//classic/generator/prct
type: Collection
persistent identity: classic generator prct
display id: classic_generator_prct
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K1399011
member: BBa_K1399016
member: BBa_I711001
member: SWOG
member: BBa_I769106
member: BBa_J69601
member: BBa_K1399023
member: BBa_I5000
member: SG
member: BBa_I5030
member: BBa_K1399014
member: BBa_I760001
member: BOG
member: BBa_K1399018
member: BBa_J45220
member: BBa_I5020
member: BBa_I718001
member: BBa_K1399019
member: BBa_K1399013
member: BBa_K283003
member: BBa_I731003
member: BBa_K283016
member: BBa_K283033
member: BBa_I711020
member: BBa_K283021
member: Pulsegen o
member: BBa_K1399012
member: BBa_J29006
member: B-carotene
member: BBa_I738001
member: Banana
member: BBa_K1399015
member: BBa_I711005
member: BBa_K1399017
member: BBa_I754001
member: BBa_I763002
member: BBa_K1399010
member: BBa_J69602
member: WOG
member: BBa_I711021
member: BBa_J01101
member: BBa_K1399022
member: BBa_K1399021
member: BBa_I744003
member: BBa_I711018
member: BBa_J45270
member: BBa_I5010
member: BBa_K1399020
member: BBa_K283000

BBa_I5030
type: ComponentDefinition
persistent identity: BBa I5030
display id: BBa_I5030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Olmecs: Inducible expression of P22 c2 repressor
creator: Olmecs 2003 IAP Team
created: 2003-11-27T12:00:00Z
modified: 2015-08-31T04:07:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0011
component: BBa_B0012
component: BBa_R0011
component: BBa_B0034
component: BBa_C0053
sequence annotation: BBa_R0011
sequence annotation: BBa_B0034
sequence annotation: BBa_C0053
sequence annotation: BBa_B0011
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I5030_sequence/1

BBa_B0011
type: Component
persistent identity: component2221131
display id: component2221131
version: 1
top level: BBa_I5030
access: public
definition: https://synbiohub.org/public/igem/BBa_B0011/1

BBa_B0012
type: Component
persistent identity: component2221127
display id: component2221127
version: 1
top level: BBa_I5030
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0011
type: Component
persistent identity: component2221115
display id: component2221115
version: 1
top level: BBa_I5030
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_B0034
type: Component
persistent identity: component2221121
display id: component2221121
version: 1
top level: BBa_I5030
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0053
type: Component
persistent identity: component2221123
display id: component2221123
version: 1
top level: BBa_I5030
access: public
definition: https://synbiohub.org/public/igem/BBa_C0053/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2221115
display id: annotation2221115
version: 1
top level: BBa_I5030
location: https://synbiohub.org/public/igem/BBa_I5030/annotation2221115/range2221115/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_I5030/annotation2221115/range2221115/1
type: Range
persistent identity: range2221115
display id: range2221115
version: 1
direction: direction/forward
top level: BBa_I5030
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2221121
display id: annotation2221121
version: 1
top level: BBa_I5030
location: https://synbiohub.org/public/igem/BBa_I5030/annotation2221121/range2221121/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I5030/annotation2221121/range2221121/1
type: Range
persistent identity: range2221121
display id: range2221121
version: 1
direction: direction/reverse
top level: BBa_I5030
start: 64
end: 75
orientation: inline

BBa_C0053
type: SequenceAnnotation
persistent identity: annotation2221123
display id: annotation2221123
version: 1
top level: BBa_I5030
location: https://synbiohub.org/public/igem/BBa_I5030/annotation2221123/range2221123/1
component: BBa_C0053

https://synbiohub.org/public/igem/BBa_I5030/annotation2221123/range2221123/1
type: Range
persistent identity: range2221123
display id: range2221123
version: 1
direction: direction/forward
top level: BBa_I5030
start: 82
end: 768
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2221131
display id: annotation2221131
version: 1
top level: BBa_I5030
location: https://synbiohub.org/public/igem/BBa_I5030/annotation2221131/range2221131/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_I5030/annotation2221131/range2221131/1
type: Range
persistent identity: range2221131
display id: range2221131
version: 1
direction: direction/forward
top level: BBa_I5030
start: 851
end: 896
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2221127
display id: annotation2221127
version: 1
top level: BBa_I5030
location: https://synbiohub.org/public/igem/BBa_I5030/annotation2221127/range2221127/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I5030/annotation2221127/range2221127/1
type: Range
persistent identity: range2221127
display id: range2221127
version: 1
direction: direction/forward
top level: BBa_I5030
start: 802
end: 842
orientation: inline

BOG
type: ComponentDefinition
persistent identity: BBa J45250
display id: BBa_J45250
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Stationary-phase-dependent banana odor generator
creator: Andr?? Green II
created: 2006-10-27T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 977
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part is an ATF1-1148 protein generator under control by the osmY stationary phase promoter. It is almost identical to J45270, except that: 1) The promoter is the truncated version of osmY and 2) it uses the B0030 RBS instead of B0032 (which is in J45270).
mutable notes: coming
mutable provenance: coming
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0010
component: BBa_J45014
component: BBa_B0030
component: BBa_B0012
component: BBa_J45992
sequence annotation: BBa_B0012
sequence annotation: BBa_B0030
sequence annotation: BBa_J45992
sequence annotation: BBa_B0010
sequence annotation: BBa_J45014
sequence: https://synbiohub.org/public/igem/BBa_J45250_sequence/1

BBa_B0010
type: Component
persistent identity: component1908081
display id: component1908081
version: 1
top level: BOG
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_J45014
type: Component
persistent identity: component1908080
display id: component1908080
version: 1
top level: BOG
access: public
definition: https://synbiohub.org/public/igem/BBa_J45014/1

BBa_B0030
type: Component
persistent identity: component1908074
display id: component1908074
version: 1
top level: BOG
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_B0012
type: Component
persistent identity: component1908083
display id: component1908083
version: 1
top level: BOG
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_J45992
type: Component
persistent identity: component1908072
display id: component1908072
version: 1
top level: BOG
access: public
definition: https://synbiohub.org/public/igem/BBa_J45992/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1908083
display id: annotation1908083
version: 1
top level: BOG
location: https://synbiohub.org/public/igem/BBa_J45250/annotation1908083/range1908083/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J45250/annotation1908083/range1908083/1
type: Range
persistent identity: range1908083
display id: range1908083
version: 1
direction: direction/forward
top level: BOG
start: 1906
end: 1946
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1908074
display id: annotation1908074
version: 1
top level: BOG
location: https://synbiohub.org/public/igem/BBa_J45250/annotation1908074/range1908074/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J45250/annotation1908074/range1908074/1
type: Range
persistent identity: range1908074
display id: range1908074
version: 1
direction: direction/forward
top level: BOG
start: 208
end: 222
orientation: inline

BBa_J45992
type: SequenceAnnotation
persistent identity: annotation1908072
display id: annotation1908072
version: 1
top level: BOG
location: https://synbiohub.org/public/igem/BBa_J45250/annotation1908072/range1908072/1
component: BBa_J45992

https://synbiohub.org/public/igem/BBa_J45250/annotation1908072/range1908072/1
type: Range
persistent identity: range1908072
display id: range1908072
version: 1
direction: direction/reverse
top level: BOG
start: 1
end: 199
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1908081
display id: annotation1908081
version: 1
top level: BOG
location: https://synbiohub.org/public/igem/BBa_J45250/annotation1908081/range1908081/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J45250/annotation1908081/range1908081/1
type: Range
persistent identity: range1908081
display id: range1908081
version: 1
direction: direction/forward
top level: BOG
start: 1818
end: 1897
orientation: inline

BBa_J45014
type: SequenceAnnotation
persistent identity: annotation1908080
display id: annotation1908080
version: 1
top level: BOG
location: https://synbiohub.org/public/igem/BBa_J45250/annotation1908080/range1908080/1
component: BBa_J45014

https://synbiohub.org/public/igem/BBa_J45250/annotation1908080/range1908080/1
type: Range
persistent identity: range1908080
display id: range1908080
version: 1
direction: direction/reverse
top level: BOG
start: 229
end: 1809
orientation: inline

BBa_J29006
type: ComponentDefinition
persistent identity: BBa J29006
display id: BBa_J29006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CrtB protein generator
creator: Mayu Ikezumi
created: 2006-08-01T11:00:00Z
modified: 2015-08-31T04:08:44Z
discontinued: false
dominant: false
group u list: _59_
m user id: 0
owner id: 892
owning group id: 59
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This device generates CrtB protein.
mutable notes: Arabibose promoter is used.
mutable provenance: This coding region comes from Erwinia uredovora.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0011
component: BBa_B0031
component: BBa_J29001
component: BBa_B0012
component: BBa_I0500
sequence annotation: BBa_B0031
sequence annotation: BBa_J29001
sequence annotation: BBa_B0012
sequence annotation: BBa_B0011
sequence annotation: BBa_I0500
sequence: https://synbiohub.org/public/igem/BBa_J29006_sequence/1

BBa_B0011
type: Component
persistent identity: component1892640
display id: component1892640
version: 1
top level: BBa_J29006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0011/1

BBa_B0031
type: Component
persistent identity: component1892632
display id: component1892632
version: 1
top level: BBa_J29006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0031/1

BBa_J29001
type: Component
persistent identity: component1892635
display id: component1892635
version: 1
top level: BBa_J29006
access: public
definition: https://synbiohub.org/public/igem/BBa_J29001/1

BBa_B0012
type: Component
persistent identity: component1892636
display id: component1892636
version: 1
top level: BBa_J29006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_I0500
type: Component
persistent identity: component1892630
display id: component1892630
version: 1
top level: BBa_J29006
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation1892632
display id: annotation1892632
version: 1
top level: BBa_J29006
location: https://synbiohub.org/public/igem/BBa_J29006/annotation1892632/range1892632/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_J29006/annotation1892632/range1892632/1
type: Range
persistent identity: range1892632
display id: range1892632
version: 1
direction: direction/reverse
top level: BBa_J29006
start: 1219
end: 1232
orientation: inline

BBa_J29001
type: SequenceAnnotation
persistent identity: annotation1892635
display id: annotation1892635
version: 1
top level: BBa_J29006
location: https://synbiohub.org/public/igem/BBa_J29006/annotation1892635/range1892635/1
component: BBa_J29001

https://synbiohub.org/public/igem/BBa_J29006/annotation1892635/range1892635/1
type: Range
persistent identity: range1892635
display id: range1892635
version: 1
direction: direction/reverse
top level: BBa_J29006
start: 1239
end: 2132
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1892636
display id: annotation1892636
version: 1
top level: BBa_J29006
location: https://synbiohub.org/public/igem/BBa_J29006/annotation1892636/range1892636/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J29006/annotation1892636/range1892636/1
type: Range
persistent identity: range1892636
display id: range1892636
version: 1
direction: direction/forward
top level: BBa_J29006
start: 2141
end: 2181
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation1892640
display id: annotation1892640
version: 1
top level: BBa_J29006
location: https://synbiohub.org/public/igem/BBa_J29006/annotation1892640/range1892640/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_J29006/annotation1892640/range1892640/1
type: Range
persistent identity: range1892640
display id: range1892640
version: 1
direction: direction/forward
top level: BBa_J29006
start: 2190
end: 2235
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation1892630
display id: annotation1892630
version: 1
top level: BBa_J29006
location: https://synbiohub.org/public/igem/BBa_J29006/annotation1892630/range1892630/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_J29006/annotation1892630/range1892630/1
type: Range
persistent identity: range1892630
display id: range1892630
version: 1
direction: direction/reverse
top level: BBa_J29006
start: 1
end: 1210
orientation: inline

BBa_I5010
type: ComponentDefinition
persistent identity: BBa I5010
display id: BBa_I5010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Olmecs: Inducible expression of Lambda cI repressor
creator: Olmecs 2003 IAP Team
created: 2003-11-27T12:00:00Z
modified: 2015-08-31T04:07:42Z
discontinued: false
dominant: true
experience: experience/Issues
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_R0011
component: BBa_B0011
component: BBa_B0012
component: BBa_C0051
component: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_C0051
sequence annotation: BBa_R0011
sequence annotation: BBa_B0034
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_I5010_sequence/1

BBa_R0011
type: Component
persistent identity: component2221081
display id: component2221081
version: 1
top level: BBa_I5010
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_B0011
type: Component
persistent identity: component2221096
display id: component2221096
version: 1
top level: BBa_I5010
access: public
definition: https://synbiohub.org/public/igem/BBa_B0011/1

BBa_B0012
type: Component
persistent identity: component2221092
display id: component2221092
version: 1
top level: BBa_I5010
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0051
type: Component
persistent identity: component2221091
display id: component2221091
version: 1
top level: BBa_I5010
access: public
definition: https://synbiohub.org/public/igem/BBa_C0051/1

BBa_B0034
type: Component
persistent identity: component2221087
display id: component2221087
version: 1
top level: BBa_I5010
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2221092
display id: annotation2221092
version: 1
top level: BBa_I5010
location: https://synbiohub.org/public/igem/BBa_I5010/annotation2221092/range2221092/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I5010/annotation2221092/range2221092/1
type: Range
persistent identity: range2221092
display id: range2221092
version: 1
direction: direction/forward
top level: BBa_I5010
start: 865
end: 905
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation2221091
display id: annotation2221091
version: 1
top level: BBa_I5010
location: https://synbiohub.org/public/igem/BBa_I5010/annotation2221091/range2221091/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_I5010/annotation2221091/range2221091/1
type: Range
persistent identity: range2221091
display id: range2221091
version: 1
direction: direction/reverse
top level: BBa_I5010
start: 82
end: 856
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2221081
display id: annotation2221081
version: 1
top level: BBa_I5010
location: https://synbiohub.org/public/igem/BBa_I5010/annotation2221081/range2221081/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_I5010/annotation2221081/range2221081/1
type: Range
persistent identity: range2221081
display id: range2221081
version: 1
direction: direction/forward
top level: BBa_I5010
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2221087
display id: annotation2221087
version: 1
top level: BBa_I5010
location: https://synbiohub.org/public/igem/BBa_I5010/annotation2221087/range2221087/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I5010/annotation2221087/range2221087/1
type: Range
persistent identity: range2221087
display id: range2221087
version: 1
direction: direction/reverse
top level: BBa_I5010
start: 64
end: 75
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2221096
display id: annotation2221096
version: 1
top level: BBa_I5010
location: https://synbiohub.org/public/igem/BBa_I5010/annotation2221096/range2221096/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_I5010/annotation2221096/range2221096/1
type: Range
persistent identity: range2221096
display id: range2221096
version: 1
direction: direction/forward
top level: BBa_I5010
start: 914
end: 959
orientation: inline

BBa_K1399014
type: ComponentDefinition
persistent identity: BBa K1399014
display id: BBa_K1399014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac-GFP(AAV)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Lactose/IPTG inducible promoter with GFP reporter tagged with AAV-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.
mutable notes: Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
mutable provenance: other biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_R0010
component: BBa_B0034
component: BBa_K1399005
component: BBa_B0015
sequence annotation: BBa_B0015
sequence annotation: BBa_R0010
sequence annotation: BBa_B0034
sequence annotation: BBa_K1399005
sequence: https://synbiohub.org/public/igem/BBa_K1399014_sequence/1

