: HydA
member: BBa_J07084
member: BBa_I13972
member: BBa_J07033
member: BBa_K1480005
member: BBa_I12226
member: BBa_M30004
member: BBa_K206002
member: BBa_I13970
member: BBa_K606030
member: BBa_K325259
member: BBa_I15041
member: BBa_I13458
member: BBa_K325229
member: BBa_J07082
member: BBa_K389302
member: BBa_I15023
member: BBa_J07081
member: RBS.TetR
member: BBa_K325218
member: BBa_K206004
member: BBa_K780004
member: BBa_M30002

BBa_J07085
type: ComponentDefinition
persistent identity: BBa J07085
display id: BBa_J07085
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tetR promoter+rbs+scfv-FecA 522-532+rbs+FecI+rbs+FecR
creator: Annie Vo
created: 2005-09-27T11:00:00Z
modified: 2015-08-31T04:08:20Z
discontinued: false
dominant: false
group u list: _21_
m user id: 0
owner id: 353
owning group id: 21
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part expresses scFv-fused FecA' protein, FecI, and FecR' proteins. FecA' aa 522 to 532 are deleted and replaced by scFv. All are under control of tetR promoter.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J07072
component: BBa_J07060
component: BBa_J07068
component: BBa_J13002
sequence annotation: BBa_J07060
sequence annotation: BBa_J07068
sequence annotation: BBa_J07072
sequence annotation: BBa_J13002
sequence: https://synbiohub.org/public/igem/BBa_J07085_sequence/1

BBa_J07072
type: Component
persistent identity: component2227202
display id: component2227202
version: 1
top level: BBa_J07085
access: public
definition: https://synbiohub.org/public/igem/BBa_J07072/1

BBa_J07060
type: Component
persistent identity: component2227184
display id: component2227184
version: 1
top level: BBa_J07085
access: public
definition: https://synbiohub.org/public/igem/BBa_J07060/1

BBa_J07068
type: Component
persistent identity: component2227190
display id: component2227190
version: 1
top level: BBa_J07085
access: public
definition: https://synbiohub.org/public/igem/BBa_J07068/1

BBa_J13002
type: Component
persistent identity: component2227183
display id: component2227183
version: 1
top level: BBa_J07085
access: public
definition: https://synbiohub.org/public/igem/BBa_J13002/1

BBa_J07060
type: SequenceAnnotation
persistent identity: annotation2227184
display id: annotation2227184
version: 1
top level: BBa_J07085
location: https://synbiohub.org/public/igem/BBa_J07085/annotation2227184/range2227184/1
component: BBa_J07060

https://synbiohub.org/public/igem/BBa_J07085/annotation2227184/range2227184/1
type: Range
persistent identity: range2227184
display id: range2227184
version: 1
direction: direction/reverse
top level: BBa_J07085
start: 81
end: 3200
orientation: inline

BBa_J07068
type: SequenceAnnotation
persistent identity: annotation2227190
display id: annotation2227190
version: 1
top level: BBa_J07085
location: https://synbiohub.org/public/igem/BBa_J07085/annotation2227190/range2227190/1
component: BBa_J07068

https://synbiohub.org/public/igem/BBa_J07085/annotation2227190/range2227190/1
type: Range
persistent identity: range2227190
display id: range2227190
version: 1
direction: direction/reverse
top level: BBa_J07085
start: 3209
end: 3757
orientation: inline

BBa_J07072
type: SequenceAnnotation
persistent identity: annotation2227202
display id: annotation2227202
version: 1
top level: BBa_J07085
location: https://synbiohub.org/public/igem/BBa_J07085/annotation2227202/range2227202/1
component: BBa_J07072

https://synbiohub.org/public/igem/BBa_J07085/annotation2227202/range2227202/1
type: Range
persistent identity: range2227202
display id: range2227202
version: 1
direction: direction/reverse
top level: BBa_J07085
start: 3766
end: 4877
orientation: inline

BBa_J13002
type: SequenceAnnotation
persistent identity: annotation2227183
display id: annotation2227183
version: 1
top level: BBa_J07085
location: https://synbiohub.org/public/igem/BBa_J07085/annotation2227183/range2227183/1
component: BBa_J13002

https://synbiohub.org/public/igem/BBa_J07085/annotation2227183/range2227183/1
type: Range
persistent identity: range2227183
display id: range2227183
version: 1
direction: direction/reverse
top level: BBa_J07085
start: 1
end: 74
orientation: inline

BBa_I5100
type: ComponentDefinition
persistent identity: BBa I5100
display id: BBa_I5100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HK022 cI regulatable expression of YFP
creator: Olmecs 2003 IAP Team
created: 2003-11-27T12:00:00Z
modified: 2015-08-31T04:07:42Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0012
component: BBa_B0034
component: BBa_R0050
component: BBa_B0011
component: BBa_E0032
sequence annotation: BBa_R0050
sequence annotation: BBa_B0034
sequence annotation: BBa_E0032
sequence annotation: BBa_B0012
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_I5100_sequence/1

BBa_B0012
type: Component
persistent identity: component947961
display id: component947961
version: 1
top level: BBa_I5100
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component947937
display id: component947937
version: 1
top level: BBa_I5100
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0050
type: Component
persistent identity: component947927
display id: component947927
version: 1
top level: BBa_I5100
access: public
definition: https://synbiohub.org/public/igem/BBa_R0050/1

BBa_B0011
type: Component
persistent identity: component947969
display id: component947969
version: 1
top level: BBa_I5100
access: public
definition: https://synbiohub.org/public/igem/BBa_B0011/1

BBa_E0032
type: Component
persistent identity: component947949
display id: component947949
version: 1
top level: BBa_I5100
access: public
definition: https://synbiohub.org/public/igem/BBa_E0032/1

BBa_R0050
type: SequenceAnnotation
persistent identity: annotation947927
display id: annotation947927
version: 1
top level: BBa_I5100
location: https://synbiohub.org/public/igem/BBa_I5100/annotation947927/range947927/1
component: BBa_R0050

https://synbiohub.org/public/igem/BBa_I5100/annotation947927/range947927/1
type: Range
persistent identity: range947927
display id: range947927
version: 1
direction: direction/forward
top level: BBa_I5100
start: 1
end: 55
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation947937
display id: annotation947937
version: 1
top level: BBa_I5100
location: https://synbiohub.org/public/igem/BBa_I5100/annotation947937/range947937/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I5100/annotation947937/range947937/1
type: Range
persistent identity: range947937
display id: range947937
version: 1
direction: direction/forward
top level: BBa_I5100
start: 64
end: 75
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation947949
display id: annotation947949
version: 1
top level: BBa_I5100
location: https://synbiohub.org/public/igem/BBa_I5100/annotation947949/range947949/1
component: BBa_E0032

https://synbiohub.org/public/igem/BBa_I5100/annotation947949/range947949/1
type: Range
persistent identity: range947949
display id: range947949
version: 1
direction: direction/forward
top level: BBa_I5100
start: 82
end: 843
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation947961
display id: annotation947961
version: 1
top level: BBa_I5100
location: https://synbiohub.org/public/igem/BBa_I5100/annotation947961/range947961/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I5100/annotation947961/range947961/1
type: Range
persistent identity: range947961
display id: range947961
version: 1
direction: direction/forward
top level: BBa_I5100
start: 852
end: 892
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation947969
display id: annotation947969
version: 1
top level: BBa_I5100
location: https://synbiohub.org/public/igem/BBa_I5100/annotation947969/range947969/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_I5100/annotation947969/range947969/1
type: Range
persistent identity: range947969
display id: range947969
version: 1
direction: direction/forward
top level: BBa_I5100
start: 901
end: 946
orientation: inline

BBa_J07033
type: ComponentDefinition
persistent identity: BBa J07033
display id: BBa_J07033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Composite part for FecA' (promoter::rbs::FecA')
creator: Annie
created: 2005-07-18T11:00:00Z
modified: 2015-08-31T04:08:19Z
discontinued: false
dominant: false
experience: experience/None
group u list: _21_
m user id: 0
owner id: 353
owning group id: 21
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part contains a promoter regulated by LacI, a RBS, and coding sequence of FecA' (FecA without PstI site)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_R0040
component: BBa_J07018
component: BBa_B0034
sequence annotation: BBa_R0040
sequence annotation: BBa_J07018
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_J07033_sequence/1

BBa_R0040
type: Component
persistent identity: component1707636
display id: component1707636
version: 1
top level: BBa_J07033
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_J07018
type: Component
persistent identity: component1707646
display id: component1707646
version: 1
top level: BBa_J07033
access: public
definition: https://synbiohub.org/public/igem/BBa_J07018/1

BBa_B0034
type: Component
persistent identity: component1707644
display id: component1707644
version: 1
top level: BBa_J07033
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1707636
display id: annotation1707636
version: 1
top level: BBa_J07033
location: https://synbiohub.org/public/igem/BBa_J07033/annotation1707636/range1707636/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_J07033/annotation1707636/range1707636/1
type: Range
persistent identity: range1707636
display id: range1707636
version: 1
direction: direction/forward
top level: BBa_J07033
start: 1
end: 54
orientation: inline

BBa_J07018
type: SequenceAnnotation
persistent identity: annotation1707646
display id: annotation1707646
version: 1
top level: BBa_J07033
location: https://synbiohub.org/public/igem/BBa_J07033/annotation1707646/range1707646/1
component: BBa_J07018

https://synbiohub.org/public/igem/BBa_J07033/annotation1707646/range1707646/1
type: Range
persistent identity: range1707646
display id: range1707646
version: 1
direction: direction/forward
top level: BBa_J07033
start: 81
end: 2405
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1707644
display id: annotation1707644
version: 1
top level: BBa_J07033
location: https://synbiohub.org/public/igem/BBa_J07033/annotation1707644/range1707644/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J07033/annotation1707644/range1707644/1
type: Range
persistent identity: range1707644
display id: range1707644
version: 1
direction: direction/forward
top level: BBa_J07033
start: 63
end: 74
orientation: inline

BBa_I12226
type: ComponentDefinition
persistent identity: BBa I12226
display id: BBa_I12226
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Generator for wild-type TetR and lambda cI - Gal4 fusion protein
creator: rwald
created: 2004-08-08T11:00:00Z
modified: 2015-08-31T04:07:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 120
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a generator to create both wild-type TetR and lambda cI - Gal4 fusion protein. It allows one PoPS-in to control two biological ANDs.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_I12202
component: BBa_B0040
component: BBa_B0012
component: BBa_B0010
component: BBa_B0032
component: BBa_I12200
sequence annotation: BBa_B0040
sequence annotation: BBa_B0012
sequence annotation: BBa_I12202
sequence annotation: BBa_B0032
sequence annotation: BBa_I12200
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_I12226_sequence/1

BBa_I12202
type: Component
persistent identity: component1042043
display id: component1042043
version: 1
top level: BBa_I12226
access: public
definition: https://synbiohub.org/public/igem/BBa_I12202/1

BBa_B0040
type: Component
persistent identity: component1042027
display id: component1042027
version: 1
top level: BBa_I12226
access: public
definition: https://synbiohub.org/public/igem/BBa_B0040/1

BBa_B0012
type: Component
persistent identity: component1042059
display id: component1042059
version: 1
top level: BBa_I12226
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1042049
display id: component1042049
version: 1
top level: BBa_I12226
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0032
type: Component
persistent identity: component1042003
display id: component1042003
version: 1
top level: BBa_I12226
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_B0032
type: Component
persistent identity: component1042035
display id: component1042035
version: 1
top level: BBa_I12226
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_I12200
type: Component
persistent identity: component1042020
display id: component1042020
version: 1
top level: BBa_I12226
access: public
definition: https://synbiohub.org/public/igem/BBa_I12200/1

BBa_B0040
type: SequenceAnnotation
persistent identity: annotation1042027
display id: annotation1042027
version: 1
top level: BBa_I12226
location: https://synbiohub.org/public/igem/BBa_I12226/annotation1042027/range1042027/1
component: BBa_B0040

https://synbiohub.org/public/igem/BBa_I12226/annotation1042027/range1042027/1
type: Range
persistent identity: range1042027
display id: range1042027
version: 1
direction: direction/forward
top level: BBa_I12226
start: 890
end: 959
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1042059
display id: annotation1042059
version: 1
top level: BBa_I12226
location: https://synbiohub.org/public/igem/BBa_I12226/annotation1042059/range1042059/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I12226/annotation1042059/range1042059/1
type: Range
persistent identity: range1042059
display id: range1042059
version: 1
direction: direction/forward
top level: BBa_I12226
start: 1735
end: 1775
orientation: inline

BBa_I12202
type: SequenceAnnotation
persistent identity: annotation1042043
display id: annotation1042043
version: 1
top level: BBa_I12226
location: https://synbiohub.org/public/igem/BBa_I12226/annotation1042043/range1042043/1
component: BBa_I12202

https://synbiohub.org/public/igem/BBa_I12226/annotation1042043/range1042043/1
type: Range
persistent identity: range1042043
display id: range1042043
version: 1
direction: direction/forward
top level: BBa_I12226
start: 987
end: 1613
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1042003
display id: annotation1042003
version: 1
top level: BBa_I12226
location: https://synbiohub.org/public/igem/BBa_I12226/annotation1042003/range1042003/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_I12226/annotation1042003/range1042003/1
type: Range
persistent identity: range1042003
display id: range1042003
version: 1
direction: direction/forward
top level: BBa_I12226
start: 1
end: 13
orientation: inline

BBa_I12200
type: SequenceAnnotation
persistent identity: annotation1042020
display id: annotation1042020
version: 1
top level: BBa_I12226
location: https://synbiohub.org/public/igem/BBa_I12226/annotation1042020/range1042020/1
component: BBa_I12200

https://synbiohub.org/public/igem/BBa_I12226/annotation1042020/range1042020/1
type: Range
persistent identity: range1042020
display id: range1042020
version: 1
direction: direction/forward
top level: BBa_I12226
start: 20
end: 856
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1042049
display id: annotation1042049
version: 1
top level: BBa_I12226
location: https://synbiohub.org/public/igem/BBa_I12226/annotation1042049/range1042049/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I12226/annotation1042049/range1042049/1
type: Range
persistent identity: range1042049
display id: range1042049
version: 1
direction: direction/forward
top level: BBa_I12226
start: 1647
end: 1726
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1042035
display id: annotation1042035
version: 1
top level: BBa_I12226
location: https://synbiohub.org/public/igem/BBa_I12226/annotation1042035/range1042035/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_I12226/annotation1042035/range1042035/1
type: Range
persistent identity: range1042035
display id: range1042035
version: 1
direction: direction/forward
top level: BBa_I12226
start: 968
end: 980
orientation: inline

HydA
type: ComponentDefinition
persistent identity: BBa M36596
display id: BBa_M36596
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Oxygen-tolerant hydrogenase (with RBS and terminator)
creator: Emma Marriott, Jessica Lam, Tatum Banayat
created: 2013-06-08T11:00:00Z
modified: 2015-05-08T01:14:05Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _848_
m user id: 0
owner id: 17308
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Hydrogenase is an enzyme that catalyzes the reversible oxidation of H2. This part encodes a hydrogenase actuator taken from the large subunit of the marine bacterium Hydrogenovibrius Marinus, which has been shown to be relatively tolerant to oxygen (Kim et. Al, 2011). This part consists of a strong bicistronic design RBS (found under BioFAB ID: pFAB1655), a codon-optimized HmMBH actuator gene taken from the sequence of the large subunit of hydrogenase found in hydrogenovibrius marinus (original sequence found under F2Z6J6 at uniprot.org), and a high efficiency transcriptional terminator (found under BBa_M36281 in the Registry of Standard Biological Parts). The mechanism of the part is relatively straightforward: Once induced with the PoPS signal, the actuator will initiate protein translation and express the hydrogenase enzyme. Our actuator was used in conjunction with a rhamnose sensor (e.g. BBa_M36284).
mutable notes: Considerations of the benefits and convenience of having a bicsistronic design led us to choose this option, in spite of the fact that the bicistronic design is targeted for E. Coli and has not yet been tested in S. Elongatus. Interestingly enough, although the part was originally designed for expression of the hydrogenase protein in cyanobacteria, it has so far only been proved for the expression of hydrogenase protein in E. Coli. In regards to the HmMBH actuator gene, the sequence was optimized to reduce repeats and the number of sites available to restriction enzymes.
mutable provenance: Bicistronic RBS: Mutalik VK, Guimaraes JC, Cambray G, Mai QA, Christoffersen MJ, Martin L, Yua A, Lam C, Rodriguez C, Bennett G, et al. Quantitative estimation of activity and quality for collections of functional genetic elements. Nat. Methods. 2013;10:347-353. BioFAB. (http://io.biofab.org:5000/simple/randomized_bds) Sequence for large subunit Hydrogenase: Nishihara H, Miyata Y, Miyashita Y, Bernhard M, Pohlmann A, et al. Analysis of the molecular species of hydrogenase in the cells of an obligately chemolithoautotrophic, marine hydrogen-oxidizing bacterium, Hydrogenovibrio marinus. Biosci Biotechnol Biochem. 2001;65:2780???2784. UnitPRO (http://www.uniprot.org/uniprot/F2Z6J6). High efficiency transcriptional terminator: Sunil Bodapati, Julia Joung, Daniel Esquivel-Reynoso. Part: BBa_M36281: Hard Information. Registry of Standard Biological Parts. (http://partsregistry.org/cgi/partsdb/part_info.cgi?part_name=BBa%20M36281)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: Hydrogenase Actuator
sequence annotation: BBa_M36595
sequence annotation: BBa_M36281
sequence annotation: BBa_M36596
sequence: https://synbiohub.org/public/igem/BBa_M36596_sequence/1

Hydrogenase Actuator
type: SequenceAnnotation
persistent identity: annotation2329339
display id: annotation2329339
version: 1
top level: HydA
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_M36596/annotation2329339/range2329339/1

https://synbiohub.org/public/igem/BBa_M36596/annotation2329339/range2329339/1
type: Range
persistent identity: range2329339
display id: range2329339
version: 1
direction: direction/forward
top level: HydA
start: 94
end: 1926
orientation: inline

BBa_M36595
type: SequenceAnnotation
persistent identity: annotation2329338
display id: annotation2329338
version: 1
top level: HydA
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_M36596/annotation2329338/range2329338/1

https://synbiohub.org/public/igem/BBa_M36596/annotation2329338/range2329338/1
type: Range
persistent identity: range2329338
display id: range2329338
version: 1
direction: direction/reverse
top level: HydA
start: 1
end: 87
orientation: inline

BBa_M36281
type: SequenceAnnotation
persistent identity: annotation2329341
display id: annotation2329341
version: 1
top level: HydA
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_M36596/annotation2329341/range2329341/1

https://synbiohub.org/public/igem/BBa_M36596/annotation2329341/range2329341/1
type: Range
persistent identity: range2329341
display id: range2329341
version: 1
direction: direction/reverse
top level: HydA
start: 1935
end: 2016
orientation: inline

BBa_M36596
type: SequenceAnnotation
persistent identity: annotation2329340
display id: annotation2329340
version: 1
top level: HydA
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_M36596/annotation2329340/range2329340/1

https://synbiohub.org/public/igem/BBa_M36596/annotation2329340/range2329340/1
type: Range
persistent identity: range2329340
display id: range2329340
version: 1
direction: direction/reverse
top level: HydA
start: 94
end: 1926
orientation: inline

BBa_I12227
type: ComponentDefinition
persistent identity: BBa I12227
display id: BBa_I12227
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Generator for TetR-4C and lambda cI - Gal4 fusion protein
creator: rwald
created: 2004-08-08T11:00:00Z
modified: 2015-08-31T04:07:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 120
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a generator to create both TetR-4C and lambda cI - Gal4 fusion protein. It allows one PoPS-in to control two biological ANDs.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_I12203
component: BBa_I12200
component: BBa_B0034
component: BBa_B0010
component: BBa_B0040
component: BBa_B0012
sequence annotation: BBa_B0040
sequence annotation: BBa_I12200
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_I12203
sequence: https://synbiohub.org/public/igem/BBa_I12227_sequence/1

BBa_I12203
type: Component
persistent identity: component1042269
display id: component1042269
version: 1
top level: BBa_I12227
access: public
definition: https://synbiohub.org/public/igem/BBa_I12203/1

BBa_I12200
type: Component
persistent identity: component1042250
display id: component1042250
version: 1
top level: BBa_I12227
access: public
definition: https://synbiohub.org/public/igem/BBa_I12200/1

BBa_B0034
type: Component
persistent identity: component1042234
display id: component1042234
version: 1
top level: BBa_I12227
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component1042262
display id: component1042262
version: 1
top level: BBa_I12227
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1042275
display id: component1042275
version: 1
top level: BBa_I12227
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0040
type: Component
persistent identity: component1042257
display id: component1042257
version: 1
top level: BBa_I12227
access: public
definition: https://synbiohub.org/public/igem/BBa_B0040/1

BBa_B0012
type: Component
persistent identity: component1042285
display id: component1042285
version: 1
top level: BBa_I12227
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0040
type: SequenceAnnotation
persistent identity: annotation1042257
display id: annotation1042257
version: 1
top level: BBa_I12227
location: https://synbiohub.org/public/igem/BBa_I12227/annotation1042257/range1042257/1
component: BBa_B0040

https://synbiohub.org/public/igem/BBa_I12227/annotation1042257/range1042257/1
type: Range
persistent identity: range1042257
display id: range1042257
version: 1
direction: direction/forward
top level: BBa_I12227
start: 889
end: 958
orientation: inline

BBa_I12200
type: SequenceAnnotation
persistent identity: annotation1042250
display id: annotation1042250
version: 1
top level: BBa_I12227
location: https://synbiohub.org/public/igem/BBa_I12227/annotation1042250/range1042250/1
component: BBa_I12200

https://synbiohub.org/public/igem/BBa_I12227/annotation1042250/range1042250/1
type: Range
persistent identity: range1042250
display id: range1042250
version: 1
direction: direction/forward
top level: BBa_I12227
start: 19
end: 855
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1042275
display id: annotation1042275
version: 1
top level: BBa_I12227
location: https://synbiohub.org/public/igem/BBa_I12227/annotation1042275/range1042275/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I12227/annotation1042275/range1042275/1
type: Range
persistent identity: range1042275
display id: range1042275
version: 1
direction: direction/forward
top level: BBa_I12227
start: 1645
end: 1724
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1042285
display id: annotation1042285
version: 1
top level: BBa_I12227
location: https://synbiohub.org/public/igem/BBa_I12227/annotation1042285/range1042285/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I12227/annotation1042285/range1042285/1
type: Range
persistent identity: range1042285
display id: range1042285
version: 1
direction: direction/forward
top level: BBa_I12227
start: 1733
end: 1773
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1042234
display id: annotation1042234
version: 1
top level: BBa_I12227
location: https://synbiohub.org/public/igem/BBa_I12227/annotation1042234/range1042234/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I12227/annotation1042234/range1042234/1
type: Range
persistent identity: range1042234
display id: range1042234
version: 1
direction: direction/forward
top level: BBa_I12227
start: 1
end: 12
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1042262
display id: annotation1042262
version: 1
top level: BBa_I12227
location: https://synbiohub.org/public/igem/BBa_I12227/annotation1042262/range1042262/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I12227/annotation1042262/range1042262/1
type: Range
persistent identity: range1042262
display id: range1042262
version: 1
direction: direction/forward
top level: BBa_I12227
start: 967
end: 978
orientation: inline

BBa_I12203
type: SequenceAnnotation
persistent identity: annotation1042269
display id: annotation1042269
version: 1
top level: BBa_I12227
location: https://synbiohub.org/public/igem/BBa_I12227/annotation1042269/range1042269/1
component: BBa_I12203

https://synbiohub.org/public/igem/BBa_I12227/annotation1042269/range1042269/1
type: Range
persistent identity: range1042269
display id: range1042269
version: 1
direction: direction/forward
top level: BBa_I12227
start: 985
end: 1611
orientation: inline

BBa_K206004
type: ComponentDefinition
persistent identity: BBa K206004
display id: BBa_K206004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP generator for testing pBAD wt
creator: Amelia Hardjasa
created: 2009-10-17T11:00:00Z
modified: 2015-05-08T01:11:24Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _307_
m user id: 0
owner id: 4172
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is a construct for testing promoter strength by measuring RFP fluorescence.
mutable notes: No special design considerations.
mutable provenance: Assembled from Registry BioBricks.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_I13453
component: BBa_I13507
sequence annotation: BBa_I13453
sequence annotation: BBa_I13507
sequence: https://synbiohub.org/public/igem/BBa_K206004_sequence/1

