//classic/generator/rc
type: Collection
persistent identity: classic generator rc
display id: classic_generator_rc
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K118006
member: rbs+crtY
member: BBa_K538103
member: rbs+crtIB
member: rbs+sam5
member: BBa_K118014
member: BBa_K118017
member: rbs+crtZ
member: BBa_K538100
member: BBa_K538102
member: rbs+COMT
member: rbs+crtE
member: BBa_K283048
member: BBa_K118013
member: rbs+sam8
member: BBa_K118007
member: BBa_K538104
member: rbs+LIMS1
member: BBa_K118005
member: BBa_K118018
member: BBa_K118004
member: BBa_K538101
member: rbs+ftsK

rbs+crtY
type: ComponentDefinition
persistent identity: BBa I742155
display id: BBa_I742155
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: crtY (lycopene cyclase) with native rbs
creator: Chris French
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:08:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
sample status: In stock
status: status/Available
bookmark: true
mutable description: crtY (lycopene cyclase) from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087. The native ribosome binding site is included. This is part of the carotenoid biosynthesis pathway, converting lycopene to beta-carotene.
mutable notes: This part was made as a single biobrick rather than separate rbs and coding sequence, hence the use of blunt ends in generating the final sequence.
mutable provenance: Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_I742154
component: BBa_I742153
sequence annotation: BBa_I742154
sequence annotation: BBa_I742153
sequence: https://synbiohub.org/public/igem/BBa_I742155_sequence/1

BBa_I742154
type: Component
persistent identity: component1953808
display id: component1953808
version: 1
top level: rbs+crtY
access: public
definition: https://synbiohub.org/public/igem/BBa_I742154/1

BBa_I742153
type: Component
persistent identity: component1953807
display id: component1953807
version: 1
top level: rbs+crtY
access: public
definition: https://synbiohub.org/public/igem/BBa_I742153/1

BBa_I742154
type: SequenceAnnotation
persistent identity: annotation1953808
display id: annotation1953808
version: 1
top level: rbs+crtY
location: https://synbiohub.org/public/igem/BBa_I742155/annotation1953808/range1953808/1
component: BBa_I742154

https://synbiohub.org/public/igem/BBa_I742155/annotation1953808/range1953808/1
type: Range
persistent identity: range1953808
display id: range1953808
version: 1
direction: direction/reverse
top level: rbs+crtY
start: 20
end: 1171
orientation: inline

BBa_I742153
type: SequenceAnnotation
persistent identity: annotation1953807
display id: annotation1953807
version: 1
top level: rbs+crtY
location: https://synbiohub.org/public/igem/BBa_I742155/annotation1953807/range1953807/1
component: BBa_I742153

https://synbiohub.org/public/igem/BBa_I742155/annotation1953807/range1953807/1
type: Range
persistent identity: range1953807
display id: range1953807
version: 1
direction: direction/reverse
top level: rbs+crtY
start: 1
end: 19
orientation: inline

BBa_K538102
type: ComponentDefinition
persistent identity: BBa K538102
display id: BBa_K538102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + SheDnaK
creator: Bas Stringer
created: 2011-09-07T11:00:00Z
modified: 2015-05-08T01:12:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _704_
m user id: 0
owner id: 9985
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K538002
component: BBa_B0034
sequence annotation: BBa_K538002
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K538102_sequence/1

BBa_K538002
type: Component
persistent identity: component2133895
display id: component2133895
version: 1
top level: BBa_K538102
access: public
definition: https://synbiohub.org/public/igem/BBa_K538002/1

BBa_B0034
type: Component
persistent identity: component2133887
display id: component2133887
version: 1
top level: BBa_K538102
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K538002
type: SequenceAnnotation
persistent identity: annotation2133895
display id: annotation2133895
version: 1
top level: BBa_K538102
location: https://synbiohub.org/public/igem/BBa_K538102/annotation2133895/range2133895/1
component: BBa_K538002

https://synbiohub.org/public/igem/BBa_K538102/annotation2133895/range2133895/1
type: Range
persistent identity: range2133895
display id: range2133895
version: 1
direction: direction/reverse
top level: BBa_K538102
start: 19
end: 1938
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2133887
display id: annotation2133887
version: 1
top level: BBa_K538102
location: https://synbiohub.org/public/igem/BBa_K538102/annotation2133887/range2133887/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K538102/annotation2133887/range2133887/1
type: Range
persistent identity: range2133887
display id: range2133887
version: 1
direction: direction/reverse
top level: BBa_K538102
start: 1
end: 12
orientation: inline

BBa_K283048
type: ComponentDefinition
persistent identity: BBa K283048
display id: BBa_K283048
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tetR(LVA) (Backbone: pSB1AK3)
creator: Shi Lei, W Ling , Nelson Chu
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:48Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _383_
m user id: 0
owner id: 5494
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is adapted from K283006 with a terminator added to the end of the device.
mutable notes: BBa_K283007
mutable provenance: The CI, terminator, pLambda promoter and the chez coding region are self designed The rbs, and the pLux promoter are from the registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: barcode
sequence annotation: SsrA
sequence annotation: BBa_C0040
sequence annotation: tetR
sequence: https://synbiohub.org/public/igem/BBa_K283048_sequence/1

barcode
type: SequenceAnnotation
persistent identity: annotation2057221
display id: annotation2057221
version: 1
top level: BBa_K283048
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K283048/annotation2057221/range2057221/1

https://synbiohub.org/public/igem/BBa_K283048/annotation2057221/range2057221/1
type: Range
persistent identity: range2057221
display id: range2057221
version: 1
direction: direction/reverse
top level: BBa_K283048
start: 661
end: 685
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation2057219
display id: annotation2057219
version: 1
top level: BBa_K283048
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K283048/annotation2057219/range2057219/1

https://synbiohub.org/public/igem/BBa_K283048/annotation2057219/range2057219/1
type: Range
persistent identity: range2057219
display id: range2057219
version: 1
direction: direction/forward
top level: BBa_K283048
start: 621
end: 654
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2057220
display id: annotation2057220
version: 1
top level: BBa_K283048
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K283048/annotation2057220/range2057220/1

https://synbiohub.org/public/igem/BBa_K283048/annotation2057220/range2057220/1
type: Range
persistent identity: range2057220
display id: range2057220
version: 1
direction: direction/reverse
top level: BBa_K283048
start: 1
end: 660
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation2057218
display id: annotation2057218
version: 1
top level: BBa_K283048
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K283048/annotation2057218/range2057218/1

https://synbiohub.org/public/igem/BBa_K283048/annotation2057218/range2057218/1
type: Range
persistent identity: range2057218
display id: range2057218
version: 1
direction: direction/forward
top level: BBa_K283048
start: 4
end: 620
orientation: inline