BBa_R0010
type: Component
persistent identity: component2384041
display id: component2384041
version: 1
top level: BBa_K1399014
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0034
type: Component
persistent identity: component2384049
display id: component2384049
version: 1
top level: BBa_K1399014
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1399005
type: Component
persistent identity: component2384055
display id: component2384055
version: 1
top level: BBa_K1399014
access: public
definition: https://synbiohub.org/public/igem/BBa_K1399005/1

BBa_B0015
type: Component
persistent identity: component2384062
display id: component2384062
version: 1
top level: BBa_K1399014
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2384062
display id: annotation2384062
version: 1
top level: BBa_K1399014
location: https://synbiohub.org/public/igem/BBa_K1399014/annotation2384062/range2384062/1
component: BBa_B0015

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type: Range
persistent identity: range2384062
display id: range2384062
version: 1
direction: direction/reverse
top level: BBa_K1399014
start: 988
end: 1116
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2384041
display id: annotation2384041
version: 1
top level: BBa_K1399014
location: https://synbiohub.org/public/igem/BBa_K1399014/annotation2384041/range2384041/1
component: BBa_R0010

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type: Range
persistent identity: range2384041
display id: range2384041
version: 1
direction: direction/forward
top level: BBa_K1399014
start: 1
end: 200
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2384049
display id: annotation2384049
version: 1
top level: BBa_K1399014
location: https://synbiohub.org/public/igem/BBa_K1399014/annotation2384049/range2384049/1
component: BBa_B0034

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type: Range
persistent identity: range2384049
display id: range2384049
version: 1
direction: direction/reverse
top level: BBa_K1399014
start: 209
end: 220
orientation: inline

BBa_K1399005
type: SequenceAnnotation
persistent identity: annotation2384055
display id: annotation2384055
version: 1
top level: BBa_K1399014
location: https://synbiohub.org/public/igem/BBa_K1399014/annotation2384055/range2384055/1
component: BBa_K1399005

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type: Range
persistent identity: range2384055
display id: range2384055
version: 1
direction: direction/reverse
top level: BBa_K1399014
start: 227
end: 979
orientation: inline

BBa_I744003
type: ComponentDefinition
persistent identity: BBa I744003
display id: BBa_I744003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: protein generator for biosynthesis of vanillin
creator: Beth Beason
created: 2007-05-25T11:00:00Z
modified: 2015-08-31T04:08:03Z
discontinued: true
dominant: false
experience: experience/None
group u list: _147_
m user id: 0
owner id: 678
owning group id: 75
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: Protein generator version of BBa-I744002. strong ribosomal binding site, promoter, and strong terminator
mutable notes: strong ribosome binding site..constitutive promoter...
mutable provenance: see Biobrick I744002
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0030
component: BBa_I744002
component: BBa_B0012
component: BBa_J23100
component: BBa_B0011
sequence annotation: BBa_B0011
sequence annotation: BBa_J23100
sequence annotation: BBa_B0012
sequence annotation: BBa_I744002
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_I744003_sequence/1

BBa_B0030
type: Component
persistent identity: component1933878
display id: component1933878
version: 1
top level: BBa_I744003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_I744002
type: Component
persistent identity: component1933880
display id: component1933880
version: 1
top level: BBa_I744003
access: public
definition: https://synbiohub.org/public/igem/BBa_I744002/1

BBa_B0012
type: Component
persistent identity: component1933881
display id: component1933881
version: 1
top level: BBa_I744003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_J23100
type: Component
persistent identity: component1933876
display id: component1933876
version: 1
top level: BBa_I744003
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_B0011
type: Component
persistent identity: component1933885
display id: component1933885
version: 1
top level: BBa_I744003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0011/1

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation1933885
display id: annotation1933885
version: 1
top level: BBa_I744003
location: https://synbiohub.org/public/igem/BBa_I744003/annotation1933885/range1933885/1
component: BBa_B0011

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type: Range
persistent identity: range1933885
display id: range1933885
version: 1
direction: direction/forward
top level: BBa_I744003
start: 1524
end: 1569
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation1933876
display id: annotation1933876
version: 1
top level: BBa_I744003
location: https://synbiohub.org/public/igem/BBa_I744003/annotation1933876/range1933876/1
component: BBa_J23100

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type: Range
persistent identity: range1933876
display id: range1933876
version: 1
direction: direction/reverse
top level: BBa_I744003
start: 1
end: 35
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1933881
display id: annotation1933881
version: 1
top level: BBa_I744003
location: https://synbiohub.org/public/igem/BBa_I744003/annotation1933881/range1933881/1
component: BBa_B0012

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type: Range
persistent identity: range1933881
display id: range1933881
version: 1
direction: direction/forward
top level: BBa_I744003
start: 1475
end: 1515
orientation: inline

BBa_I744002
type: SequenceAnnotation
persistent identity: annotation1933880
display id: annotation1933880
version: 1
top level: BBa_I744003
location: https://synbiohub.org/public/igem/BBa_I744003/annotation1933880/range1933880/1
component: BBa_I744002

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type: Range
persistent identity: range1933880
display id: range1933880
version: 1
direction: direction/reverse
top level: BBa_I744003
start: 67
end: 1466
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1933878
display id: annotation1933878
version: 1
top level: BBa_I744003
location: https://synbiohub.org/public/igem/BBa_I744003/annotation1933878/range1933878/1
component: BBa_B0030

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type: Range
persistent identity: range1933878
display id: range1933878
version: 1
direction: direction/forward
top level: BBa_I744003
start: 44
end: 58
orientation: inline

BBa_K1399020
type: ComponentDefinition
persistent identity: BBa K1399020
display id: BBa_K1399020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ptet-GFP(LVA)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Tetracycline/aTc inducible promoter with GFP reporter tagged with LVA-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.
mutable notes: Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
mutable provenance: other biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0034
component: BBa_B0015
component: BBa_R0040
component: BBa_K1399004
sequence annotation: BBa_B0034
sequence annotation: BBa_B0015
sequence annotation: BBa_K1399004
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_K1399020_sequence/1

BBa_B0034
type: Component
persistent identity: component2384245
display id: component2384245
version: 1
top level: BBa_K1399020
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0015
type: Component
persistent identity: component2384258
display id: component2384258
version: 1
top level: BBa_K1399020
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_R0040
type: Component
persistent identity: component2384239
display id: component2384239
version: 1
top level: BBa_K1399020
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_K1399004
type: Component
persistent identity: component2384251
display id: component2384251
version: 1
top level: BBa_K1399020
access: public
definition: https://synbiohub.org/public/igem/BBa_K1399004/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2384245
display id: annotation2384245
version: 1
top level: BBa_K1399020
location: https://synbiohub.org/public/igem/BBa_K1399020/annotation2384245/range2384245/1
component: BBa_B0034

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type: Range
persistent identity: range2384245
display id: range2384245
version: 1
direction: direction/reverse
top level: BBa_K1399020
start: 63
end: 74
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2384258
display id: annotation2384258
version: 1
top level: BBa_K1399020
location: https://synbiohub.org/public/igem/BBa_K1399020/annotation2384258/range2384258/1
component: BBa_B0015

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type: Range
persistent identity: range2384258
display id: range2384258
version: 1
direction: direction/reverse
top level: BBa_K1399020
start: 842
end: 970
orientation: inline

BBa_K1399004
type: SequenceAnnotation
persistent identity: annotation2384251
display id: annotation2384251
version: 1
top level: BBa_K1399020
location: https://synbiohub.org/public/igem/BBa_K1399020/annotation2384251/range2384251/1
component: BBa_K1399004

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type: Range
persistent identity: range2384251
display id: range2384251
version: 1
direction: direction/reverse
top level: BBa_K1399020
start: 81
end: 833
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2384239
display id: annotation2384239
version: 1
top level: BBa_K1399020
location: https://synbiohub.org/public/igem/BBa_K1399020/annotation2384239/range2384239/1
component: BBa_R0040

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type: Range
persistent identity: range2384239
display id: range2384239
version: 1
direction: direction/forward
top level: BBa_K1399020
start: 1
end: 54
orientation: inline

BBa_J01101
type: ComponentDefinition
persistent identity: BBa J01101
display id: BBa_J01101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [pTet][StrRBS][cI][DblTerm]
creator: Golden Bear
created: 2005-12-29T12:00:00Z
modified: 2015-08-31T04:08:13Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _13_
m user id: 0
owner id: 395
owning group id: 13
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: TEST [pTet][StrRBS][cI][DblTerm]
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0012
component: BBa_B0034
component: BBa_C0051
component: BBa_B0010
component: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_R0040
sequence annotation: BBa_B0010
sequence annotation: BBa_C0051
sequence: https://synbiohub.org/public/igem/BBa_J01101_sequence/1

BBa_B0012
type: Component
persistent identity: component1775756
display id: component1775756
version: 1
top level: BBa_J01101
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component1775730
display id: component1775730
version: 1
top level: BBa_J01101
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0051
type: Component
persistent identity: component1775740
display id: component1775740
version: 1
top level: BBa_J01101
access: public
definition: https://synbiohub.org/public/igem/BBa_C0051/1

BBa_B0010
type: Component
persistent identity: component1775746
display id: component1775746
version: 1
top level: BBa_J01101
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0040
type: Component
persistent identity: component1775722
display id: component1775722
version: 1
top level: BBa_J01101
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1775730
display id: annotation1775730
version: 1
top level: BBa_J01101
location: https://synbiohub.org/public/igem/BBa_J01101/annotation1775730/range1775730/1
component: BBa_B0034

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type: Range
persistent identity: range1775730
display id: range1775730
version: 1
direction: direction/forward
top level: BBa_J01101
start: 63
end: 74
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1775756
display id: annotation1775756
version: 1
top level: BBa_J01101
location: https://synbiohub.org/public/igem/BBa_J01101/annotation1775756/range1775756/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J01101/annotation1775756/range1775756/1
type: Range
persistent identity: range1775756
display id: range1775756
version: 1
direction: direction/forward
top level: BBa_J01101
start: 952
end: 992
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1775722
display id: annotation1775722
version: 1
top level: BBa_J01101
location: https://synbiohub.org/public/igem/BBa_J01101/annotation1775722/range1775722/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_J01101/annotation1775722/range1775722/1
type: Range
persistent identity: range1775722
display id: range1775722
version: 1
direction: direction/forward
top level: BBa_J01101
start: 1
end: 54
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1775746
display id: annotation1775746
version: 1
top level: BBa_J01101
location: https://synbiohub.org/public/igem/BBa_J01101/annotation1775746/range1775746/1
component: BBa_B0010

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type: Range
persistent identity: range1775746
display id: range1775746
version: 1
direction: direction/forward
top level: BBa_J01101
start: 864
end: 943
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation1775740
display id: annotation1775740
version: 1
top level: BBa_J01101
location: https://synbiohub.org/public/igem/BBa_J01101/annotation1775740/range1775740/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_J01101/annotation1775740/range1775740/1
type: Range
persistent identity: range1775740
display id: range1775740
version: 1
direction: direction/forward
top level: BBa_J01101
start: 81
end: 830
orientation: inline

SWOG
type: ComponentDefinition
persistent identity: BBa J45170
display id: BBa_J45170
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Stationary-phase-dependent wintergreen odor generator
creator: MIT IGEM 2006
created: 2006-08-09T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _84_
m user id: 0
owner id: 1147
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is the wintergreen generating device hooked up to a stationary phase promoter with RBS B0032.
mutable notes: Biobricks of osmY promoter and intermediate 45119.
mutable provenance: Still in development as SPP is not confirmed to work.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0032
component: BBa_J45993
component: BBa_J45004
component: BBa_B0010
component: BBa_B0012
sequence annotation: BBa_J45993
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_J45004
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_J45170_sequence/1

BBa_B0032
type: Component
persistent identity: component1894803
display id: component1894803
version: 1
top level: SWOG
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_J45993
type: Component
persistent identity: component1894801
display id: component1894801
version: 1
top level: SWOG
access: public
definition: https://synbiohub.org/public/igem/BBa_J45993/1

BBa_J45004
type: Component
persistent identity: component1894808
display id: component1894808
version: 1
top level: SWOG
access: public
definition: https://synbiohub.org/public/igem/BBa_J45004/1

BBa_B0010
type: Component
persistent identity: component1894809
display id: component1894809
version: 1
top level: SWOG
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1894811
display id: component1894811
version: 1
top level: SWOG
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_J45993
type: SequenceAnnotation
persistent identity: annotation1894801
display id: annotation1894801
version: 1
top level: SWOG
location: https://synbiohub.org/public/igem/BBa_J45170/annotation1894801/range1894801/1
component: BBa_J45993

https://synbiohub.org/public/igem/BBa_J45170/annotation1894801/range1894801/1
type: Range
persistent identity: range1894801
display id: range1894801
version: 1
direction: direction/reverse
top level: SWOG
start: 1
end: 57
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1894809
display id: annotation1894809
version: 1
top level: SWOG
location: https://synbiohub.org/public/igem/BBa_J45170/annotation1894809/range1894809/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J45170/annotation1894809/range1894809/1
type: Range
persistent identity: range1894809
display id: range1894809
version: 1
direction: direction/forward
top level: SWOG
start: 1167
end: 1246
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1894811
display id: annotation1894811
version: 1
top level: SWOG
location: https://synbiohub.org/public/igem/BBa_J45170/annotation1894811/range1894811/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J45170/annotation1894811/range1894811/1
type: Range
persistent identity: range1894811
display id: range1894811
version: 1
direction: direction/forward
top level: SWOG
start: 1255
end: 1295
orientation: inline

BBa_J45004
type: SequenceAnnotation
persistent identity: annotation1894808
display id: annotation1894808
version: 1
top level: SWOG
location: https://synbiohub.org/public/igem/BBa_J45170/annotation1894808/range1894808/1
component: BBa_J45004

https://synbiohub.org/public/igem/BBa_J45170/annotation1894808/range1894808/1
type: Range
persistent identity: range1894808
display id: range1894808
version: 1
direction: direction/reverse
top level: SWOG
start: 85
end: 1158
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1894803
display id: annotation1894803
version: 1
top level: SWOG
location: https://synbiohub.org/public/igem/BBa_J45170/annotation1894803/range1894803/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_J45170/annotation1894803/range1894803/1
type: Range
persistent identity: range1894803
display id: range1894803
version: 1
direction: direction/forward
top level: SWOG
start: 66
end: 78
orientation: inline

BBa_I5000
type: ComponentDefinition
persistent identity: BBa I5000
display id: BBa_I5000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Olmecs: Inducible expression of HK022 cI repressor
creator: Olmecs 2003 IAP Team
created: 2003-11-27T12:00:00Z
modified: 2015-08-31T04:07:41Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0012
component: BBa_R0011
component: BBa_C0050
component: BBa_B0011
component: BBa_B0034
sequence annotation: BBa_R0011
sequence annotation: BBa_C0050
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_I5000_sequence/1