BBa_I13453
type: Component
persistent identity: component2256290
display id: component2256290
version: 1
top level: BBa_K206004
access: public
definition: https://synbiohub.org/public/igem/BBa_I13453/1

BBa_I13507
type: Component
persistent identity: component2256302
display id: component2256302
version: 1
top level: BBa_K206004
access: public
definition: https://synbiohub.org/public/igem/BBa_I13507/1

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2256290
display id: annotation2256290
version: 1
top level: BBa_K206004
location: https://synbiohub.org/public/igem/BBa_K206004/annotation2256290/range2256290/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K206004/annotation2256290/range2256290/1
type: Range
persistent identity: range2256290
display id: range2256290
version: 1
direction: direction/reverse
top level: BBa_K206004
start: 1
end: 130
orientation: inline

BBa_I13507
type: SequenceAnnotation
persistent identity: annotation2256302
display id: annotation2256302
version: 1
top level: BBa_K206004
location: https://synbiohub.org/public/igem/BBa_K206004/annotation2256302/range2256302/1
component: BBa_I13507

https://synbiohub.org/public/igem/BBa_K206004/annotation2256302/range2256302/1
type: Range
persistent identity: range2256302
display id: range2256302
version: 1
direction: direction/reverse
top level: BBa_K206004
start: 139
end: 999
orientation: inline

BBa_I12027
type: ComponentDefinition
persistent identity: BBa I12027
display id: BBa_I12027
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 434 cI Generator (pBAD/araC promoter)
creator: endelman
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: true
experience: experience/None
group u list: _3_
m user id: 0
owner id: 145
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Addition of arabinose activates expression of 434 cI (LVA+)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_I0500
component: BBa_B0034
component: BBa_C0052
component: BBa_B0010
component: BBa_B0012
sequence annotation: BBa_I0500
sequence annotation: BBa_C0052
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I12027_sequence/1

BBa_I0500
type: Component
persistent identity: component1014420
display id: component1014420
version: 1
top level: BBa_I12027
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0034
type: Component
persistent identity: component1014425
display id: component1014425
version: 1
top level: BBa_I12027
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0052
type: Component
persistent identity: component1014435
display id: component1014435
version: 1
top level: BBa_I12027
access: public
definition: https://synbiohub.org/public/igem/BBa_C0052/1

BBa_B0010
type: Component
persistent identity: component1014442
display id: component1014442
version: 1
top level: BBa_I12027
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1014452
display id: component1014452
version: 1
top level: BBa_I12027
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation1014420
display id: annotation1014420
version: 1
top level: BBa_I12027
location: https://synbiohub.org/public/igem/BBa_I12027/annotation1014420/range1014420/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_I12027/annotation1014420/range1014420/1
type: Range
persistent identity: range1014420
display id: range1014420
version: 1
direction: direction/forward
top level: BBa_I12027
start: 1
end: 1210
orientation: inline

BBa_C0052
type: SequenceAnnotation
persistent identity: annotation1014435
display id: annotation1014435
version: 1
top level: BBa_I12027
location: https://synbiohub.org/public/igem/BBa_I12027/annotation1014435/range1014435/1
component: BBa_C0052

https://synbiohub.org/public/igem/BBa_I12027/annotation1014435/range1014435/1
type: Range
persistent identity: range1014435
display id: range1014435
version: 1
direction: direction/forward
top level: BBa_I12027
start: 1237
end: 1905
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1014425
display id: annotation1014425
version: 1
top level: BBa_I12027
location: https://synbiohub.org/public/igem/BBa_I12027/annotation1014425/range1014425/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I12027/annotation1014425/range1014425/1
type: Range
persistent identity: range1014425
display id: range1014425
version: 1
direction: direction/forward
top level: BBa_I12027
start: 1219
end: 1230
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1014442
display id: annotation1014442
version: 1
top level: BBa_I12027
location: https://synbiohub.org/public/igem/BBa_I12027/annotation1014442/range1014442/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I12027/annotation1014442/range1014442/1
type: Range
persistent identity: range1014442
display id: range1014442
version: 1
direction: direction/forward
top level: BBa_I12027
start: 1939
end: 2018
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1014452
display id: annotation1014452
version: 1
top level: BBa_I12027
location: https://synbiohub.org/public/igem/BBa_I12027/annotation1014452/range1014452/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I12027/annotation1014452/range1014452/1
type: Range
persistent identity: range1014452
display id: range1014452
version: 1
direction: direction/forward
top level: BBa_I12027
start: 2027
end: 2067
orientation: inline

BBa_J07086
type: ComponentDefinition
persistent identity: BBa J07086
display id: BBa_J07086
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tetR promoter+rbs+scfv-FecA 567-576+rbs+FecI+rbs+FecR'+terminator
creator: Annie Vo
created: 2005-09-27T11:00:00Z
modified: 2015-08-31T04:08:20Z
discontinued: false
dominant: false
group u list: _21_
m user id: 0
owner id: 353
owning group id: 21
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part expresses scFv-fused FecA' protein, FecI, and FecR' proteins. FecA' aa 567 to 576 are deleted and replaced by scFv. All are under control of tetR promoter.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J13002
component: BBa_J07068
component: BBa_J07063
component: BBa_J07072
sequence annotation: BBa_J07063
sequence annotation: BBa_J13002
sequence annotation: BBa_J07072
sequence annotation: BBa_J07068
sequence: https://synbiohub.org/public/igem/BBa_J07086_sequence/1

BBa_J13002
type: Component
persistent identity: component2227210
display id: component2227210
version: 1
top level: BBa_J07086
access: public
definition: https://synbiohub.org/public/igem/BBa_J13002/1

BBa_J07068
type: Component
persistent identity: component2227217
display id: component2227217
version: 1
top level: BBa_J07086
access: public
definition: https://synbiohub.org/public/igem/BBa_J07068/1

BBa_J07063
type: Component
persistent identity: component2227211
display id: component2227211
version: 1
top level: BBa_J07086
access: public
definition: https://synbiohub.org/public/igem/BBa_J07063/1

BBa_J07072
type: Component
persistent identity: component2227229
display id: component2227229
version: 1
top level: BBa_J07086
access: public
definition: https://synbiohub.org/public/igem/BBa_J07072/1

BBa_J07063
type: SequenceAnnotation
persistent identity: annotation2227211
display id: annotation2227211
version: 1
top level: BBa_J07086
location: https://synbiohub.org/public/igem/BBa_J07086/annotation2227211/range2227211/1
component: BBa_J07063

https://synbiohub.org/public/igem/BBa_J07086/annotation2227211/range2227211/1
type: Range
persistent identity: range2227211
display id: range2227211
version: 1
direction: direction/reverse
top level: BBa_J07086
start: 81
end: 3197
orientation: inline

BBa_J13002
type: SequenceAnnotation
persistent identity: annotation2227210
display id: annotation2227210
version: 1
top level: BBa_J07086
location: https://synbiohub.org/public/igem/BBa_J07086/annotation2227210/range2227210/1
component: BBa_J13002

https://synbiohub.org/public/igem/BBa_J07086/annotation2227210/range2227210/1
type: Range
persistent identity: range2227210
display id: range2227210
version: 1
direction: direction/reverse
top level: BBa_J07086
start: 1
end: 74
orientation: inline

BBa_J07072
type: SequenceAnnotation
persistent identity: annotation2227229
display id: annotation2227229
version: 1
top level: BBa_J07086
location: https://synbiohub.org/public/igem/BBa_J07086/annotation2227229/range2227229/1
component: BBa_J07072

https://synbiohub.org/public/igem/BBa_J07086/annotation2227229/range2227229/1
type: Range
persistent identity: range2227229
display id: range2227229
version: 1
direction: direction/reverse
top level: BBa_J07086
start: 3763
end: 4874
orientation: inline

BBa_J07068
type: SequenceAnnotation
persistent identity: annotation2227217
display id: annotation2227217
version: 1
top level: BBa_J07086
location: https://synbiohub.org/public/igem/BBa_J07086/annotation2227217/range2227217/1
component: BBa_J07068

https://synbiohub.org/public/igem/BBa_J07086/annotation2227217/range2227217/1
type: Range
persistent identity: range2227217
display id: range2227217
version: 1
direction: direction/reverse
top level: BBa_J07086
start: 3206
end: 3754
orientation: inline

BBa_J07083
type: ComponentDefinition
persistent identity: BBa J07083
display id: BBa_J07083
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tetR promoter+rbs+scfv-FecA 571+rbs+FecI+rbs+FecR'+terminator
creator: Annie Vo
created: 2005-09-27T11:00:00Z
modified: 2015-08-31T04:08:20Z
discontinued: false
dominant: false
group u list: _21_
m user id: 0
owner id: 353
owning group id: 21
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part expresses scFv-fused FecA' protein, FecI, and FecR' proteins. Fusion is at aa 571 of FecA' protein. All are under control of tetR promoter.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J07053
component: BBa_J07072
component: BBa_J13002
component: BBa_J07068
sequence annotation: BBa_J13002
sequence annotation: BBa_J07068
sequence annotation: BBa_J07053
sequence annotation: BBa_J07072
sequence: https://synbiohub.org/public/igem/BBa_J07083_sequence/1

BBa_J07053
type: Component
persistent identity: component2227125
display id: component2227125
version: 1
top level: BBa_J07083
access: public
definition: https://synbiohub.org/public/igem/BBa_J07053/1

BBa_J07072
type: Component
persistent identity: component2227143
display id: component2227143
version: 1
top level: BBa_J07083
access: public
definition: https://synbiohub.org/public/igem/BBa_J07072/1

BBa_J13002
type: Component
persistent identity: component2227124
display id: component2227124
version: 1
top level: BBa_J07083
access: public
definition: https://synbiohub.org/public/igem/BBa_J13002/1

BBa_J07068
type: Component
persistent identity: component2227131
display id: component2227131
version: 1
top level: BBa_J07083
access: public
definition: https://synbiohub.org/public/igem/BBa_J07068/1

BBa_J13002
type: SequenceAnnotation
persistent identity: annotation2227124
display id: annotation2227124
version: 1
top level: BBa_J07083
location: https://synbiohub.org/public/igem/BBa_J07083/annotation2227124/range2227124/1
component: BBa_J13002

https://synbiohub.org/public/igem/BBa_J07083/annotation2227124/range2227124/1
type: Range
persistent identity: range2227124
display id: range2227124
version: 1
direction: direction/reverse
top level: BBa_J07083
start: 1
end: 74
orientation: inline

BBa_J07068
type: SequenceAnnotation
persistent identity: annotation2227131
display id: annotation2227131
version: 1
top level: BBa_J07083
location: https://synbiohub.org/public/igem/BBa_J07083/annotation2227131/range2227131/1
component: BBa_J07068

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type: Range
persistent identity: range2227131
display id: range2227131
version: 1
direction: direction/reverse
top level: BBa_J07083
start: 3236
end: 3784
orientation: inline

BBa_J07053
type: SequenceAnnotation
persistent identity: annotation2227125
display id: annotation2227125
version: 1
top level: BBa_J07083
location: https://synbiohub.org/public/igem/BBa_J07083/annotation2227125/range2227125/1
component: BBa_J07053

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type: Range
persistent identity: range2227125
display id: range2227125
version: 1
direction: direction/reverse
top level: BBa_J07083
start: 81
end: 3227
orientation: inline

BBa_J07072
type: SequenceAnnotation
persistent identity: annotation2227143
display id: annotation2227143
version: 1
top level: BBa_J07083
location: https://synbiohub.org/public/igem/BBa_J07083/annotation2227143/range2227143/1
component: BBa_J07072

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type: Range
persistent identity: range2227143
display id: range2227143
version: 1
direction: direction/reverse
top level: BBa_J07083
start: 3793
end: 4904
orientation: inline

BBa_M30004
type: ComponentDefinition
persistent identity: BBa M30004
display id: BBa_M30004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: -- No description --
creator: natalie kuldell
created: 2005-11-24T12:00:00Z
modified: 2015-05-08T01:13:59Z
discontinued: false
dominant: true
group u list: _45_1_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: lambda cI inverter (Q04510) directing transcription of mRFP (I13507). Constructed with standard assembly except PCR from VF and VR primers was used to generate Q04510 fragment with four-part inverter. Cells carrying M30004 are pink, however because PCR was used for cloning there may be silent mutations in the insert. M30004 is clone #2. A second clone will be saved as M30003.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_B0010
component: BBa_C0051
component: BBa_B0012
component: BBa_E1010
component: BBa_R0051
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_E1010
sequence annotation: BBa_C0051
sequence annotation: BBa_B0010
sequence annotation: BBa_R0051
sequence: https://synbiohub.org/public/igem/BBa_M30004_sequence/1

BBa_B0034
type: Component
persistent identity: component1751424
display id: component1751424
version: 1
top level: BBa_M30004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1751440
display id: component1751440
version: 1
top level: BBa_M30004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_C0051
type: Component
persistent identity: component1751434
display id: component1751434
version: 1
top level: BBa_M30004
access: public
definition: https://synbiohub.org/public/igem/BBa_C0051/1

BBa_B0012
type: Component
persistent identity: component1751496
display id: component1751496
version: 1
top level: BBa_M30004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1751486
display id: component1751486
version: 1
top level: BBa_M30004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1751450
display id: component1751450
version: 1
top level: BBa_M30004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E1010
type: Component
persistent identity: component1751480
display id: component1751480
version: 1
top level: BBa_M30004
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_R0051
type: Component
persistent identity: component1751465
display id: component1751465
version: 1
top level: BBa_M30004
access: public
definition: https://synbiohub.org/public/igem/BBa_R0051/1

BBa_B0034
type: Component
persistent identity: component1751473
display id: component1751473
version: 1
top level: BBa_M30004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1751424
display id: annotation1751424
version: 1
top level: BBa_M30004
location: https://synbiohub.org/public/igem/BBa_M30004/annotation1751424/range1751424/1
component: BBa_B0034

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type: Range
persistent identity: range1751424
display id: range1751424
version: 1
direction: direction/forward
top level: BBa_M30004
start: 1
end: 12
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1751496
display id: annotation1751496
version: 1
top level: BBa_M30004
location: https://synbiohub.org/public/igem/BBa_M30004/annotation1751496/range1751496/1
component: BBa_B0012

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type: Range
persistent identity: range1751496
display id: range1751496
version: 1
direction: direction/forward
top level: BBa_M30004
start: 1816
end: 1856
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1751480
display id: annotation1751480
version: 1
top level: BBa_M30004
location: https://synbiohub.org/public/igem/BBa_M30004/annotation1751480/range1751480/1
component: BBa_E1010

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type: Range
persistent identity: range1751480
display id: range1751480
version: 1
direction: direction/forward
top level: BBa_M30004
start: 1014
end: 1694
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation1751434
display id: annotation1751434
version: 1
top level: BBa_M30004
location: https://synbiohub.org/public/igem/BBa_M30004/annotation1751434/range1751434/1
component: BBa_C0051

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type: Range
persistent identity: range1751434
display id: range1751434
version: 1
direction: direction/forward
top level: BBa_M30004
start: 19
end: 768
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1751450
display id: annotation1751450
version: 1
top level: BBa_M30004
location: https://synbiohub.org/public/igem/BBa_M30004/annotation1751450/range1751450/1
component: BBa_B0012

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type: Range
persistent identity: range1751450
display id: range1751450
version: 1
direction: direction/forward
top level: BBa_M30004
start: 890
end: 930
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1751486
display id: annotation1751486
version: 1
top level: BBa_M30004
location: https://synbiohub.org/public/igem/BBa_M30004/annotation1751486/range1751486/1
component: BBa_B0010

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type: Range
persistent identity: range1751486
display id: range1751486
version: 1
direction: direction/forward
top level: BBa_M30004
start: 1728
end: 1807
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1751473
display id: annotation1751473
version: 1
top level: BBa_M30004
location: https://synbiohub.org/public/igem/BBa_M30004/annotation1751473/range1751473/1
component: BBa_B0034

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type: Range
persistent identity: range1751473
display id: range1751473
version: 1
direction: direction/forward
top level: BBa_M30004
start: 996
end: 1007
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation1751465
display id: annotation1751465
version: 1
top level: BBa_M30004
location: https://synbiohub.org/public/igem/BBa_M30004/annotation1751465/range1751465/1
component: BBa_R0051

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type: Range
persistent identity: range1751465
display id: range1751465
version: 1
direction: direction/forward
top level: BBa_M30004
start: 939
end: 987
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1751440
display id: annotation1751440
version: 1
top level: BBa_M30004
location: https://synbiohub.org/public/igem/BBa_M30004/annotation1751440/range1751440/1
component: BBa_B0010

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type: Range
persistent identity: range1751440
display id: range1751440
version: 1
direction: direction/forward
top level: BBa_M30004
start: 802
end: 881
orientation: inline

BBa_K1379005
type: ComponentDefinition
persistent identity: BBa K1379005
display id: BBa_K1379005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: σx Generator + PcelA-E0240
creator: Jordy Evan, Nadia Benedicta, Angeline Widjaja
created: 2014-09-22T11:00:00Z
modified: 2015-05-08T01:10:12Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1756_
m user id: 0
owner id: 21235
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: SigmaX generator, SigmaX inducible PCelA encoding GFP
mutable notes: N/A
mutable provenance: Both comX gene and PCelA are found in genomic sequence of Streptococcus pneumoniae NCTC7465 strain, while the RBS+GFP+DTT which is downstream of PCelA is BBa_E0240. The promoter encoding comX is BBa_K880005 and the double terminator for it is BBa_B0015
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_E0240
component: BBa_K1379006
component: BBa_K1379000
sequence annotation: BBa_K1379006
sequence annotation: BBa_K1379000
sequence annotation: BBa_E0240
sequence: https://synbiohub.org/public/igem/BBa_K1379005_sequence/1

BBa_E0240
type: Component
persistent identity: component2407450
display id: component2407450
version: 1
top level: BBa_K1379005
access: public
definition: https://synbiohub.org/public/igem/BBa_E0240/1

BBa_K1379006
type: Component
persistent identity: component2407436
display id: component2407436
version: 1
top level: BBa_K1379005
access: public
definition: https://synbiohub.org/public/igem/BBa_K1379006/1

BBa_K1379000
type: Component
persistent identity: component2407439
display id: component2407439
version: 1
top level: BBa_K1379005
access: public
definition: https://synbiohub.org/public/igem/BBa_K1379000/1

BBa_K1379006
type: SequenceAnnotation
persistent identity: annotation2407436
display id: annotation2407436
version: 1
top level: BBa_K1379005
location: https://synbiohub.org/public/igem/BBa_K1379005/annotation2407436/range2407436/1
component: BBa_K1379006

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type: Range
persistent identity: range2407436
display id: range2407436
version: 1
direction: direction/reverse
top level: BBa_K1379005
start: 1
end: 711
orientation: inline

BBa_K1379000
type: SequenceAnnotation
persistent identity: annotation2407439
display id: annotation2407439
version: 1
top level: BBa_K1379005
location: https://synbiohub.org/public/igem/BBa_K1379005/annotation2407439/range2407439/1
component: BBa_K1379000

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type: Range
persistent identity: range2407439
display id: range2407439
version: 1
direction: direction/reverse
top level: BBa_K1379005
start: 720
end: 819
orientation: inline

BBa_E0240
type: SequenceAnnotation
persistent identity: annotation2407450
display id: annotation2407450
version: 1
top level: BBa_K1379005
location: https://synbiohub.org/public/igem/BBa_K1379005/annotation2407450/range2407450/1
component: BBa_E0240

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type: Range
persistent identity: range2407450
display id: range2407450
version: 1
direction: direction/reverse
top level: BBa_K1379005
start: 828
end: 1703
orientation: inline

BBa_I12024
type: ComponentDefinition
persistent identity: BBa I12024
display id: BBa_I12024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lambda cI Generator (PLlac 0-1 promoter )
creator: endelman
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: true
experience: experience/None
group u list: _3_
m user id: 0
owner id: 145
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Expression of Lambda cI (LVA+) can be controlled by IPTG in LacIq strain.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_C0051
component: BBa_B0015
component: BBa_R0011
sequence annotation: BBa_R0011
sequence annotation: BBa_B0015
sequence annotation: BBa_C0051
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I12024_sequence/1

BBa_B0034
type: Component
persistent identity: component2221559
display id: component2221559
version: 1
top level: BBa_I12024
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0051
type: Component
persistent identity: component2221563
display id: component2221563
version: 1
top level: BBa_I12024
access: public
definition: https://synbiohub.org/public/igem/BBa_C0051/1

BBa_B0015
type: Component
persistent identity: component2221570
display id: component2221570
version: 1
top level: BBa_I12024
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_R0011
type: Component
persistent identity: component2221553
display id: component2221553
version: 1
top level: BBa_I12024
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2221553
display id: annotation2221553
version: 1
top level: BBa_I12024
location: https://synbiohub.org/public/igem/BBa_I12024/annotation2221553/range2221553/1
component: BBa_R0011

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type: Range
persistent identity: range2221553
display id: range2221553
version: 1
direction: direction/forward
top level: BBa_I12024
start: 1
end: 54
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2221570
display id: annotation2221570
version: 1
top level: BBa_I12024
location: https://synbiohub.org/public/igem/BBa_I12024/annotation2221570/range2221570/1
component: BBa_B0015

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type: Range
persistent identity: range2221570
display id: range2221570
version: 1
direction: direction/reverse
top level: BBa_I12024
start: 865
end: 993
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation2221563
display id: annotation2221563
version: 1
top level: BBa_I12024
location: https://synbiohub.org/public/igem/BBa_I12024/annotation2221563/range2221563/1
component: BBa_C0051

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type: Range
persistent identity: range2221563
display id: range2221563
version: 1
direction: direction/reverse
top level: BBa_I12024
start: 82
end: 856
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2221559
display id: annotation2221559
version: 1
top level: BBa_I12024
location: https://synbiohub.org/public/igem/BBa_I12024/annotation2221559/range2221559/1
component: BBa_B0034

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type: Range
persistent identity: range2221559
display id: range2221559
version: 1
direction: direction/reverse
top level: BBa_I12024
start: 64
end: 75
orientation: inline

BBa_M30001
type: ComponentDefinition
persistent identity: BBa M30001
display id: BBa_M30001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: -- No description --
creator: natalie kuldell
created: 2005-11-24T12:00:00Z
modified: 2015-05-08T01:13:58Z
discontinued: false
dominant: true
group u list: _45_1_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: P22 c2 inverter (Q04530) directing transcription of mRFP (I13507). Constructed with standard assembly except PCR from VF and VR primers was used to generate Q04530 fragment with four-part inverter. Cells carrying M30001 are red, however because PCR was used for cloning there may be silent mutations in the insert. M30001 is clone #1. A second clone will be saved as M30002.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0012
component: BBa_B0010
component: BBa_C0053
component: BBa_R0053
component: BBa_E1010
component: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_C0053
sequence annotation: BBa_E1010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0053
sequence: https://synbiohub.org/public/igem/BBa_M30001_sequence/1

BBa_B0012
type: Component
persistent identity: component1751078
display id: component1751078
version: 1
top level: BBa_M30001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1751016
display id: component1751016
version: 1
top level: BBa_M30001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_C0053
type: Component
persistent identity: component1751008
display id: component1751008
version: 1
top level: BBa_M30001
access: public
definition: https://synbiohub.org/public/igem/BBa_C0053/1

BBa_R0053
type: Component
persistent identity: component1751045
display id: component1751045
version: 1
top level: BBa_M30001
access: public
definition: https://synbiohub.org/public/igem/BBa_R0053/1

BBa_B0012
type: Component
persistent identity: component1751026
display id: component1751026
version: 1
top level: BBa_M30001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E1010
type: Component
persistent identity: component1751062
display id: component1751062
version: 1
top level: BBa_M30001
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_B0034
type: Component
persistent identity: component1750996
display id: component1750996
version: 1
top level: BBa_M30001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1751068
display id: component1751068
version: 1
top level: BBa_M30001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1751055
display id: component1751055
version: 1
top level: BBa_M30001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1751016
display id: annotation1751016
version: 1
top level: BBa_M30001
location: https://synbiohub.org/public/igem/BBa_M30001/annotation1751016/range1751016/1
component: BBa_B0010