BBa_K538104
type: ComponentDefinition
persistent identity: BBa K538104
display id: BBa_K538104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + CspC
creator: Bas Stringer
created: 2011-09-07T11:00:00Z
modified: 2015-05-08T01:12:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _704_
m user id: 0
owner id: 9985
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0034
component: BBa_K538004
sequence annotation: BBa_B0034
sequence annotation: BBa_K538004
sequence: https://synbiohub.org/public/igem/BBa_K538104_sequence/1

BBa_B0034
type: Component
persistent identity: component2133904
display id: component2133904
version: 1
top level: BBa_K538104
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K538004
type: Component
persistent identity: component2133910
display id: component2133910
version: 1
top level: BBa_K538104
access: public
definition: https://synbiohub.org/public/igem/BBa_K538004/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2133904
display id: annotation2133904
version: 1
top level: BBa_K538104
location: https://synbiohub.org/public/igem/BBa_K538104/annotation2133904/range2133904/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K538104/annotation2133904/range2133904/1
type: Range
persistent identity: range2133904
display id: range2133904
version: 1
direction: direction/reverse
top level: BBa_K538104
start: 1
end: 12
orientation: inline

BBa_K538004
type: SequenceAnnotation
persistent identity: annotation2133910
display id: annotation2133910
version: 1
top level: BBa_K538104
location: https://synbiohub.org/public/igem/BBa_K538104/annotation2133910/range2133910/1
component: BBa_K538004

https://synbiohub.org/public/igem/BBa_K538104/annotation2133910/range2133910/1
type: Range
persistent identity: range2133910
display id: range2133910
version: 1
direction: direction/reverse
top level: BBa_K538104
start: 19
end: 213
orientation: inline

BBa_K118007
type: ComponentDefinition
persistent identity: BBa K118007
display id: BBa_K118007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rbs+appY (encoding a DNA-binding transcriptional activator)
creator: Andrew Hall
created: 2008-08-07T11:00:00Z
modified: 2015-05-08T01:09:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the coding sequence of appY from Escherichia coli JM109 preceded by a synthetic ribosome binding site (rbs). It encodes a transcriptional regulator related to anaerobic energy metabolism. Overexpression of the gene has been reported to result in enhanced lycopene production by E. coli (Kang et al., 2005).
mutable notes: No special considerations
mutable provenance: Escherichia coli JM109 genomic DNA.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K118001
component: BBa_J15001
sequence annotation: BBa_J15001
sequence annotation: BBa_K118001
sequence: https://synbiohub.org/public/igem/BBa_K118007_sequence/1

BBa_K118001
type: Component
persistent identity: component1970368
display id: component1970368
version: 1
top level: BBa_K118007
access: public
definition: https://synbiohub.org/public/igem/BBa_K118001/1

BBa_J15001
type: Component
persistent identity: component1970367
display id: component1970367
version: 1
top level: BBa_K118007
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation1970367
display id: annotation1970367
version: 1
top level: BBa_K118007
location: https://synbiohub.org/public/igem/BBa_K118007/annotation1970367/range1970367/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K118007/annotation1970367/range1970367/1
type: Range
persistent identity: range1970367
display id: range1970367
version: 1
direction: direction/reverse
top level: BBa_K118007
start: 1
end: 10
orientation: inline

BBa_K118001
type: SequenceAnnotation
persistent identity: annotation1970368
display id: annotation1970368
version: 1
top level: BBa_K118007
location: https://synbiohub.org/public/igem/BBa_K118007/annotation1970368/range1970368/1
component: BBa_K118001

https://synbiohub.org/public/igem/BBa_K118007/annotation1970368/range1970368/1
type: Range
persistent identity: range1970368
display id: range1970368
version: 1
direction: direction/reverse
top level: BBa_K118007
start: 17
end: 769
orientation: inline

BBa_K538100
type: ComponentDefinition
persistent identity: BBa K538100
display id: BBa_K538100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + Cpn10
creator: Bas Stringer
created: 2011-09-07T11:00:00Z
modified: 2015-05-08T01:12:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _704_
m user id: 0
owner id: 9985
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K538000
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K538000
sequence: https://synbiohub.org/public/igem/BBa_K538100_sequence/1

BBa_K538000
type: Component
persistent identity: component2133879
display id: component2133879
version: 1
top level: BBa_K538100
access: public
definition: https://synbiohub.org/public/igem/BBa_K538000/1

BBa_B0034
type: Component
persistent identity: component2133876
display id: component2133876
version: 1
top level: BBa_K538100
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2133876
display id: annotation2133876
version: 1
top level: BBa_K538100
location: https://synbiohub.org/public/igem/BBa_K538100/annotation2133876/range2133876/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K538100/annotation2133876/range2133876/1
type: Range
persistent identity: range2133876
display id: range2133876
version: 1
direction: direction/reverse
top level: BBa_K538100
start: 1
end: 12
orientation: inline

BBa_K538000
type: SequenceAnnotation
persistent identity: annotation2133879
display id: annotation2133879
version: 1
top level: BBa_K538100
location: https://synbiohub.org/public/igem/BBa_K538100/annotation2133879/range2133879/1
component: BBa_K538000

https://synbiohub.org/public/igem/BBa_K538100/annotation2133879/range2133879/1
type: Range
persistent identity: range2133879
display id: range2133879
version: 1
direction: direction/reverse
top level: BBa_K538100
start: 19
end: 315
orientation: inline

rbs+sam5
type: ComponentDefinition
persistent identity: BBa I742147
display id: BBa_I742147
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sam5 (coumarate hydroxylase) with native rbs.
creator: sarah hollingshead
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:08:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Sam5 (4-coumarate-3-hydroxylase) of the bacterium Saccharothrix espanaensis DSM 44229 with its native rbs.
mutable notes: This was made as a single biobrick, rather than separate rbs and coding sequence, hence the use of blunt ligation in generating this composite part.
mutable provenance: Saccharothrix espanaensis DSM 44229
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_I742145
component: BBa_I742141
sequence annotation: BBa_I742145
sequence annotation: BBa_I742141
sequence: https://synbiohub.org/public/igem/BBa_I742147_sequence/1