BBa_B0012
type: Component
persistent identity: component2221075
display id: component2221075
version: 1
top level: BBa_I5000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0011
type: Component
persistent identity: component2221062
display id: component2221062
version: 1
top level: BBa_I5000
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_C0050
type: Component
persistent identity: component2221071
display id: component2221071
version: 1
top level: BBa_I5000
access: public
definition: https://synbiohub.org/public/igem/BBa_C0050/1

BBa_B0011
type: Component
persistent identity: component2221079
display id: component2221079
version: 1
top level: BBa_I5000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0011/1

BBa_B0034
type: Component
persistent identity: component2221068
display id: component2221068
version: 1
top level: BBa_I5000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2221062
display id: annotation2221062
version: 1
top level: BBa_I5000
location: https://synbiohub.org/public/igem/BBa_I5000/annotation2221062/range2221062/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_I5000/annotation2221062/range2221062/1
type: Range
persistent identity: range2221062
display id: range2221062
version: 1
direction: direction/forward
top level: BBa_I5000
start: 1
end: 54
orientation: inline

BBa_C0050
type: SequenceAnnotation
persistent identity: annotation2221071
display id: annotation2221071
version: 1
top level: BBa_I5000
location: https://synbiohub.org/public/igem/BBa_I5000/annotation2221071/range2221071/1
component: BBa_C0050

https://synbiohub.org/public/igem/BBa_I5000/annotation2221071/range2221071/1
type: Range
persistent identity: range2221071
display id: range2221071
version: 1
direction: direction/forward
top level: BBa_I5000
start: 82
end: 825
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2221068
display id: annotation2221068
version: 1
top level: BBa_I5000
location: https://synbiohub.org/public/igem/BBa_I5000/annotation2221068/range2221068/1
component: BBa_B0034

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type: Range
persistent identity: range2221068
display id: range2221068
version: 1
direction: direction/reverse
top level: BBa_I5000
start: 64
end: 75
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2221075
display id: annotation2221075
version: 1
top level: BBa_I5000
location: https://synbiohub.org/public/igem/BBa_I5000/annotation2221075/range2221075/1
component: BBa_B0012

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type: Range
persistent identity: range2221075
display id: range2221075
version: 1
direction: direction/forward
top level: BBa_I5000
start: 859
end: 899
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2221079
display id: annotation2221079
version: 1
top level: BBa_I5000
location: https://synbiohub.org/public/igem/BBa_I5000/annotation2221079/range2221079/1
component: BBa_B0011

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type: Range
persistent identity: range2221079
display id: range2221079
version: 1
direction: direction/forward
top level: BBa_I5000
start: 908
end: 953
orientation: inline

BBa_K1399022
type: ComponentDefinition
persistent identity: BBa K1399022
display id: BBa_K1399022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ptet-GFP(DAS+2)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Tetracycline/aTc inducible promoter with GFP reporter tagged with NYADAS-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.
mutable notes: Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
mutable provenance: other parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1399007
component: BBa_B0015
component: BBa_B0034
component: BBa_R0040
sequence annotation: BBa_B0015
sequence annotation: BBa_K1399007
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1399022_sequence/1

BBa_K1399007
type: Component
persistent identity: component2384291
display id: component2384291
version: 1
top level: BBa_K1399022
access: public
definition: https://synbiohub.org/public/igem/BBa_K1399007/1

BBa_B0015
type: Component
persistent identity: component2384298
display id: component2384298
version: 1
top level: BBa_K1399022
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0034
type: Component
persistent identity: component2384285
display id: component2384285
version: 1
top level: BBa_K1399022
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0040
type: Component
persistent identity: component2384279
display id: component2384279
version: 1
top level: BBa_K1399022
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2384298
display id: annotation2384298
version: 1
top level: BBa_K1399022
location: https://synbiohub.org/public/igem/BBa_K1399022/annotation2384298/range2384298/1
component: BBa_B0015

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type: Range
persistent identity: range2384298
display id: range2384298
version: 1
direction: direction/reverse
top level: BBa_K1399022
start: 848
end: 976
orientation: inline

BBa_K1399007
type: SequenceAnnotation
persistent identity: annotation2384291
display id: annotation2384291
version: 1
top level: BBa_K1399022
location: https://synbiohub.org/public/igem/BBa_K1399022/annotation2384291/range2384291/1
component: BBa_K1399007

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type: Range
persistent identity: range2384291
display id: range2384291
version: 1
direction: direction/reverse
top level: BBa_K1399022
start: 81
end: 839
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2384279
display id: annotation2384279
version: 1
top level: BBa_K1399022
location: https://synbiohub.org/public/igem/BBa_K1399022/annotation2384279/range2384279/1
component: BBa_R0040

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type: Range
persistent identity: range2384279
display id: range2384279
version: 1
direction: direction/forward
top level: BBa_K1399022
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2384285
display id: annotation2384285
version: 1
top level: BBa_K1399022
location: https://synbiohub.org/public/igem/BBa_K1399022/annotation2384285/range2384285/1
component: BBa_B0034

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type: Range
persistent identity: range2384285
display id: range2384285
version: 1
direction: direction/reverse
top level: BBa_K1399022
start: 63
end: 74
orientation: inline

BBa_I763002
type: ComponentDefinition
persistent identity: BBa I763002
display id: BBa_I763002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ECH protein generator
creator: Silvio Cavalcanti
created: 2007-06-26T11:00:00Z
modified: 2015-08-31T04:08:08Z
discontinued: true
dominant: false
experience: experience/None
group u list: _111_
m user id: 0
owner id: 1649
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: Per vanilla from.....
mutable notes: needs to be usede,,,,
mutable provenance: uses <partinfo>BBa_I769015</partinfo> as well as a promoter, RBS, and terminator
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0011
component: BBa_B0012
component: BBa_B0034
component: BBa_R0011
component: BBa_I769102
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_B0011
sequence annotation: BBa_R0011
sequence annotation: BBa_I769102
sequence: https://synbiohub.org/public/igem/BBa_I763002_sequence/1

BBa_B0011
type: Component
persistent identity: component1935774
display id: component1935774
version: 1
top level: BBa_I763002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0011/1

BBa_B0012
type: Component
persistent identity: component1935770
display id: component1935770
version: 1
top level: BBa_I763002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component1935765
display id: component1935765
version: 1
top level: BBa_I763002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0011
type: Component
persistent identity: component1935759
display id: component1935759
version: 1
top level: BBa_I763002
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_I769102
type: Component
persistent identity: component1935769
display id: component1935769
version: 1
top level: BBa_I763002
access: public
definition: https://synbiohub.org/public/igem/BBa_I769102/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1935770
display id: annotation1935770
version: 1
top level: BBa_I763002
location: https://synbiohub.org/public/igem/BBa_I763002/annotation1935770/range1935770/1
component: BBa_B0012

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type: Range
persistent identity: range1935770
display id: range1935770
version: 1
direction: direction/forward
top level: BBa_I763002
start: 924
end: 964
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1935765
display id: annotation1935765
version: 1
top level: BBa_I763002
location: https://synbiohub.org/public/igem/BBa_I763002/annotation1935765/range1935765/1
component: BBa_B0034

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type: Range
persistent identity: range1935765
display id: range1935765
version: 1
direction: direction/reverse
top level: BBa_I763002
start: 64
end: 75
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation1935774
display id: annotation1935774
version: 1
top level: BBa_I763002
location: https://synbiohub.org/public/igem/BBa_I763002/annotation1935774/range1935774/1
component: BBa_B0011

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type: Range
persistent identity: range1935774
display id: range1935774
version: 1
direction: direction/forward
top level: BBa_I763002
start: 973
end: 1018
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1935759
display id: annotation1935759
version: 1
top level: BBa_I763002
location: https://synbiohub.org/public/igem/BBa_I763002/annotation1935759/range1935759/1
component: BBa_R0011

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type: Range
persistent identity: range1935759
display id: range1935759
version: 1
direction: direction/forward
top level: BBa_I763002
start: 1
end: 54
orientation: inline

BBa_I769102
type: SequenceAnnotation
persistent identity: annotation1935769
display id: annotation1935769
version: 1
top level: BBa_I763002
location: https://synbiohub.org/public/igem/BBa_I763002/annotation1935769/range1935769/1
component: BBa_I769102

https://synbiohub.org/public/igem/BBa_I763002/annotation1935769/range1935769/1
type: Range
persistent identity: range1935769
display id: range1935769
version: 1
direction: direction/reverse
top level: BBa_I763002
start: 82
end: 915
orientation: inline

BBa_K283016
type: ComponentDefinition
persistent identity: BBa K283016
display id: BBa_K283016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLacI-rbs-cheZ_histag-terminators
creator: W Ling , Nelson Chu, Shi Lei
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _383_
m user id: 0
owner id: 5494
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The pLac promoter can be repressed by LacI protein. The cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement and is tagged by 6-Histidine tail.
mutable notes: BBa_K283016
mutable provenance: The chez-Histag coding region is self-designed The pLac promoter, rbs and the double terminator are from the registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0010
component: BBa_R0010
component: BBa_K283008
component: BBa_B0034
component: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_R0010
sequence annotation: BBa_K283008
sequence: https://synbiohub.org/public/igem/BBa_K283016_sequence/1

BBa_B0010
type: Component
persistent identity: component2035649
display id: component2035649
version: 1
top level: BBa_K283016
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0010
type: Component
persistent identity: component2035636
display id: component2035636
version: 1
top level: BBa_K283016
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_K283008
type: Component
persistent identity: component2035648
display id: component2035648
version: 1
top level: BBa_K283016
access: public
definition: https://synbiohub.org/public/igem/BBa_K283008/1

BBa_B0034
type: Component
persistent identity: component2035644
display id: component2035644
version: 1
top level: BBa_K283016
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component2035651
display id: component2035651
version: 1
top level: BBa_K283016
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2035644
display id: annotation2035644
version: 1
top level: BBa_K283016
location: https://synbiohub.org/public/igem/BBa_K283016/annotation2035644/range2035644/1
component: BBa_B0034

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type: Range
persistent identity: range2035644
display id: range2035644
version: 1
direction: direction/reverse
top level: BBa_K283016
start: 209
end: 220
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2035651
display id: annotation2035651
version: 1
top level: BBa_K283016
location: https://synbiohub.org/public/igem/BBa_K283016/annotation2035651/range2035651/1
component: BBa_B0012

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type: Range
persistent identity: range2035651
display id: range2035651
version: 1
direction: direction/forward
top level: BBa_K283016
start: 1036
end: 1076
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2035649
display id: annotation2035649
version: 1
top level: BBa_K283016
location: https://synbiohub.org/public/igem/BBa_K283016/annotation2035649/range2035649/1
component: BBa_B0010

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type: Range
persistent identity: range2035649
display id: range2035649
version: 1
direction: direction/forward
top level: BBa_K283016
start: 948
end: 1027
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2035636
display id: annotation2035636
version: 1
top level: BBa_K283016
location: https://synbiohub.org/public/igem/BBa_K283016/annotation2035636/range2035636/1
component: BBa_R0010

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type: Range
persistent identity: range2035636
display id: range2035636
version: 1
direction: direction/forward
top level: BBa_K283016
start: 1
end: 200
orientation: inline

BBa_K283008
type: SequenceAnnotation
persistent identity: annotation2035648
display id: annotation2035648
version: 1
top level: BBa_K283016
location: https://synbiohub.org/public/igem/BBa_K283016/annotation2035648/range2035648/1
component: BBa_K283008

https://synbiohub.org/public/igem/BBa_K283016/annotation2035648/range2035648/1
type: Range
persistent identity: range2035648
display id: range2035648
version: 1
direction: direction/reverse
top level: BBa_K283016
start: 227
end: 939
orientation: inline

BBa_K1399011
type: ComponentDefinition
persistent identity: BBa K1399011
display id: BBa_K1399011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac-RFP(LVA)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Lactose/IPTG inducible promoter with RBS, RFP reporter tagged with LVA-ssrA degradation tag and terminator. The tagged RFP is actively degraded within cell, thus provides better temporal resolution of red fluorescence and promoter activity. This part can be considered as improved version of BBa_J04451 as it does not contain StuI restriction site coding for additional 'RP' residues between RFP and LVA tag.
mutable notes: Part was assembled using BrickClip assembly (BBF RFC 104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
mutable provenance: other biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: BBa_R0010
sequence annotation: -10
sequence annotation: polya
sequence annotation: BBa_B0010
sequence annotation: -35
sequence annotation: BBa_B0015
sequence annotation: BBa_K1399001
sequence annotation: LVA-ssrA tag
sequence annotation: stop
sequence annotation: end of LacI coding region (inactive)
sequence annotation: stem_loop
sequence annotation: T7 TE
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: CAP binding site
sequence annotation: conserved
sequence annotation: start
sequence annotation: Barcode of BBa_E1010
sequence annotation: LacI binding site
sequence annotation: BBa_E1010 w/o stop
sequence: https://synbiohub.org/public/igem/BBa_K1399011_sequence/1

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2386289
display id: annotation2386289
version: 1
top level: BBa_K1399011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386289/range2386289/1

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type: Range
persistent identity: range2386289
display id: range2386289
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 1
end: 200
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2386293
display id: annotation2386293
version: 1
top level: BBa_K1399011
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386293/range2386293/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386293/range2386293/1
type: Range
persistent identity: range2386293
display id: range2386293
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 161
end: 166
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2386310
display id: annotation2386310
version: 1
top level: BBa_K1399011
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386310/range2386310/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386310/range2386310/1
type: Range
persistent identity: range2386310
display id: range2386310
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 1089
end: 1102
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2386306
display id: annotation2386306
version: 1
top level: BBa_K1399011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386306/range2386306/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386306/range2386306/1
type: Range
persistent identity: range2386306
display id: range2386306
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 974
end: 1053
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2386292
display id: annotation2386292
version: 1
top level: BBa_K1399011
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386292/range2386292/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386292/range2386292/1
type: Range
persistent identity: range2386292
display id: range2386292
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 137
end: 142
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2386305
display id: annotation2386305
version: 1
top level: BBa_K1399011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386305/range2386305/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386305/range2386305/1
type: Range
persistent identity: range2386305
display id: range2386305
version: 1
direction: direction/reverse
top level: BBa_K1399011
start: 974
end: 1102
orientation: inline

BBa_K1399001
type: SequenceAnnotation
persistent identity: annotation2386299
display id: annotation2386299
version: 1
top level: BBa_K1399011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386299/range2386299/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386299/range2386299/1
type: Range
persistent identity: range2386299
display id: range2386299
version: 1
direction: direction/reverse
top level: BBa_K1399011
start: 227
end: 940
orientation: inline

LVA-ssrA tag
type: SequenceAnnotation
persistent identity: annotation2386301
display id: annotation2386301
version: 1
top level: BBa_K1399011
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386301/range2386301/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386301/range2386301/1
type: Range
persistent identity: range2386301
display id: range2386301
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 902
end: 934
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2386303
display id: annotation2386303
version: 1
top level: BBa_K1399011
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386303/range2386303/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386303/range2386303/1
type: Range
persistent identity: range2386303
display id: range2386303
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 938
end: 940
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2386311
display id: annotation2386311
version: 1
top level: BBa_K1399011
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386311/range2386311/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386311/range2386311/1
type: Range
persistent identity: range2386311
display id: range2386311
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 1095
end: 1095
orientation: inline