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type: Range
persistent identity: range1751016
display id: range1751016
version: 1
direction: direction/forward
top level: BBa_M30001
start: 739
end: 818
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1750996
display id: annotation1750996
version: 1
top level: BBa_M30001
location: https://synbiohub.org/public/igem/BBa_M30001/annotation1750996/range1750996/1
component: BBa_B0034

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type: Range
persistent identity: range1750996
display id: range1750996
version: 1
direction: direction/forward
top level: BBa_M30001
start: 1
end: 12
orientation: inline

BBa_C0053
type: SequenceAnnotation
persistent identity: annotation1751008
display id: annotation1751008
version: 1
top level: BBa_M30001
location: https://synbiohub.org/public/igem/BBa_M30001/annotation1751008/range1751008/1
component: BBa_C0053

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type: Range
persistent identity: range1751008
display id: range1751008
version: 1
direction: direction/forward
top level: BBa_M30001
start: 19
end: 705
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1751062
display id: annotation1751062
version: 1
top level: BBa_M30001
location: https://synbiohub.org/public/igem/BBa_M30001/annotation1751062/range1751062/1
component: BBa_E1010

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type: Range
persistent identity: range1751062
display id: range1751062
version: 1
direction: direction/forward
top level: BBa_M30001
start: 956
end: 1636
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1751078
display id: annotation1751078
version: 1
top level: BBa_M30001
location: https://synbiohub.org/public/igem/BBa_M30001/annotation1751078/range1751078/1
component: BBa_B0012

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type: Range
persistent identity: range1751078
display id: range1751078
version: 1
direction: direction/forward
top level: BBa_M30001
start: 1758
end: 1798
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1751068
display id: annotation1751068
version: 1
top level: BBa_M30001
location: https://synbiohub.org/public/igem/BBa_M30001/annotation1751068/range1751068/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_M30001/annotation1751068/range1751068/1
type: Range
persistent identity: range1751068
display id: range1751068
version: 1
direction: direction/forward
top level: BBa_M30001
start: 1670
end: 1749
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1751055
display id: annotation1751055
version: 1
top level: BBa_M30001
location: https://synbiohub.org/public/igem/BBa_M30001/annotation1751055/range1751055/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_M30001/annotation1751055/range1751055/1
type: Range
persistent identity: range1751055
display id: range1751055
version: 1
direction: direction/forward
top level: BBa_M30001
start: 938
end: 949
orientation: inline

BBa_R0053
type: SequenceAnnotation
persistent identity: annotation1751045
display id: annotation1751045
version: 1
top level: BBa_M30001
location: https://synbiohub.org/public/igem/BBa_M30001/annotation1751045/range1751045/1
component: BBa_R0053

https://synbiohub.org/public/igem/BBa_M30001/annotation1751045/range1751045/1
type: Range
persistent identity: range1751045
display id: range1751045
version: 1
direction: direction/forward
top level: BBa_M30001
start: 876
end: 929
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1751026
display id: annotation1751026
version: 1
top level: BBa_M30001
location: https://synbiohub.org/public/igem/BBa_M30001/annotation1751026/range1751026/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_M30001/annotation1751026/range1751026/1
type: Range
persistent identity: range1751026
display id: range1751026
version: 1
direction: direction/forward
top level: BBa_M30001
start: 827
end: 867
orientation: inline

BBa_K1379007
type: ComponentDefinition
persistent identity: BBa K1379007
display id: BBa_K1379007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: σx Generator + PcomFA-E0240
creator: Ng Siu Wang Edward, Jason Jang, Joo Min Jung
created: 2014-09-22T11:00:00Z
modified: 2015-05-08T01:10:12Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1756_
m user id: 0
owner id: 21235
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: SigmaX generator, SigmaX-inducible Phelicase encoding GFP
mutable notes: N/A
mutable provenance: Both comX gene and Phelicase are found in genomic sequence of Streptococcus pneumoniae NCTC7465 strain, while the RBS+GFP+DTT, downstream of PCelA, is BBa_E0240. The promoter encoding comX is BBa_K880005 and the double terminator for it is BBa_B0015
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_K1379006
component: BBa_E0240
component: BBa_K1379001
sequence annotation: BBa_K1379001
sequence annotation: BBa_E0240
sequence annotation: BBa_K1379006
sequence: https://synbiohub.org/public/igem/BBa_K1379007_sequence/1

BBa_K1379006
type: Component
persistent identity: component2421710
display id: component2421710
version: 1
top level: BBa_K1379007
access: public
definition: https://synbiohub.org/public/igem/BBa_K1379006/1

BBa_E0240
type: Component
persistent identity: component2421724
display id: component2421724
version: 1
top level: BBa_K1379007
access: public
definition: https://synbiohub.org/public/igem/BBa_E0240/1

BBa_K1379001
type: Component
persistent identity: component2421713
display id: component2421713
version: 1
top level: BBa_K1379007
access: public
definition: https://synbiohub.org/public/igem/BBa_K1379001/1

BBa_K1379001
type: SequenceAnnotation
persistent identity: annotation2421713
display id: annotation2421713
version: 1
top level: BBa_K1379007
location: https://synbiohub.org/public/igem/BBa_K1379007/annotation2421713/range2421713/1
component: BBa_K1379001

https://synbiohub.org/public/igem/BBa_K1379007/annotation2421713/range2421713/1
type: Range
persistent identity: range2421713
display id: range2421713
version: 1
direction: direction/reverse
top level: BBa_K1379007
start: 720
end: 879
orientation: inline

BBa_E0240
type: SequenceAnnotation
persistent identity: annotation2421724
display id: annotation2421724
version: 1
top level: BBa_K1379007
location: https://synbiohub.org/public/igem/BBa_K1379007/annotation2421724/range2421724/1
component: BBa_E0240

https://synbiohub.org/public/igem/BBa_K1379007/annotation2421724/range2421724/1
type: Range
persistent identity: range2421724
display id: range2421724
version: 1
direction: direction/reverse
top level: BBa_K1379007
start: 888
end: 1763
orientation: inline

BBa_K1379006
type: SequenceAnnotation
persistent identity: annotation2421710
display id: annotation2421710
version: 1
top level: BBa_K1379007
location: https://synbiohub.org/public/igem/BBa_K1379007/annotation2421710/range2421710/1
component: BBa_K1379006

https://synbiohub.org/public/igem/BBa_K1379007/annotation2421710/range2421710/1
type: Range
persistent identity: range2421710
display id: range2421710
version: 1
direction: direction/reverse
top level: BBa_K1379007
start: 1
end: 711
orientation: inline

BBa_K325259
type: ComponentDefinition
persistent identity: BBa K325259
display id: BBa_K325259
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Yellow Firefly Luciferase (under pBAD) L. Cruciata (E. coli optimised)
creator: Ben Reeve and Emily Knott
created: 2010-10-22T11:00:00Z
modified: 2015-05-08T01:12:02Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _446_
m user id: 0
owner id: 7466
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: rbs
sequence annotation: AraC
sequence annotation: Pbad
sequence annotation: Yellow Luciferase (452) (L. cruciata)
sequence annotation: PC
sequence annotation: double stop codon
sequence annotation: BamH1 site
sequence annotation: Luciferin Regenerating Enzyme (L. cruciata)
sequence annotation: BBa_I0500
sequence annotation: Pro452Ser
sequence: https://synbiohub.org/public/igem/BBa_K325259_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation2103834
display id: annotation2103834
version: 1
top level: BBa_K325259
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K325259/annotation2103834/range2103834/1

https://synbiohub.org/public/igem/BBa_K325259/annotation2103834/range2103834/1
type: Range
persistent identity: range2103834
display id: range2103834
version: 1
direction: direction/forward
top level: BBa_K325259
start: 2175
end: 2191
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2103829
display id: annotation2103829
version: 1
top level: BBa_K325259
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K325259/annotation2103829/range2103829/1

https://synbiohub.org/public/igem/BBa_K325259/annotation2103829/range2103829/1
type: Range
persistent identity: range2103829
display id: range2103829
version: 1
direction: direction/forward
top level: BBa_K325259
start: 1219
end: 1235
orientation: inline

AraC
type: SequenceAnnotation
persistent identity: annotation2103820
display id: annotation2103820
version: 1
top level: BBa_K325259
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K325259/annotation2103820/range2103820/1

https://synbiohub.org/public/igem/BBa_K325259/annotation2103820/range2103820/1
type: Range
persistent identity: range2103820
display id: range2103820
version: 1
direction: direction/forward
top level: BBa_K325259
start: 1
end: 1079
orientation: inline

Pbad
type: SequenceAnnotation
persistent identity: annotation2103828
display id: annotation2103828
version: 1
top level: BBa_K325259
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K325259/annotation2103828/range2103828/1

https://synbiohub.org/public/igem/BBa_K325259/annotation2103828/range2103828/1
type: Range
persistent identity: range2103828
display id: range2103828
version: 1
direction: direction/forward
top level: BBa_K325259
start: 1080
end: 1210
orientation: inline

Yellow Luciferase (452) (L. cruciata)
type: SequenceAnnotation
persistent identity: annotation2103835
display id: annotation2103835
version: 1
top level: BBa_K325259
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K325259/annotation2103835/range2103835/1

https://synbiohub.org/public/igem/BBa_K325259/annotation2103835/range2103835/1
type: Range
persistent identity: range2103835
display id: range2103835
version: 1
direction: direction/forward
top level: BBa_K325259
start: 2192
end: 3835
orientation: inline

PC
type: SequenceAnnotation
persistent identity: annotation2103821
display id: annotation2103821
version: 1
top level: BBa_K325259
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K325259/annotation2103821/range2103821/1

https://synbiohub.org/public/igem/BBa_K325259/annotation2103821/range2103821/1
type: Range
persistent identity: range2103821
display id: range2103821
version: 1
direction: direction/forward
top level: BBa_K325259
start: 1043
end: 1114
orientation: inline

double stop codon
type: SequenceAnnotation
persistent identity: annotation2103836
display id: annotation2103836
version: 1
top level: BBa_K325259
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K325259/annotation2103836/range2103836/1

https://synbiohub.org/public/igem/BBa_K325259/annotation2103836/range2103836/1
type: Range
persistent identity: range2103836
display id: range2103836
version: 1
direction: direction/forward
top level: BBa_K325259
start: 3836
end: 3841
orientation: inline

double stop codon
type: SequenceAnnotation
persistent identity: annotation2103832
display id: annotation2103832
version: 1
top level: BBa_K325259
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K325259/annotation2103832/range2103832/1

https://synbiohub.org/public/igem/BBa_K325259/annotation2103832/range2103832/1
type: Range
persistent identity: range2103832
display id: range2103832
version: 1
direction: direction/forward
top level: BBa_K325259
start: 2163
end: 2168
orientation: inline

BamH1 site
type: SequenceAnnotation
persistent identity: annotation2103833
display id: annotation2103833
version: 1
top level: BBa_K325259
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K325259/annotation2103833/range2103833/1

https://synbiohub.org/public/igem/BBa_K325259/annotation2103833/range2103833/1
type: Range
persistent identity: range2103833
display id: range2103833
version: 1
direction: direction/forward
top level: BBa_K325259
start: 2169
end: 2174
orientation: inline

Luciferin Regenerating Enzyme (L. cruciata)
type: SequenceAnnotation
persistent identity: annotation2103831
display id: annotation2103831
version: 1
top level: BBa_K325259
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K325259/annotation2103831/range2103831/1

https://synbiohub.org/public/igem/BBa_K325259/annotation2103831/range2103831/1
type: Range
persistent identity: range2103831
display id: range2103831
version: 1
direction: direction/forward
top level: BBa_K325259
start: 1236
end: 2162
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2103819
display id: annotation2103819
version: 1
top level: BBa_K325259
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K325259/annotation2103819/range2103819/1

https://synbiohub.org/public/igem/BBa_K325259/annotation2103819/range2103819/1
type: Range
persistent identity: range2103819
display id: range2103819
version: 1
direction: direction/forward
top level: BBa_K325259
start: 1
end: 1210
orientation: inline

Pro452Ser
type: SequenceAnnotation
persistent identity: annotation2103893
display id: annotation2103893
version: 1
top level: BBa_K325259
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K325259/annotation2103893/range2103893/1

https://synbiohub.org/public/igem/BBa_K325259/annotation2103893/range2103893/1
type: Range
persistent identity: range2103893
display id: range2103893
version: 1
direction: direction/forward
top level: BBa_K325259
start: 3545
end: 3545
orientation: inline

BBa_K206003
type: ComponentDefinition
persistent identity: BBa K206003
display id: BBa_K206003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP generator for testing pBAD weak
creator: Amelia Hardjasa
created: 2009-10-17T11:00:00Z
modified: 2015-05-08T01:11:23Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _307_
m user id: 0
owner id: 4172
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is a construct for testing promoter strength by measuring RFP fluorescence.
mutable notes: No special design considerations.
mutable provenance: Assembled from Registry BioBricks.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K206001
component: BBa_I13507
sequence annotation: BBa_K206001
sequence annotation: BBa_I13507
sequence: https://synbiohub.org/public/igem/BBa_K206003_sequence/1

BBa_K206001
type: Component
persistent identity: component2256277
display id: component2256277
version: 1
top level: BBa_K206003
access: public
definition: https://synbiohub.org/public/igem/BBa_K206001/1

BBa_I13507
type: Component
persistent identity: component2256289
display id: component2256289
version: 1
top level: BBa_K206003
access: public
definition: https://synbiohub.org/public/igem/BBa_I13507/1

BBa_K206001
type: SequenceAnnotation
persistent identity: annotation2256277
display id: annotation2256277
version: 1
top level: BBa_K206003
location: https://synbiohub.org/public/igem/BBa_K206003/annotation2256277/range2256277/1
component: BBa_K206001

https://synbiohub.org/public/igem/BBa_K206003/annotation2256277/range2256277/1
type: Range
persistent identity: range2256277
display id: range2256277
version: 1
direction: direction/reverse
top level: BBa_K206003
start: 1
end: 130
orientation: inline

BBa_I13507
type: SequenceAnnotation
persistent identity: annotation2256289
display id: annotation2256289
version: 1
top level: BBa_K206003
location: https://synbiohub.org/public/igem/BBa_K206003/annotation2256289/range2256289/1
component: BBa_I13507

https://synbiohub.org/public/igem/BBa_K206003/annotation2256289/range2256289/1
type: Range
persistent identity: range2256289
display id: range2256289
version: 1
direction: direction/reverse
top level: BBa_K206003
start: 139
end: 999
orientation: inline

BBa_M30002
type: ComponentDefinition
persistent identity: BBa M30002
display id: BBa_M30002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: -- No description --
creator: natalie kuldell
created: 2005-11-24T12:00:00Z
modified: 2015-05-08T01:13:58Z
discontinued: false
dominant: true
group u list: _45_1_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: P22 c2 inverter (Q04530) directing transcription of mRFP (I13507). Constructed with standard assembly except PCR from VF and VR primers was used to generate Q04530 fragment with four-part inverter. Cells carrying M30002 are red, however because PCR was used for cloning there may be silent mutations in the insert. M30002 is clone #2. A second clone will be saved as M30001.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_B0010
component: BBa_R0053
component: BBa_C0053
component: BBa_B0012
component: BBa_E1010
sequence annotation: BBa_B0010
sequence annotation: BBa_C0053
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_E1010
sequence annotation: BBa_R0053
sequence: https://synbiohub.org/public/igem/BBa_M30002_sequence/1

BBa_B0034
type: Component
persistent identity: component1751176
display id: component1751176
version: 1
top level: BBa_M30002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component1751235
display id: component1751235
version: 1
top level: BBa_M30002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1751196
display id: component1751196
version: 1
top level: BBa_M30002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0053
type: Component
persistent identity: component1751225
display id: component1751225
version: 1
top level: BBa_M30002
access: public
definition: https://synbiohub.org/public/igem/BBa_R0053/1

BBa_C0053
type: Component
persistent identity: component1751188
display id: component1751188
version: 1
top level: BBa_M30002
access: public
definition: https://synbiohub.org/public/igem/BBa_C0053/1

BBa_B0012
type: Component
persistent identity: component1751258
display id: component1751258
version: 1
top level: BBa_M30002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1751248
display id: component1751248
version: 1
top level: BBa_M30002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E1010
type: Component
persistent identity: component1751242
display id: component1751242
version: 1
top level: BBa_M30002
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_B0012
type: Component
persistent identity: component1751206
display id: component1751206
version: 1
top level: BBa_M30002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1751196
display id: annotation1751196
version: 1
top level: BBa_M30002
location: https://synbiohub.org/public/igem/BBa_M30002/annotation1751196/range1751196/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_M30002/annotation1751196/range1751196/1
type: Range
persistent identity: range1751196
display id: range1751196
version: 1
direction: direction/forward
top level: BBa_M30002
start: 739
end: 818
orientation: inline

BBa_C0053
type: SequenceAnnotation
persistent identity: annotation1751188
display id: annotation1751188
version: 1
top level: BBa_M30002
location: https://synbiohub.org/public/igem/BBa_M30002/annotation1751188/range1751188/1
component: BBa_C0053

https://synbiohub.org/public/igem/BBa_M30002/annotation1751188/range1751188/1
type: Range
persistent identity: range1751188
display id: range1751188
version: 1
direction: direction/forward
top level: BBa_M30002
start: 19
end: 705
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1751258
display id: annotation1751258
version: 1
top level: BBa_M30002
location: https://synbiohub.org/public/igem/BBa_M30002/annotation1751258/range1751258/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_M30002/annotation1751258/range1751258/1
type: Range
persistent identity: range1751258
display id: range1751258
version: 1
direction: direction/forward
top level: BBa_M30002
start: 1758
end: 1798
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1751176
display id: annotation1751176
version: 1
top level: BBa_M30002
location: https://synbiohub.org/public/igem/BBa_M30002/annotation1751176/range1751176/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_M30002/annotation1751176/range1751176/1
type: Range
persistent identity: range1751176
display id: range1751176
version: 1
direction: direction/forward
top level: BBa_M30002
start: 1
end: 12
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1751242
display id: annotation1751242
version: 1
top level: BBa_M30002
location: https://synbiohub.org/public/igem/BBa_M30002/annotation1751242/range1751242/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_M30002/annotation1751242/range1751242/1
type: Range
persistent identity: range1751242
display id: range1751242
version: 1
direction: direction/forward
top level: BBa_M30002
start: 956
end: 1636
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1751206
display id: annotation1751206
version: 1
top level: BBa_M30002
location: https://synbiohub.org/public/igem/BBa_M30002/annotation1751206/range1751206/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_M30002/annotation1751206/range1751206/1
type: Range
persistent identity: range1751206
display id: range1751206
version: 1
direction: direction/forward
top level: BBa_M30002
start: 827
end: 867
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1751235
display id: annotation1751235
version: 1
top level: BBa_M30002
location: https://synbiohub.org/public/igem/BBa_M30002/annotation1751235/range1751235/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_M30002/annotation1751235/range1751235/1
type: Range
persistent identity: range1751235
display id: range1751235
version: 1
direction: direction/forward
top level: BBa_M30002
start: 938
end: 949
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1751248
display id: annotation1751248
version: 1
top level: BBa_M30002
location: https://synbiohub.org/public/igem/BBa_M30002/annotation1751248/range1751248/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_M30002/annotation1751248/range1751248/1
type: Range
persistent identity: range1751248
display id: range1751248
version: 1
direction: direction/forward
top level: BBa_M30002
start: 1670
end: 1749
orientation: inline

BBa_R0053
type: SequenceAnnotation
persistent identity: annotation1751225
display id: annotation1751225
version: 1
top level: BBa_M30002
location: https://synbiohub.org/public/igem/BBa_M30002/annotation1751225/range1751225/1
component: BBa_R0053

https://synbiohub.org/public/igem/BBa_M30002/annotation1751225/range1751225/1
type: Range
persistent identity: range1751225
display id: range1751225
version: 1
direction: direction/forward
top level: BBa_M30002
start: 876
end: 929
orientation: inline

BBa_K1480004
type: ComponentDefinition
persistent identity: BBa K1480004
display id: BBa_K1480004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TetR generator (Promoter J23106 )
creator: Jesus Gilberto Rodriguez Ceja
created: 2014-10-13T11:00:00Z
modified: 2015-05-08T01:10:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1860_
m user id: 0
owner id: 12878
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a TetR protein generator regulated by the constitutive promoter J23106. The protein has a LVA tag for degradation.
mutable notes: Based on parts of registry.
mutable provenance: It was assembled using assembly 10.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_C0040
component: BBa_B0010
component: BBa_J23106
component: BBa_B0034
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_J23106
sequence annotation: BBa_C0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1480004_sequence/1

BBa_C0040
type: Component
persistent identity: component2419547
display id: component2419547
version: 1
top level: BBa_K1480004
access: public
definition: https://synbiohub.org/public/igem/BBa_C0040/1

BBa_B0010
type: Component
persistent identity: component2419548
display id: component2419548
version: 1
top level: BBa_K1480004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_J23106
type: Component
persistent identity: component2419541
display id: component2419541
version: 1
top level: BBa_K1480004
access: public
definition: https://synbiohub.org/public/igem/BBa_J23106/1

BBa_B0034
type: Component
persistent identity: component2419543
display id: component2419543
version: 1
top level: BBa_K1480004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component2419550
display id: component2419550
version: 1
top level: BBa_K1480004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2419550
display id: annotation2419550
version: 1
top level: BBa_K1480004
location: https://synbiohub.org/public/igem/BBa_K1480004/annotation2419550/range2419550/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K1480004/annotation2419550/range2419550/1
type: Range
persistent identity: range2419550
display id: range2419550
version: 1
direction: direction/forward
top level: BBa_K1480004
start: 843
end: 883
orientation: inline

BBa_J23106
type: SequenceAnnotation
persistent identity: annotation2419541
display id: annotation2419541
version: 1
top level: BBa_K1480004
location: https://synbiohub.org/public/igem/BBa_K1480004/annotation2419541/range2419541/1
component: BBa_J23106

https://synbiohub.org/public/igem/BBa_K1480004/annotation2419541/range2419541/1
type: Range
persistent identity: range2419541
display id: range2419541
version: 1
direction: direction/reverse
top level: BBa_K1480004
start: 1
end: 35
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2419547
display id: annotation2419547
version: 1
top level: BBa_K1480004
location: https://synbiohub.org/public/igem/BBa_K1480004/annotation2419547/range2419547/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K1480004/annotation2419547/range2419547/1
type: Range
persistent identity: range2419547
display id: range2419547
version: 1
direction: direction/reverse
top level: BBa_K1480004
start: 62
end: 746
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2419548
display id: annotation2419548
version: 1
top level: BBa_K1480004
location: https://synbiohub.org/public/igem/BBa_K1480004/annotation2419548/range2419548/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K1480004/annotation2419548/range2419548/1
type: Range
persistent identity: range2419548
display id: range2419548
version: 1
direction: direction/forward
top level: BBa_K1480004
start: 755
end: 834
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2419543
display id: annotation2419543
version: 1
top level: BBa_K1480004
location: https://synbiohub.org/public/igem/BBa_K1480004/annotation2419543/range2419543/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1480004/annotation2419543/range2419543/1
type: Range
persistent identity: range2419543
display id: range2419543
version: 1
direction: direction/reverse
top level: BBa_K1480004
start: 44
end: 55
orientation: inline

ccdB
type: ComponentDefinition
persistent identity: BBa I1000
display id: BBa_I1000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ccd Gene - Causes cell death
creator: Randy Rettberg
created: 2006-01-04T12:00:00Z
is replaced by: https://synbiohub.org/public/igem/BBa_P1010/1
modified: 2015-08-31T04:07:29Z
discontinued: true
dominant: false
experience: experience/Works
group u list: _41_48_1_
m user id: 0
owner id: 25
owning group id: 1
part status: Released HQ 2013
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: The ccd operon having a constitutive promoter and the ccdB coding region. ccdB protein is lethal in normal cloning strains. This part is used to aid the process of moving a BioBrick part to a new plasmid. A target plasmid carrying P1010 is cut and mixed with the cut insert. Plasmids that recombine are selected against because they kill the cell that picks them up. P1010 is only provided as plasmid or in DB3.1 which is ccdB resistant.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: ccdB (rev)
sequence annotation: ccdA- (rev)
sequence: https://synbiohub.org/public/igem/BBa_I1000_sequence/1

ccdB (rev)
type: SequenceAnnotation
persistent identity: annotation1779554
display id: annotation1779554
version: 1
top level: ccdB
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I1000/annotation1779554/range1779554/1

https://synbiohub.org/public/igem/BBa_I1000/annotation1779554/range1779554/1
type: Range
persistent identity: range1779554
display id: range1779554
version: 1
direction: direction/reverse
top level: ccdB
start: 29
end: 334
orientation: inline

ccdA- (rev)
type: SequenceAnnotation
persistent identity: annotation1779558
display id: annotation1779558
version: 1
top level: ccdB
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I1000/annotation1779558/range1779558/1

https://synbiohub.org/public/igem/BBa_I1000/annotation1779558/range1779558/1
type: Range
persistent identity: range1779558
display id: range1779558
version: 1
direction: direction/reverse
top level: ccdB
start: 336
end: 556
orientation: inline