BBa_I742145
type: Component
persistent identity: component1953792
display id: component1953792
version: 1
top level: rbs+sam5
access: public
definition: https://synbiohub.org/public/igem/BBa_I742145/1

BBa_I742141
type: Component
persistent identity: component1953794
display id: component1953794
version: 1
top level: rbs+sam5
access: public
definition: https://synbiohub.org/public/igem/BBa_I742141/1

BBa_I742145
type: SequenceAnnotation
persistent identity: annotation1953792
display id: annotation1953792
version: 1
top level: rbs+sam5
location: https://synbiohub.org/public/igem/BBa_I742147/annotation1953792/range1953792/1
component: BBa_I742145

https://synbiohub.org/public/igem/BBa_I742147/annotation1953792/range1953792/1
type: Range
persistent identity: range1953792
display id: range1953792
version: 1
direction: direction/reverse
top level: rbs+sam5
start: 1
end: 16
orientation: inline

BBa_I742141
type: SequenceAnnotation
persistent identity: annotation1953794
display id: annotation1953794
version: 1
top level: rbs+sam5
location: https://synbiohub.org/public/igem/BBa_I742147/annotation1953794/range1953794/1
component: BBa_I742141

https://synbiohub.org/public/igem/BBa_I742147/annotation1953794/range1953794/1
type: Range
persistent identity: range1953794
display id: range1953794
version: 1
direction: direction/reverse
top level: rbs+sam5
start: 17
end: 1558
orientation: inline

BBa_K538101
type: ComponentDefinition
persistent identity: BBa K538101
display id: BBa_K538101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + Cpn60
creator: Bas Stringer
created: 2011-09-07T11:00:00Z
modified: 2015-05-08T01:12:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _704_
m user id: 0
owner id: 9985
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K538001
component: BBa_B0034
sequence annotation: BBa_K538001
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K538101_sequence/1

BBa_K538001
type: Component
persistent identity: component2133885
display id: component2133885
version: 1
top level: BBa_K538101
access: public
definition: https://synbiohub.org/public/igem/BBa_K538001/1

BBa_B0034
type: Component
persistent identity: component2133881
display id: component2133881
version: 1
top level: BBa_K538101
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K538001
type: SequenceAnnotation
persistent identity: annotation2133885
display id: annotation2133885
version: 1
top level: BBa_K538101
location: https://synbiohub.org/public/igem/BBa_K538101/annotation2133885/range2133885/1
component: BBa_K538001

https://synbiohub.org/public/igem/BBa_K538101/annotation2133885/range2133885/1
type: Range
persistent identity: range2133885
display id: range2133885
version: 1
direction: direction/reverse
top level: BBa_K538101
start: 19
end: 1668
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2133881
display id: annotation2133881
version: 1
top level: BBa_K538101
location: https://synbiohub.org/public/igem/BBa_K538101/annotation2133881/range2133881/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K538101/annotation2133881/range2133881/1
type: Range
persistent identity: range2133881
display id: range2133881
version: 1
direction: direction/reverse
top level: BBa_K538101
start: 1
end: 12
orientation: inline

rbs+sam8
type: ComponentDefinition
persistent identity: BBa I742148
display id: BBa_I742148
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sam8 (tyrosine ammonia lyase) with native rbs
creator: sarah hollingshead
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:08:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is sam8 (tyrosine ammonia lyase) of the bacterium Saccharothrix espanaensis DSM 44229 with its native ribosome binding site.
mutable notes: This part was made as a single biobrick rather than separate rbs and coding sequence, hence the use of blunt ligation in generating the final sequence.
mutable provenance: Saccharothrix espanaensis DSM 44229
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_I742146
component: BBa_I742142
sequence annotation: BBa_I742142
sequence annotation: BBa_I742146
sequence: https://synbiohub.org/public/igem/BBa_I742148_sequence/1

BBa_I742146
type: Component
persistent identity: component1953823
display id: component1953823
version: 1
top level: rbs+sam8
access: public
definition: https://synbiohub.org/public/igem/BBa_I742146/1

BBa_I742142
type: Component
persistent identity: component1953824
display id: component1953824
version: 1
top level: rbs+sam8
access: public
definition: https://synbiohub.org/public/igem/BBa_I742142/1

BBa_I742142
type: SequenceAnnotation
persistent identity: annotation1953824
display id: annotation1953824
version: 1
top level: rbs+sam8
location: https://synbiohub.org/public/igem/BBa_I742148/annotation1953824/range1953824/1
component: BBa_I742142

https://synbiohub.org/public/igem/BBa_I742148/annotation1953824/range1953824/1
type: Range
persistent identity: range1953824
display id: range1953824
version: 1
direction: direction/reverse
top level: rbs+sam8
start: 15
end: 1550
orientation: inline

BBa_I742146
type: SequenceAnnotation
persistent identity: annotation1953823
display id: annotation1953823
version: 1
top level: rbs+sam8
location: https://synbiohub.org/public/igem/BBa_I742148/annotation1953823/range1953823/1
component: BBa_I742146

https://synbiohub.org/public/igem/BBa_I742148/annotation1953823/range1953823/1
type: Range
persistent identity: range1953823
display id: range1953823
version: 1
direction: direction/reverse
top level: rbs+sam8
start: 1
end: 14
orientation: inline