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation2386290
display id: annotation2386290
version: 1
top level: BBa_K1399011
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386290/range2386290/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386290/range2386290/1
type: Range
persistent identity: range2386290
display id: range2386290
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 1
end: 88
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2386302
display id: annotation2386302
version: 1
top level: BBa_K1399011
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386302/range2386302/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386302/range2386302/1
type: Range
persistent identity: range2386302
display id: range2386302
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 935
end: 937
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2386307
display id: annotation2386307
version: 1
top level: BBa_K1399011
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386307/range2386307/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386307/range2386307/1
type: Range
persistent identity: range2386307
display id: range2386307
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 985
end: 1028
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2386309
display id: annotation2386309
version: 1
top level: BBa_K1399011
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386309/range2386309/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386309/range2386309/1
type: Range
persistent identity: range2386309
display id: range2386309
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 1069
end: 1088
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2386296
display id: annotation2386296
version: 1
top level: BBa_K1399011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386296/range2386296/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386296/range2386296/1
type: Range
persistent identity: range2386296
display id: range2386296
version: 1
direction: direction/reverse
top level: BBa_K1399011
start: 209
end: 220
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2386308
display id: annotation2386308
version: 1
top level: BBa_K1399011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386308/range2386308/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386308/range2386308/1
type: Range
persistent identity: range2386308
display id: range2386308
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 1062
end: 1102
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation2386291
display id: annotation2386291
version: 1
top level: BBa_K1399011
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386291/range2386291/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386291/range2386291/1
type: Range
persistent identity: range2386291
display id: range2386291
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 89
end: 126
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2386297
display id: annotation2386297
version: 1
top level: BBa_K1399011
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386297/range2386297/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386297/range2386297/1
type: Range
persistent identity: range2386297
display id: range2386297
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 213
end: 216
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2386298
display id: annotation2386298
version: 1
top level: BBa_K1399011
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386298/range2386298/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386298/range2386298/1
type: Range
persistent identity: range2386298
display id: range2386298
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 227
end: 229
orientation: inline

Barcode of BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2386304
display id: annotation2386304
version: 1
top level: BBa_K1399011
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386304/range2386304/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386304/range2386304/1
type: Range
persistent identity: range2386304
display id: range2386304
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 941
end: 965
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation2386294
display id: annotation2386294
version: 1
top level: BBa_K1399011
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386294/range2386294/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386294/range2386294/1
type: Range
persistent identity: range2386294
display id: range2386294
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 166
end: 200
orientation: inline

BBa_E1010 w/o stop
type: SequenceAnnotation
persistent identity: annotation2386300
display id: annotation2386300
version: 1
top level: BBa_K1399011
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386300/range2386300/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386300/range2386300/1
type: Range
persistent identity: range2386300
display id: range2386300
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 230
end: 901
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2386295
display id: annotation2386295
version: 1
top level: BBa_K1399011
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386295/range2386295/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386295/range2386295/1
type: Range
persistent identity: range2386295
display id: range2386295
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 173
end: 173
orientation: inline

BBa_K283021
type: ComponentDefinition
persistent identity: BBa K283021
display id: BBa_K283021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTet-rbs-cheZ-termintors
creator: Liu Chenli
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _383_
m user id: 0
owner id: 5494
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The chez is constitutively expressed under the pTet promoter. The pTet promoter can be repressed by TetR repressor protein. The cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement.
mutable notes: BBa_K283021
mutable provenance: The chez coding region is self-designed The pTet promoter, rbs and the terminator are from the registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_B0010
component: BBa_B0012
component: BBa_R0040
component: BBa_B0034
component: BBa_K094100
sequence annotation: BBa_B0034
sequence annotation: BBa_K094100
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_K283021_sequence/1

BBa_B0010
type: Component
persistent identity: component2035693
display id: component2035693
version: 1
top level: BBa_K283021
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component2035695
display id: component2035695
version: 1
top level: BBa_K283021
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0040
type: Component
persistent identity: component2035684
display id: component2035684
version: 1
top level: BBa_K283021
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0034
type: Component
persistent identity: component2035690
display id: component2035690
version: 1
top level: BBa_K283021
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K094100
type: Component
persistent identity: component2035692
display id: component2035692
version: 1
top level: BBa_K283021
access: public
definition: https://synbiohub.org/public/igem/BBa_K094100/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2035690
display id: annotation2035690
version: 1
top level: BBa_K283021
location: https://synbiohub.org/public/igem/BBa_K283021/annotation2035690/range2035690/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K283021/annotation2035690/range2035690/1
type: Range
persistent identity: range2035690
display id: range2035690
version: 1
direction: direction/reverse
top level: BBa_K283021
start: 63
end: 74
orientation: inline

BBa_K094100
type: SequenceAnnotation
persistent identity: annotation2035692
display id: annotation2035692
version: 1
top level: BBa_K283021
location: https://synbiohub.org/public/igem/BBa_K283021/annotation2035692/range2035692/1
component: BBa_K094100

https://synbiohub.org/public/igem/BBa_K283021/annotation2035692/range2035692/1
type: Range
persistent identity: range2035692
display id: range2035692
version: 1
direction: direction/reverse
top level: BBa_K283021
start: 81
end: 775
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2035695
display id: annotation2035695
version: 1
top level: BBa_K283021
location: https://synbiohub.org/public/igem/BBa_K283021/annotation2035695/range2035695/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K283021/annotation2035695/range2035695/1
type: Range
persistent identity: range2035695
display id: range2035695
version: 1
direction: direction/forward
top level: BBa_K283021
start: 872
end: 912
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2035693
display id: annotation2035693
version: 1
top level: BBa_K283021
location: https://synbiohub.org/public/igem/BBa_K283021/annotation2035693/range2035693/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K283021/annotation2035693/range2035693/1
type: Range
persistent identity: range2035693
display id: range2035693
version: 1
direction: direction/forward
top level: BBa_K283021
start: 784
end: 863
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2035684
display id: annotation2035684
version: 1
top level: BBa_K283021
location: https://synbiohub.org/public/igem/BBa_K283021/annotation2035684/range2035684/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K283021/annotation2035684/range2035684/1
type: Range
persistent identity: range2035684
display id: range2035684
version: 1
direction: direction/forward
top level: BBa_K283021
start: 1
end: 54
orientation: inline

BBa_J69602
type: ComponentDefinition
persistent identity: BBa J69602
display id: BBa_J69602
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TetR-repressible CI Generator
creator: James Brown
created: 2007-03-28T11:00:00Z
modified: 2015-05-08T01:08:19Z
discontinued: false
dominant: true
group u list: _101_
m user id: 0
owner id: 740
owning group id: 101
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: TetR-repressible CI Generator
mutable notes: NA
mutable provenance: Existing Reg Parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_R0040
component: BBa_P0455
sequence annotation: BBa_P0455
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_J69602_sequence/1

BBa_R0040
type: Component
persistent identity: component2252517
display id: component2252517
version: 1
top level: BBa_J69602
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_P0455
type: Component
persistent identity: component2252534
display id: component2252534
version: 1
top level: BBa_J69602
access: public
definition: https://synbiohub.org/public/igem/BBa_P0455/1

BBa_P0455
type: SequenceAnnotation
persistent identity: annotation2252534
display id: annotation2252534
version: 1
top level: BBa_J69602
location: https://synbiohub.org/public/igem/BBa_J69602/annotation2252534/range2252534/1
component: BBa_P0455

https://synbiohub.org/public/igem/BBa_J69602/annotation2252534/range2252534/1
type: Range
persistent identity: range2252534
display id: range2252534
version: 1
direction: direction/reverse
top level: BBa_J69602
start: 63
end: 958
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2252517
display id: annotation2252517
version: 1
top level: BBa_J69602
location: https://synbiohub.org/public/igem/BBa_J69602/annotation2252517/range2252517/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_J69602/annotation2252517/range2252517/1
type: Range
persistent identity: range2252517
display id: range2252517
version: 1
direction: direction/forward
top level: BBa_J69602
start: 1
end: 54
orientation: inline

BBa_J69601
type: ComponentDefinition
persistent identity: BBa J69601
display id: BBa_J69601
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Arabinose-inducible CI Generator
creator: James Brown
created: 2007-03-28T11:00:00Z
modified: 2015-05-08T01:08:19Z
discontinued: false
dominant: false
group u list: _101_
m user id: 0
owner id: 740
owning group id: 101
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Arabinose-inducible CI Generator
mutable notes: NA
mutable provenance: Existing Reg Parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_R0080
component: BBa_P0455
sequence annotation: BBa_P0455
sequence annotation: BBa_R0080
sequence: https://synbiohub.org/public/igem/BBa_J69601_sequence/1

BBa_R0080
type: Component
persistent identity: component2252503
display id: component2252503
version: 1
top level: BBa_J69601
access: public
definition: https://synbiohub.org/public/igem/BBa_R0080/1

BBa_P0455
type: Component
persistent identity: component2252516
display id: component2252516
version: 1
top level: BBa_J69601
access: public
definition: https://synbiohub.org/public/igem/BBa_P0455/1

BBa_P0455
type: SequenceAnnotation
persistent identity: annotation2252516
display id: annotation2252516
version: 1
top level: BBa_J69601
location: https://synbiohub.org/public/igem/BBa_J69601/annotation2252516/range2252516/1
component: BBa_P0455

https://synbiohub.org/public/igem/BBa_J69601/annotation2252516/range2252516/1
type: Range
persistent identity: range2252516
display id: range2252516
version: 1
direction: direction/reverse
top level: BBa_J69601
start: 158
end: 1053
orientation: inline

BBa_R0080
type: SequenceAnnotation
persistent identity: annotation2252503
display id: annotation2252503
version: 1
top level: BBa_J69601
location: https://synbiohub.org/public/igem/BBa_J69601/annotation2252503/range2252503/1
component: BBa_R0080

https://synbiohub.org/public/igem/BBa_J69601/annotation2252503/range2252503/1
type: Range
persistent identity: range2252503
display id: range2252503
version: 1
direction: direction/reverse
top level: BBa_J69601
start: 1
end: 149
orientation: inline

BBa_K1399010
type: ComponentDefinition
persistent identity: BBa K1399010
display id: BBa_K1399010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac-RFP(AAV)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Lactose/IPTG inducible promoter wuth RFP reporter tagged with AAV-ssrA degradation tag followed by terminator. The tagged RFP is actively degraded within cell, thus provides better temporal resolution of red fluorescence and promoter activity. Part was assembled using BrickClip assembly (BBF RFC 104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1] Reference [1] Trubitsyna, M., Michlewski, G., Cai, Y., Elfick, A. & French, C. E. PaperClip: rapid multi-part DNA assembly from existing libraries. Nucleic Acids Res. 1???8 (2014). doi:10.1093/nar/gku829
mutable notes: no
mutable provenance: Other biobrick parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: conserved
sequence annotation: start
sequence annotation: T7 TE
sequence annotation: end of LacI coding region (inactive)
sequence annotation: stop
sequence annotation: AAV-ssrA tag
sequence annotation: BBa_B0015
sequence annotation: BBa_B0012
sequence annotation: polya
sequence annotation: BBa_B0010
sequence annotation: BBa_E1010 w/o stop
sequence annotation: BBa_R0010
sequence annotation: barcode from BBa_E1010
sequence annotation: LacI binding site
sequence annotation: BBa_B0034
sequence annotation: CAP binding site
sequence annotation: BBa_K1399000
sequence annotation: -10
sequence annotation: -35
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_K1399010_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation2383940
display id: annotation2383940
version: 1
top level: BBa_K1399010
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383940/range2383940/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383940/range2383940/1
type: Range
persistent identity: range2383940
display id: range2383940
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 213
end: 216
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2383939
display id: annotation2383939
version: 1
top level: BBa_K1399010
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383939/range2383939/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383939/range2383939/1
type: Range
persistent identity: range2383939
display id: range2383939
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 173
end: 173
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2383951
display id: annotation2383951
version: 1
top level: BBa_K1399010
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383951/range2383951/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383951/range2383951/1
type: Range
persistent identity: range2383951
display id: range2383951
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 1069
end: 1088
orientation: inline

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation2383934
display id: annotation2383934
version: 1
top level: BBa_K1399010
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383934/range2383934/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383934/range2383934/1
type: Range
persistent identity: range2383934
display id: range2383934
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 1
end: 88
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383945
display id: annotation2383945
version: 1
top level: BBa_K1399010
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383945/range2383945/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383945/range2383945/1
type: Range
persistent identity: range2383945
display id: range2383945
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 935
end: 937
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383953
display id: annotation2383953
version: 1
top level: BBa_K1399010
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383953/range2383953/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383953/range2383953/1
type: Range
persistent identity: range2383953
display id: range2383953
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 1095
end: 1095
orientation: inline

AAV-ssrA tag
type: SequenceAnnotation
persistent identity: annotation2383944
display id: annotation2383944
version: 1
top level: BBa_K1399010
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383944/range2383944/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383944/range2383944/1
type: Range
persistent identity: range2383944
display id: range2383944
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 902
end: 934
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2383954
display id: annotation2383954
version: 1
top level: BBa_K1399010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383954/range2383954/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383954/range2383954/1
type: Range
persistent identity: range2383954
display id: range2383954
version: 1
direction: direction/reverse
top level: BBa_K1399010
start: 974
end: 1102
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2383950
display id: annotation2383950
version: 1
top level: BBa_K1399010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383950/range2383950/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383950/range2383950/1
type: Range
persistent identity: range2383950
display id: range2383950
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 1062
end: 1102
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2383952
display id: annotation2383952
version: 1
top level: BBa_K1399010
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383952/range2383952/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383952/range2383952/1
type: Range
persistent identity: range2383952
display id: range2383952
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 1089
end: 1102
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2383948
display id: annotation2383948
version: 1
top level: BBa_K1399010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383948/range2383948/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383948/range2383948/1
type: Range
persistent identity: range2383948
display id: range2383948
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 974
end: 1053
orientation: inline

BBa_E1010 w/o stop
type: SequenceAnnotation
persistent identity: annotation2383943
display id: annotation2383943
version: 1
top level: BBa_K1399010
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383943/range2383943/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383943/range2383943/1
type: Range
persistent identity: range2383943
display id: range2383943
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 230
end: 901
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2383933
display id: annotation2383933
version: 1
top level: BBa_K1399010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383933/range2383933/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383933/range2383933/1
type: Range
persistent identity: range2383933
display id: range2383933
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 1
end: 200
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383946
display id: annotation2383946
version: 1
top level: BBa_K1399010
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383946/range2383946/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383946/range2383946/1
type: Range
persistent identity: range2383946
display id: range2383946
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 938
end: 940
orientation: inline

barcode from BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2386257
display id: annotation2386257
version: 1
top level: BBa_K1399010
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2386257/range2386257/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2386257/range2386257/1
type: Range
persistent identity: range2386257
display id: range2386257
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 941
end: 965
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation2383938
display id: annotation2383938
version: 1
top level: BBa_K1399010
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383938/range2383938/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383938/range2383938/1
type: Range
persistent identity: range2383938
display id: range2383938
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 166
end: 200
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2383941
display id: annotation2383941
version: 1
top level: BBa_K1399010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383941/range2383941/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383941/range2383941/1
type: Range
persistent identity: range2383941
display id: range2383941
version: 1
direction: direction/reverse
top level: BBa_K1399010
start: 209
end: 220
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation2383935
display id: annotation2383935
version: 1
top level: BBa_K1399010
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383935/range2383935/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383935/range2383935/1
type: Range
persistent identity: range2383935
display id: range2383935
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 89
end: 126
orientation: inline