BBa_I13458
type: ComponentDefinition
persistent identity: BBa I13458
display id: BBa_I13458
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: promoter C and arabinose C operon
creator: Josh Michener
created: 2005-08-16T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: PC and AraC from the AraBAD operator. PC and AraC are on the complementary strand, reading right to left.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence: https://synbiohub.org/public/igem/BBa_I13458_sequence/1

BBa_K325218
type: ComponentDefinition
persistent identity: BBa K325218
display id: BBa_K325218
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Orange Firefly Luciferase (under pBAD) L. Cruciata (E. coli optimised)
creator: Anja Hohmann and Ben Reeve
created: 2010-10-22T11:00:00Z
modified: 2015-05-08T01:12:02Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _446_
m user id: 0
owner id: 7466
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K325211
component: BBa_I0500
sequence annotation: BBa_I0500
sequence annotation: BBa_K325211
sequence: https://synbiohub.org/public/igem/BBa_K325218_sequence/1

BBa_K325211
type: Component
persistent identity: component2101660
display id: component2101660
version: 1
top level: BBa_K325218
access: public
definition: https://synbiohub.org/public/igem/BBa_K325211/1

BBa_I0500
type: Component
persistent identity: component2101656
display id: component2101656
version: 1
top level: BBa_K325218
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2101656
display id: annotation2101656
version: 1
top level: BBa_K325218
location: https://synbiohub.org/public/igem/BBa_K325218/annotation2101656/range2101656/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K325218/annotation2101656/range2101656/1
type: Range
persistent identity: range2101656
display id: range2101656
version: 1
direction: direction/reverse
top level: BBa_K325218
start: 1
end: 1210
orientation: inline

BBa_K325211
type: SequenceAnnotation
persistent identity: annotation2101660
display id: annotation2101660
version: 1
top level: BBa_K325218
location: https://synbiohub.org/public/igem/BBa_K325218/annotation2101660/range2101660/1
component: BBa_K325211

https://synbiohub.org/public/igem/BBa_K325218/annotation2101660/range2101660/1
type: Range
persistent identity: range2101660
display id: range2101660
version: 1
direction: direction/reverse
top level: BBa_K325218
start: 1219
end: 2885
orientation: inline

BBa_I13970
type: ComponentDefinition
persistent identity: BBa I13970
display id: BBa_I13970
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lac CFP Protein Generator (R0011.E0021)
creator: robertb
created: 2004-08-08T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: true
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 159
owning group id: 7
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: A CFP protein generator under control of Lac for use as a negative control with the Synchronator constructs.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0010
component: BBa_R0011
component: BBa_B0034
component: BBa_B0012
component: BBa_E0020
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_E0020
sequence annotation: BBa_R0011
sequence: https://synbiohub.org/public/igem/BBa_I13970_sequence/1

BBa_B0010
type: Component
persistent identity: component1040662
display id: component1040662
version: 1
top level: BBa_I13970
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0011
type: Component
persistent identity: component1040646
display id: component1040646
version: 1
top level: BBa_I13970
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_B0034
type: Component
persistent identity: component1040655
display id: component1040655
version: 1
top level: BBa_I13970
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component1040672
display id: component1040672
version: 1
top level: BBa_I13970
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0020
type: Component
persistent identity: component1040657
display id: component1040657
version: 1
top level: BBa_I13970
access: public
definition: https://synbiohub.org/public/igem/BBa_E0020/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1040672
display id: annotation1040672
version: 1
top level: BBa_I13970
location: https://synbiohub.org/public/igem/BBa_I13970/annotation1040672/range1040672/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13970/annotation1040672/range1040672/1
type: Range
persistent identity: range1040672
display id: range1040672
version: 1
direction: direction/forward
top level: BBa_I13970
start: 900
end: 940
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1040662
display id: annotation1040662
version: 1
top level: BBa_I13970
location: https://synbiohub.org/public/igem/BBa_I13970/annotation1040662/range1040662/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13970/annotation1040662/range1040662/1
type: Range
persistent identity: range1040662
display id: range1040662
version: 1
direction: direction/forward
top level: BBa_I13970
start: 812
end: 891
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1040655
display id: annotation1040655
version: 1
top level: BBa_I13970
location: https://synbiohub.org/public/igem/BBa_I13970/annotation1040655/range1040655/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13970/annotation1040655/range1040655/1
type: Range
persistent identity: range1040655
display id: range1040655
version: 1
direction: direction/forward
top level: BBa_I13970
start: 63
end: 74
orientation: inline

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation1040657
display id: annotation1040657
version: 1
top level: BBa_I13970
location: https://synbiohub.org/public/igem/BBa_I13970/annotation1040657/range1040657/1
component: BBa_E0020

https://synbiohub.org/public/igem/BBa_I13970/annotation1040657/range1040657/1
type: Range
persistent identity: range1040657
display id: range1040657
version: 1
direction: direction/forward
top level: BBa_I13970
start: 81
end: 803
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1040646
display id: annotation1040646
version: 1
top level: BBa_I13970
location: https://synbiohub.org/public/igem/BBa_I13970/annotation1040646/range1040646/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_I13970/annotation1040646/range1040646/1
type: Range
persistent identity: range1040646
display id: range1040646
version: 1
direction: direction/forward
top level: BBa_I13970
start: 1
end: 54
orientation: inline

BBa_K206007
type: ComponentDefinition
persistent identity: BBa K206007
display id: BBa_K206007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP generator for testing pBAD wt
creator: Amelia Hardjasa
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _307_
m user id: 0
owner id: 4172
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is a construct for testing promoter strength by measuring GFP fluorescence.
mutable notes: No special design considerations.
mutable provenance: Assembled from Registry BioBricks.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0012
component: BBa_B0034
component: BBa_J04031
component: BBa_B0010
component: BBa_I13453
sequence annotation: BBa_B0012
sequence annotation: BBa_J04031
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_I13453
sequence: https://synbiohub.org/public/igem/BBa_K206007_sequence/1

BBa_B0012
type: Component
persistent identity: component2054376
display id: component2054376
version: 1
top level: BBa_K206007
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component2054368
display id: component2054368
version: 1
top level: BBa_K206007
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_J04031
type: Component
persistent identity: component2054373
display id: component2054373
version: 1
top level: BBa_K206007
access: public
definition: https://synbiohub.org/public/igem/BBa_J04031/1

BBa_B0010
type: Component
persistent identity: component2054374
display id: component2054374
version: 1
top level: BBa_K206007
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I13453
type: Component
persistent identity: component2054366
display id: component2054366
version: 1
top level: BBa_K206007
access: public
definition: https://synbiohub.org/public/igem/BBa_I13453/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2054376
display id: annotation2054376
version: 1
top level: BBa_K206007
location: https://synbiohub.org/public/igem/BBa_K206007/annotation2054376/range2054376/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K206007/annotation2054376/range2054376/1
type: Range
persistent identity: range2054376
display id: range2054376
version: 1
direction: direction/forward
top level: BBa_K206007
start: 1012
end: 1052
orientation: inline

BBa_J04031
type: SequenceAnnotation
persistent identity: annotation2054373
display id: annotation2054373
version: 1
top level: BBa_K206007
location: https://synbiohub.org/public/igem/BBa_K206007/annotation2054373/range2054373/1
component: BBa_J04031

https://synbiohub.org/public/igem/BBa_K206007/annotation2054373/range2054373/1
type: Range
persistent identity: range2054373
display id: range2054373
version: 1
direction: direction/reverse
top level: BBa_K206007
start: 157
end: 915
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2054368
display id: annotation2054368
version: 1
top level: BBa_K206007
location: https://synbiohub.org/public/igem/BBa_K206007/annotation2054368/range2054368/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K206007/annotation2054368/range2054368/1
type: Range
persistent identity: range2054368
display id: range2054368
version: 1
direction: direction/reverse
top level: BBa_K206007
start: 139
end: 150
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2054374
display id: annotation2054374
version: 1
top level: BBa_K206007
location: https://synbiohub.org/public/igem/BBa_K206007/annotation2054374/range2054374/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K206007/annotation2054374/range2054374/1
type: Range
persistent identity: range2054374
display id: range2054374
version: 1
direction: direction/forward
top level: BBa_K206007
start: 924
end: 1003
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2054366
display id: annotation2054366
version: 1
top level: BBa_K206007
location: https://synbiohub.org/public/igem/BBa_K206007/annotation2054366/range2054366/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K206007/annotation2054366/range2054366/1
type: Range
persistent identity: range2054366
display id: range2054366
version: 1
direction: direction/reverse
top level: BBa_K206007
start: 1
end: 130
orientation: inline

BBa_I12050
type: ComponentDefinition
persistent identity: BBa I12050
display id: BBa_I12050
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 434 cI (LVA-) Generator (pBAD/araC promoter)
creator: endelman
created: 2004-09-02T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 145
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Adding arabinose activates expression of 434 cI(LVA-)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0010
component: BBa_I0500
component: BBa_B0012
component: BBa_C0056
component: BBa_B0034
sequence annotation: BBa_C0056
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_I0500
sequence: https://synbiohub.org/public/igem/BBa_I12050_sequence/1

BBa_B0010
type: Component
persistent identity: component1064197
display id: component1064197
version: 1
top level: BBa_I12050
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I0500
type: Component
persistent identity: component1064179
display id: component1064179
version: 1
top level: BBa_I12050
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0012
type: Component
persistent identity: component1064207
display id: component1064207
version: 1
top level: BBa_I12050
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0056
type: Component
persistent identity: component1064192
display id: component1064192
version: 1
top level: BBa_I12050
access: public
definition: https://synbiohub.org/public/igem/BBa_C0056/1

BBa_B0034
type: Component
persistent identity: component1064184
display id: component1064184
version: 1
top level: BBa_I12050
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0056
type: SequenceAnnotation
persistent identity: annotation1064192
display id: annotation1064192
version: 1
top level: BBa_I12050
location: https://synbiohub.org/public/igem/BBa_I12050/annotation1064192/range1064192/1
component: BBa_C0056

https://synbiohub.org/public/igem/BBa_I12050/annotation1064192/range1064192/1
type: Range
persistent identity: range1064192
display id: range1064192
version: 1
direction: direction/forward
top level: BBa_I12050
start: 1237
end: 1872
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1064207
display id: annotation1064207
version: 1
top level: BBa_I12050
location: https://synbiohub.org/public/igem/BBa_I12050/annotation1064207/range1064207/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I12050/annotation1064207/range1064207/1
type: Range
persistent identity: range1064207
display id: range1064207
version: 1
direction: direction/forward
top level: BBa_I12050
start: 1969
end: 2009
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1064197
display id: annotation1064197
version: 1
top level: BBa_I12050
location: https://synbiohub.org/public/igem/BBa_I12050/annotation1064197/range1064197/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I12050/annotation1064197/range1064197/1
type: Range
persistent identity: range1064197
display id: range1064197
version: 1
direction: direction/forward
top level: BBa_I12050
start: 1881
end: 1960
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1064184
display id: annotation1064184
version: 1
top level: BBa_I12050
location: https://synbiohub.org/public/igem/BBa_I12050/annotation1064184/range1064184/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I12050/annotation1064184/range1064184/1
type: Range
persistent identity: range1064184
display id: range1064184
version: 1
direction: direction/forward
top level: BBa_I12050
start: 1219
end: 1230
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation1064179
display id: annotation1064179
version: 1
top level: BBa_I12050
location: https://synbiohub.org/public/igem/BBa_I12050/annotation1064179/range1064179/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_I12050/annotation1064179/range1064179/1
type: Range
persistent identity: range1064179
display id: range1064179
version: 1
direction: direction/forward
top level: BBa_I12050
start: 1
end: 1210
orientation: inline

BBa_K325229
type: ComponentDefinition
persistent identity: BBa K325229
display id: BBa_K325229
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Green Firefly Luciferase and LRE L. Cruciata (E. coli optimised)
creator: Will Handley and Peter Emmrich
created: 2010-10-22T11:00:00Z
modified: 2015-05-08T01:12:02Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _446_
m user id: 0
owner id: 7466
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: double stop codon
sequence annotation: Val239Ile
sequence annotation: PC
sequence annotation: I0500
sequence annotation: Green Luciferase (239) (L. cruciata)
sequence annotation: rbs
sequence annotation: Luciferin Regenerating Enzyme (L. cruciata)
sequence annotation: BamH1 site
sequence annotation: AraC
sequence annotation: Pbad
sequence: https://synbiohub.org/public/igem/BBa_K325229_sequence/1

double stop codon
type: SequenceAnnotation
persistent identity: annotation2103776
display id: annotation2103776
version: 1
top level: BBa_K325229
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K325229/annotation2103776/range2103776/1

https://synbiohub.org/public/igem/BBa_K325229/annotation2103776/range2103776/1
type: Range
persistent identity: range2103776
display id: range2103776
version: 1
direction: direction/forward
top level: BBa_K325229
start: 2163
end: 2168
orientation: inline

Val239Ile
type: SequenceAnnotation
persistent identity: annotation2103875
display id: annotation2103875
version: 1
top level: BBa_K325229
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K325229/annotation2103875/range2103875/1

https://synbiohub.org/public/igem/BBa_K325229/annotation2103875/range2103875/1
type: Range
persistent identity: range2103875
display id: range2103875
version: 1
direction: direction/forward
top level: BBa_K325229
start: 2906
end: 2906
orientation: inline

PC
type: SequenceAnnotation
persistent identity: annotation2103772
display id: annotation2103772
version: 1
top level: BBa_K325229
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K325229/annotation2103772/range2103772/1

https://synbiohub.org/public/igem/BBa_K325229/annotation2103772/range2103772/1
type: Range
persistent identity: range2103772
display id: range2103772
version: 1
direction: direction/forward
top level: BBa_K325229
start: 1043
end: 1114
orientation: inline

I0500
type: SequenceAnnotation
persistent identity: annotation2103771
display id: annotation2103771
version: 1
top level: BBa_K325229
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K325229/annotation2103771/range2103771/1

https://synbiohub.org/public/igem/BBa_K325229/annotation2103771/range2103771/1
type: Range
persistent identity: range2103771
display id: range2103771
version: 1
direction: direction/forward
top level: BBa_K325229
start: 1
end: 1210
orientation: inline

Green Luciferase (239) (L. cruciata)
type: SequenceAnnotation
persistent identity: annotation2103779
display id: annotation2103779
version: 1
top level: BBa_K325229
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K325229/annotation2103779/range2103779/1

https://synbiohub.org/public/igem/BBa_K325229/annotation2103779/range2103779/1
type: Range
persistent identity: range2103779
display id: range2103779
version: 1
direction: direction/forward
top level: BBa_K325229
start: 2192
end: 3835
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2103774
display id: annotation2103774
version: 1
top level: BBa_K325229
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K325229/annotation2103774/range2103774/1

https://synbiohub.org/public/igem/BBa_K325229/annotation2103774/range2103774/1
type: Range
persistent identity: range2103774
display id: range2103774
version: 1
direction: direction/forward
top level: BBa_K325229
start: 1219
end: 1235
orientation: inline

Luciferin Regenerating Enzyme (L. cruciata)
type: SequenceAnnotation
persistent identity: annotation2103775
display id: annotation2103775
version: 1
top level: BBa_K325229
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K325229/annotation2103775/range2103775/1

https://synbiohub.org/public/igem/BBa_K325229/annotation2103775/range2103775/1
type: Range
persistent identity: range2103775
display id: range2103775
version: 1
direction: direction/forward
top level: BBa_K325229
start: 1236
end: 2162
orientation: inline

BamH1 site
type: SequenceAnnotation
persistent identity: annotation2103777
display id: annotation2103777
version: 1
top level: BBa_K325229
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K325229/annotation2103777/range2103777/1

https://synbiohub.org/public/igem/BBa_K325229/annotation2103777/range2103777/1
type: Range
persistent identity: range2103777
display id: range2103777
version: 1
direction: direction/forward
top level: BBa_K325229
start: 2169
end: 2174
orientation: inline

AraC
type: SequenceAnnotation
persistent identity: annotation2103770
display id: annotation2103770
version: 1
top level: BBa_K325229
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K325229/annotation2103770/range2103770/1

https://synbiohub.org/public/igem/BBa_K325229/annotation2103770/range2103770/1
type: Range
persistent identity: range2103770
display id: range2103770
version: 1
direction: direction/forward
top level: BBa_K325229
start: 1
end: 1079
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2103778
display id: annotation2103778
version: 1
top level: BBa_K325229
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K325229/annotation2103778/range2103778/1

https://synbiohub.org/public/igem/BBa_K325229/annotation2103778/range2103778/1
type: Range
persistent identity: range2103778
display id: range2103778
version: 1
direction: direction/forward
top level: BBa_K325229
start: 2175
end: 2191
orientation: inline

double stop codon
type: SequenceAnnotation
persistent identity: annotation2103780
display id: annotation2103780
version: 1
top level: BBa_K325229
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K325229/annotation2103780/range2103780/1

https://synbiohub.org/public/igem/BBa_K325229/annotation2103780/range2103780/1
type: Range
persistent identity: range2103780
display id: range2103780
version: 1
direction: direction/forward
top level: BBa_K325229
start: 3836
end: 3841
orientation: inline

Pbad
type: SequenceAnnotation
persistent identity: annotation2103773
display id: annotation2103773
version: 1
top level: BBa_K325229
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K325229/annotation2103773/range2103773/1

https://synbiohub.org/public/igem/BBa_K325229/annotation2103773/range2103773/1
type: Range
persistent identity: range2103773
display id: range2103773
version: 1
direction: direction/forward
top level: BBa_K325229
start: 1080
end: 1210
orientation: inline

ccdB
type: ComponentDefinition
persistent identity: BBa P1016
display id: BBa_P1016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ccdB cassette without ccdA-
creator: Reshma Shetty
created: 2007-01-29T12:00:00Z
modified: 2015-06-15T01:13:47Z
discontinued: true
dominant: false
experience: experience/Works
group u list: _41_
m user id: 4206
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Deleted
bookmark: false
mutable description: This part is a differs from BBa_P1011 in that the mutations introduced in BBa_P1011 to remove restriction sites are not present in this part. It does have the "normal" ccd promoter and ccdB coding region. It also has a double TAA stop codon as per BioBrick conventions.
mutable notes: ccdA- was eliminated since it is an inactive form of ccdA and should not be required for the toxic phenotype of ccdB.
mutable provenance: This part is derived from BBa_P1010 but it lacks ccdA-.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: -35
sequence annotation: -10
sequence annotation: ccdB
sequence: https://synbiohub.org/public/igem/BBa_P1016_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1910684
display id: annotation1910684
version: 1
top level: ccdB
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_P1016/annotation1910684/range1910684/1

https://synbiohub.org/public/igem/BBa_P1016/annotation1910684/range1910684/1
type: Range
persistent identity: range1910684
display id: range1910684
version: 1
direction: direction/forward
top level: ccdB
start: 41
end: 46
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1910685
display id: annotation1910685
version: 1
top level: ccdB
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_P1016/annotation1910685/range1910685/1

https://synbiohub.org/public/igem/BBa_P1016/annotation1910685/range1910685/1
type: Range
persistent identity: range1910685
display id: range1910685
version: 1
direction: direction/forward
top level: ccdB
start: 64
end: 69
orientation: inline

ccdB
type: SequenceAnnotation
persistent identity: annotation1910686
display id: annotation1910686
version: 1
top level: ccdB
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_P1016/annotation1910686/range1910686/1

https://synbiohub.org/public/igem/BBa_P1016/annotation1910686/range1910686/1
type: Range
persistent identity: range1910686
display id: range1910686
version: 1
direction: direction/forward
top level: ccdB
start: 108
end: 416
orientation: inline

BBa_J07081
type: ComponentDefinition
persistent identity: BBa J07081
display id: BBa_J07081
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tetR promoter+rbs+scfv-FecA 527+rbs+FecI+rbs+FecR'+terminator
creator: Annie Vo
created: 2005-09-27T11:00:00Z
modified: 2015-08-31T04:08:20Z
discontinued: false
dominant: false
group u list: _21_
m user id: 0
owner id: 353
owning group id: 21
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part expresses scFv-fused FecA' protein, FecI, and FecR' protein. Fusion is made at aa 527 of FecA' protein. All are control of tetR promoter.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J07052
component: BBa_J07072
component: BBa_J07068
component: BBa_J13002
sequence annotation: BBa_J13002
sequence annotation: BBa_J07052
sequence annotation: BBa_J07068
sequence annotation: BBa_J07072
sequence: https://synbiohub.org/public/igem/BBa_J07081_sequence/1

BBa_J07052
type: Component
persistent identity: component2227066
display id: component2227066
version: 1
top level: BBa_J07081
access: public
definition: https://synbiohub.org/public/igem/BBa_J07052/1

BBa_J07072
type: Component
persistent identity: component2227084
display id: component2227084
version: 1
top level: BBa_J07081
access: public
definition: https://synbiohub.org/public/igem/BBa_J07072/1

BBa_J07068
type: Component
persistent identity: component2227072
display id: component2227072
version: 1
top level: BBa_J07081
access: public
definition: https://synbiohub.org/public/igem/BBa_J07068/1

BBa_J13002
type: Component
persistent identity: component2227065
display id: component2227065
version: 1
top level: BBa_J07081
access: public
definition: https://synbiohub.org/public/igem/BBa_J13002/1

BBa_J13002
type: SequenceAnnotation
persistent identity: annotation2227065
display id: annotation2227065
version: 1
top level: BBa_J07081
location: https://synbiohub.org/public/igem/BBa_J07081/annotation2227065/range2227065/1
component: BBa_J13002

https://synbiohub.org/public/igem/BBa_J07081/annotation2227065/range2227065/1
type: Range
persistent identity: range2227065
display id: range2227065
version: 1
direction: direction/reverse
top level: BBa_J07081
start: 1
end: 74
orientation: inline

BBa_J07052
type: SequenceAnnotation
persistent identity: annotation2227066
display id: annotation2227066
version: 1
top level: BBa_J07081
location: https://synbiohub.org/public/igem/BBa_J07081/annotation2227066/range2227066/1
component: BBa_J07052

https://synbiohub.org/public/igem/BBa_J07081/annotation2227066/range2227066/1
type: Range
persistent identity: range2227066
display id: range2227066
version: 1
direction: direction/reverse
top level: BBa_J07081
start: 81
end: 3227
orientation: inline

BBa_J07068
type: SequenceAnnotation
persistent identity: annotation2227072
display id: annotation2227072
version: 1
top level: BBa_J07081
location: https://synbiohub.org/public/igem/BBa_J07081/annotation2227072/range2227072/1
component: BBa_J07068

https://synbiohub.org/public/igem/BBa_J07081/annotation2227072/range2227072/1
type: Range
persistent identity: range2227072
display id: range2227072
version: 1
direction: direction/reverse
top level: BBa_J07081
start: 3236
end: 3784
orientation: inline

BBa_J07072
type: SequenceAnnotation
persistent identity: annotation2227084
display id: annotation2227084
version: 1
top level: BBa_J07081
location: https://synbiohub.org/public/igem/BBa_J07081/annotation2227084/range2227084/1
component: BBa_J07072

https://synbiohub.org/public/igem/BBa_J07081/annotation2227084/range2227084/1
type: Range
persistent identity: range2227084
display id: range2227084
version: 1
direction: direction/reverse
top level: BBa_J07081
start: 3793
end: 4904
orientation: inline