rbs+COMT
type: ComponentDefinition
persistent identity: BBa I742109
display id: BBa_I742109
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: COMT gene with ribosome binding site
creator: sarah hollingshead
created: 2007-10-18T11:00:00Z
modified: 2015-08-31T04:08:02Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 1964
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: COMT (caffeic acid-O-methyl transferase) with ribosome binding site. Enzyme methylates -OH on C4 of the caffeic acid aromatic ring to produce ferulic acid. COMT is the third gene in the vanillin biosynthesis pathway,
mutable notes: Used COMT cDNA as genomic DNA contains introns
mutable provenance: Genes and Enzymes Involved in Caffeic Acid Biosynthesis in the Actinomycete Saccharothrix esanaensis, Berner M, et al, Journal of Bacteriology 188(7): 2666, (2006) Gowri G, et al, Molecular cloning and expression of alfalfa S-adenosyl-L-methionine: caffeic acid 3-0-methyltransferase, a key enzyme of lignin biosynthesis, Plant Physiology 97(1):7 (1991) Downregulation of Caffeic Acid 3-O-Methyltransferase and Caffeoyl CoA 3-O-Methyltransferase in Transgenic Alfalfa Impacts on Lignin Structure and Implications for the Biosynthesis of G and S Lignin, Guo D, et al, Plant Cell. January; 13(1): 73 (2001)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_I742107
component: BBa_J15001
sequence annotation: BBa_I742107
sequence annotation: BBa_J15001
sequence: https://synbiohub.org/public/igem/BBa_I742109_sequence/1

BBa_I742107
type: Component
persistent identity: component1949771
display id: component1949771
version: 1
top level: rbs+COMT
access: public
definition: https://synbiohub.org/public/igem/BBa_I742107/1

BBa_J15001
type: Component
persistent identity: component1949770
display id: component1949770
version: 1
top level: rbs+COMT
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_I742107
type: SequenceAnnotation
persistent identity: annotation1949771
display id: annotation1949771
version: 1
top level: rbs+COMT
location: https://synbiohub.org/public/igem/BBa_I742109/annotation1949771/range1949771/1
component: BBa_I742107

https://synbiohub.org/public/igem/BBa_I742109/annotation1949771/range1949771/1
type: Range
persistent identity: range1949771
display id: range1949771
version: 1
direction: direction/reverse
top level: rbs+COMT
start: 17
end: 1117
orientation: inline

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation1949770
display id: annotation1949770
version: 1
top level: rbs+COMT
location: https://synbiohub.org/public/igem/BBa_I742109/annotation1949770/range1949770/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_I742109/annotation1949770/range1949770/1
type: Range
persistent identity: range1949770
display id: range1949770
version: 1
direction: direction/reverse
top level: rbs+COMT
start: 1
end: 10
orientation: inline

rbs+crtZ
type: ComponentDefinition
persistent identity: BBa I742158
display id: BBa_I742158
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: crtZ (beta-carotene hydroxylase) with native rbs
creator: Chris French
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:08:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: crtZ (beta-carotene hydroxylase) from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087. Part of the carotenoid biosynthesis pathway. Converts beta-carotene to the yellow pigment zeaxnathin.
mutable notes: This part was made as a single biobrick rather than separate rbs and coding sequence, hence the use of blunt combination to generate the final sequence.
mutable provenance: Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_I742157
component: BBa_I742156
sequence annotation: BBa_I742157
sequence annotation: BBa_I742156
sequence: https://synbiohub.org/public/igem/BBa_I742158_sequence/1

BBa_I742157
type: Component
persistent identity: component1953815
display id: component1953815
version: 1
top level: rbs+crtZ
access: public
definition: https://synbiohub.org/public/igem/BBa_I742157/1

BBa_I742156
type: Component
persistent identity: component1953813
display id: component1953813
version: 1
top level: rbs+crtZ
access: public
definition: https://synbiohub.org/public/igem/BBa_I742156/1

BBa_I742157
type: SequenceAnnotation
persistent identity: annotation1953815
display id: annotation1953815
version: 1
top level: rbs+crtZ
location: https://synbiohub.org/public/igem/BBa_I742158/annotation1953815/range1953815/1
component: BBa_I742157

https://synbiohub.org/public/igem/BBa_I742158/annotation1953815/range1953815/1
type: Range
persistent identity: range1953815
display id: range1953815
version: 1
direction: direction/reverse
top level: rbs+crtZ
start: 17
end: 547
orientation: inline

BBa_I742156
type: SequenceAnnotation
persistent identity: annotation1953813
display id: annotation1953813
version: 1
top level: rbs+crtZ
location: https://synbiohub.org/public/igem/BBa_I742158/annotation1953813/range1953813/1
component: BBa_I742156

https://synbiohub.org/public/igem/BBa_I742158/annotation1953813/range1953813/1
type: Range
persistent identity: range1953813
display id: range1953813
version: 1
direction: direction/reverse
top level: rbs+crtZ
start: 1
end: 16
orientation: inline

BBa_K118004
type: ComponentDefinition
persistent identity: BBa K118004
display id: BBa_K118004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rbs+dxs (1-deoxyxylulose-5-phosphate synthase)
creator: Andrew Hall
created: 2008-08-07T11:00:00Z
modified: 2015-05-08T01:09:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the coding sequence of dxs from Escherichia coli JM109 with added ribosome binding site (rbs). It encodes 1-deoxyxylulose-5-phosphate synthase, thiamine-requiring, which catalyses the first step in the biosynthesis of terpenoids. Overexpression of dxs has been reported to increase yields of carotenoids and other terpenoids [add reference here].
mutable notes: No special considerations.
mutable provenance: Escherichia coli JM109 genomic DNA.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K118000
component: BBa_J15001
sequence annotation: BBa_J15001
sequence annotation: BBa_K118000
sequence: https://synbiohub.org/public/igem/BBa_K118004_sequence/1

BBa_K118000
type: Component
persistent identity: component1970354
display id: component1970354
version: 1
top level: BBa_K118004
access: public
definition: https://synbiohub.org/public/igem/BBa_K118000/1

BBa_J15001
type: Component
persistent identity: component1970353
display id: component1970353
version: 1
top level: BBa_K118004
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation1970353
display id: annotation1970353
version: 1
top level: BBa_K118004
location: https://synbiohub.org/public/igem/BBa_K118004/annotation1970353/range1970353/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K118004/annotation1970353/range1970353/1
type: Range
persistent identity: range1970353
display id: range1970353
version: 1
direction: direction/reverse
top level: BBa_K118004
start: 1
end: 10
orientation: inline