BBa_K1399000
type: SequenceAnnotation
persistent identity: annotation2383947
display id: annotation2383947
version: 1
top level: BBa_K1399010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383947/range2383947/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383947/range2383947/1
type: Range
persistent identity: range2383947
display id: range2383947
version: 1
direction: direction/reverse
top level: BBa_K1399010
start: 227
end: 940
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2383937
display id: annotation2383937
version: 1
top level: BBa_K1399010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383937/range2383937/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383937/range2383937/1
type: Range
persistent identity: range2383937
display id: range2383937
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 161
end: 166
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2383936
display id: annotation2383936
version: 1
top level: BBa_K1399010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383936/range2383936/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383936/range2383936/1
type: Range
persistent identity: range2383936
display id: range2383936
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 137
end: 142
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2383949
display id: annotation2383949
version: 1
top level: BBa_K1399010
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383949/range2383949/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383949/range2383949/1
type: Range
persistent identity: range2383949
display id: range2383949
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 985
end: 1028
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2383942
display id: annotation2383942
version: 1
top level: BBa_K1399010
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383942/range2383942/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383942/range2383942/1
type: Range
persistent identity: range2383942
display id: range2383942
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 227
end: 229
orientation: inline

BBa_K283033
type: ComponentDefinition
persistent identity: BBa K283033
display id: BBa_K283033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter-rbs-CI (lambda LVA)
creator: Shi Lei, Nelson Chu, W Ling
created: 2009-10-15T11:00:00Z
modified: 2015-05-08T01:11:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _383_
m user id: 0
owner id: 5494
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part generates chez protein under a hypoxia inducible promoter panas. The cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement and is tagged by a 6-Histidine tail.
mutable notes: BBa_K283033
mutable provenance: The rbs, double terminator comes from the registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23100
component: BBa_C0051
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_J23100
sequence annotation: BBa_C0051
sequence: https://synbiohub.org/public/igem/BBa_K283033_sequence/1

BBa_J23100
type: Component
persistent identity: component2246767
display id: component2246767
version: 1
top level: BBa_K283033
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_C0051
type: Component
persistent identity: component2246773
display id: component2246773
version: 1
top level: BBa_K283033
access: public
definition: https://synbiohub.org/public/igem/BBa_C0051/1

BBa_B0034
type: Component
persistent identity: component2246769
display id: component2246769
version: 1
top level: BBa_K283033
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2246769
display id: annotation2246769
version: 1
top level: BBa_K283033
location: https://synbiohub.org/public/igem/BBa_K283033/annotation2246769/range2246769/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K283033/annotation2246769/range2246769/1
type: Range
persistent identity: range2246769
display id: range2246769
version: 1
direction: direction/reverse
top level: BBa_K283033
start: 44
end: 55
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2246767
display id: annotation2246767
version: 1
top level: BBa_K283033
location: https://synbiohub.org/public/igem/BBa_K283033/annotation2246767/range2246767/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K283033/annotation2246767/range2246767/1
type: Range
persistent identity: range2246767
display id: range2246767
version: 1
direction: direction/reverse
top level: BBa_K283033
start: 1
end: 35
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation2246773
display id: annotation2246773
version: 1
top level: BBa_K283033
location: https://synbiohub.org/public/igem/BBa_K283033/annotation2246773/range2246773/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_K283033/annotation2246773/range2246773/1
type: Range
persistent identity: range2246773
display id: range2246773
version: 1
direction: direction/reverse
top level: BBa_K283033
start: 62
end: 836
orientation: inline

BBa_I731003
type: ComponentDefinition
persistent identity: BBa I731003
display id: BBa_I731003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: protein generator (EXAMPLE ONLY)
creator: Michael Strong
created: 2007-05-25T11:00:00Z
modified: 2015-08-31T04:07:55Z
discontinued: false
dominant: false
group u list: _127_
m user id: 0
owner id: 1894
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: protein generator (EXAMPLE ONLY), version of fimA promoter, strong ribosome binding site, and strong terminator
mutable notes: protein generator (EXAMPLE ONLY)
mutable provenance: BBa_J23100 Promoter BBa_B0030 RBS BBa_I731002 FimA BBa_B0014 terminator
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0030
component: BBa_I731002
component: BBa_B0011
component: BBa_J23100
component: BBa_B0012
sequence annotation: BBa_B0030
sequence annotation: BBa_J23100
sequence annotation: BBa_I731002
sequence annotation: BBa_B0012
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_I731003_sequence/1

BBa_B0030
type: Component
persistent identity: component1933940
display id: component1933940
version: 1
top level: BBa_I731003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_I731002
type: Component
persistent identity: component1933943
display id: component1933943
version: 1
top level: BBa_I731003
access: public
definition: https://synbiohub.org/public/igem/BBa_I731002/1

BBa_B0011
type: Component
persistent identity: component1933948
display id: component1933948
version: 1
top level: BBa_I731003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0011/1

BBa_J23100
type: Component
persistent identity: component1933938
display id: component1933938
version: 1
top level: BBa_I731003
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_B0012
type: Component
persistent identity: component1933944
display id: component1933944
version: 1
top level: BBa_I731003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1933940
display id: annotation1933940
version: 1
top level: BBa_I731003
location: https://synbiohub.org/public/igem/BBa_I731003/annotation1933940/range1933940/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_I731003/annotation1933940/range1933940/1
type: Range
persistent identity: range1933940
display id: range1933940
version: 1
direction: direction/forward
top level: BBa_I731003
start: 44
end: 58
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation1933938
display id: annotation1933938
version: 1
top level: BBa_I731003
location: https://synbiohub.org/public/igem/BBa_I731003/annotation1933938/range1933938/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_I731003/annotation1933938/range1933938/1
type: Range
persistent identity: range1933938
display id: range1933938
version: 1
direction: direction/reverse
top level: BBa_I731003
start: 1
end: 35
orientation: inline

BBa_I731002
type: SequenceAnnotation
persistent identity: annotation1933943
display id: annotation1933943
version: 1
top level: BBa_I731003
location: https://synbiohub.org/public/igem/BBa_I731003/annotation1933943/range1933943/1
component: BBa_I731002

https://synbiohub.org/public/igem/BBa_I731003/annotation1933943/range1933943/1
type: Range
persistent identity: range1933943
display id: range1933943
version: 1
direction: direction/reverse
top level: BBa_I731003
start: 65
end: 670
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1933944
display id: annotation1933944
version: 1
top level: BBa_I731003
location: https://synbiohub.org/public/igem/BBa_I731003/annotation1933944/range1933944/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I731003/annotation1933944/range1933944/1
type: Range
persistent identity: range1933944
display id: range1933944
version: 1
direction: direction/forward
top level: BBa_I731003
start: 679
end: 719
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation1933948
display id: annotation1933948
version: 1
top level: BBa_I731003
location: https://synbiohub.org/public/igem/BBa_I731003/annotation1933948/range1933948/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_I731003/annotation1933948/range1933948/1
type: Range
persistent identity: range1933948
display id: range1933948
version: 1
direction: direction/forward
top level: BBa_I731003
start: 728
end: 773
orientation: inline

WOG
type: ComponentDefinition
persistent identity: BBa J45120
display id: BBa_J45120
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Wintergreen odor generator
creator: MIT IGEM 2006
created: 2006-08-09T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 1147
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is the wintergreen generating device hooked up to a constitutive promoter with a different RBS (B0032, different from 45100).
mutable notes: N/A
mutable provenance: Built off biobricks R0040 and 45119.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0010
component: BBa_B0012
component: BBa_R0040
component: BBa_B0032
component: BBa_J45004
sequence annotation: BBa_B0010
sequence annotation: BBa_R0040
sequence annotation: BBa_J45004
sequence annotation: BBa_B0012
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_J45120_sequence/1

BBa_B0010
type: Component
persistent identity: component1894777
display id: component1894777
version: 1
top level: WOG
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1894779
display id: component1894779
version: 1
top level: WOG
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0040
type: Component
persistent identity: component1894766
display id: component1894766
version: 1
top level: WOG
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0032
type: Component
persistent identity: component1894771
display id: component1894771
version: 1
top level: WOG
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_J45004
type: Component
persistent identity: component1894776
display id: component1894776
version: 1
top level: WOG
access: public
definition: https://synbiohub.org/public/igem/BBa_J45004/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1894777
display id: annotation1894777
version: 1
top level: WOG
location: https://synbiohub.org/public/igem/BBa_J45120/annotation1894777/range1894777/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J45120/annotation1894777/range1894777/1
type: Range
persistent identity: range1894777
display id: range1894777
version: 1
direction: direction/forward
top level: WOG
start: 1164
end: 1243
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1894766
display id: annotation1894766
version: 1
top level: WOG
location: https://synbiohub.org/public/igem/BBa_J45120/annotation1894766/range1894766/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_J45120/annotation1894766/range1894766/1
type: Range
persistent identity: range1894766
display id: range1894766
version: 1
direction: direction/forward
top level: WOG
start: 1
end: 54
orientation: inline

BBa_J45004
type: SequenceAnnotation
persistent identity: annotation1894776
display id: annotation1894776
version: 1
top level: WOG
location: https://synbiohub.org/public/igem/BBa_J45120/annotation1894776/range1894776/1
component: BBa_J45004

https://synbiohub.org/public/igem/BBa_J45120/annotation1894776/range1894776/1
type: Range
persistent identity: range1894776
display id: range1894776
version: 1
direction: direction/reverse
top level: WOG
start: 82
end: 1155
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1894779
display id: annotation1894779
version: 1
top level: WOG
location: https://synbiohub.org/public/igem/BBa_J45120/annotation1894779/range1894779/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J45120/annotation1894779/range1894779/1
type: Range
persistent identity: range1894779
display id: range1894779
version: 1
direction: direction/forward
top level: WOG
start: 1252
end: 1292
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1894771
display id: annotation1894771
version: 1
top level: WOG
location: https://synbiohub.org/public/igem/BBa_J45120/annotation1894771/range1894771/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_J45120/annotation1894771/range1894771/1
type: Range
persistent identity: range1894771
display id: range1894771
version: 1
direction: direction/forward
top level: WOG
start: 63
end: 75
orientation: inline

BBa_I760001
type: ComponentDefinition
persistent identity: BBa I760001
display id: BBa_I760001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ech protein generator for vanillin biosynthesis
creator: Alexej Skvortsov
created: 2007-06-26T11:00:00Z
modified: 2015-08-31T04:08:08Z
discontinued: false
dominant: true
group u list: _148_
m user id: 0
owner id: 1863
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a part, that helps to synthesize vanillin from ferulic acid with the help of fcs
mutable notes: Needs to be used in conjuction with fcs in order to generate vanillin
mutable provenance: uses <partinfo>BBa_I760000</partinfo>, promotor, RBS and terminator. Promotor is EPTG inducible. Contains high efficiency RBS and terminator.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0011
component: BBa_R0011
component: BBa_B0012
component: BBa_I760000
component: BBa_B0034
sequence annotation: BBa_R0011
sequence annotation: BBa_I760000
sequence annotation: BBa_B0011
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I760001_sequence/1

BBa_B0011
type: Component
persistent identity: component1935725
display id: component1935725
version: 1
top level: BBa_I760001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0011/1

BBa_R0011
type: Component
persistent identity: component1935712
display id: component1935712
version: 1
top level: BBa_I760001
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_B0012
type: Component
persistent identity: component1935721
display id: component1935721
version: 1
top level: BBa_I760001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_I760000
type: Component
persistent identity: component1935720
display id: component1935720
version: 1
top level: BBa_I760001
access: public
definition: https://synbiohub.org/public/igem/BBa_I760000/1

BBa_B0034
type: Component
persistent identity: component1935718
display id: component1935718
version: 1
top level: BBa_I760001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1935712
display id: annotation1935712
version: 1
top level: BBa_I760001
location: https://synbiohub.org/public/igem/BBa_I760001/annotation1935712/range1935712/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_I760001/annotation1935712/range1935712/1
type: Range
persistent identity: range1935712
display id: range1935712
version: 1
direction: direction/forward
top level: BBa_I760001
start: 1
end: 54
orientation: inline

BBa_I760000
type: SequenceAnnotation
persistent identity: annotation1935720
display id: annotation1935720
version: 1
top level: BBa_I760001
location: https://synbiohub.org/public/igem/BBa_I760001/annotation1935720/range1935720/1
component: BBa_I760000

https://synbiohub.org/public/igem/BBa_I760001/annotation1935720/range1935720/1
type: Range
persistent identity: range1935720
display id: range1935720
version: 1
direction: direction/reverse
top level: BBa_I760001
start: 82
end: 912
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation1935725
display id: annotation1935725
version: 1
top level: BBa_I760001
location: https://synbiohub.org/public/igem/BBa_I760001/annotation1935725/range1935725/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_I760001/annotation1935725/range1935725/1
type: Range
persistent identity: range1935725
display id: range1935725
version: 1
direction: direction/forward
top level: BBa_I760001
start: 970
end: 1015
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1935721
display id: annotation1935721
version: 1
top level: BBa_I760001
location: https://synbiohub.org/public/igem/BBa_I760001/annotation1935721/range1935721/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I760001/annotation1935721/range1935721/1
type: Range
persistent identity: range1935721
display id: range1935721
version: 1
direction: direction/forward
top level: BBa_I760001
start: 921
end: 961
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1935718
display id: annotation1935718
version: 1
top level: BBa_I760001
location: https://synbiohub.org/public/igem/BBa_I760001/annotation1935718/range1935718/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I760001/annotation1935718/range1935718/1
type: Range
persistent identity: range1935718
display id: range1935718
version: 1
direction: direction/reverse
top level: BBa_I760001
start: 64
end: 75
orientation: inline

BBa_I769106
type: ComponentDefinition
persistent identity: BBa I769106
display id: BBa_I769106
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ech protein generator for vanillin biosynthesis
creator: Meagan Lizarazo
created: 2007-06-26T11:00:00Z
modified: 2015-08-31T04:08:10Z
discontinued: false
dominant: true
experience: experience/None
group u list: _162_
m user id: 0
owner id: 457
owning group id: 67
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This part helps to synthesize vanillin from ferulic acid with the help of fcs.
mutable notes: Needs to be used in conjunction with fcs in order to generate vanillin.
mutable provenance: Uses <partinfo>BBa_I769105</partinfo> as well as a promoter, RBS, and terminator. Uses IPTG inducible promoter in order to test construct. Contains high efficiency RBS and terminator.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0012
component: BBa_B0011
component: BBa_B0034
component: BBa_I769104
component: BBa_R0011
sequence annotation: BBa_R0011
sequence annotation: BBa_B0011
sequence annotation: BBa_I769104
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I769106_sequence/1