BBa_K325249
type: ComponentDefinition
persistent identity: BBa K325249
display id: BBa_K325249
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Scarlet Firefly Luciferase (under pBAD) L. Cruciata (E. coli optimised)
creator: Paul Masset and Bill Collins
created: 2010-10-22T11:00:00Z
modified: 2015-05-08T01:12:02Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _446_
m user id: 0
owner id: 7466
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: BamH1 site
sequence annotation: AraC
sequence annotation: double stop codon
sequence annotation: Pbad
sequence annotation: rbs
sequence annotation: His433Tyr
sequence annotation: BBa_I0500
sequence annotation: PC
sequence annotation: Scarlet Luciferase (433) (L. cruciata)
sequence annotation: Luciferin Regenerating Enzyme (L. cruciata)
sequence: https://synbiohub.org/public/igem/BBa_K325249_sequence/1

BamH1 site
type: SequenceAnnotation
persistent identity: annotation2103808
display id: annotation2103808
version: 1
top level: BBa_K325249
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K325249/annotation2103808/range2103808/1

https://synbiohub.org/public/igem/BBa_K325249/annotation2103808/range2103808/1
type: Range
persistent identity: range2103808
display id: range2103808
version: 1
direction: direction/forward
top level: BBa_K325249
start: 2169
end: 2174
orientation: inline

AraC
type: SequenceAnnotation
persistent identity: annotation2103797
display id: annotation2103797
version: 1
top level: BBa_K325249
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K325249/annotation2103797/range2103797/1

https://synbiohub.org/public/igem/BBa_K325249/annotation2103797/range2103797/1
type: Range
persistent identity: range2103797
display id: range2103797
version: 1
direction: direction/forward
top level: BBa_K325249
start: 1
end: 1079
orientation: inline

double stop codon
type: SequenceAnnotation
persistent identity: annotation2103806
display id: annotation2103806
version: 1
top level: BBa_K325249
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K325249/annotation2103806/range2103806/1

https://synbiohub.org/public/igem/BBa_K325249/annotation2103806/range2103806/1
type: Range
persistent identity: range2103806
display id: range2103806
version: 1
direction: direction/forward
top level: BBa_K325249
start: 2163
end: 2168
orientation: inline

Pbad
type: SequenceAnnotation
persistent identity: annotation2103800
display id: annotation2103800
version: 1
top level: BBa_K325249
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K325249/annotation2103800/range2103800/1

https://synbiohub.org/public/igem/BBa_K325249/annotation2103800/range2103800/1
type: Range
persistent identity: range2103800
display id: range2103800
version: 1
direction: direction/forward
top level: BBa_K325249
start: 1080
end: 1210
orientation: inline

double stop codon
type: SequenceAnnotation
persistent identity: annotation2103816
display id: annotation2103816
version: 1
top level: BBa_K325249
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K325249/annotation2103816/range2103816/1

https://synbiohub.org/public/igem/BBa_K325249/annotation2103816/range2103816/1
type: Range
persistent identity: range2103816
display id: range2103816
version: 1
direction: direction/forward
top level: BBa_K325249
start: 3836
end: 3841
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2103802
display id: annotation2103802
version: 1
top level: BBa_K325249
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K325249/annotation2103802/range2103802/1

https://synbiohub.org/public/igem/BBa_K325249/annotation2103802/range2103802/1
type: Range
persistent identity: range2103802
display id: range2103802
version: 1
direction: direction/forward
top level: BBa_K325249
start: 1219
end: 1235
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2103812
display id: annotation2103812
version: 1
top level: BBa_K325249
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K325249/annotation2103812/range2103812/1

https://synbiohub.org/public/igem/BBa_K325249/annotation2103812/range2103812/1
type: Range
persistent identity: range2103812
display id: range2103812
version: 1
direction: direction/forward
top level: BBa_K325249
start: 2175
end: 2191
orientation: inline

His433Tyr
type: SequenceAnnotation
persistent identity: annotation2103892
display id: annotation2103892
version: 1
top level: BBa_K325249
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K325249/annotation2103892/range2103892/1

https://synbiohub.org/public/igem/BBa_K325249/annotation2103892/range2103892/1
type: Range
persistent identity: range2103892
display id: range2103892
version: 1
direction: direction/forward
top level: BBa_K325249
start: 3488
end: 3488
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2103796
display id: annotation2103796
version: 1
top level: BBa_K325249
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K325249/annotation2103796/range2103796/1

https://synbiohub.org/public/igem/BBa_K325249/annotation2103796/range2103796/1
type: Range
persistent identity: range2103796
display id: range2103796
version: 1
direction: direction/forward
top level: BBa_K325249
start: 1
end: 1210
orientation: inline

PC
type: SequenceAnnotation
persistent identity: annotation2103798
display id: annotation2103798
version: 1
top level: BBa_K325249
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K325249/annotation2103798/range2103798/1

https://synbiohub.org/public/igem/BBa_K325249/annotation2103798/range2103798/1
type: Range
persistent identity: range2103798
display id: range2103798
version: 1
direction: direction/forward
top level: BBa_K325249
start: 1043
end: 1114
orientation: inline

Scarlet Luciferase (433) (L. cruciata)
type: SequenceAnnotation
persistent identity: annotation2103814
display id: annotation2103814
version: 1
top level: BBa_K325249
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K325249/annotation2103814/range2103814/1

https://synbiohub.org/public/igem/BBa_K325249/annotation2103814/range2103814/1
type: Range
persistent identity: range2103814
display id: range2103814
version: 1
direction: direction/forward
top level: BBa_K325249
start: 2192
end: 3835
orientation: inline

Luciferin Regenerating Enzyme (L. cruciata)
type: SequenceAnnotation
persistent identity: annotation2103803
display id: annotation2103803
version: 1
top level: BBa_K325249
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K325249/annotation2103803/range2103803/1

https://synbiohub.org/public/igem/BBa_K325249/annotation2103803/range2103803/1
type: Range
persistent identity: range2103803
display id: range2103803
version: 1
direction: direction/forward
top level: BBa_K325249
start: 1236
end: 2162
orientation: inline

RBS.TetR
type: ComponentDefinition
persistent identity: BBa I15040
display id: BBa_I15040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS.TetR from pASKIBA3
creator: Jeff Tabor
created: 2005-06-01T11:00:00Z
modified: 2015-08-31T04:07:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _5_
m user id: 0
owner id: 88
owning group id: 37
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The part accepts POPS to produce TetR. This sequence encodes the ribosome binding site and tetracycline repressor protein from the plasmid pASKIBA3.
mutable provenance: pASKIBA3 (IBA biotagnology)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: paskiba3_rbs
sequence annotation: paskiba3_TetR
sequence: https://synbiohub.org/public/igem/BBa_I15040_sequence/1

paskiba3_rbs
type: SequenceAnnotation
persistent identity: annotation1501145
display id: annotation1501145
version: 1
top level: RBS.TetR
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I15040/annotation1501145/range1501145/1

https://synbiohub.org/public/igem/BBa_I15040/annotation1501145/range1501145/1
type: Range
persistent identity: range1501145
display id: range1501145
version: 1
direction: direction/forward
top level: RBS.TetR
start: 1
end: 15
orientation: inline

paskiba3_TetR
type: SequenceAnnotation
persistent identity: annotation1501146
display id: annotation1501146
version: 1
top level: RBS.TetR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I15040/annotation1501146/range1501146/1

https://synbiohub.org/public/igem/BBa_I15040/annotation1501146/range1501146/1
type: Range
persistent identity: range1501146
display id: range1501146
version: 1
direction: direction/forward
top level: RBS.TetR
start: 16
end: 642
orientation: inline

ninabGen
type: ComponentDefinition
persistent identity: BBa K343005
display id: BBa_K343005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Beta-carotene 15
creator: Christian Kurtzhals
created: 2010-09-06T11:00:00Z
modified: 2015-05-08T01:12:08Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _465_
m user id: 0
owner id: 6083
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The Beta-carotene 15'15'-monooxygenase generator from the D. melanogaster ninaB gene, cleaves beta-carotene into two retinal molecules. Used together with BBa_274210 (beta-carotene generator) it should induce retinal biosynthesis in e. coli.
mutable notes: A constitutive sigma-70 promotor was chosen. Terminator is lacking on this part.
mutable provenance: [link:https://dgrc.cgb.indiana.edu/product/View?product=4630 Drosophila genomics ressource centre], Indiana University. Found via [link:http://www.flybase.org flybase], ID: [link:http://flybase.org/reports/FBcl0129473.html FBcl0129473]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_R0040
component: BBa_K343001
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_K343001
sequence: https://synbiohub.org/public/igem/BBa_K343005_sequence/1

BBa_B0034
type: Component
persistent identity: component2079706
display id: component2079706
version: 1
top level: ninabGen
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0040
type: Component
persistent identity: component2079700
display id: component2079700
version: 1
top level: ninabGen
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_K343001
type: Component
persistent identity: component2079710
display id: component2079710
version: 1
top level: ninabGen
access: public
definition: https://synbiohub.org/public/igem/BBa_K343001/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2079700
display id: annotation2079700
version: 1
top level: ninabGen
location: https://synbiohub.org/public/igem/BBa_K343005/annotation2079700/range2079700/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K343005/annotation2079700/range2079700/1
type: Range
persistent identity: range2079700
display id: range2079700
version: 1
direction: direction/forward
top level: ninabGen
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2079706
display id: annotation2079706
version: 1
top level: ninabGen
location: https://synbiohub.org/public/igem/BBa_K343005/annotation2079706/range2079706/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K343005/annotation2079706/range2079706/1
type: Range
persistent identity: range2079706
display id: range2079706
version: 1
direction: direction/reverse
top level: ninabGen
start: 63
end: 74
orientation: inline

BBa_K343001
type: SequenceAnnotation
persistent identity: annotation2079710
display id: annotation2079710
version: 1
top level: ninabGen
location: https://synbiohub.org/public/igem/BBa_K343005/annotation2079710/range2079710/1
component: BBa_K343001

https://synbiohub.org/public/igem/BBa_K343005/annotation2079710/range2079710/1
type: Range
persistent identity: range2079710
display id: range2079710
version: 1
direction: direction/reverse
top level: ninabGen
start: 81
end: 1943
orientation: inline

BBa_S10000
type: ComponentDefinition
persistent identity: BBa S10000
display id: BBa_S10000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CheY under plac
creator: mit
created: 2004-10-16T11:00:00Z
modified: 2015-05-08T01:14:52Z
discontinued: true
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: R0011.B0034.C0020.B0015
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_C0020
component: BBa_B0010
component: BBa_B0012
component: BBa_R0011
component: BBa_B0034
sequence annotation: BBa_C0020
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_R0011
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_S10000_sequence/1

BBa_C0020
type: Component
persistent identity: component1249128
display id: component1249128
version: 1
top level: BBa_S10000
access: public
definition: https://synbiohub.org/public/igem/BBa_C0020/1

BBa_B0010
type: Component
persistent identity: component1249134
display id: component1249134
version: 1
top level: BBa_S10000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1249144
display id: component1249144
version: 1
top level: BBa_S10000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0011
type: Component
persistent identity: component1249115
display id: component1249115
version: 1
top level: BBa_S10000
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_B0034
type: Component
persistent identity: component1249124
display id: component1249124
version: 1
top level: BBa_S10000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0020
type: SequenceAnnotation
persistent identity: annotation1249128
display id: annotation1249128
version: 1
top level: BBa_S10000
location: https://synbiohub.org/public/igem/BBa_S10000/annotation1249128/range1249128/1
component: BBa_C0020

https://synbiohub.org/public/igem/BBa_S10000/annotation1249128/range1249128/1
type: Range
persistent identity: range1249128
display id: range1249128
version: 1
direction: direction/forward
top level: BBa_S10000
start: 81
end: 473
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1249134
display id: annotation1249134
version: 1
top level: BBa_S10000
location: https://synbiohub.org/public/igem/BBa_S10000/annotation1249134/range1249134/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_S10000/annotation1249134/range1249134/1
type: Range
persistent identity: range1249134
display id: range1249134
version: 1
direction: direction/forward
top level: BBa_S10000
start: 507
end: 586
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1249124
display id: annotation1249124
version: 1
top level: BBa_S10000
location: https://synbiohub.org/public/igem/BBa_S10000/annotation1249124/range1249124/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S10000/annotation1249124/range1249124/1
type: Range
persistent identity: range1249124
display id: range1249124
version: 1
direction: direction/forward
top level: BBa_S10000
start: 63
end: 74
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1249115
display id: annotation1249115
version: 1
top level: BBa_S10000
location: https://synbiohub.org/public/igem/BBa_S10000/annotation1249115/range1249115/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_S10000/annotation1249115/range1249115/1
type: Range
persistent identity: range1249115
display id: range1249115
version: 1
direction: direction/forward
top level: BBa_S10000
start: 1
end: 54
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1249144
display id: annotation1249144
version: 1
top level: BBa_S10000
location: https://synbiohub.org/public/igem/BBa_S10000/annotation1249144/range1249144/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_S10000/annotation1249144/range1249144/1
type: Range
persistent identity: range1249144
display id: range1249144
version: 1
direction: direction/forward
top level: BBa_S10000
start: 595
end: 635
orientation: inline

BBa_K606030
type: ComponentDefinition
persistent identity: BBa K606030
display id: BBa_K606030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pveg SpoVG RFPmCherry B0015
creator: Axel S??guret
created: 2011-09-17T11:00:00Z
modified: 2015-05-08T01:12:51Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _778_
m user id: 0
owner id: 8931
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: RFPmcherry expression system in Bacillus subtilis
mutable notes: biobrick assembly
mutable provenance: Bacillus Subtilis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0015
component: BBa_K143053
component: BBa_E1010
sequence annotation: BBa_E1010
sequence annotation: BBa_K143053
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K606030_sequence/1

BBa_B0015
type: Component
persistent identity: component2258760
display id: component2258760
version: 1
top level: BBa_K606030
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K143053
type: Component
persistent identity: component2258750
display id: component2258750
version: 1
top level: BBa_K606030
access: public
definition: https://synbiohub.org/public/igem/BBa_K143053/1

BBa_E1010
type: Component
persistent identity: component2258753
display id: component2258753
version: 1
top level: BBa_K606030
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2258753
display id: annotation2258753
version: 1
top level: BBa_K606030
location: https://synbiohub.org/public/igem/BBa_K606030/annotation2258753/range2258753/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K606030/annotation2258753/range2258753/1
type: Range
persistent identity: range2258753
display id: range2258753
version: 1
direction: direction/reverse
top level: BBa_K606030
start: 124
end: 829
orientation: inline

BBa_K143053
type: SequenceAnnotation
persistent identity: annotation2258750
display id: annotation2258750
version: 1
top level: BBa_K606030
location: https://synbiohub.org/public/igem/BBa_K606030/annotation2258750/range2258750/1
component: BBa_K143053

https://synbiohub.org/public/igem/BBa_K606030/annotation2258750/range2258750/1
type: Range
persistent identity: range2258750
display id: range2258750
version: 1
direction: direction/reverse
top level: BBa_K606030
start: 1
end: 117
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2258760
display id: annotation2258760
version: 1
top level: BBa_K606030
location: https://synbiohub.org/public/igem/BBa_K606030/annotation2258760/range2258760/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K606030/annotation2258760/range2258760/1
type: Range
persistent identity: range2258760
display id: range2258760
version: 1
direction: direction/reverse
top level: BBa_K606030
start: 838
end: 966
orientation: inline

BBa_K1480003
type: ComponentDefinition
persistent identity: BBa K1480003
display id: BBa_K1480003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TetR generator (Promoter J23109)
creator: Jesus Gilberto Rodriguez Ceja
created: 2014-10-13T11:00:00Z
modified: 2015-05-08T01:10:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1860_
m user id: 0
owner id: 12878
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a TetR protein generator regulated by the constitutive promoter J23109. The protein has a LVA tag for degradation.
mutable notes: It was assembled using assembly 10.
mutable provenance: Based on parts of the registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23109
component: BBa_B0012
component: BBa_C0040
component: BBa_B0010
component: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_C0040
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_J23109
sequence: https://synbiohub.org/public/igem/BBa_K1480003_sequence/1

BBa_J23109
type: Component
persistent identity: component2419528
display id: component2419528
version: 1
top level: BBa_K1480003
access: public
definition: https://synbiohub.org/public/igem/BBa_J23109/1

BBa_B0012
type: Component
persistent identity: component2419537
display id: component2419537
version: 1
top level: BBa_K1480003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0040
type: Component
persistent identity: component2419534
display id: component2419534
version: 1
top level: BBa_K1480003
access: public
definition: https://synbiohub.org/public/igem/BBa_C0040/1

BBa_B0010
type: Component
persistent identity: component2419535
display id: component2419535
version: 1
top level: BBa_K1480003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component2419530
display id: component2419530
version: 1
top level: BBa_K1480003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2419537
display id: annotation2419537
version: 1
top level: BBa_K1480003
location: https://synbiohub.org/public/igem/BBa_K1480003/annotation2419537/range2419537/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K1480003/annotation2419537/range2419537/1
type: Range
persistent identity: range2419537
display id: range2419537
version: 1
direction: direction/forward
top level: BBa_K1480003
start: 843
end: 883
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2419534
display id: annotation2419534
version: 1
top level: BBa_K1480003
location: https://synbiohub.org/public/igem/BBa_K1480003/annotation2419534/range2419534/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K1480003/annotation2419534/range2419534/1
type: Range
persistent identity: range2419534
display id: range2419534
version: 1
direction: direction/reverse
top level: BBa_K1480003
start: 62
end: 746
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2419530
display id: annotation2419530
version: 1
top level: BBa_K1480003
location: https://synbiohub.org/public/igem/BBa_K1480003/annotation2419530/range2419530/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1480003/annotation2419530/range2419530/1
type: Range
persistent identity: range2419530
display id: range2419530
version: 1
direction: direction/reverse
top level: BBa_K1480003
start: 44
end: 55
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2419535
display id: annotation2419535
version: 1
top level: BBa_K1480003
location: https://synbiohub.org/public/igem/BBa_K1480003/annotation2419535/range2419535/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K1480003/annotation2419535/range2419535/1
type: Range
persistent identity: range2419535
display id: range2419535
version: 1
direction: direction/forward
top level: BBa_K1480003
start: 755
end: 834
orientation: inline

BBa_J23109
type: SequenceAnnotation
persistent identity: annotation2419528
display id: annotation2419528
version: 1
top level: BBa_K1480003
location: https://synbiohub.org/public/igem/BBa_K1480003/annotation2419528/range2419528/1
component: BBa_J23109

https://synbiohub.org/public/igem/BBa_K1480003/annotation2419528/range2419528/1
type: Range
persistent identity: range2419528
display id: range2419528
version: 1
direction: direction/reverse
top level: BBa_K1480003
start: 1
end: 35
orientation: inline

BBa_I12022
type: ComponentDefinition
persistent identity: BBa I12022
display id: BBa_I12022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Inducible 434 cI Repressor Generator (Controlled by IPTG and LacI)
creator: ryhsiao
created: 2004-07-25T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 148
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part will produce 434 cI Repressor when IPTG is introduced into the system. The system must also be free of p22 cII for 434 cI repressor to be expressed.
mutable notes: The absence of p22 cII (constitutive promoter) will yield a constant promotion of LacI, which inhibits the expression of lambda cI. Once IPTG is introduced into the system, it will bind to the LacI, resulting in expression/promotion of 434 cI repressor via the QPI (Quad Part Inverter). Hence, a switch controlled by IPTG is created.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_C0052
component: BBa_B0015
component: BBa_R0053
component: BBa_B0034
component: BBa_Q04121
sequence annotation: BBa_B0034
sequence annotation: BBa_Q04121
sequence annotation: BBa_R0053
sequence annotation: BBa_C0052
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_I12022_sequence/1

BBa_C0052
type: Component
persistent identity: component2229445
display id: component2229445
version: 1
top level: BBa_I12022
access: public
definition: https://synbiohub.org/public/igem/BBa_C0052/1

BBa_B0015
type: Component
persistent identity: component2229454
display id: component2229454
version: 1
top level: BBa_I12022
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_R0053
type: Component
persistent identity: component2229417
display id: component2229417
version: 1
top level: BBa_I12022
access: public
definition: https://synbiohub.org/public/igem/BBa_R0053/1

BBa_B0034
type: Component
persistent identity: component2229443
display id: component2229443
version: 1
top level: BBa_I12022
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_Q04121
type: Component
persistent identity: component2229441
display id: component2229441
version: 1
top level: BBa_I12022
access: public
definition: https://synbiohub.org/public/igem/BBa_Q04121/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2229443
display id: annotation2229443
version: 1
top level: BBa_I12022
location: https://synbiohub.org/public/igem/BBa_I12022/annotation2229443/range2229443/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I12022/annotation2229443/range2229443/1
type: Range
persistent identity: range2229443
display id: range2229443
version: 1
direction: direction/reverse
top level: BBa_I12022
start: 1442
end: 1453
orientation: inline

BBa_Q04121
type: SequenceAnnotation
persistent identity: annotation2229441
display id: annotation2229441
version: 1
top level: BBa_I12022
location: https://synbiohub.org/public/igem/BBa_I12022/annotation2229441/range2229441/1
component: BBa_Q04121

https://synbiohub.org/public/igem/BBa_I12022/annotation2229441/range2229441/1
type: Range
persistent identity: range2229441
display id: range2229441
version: 1
direction: direction/reverse
top level: BBa_I12022
start: 63
end: 1433
orientation: inline

BBa_R0053
type: SequenceAnnotation
persistent identity: annotation2229417
display id: annotation2229417
version: 1
top level: BBa_I12022
location: https://synbiohub.org/public/igem/BBa_I12022/annotation2229417/range2229417/1
component: BBa_R0053

https://synbiohub.org/public/igem/BBa_I12022/annotation2229417/range2229417/1
type: Range
persistent identity: range2229417
display id: range2229417
version: 1
direction: direction/forward
top level: BBa_I12022
start: 1
end: 54
orientation: inline

BBa_C0052
type: SequenceAnnotation
persistent identity: annotation2229445
display id: annotation2229445
version: 1
top level: BBa_I12022
location: https://synbiohub.org/public/igem/BBa_I12022/annotation2229445/range2229445/1
component: BBa_C0052

https://synbiohub.org/public/igem/BBa_I12022/annotation2229445/range2229445/1
type: Range
persistent identity: range2229445
display id: range2229445
version: 1
direction: direction/forward
top level: BBa_I12022
start: 1460
end: 2128
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2229454
display id: annotation2229454
version: 1
top level: BBa_I12022
location: https://synbiohub.org/public/igem/BBa_I12022/annotation2229454/range2229454/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_I12022/annotation2229454/range2229454/1
type: Range
persistent identity: range2229454
display id: range2229454
version: 1
direction: direction/reverse
top level: BBa_I12022
start: 2162
end: 2290
orientation: inline

BBa_K389318
type: ComponentDefinition
persistent identity: BBa K389318
display id: BBa_K389318
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: strong constitutive promoter + luc
creator: Jonas Aretz
created: 2010-10-11T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a firefly luciferase gene under the control of a strong constitutive promoter.
mutable notes: strong promoter, strong RBS, used this to demonstrate that our luciferase BioBrick is working
mutable provenance: * promoter and RBS from partsregistry * luciferase from pGL4.10luc2 (Promega)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_J23102
component: BBa_K389004
sequence annotation: BBa_K389004
sequence annotation: BBa_J23102
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K389318_sequence/1

BBa_B0034
type: Component
persistent identity: component2085587
display id: component2085587
version: 1
top level: BBa_K389318
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_J23102
type: Component
persistent identity: component2085585
display id: component2085585
version: 1
top level: BBa_K389318
access: public
definition: https://synbiohub.org/public/igem/BBa_J23102/1

BBa_K389004
type: Component
persistent identity: component2085590
display id: component2085590
version: 1
top level: BBa_K389318
access: public
definition: https://synbiohub.org/public/igem/BBa_K389004/1