BBa_K118000
type: SequenceAnnotation
persistent identity: annotation1970354
display id: annotation1970354
version: 1
top level: BBa_K118004
location: https://synbiohub.org/public/igem/BBa_K118004/annotation1970354/range1970354/1
component: BBa_K118000

https://synbiohub.org/public/igem/BBa_K118004/annotation1970354/range1970354/1
type: Range
persistent identity: range1970354
display id: range1970354
version: 1
direction: direction/reverse
top level: BBa_K118004
start: 17
end: 1882
orientation: inline

BBa_K118018
type: ComponentDefinition
persistent identity: BBa K118018
display id: BBa_K118018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rbs+glgC16 (glgC with G336D substitution)
creator: Andrew Hall
created: 2008-10-05T11:00:00Z
modified: 2015-05-08T01:09:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the coding sequence of ''glgC'' (ADP-glucose pyrophosphorylase) from ''Escherichia coli'' JM109 with the substitution G336D, with added ribosome binding site (rbs). This mutation is known to cause increased activity of ADP-glucose pyrophosphorylase in the absence of the activator fructose 1,6-bisphosphate (FBP), high affinity for FBP and substrates lower affinity for the inhibitor AMP. (Leung ''et al.'', 1986; Meyer ''et al.'', 1988)
mutable notes: No special considerations
mutable provenance: ''Escherichia coli'' JM109 genomic DNA.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K118016
component: BBa_J15001
sequence annotation: BBa_J15001
sequence annotation: BBa_K118016
sequence: https://synbiohub.org/public/igem/BBa_K118018_sequence/1

BBa_K118016
type: Component
persistent identity: component1978637
display id: component1978637
version: 1
top level: BBa_K118018
access: public
definition: https://synbiohub.org/public/igem/BBa_K118016/1

BBa_J15001
type: Component
persistent identity: component1978633
display id: component1978633
version: 1
top level: BBa_K118018
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation1978633
display id: annotation1978633
version: 1
top level: BBa_K118018
location: https://synbiohub.org/public/igem/BBa_K118018/annotation1978633/range1978633/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K118018/annotation1978633/range1978633/1
type: Range
persistent identity: range1978633
display id: range1978633
version: 1
direction: direction/reverse
top level: BBa_K118018
start: 1
end: 10
orientation: inline

BBa_K118016
type: SequenceAnnotation
persistent identity: annotation1978637
display id: annotation1978637
version: 1
top level: BBa_K118018
location: https://synbiohub.org/public/igem/BBa_K118018/annotation1978637/range1978637/1
component: BBa_K118016

https://synbiohub.org/public/igem/BBa_K118018/annotation1978637/range1978637/1
type: Range
persistent identity: range1978637
display id: range1978637
version: 1
direction: direction/reverse
top level: BBa_K118018
start: 17
end: 1315
orientation: inline

BBa_K118017
type: ComponentDefinition
persistent identity: BBa K118017
display id: BBa_K118017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rbs+glgC (ADP-glucose pyrophosphorylase)
creator: Andrew Hall
created: 2008-10-05T11:00:00Z
modified: 2015-05-08T01:09:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the coding sequence of ''glgC'' from ''Escherichia coli'' JM109 with added ribosome binding site (rbs). It encodes ADP-glucose pyrophosphorylase, which catalyses Glucose 1-phosphate + ATP -> ADP-glucose + PPi, a step in the production of glycogen from glucose.
mutable notes: No special considerations.
mutable provenance: Escherichia coli JM109 genomic DNA.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K118015
component: BBa_J15001
sequence annotation: BBa_K118015
sequence annotation: BBa_J15001
sequence: https://synbiohub.org/public/igem/BBa_K118017_sequence/1

BBa_K118015
type: Component
persistent identity: component1978630
display id: component1978630
version: 1
top level: BBa_K118017
access: public
definition: https://synbiohub.org/public/igem/BBa_K118015/1

BBa_J15001
type: Component
persistent identity: component1978627
display id: component1978627
version: 1
top level: BBa_K118017
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_K118015
type: SequenceAnnotation
persistent identity: annotation1978630
display id: annotation1978630
version: 1
top level: BBa_K118017
location: https://synbiohub.org/public/igem/BBa_K118017/annotation1978630/range1978630/1
component: BBa_K118015

https://synbiohub.org/public/igem/BBa_K118017/annotation1978630/range1978630/1
type: Range
persistent identity: range1978630
display id: range1978630
version: 1
direction: direction/reverse
top level: BBa_K118017
start: 17
end: 1315
orientation: inline

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation1978627
display id: annotation1978627
version: 1
top level: BBa_K118017
location: https://synbiohub.org/public/igem/BBa_K118017/annotation1978627/range1978627/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K118017/annotation1978627/range1978627/1
type: Range
persistent identity: range1978627
display id: range1978627
version: 1
direction: direction/reverse
top level: BBa_K118017
start: 1
end: 10
orientation: inline

rbs+crtIB
type: ComponentDefinition
persistent identity: BBa I742161
display id: BBa_I742161
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: crtI (phytoene dehydrogenase) and crtB (phytoene synthase)
creator: Chris French
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:08:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: phytoene dehydrogenase and phytoene synthase from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087. Part of the carotenoid biosynthesis pathway. Note: the crtI coding sequence contains 2 PstI sites. In a later version of this part, these have been removed by site-directed mutagenesis but the functionality of the nutated gene has not been confirmed. This version still contains the two PstI sites. Reference: Misawa,N., Nakagawa,M., Kobayashi,K., Yamano,S., Izawa,Y., Nakamura,K. and Harashima,K. Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli. J. Bacteriol. 172 (12), 6704-6712 (1990)
mutable notes: Note: the crtI coding sequence contains 2 PstI sites. In a later version of this part, these have been removed by site-directed mutagenesis but the functionality of the nutated gene has not been confirmed. This version still contains the two PstI sites. Note also that the crtB coding sequence overlaps the crtI coding sequence; for simplicity, in this version, they have not been separated.
mutable provenance: Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_I742159
component: BBa_I742160
sequence annotation: BBa_I742159
sequence annotation: BBa_I742160
sequence: https://synbiohub.org/public/igem/BBa_I742161_sequence/1

BBa_I742159
type: Component
persistent identity: component1953826
display id: component1953826
version: 1
top level: rbs+crtIB
access: public
definition: https://synbiohub.org/public/igem/BBa_I742159/1