BBa_B0012
type: Component
persistent identity: component1935832
display id: component1935832
version: 1
top level: BBa_I769106
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0011
type: Component
persistent identity: component1935836
display id: component1935836
version: 1
top level: BBa_I769106
access: public
definition: https://synbiohub.org/public/igem/BBa_B0011/1

BBa_B0034
type: Component
persistent identity: component1935829
display id: component1935829
version: 1
top level: BBa_I769106
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I769104
type: Component
persistent identity: component1935831
display id: component1935831
version: 1
top level: BBa_I769106
access: public
definition: https://synbiohub.org/public/igem/BBa_I769104/1

BBa_R0011
type: Component
persistent identity: component1935823
display id: component1935823
version: 1
top level: BBa_I769106
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1935823
display id: annotation1935823
version: 1
top level: BBa_I769106
location: https://synbiohub.org/public/igem/BBa_I769106/annotation1935823/range1935823/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_I769106/annotation1935823/range1935823/1
type: Range
persistent identity: range1935823
display id: range1935823
version: 1
direction: direction/forward
top level: BBa_I769106
start: 1
end: 54
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation1935836
display id: annotation1935836
version: 1
top level: BBa_I769106
location: https://synbiohub.org/public/igem/BBa_I769106/annotation1935836/range1935836/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_I769106/annotation1935836/range1935836/1
type: Range
persistent identity: range1935836
display id: range1935836
version: 1
direction: direction/forward
top level: BBa_I769106
start: 970
end: 1015
orientation: inline

BBa_I769104
type: SequenceAnnotation
persistent identity: annotation1935831
display id: annotation1935831
version: 1
top level: BBa_I769106
location: https://synbiohub.org/public/igem/BBa_I769106/annotation1935831/range1935831/1
component: BBa_I769104

https://synbiohub.org/public/igem/BBa_I769106/annotation1935831/range1935831/1
type: Range
persistent identity: range1935831
display id: range1935831
version: 1
direction: direction/reverse
top level: BBa_I769106
start: 82
end: 912
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1935829
display id: annotation1935829
version: 1
top level: BBa_I769106
location: https://synbiohub.org/public/igem/BBa_I769106/annotation1935829/range1935829/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I769106/annotation1935829/range1935829/1
type: Range
persistent identity: range1935829
display id: range1935829
version: 1
direction: direction/reverse
top level: BBa_I769106
start: 64
end: 75
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1935832
display id: annotation1935832
version: 1
top level: BBa_I769106
location: https://synbiohub.org/public/igem/BBa_I769106/annotation1935832/range1935832/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I769106/annotation1935832/range1935832/1
type: Range
persistent identity: range1935832
display id: range1935832
version: 1
direction: direction/forward
top level: BBa_I769106
start: 921
end: 961
orientation: inline

BBa_J45270
type: ComponentDefinition
persistent identity: BBa J45270
display id: BBa_J45270
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Stationary-phase-dependent banana odor generator
creator: Andr?? Green II
created: 2006-10-27T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _84_
m user id: 0
owner id: 977
owning group id: 84
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is an ATF1-1148 protein generator under control by the osmY stationary phase promoter (BBa_J45993). It is almost identical to J45250, except that: 1. The promoter is the truncated version of osmY and 2. it uses the B0030 RBS instead of B0032 (which is in J45250).
mutable notes: none
mutable provenance: coming
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0010
component: BBa_J45014
component: BBa_J45993
component: BBa_B0012
component: BBa_B0032
sequence annotation: BBa_B0012
sequence annotation: BBa_J45993
sequence annotation: BBa_B0032
sequence annotation: BBa_J45014
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_J45270_sequence/1

BBa_B0010
type: Component
persistent identity: component1908098
display id: component1908098
version: 1
top level: BBa_J45270
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_J45014
type: Component
persistent identity: component1908097
display id: component1908097
version: 1
top level: BBa_J45270
access: public
definition: https://synbiohub.org/public/igem/BBa_J45014/1

BBa_J45993
type: Component
persistent identity: component1908089
display id: component1908089
version: 1
top level: BBa_J45270
access: public
definition: https://synbiohub.org/public/igem/BBa_J45993/1

BBa_B0012
type: Component
persistent identity: component1908100
display id: component1908100
version: 1
top level: BBa_J45270
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0032
type: Component
persistent identity: component1908091
display id: component1908091
version: 1
top level: BBa_J45270
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1908100
display id: annotation1908100
version: 1
top level: BBa_J45270
location: https://synbiohub.org/public/igem/BBa_J45270/annotation1908100/range1908100/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J45270/annotation1908100/range1908100/1
type: Range
persistent identity: range1908100
display id: range1908100
version: 1
direction: direction/forward
top level: BBa_J45270
start: 1762
end: 1802
orientation: inline

BBa_J45993
type: SequenceAnnotation
persistent identity: annotation1908089
display id: annotation1908089
version: 1
top level: BBa_J45270
location: https://synbiohub.org/public/igem/BBa_J45270/annotation1908089/range1908089/1
component: BBa_J45993

https://synbiohub.org/public/igem/BBa_J45270/annotation1908089/range1908089/1
type: Range
persistent identity: range1908089
display id: range1908089
version: 1
direction: direction/reverse
top level: BBa_J45270
start: 1
end: 57
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1908091
display id: annotation1908091
version: 1
top level: BBa_J45270
location: https://synbiohub.org/public/igem/BBa_J45270/annotation1908091/range1908091/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_J45270/annotation1908091/range1908091/1
type: Range
persistent identity: range1908091
display id: range1908091
version: 1
direction: direction/forward
top level: BBa_J45270
start: 66
end: 78
orientation: inline

BBa_J45014
type: SequenceAnnotation
persistent identity: annotation1908097
display id: annotation1908097
version: 1
top level: BBa_J45270
location: https://synbiohub.org/public/igem/BBa_J45270/annotation1908097/range1908097/1
component: BBa_J45014

https://synbiohub.org/public/igem/BBa_J45270/annotation1908097/range1908097/1
type: Range
persistent identity: range1908097
display id: range1908097
version: 1
direction: direction/reverse
top level: BBa_J45270
start: 85
end: 1665
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1908098
display id: annotation1908098
version: 1
top level: BBa_J45270
location: https://synbiohub.org/public/igem/BBa_J45270/annotation1908098/range1908098/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J45270/annotation1908098/range1908098/1
type: Range
persistent identity: range1908098
display id: range1908098
version: 1
direction: direction/forward
top level: BBa_J45270
start: 1674
end: 1753
orientation: inline

BBa_I738001
type: ComponentDefinition
persistent identity: BBa I738001
display id: BBa_I738001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: generates the feruloyl-CoA hydratase for vanillin
creator: Kari Clase
created: 2007-05-25T11:00:00Z
modified: 2015-08-31T04:08:01Z
discontinued: false
dominant: false
group u list: _146_
m user id: 0
owner id: 1076
owning group id: 54
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Protein generator version of BBa_1738002 Promoter, strong ribosome binding site, and strong terminator.
mutable notes: Strong ribosome binding site, well documented promoter, strong terminator
mutable provenance: sdd Biobrick I738002
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_I738002
component: BBa_B0011
component: BBa_B0030
component: BBa_J23100
component: BBa_B0012
sequence annotation: BBa_I738002
sequence annotation: BBa_B0011
sequence annotation: BBa_J23100
sequence annotation: BBa_B0012
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_I738001_sequence/1

BBa_I738002
type: Component
persistent identity: component1933892
display id: component1933892
version: 1
top level: BBa_I738001
access: public
definition: https://synbiohub.org/public/igem/BBa_I738002/1

BBa_B0011
type: Component
persistent identity: component1933897
display id: component1933897
version: 1
top level: BBa_I738001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0011/1

BBa_B0030
type: Component
persistent identity: component1933889
display id: component1933889
version: 1
top level: BBa_I738001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_J23100
type: Component
persistent identity: component1933887
display id: component1933887
version: 1
top level: BBa_I738001
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_B0012
type: Component
persistent identity: component1933893
display id: component1933893
version: 1
top level: BBa_I738001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_I738002
type: SequenceAnnotation
persistent identity: annotation1933892
display id: annotation1933892
version: 1
top level: BBa_I738001
location: https://synbiohub.org/public/igem/BBa_I738001/annotation1933892/range1933892/1
component: BBa_I738002

https://synbiohub.org/public/igem/BBa_I738001/annotation1933892/range1933892/1
type: Range
persistent identity: range1933892
display id: range1933892
version: 1
direction: direction/reverse
top level: BBa_I738001
start: 65
end: 898
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation1933897
display id: annotation1933897
version: 1
top level: BBa_I738001
location: https://synbiohub.org/public/igem/BBa_I738001/annotation1933897/range1933897/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_I738001/annotation1933897/range1933897/1
type: Range
persistent identity: range1933897
display id: range1933897
version: 1
direction: direction/forward
top level: BBa_I738001
start: 956
end: 1001
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation1933887
display id: annotation1933887
version: 1
top level: BBa_I738001
location: https://synbiohub.org/public/igem/BBa_I738001/annotation1933887/range1933887/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_I738001/annotation1933887/range1933887/1
type: Range
persistent identity: range1933887
display id: range1933887
version: 1
direction: direction/reverse
top level: BBa_I738001
start: 1
end: 35
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1933893
display id: annotation1933893
version: 1
top level: BBa_I738001
location: https://synbiohub.org/public/igem/BBa_I738001/annotation1933893/range1933893/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I738001/annotation1933893/range1933893/1
type: Range
persistent identity: range1933893
display id: range1933893
version: 1
direction: direction/forward
top level: BBa_I738001
start: 907
end: 947
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1933889
display id: annotation1933889
version: 1
top level: BBa_I738001
location: https://synbiohub.org/public/igem/BBa_I738001/annotation1933889/range1933889/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_I738001/annotation1933889/range1933889/1
type: Range
persistent identity: range1933889
display id: range1933889
version: 1
direction: direction/forward
top level: BBa_I738001
start: 44
end: 58
orientation: inline

Banana
type: ComponentDefinition
persistent identity: BBa J45200
display id: BBa_J45200
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Banana odor generator
creator: Andr???? Green II
created: 2006-10-19T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 977
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Protein generator converting PoPS to the protein ATF1. ATFI encodes the enzyme alcohol acetyltransferase I (AATase I), which produces acetate esters from acetyl CoA and alcohols.
mutable notes: This part is the ATF sequence BBa_45J006 with a single point mutation at base pair 1148 (T -> C). Despite the mutation, the enzyme is still functional (kinetic studies have not been done to determine realitive functionality).
mutable provenance: This part was cloned from S. cerevisiae.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0010
component: BBa_R0040
component: BBa_J45014
component: BBa_B0030
component: BBa_B0012
sequence annotation: BBa_B0030
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_R0040
sequence annotation: BBa_J45014
sequence: https://synbiohub.org/public/igem/BBa_J45200_sequence/1

BBa_B0010
type: Component
persistent identity: component1960599
display id: component1960599
version: 1
top level: Banana
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0040
type: Component
persistent identity: component1960587
display id: component1960587
version: 1
top level: Banana
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_J45014
type: Component
persistent identity: component1960598
display id: component1960598
version: 1
top level: Banana
access: public
definition: https://synbiohub.org/public/igem/BBa_J45014/1

BBa_B0030
type: Component
persistent identity: component1960592
display id: component1960592
version: 1
top level: Banana
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_B0012
type: Component
persistent identity: component1960601
display id: component1960601
version: 1
top level: Banana
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1960592
display id: annotation1960592
version: 1
top level: Banana
location: https://synbiohub.org/public/igem/BBa_J45200/annotation1960592/range1960592/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J45200/annotation1960592/range1960592/1
type: Range
persistent identity: range1960592
display id: range1960592
version: 1
direction: direction/forward
top level: Banana
start: 63
end: 77
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1960601
display id: annotation1960601
version: 1
top level: Banana
location: https://synbiohub.org/public/igem/BBa_J45200/annotation1960601/range1960601/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J45200/annotation1960601/range1960601/1
type: Range
persistent identity: range1960601
display id: range1960601
version: 1
direction: direction/forward
top level: Banana
start: 1761
end: 1801
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1960599
display id: annotation1960599
version: 1
top level: Banana
location: https://synbiohub.org/public/igem/BBa_J45200/annotation1960599/range1960599/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J45200/annotation1960599/range1960599/1
type: Range
persistent identity: range1960599
display id: range1960599
version: 1
direction: direction/forward
top level: Banana
start: 1673
end: 1752
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1960587
display id: annotation1960587
version: 1
top level: Banana
location: https://synbiohub.org/public/igem/BBa_J45200/annotation1960587/range1960587/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_J45200/annotation1960587/range1960587/1
type: Range
persistent identity: range1960587
display id: range1960587
version: 1
direction: direction/forward
top level: Banana
start: 1
end: 54
orientation: inline

BBa_J45014
type: SequenceAnnotation
persistent identity: annotation1960598
display id: annotation1960598
version: 1
top level: Banana
location: https://synbiohub.org/public/igem/BBa_J45200/annotation1960598/range1960598/1
component: BBa_J45014

https://synbiohub.org/public/igem/BBa_J45200/annotation1960598/range1960598/1
type: Range
persistent identity: range1960598
display id: range1960598
version: 1
direction: direction/reverse
top level: Banana
start: 84
end: 1664
orientation: inline

BBa_I711021
type: ComponentDefinition
persistent identity: BBa I711021
display id: BBa_I711021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Butanol dehydrogenase generator, beta-butryaldehyde -> butanol
creator: Ranjan Khan
created: 2007-07-15T11:00:00Z
modified: 2015-08-31T04:07:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _158_
m user id: 0
owner id: 1498
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part constitutively translates the aad gene, which produces and alcohol dehydrogenase that may be important in the synthesis of butanol from beta-butryaldehyde.
mutable notes: None.
mutable provenance: Clostridium acetobutylicum ATCC 824
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0014
component: BBa_B0030
component: BBa_I711010
component: BBa_J23119
sequence annotation: BBa_B0014
sequence annotation: BBa_J23119
sequence annotation: BBa_I711010
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_I711021_sequence/1

BBa_B0014
type: Component
persistent identity: component2222033
display id: component2222033
version: 1
top level: BBa_I711021
access: public
definition: https://synbiohub.org/public/igem/BBa_B0014/1

BBa_B0030
type: Component
persistent identity: component2222024
display id: component2222024
version: 1
top level: BBa_I711021
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_I711010
type: Component
persistent identity: component2222026
display id: component2222026
version: 1
top level: BBa_I711021
access: public
definition: https://synbiohub.org/public/igem/BBa_I711010/1

BBa_J23119
type: Component
persistent identity: component2222022
display id: component2222022
version: 1
top level: BBa_I711021
access: public
definition: https://synbiohub.org/public/igem/BBa_J23119/1

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2222033
display id: annotation2222033
version: 1
top level: BBa_I711021
location: https://synbiohub.org/public/igem/BBa_I711021/annotation2222033/range2222033/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_I711021/annotation2222033/range2222033/1
type: Range
persistent identity: range2222033
display id: range2222033
version: 1
direction: direction/reverse
top level: BBa_I711021
start: 1261
end: 1355
orientation: inline