BBa_K389004
type: SequenceAnnotation
persistent identity: annotation2085590
display id: annotation2085590
version: 1
top level: BBa_K389318
location: https://synbiohub.org/public/igem/BBa_K389318/annotation2085590/range2085590/1
component: BBa_K389004

https://synbiohub.org/public/igem/BBa_K389318/annotation2085590/range2085590/1
type: Range
persistent identity: range2085590
display id: range2085590
version: 1
direction: direction/reverse
top level: BBa_K389318
start: 62
end: 1717
orientation: inline

BBa_J23102
type: SequenceAnnotation
persistent identity: annotation2085585
display id: annotation2085585
version: 1
top level: BBa_K389318
location: https://synbiohub.org/public/igem/BBa_K389318/annotation2085585/range2085585/1
component: BBa_J23102

https://synbiohub.org/public/igem/BBa_K389318/annotation2085585/range2085585/1
type: Range
persistent identity: range2085585
display id: range2085585
version: 1
direction: direction/reverse
top level: BBa_K389318
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2085587
display id: annotation2085587
version: 1
top level: BBa_K389318
location: https://synbiohub.org/public/igem/BBa_K389318/annotation2085587/range2085587/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K389318/annotation2085587/range2085587/1
type: Range
persistent identity: range2085587
display id: range2085587
version: 1
direction: direction/reverse
top level: BBa_K389318
start: 44
end: 55
orientation: inline

BBa_I15041
type: ComponentDefinition
persistent identity: BBa I15041
display id: BBa_I15041
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TetR generator
creator: Jeff Tabor
created: 2005-06-01T11:00:00Z
modified: 2015-08-31T04:07:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _5_
m user id: 0
owner id: 88
owning group id: 37
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part uses promoter R0051, a strong promoter which is constitutive in the absence of the lambda cI repressor protein, to drive expression of the tetracycline repressor from the paskiba3 plasmid.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_I15040
component: BBa_R0051
sequence annotation: BBa_R0051
sequence annotation: BBa_I15040
sequence: https://synbiohub.org/public/igem/BBa_I15041_sequence/1

BBa_I15040
type: Component
persistent identity: component1501178
display id: component1501178
version: 1
top level: BBa_I15041
access: public
definition: RBS.TetR

BBa_R0051
type: Component
persistent identity: component1501167
display id: component1501167
version: 1
top level: BBa_I15041
access: public
definition: https://synbiohub.org/public/igem/BBa_R0051/1

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation1501167
display id: annotation1501167
version: 1
top level: BBa_I15041
location: https://synbiohub.org/public/igem/BBa_I15041/annotation1501167/range1501167/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_I15041/annotation1501167/range1501167/1
type: Range
persistent identity: range1501167
display id: range1501167
version: 1
direction: direction/forward
top level: BBa_I15041
start: 1
end: 49
orientation: inline

BBa_I15040
type: SequenceAnnotation
persistent identity: annotation1501178
display id: annotation1501178
version: 1
top level: BBa_I15041
location: https://synbiohub.org/public/igem/BBa_I15041/annotation1501178/range1501178/1
component: BBa_I15040

https://synbiohub.org/public/igem/BBa_I15041/annotation1501178/range1501178/1
type: Range
persistent identity: range1501178
display id: range1501178
version: 1
direction: direction/forward
top level: BBa_I15041
start: 58
end: 699
orientation: inline

BBa_K325209
type: ComponentDefinition
persistent identity: BBa K325209
display id: BBa_K325209
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Green Firefly Luciferase and LRE (under pBAD) L. Cruciata (E. coli optimised)
creator: Anja Hohmann and Will Handley
created: 2010-09-16T11:00:00Z
modified: 2015-05-08T01:12:02Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _446_
m user id: 0
owner id: 7466
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the lux operon from V. fischeri expressed under a pBAD promoter. It emits light when induced by arabinose. luxCDE create a substrate which the luciferase complex of luxA and luxB uses to emit light at a blue wavelength.
mutable notes: Removal of existing lux promoter, and replacement with pBAD
mutable provenance: From pHK555 provided by James Slock ( http://departments.kings.edu/biology/lux/bacterial.html )
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: Pbad
sequence annotation: PC
sequence annotation: BamH1 site
sequence annotation: AraC
sequence annotation: Luciferin Regenerating Enzyme (L. cruciata)
sequence annotation: rbs
sequence annotation: Wild Type Luciferase (L. cruciata)
sequence annotation: I0500
sequence annotation: double stop codon
sequence: https://synbiohub.org/public/igem/BBa_K325209_sequence/1

Pbad
type: SequenceAnnotation
persistent identity: annotation2103103
display id: annotation2103103
version: 1
top level: BBa_K325209
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K325209/annotation2103103/range2103103/1

https://synbiohub.org/public/igem/BBa_K325209/annotation2103103/range2103103/1
type: Range
persistent identity: range2103103
display id: range2103103
version: 1
direction: direction/forward
top level: BBa_K325209
start: 1080
end: 1210
orientation: inline

PC
type: SequenceAnnotation
persistent identity: annotation2103102
display id: annotation2103102
version: 1
top level: BBa_K325209
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K325209/annotation2103102/range2103102/1

https://synbiohub.org/public/igem/BBa_K325209/annotation2103102/range2103102/1
type: Range
persistent identity: range2103102
display id: range2103102
version: 1
direction: direction/forward
top level: BBa_K325209
start: 1043
end: 1114
orientation: inline

BamH1 site
type: SequenceAnnotation
persistent identity: annotation2103107
display id: annotation2103107
version: 1
top level: BBa_K325209
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K325209/annotation2103107/range2103107/1

https://synbiohub.org/public/igem/BBa_K325209/annotation2103107/range2103107/1
type: Range
persistent identity: range2103107
display id: range2103107
version: 1
direction: direction/forward
top level: BBa_K325209
start: 2169
end: 2174
orientation: inline

AraC
type: SequenceAnnotation
persistent identity: annotation2103101
display id: annotation2103101
version: 1
top level: BBa_K325209
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K325209/annotation2103101/range2103101/1

https://synbiohub.org/public/igem/BBa_K325209/annotation2103101/range2103101/1
type: Range
persistent identity: range2103101
display id: range2103101
version: 1
direction: direction/forward
top level: BBa_K325209
start: 1
end: 1079
orientation: inline

Luciferin Regenerating Enzyme (L. cruciata)
type: SequenceAnnotation
persistent identity: annotation2103105
display id: annotation2103105
version: 1
top level: BBa_K325209
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K325209/annotation2103105/range2103105/1

https://synbiohub.org/public/igem/BBa_K325209/annotation2103105/range2103105/1
type: Range
persistent identity: range2103105
display id: range2103105
version: 1
direction: direction/forward
top level: BBa_K325209
start: 1236
end: 2162
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2103108
display id: annotation2103108
version: 1
top level: BBa_K325209
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K325209/annotation2103108/range2103108/1

https://synbiohub.org/public/igem/BBa_K325209/annotation2103108/range2103108/1
type: Range
persistent identity: range2103108
display id: range2103108
version: 1
direction: direction/forward
top level: BBa_K325209
start: 2175
end: 2191
orientation: inline

Wild Type Luciferase (L. cruciata)
type: SequenceAnnotation
persistent identity: annotation2103109
display id: annotation2103109
version: 1
top level: BBa_K325209
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K325209/annotation2103109/range2103109/1

https://synbiohub.org/public/igem/BBa_K325209/annotation2103109/range2103109/1
type: Range
persistent identity: range2103109
display id: range2103109
version: 1
direction: direction/forward
top level: BBa_K325209
start: 2192
end: 3835
orientation: inline

I0500
type: SequenceAnnotation
persistent identity: annotation2103100
display id: annotation2103100
version: 1
top level: BBa_K325209
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K325209/annotation2103100/range2103100/1

https://synbiohub.org/public/igem/BBa_K325209/annotation2103100/range2103100/1
type: Range
persistent identity: range2103100
display id: range2103100
version: 1
direction: direction/forward
top level: BBa_K325209
start: 1
end: 1210
orientation: inline

double stop codon
type: SequenceAnnotation
persistent identity: annotation2103119
display id: annotation2103119
version: 1
top level: BBa_K325209
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K325209/annotation2103119/range2103119/1

https://synbiohub.org/public/igem/BBa_K325209/annotation2103119/range2103119/1
type: Range
persistent identity: range2103119
display id: range2103119
version: 1
direction: direction/forward
top level: BBa_K325209
start: 3836
end: 3841
orientation: inline

double stop codon
type: SequenceAnnotation
persistent identity: annotation2103106
display id: annotation2103106
version: 1
top level: BBa_K325209
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K325209/annotation2103106/range2103106/1

https://synbiohub.org/public/igem/BBa_K325209/annotation2103106/range2103106/1
type: Range
persistent identity: range2103106
display id: range2103106
version: 1
direction: direction/forward
top level: BBa_K325209
start: 2163
end: 2168
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2103104
display id: annotation2103104
version: 1
top level: BBa_K325209
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K325209/annotation2103104/range2103104/1

https://synbiohub.org/public/igem/BBa_K325209/annotation2103104/range2103104/1
type: Range
persistent identity: range2103104
display id: range2103104
version: 1
direction: direction/forward
top level: BBa_K325209
start: 1219
end: 1235
orientation: inline

BBa_J07082
type: ComponentDefinition
persistent identity: BBa J07082
display id: BBa_J07082
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tetR promoter+rbs+scfv-FecA 155+rbs+FecI+rbs+FecR'+terminator
creator: Annie Vo
created: 2005-09-27T11:00:00Z
modified: 2015-08-31T04:08:20Z
discontinued: false
dominant: false
group u list: _21_
m user id: 0
owner id: 353
owning group id: 21
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part expresses scFv-fused FecA' protein, FecI, and FecR' proteins. Fusion is at aa 155 of FecA' protein. All are under control of tetR promoter.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J07068
component: BBa_J07072
component: BBa_J07048
component: BBa_J13002
sequence annotation: BBa_J07068
sequence annotation: BBa_J13002
sequence annotation: BBa_J07048
sequence annotation: BBa_J07072
sequence: https://synbiohub.org/public/igem/BBa_J07082_sequence/1

BBa_J07068
type: Component
persistent identity: component2227104
display id: component2227104
version: 1
top level: BBa_J07082
access: public
definition: https://synbiohub.org/public/igem/BBa_J07068/1

BBa_J07072
type: Component
persistent identity: component2227116
display id: component2227116
version: 1
top level: BBa_J07082
access: public
definition: https://synbiohub.org/public/igem/BBa_J07072/1

BBa_J07048
type: Component
persistent identity: component2227098
display id: component2227098
version: 1
top level: BBa_J07082
access: public
definition: https://synbiohub.org/public/igem/BBa_J07048/1

BBa_J13002
type: Component
persistent identity: component2227092
display id: component2227092
version: 1
top level: BBa_J07082
access: public
definition: https://synbiohub.org/public/igem/BBa_J13002/1

BBa_J07068
type: SequenceAnnotation
persistent identity: annotation2227104
display id: annotation2227104
version: 1
top level: BBa_J07082
location: https://synbiohub.org/public/igem/BBa_J07082/annotation2227104/range2227104/1
component: BBa_J07068

https://synbiohub.org/public/igem/BBa_J07082/annotation2227104/range2227104/1
type: Range
persistent identity: range2227104
display id: range2227104
version: 1
direction: direction/reverse
top level: BBa_J07082
start: 3236
end: 3784
orientation: inline

BBa_J13002
type: SequenceAnnotation
persistent identity: annotation2227092
display id: annotation2227092
version: 1
top level: BBa_J07082
location: https://synbiohub.org/public/igem/BBa_J07082/annotation2227092/range2227092/1
component: BBa_J13002

https://synbiohub.org/public/igem/BBa_J07082/annotation2227092/range2227092/1
type: Range
persistent identity: range2227092
display id: range2227092
version: 1
direction: direction/reverse
top level: BBa_J07082
start: 1
end: 74
orientation: inline

BBa_J07048
type: SequenceAnnotation
persistent identity: annotation2227098
display id: annotation2227098
version: 1
top level: BBa_J07082
location: https://synbiohub.org/public/igem/BBa_J07082/annotation2227098/range2227098/1
component: BBa_J07048

https://synbiohub.org/public/igem/BBa_J07082/annotation2227098/range2227098/1
type: Range
persistent identity: range2227098
display id: range2227098
version: 1
direction: direction/reverse
top level: BBa_J07082
start: 81
end: 3227
orientation: inline

BBa_J07072
type: SequenceAnnotation
persistent identity: annotation2227116
display id: annotation2227116
version: 1
top level: BBa_J07082
location: https://synbiohub.org/public/igem/BBa_J07082/annotation2227116/range2227116/1
component: BBa_J07072

https://synbiohub.org/public/igem/BBa_J07082/annotation2227116/range2227116/1
type: Range
persistent identity: range2227116
display id: range2227116
version: 1
direction: direction/reverse
top level: BBa_J07082
start: 3793
end: 4904
orientation: inline

BBa_K389307
type: ComponentDefinition
persistent identity: BBa K389307
display id: BBa_K389307
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: medium strong constitutive promoter + luc
creator: Jonas Aretz
created: 2010-10-11T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a firefly luciferase gene under the control of a medium strong constitutive promoter.
mutable notes: medium strong promoter, strong RBS, used this to demonstrate that our luciferase BioBrick is working
mutable provenance: * promoter and RBS from partsregistry * luciferase from pGL4.10luc2 (Promega)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_K389004
component: BBa_J23115
sequence annotation: BBa_J23115
sequence annotation: BBa_B0034
sequence annotation: BBa_K389004
sequence: https://synbiohub.org/public/igem/BBa_K389307_sequence/1

BBa_B0034
type: Component
persistent identity: component2085581
display id: component2085581
version: 1
top level: BBa_K389307
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K389004
type: Component
persistent identity: component2085584
display id: component2085584
version: 1
top level: BBa_K389307
access: public
definition: https://synbiohub.org/public/igem/BBa_K389004/1

BBa_J23115
type: Component
persistent identity: component2085579
display id: component2085579
version: 1
top level: BBa_K389307
access: public
definition: https://synbiohub.org/public/igem/BBa_J23115/1

BBa_J23115
type: SequenceAnnotation
persistent identity: annotation2085579
display id: annotation2085579
version: 1
top level: BBa_K389307
location: https://synbiohub.org/public/igem/BBa_K389307/annotation2085579/range2085579/1
component: BBa_J23115

https://synbiohub.org/public/igem/BBa_K389307/annotation2085579/range2085579/1
type: Range
persistent identity: range2085579
display id: range2085579
version: 1
direction: direction/reverse
top level: BBa_K389307
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2085581
display id: annotation2085581
version: 1
top level: BBa_K389307
location: https://synbiohub.org/public/igem/BBa_K389307/annotation2085581/range2085581/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K389307/annotation2085581/range2085581/1
type: Range
persistent identity: range2085581
display id: range2085581
version: 1
direction: direction/reverse
top level: BBa_K389307
start: 44
end: 55
orientation: inline

BBa_K389004
type: SequenceAnnotation
persistent identity: annotation2085584
display id: annotation2085584
version: 1
top level: BBa_K389307
location: https://synbiohub.org/public/igem/BBa_K389307/annotation2085584/range2085584/1
component: BBa_K389004

https://synbiohub.org/public/igem/BBa_K389307/annotation2085584/range2085584/1
type: Range
persistent identity: range2085584
display id: range2085584
version: 1
direction: direction/reverse
top level: BBa_K389307
start: 62
end: 1717
orientation: inline

BBa_K206002
type: ComponentDefinition
persistent identity: BBa K206002
display id: BBa_K206002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP generator for testing pBAD strong
creator: Amelia Hardjasa
created: 2009-10-17T11:00:00Z
modified: 2015-05-08T01:11:23Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _307_
m user id: 0
owner id: 4172
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: We used this construct to test pBAD strong promoter strength by measuring RFP fluorescence.
mutable notes: No special design considerations.
mutable provenance: Assembled from Registry BioBricks.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_I13507
component: BBa_K206000
sequence annotation: BBa_K206000
sequence annotation: BBa_I13507
sequence: https://synbiohub.org/public/igem/BBa_K206002_sequence/1

BBa_I13507
type: Component
persistent identity: component2256273
display id: component2256273
version: 1
top level: BBa_K206002
access: public
definition: https://synbiohub.org/public/igem/BBa_I13507/1

BBa_K206000
type: Component
persistent identity: component2256261
display id: component2256261
version: 1
top level: BBa_K206002
access: public
definition: https://synbiohub.org/public/igem/BBa_K206000/1

BBa_K206000
type: SequenceAnnotation
persistent identity: annotation2256261
display id: annotation2256261
version: 1
top level: BBa_K206002
location: https://synbiohub.org/public/igem/BBa_K206002/annotation2256261/range2256261/1
component: BBa_K206000

https://synbiohub.org/public/igem/BBa_K206002/annotation2256261/range2256261/1
type: Range
persistent identity: range2256261
display id: range2256261
version: 1
direction: direction/reverse
top level: BBa_K206002
start: 1
end: 130
orientation: inline

BBa_I13507
type: SequenceAnnotation
persistent identity: annotation2256273
display id: annotation2256273
version: 1
top level: BBa_K206002
location: https://synbiohub.org/public/igem/BBa_K206002/annotation2256273/range2256273/1
component: BBa_I13507

https://synbiohub.org/public/igem/BBa_K206002/annotation2256273/range2256273/1
type: Range
persistent identity: range2256273
display id: range2256273
version: 1
direction: direction/reverse
top level: BBa_K206002
start: 139
end: 999
orientation: inline

BBa_K780004
type: ComponentDefinition
persistent identity: BBa K780004
display id: BBa_K780004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS+GFP for expression in Bacillus subtilis
creator: iGEM 2012 Warsaw Team
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1034_
m user id: 0
owner id: 10113
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: It is succesfully joined B.subtilis RBS with GFP reporter protein. Can be used to measure strenght of B.subtilis promoters. In order to work with it you must first add appropriate terminator at the end. We recommend BBa_K780000 - a terminator designed for use with B.subtilis.
mutable notes: -
mutable provenance: RBS was taken from 'host-takover module' from B.subtilis Bacteriophage SPO1 and GPF is derived from jellyfish Aequeora victoria (BBa_E0040).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K780002
component: BBa_E0040
sequence annotation: BBa_K780002
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_K780004_sequence/1

BBa_K780002
type: Component
persistent identity: component2200424
display id: component2200424
version: 1
top level: BBa_K780004
access: public
definition: https://synbiohub.org/public/igem/BBa_K780002/1

BBa_E0040
type: Component
persistent identity: component2200426
display id: component2200426
version: 1
top level: BBa_K780004
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_K780002
type: SequenceAnnotation
persistent identity: annotation2200424
display id: annotation2200424
version: 1
top level: BBa_K780004
location: https://synbiohub.org/public/igem/BBa_K780004/annotation2200424/range2200424/1
component: BBa_K780002

https://synbiohub.org/public/igem/BBa_K780004/annotation2200424/range2200424/1
type: Range
persistent identity: range2200424
display id: range2200424
version: 1
direction: direction/reverse
top level: BBa_K780004
start: 1
end: 16
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2200426
display id: annotation2200426
version: 1
top level: BBa_K780004
location: https://synbiohub.org/public/igem/BBa_K780004/annotation2200426/range2200426/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K780004/annotation2200426/range2200426/1
type: Range
persistent identity: range2200426
display id: range2200426
version: 1
direction: direction/reverse
top level: BBa_K780004
start: 23
end: 742
orientation: inline

virA
type: ComponentDefinition
persistent identity: BBa K389010
display id: BBa_K389010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: VirA receptor (expressed)
creator: Jonas Aretz
created: 2010-08-09T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This BioBrick contains a ''virA'' receptor from ''Agrobacterium tumefaciens'' C58 under the control of a constitutive promotor. We used it to mutate ''virA'' by error-prone PCR. For screening the mutants BBa_K389011 is also needed.
mutable notes: The ''virA'' gene is under the control a medium strong constitutive promotor.
mutable provenance: ''A. tumefaciens'' C58, partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_K389001
component: BBa_J23110
sequence annotation: BBa_K389001
sequence annotation: BBa_J23110
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K389010_sequence/1

BBa_B0034
type: Component
persistent identity: component2219529
display id: component2219529
version: 1
top level: virA
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K389001
type: Component
persistent identity: component2219533
display id: component2219533
version: 1
top level: virA
access: public
definition: https://synbiohub.org/public/igem/BBa_K389001/1

BBa_J23110
type: Component
persistent identity: component2219527
display id: component2219527
version: 1
top level: virA
access: public
definition: https://synbiohub.org/public/igem/BBa_J23110/1

BBa_K389001
type: SequenceAnnotation
persistent identity: annotation2219533
display id: annotation2219533
version: 1
top level: virA
location: https://synbiohub.org/public/igem/BBa_K389010/annotation2219533/range2219533/1
component: BBa_K389001

https://synbiohub.org/public/igem/BBa_K389010/annotation2219533/range2219533/1
type: Range
persistent identity: range2219533
display id: range2219533
version: 1
direction: direction/reverse
top level: virA
start: 62
end: 2566
orientation: inline

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2219527
display id: annotation2219527
version: 1
top level: virA
location: https://synbiohub.org/public/igem/BBa_K389010/annotation2219527/range2219527/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K389010/annotation2219527/range2219527/1
type: Range
persistent identity: range2219527
display id: range2219527
version: 1
direction: direction/reverse
top level: virA
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2219529
display id: annotation2219529
version: 1
top level: virA
location: https://synbiohub.org/public/igem/BBa_K389010/annotation2219529/range2219529/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K389010/annotation2219529/range2219529/1
type: Range
persistent identity: range2219529
display id: range2219529
version: 1
direction: direction/reverse
top level: virA
start: 44
end: 55
orientation: inline

BBa_K325239
type: ComponentDefinition
persistent identity: BBa K325239
display id: BBa_K325239
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Red Firefly Luciferase and LRE (under pBAD) L. Cruciata (E. coli optimised)
creator: Theo Sanderson and Hannah Copley
created: 2010-10-22T11:00:00Z
modified: 2015-05-08T01:12:02Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _446_
m user id: 0
owner id: 7466
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: BBa_I0500
sequence annotation: BamH1 site
sequence annotation: Pbad
sequence annotation: Gly326Ser
sequence annotation: rbs
sequence annotation: AraC
sequence annotation: double stop codon
sequence annotation: PC
sequence annotation: Luciferin Regenerating Enzyme (L. cruciata)
sequence annotation: Red Luciferase (326) (L. cruciata)
sequence: https://synbiohub.org/public/igem/BBa_K325239_sequence/1

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2103781
display id: annotation2103781
version: 1
top level: BBa_K325239
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K325239/annotation2103781/range2103781/1

https://synbiohub.org/public/igem/BBa_K325239/annotation2103781/range2103781/1
type: Range
persistent identity: range2103781
display id: range2103781
version: 1
direction: direction/forward
top level: BBa_K325239
start: 1
end: 1210
orientation: inline

BamH1 site
type: SequenceAnnotation
persistent identity: annotation2103791
display id: annotation2103791
version: 1
top level: BBa_K325239
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K325239/annotation2103791/range2103791/1

https://synbiohub.org/public/igem/BBa_K325239/annotation2103791/range2103791/1
type: Range
persistent identity: range2103791
display id: range2103791
version: 1
direction: direction/forward
top level: BBa_K325239
start: 2169
end: 2174
orientation: inline

Pbad
type: SequenceAnnotation
persistent identity: annotation2103784
display id: annotation2103784
version: 1
top level: BBa_K325239
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K325239/annotation2103784/range2103784/1

https://synbiohub.org/public/igem/BBa_K325239/annotation2103784/range2103784/1
type: Range
persistent identity: range2103784
display id: range2103784
version: 1
direction: direction/forward
top level: BBa_K325239
start: 1080
end: 1210
orientation: inline

Gly326Ser
type: SequenceAnnotation
persistent identity: annotation2103879
display id: annotation2103879
version: 1
top level: BBa_K325239
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K325239/annotation2103879/range2103879/1

https://synbiohub.org/public/igem/BBa_K325239/annotation2103879/range2103879/1
type: Range
persistent identity: range2103879
display id: range2103879
version: 1
direction: direction/forward
top level: BBa_K325239
start: 3167
end: 3167
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2103785
display id: annotation2103785
version: 1
top level: BBa_K325239
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K325239/annotation2103785/range2103785/1

https://synbiohub.org/public/igem/BBa_K325239/annotation2103785/range2103785/1
type: Range
persistent identity: range2103785
display id: range2103785
version: 1
direction: direction/forward
top level: BBa_K325239
start: 1219
end: 1235
orientation: inline