BBa_I742160
type: Component
persistent identity: component1953831
display id: component1953831
version: 1
top level: rbs+crtIB
access: public
definition: https://synbiohub.org/public/igem/BBa_I742160/1

BBa_I742159
type: SequenceAnnotation
persistent identity: annotation1953826
display id: annotation1953826
version: 1
top level: rbs+crtIB
location: https://synbiohub.org/public/igem/BBa_I742161/annotation1953826/range1953826/1
component: BBa_I742159

https://synbiohub.org/public/igem/BBa_I742161/annotation1953826/range1953826/1
type: Range
persistent identity: range1953826
display id: range1953826
version: 1
direction: direction/reverse
top level: rbs+crtIB
start: 1
end: 22
orientation: inline

BBa_I742160
type: SequenceAnnotation
persistent identity: annotation1953831
display id: annotation1953831
version: 1
top level: rbs+crtIB
location: https://synbiohub.org/public/igem/BBa_I742161/annotation1953831/range1953831/1
component: BBa_I742160

https://synbiohub.org/public/igem/BBa_I742161/annotation1953831/range1953831/1
type: Range
persistent identity: range1953831
display id: range1953831
version: 1
direction: direction/reverse
top level: rbs+crtIB
start: 23
end: 2430
orientation: inline

rbs+crtE
type: ComponentDefinition
persistent identity: BBa I742152
display id: BBa_I742152
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: crtE (geranylgeranyl pyrophosphate synthase) with native rbs.
creator: Chris French
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:08:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: crtE (geranylgeranyl pyrophosphate synthase) from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087. The native ribosome bindind site is included. This gene is part of the carotenoid biosynthesis pathway.
mutable notes: This part was made as a single biobrick rather than separate rbs and coding sequence, hence the use of blunt ligation in generating the final sequence.
mutable provenance: Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_I742151
component: BBa_I742150
sequence annotation: BBa_I742150
sequence annotation: BBa_I742151
sequence: https://synbiohub.org/public/igem/BBa_I742152_sequence/1

BBa_I742151
type: Component
persistent identity: component1953806
display id: component1953806
version: 1
top level: rbs+crtE
access: public
definition: https://synbiohub.org/public/igem/BBa_I742151/1

BBa_I742150
type: Component
persistent identity: component1953805
display id: component1953805
version: 1
top level: rbs+crtE
access: public
definition: https://synbiohub.org/public/igem/BBa_I742150/1

BBa_I742150
type: SequenceAnnotation
persistent identity: annotation1953805
display id: annotation1953805
version: 1
top level: rbs+crtE
location: https://synbiohub.org/public/igem/BBa_I742152/annotation1953805/range1953805/1
component: BBa_I742150

https://synbiohub.org/public/igem/BBa_I742152/annotation1953805/range1953805/1
type: Range
persistent identity: range1953805
display id: range1953805
version: 1
direction: direction/reverse
top level: rbs+crtE
start: 1
end: 42
orientation: inline

BBa_I742151
type: SequenceAnnotation
persistent identity: annotation1953806
display id: annotation1953806
version: 1
top level: rbs+crtE
location: https://synbiohub.org/public/igem/BBa_I742152/annotation1953806/range1953806/1
component: BBa_I742151

https://synbiohub.org/public/igem/BBa_I742152/annotation1953806/range1953806/1
type: Range
persistent identity: range1953806
display id: range1953806
version: 1
direction: direction/reverse
top level: rbs+crtE
start: 43
end: 954
orientation: inline

BBa_K118013
type: ComponentDefinition
persistent identity: BBa K118013
display id: BBa_K118013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rbs+crtY (lycopene B-cyclase)
creator: Andrew Hall
created: 2008-08-18T11:00:00Z
modified: 2015-05-08T01:09:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the coding sequence of ''crtY'' from ''Pantoea ananatis'' (formerly ''Erwinia uredovora'') (Accession number D90087). It encodes lycopene B-cyclase, part of the carotenoid biosynthesis pathway, which converts lycopene to B-carotene.
mutable notes: No special considerations
mutable provenance: The synthetic ribosome binding site was added by fusion PCR to the ''crtY'' gene, which was amplified from ''P. ananatis'' genomic DNA.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K118008
component: BBa_K118012
sequence annotation: BBa_K118012
sequence annotation: BBa_K118008
sequence: https://synbiohub.org/public/igem/BBa_K118013_sequence/1

BBa_K118008
type: Component
persistent identity: component1972438
display id: component1972438
version: 1
top level: BBa_K118013
access: public
definition: https://synbiohub.org/public/igem/BBa_K118008/1

BBa_K118012
type: Component
persistent identity: component1972437
display id: component1972437
version: 1
top level: BBa_K118013
access: public
definition: https://synbiohub.org/public/igem/BBa_K118012/1

BBa_K118012
type: SequenceAnnotation
persistent identity: annotation1972437
display id: annotation1972437
version: 1
top level: BBa_K118013
location: https://synbiohub.org/public/igem/BBa_K118013/annotation1972437/range1972437/1
component: BBa_K118012

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type: Range
persistent identity: range1972437
display id: range1972437
version: 1
direction: direction/reverse
top level: BBa_K118013
start: 1
end: 4
orientation: inline

BBa_K118008
type: SequenceAnnotation
persistent identity: annotation1972438
display id: annotation1972438
version: 1
top level: BBa_K118013
location: https://synbiohub.org/public/igem/BBa_K118013/annotation1972438/range1972438/1
component: BBa_K118008