BBa_J23119
type: SequenceAnnotation
persistent identity: annotation2222022
display id: annotation2222022
version: 1
top level: BBa_I711021
location: https://synbiohub.org/public/igem/BBa_I711021/annotation2222022/range2222022/1
component: BBa_J23119

https://synbiohub.org/public/igem/BBa_I711021/annotation2222022/range2222022/1
type: Range
persistent identity: range2222022
display id: range2222022
version: 1
direction: direction/reverse
top level: BBa_I711021
start: 1
end: 35
orientation: inline

BBa_I711010
type: SequenceAnnotation
persistent identity: annotation2222026
display id: annotation2222026
version: 1
top level: BBa_I711021
location: https://synbiohub.org/public/igem/BBa_I711021/annotation2222026/range2222026/1
component: BBa_I711010

https://synbiohub.org/public/igem/BBa_I711021/annotation2222026/range2222026/1
type: Range
persistent identity: range2222026
display id: range2222026
version: 1
direction: direction/reverse
top level: BBa_I711021
start: 65
end: 1252
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2222024
display id: annotation2222024
version: 1
top level: BBa_I711021
location: https://synbiohub.org/public/igem/BBa_I711021/annotation2222024/range2222024/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_I711021/annotation2222024/range2222024/1
type: Range
persistent identity: range2222024
display id: range2222024
version: 1
direction: direction/forward
top level: BBa_I711021
start: 44
end: 58
orientation: inline

BBa_I5020
type: ComponentDefinition
persistent identity: BBa I5020
display id: BBa_I5020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Olmecs: Inducible expression of 434 cI repressor
creator: Olmecs 2003 IAP Team
created: 2003-11-27T12:00:00Z
modified: 2015-08-31T04:07:42Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_C0052
component: BBa_B0012
component: BBa_B0011
component: BBa_B0034
component: BBa_R0011
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_C0052
sequence annotation: BBa_R0011
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_I5020_sequence/1

BBa_C0052
type: Component
persistent identity: component2221106
display id: component2221106
version: 1
top level: BBa_I5020
access: public
definition: https://synbiohub.org/public/igem/BBa_C0052/1

BBa_B0012
type: Component
persistent identity: component2221109
display id: component2221109
version: 1
top level: BBa_I5020
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0011
type: Component
persistent identity: component2221113
display id: component2221113
version: 1
top level: BBa_I5020
access: public
definition: https://synbiohub.org/public/igem/BBa_B0011/1

BBa_B0034
type: Component
persistent identity: component2221104
display id: component2221104
version: 1
top level: BBa_I5020
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0011
type: Component
persistent identity: component2221098
display id: component2221098
version: 1
top level: BBa_I5020
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2221104
display id: annotation2221104
version: 1
top level: BBa_I5020
location: https://synbiohub.org/public/igem/BBa_I5020/annotation2221104/range2221104/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I5020/annotation2221104/range2221104/1
type: Range
persistent identity: range2221104
display id: range2221104
version: 1
direction: direction/reverse
top level: BBa_I5020
start: 64
end: 75
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2221109
display id: annotation2221109
version: 1
top level: BBa_I5020
location: https://synbiohub.org/public/igem/BBa_I5020/annotation2221109/range2221109/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I5020/annotation2221109/range2221109/1
type: Range
persistent identity: range2221109
display id: range2221109
version: 1
direction: direction/forward
top level: BBa_I5020
start: 784
end: 824
orientation: inline

BBa_C0052
type: SequenceAnnotation
persistent identity: annotation2221106
display id: annotation2221106
version: 1
top level: BBa_I5020
location: https://synbiohub.org/public/igem/BBa_I5020/annotation2221106/range2221106/1
component: BBa_C0052

https://synbiohub.org/public/igem/BBa_I5020/annotation2221106/range2221106/1
type: Range
persistent identity: range2221106
display id: range2221106
version: 1
direction: direction/forward
top level: BBa_I5020
start: 82
end: 750
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2221098
display id: annotation2221098
version: 1
top level: BBa_I5020
location: https://synbiohub.org/public/igem/BBa_I5020/annotation2221098/range2221098/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_I5020/annotation2221098/range2221098/1
type: Range
persistent identity: range2221098
display id: range2221098
version: 1
direction: direction/forward
top level: BBa_I5020
start: 1
end: 54
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2221113
display id: annotation2221113
version: 1
top level: BBa_I5020
location: https://synbiohub.org/public/igem/BBa_I5020/annotation2221113/range2221113/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_I5020/annotation2221113/range2221113/1
type: Range
persistent identity: range2221113
display id: range2221113
version: 1
direction: direction/forward
top level: BBa_I5020
start: 833
end: 878
orientation: inline

BBa_I711020
type: ComponentDefinition
persistent identity: BBa I711020
display id: BBa_I711020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cellulase Generator
creator: Ranjan Khan
created: 2007-07-15T11:00:00Z
modified: 2015-08-31T04:07:45Z
discontinued: true
dominant: false
experience: experience/None
group u list: _158_
m user id: 0
owner id: 1498
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: This part constitutively translates the cel5F gene from Saccharophagus degradans 2-40. The cellulase produced help degrade cellulose into glucose.
mutable notes: None
mutable provenance: Saccharophagus degradans 2-40
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0030
component: BBa_J23119
component: BBa_I711013
component: BBa_B0011
component: BBa_B0012
sequence annotation: BBa_I711013
sequence annotation: BBa_B0012
sequence annotation: BBa_B0030
sequence annotation: BBa_B0011
sequence annotation: BBa_J23119
sequence: https://synbiohub.org/public/igem/BBa_I711020_sequence/1

BBa_B0030
type: Component
persistent identity: component1938385
display id: component1938385
version: 1
top level: BBa_I711020
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_J23119
type: Component
persistent identity: component1938383
display id: component1938383
version: 1
top level: BBa_I711020
access: public
definition: https://synbiohub.org/public/igem/BBa_J23119/1

BBa_I711013
type: Component
persistent identity: component1938387
display id: component1938387
version: 1
top level: BBa_I711020
access: public
definition: https://synbiohub.org/public/igem/BBa_I711013/1

BBa_B0011
type: Component
persistent identity: component1938392
display id: component1938392
version: 1
top level: BBa_I711020
access: public
definition: https://synbiohub.org/public/igem/BBa_B0011/1

BBa_B0012
type: Component
persistent identity: component1938388
display id: component1938388
version: 1
top level: BBa_I711020
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_I711013
type: SequenceAnnotation
persistent identity: annotation1938387
display id: annotation1938387
version: 1
top level: BBa_I711020
location: https://synbiohub.org/public/igem/BBa_I711020/annotation1938387/range1938387/1
component: BBa_I711013

https://synbiohub.org/public/igem/BBa_I711020/annotation1938387/range1938387/1
type: Range
persistent identity: range1938387
display id: range1938387
version: 1
direction: direction/reverse
top level: BBa_I711020
start: 67
end: 1164
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1938388
display id: annotation1938388
version: 1
top level: BBa_I711020
location: https://synbiohub.org/public/igem/BBa_I711020/annotation1938388/range1938388/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I711020/annotation1938388/range1938388/1
type: Range
persistent identity: range1938388
display id: range1938388
version: 1
direction: direction/forward
top level: BBa_I711020
start: 1173
end: 1213
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1938385
display id: annotation1938385
version: 1
top level: BBa_I711020
location: https://synbiohub.org/public/igem/BBa_I711020/annotation1938385/range1938385/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_I711020/annotation1938385/range1938385/1
type: Range
persistent identity: range1938385
display id: range1938385
version: 1
direction: direction/forward
top level: BBa_I711020
start: 44
end: 58
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation1938392
display id: annotation1938392
version: 1
top level: BBa_I711020
location: https://synbiohub.org/public/igem/BBa_I711020/annotation1938392/range1938392/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_I711020/annotation1938392/range1938392/1
type: Range
persistent identity: range1938392
display id: range1938392
version: 1
direction: direction/forward
top level: BBa_I711020
start: 1222
end: 1267
orientation: inline

BBa_J23119
type: SequenceAnnotation
persistent identity: annotation1938383
display id: annotation1938383
version: 1
top level: BBa_I711020
location: https://synbiohub.org/public/igem/BBa_I711020/annotation1938383/range1938383/1
component: BBa_J23119

https://synbiohub.org/public/igem/BBa_I711020/annotation1938383/range1938383/1
type: Range
persistent identity: range1938383
display id: range1938383
version: 1
direction: direction/reverse
top level: BBa_I711020
start: 1
end: 35
orientation: inline

BBa_K1399012
type: ComponentDefinition
persistent identity: BBa K1399012
display id: BBa_K1399012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac-RFP(LAA)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Lactose/IPTG inducible promoter with RBS, RFP reporter tagged with LAA-ssrA degradation tag and terminator. The tagged RFP is actively degraded within cell, thus provides better temporal resolution of red fluorescence and promoter activity.
mutable notes: Part was assembled using BrickClip assembly (BBF RFC 104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
mutable provenance: other biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: T7 TE
sequence annotation: stem_loop
sequence annotation: barcode from BBa_E1010
sequence annotation: BBa_B0015
sequence annotation: LacI binding site
sequence annotation: BBa_B0010
sequence annotation: BBa_R0010
sequence annotation: -10
sequence annotation: BBa_K1399002
sequence annotation: stop
sequence annotation: CAP binding site
sequence annotation: start
sequence annotation: tag
sequence annotation: conserved
sequence annotation: BBa_B0034
sequence annotation: -35
sequence annotation: RFP
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_B0012
sequence annotation: polya
sequence: https://synbiohub.org/public/igem/BBa_K1399012_sequence/1

T7 TE
type: SequenceAnnotation
persistent identity: annotation2384004
display id: annotation2384004
version: 1
top level: BBa_K1399012
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2384004/range2384004/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2384004/range2384004/1
type: Range
persistent identity: range2384004
display id: range2384004
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 1069
end: 1088
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2384002
display id: annotation2384002
version: 1
top level: BBa_K1399012
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2384002/range2384002/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2384002/range2384002/1
type: Range
persistent identity: range2384002
display id: range2384002
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 985
end: 1028
orientation: inline

barcode from BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2386312
display id: annotation2386312
version: 1
top level: BBa_K1399012
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2386312/range2386312/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2386312/range2386312/1
type: Range
persistent identity: range2386312
display id: range2386312
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 941
end: 965
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2384007
display id: annotation2384007
version: 1
top level: BBa_K1399012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2384007/range2384007/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2384007/range2384007/1
type: Range
persistent identity: range2384007
display id: range2384007
version: 1
direction: direction/reverse
top level: BBa_K1399012
start: 974
end: 1102
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation2383991
display id: annotation2383991
version: 1
top level: BBa_K1399012
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383991/range2383991/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383991/range2383991/1
type: Range
persistent identity: range2383991
display id: range2383991
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 166
end: 200
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2384001
display id: annotation2384001
version: 1
top level: BBa_K1399012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2384001/range2384001/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2384001/range2384001/1
type: Range
persistent identity: range2384001
display id: range2384001
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 974
end: 1053
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2383986
display id: annotation2383986
version: 1
top level: BBa_K1399012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383986/range2383986/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383986/range2383986/1
type: Range
persistent identity: range2383986
display id: range2383986
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 1
end: 200
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2383990
display id: annotation2383990
version: 1
top level: BBa_K1399012
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383990/range2383990/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383990/range2383990/1
type: Range
persistent identity: range2383990
display id: range2383990
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 161
end: 166
orientation: inline

BBa_K1399002
type: SequenceAnnotation
persistent identity: annotation2384000
display id: annotation2384000
version: 1
top level: BBa_K1399012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2384000/range2384000/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2384000/range2384000/1
type: Range
persistent identity: range2384000
display id: range2384000
version: 1
direction: direction/reverse
top level: BBa_K1399012
start: 227
end: 940
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383999
display id: annotation2383999
version: 1
top level: BBa_K1399012
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383999/range2383999/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383999/range2383999/1
type: Range
persistent identity: range2383999
display id: range2383999
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 938
end: 940
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation2383988
display id: annotation2383988
version: 1
top level: BBa_K1399012
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383988/range2383988/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383988/range2383988/1
type: Range
persistent identity: range2383988
display id: range2383988
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 89
end: 126
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2383992
display id: annotation2383992
version: 1
top level: BBa_K1399012
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383992/range2383992/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383992/range2383992/1
type: Range
persistent identity: range2383992
display id: range2383992
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 173
end: 173
orientation: inline

tag
type: SequenceAnnotation
persistent identity: annotation2383997
display id: annotation2383997
version: 1
top level: BBa_K1399012
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383997/range2383997/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383997/range2383997/1
type: Range
persistent identity: range2383997
display id: range2383997
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 902
end: 934
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2383993
display id: annotation2383993
version: 1
top level: BBa_K1399012
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383993/range2383993/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383993/range2383993/1
type: Range
persistent identity: range2383993
display id: range2383993
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 213
end: 216
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2383994
display id: annotation2383994
version: 1
top level: BBa_K1399012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383994/range2383994/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383994/range2383994/1
type: Range
persistent identity: range2383994
display id: range2383994
version: 1
direction: direction/reverse
top level: BBa_K1399012
start: 209
end: 220
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2383989
display id: annotation2383989
version: 1
top level: BBa_K1399012
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383989/range2383989/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383989/range2383989/1
type: Range
persistent identity: range2383989
display id: range2383989
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 137
end: 142
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2383995
display id: annotation2383995
version: 1
top level: BBa_K1399012
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383995/range2383995/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383995/range2383995/1
type: Range
persistent identity: range2383995
display id: range2383995
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 227
end: 229
orientation: inline

RFP
type: SequenceAnnotation
persistent identity: annotation2383996
display id: annotation2383996
version: 1
top level: BBa_K1399012
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383996/range2383996/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383996/range2383996/1
type: Range
persistent identity: range2383996
display id: range2383996
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 230
end: 901
orientation: inline

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation2383987
display id: annotation2383987
version: 1
top level: BBa_K1399012
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383987/range2383987/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383987/range2383987/1
type: Range
persistent identity: range2383987
display id: range2383987
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 1
end: 88
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2384003
display id: annotation2384003
version: 1
top level: BBa_K1399012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2384003/range2384003/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2384003/range2384003/1
type: Range
persistent identity: range2384003
display id: range2384003
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 1062
end: 1102
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2384006
display id: annotation2384006
version: 1
top level: BBa_K1399012
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2384006/range2384006/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2384006/range2384006/1
type: Range
persistent identity: range2384006
display id: range2384006
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 1095
end: 1095
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383998
display id: annotation2383998
version: 1
top level: BBa_K1399012
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383998/range2383998/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383998/range2383998/1
type: Range
persistent identity: range2383998
display id: range2383998
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 935
end: 937
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2384005
display id: annotation2384005
version: 1
top level: BBa_K1399012
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2384005/range2384005/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2384005/range2384005/1
type: Range
persistent identity: range2384005
display id: range2384005
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 1089
end: 1102
orientation: inline