AraC
type: SequenceAnnotation
persistent identity: annotation2103782
display id: annotation2103782
version: 1
top level: BBa_K325239
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K325239/annotation2103782/range2103782/1

https://synbiohub.org/public/igem/BBa_K325239/annotation2103782/range2103782/1
type: Range
persistent identity: range2103782
display id: range2103782
version: 1
direction: direction/forward
top level: BBa_K325239
start: 1
end: 1079
orientation: inline

double stop codon
type: SequenceAnnotation
persistent identity: annotation2103794
display id: annotation2103794
version: 1
top level: BBa_K325239
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K325239/annotation2103794/range2103794/1

https://synbiohub.org/public/igem/BBa_K325239/annotation2103794/range2103794/1
type: Range
persistent identity: range2103794
display id: range2103794
version: 1
direction: direction/forward
top level: BBa_K325239
start: 3836
end: 3841
orientation: inline

PC
type: SequenceAnnotation
persistent identity: annotation2103783
display id: annotation2103783
version: 1
top level: BBa_K325239
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K325239/annotation2103783/range2103783/1

https://synbiohub.org/public/igem/BBa_K325239/annotation2103783/range2103783/1
type: Range
persistent identity: range2103783
display id: range2103783
version: 1
direction: direction/forward
top level: BBa_K325239
start: 1043
end: 1114
orientation: inline

Luciferin Regenerating Enzyme (L. cruciata)
type: SequenceAnnotation
persistent identity: annotation2103786
display id: annotation2103786
version: 1
top level: BBa_K325239
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K325239/annotation2103786/range2103786/1

https://synbiohub.org/public/igem/BBa_K325239/annotation2103786/range2103786/1
type: Range
persistent identity: range2103786
display id: range2103786
version: 1
direction: direction/forward
top level: BBa_K325239
start: 1236
end: 2162
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2103792
display id: annotation2103792
version: 1
top level: BBa_K325239
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K325239/annotation2103792/range2103792/1

https://synbiohub.org/public/igem/BBa_K325239/annotation2103792/range2103792/1
type: Range
persistent identity: range2103792
display id: range2103792
version: 1
direction: direction/forward
top level: BBa_K325239
start: 2175
end: 2191
orientation: inline

double stop codon
type: SequenceAnnotation
persistent identity: annotation2103788
display id: annotation2103788
version: 1
top level: BBa_K325239
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K325239/annotation2103788/range2103788/1

https://synbiohub.org/public/igem/BBa_K325239/annotation2103788/range2103788/1
type: Range
persistent identity: range2103788
display id: range2103788
version: 1
direction: direction/forward
top level: BBa_K325239
start: 2163
end: 2168
orientation: inline

Red Luciferase (326) (L. cruciata)
type: SequenceAnnotation
persistent identity: annotation2103793
display id: annotation2103793
version: 1
top level: BBa_K325239
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K325239/annotation2103793/range2103793/1

https://synbiohub.org/public/igem/BBa_K325239/annotation2103793/range2103793/1
type: Range
persistent identity: range2103793
display id: range2103793
version: 1
direction: direction/forward
top level: BBa_K325239
start: 2192
end: 3835
orientation: inline

BBa_I13971
type: ComponentDefinition
persistent identity: BBa I13971
display id: BBa_I13971
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: c1 CFP Protein Generator (R0051.E0420)
creator: robertb
created: 2004-08-08T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: true
experience: experience/None
group u list: _6_
m user id: 0
owner id: 159
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A CFP protein generator under control of c1 for use as a negative control with the Synchronator constructs.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0010
component: BBa_E0020
component: BBa_B0012
component: BBa_R0051
component: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_E0020
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_R0051
sequence: https://synbiohub.org/public/igem/BBa_I13971_sequence/1

BBa_B0010
type: Component
persistent identity: component1040700
display id: component1040700
version: 1
top level: BBa_I13971
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0020
type: Component
persistent identity: component1040695
display id: component1040695
version: 1
top level: BBa_I13971
access: public
definition: https://synbiohub.org/public/igem/BBa_E0020/1

BBa_B0012
type: Component
persistent identity: component1040710
display id: component1040710
version: 1
top level: BBa_I13971
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0051
type: Component
persistent identity: component1040685
display id: component1040685
version: 1
top level: BBa_I13971
access: public
definition: https://synbiohub.org/public/igem/BBa_R0051/1

BBa_B0034
type: Component
persistent identity: component1040693
display id: component1040693
version: 1
top level: BBa_I13971
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1040710
display id: annotation1040710
version: 1
top level: BBa_I13971
location: https://synbiohub.org/public/igem/BBa_I13971/annotation1040710/range1040710/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13971/annotation1040710/range1040710/1
type: Range
persistent identity: range1040710
display id: range1040710
version: 1
direction: direction/forward
top level: BBa_I13971
start: 895
end: 935
orientation: inline

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation1040695
display id: annotation1040695
version: 1
top level: BBa_I13971
location: https://synbiohub.org/public/igem/BBa_I13971/annotation1040695/range1040695/1
component: BBa_E0020

https://synbiohub.org/public/igem/BBa_I13971/annotation1040695/range1040695/1
type: Range
persistent identity: range1040695
display id: range1040695
version: 1
direction: direction/forward
top level: BBa_I13971
start: 76
end: 798
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1040693
display id: annotation1040693
version: 1
top level: BBa_I13971
location: https://synbiohub.org/public/igem/BBa_I13971/annotation1040693/range1040693/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13971/annotation1040693/range1040693/1
type: Range
persistent identity: range1040693
display id: range1040693
version: 1
direction: direction/forward
top level: BBa_I13971
start: 58
end: 69
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1040700
display id: annotation1040700
version: 1
top level: BBa_I13971
location: https://synbiohub.org/public/igem/BBa_I13971/annotation1040700/range1040700/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13971/annotation1040700/range1040700/1
type: Range
persistent identity: range1040700
display id: range1040700
version: 1
direction: direction/forward
top level: BBa_I13971
start: 807
end: 886
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation1040685
display id: annotation1040685
version: 1
top level: BBa_I13971
location: https://synbiohub.org/public/igem/BBa_I13971/annotation1040685/range1040685/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_I13971/annotation1040685/range1040685/1
type: Range
persistent identity: range1040685
display id: range1040685
version: 1
direction: direction/forward
top level: BBa_I13971
start: 1
end: 49
orientation: inline

BBa_I12023
type: ComponentDefinition
persistent identity: BBa I12023
display id: BBa_I12023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Inducible Lambda cI Repressor Generator (Controlled by ATC and TetR)
creator: ryhsiao
created: 2004-07-25T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 148
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part will produce Lambda cI Repressor when ATC is introduced into the system. The system must also be free of p22 cII for Lambda cI repressor to be expressed.
mutable notes: The absence of p22 cII (constitutive promoter) will yield a constant promotion of TetR, which inhibits the expression of lambda cI. Once ATC is introduced into the system, it will bind to the TetR, resulting in expression/promotion of lambda cI repressor via the QPI (Quad Part Inverter). Hence, a switch controlled by ATC is created.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0012
component: BBa_B0010
component: BBa_R0053
component: BBa_B0034
component: BBa_C0040
component: BBa_R0040
component: BBa_C0051
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_C0040
sequence annotation: BBa_C0051
sequence annotation: BBa_B0034
sequence annotation: BBa_R0053
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_I12023_sequence/1

BBa_B0012
type: Component
persistent identity: component965351
display id: component965351
version: 1
top level: BBa_I12023
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component965390
display id: component965390
version: 1
top level: BBa_I12023
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
type: Component
persistent identity: component965341
display id: component965341
version: 1
top level: BBa_I12023
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0053
type: Component
persistent identity: component965315
display id: component965315
version: 1
top level: BBa_I12023
access: public
definition: https://synbiohub.org/public/igem/BBa_R0053/1

BBa_B0034
type: Component
persistent identity: component965325
display id: component965325
version: 1
top level: BBa_I12023
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component965374
display id: component965374
version: 1
top level: BBa_I12023
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component965400
display id: component965400
version: 1
top level: BBa_I12023
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0040
type: Component
persistent identity: component965335
display id: component965335
version: 1
top level: BBa_I12023
access: public
definition: https://synbiohub.org/public/igem/BBa_C0040/1

BBa_R0040
type: Component
persistent identity: component965366
display id: component965366
version: 1
top level: BBa_I12023
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_C0051
type: Component
persistent identity: component965384
display id: component965384
version: 1
top level: BBa_I12023
access: public
definition: https://synbiohub.org/public/igem/BBa_C0051/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation965400
display id: annotation965400
version: 1
top level: BBa_I12023
location: https://synbiohub.org/public/igem/BBa_I12023/annotation965400/range965400/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I12023/annotation965400/range965400/1
type: Range
persistent identity: range965400
display id: range965400
version: 1
direction: direction/forward
top level: BBa_I12023
start: 1862
end: 1902
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation965390
display id: annotation965390
version: 1
top level: BBa_I12023
location: https://synbiohub.org/public/igem/BBa_I12023/annotation965390/range965390/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I12023/annotation965390/range965390/1
type: Range
persistent identity: range965390
display id: range965390
version: 1
direction: direction/forward
top level: BBa_I12023
start: 1774
end: 1853
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation965335
display id: annotation965335
version: 1
top level: BBa_I12023
location: https://synbiohub.org/public/igem/BBa_I12023/annotation965335/range965335/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_I12023/annotation965335/range965335/1
type: Range
persistent identity: range965335
display id: range965335
version: 1
direction: direction/forward
top level: BBa_I12023
start: 81
end: 740
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation965384
display id: annotation965384
version: 1
top level: BBa_I12023
location: https://synbiohub.org/public/igem/BBa_I12023/annotation965384/range965384/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_I12023/annotation965384/range965384/1
type: Range
persistent identity: range965384
display id: range965384
version: 1
direction: direction/forward
top level: BBa_I12023
start: 991
end: 1740
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation965374
display id: annotation965374
version: 1
top level: BBa_I12023
location: https://synbiohub.org/public/igem/BBa_I12023/annotation965374/range965374/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I12023/annotation965374/range965374/1
type: Range
persistent identity: range965374
display id: range965374
version: 1
direction: direction/forward
top level: BBa_I12023
start: 973
end: 984
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation965351
display id: annotation965351
version: 1
top level: BBa_I12023
location: https://synbiohub.org/public/igem/BBa_I12023/annotation965351/range965351/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I12023/annotation965351/range965351/1
type: Range
persistent identity: range965351
display id: range965351
version: 1
direction: direction/forward
top level: BBa_I12023
start: 862
end: 902
orientation: inline

BBa_R0053
type: SequenceAnnotation
persistent identity: annotation965315
display id: annotation965315
version: 1
top level: BBa_I12023
location: https://synbiohub.org/public/igem/BBa_I12023/annotation965315/range965315/1
component: BBa_R0053

https://synbiohub.org/public/igem/BBa_I12023/annotation965315/range965315/1
type: Range
persistent identity: range965315
display id: range965315
version: 1
direction: direction/forward
top level: BBa_I12023
start: 1
end: 54
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation965366
display id: annotation965366
version: 1
top level: BBa_I12023
location: https://synbiohub.org/public/igem/BBa_I12023/annotation965366/range965366/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I12023/annotation965366/range965366/1
type: Range
persistent identity: range965366
display id: range965366
version: 1
direction: direction/forward
top level: BBa_I12023
start: 911
end: 964
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation965325
display id: annotation965325
version: 1
top level: BBa_I12023
location: https://synbiohub.org/public/igem/BBa_I12023/annotation965325/range965325/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I12023/annotation965325/range965325/1
type: Range
persistent identity: range965325
display id: range965325
version: 1
direction: direction/forward
top level: BBa_I12023
start: 63
end: 74
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation965341
display id: annotation965341
version: 1
top level: BBa_I12023
location: https://synbiohub.org/public/igem/BBa_I12023/annotation965341/range965341/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I12023/annotation965341/range965341/1
type: Range
persistent identity: range965341
display id: range965341
version: 1
direction: direction/forward
top level: BBa_I12023
start: 774
end: 853
orientation: inline

BBa_J13101
type: ComponentDefinition
persistent identity: BBa J13101
display id: BBa_J13101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacI repressed lambda CI generator
creator: Laura Lavery
created: 2005-07-14T11:00:00Z
modified: 2015-08-31T04:08:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _37_
m user id: 0
owner id: 327
owning group id: 37
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Lambda cI (LVA) is expressed under control of the lacI repressible promoter R0011.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0010
component: BBa_R0011
component: BBa_C0051
component: BBa_B0034
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_R0011
sequence annotation: BBa_C0051
sequence: https://synbiohub.org/public/igem/BBa_J13101_sequence/1

BBa_B0010
type: Component
persistent identity: component1582386
display id: component1582386
version: 1
top level: BBa_J13101
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0011
type: Component
persistent identity: component1582361
display id: component1582361
version: 1
top level: BBa_J13101
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_C0051
type: Component
persistent identity: component1582380
display id: component1582380
version: 1
top level: BBa_J13101
access: public
definition: https://synbiohub.org/public/igem/BBa_C0051/1

BBa_B0034
type: Component
persistent identity: component1582370
display id: component1582370
version: 1
top level: BBa_J13101
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component1582396
display id: component1582396
version: 1
top level: BBa_J13101
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1582396
display id: annotation1582396
version: 1
top level: BBa_J13101
location: https://synbiohub.org/public/igem/BBa_J13101/annotation1582396/range1582396/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J13101/annotation1582396/range1582396/1
type: Range
persistent identity: range1582396
display id: range1582396
version: 1
direction: direction/forward
top level: BBa_J13101
start: 953
end: 993
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1582386
display id: annotation1582386
version: 1
top level: BBa_J13101
location: https://synbiohub.org/public/igem/BBa_J13101/annotation1582386/range1582386/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J13101/annotation1582386/range1582386/1
type: Range
persistent identity: range1582386
display id: range1582386
version: 1
direction: direction/forward
top level: BBa_J13101
start: 865
end: 944
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1582370
display id: annotation1582370
version: 1
top level: BBa_J13101
location: https://synbiohub.org/public/igem/BBa_J13101/annotation1582370/range1582370/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J13101/annotation1582370/range1582370/1
type: Range
persistent identity: range1582370
display id: range1582370
version: 1
direction: direction/forward
top level: BBa_J13101
start: 64
end: 75
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1582361
display id: annotation1582361
version: 1
top level: BBa_J13101
location: https://synbiohub.org/public/igem/BBa_J13101/annotation1582361/range1582361/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_J13101/annotation1582361/range1582361/1
type: Range
persistent identity: range1582361
display id: range1582361
version: 1
direction: direction/forward
top level: BBa_J13101
start: 1
end: 54
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation1582380
display id: annotation1582380
version: 1
top level: BBa_J13101
location: https://synbiohub.org/public/igem/BBa_J13101/annotation1582380/range1582380/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_J13101/annotation1582380/range1582380/1
type: Range
persistent identity: range1582380
display id: range1582380
version: 1
direction: direction/forward
top level: BBa_J13101
start: 82
end: 831
orientation: inline

BBa_K389302
type: ComponentDefinition
persistent identity: BBa K389302
display id: BBa_K389302
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: weak constitutive promoter + luc
creator: Jonas Aretz
created: 2010-10-11T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a firefly luciferase gene under the control of a weak constitutive promoter.
mutable notes: weak promoter, strong RBS, used this to demonstrate that our luciferase BioBrick is working
mutable provenance: * promoter and RBS from partsregistry * luciferase from pGL4.10luc2 (Promega)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23103
component: BBa_K389004
component: BBa_B0034
sequence annotation: BBa_J23103
sequence annotation: BBa_K389004
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K389302_sequence/1

BBa_J23103
type: Component
persistent identity: component2085573
display id: component2085573
version: 1
top level: BBa_K389302
access: public
definition: https://synbiohub.org/public/igem/BBa_J23103/1

BBa_K389004
type: Component
persistent identity: component2085578
display id: component2085578
version: 1
top level: BBa_K389302
access: public
definition: https://synbiohub.org/public/igem/BBa_K389004/1

BBa_B0034
type: Component
persistent identity: component2085575
display id: component2085575
version: 1
top level: BBa_K389302
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_J23103
type: SequenceAnnotation
persistent identity: annotation2085573
display id: annotation2085573
version: 1
top level: BBa_K389302
location: https://synbiohub.org/public/igem/BBa_K389302/annotation2085573/range2085573/1
component: BBa_J23103

https://synbiohub.org/public/igem/BBa_K389302/annotation2085573/range2085573/1
type: Range
persistent identity: range2085573
display id: range2085573
version: 1
direction: direction/reverse
top level: BBa_K389302
start: 1
end: 35
orientation: inline

BBa_K389004
type: SequenceAnnotation
persistent identity: annotation2085578
display id: annotation2085578
version: 1
top level: BBa_K389302
location: https://synbiohub.org/public/igem/BBa_K389302/annotation2085578/range2085578/1
component: BBa_K389004

https://synbiohub.org/public/igem/BBa_K389302/annotation2085578/range2085578/1
type: Range
persistent identity: range2085578
display id: range2085578
version: 1
direction: direction/reverse
top level: BBa_K389302
start: 62
end: 1717
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2085575
display id: annotation2085575
version: 1
top level: BBa_K389302
location: https://synbiohub.org/public/igem/BBa_K389302/annotation2085575/range2085575/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K389302/annotation2085575/range2085575/1
type: Range
persistent identity: range2085575
display id: range2085575
version: 1
direction: direction/reverse
top level: BBa_K389302
start: 44
end: 55
orientation: inline

BBa_K1480005
type: ComponentDefinition
persistent identity: BBa K1480005
display id: BBa_K1480005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TetR generator (Promoter J23111 )
creator: Jesus Gilberto Rodriguez Ceja
created: 2014-10-13T11:00:00Z
modified: 2015-05-08T01:10:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1860_
m user id: 0
owner id: 12878
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a TetR protein generator regulated by the constitutive promoter J23111. The protein has a LVA tag for degradation.
mutable notes: It was assembled using assembly 10.
mutable provenance: Based on parts of the registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23111
component: BBa_B0010
component: BBa_B0034
component: BBa_C0040
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_J23111
sequence annotation: BBa_B0034
sequence annotation: BBa_C0040
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_K1480005_sequence/1

BBa_J23111
type: Component
persistent identity: component2419554
display id: component2419554
version: 1
top level: BBa_K1480005
access: public
definition: https://synbiohub.org/public/igem/BBa_J23111/1

BBa_B0010
type: Component
persistent identity: component2419561
display id: component2419561
version: 1
top level: BBa_K1480005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component2419556
display id: component2419556
version: 1
top level: BBa_K1480005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0040
type: Component
persistent identity: component2419560
display id: component2419560
version: 1
top level: BBa_K1480005
access: public
definition: https://synbiohub.org/public/igem/BBa_C0040/1

BBa_B0012
type: Component
persistent identity: component2419563
display id: component2419563
version: 1
top level: BBa_K1480005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2419563
display id: annotation2419563
version: 1
top level: BBa_K1480005
location: https://synbiohub.org/public/igem/BBa_K1480005/annotation2419563/range2419563/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K1480005/annotation2419563/range2419563/1
type: Range
persistent identity: range2419563
display id: range2419563
version: 1
direction: direction/forward
top level: BBa_K1480005
start: 843
end: 883
orientation: inline

BBa_J23111
type: SequenceAnnotation
persistent identity: annotation2419554
display id: annotation2419554
version: 1
top level: BBa_K1480005
location: https://synbiohub.org/public/igem/BBa_K1480005/annotation2419554/range2419554/1
component: BBa_J23111

https://synbiohub.org/public/igem/BBa_K1480005/annotation2419554/range2419554/1
type: Range
persistent identity: range2419554
display id: range2419554
version: 1
direction: direction/reverse
top level: BBa_K1480005
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2419556
display id: annotation2419556
version: 1
top level: BBa_K1480005
location: https://synbiohub.org/public/igem/BBa_K1480005/annotation2419556/range2419556/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1480005/annotation2419556/range2419556/1
type: Range
persistent identity: range2419556
display id: range2419556
version: 1
direction: direction/reverse
top level: BBa_K1480005
start: 44
end: 55
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2419560
display id: annotation2419560
version: 1
top level: BBa_K1480005
location: https://synbiohub.org/public/igem/BBa_K1480005/annotation2419560/range2419560/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K1480005/annotation2419560/range2419560/1
type: Range
persistent identity: range2419560
display id: range2419560
version: 1
direction: direction/reverse
top level: BBa_K1480005
start: 62
end: 746
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2419561
display id: annotation2419561
version: 1
top level: BBa_K1480005
location: https://synbiohub.org/public/igem/BBa_K1480005/annotation2419561/range2419561/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K1480005/annotation2419561/range2419561/1
type: Range
persistent identity: range2419561
display id: range2419561
version: 1
direction: direction/forward
top level: BBa_K1480005
start: 755
end: 834
orientation: inline

BBa_J07084
type: ComponentDefinition
persistent identity: BBa J07084
display id: BBa_J07084
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tetR promoter+rbs+scfv-FecA 176+rbs+FecI+rbs+FecR'+terminator
creator: Annie Vo
created: 2005-09-27T11:00:00Z
modified: 2015-08-31T04:08:20Z
discontinued: false
dominant: false
group u list: _21_
m user id: 0
owner id: 353
owning group id: 21
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part expresses scFv-fused FecA' protein, FecI, and FecR' proteins. Fusion is at aa 176 of FecA' protein. All are under control of tetR promoter.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J13002
component: BBa_J07068
component: BBa_J07072
component: BBa_J07049
sequence annotation: BBa_J07049
sequence annotation: BBa_J07072
sequence annotation: BBa_J13002
sequence annotation: BBa_J07068
sequence: https://synbiohub.org/public/igem/BBa_J07084_sequence/1

BBa_J13002
type: Component
persistent identity: component2227151
display id: component2227151
version: 1
top level: BBa_J07084
access: public
definition: https://synbiohub.org/public/igem/BBa_J13002/1

BBa_J07068
type: Component
persistent identity: component2227163
display id: component2227163
version: 1
top level: BBa_J07084
access: public
definition: https://synbiohub.org/public/igem/BBa_J07068/1

BBa_J07072
type: Component
persistent identity: component2227175
display id: component2227175
version: 1
top level: BBa_J07084
access: public
definition: https://synbiohub.org/public/igem/BBa_J07072/1

BBa_J07049
type: Component
persistent identity: component2227157
display id: component2227157
version: 1
top level: BBa_J07084
access: public
definition: https://synbiohub.org/public/igem/BBa_J07049/1

BBa_J07049
type: SequenceAnnotation
persistent identity: annotation2227157
display id: annotation2227157
version: 1
top level: BBa_J07084
location: https://synbiohub.org/public/igem/BBa_J07084/annotation2227157/range2227157/1
component: BBa_J07049

https://synbiohub.org/public/igem/BBa_J07084/annotation2227157/range2227157/1
type: Range
persistent identity: range2227157
display id: range2227157
version: 1
direction: direction/reverse
top level: BBa_J07084
start: 81
end: 3227
orientation: inline

BBa_J07072
type: SequenceAnnotation
persistent identity: annotation2227175
display id: annotation2227175
version: 1
top level: BBa_J07084
location: https://synbiohub.org/public/igem/BBa_J07084/annotation2227175/range2227175/1
component: BBa_J07072

https://synbiohub.org/public/igem/BBa_J07084/annotation2227175/range2227175/1
type: Range
persistent identity: range2227175
display id: range2227175
version: 1
direction: direction/reverse
top level: BBa_J07084
start: 3793
end: 4904
orientation: inline

BBa_J13002
type: SequenceAnnotation
persistent identity: annotation2227151
display id: annotation2227151
version: 1
top level: BBa_J07084
location: https://synbiohub.org/public/igem/BBa_J07084/annotation2227151/range2227151/1
component: BBa_J13002

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type: Range
persistent identity: range2227151
display id: range2227151
version: 1
direction: direction/reverse
top level: BBa_J07084
start: 1
end: 74
orientation: inline