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type: Range
persistent identity: range1972438
display id: range1972438
version: 1
direction: direction/reverse
top level: BBa_K118013
start: 11
end: 1162
orientation: inline

rbs+LIMS1
type: ComponentDefinition
persistent identity: BBa I742111
display id: BBa_I742111
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Limonene synthase gene with ribosome binding site
creator: sarah hollingshead
created: 2007-10-18T11:00:00Z
modified: 2015-08-31T04:08:02Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 1964
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Ribosome binding site attached to Limonene synthase gene. Limonene synthase enzyme produces (+)-limonene from farnesyl diphosphate (intermediate in mevlonate pathway). (+) Limonene is a component of lemon scent.
mutable notes: several codons were altered silently to remove several forbidden restriction sites. The N-terminal region (plastid targeting sequence) was removed, removing the first 51 amino acids and replacing codon 52 with the start codon ATG. LIMS cDNA was used as genomic DNA contains introns.
mutable provenance: Monoterpene biosynthesis in lemon (Citrus limon) cDNA isolation and functional analysis of four monoterpene synthases, J Lucker, et al, Eur. J. Biochem. 269: 3160???3171 (2002) Analysis of Lemon and Bergamot Essential Oils by HPLC with Microbore Columns, M Benincasa, et al, Chromatographia 30(5/6):271 (1990) Citrus fruit flavour and aroma biosynthesis, L Sharon-Asa, The Plant Journal, 36:664 (2003)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_I742110
component: BBa_J15001
sequence annotation: BBa_I742110
sequence annotation: BBa_J15001
sequence: https://synbiohub.org/public/igem/BBa_I742111_sequence/1

BBa_I742110
type: Component
persistent identity: component1955374
display id: component1955374
version: 1
top level: rbs+LIMS1
access: public
definition: https://synbiohub.org/public/igem/BBa_I742110/1

BBa_J15001
type: Component
persistent identity: component1955370
display id: component1955370
version: 1
top level: rbs+LIMS1
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_I742110
type: SequenceAnnotation
persistent identity: annotation1955374
display id: annotation1955374
version: 1
top level: rbs+LIMS1
location: https://synbiohub.org/public/igem/BBa_I742111/annotation1955374/range1955374/1
component: BBa_I742110

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type: Range
persistent identity: range1955374
display id: range1955374
version: 1
direction: direction/reverse
top level: rbs+LIMS1
start: 17
end: 1687
orientation: inline

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation1955370
display id: annotation1955370
version: 1
top level: rbs+LIMS1
location: https://synbiohub.org/public/igem/BBa_I742111/annotation1955370/range1955370/1
component: BBa_J15001

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type: Range
persistent identity: range1955370
display id: range1955370
version: 1
direction: direction/reverse
top level: rbs+LIMS1
start: 1
end: 10
orientation: inline

BBa_K538103
type: ComponentDefinition
persistent identity: BBa K538103
display id: BBa_K538103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + CspA
creator: Bas Stringer
created: 2011-09-07T11:00:00Z
modified: 2015-05-08T01:12:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _704_
m user id: 0
owner id: 9985
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0034
component: BBa_K538003
sequence annotation: BBa_K538003
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K538103_sequence/1

BBa_B0034
type: Component
persistent identity: component2133897
display id: component2133897
version: 1
top level: BBa_K538103
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K538003
type: Component
persistent identity: component2133902
display id: component2133902
version: 1
top level: BBa_K538103
access: public
definition: https://synbiohub.org/public/igem/BBa_K538003/1

BBa_K538003
type: SequenceAnnotation
persistent identity: annotation2133902
display id: annotation2133902
version: 1
top level: BBa_K538103
location: https://synbiohub.org/public/igem/BBa_K538103/annotation2133902/range2133902/1
component: BBa_K538003

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type: Range
persistent identity: range2133902
display id: range2133902
version: 1
direction: direction/reverse
top level: BBa_K538103
start: 19
end: 213
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2133897
display id: annotation2133897
version: 1
top level: BBa_K538103
location: https://synbiohub.org/public/igem/BBa_K538103/annotation2133897/range2133897/1
component: BBa_B0034

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type: Range
persistent identity: range2133897
display id: range2133897
version: 1
direction: direction/reverse
top level: BBa_K538103
start: 1
end: 12
orientation: inline

BBa_K118005
type: ComponentDefinition
persistent identity: BBa K118005
display id: BBa_K118005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rbs+crtI (phytoene dehydrogenase)
creator: Andrew Hall
created: 2008-08-07T11:00:00Z
modified: 2015-05-08T01:09:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the coding sequence of crtI from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087) preceded by an E. coli synthetic ribosome binding site (rbs). It encodes phytoene dehydrogenase, part of the carotenoid biosynthesis pathway, which converts Phytoene to Lycopene.
mutable notes: The native crtI sequence contains two internal PstI sites. Primers crtImutf1 and crtImutf2 were ordered so that the MABEL protocol (invented for this purpose) could be used to remove these sites. The mutagenic primers were as follows: * crtImutf1: gtt tac agt aag gtt gcc * crtImutf2: agt aac tct ctg ttt gtg c In both cases, the PstI site CTGCAG was converted to CTGCAA, a silent mutation.
mutable provenance: rbs: Escherichia coli JM109 genomic DNA. crtI: Pantoea ananatis genomic DNA
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_J15001
component: BBa_K118003
sequence annotation: BBa_J15001
sequence annotation: BBa_K118003
sequence: https://synbiohub.org/public/igem/BBa_K118005_sequence/1

BBa_J15001
type: Component
persistent identity: component1970357
display id: component1970357
version: 1
top level: BBa_K118005
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_K118003
type: Component
persistent identity: component1970360
display id: component1970360
version: 1
top level: BBa_K118005
access: public
definition: https://synbiohub.org/public/igem/BBa_K118003/1

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation1970357
display id: annotation1970357
version: 1
top level: BBa_K118005
location: https://synbiohub.org/public/igem/BBa_K118005/annotation1970357/range1970357/1
component: BBa_J15001

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type: Range
persistent identity: range1970357
display id: range1970357
version: 1
direction: direction/reverse
top level: BBa_K118005
start: 1
end: 10
orientation: inline

BBa_K118003
type: SequenceAnnotation
persistent identity: annotation1970360
display id: annotation1970360
version: 1
top level: BBa_K118005
location: https://synbiohub.org/public/igem/BBa_K118005/annotation1970360/range1970360/1
component: BBa_K118003

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type: Range
persistent identity: range1970360
display id: range1970360
version: 1
direction: direction/reverse
top level: BBa_K118005
start: 17
end: 1498
orientation: inline

rbs+ftsK
type: ComponentDefinition
persistent identity: BBa I742164
display id: BBa_I742164
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ftsK (cell division protein) of E. coli with native rbs
creator: Caroline Dahl and Xiaonan Wang
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:08:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is cell division gene ftsK of Escherichia coli JM109 (a K12 strain) with its native ribosome binding site. Note that this is the native sequence with a TTG start codon and two internal PstI sites.
mutable notes: Note that this is the native sequence with a TTG start codon and two internal PstI sites.
mutable provenance: Escherichia coli JM109 (a K12 strain).
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_I742163
component: BBa_I742137
sequence annotation: BBa_I742163
sequence annotation: BBa_I742137
sequence: https://synbiohub.org/public/igem/BBa_I742164_sequence/1

BBa_I742163
type: Component
persistent identity: component1954644
display id: component1954644
version: 1
top level: rbs+ftsK
access: public
definition: https://synbiohub.org/public/igem/BBa_I742163/1

BBa_I742137
type: Component
persistent identity: component1954646
display id: component1954646
version: 1
top level: rbs+ftsK
access: public
definition: https://synbiohub.org/public/igem/BBa_I742137/1

BBa_I742163
type: SequenceAnnotation
persistent identity: annotation1954644
display id: annotation1954644
version: 1
top level: rbs+ftsK
location: https://synbiohub.org/public/igem/BBa_I742164/annotation1954644/range1954644/1
component: BBa_I742163

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type: Range
persistent identity: range1954644
display id: range1954644
version: 1
direction: direction/reverse
top level: rbs+ftsK
start: 1
end: 26
orientation: inline

BBa_I742137
type: SequenceAnnotation
persistent identity: annotation1954646
display id: annotation1954646
version: 1
top level: rbs+ftsK
location: https://synbiohub.org/public/igem/BBa_I742164/annotation1954646/range1954646/1
component: BBa_I742137

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type: Range
persistent identity: range1954646
display id: range1954646
version: 1
direction: direction/reverse
top level: rbs+ftsK
start: 27
end: 4016
orientation: inline

BBa_K118006
type: ComponentDefinition
persistent identity: BBa K118006
display id: BBa_K118006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rbs+crtB (phytoene synthase)
creator: Andrew Hall
created: 2008-08-07T11:00:00Z
modified: 2015-05-08T01:09:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the coding sequence of crtB from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087) preceded by an E. coli synthetic ribosome binding site (rbs). It encodes phytoene synthase, part of the carotenoid biosynthesis pathway, which converts geranylgeranyl diphosphate to phytoene.
mutable notes: No special considerations.
mutable provenance: rbs: Escherichia coli JM109 genomic DNA. crtB: Pantoea ananatis genomic DNA
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K118002
component: BBa_J15001
sequence annotation: BBa_J15001
sequence annotation: BBa_K118002
sequence: https://synbiohub.org/public/igem/BBa_K118006_sequence/1

BBa_K118002
type: Component
persistent identity: component1970364
display id: component1970364
version: 1
top level: BBa_K118006
access: public
definition: https://synbiohub.org/public/igem/BBa_K118002/1

BBa_J15001
type: Component
persistent identity: component1970363
display id: component1970363
version: 1
top level: BBa_K118006
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation1970363
display id: annotation1970363
version: 1
top level: BBa_K118006
location: https://synbiohub.org/public/igem/BBa_K118006/annotation1970363/range1970363/1
component: BBa_J15001

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type: Range
persistent identity: range1970363
display id: range1970363
version: 1
direction: direction/reverse
top level: BBa_K118006
start: 1
end: 10
orientation: inline

BBa_K118002
type: SequenceAnnotation
persistent identity: annotation1970364
display id: annotation1970364
version: 1
top level: BBa_K118006
location: https://synbiohub.org/public/igem/BBa_K118006/annotation1970364/range1970364/1
component: BBa_K118002

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type: Range
persistent identity: range1970364
display id: range1970364
version: 1
direction: direction/reverse
top level: BBa_K118006
start: 17
end: 949
orientation: inline

BBa_K118014
type: ComponentDefinition
persistent identity: BBa K118014
display id: BBa_K118014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rbs+crtE (geranylgeranyl pyrophosphate synthase)
creator: Andrew Hall
created: 2008-08-18T11:00:00Z
modified: 2015-05-08T01:09:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the coding sequence of ''crtE'' from ''Pantoea ananatis'' (formerly ''Erwinia uredovora'') (Accession number D90087). It encodes geranylgeranyl pyrophosphate synthase, part of the carotenoid biosynthesis pathway, which converts farnesyl diphosphate to geranylgeranyl diphosphate.
mutable notes: The ''crtE'' BioBrick (BBa_I742151) had to be transferred from the non-compliant BABEL vector to a compliant pSB1A2 vector.
mutable provenance: The synthetic ribosome binding site was added by fusion PCR to the ''crtE'' gene, which was amplified from ''Pantoea ananatis'' DSMZ 30080 (ATCC 19321) genomic DNA.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_I742151
component: BBa_K118012
sequence annotation: BBa_I742151
sequence annotation: BBa_K118012
sequence: https://synbiohub.org/public/igem/BBa_K118014_sequence/1

BBa_I742151
type: Component
persistent identity: component1972440
display id: component1972440
version: 1
top level: BBa_K118014
access: public
definition: https://synbiohub.org/public/igem/BBa_I742151/1

BBa_K118012
type: Component
persistent identity: component1972439
display id: component1972439
version: 1
top level: BBa_K118014
access: public
definition: https://synbiohub.org/public/igem/BBa_K118012/1

BBa_I742151
type: SequenceAnnotation
persistent identity: annotation1972440
display id: annotation1972440
version: 1
top level: BBa_K118014
location: https://synbiohub.org/public/igem/BBa_K118014/annotation1972440/range1972440/1
component: BBa_I742151

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type: Range
persistent identity: range1972440
display id: range1972440
version: 1
direction: direction/reverse
top level: BBa_K118014
start: 11
end: 922
orientation: inline

BBa_K118012
type: SequenceAnnotation
persistent identity: annotation1972439
display id: annotation1972439
version: 1
top level: BBa_K118014
location: https://synbiohub.org/public/igem/BBa_K118014/annotation1972439/range1972439/1
component: BBa_K118012

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type: Range
persistent identity: range1972439
display id: range1972439
version: 1
direction: direction/reverse
top level: BBa_K118014
start: 1
end: 4
orientation: inline