BBa_J07068
type: SequenceAnnotation
persistent identity: annotation2227163
display id: annotation2227163
version: 1
top level: BBa_J07084
location: https://synbiohub.org/public/igem/BBa_J07084/annotation2227163/range2227163/1
component: BBa_J07068

https://synbiohub.org/public/igem/BBa_J07084/annotation2227163/range2227163/1
type: Range
persistent identity: range2227163
display id: range2227163
version: 1
direction: direction/reverse
top level: BBa_J07084
start: 3236
end: 3784
orientation: inline

BBa_J07032
type: ComponentDefinition
persistent identity: BBa J07032
display id: BBa_J07032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FecI & FecR' test construct
creator: Annie
created: 2005-07-18T11:00:00Z
modified: 2015-08-31T04:08:19Z
discontinued: false
dominant: false
experience: experience/None
group u list: _21_
m user id: 0
owner id: 353
owning group id: 21
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part contains a promoter regulated by LacI, an RBS, coding sequences of FecI and FecR, and terminator.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J07071
component: BBa_E0840
component: BBa_J07067
sequence annotation: BBa_E0840
sequence annotation: BBa_J07067
sequence annotation: BBa_J07071
sequence: https://synbiohub.org/public/igem/BBa_J07032_sequence/1

BBa_J07071
type: Component
persistent identity: component2227046
display id: component2227046
version: 1
top level: BBa_J07032
access: public
definition: https://synbiohub.org/public/igem/BBa_J07071/1

BBa_E0840
type: Component
persistent identity: component2227057
display id: component2227057
version: 1
top level: BBa_J07032
access: public
definition: https://synbiohub.org/public/igem/BBa_E0840/1

BBa_J07067
type: Component
persistent identity: component2227040
display id: component2227040
version: 1
top level: BBa_J07032
access: public
definition: https://synbiohub.org/public/igem/BBa_J07067/1

BBa_E0840
type: SequenceAnnotation
persistent identity: annotation2227057
display id: annotation2227057
version: 1
top level: BBa_J07032
location: https://synbiohub.org/public/igem/BBa_J07032/annotation2227057/range2227057/1
component: BBa_E0840

https://synbiohub.org/public/igem/BBa_J07032/annotation2227057/range2227057/1
type: Range
persistent identity: range2227057
display id: range2227057
version: 1
direction: direction/reverse
top level: BBa_J07032
start: 1749
end: 2626
orientation: inline

BBa_J07067
type: SequenceAnnotation
persistent identity: annotation2227040
display id: annotation2227040
version: 1
top level: BBa_J07032
location: https://synbiohub.org/public/igem/BBa_J07032/annotation2227040/range2227040/1
component: BBa_J07067

https://synbiohub.org/public/igem/BBa_J07032/annotation2227040/range2227040/1
type: Range
persistent identity: range2227040
display id: range2227040
version: 1
direction: direction/reverse
top level: BBa_J07032
start: 1
end: 757
orientation: inline

BBa_J07071
type: SequenceAnnotation
persistent identity: annotation2227046
display id: annotation2227046
version: 1
top level: BBa_J07032
location: https://synbiohub.org/public/igem/BBa_J07032/annotation2227046/range2227046/1
component: BBa_J07071

https://synbiohub.org/public/igem/BBa_J07032/annotation2227046/range2227046/1
type: Range
persistent identity: range2227046
display id: range2227046
version: 1
direction: direction/reverse
top level: BBa_J07032
start: 766
end: 1740
orientation: inline

BBa_J13102
type: ComponentDefinition
persistent identity: BBa J13102
display id: BBa_J13102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pOmpR dependent cI generator
creator: Jeff Tabor
created: 2005-07-17T11:00:00Z
modified: 2015-08-31T04:08:30Z
discontinued: false
dominant: true
experience: experience/None
group u list: _37_
m user id: 0
owner id: 88
owning group id: 37
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Expression of lambda cI (LVA) is driven by the OmpC promoter. The OmpC promoter is active when the transcription factor OmpR is phosphorylated. If using Cph8 (I15010) as the OmpR regulator, this device is active in the dark. This device functions as a partial repressor device for I15030 and J13030
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_R0082
component: BBa_B0010
component: BBa_B0012
component: BBa_C0051
sequence annotation: BBa_B0034
sequence annotation: BBa_C0051
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0082
sequence: https://synbiohub.org/public/igem/BBa_J13102_sequence/1

BBa_B0034
type: Component
persistent identity: component1584224
display id: component1584224
version: 1
top level: BBa_J13102
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0082
type: Component
persistent identity: component1584219
display id: component1584219
version: 1
top level: BBa_J13102
access: public
definition: https://synbiohub.org/public/igem/BBa_R0082/1

BBa_B0010
type: Component
persistent identity: component1584240
display id: component1584240
version: 1
top level: BBa_J13102
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1584250
display id: component1584250
version: 1
top level: BBa_J13102
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0051
type: Component
persistent identity: component1584234
display id: component1584234
version: 1
top level: BBa_J13102
access: public
definition: https://synbiohub.org/public/igem/BBa_C0051/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1584224
display id: annotation1584224
version: 1
top level: BBa_J13102
location: https://synbiohub.org/public/igem/BBa_J13102/annotation1584224/range1584224/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J13102/annotation1584224/range1584224/1
type: Range
persistent identity: range1584224
display id: range1584224
version: 1
direction: direction/forward
top level: BBa_J13102
start: 117
end: 128
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation1584234
display id: annotation1584234
version: 1
top level: BBa_J13102
location: https://synbiohub.org/public/igem/BBa_J13102/annotation1584234/range1584234/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_J13102/annotation1584234/range1584234/1
type: Range
persistent identity: range1584234
display id: range1584234
version: 1
direction: direction/forward
top level: BBa_J13102
start: 135
end: 884
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1584240
display id: annotation1584240
version: 1
top level: BBa_J13102
location: https://synbiohub.org/public/igem/BBa_J13102/annotation1584240/range1584240/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J13102/annotation1584240/range1584240/1
type: Range
persistent identity: range1584240
display id: range1584240
version: 1
direction: direction/forward
top level: BBa_J13102
start: 918
end: 997
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1584250
display id: annotation1584250
version: 1
top level: BBa_J13102
location: https://synbiohub.org/public/igem/BBa_J13102/annotation1584250/range1584250/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J13102/annotation1584250/range1584250/1
type: Range
persistent identity: range1584250
display id: range1584250
version: 1
direction: direction/forward
top level: BBa_J13102
start: 1006
end: 1046
orientation: inline

BBa_R0082
type: SequenceAnnotation
persistent identity: annotation1584219
display id: annotation1584219
version: 1
top level: BBa_J13102
location: https://synbiohub.org/public/igem/BBa_J13102/annotation1584219/range1584219/1
component: BBa_R0082

https://synbiohub.org/public/igem/BBa_J13102/annotation1584219/range1584219/1
type: Range
persistent identity: range1584219
display id: range1584219
version: 1
direction: direction/forward
top level: BBa_J13102
start: 1
end: 108
orientation: inline

BBa_M30003
type: ComponentDefinition
persistent identity: BBa M30003
display id: BBa_M30003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: -- No description --
creator: natalie kuldell
created: 2005-11-24T12:00:00Z
modified: 2015-05-08T01:13:59Z
discontinued: false
dominant: true
group u list: _45_1_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: lambda cI inverter (Q04510) directing transcription of mRFP (I13507). Constructed with standard assembly except PCR from VF and VR primers was used to generate Q04510 fragment with four-part inverter. Cells carrying M30003 are pink, however because PCR was used for cloning there may be silent mutations in the insert. M30003 is clone #1. A second clone will be saved as M30004.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0012
component: BBa_B0010
component: BBa_B0034
component: BBa_R0051
component: BBa_C0051
component: BBa_E1010
sequence annotation: BBa_C0051
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_E1010
sequence annotation: BBa_R0051
sequence: https://synbiohub.org/public/igem/BBa_M30003_sequence/1

BBa_B0012
type: Component
persistent identity: component1751336
display id: component1751336
version: 1
top level: BBa_M30003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1751280
display id: component1751280
version: 1
top level: BBa_M30003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1751264
display id: component1751264
version: 1
top level: BBa_M30003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component1751313
display id: component1751313
version: 1
top level: BBa_M30003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0051
type: Component
persistent identity: component1751305
display id: component1751305
version: 1
top level: BBa_M30003
access: public
definition: https://synbiohub.org/public/igem/BBa_R0051/1

BBa_B0010
type: Component
persistent identity: component1751326
display id: component1751326
version: 1
top level: BBa_M30003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_C0051
type: Component
persistent identity: component1751274
display id: component1751274
version: 1
top level: BBa_M30003
access: public
definition: https://synbiohub.org/public/igem/BBa_C0051/1

BBa_E1010
type: Component
persistent identity: component1751320
display id: component1751320
version: 1
top level: BBa_M30003
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_B0012
type: Component
persistent identity: component1751290
display id: component1751290
version: 1
top level: BBa_M30003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation1751274
display id: annotation1751274
version: 1
top level: BBa_M30003
location: https://synbiohub.org/public/igem/BBa_M30003/annotation1751274/range1751274/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_M30003/annotation1751274/range1751274/1
type: Range
persistent identity: range1751274
display id: range1751274
version: 1
direction: direction/forward
top level: BBa_M30003
start: 19
end: 768
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1751290
display id: annotation1751290
version: 1
top level: BBa_M30003
location: https://synbiohub.org/public/igem/BBa_M30003/annotation1751290/range1751290/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_M30003/annotation1751290/range1751290/1
type: Range
persistent identity: range1751290
display id: range1751290
version: 1
direction: direction/forward
top level: BBa_M30003
start: 890
end: 930
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1751313
display id: annotation1751313
version: 1
top level: BBa_M30003
location: https://synbiohub.org/public/igem/BBa_M30003/annotation1751313/range1751313/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_M30003/annotation1751313/range1751313/1
type: Range
persistent identity: range1751313
display id: range1751313
version: 1
direction: direction/forward
top level: BBa_M30003
start: 996
end: 1007
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1751336
display id: annotation1751336
version: 1
top level: BBa_M30003
location: https://synbiohub.org/public/igem/BBa_M30003/annotation1751336/range1751336/1
component: BBa_B0012

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type: Range
persistent identity: range1751336
display id: range1751336
version: 1
direction: direction/forward
top level: BBa_M30003
start: 1816
end: 1856
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1751280
display id: annotation1751280
version: 1
top level: BBa_M30003
location: https://synbiohub.org/public/igem/BBa_M30003/annotation1751280/range1751280/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_M30003/annotation1751280/range1751280/1
type: Range
persistent identity: range1751280
display id: range1751280
version: 1
direction: direction/forward
top level: BBa_M30003
start: 802
end: 881
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1751320
display id: annotation1751320
version: 1
top level: BBa_M30003
location: https://synbiohub.org/public/igem/BBa_M30003/annotation1751320/range1751320/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_M30003/annotation1751320/range1751320/1
type: Range
persistent identity: range1751320
display id: range1751320
version: 1
direction: direction/forward
top level: BBa_M30003
start: 1014
end: 1694
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1751326
display id: annotation1751326
version: 1
top level: BBa_M30003
location: https://synbiohub.org/public/igem/BBa_M30003/annotation1751326/range1751326/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_M30003/annotation1751326/range1751326/1
type: Range
persistent identity: range1751326
display id: range1751326
version: 1
direction: direction/forward
top level: BBa_M30003
start: 1728
end: 1807
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1751264
display id: annotation1751264
version: 1
top level: BBa_M30003
location: https://synbiohub.org/public/igem/BBa_M30003/annotation1751264/range1751264/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_M30003/annotation1751264/range1751264/1
type: Range
persistent identity: range1751264
display id: range1751264
version: 1
direction: direction/forward
top level: BBa_M30003
start: 1
end: 12
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation1751305
display id: annotation1751305
version: 1
top level: BBa_M30003
location: https://synbiohub.org/public/igem/BBa_M30003/annotation1751305/range1751305/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_M30003/annotation1751305/range1751305/1
type: Range
persistent identity: range1751305
display id: range1751305
version: 1
direction: direction/forward
top level: BBa_M30003
start: 939
end: 987
orientation: inline

BBa_I13972
type: ComponentDefinition
persistent identity: BBa I13972
display id: BBa_I13972
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lux CFP Protein Generator (R0062.E0420)
creator: robertb
created: 2004-08-08T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: true
experience: experience/None
group u list: _6_
m user id: 0
owner id: 159
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A CFP protein generator under control of LuxR for use as a negative control with the Synchronator constructs.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_E0020
component: BBa_B0012
component: BBa_B0010
component: BBa_R0062
sequence annotation: BBa_R0062
sequence annotation: BBa_E0020
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I13972_sequence/1

BBa_B0034
type: Component
persistent identity: component1040731
display id: component1040731
version: 1
top level: BBa_I13972
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0020
type: Component
persistent identity: component1040733
display id: component1040733
version: 1
top level: BBa_I13972
access: public
definition: https://synbiohub.org/public/igem/BBa_E0020/1

BBa_B0012
type: Component
persistent identity: component1040748
display id: component1040748
version: 1
top level: BBa_I13972
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1040738
display id: component1040738
version: 1
top level: BBa_I13972
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0062
type: Component
persistent identity: component1040723
display id: component1040723
version: 1
top level: BBa_I13972
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation1040723
display id: annotation1040723
version: 1
top level: BBa_I13972
location: https://synbiohub.org/public/igem/BBa_I13972/annotation1040723/range1040723/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_I13972/annotation1040723/range1040723/1
type: Range
persistent identity: range1040723
display id: range1040723
version: 1
direction: direction/forward
top level: BBa_I13972
start: 1
end: 55
orientation: inline

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation1040733
display id: annotation1040733
version: 1
top level: BBa_I13972
location: https://synbiohub.org/public/igem/BBa_I13972/annotation1040733/range1040733/1
component: BBa_E0020

https://synbiohub.org/public/igem/BBa_I13972/annotation1040733/range1040733/1
type: Range
persistent identity: range1040733
display id: range1040733
version: 1
direction: direction/forward
top level: BBa_I13972
start: 82
end: 804
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1040738
display id: annotation1040738
version: 1
top level: BBa_I13972
location: https://synbiohub.org/public/igem/BBa_I13972/annotation1040738/range1040738/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13972/annotation1040738/range1040738/1
type: Range
persistent identity: range1040738
display id: range1040738
version: 1
direction: direction/forward
top level: BBa_I13972
start: 813
end: 892
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1040731
display id: annotation1040731
version: 1
top level: BBa_I13972
location: https://synbiohub.org/public/igem/BBa_I13972/annotation1040731/range1040731/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13972/annotation1040731/range1040731/1
type: Range
persistent identity: range1040731
display id: range1040731
version: 1
direction: direction/forward
top level: BBa_I13972
start: 64
end: 75
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1040748
display id: annotation1040748
version: 1
top level: BBa_I13972
location: https://synbiohub.org/public/igem/BBa_I13972/annotation1040748/range1040748/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13972/annotation1040748/range1040748/1
type: Range
persistent identity: range1040748
display id: range1040748
version: 1
direction: direction/forward
top level: BBa_I13972
start: 901
end: 941
orientation: inline

B-carotene
type: ComponentDefinition
persistent identity: BBa K343006
display id: BBa_K343006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Expresses B-carotene monooxygenase on a constitutive promotor
creator: Christian Kurtzhals
created: 2010-10-13T11:00:00Z
modified: 2015-05-08T01:12:08Z
discontinued: false
dominant: false
experience: experience/None
group u list: _465_
m user id: 0
owner id: 6083
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This generator expresses a beta-carotene 15,15'-monooxygenase,on a constitutive promotor, that catalyses retinal production from beta-carotene via the following reaction: beta-carotene + O2 = 2 retinal
mutable notes: The brick requires B-carotene supply to function. This can either be supplied externally, or alternatively the BBa_K274210 B-carotene generator will induce full B-carotene synthesis in E. coli.
mutable provenance: The ninaB gene was originally cloned from drosophila melanogaster, and cDNA was aquired for production of the biobrick from the Drosophila Genome Ressource Center. Promotor+rbs brick BBa_J13002 and terminator BBa_B0015 were used.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_R0040
component: BBa_K343001
component: BBa_B0010
component: BBa_B0012
component: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_K343001
sequence annotation: BBa_B0034
sequence annotation: BBa_R0040
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K343006_sequence/1

BBa_R0040
type: Component
persistent identity: component2085978
display id: component2085978
version: 1
top level: B-carotene
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_K343001
type: Component
persistent identity: component2085988
display id: component2085988
version: 1
top level: B-carotene
access: public
definition: https://synbiohub.org/public/igem/BBa_K343001/1

BBa_B0010
type: Component
persistent identity: component2085989
display id: component2085989
version: 1
top level: B-carotene
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component2085991
display id: component2085991
version: 1
top level: B-carotene
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component2085984
display id: component2085984
version: 1
top level: B-carotene
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2085989
display id: annotation2085989
version: 1
top level: B-carotene
location: https://synbiohub.org/public/igem/BBa_K343006/annotation2085989/range2085989/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K343006/annotation2085989/range2085989/1
type: Range
persistent identity: range2085989
display id: range2085989
version: 1
direction: direction/forward
top level: B-carotene
start: 1952
end: 2031
orientation: inline

BBa_K343001
type: SequenceAnnotation
persistent identity: annotation2085988
display id: annotation2085988
version: 1
top level: B-carotene
location: https://synbiohub.org/public/igem/BBa_K343006/annotation2085988/range2085988/1
component: BBa_K343001

https://synbiohub.org/public/igem/BBa_K343006/annotation2085988/range2085988/1
type: Range
persistent identity: range2085988
display id: range2085988
version: 1
direction: direction/reverse
top level: B-carotene
start: 81
end: 1943
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2085984
display id: annotation2085984
version: 1
top level: B-carotene
location: https://synbiohub.org/public/igem/BBa_K343006/annotation2085984/range2085984/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K343006/annotation2085984/range2085984/1
type: Range
persistent identity: range2085984
display id: range2085984
version: 1
direction: direction/reverse
top level: B-carotene
start: 63
end: 74
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2085978
display id: annotation2085978
version: 1
top level: B-carotene
location: https://synbiohub.org/public/igem/BBa_K343006/annotation2085978/range2085978/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K343006/annotation2085978/range2085978/1
type: Range
persistent identity: range2085978
display id: range2085978
version: 1
direction: direction/forward
top level: B-carotene
start: 1
end: 54
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2085991
display id: annotation2085991
version: 1
top level: B-carotene
location: https://synbiohub.org/public/igem/BBa_K343006/annotation2085991/range2085991/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K343006/annotation2085991/range2085991/1
type: Range
persistent identity: range2085991
display id: range2085991
version: 1
direction: direction/forward
top level: B-carotene
start: 2040
end: 2080
orientation: inline

BBa_I15023
type: ComponentDefinition
persistent identity: BBa I15023
display id: BBa_I15023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: monocistronic CFP+YFP generator
creator: Jeff Tabor
created: 2005-06-13T11:00:00Z
modified: 2015-08-31T04:07:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _5_
m user id: 0
owner id: 88
owning group id: 37
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part generates ~98.5% monocistronic CFP and YFP + ~1.5% polycistronic CFP/YFP based on the termination efficiency of B0015.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0010
component: BBa_B0034
component: BBa_E0030
component: BBa_B0012
component: BBa_E0020
component: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_R0040
sequence annotation: BBa_E0030
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_E0020
sequence: https://synbiohub.org/public/igem/BBa_I15023_sequence/1

BBa_B0010
type: Component
persistent identity: component1516511
display id: component1516511
version: 1
top level: BBa_I15023
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1516504
display id: component1516504
version: 1
top level: BBa_I15023
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0030
type: Component
persistent identity: component1516506
display id: component1516506
version: 1
top level: BBa_I15023
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0012
type: Component
persistent identity: component1516481
display id: component1516481
version: 1
top level: BBa_I15023
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0020
type: Component
persistent identity: component1516466
display id: component1516466
version: 1
top level: BBa_I15023
access: public
definition: https://synbiohub.org/public/igem/BBa_E0020/1

BBa_B0012
type: Component
persistent identity: component1516521
display id: component1516521
version: 1
top level: BBa_I15023
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0040
type: Component
persistent identity: component1516456
display id: component1516456
version: 1
top level: BBa_I15023
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0034
type: Component
persistent identity: component1516464
display id: component1516464
version: 1
top level: BBa_I15023
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1516471
display id: component1516471
version: 1
top level: BBa_I15023
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0040
type: Component
persistent identity: component1516496
display id: component1516496
version: 1
top level: BBa_I15023
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1516464
display id: annotation1516464
version: 1
top level: BBa_I15023
location: https://synbiohub.org/public/igem/BBa_I15023/annotation1516464/range1516464/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I15023/annotation1516464/range1516464/1
type: Range
persistent identity: range1516464
display id: range1516464
version: 1
direction: direction/forward
top level: BBa_I15023
start: 63
end: 74
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1516456
display id: annotation1516456
version: 1
top level: BBa_I15023
location: https://synbiohub.org/public/igem/BBa_I15023/annotation1516456/range1516456/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I15023/annotation1516456/range1516456/1
type: Range
persistent identity: range1516456
display id: range1516456
version: 1
direction: direction/forward
top level: BBa_I15023
start: 1
end: 54
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation1516506
display id: annotation1516506
version: 1
top level: BBa_I15023
location: https://synbiohub.org/public/igem/BBa_I15023/annotation1516506/range1516506/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I15023/annotation1516506/range1516506/1
type: Range
persistent identity: range1516506
display id: range1516506
version: 1
direction: direction/forward
top level: BBa_I15023
start: 1029
end: 1751
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1516481
display id: annotation1516481
version: 1
top level: BBa_I15023
location: https://synbiohub.org/public/igem/BBa_I15023/annotation1516481/range1516481/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I15023/annotation1516481/range1516481/1
type: Range
persistent identity: range1516481
display id: range1516481
version: 1
direction: direction/forward
top level: BBa_I15023
start: 900
end: 940
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1516471
display id: annotation1516471
version: 1
top level: BBa_I15023
location: https://synbiohub.org/public/igem/BBa_I15023/annotation1516471/range1516471/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I15023/annotation1516471/range1516471/1
type: Range
persistent identity: range1516471
display id: range1516471
version: 1
direction: direction/forward
top level: BBa_I15023
start: 812
end: 891
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1516511
display id: annotation1516511
version: 1
top level: BBa_I15023
location: https://synbiohub.org/public/igem/BBa_I15023/annotation1516511/range1516511/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I15023/annotation1516511/range1516511/1
type: Range
persistent identity: range1516511
display id: range1516511
version: 1
direction: direction/forward
top level: BBa_I15023
start: 1760
end: 1839
orientation: inline

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation1516466
display id: annotation1516466
version: 1
top level: BBa_I15023
location: https://synbiohub.org/public/igem/BBa_I15023/annotation1516466/range1516466/1
component: BBa_E0020

https://synbiohub.org/public/igem/BBa_I15023/annotation1516466/range1516466/1
type: Range
persistent identity: range1516466
display id: range1516466
version: 1
direction: direction/forward
top level: BBa_I15023
start: 81
end: 803
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1516504
display id: annotation1516504
version: 1
top level: BBa_I15023
location: https://synbiohub.org/public/igem/BBa_I15023/annotation1516504/range1516504/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I15023/annotation1516504/range1516504/1
type: Range
persistent identity: range1516504
display id: range1516504
version: 1
direction: direction/forward
top level: BBa_I15023
start: 1011
end: 1022
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1516496
display id: annotation1516496
version: 1
top level: BBa_I15023
location: https://synbiohub.org/public/igem/BBa_I15023/annotation1516496/range1516496/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I15023/annotation1516496/range1516496/1
type: Range
persistent identity: range1516496
display id: range1516496
version: 1
direction: direction/forward
top level: BBa_I15023
start: 949
end: 1002
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1516521
display id: annotation1516521
version: 1
top level: BBa_I15023
location: https://synbiohub.org/public/igem/BBa_I15023/annotation1516521/range1516521/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I15023/annotation1516521/range1516521/1
type: Range
persistent identity: range1516521
display id: range1516521
version: 1
direction: direction/forward
top level: BBa_I15023
start: 1848
end: 1888
orientation: inline