BBa_I718001
type: ComponentDefinition
persistent identity: BBa I718001
display id: BBa_I718001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ech My new test generator part Feruloyl CoA hydratase for vanilin
creator: Samuel Bottani
created: 2007-06-26T11:00:00Z
is replaced by: BBa_I760001
modified: 2015-08-31T04:07:52Z
discontinued: true
dominant: false
group u list: _141_
m user id: 0
owner id: 1613
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: This is the part I am editing in the teaching session. It helps synthesize vanilin from ferulic acid with the help of fcs.
mutable notes: Needs to be used in conjunction with fcs in order to generate vanilin. It uses an ITPG promoter and an high efficiency RBS terminator.
mutable provenance: Uses <partinfo>BBa_I71800</partinfo> as well as a promoter, RBS, and terminator.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_I718000
component: BBa_B0034
component: BBa_B0011
component: BBa_R0011
component: BBa_B0012
sequence annotation: BBa_I718000
sequence annotation: BBa_R0011
sequence annotation: BBa_B0034
sequence annotation: BBa_B0011
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I718001_sequence/1

BBa_I718000
type: Component
persistent identity: component1935816
display id: component1935816
version: 1
top level: BBa_I718001
access: public
definition: https://synbiohub.org/public/igem/BBa_I718000/1

BBa_B0034
type: Component
persistent identity: component1935814
display id: component1935814
version: 1
top level: BBa_I718001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0011
type: Component
persistent identity: component1935821
display id: component1935821
version: 1
top level: BBa_I718001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0011/1

BBa_R0011
type: Component
persistent identity: component1935808
display id: component1935808
version: 1
top level: BBa_I718001
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_B0012
type: Component
persistent identity: component1935817
display id: component1935817
version: 1
top level: BBa_I718001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_I718000
type: SequenceAnnotation
persistent identity: annotation1935816
display id: annotation1935816
version: 1
top level: BBa_I718001
location: https://synbiohub.org/public/igem/BBa_I718001/annotation1935816/range1935816/1
component: BBa_I718000

https://synbiohub.org/public/igem/BBa_I718001/annotation1935816/range1935816/1
type: Range
persistent identity: range1935816
display id: range1935816
version: 1
direction: direction/reverse
top level: BBa_I718001
start: 82
end: 912
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1935808
display id: annotation1935808
version: 1
top level: BBa_I718001
location: https://synbiohub.org/public/igem/BBa_I718001/annotation1935808/range1935808/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_I718001/annotation1935808/range1935808/1
type: Range
persistent identity: range1935808
display id: range1935808
version: 1
direction: direction/forward
top level: BBa_I718001
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1935814
display id: annotation1935814
version: 1
top level: BBa_I718001
location: https://synbiohub.org/public/igem/BBa_I718001/annotation1935814/range1935814/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I718001/annotation1935814/range1935814/1
type: Range
persistent identity: range1935814
display id: range1935814
version: 1
direction: direction/reverse
top level: BBa_I718001
start: 64
end: 75
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation1935821
display id: annotation1935821
version: 1
top level: BBa_I718001
location: https://synbiohub.org/public/igem/BBa_I718001/annotation1935821/range1935821/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_I718001/annotation1935821/range1935821/1
type: Range
persistent identity: range1935821
display id: range1935821
version: 1
direction: direction/forward
top level: BBa_I718001
start: 970
end: 1015
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1935817
display id: annotation1935817
version: 1
top level: BBa_I718001
location: https://synbiohub.org/public/igem/BBa_I718001/annotation1935817/range1935817/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I718001/annotation1935817/range1935817/1
type: Range
persistent identity: range1935817
display id: range1935817
version: 1
direction: direction/forward
top level: BBa_I718001
start: 921
end: 961
orientation: inline

BBa_K1399013
type: ComponentDefinition
persistent identity: BBa K1399013
display id: BBa_K1399013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac-RFP(DAS)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Lactose/IPTG inducible promoter with RFP reporter tagged with DAS-ssrA degradation tag followed by terminator. The tagged RFP is degraded within cell, thus provides better temporal resolution of red fluorescence and promoter activity. Presence of SspB is crucial for effective protein degradation.
mutable notes: Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
mutable provenance: other biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: BBa_R0010
sequence annotation: stop
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: end of LacI coding region (inactive)
sequence annotation: RFP
sequence annotation: barcode from BBa_E1010
sequence annotation: start
sequence annotation: CAP binding site
sequence annotation: polya
sequence annotation: -35
sequence annotation: BBa_B0015
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: conserved
sequence annotation: BBa_B0034
sequence annotation: BBa_K1399003
sequence annotation: DAS-ssrA tag
sequence annotation: stem_loop
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_K1399013_sequence/1

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2384012
display id: annotation2384012
version: 1
top level: BBa_K1399013
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384012/range2384012/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384012/range2384012/1
type: Range
persistent identity: range2384012
display id: range2384012
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 1
end: 200
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2384024
display id: annotation2384024
version: 1
top level: BBa_K1399013
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384024/range2384024/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384024/range2384024/1
type: Range
persistent identity: range2384024
display id: range2384024
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 935
end: 937
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2384029
display id: annotation2384029
version: 1
top level: BBa_K1399013
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384029/range2384029/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384029/range2384029/1
type: Range
persistent identity: range2384029
display id: range2384029
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 1062
end: 1102
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2384030
display id: annotation2384030
version: 1
top level: BBa_K1399013
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384030/range2384030/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384030/range2384030/1
type: Range
persistent identity: range2384030
display id: range2384030
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 1069
end: 1088
orientation: inline

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation2384013
display id: annotation2384013
version: 1
top level: BBa_K1399013
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384013/range2384013/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384013/range2384013/1
type: Range
persistent identity: range2384013
display id: range2384013
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 1
end: 88
orientation: inline

RFP
type: SequenceAnnotation
persistent identity: annotation2384022
display id: annotation2384022
version: 1
top level: BBa_K1399013
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384022/range2384022/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384022/range2384022/1
type: Range
persistent identity: range2384022
display id: range2384022
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 230
end: 901
orientation: inline

barcode from BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2386313
display id: annotation2386313
version: 1
top level: BBa_K1399013
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2386313/range2386313/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2386313/range2386313/1
type: Range
persistent identity: range2386313
display id: range2386313
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 941
end: 965
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2384018
display id: annotation2384018
version: 1
top level: BBa_K1399013
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384018/range2384018/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384018/range2384018/1
type: Range
persistent identity: range2384018
display id: range2384018
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 173
end: 173
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation2384014
display id: annotation2384014
version: 1
top level: BBa_K1399013
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384014/range2384014/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384014/range2384014/1
type: Range
persistent identity: range2384014
display id: range2384014
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 89
end: 126
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2384031
display id: annotation2384031
version: 1
top level: BBa_K1399013
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384031/range2384031/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384031/range2384031/1
type: Range
persistent identity: range2384031
display id: range2384031
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 1089
end: 1102
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2384015
display id: annotation2384015
version: 1
top level: BBa_K1399013
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384015/range2384015/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384015/range2384015/1
type: Range
persistent identity: range2384015
display id: range2384015
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 137
end: 142
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2384033
display id: annotation2384033
version: 1
top level: BBa_K1399013
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384033/range2384033/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384033/range2384033/1
type: Range
persistent identity: range2384033
display id: range2384033
version: 1
direction: direction/reverse
top level: BBa_K1399013
start: 974
end: 1102
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2384016
display id: annotation2384016
version: 1
top level: BBa_K1399013
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384016/range2384016/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384016/range2384016/1
type: Range
persistent identity: range2384016
display id: range2384016
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation2384017
display id: annotation2384017
version: 1
top level: BBa_K1399013
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384017/range2384017/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384017/range2384017/1
type: Range
persistent identity: range2384017
display id: range2384017
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 166
end: 200
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2384019
display id: annotation2384019
version: 1
top level: BBa_K1399013
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384019/range2384019/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384019/range2384019/1
type: Range
persistent identity: range2384019
display id: range2384019
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 213
end: 216
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2384020
display id: annotation2384020
version: 1
top level: BBa_K1399013
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384020/range2384020/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384020/range2384020/1
type: Range
persistent identity: range2384020
display id: range2384020
version: 1
direction: direction/reverse
top level: BBa_K1399013
start: 209
end: 220
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2384021
display id: annotation2384021
version: 1
top level: BBa_K1399013
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384021/range2384021/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384021/range2384021/1
type: Range
persistent identity: range2384021
display id: range2384021
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 227
end: 229
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2384025
display id: annotation2384025
version: 1
top level: BBa_K1399013
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384025/range2384025/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384025/range2384025/1
type: Range
persistent identity: range2384025
display id: range2384025
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 938
end: 940
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2384032
display id: annotation2384032
version: 1
top level: BBa_K1399013
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384032/range2384032/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384032/range2384032/1
type: Range
persistent identity: range2384032
display id: range2384032
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 1095
end: 1095
orientation: inline

BBa_K1399003
type: SequenceAnnotation
persistent identity: annotation2384026
display id: annotation2384026
version: 1
top level: BBa_K1399013
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384026/range2384026/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384026/range2384026/1
type: Range
persistent identity: range2384026
display id: range2384026
version: 1
direction: direction/reverse
top level: BBa_K1399013
start: 227
end: 940
orientation: inline

DAS-ssrA tag
type: SequenceAnnotation
persistent identity: annotation2384023
display id: annotation2384023
version: 1
top level: BBa_K1399013
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384023/range2384023/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384023/range2384023/1
type: Range
persistent identity: range2384023
display id: range2384023
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 902
end: 934
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2384028
display id: annotation2384028
version: 1
top level: BBa_K1399013
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384028/range2384028/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384028/range2384028/1
type: Range
persistent identity: range2384028
display id: range2384028
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 985
end: 1028
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2384027
display id: annotation2384027
version: 1
top level: BBa_K1399013
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384027/range2384027/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384027/range2384027/1
type: Range
persistent identity: range2384027
display id: range2384027
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 974
end: 1053
orientation: inline

BBa_K283000
type: ComponentDefinition
persistent identity: BBa K283000
display id: BBa_K283000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTet-rbs-lpp_ompA_eGFP_streptavidin-double Terminators
creator: YU Bin, Qiang Qin Qin
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _383_
m user id: 0
owner id: 5494
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This part contains a red fluorescence protein followed by double terminator, the RFP has no preceding rbs sequence
mutable notes: BBa_K283000
mutable provenance: Both the RFP and the terminator are from the registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_B0034
component: BBa_B0015
component: BBa_K283005
component: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_R0040
sequence annotation: BBa_K283005
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K283000_sequence/1

BBa_B0034
type: Component
persistent identity: component2225182
display id: component2225182
version: 1
top level: BBa_K283000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0015
type: Component
persistent identity: component2225194
display id: component2225194
version: 1
top level: BBa_K283000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K283005
type: Component
persistent identity: component2225187
display id: component2225187
version: 1
top level: BBa_K283000
access: public
definition: https://synbiohub.org/public/igem/BBa_K283005/1

BBa_R0040
type: Component
persistent identity: component2225176
display id: component2225176
version: 1
top level: BBa_K283000
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2225182
display id: annotation2225182
version: 1
top level: BBa_K283000
location: https://synbiohub.org/public/igem/BBa_K283000/annotation2225182/range2225182/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K283000/annotation2225182/range2225182/1
type: Range
persistent identity: range2225182
display id: range2225182
version: 1
direction: direction/reverse
top level: BBa_K283000
start: 63
end: 74
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2225176
display id: annotation2225176
version: 1
top level: BBa_K283000
location: https://synbiohub.org/public/igem/BBa_K283000/annotation2225176/range2225176/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K283000/annotation2225176/range2225176/1
type: Range
persistent identity: range2225176
display id: range2225176
version: 1
direction: direction/forward
top level: BBa_K283000
start: 1
end: 54
orientation: inline

BBa_K283005
type: SequenceAnnotation
persistent identity: annotation2225187
display id: annotation2225187
version: 1
top level: BBa_K283000
location: https://synbiohub.org/public/igem/BBa_K283000/annotation2225187/range2225187/1
component: BBa_K283005

https://synbiohub.org/public/igem/BBa_K283000/annotation2225187/range2225187/1
type: Range
persistent identity: range2225187
display id: range2225187
version: 1
direction: direction/reverse
top level: BBa_K283000
start: 81
end: 1613
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2225194
display id: annotation2225194
version: 1
top level: BBa_K283000
location: https://synbiohub.org/public/igem/BBa_K283000/annotation2225194/range2225194/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K283000/annotation2225194/range2225194/1
type: Range
persistent identity: range2225194
display id: range2225194
version: 1
direction: direction/reverse
top level: BBa_K283000
start: 1622
end: 1750
orientation: inline

BBa_K1399019
type: ComponentDefinition
persistent identity: BBa K1399019
display id: BBa_K1399019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ptet-GFP(AAV)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Tetracycline/aTc inducible promoter with GFP reporter tagged with AAV-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.
mutable notes: Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
mutable provenance: other biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0015
component: BBa_K1399005
component: BBa_B0034
component: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_R0040
sequence annotation: BBa_K1399005
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1399019_sequence/1

BBa_B0015
type: Component
persistent identity: component2384238
display id: component2384238
version: 1
top level: BBa_K1399019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K1399005
type: Component
persistent identity: component2384231
display id: component2384231
version: 1
top level: BBa_K1399019
access: public
definition: https://synbiohub.org/public/igem/BBa_K1399005/1

BBa_B0034
type: Component
persistent identity: component2384225
display id: component2384225
version: 1
top level: BBa_K1399019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0040
type: Component
persistent identity: component2384219
display id: component2384219
version: 1
top level: BBa_K1399019
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2384225
display id: annotation2384225
version: 1
top level: BBa_K1399019
location: https://synbiohub.org/public/igem/BBa_K1399019/annotation2384225/range2384225/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1399019/annotation2384225/range2384225/1
type: Range
persistent identity: range2384225
display id: range2384225
version: 1
direction: direction/reverse
top level: BBa_K1399019
start: 63
end: 74
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2384219
display id: annotation2384219
version: 1
top level: BBa_K1399019
location: https://synbiohub.org/public/igem/BBa_K1399019/annotation2384219/range2384219/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K1399019/annotation2384219/range2384219/1
type: Range
persistent identity: range2384219
display id: range2384219
version: 1
direction: direction/forward
top level: BBa_K1399019
start: 1
end: 54
orientation: inline

BBa_K1399005
type: SequenceAnnotation
persistent identity: annotation2384231
display id: annotation2384231
version: 1
top level: BBa_K1399019
location: https://synbiohub.org/public/igem/BBa_K1399019/annotation2384231/range2384231/1
component: BBa_K1399005

https://synbiohub.org/public/igem/BBa_K1399019/annotation2384231/range2384231/1
type: Range
persistent identity: range2384231
display id: range2384231
version: 1
direction: direction/reverse
top level: BBa_K1399019
start: 81
end: 833
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2384238
display id: annotation2384238
version: 1
top level: BBa_K1399019
location: https://synbiohub.org/public/igem/BBa_K1399019/annotation2384238/range2384238/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1399019/annotation2384238/range2384238/1
type: Range
persistent identity: range2384238
display id: range2384238
version: 1
direction: direction/reverse
top level: BBa_K1399019
start: 842
end: 970
orientation: inline

BBa_I711018
type: ComponentDefinition
persistent identity: BBa I711018
display id: BBa_I711018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Acetyl-CoA -> acetoacetyl-CoA (thiolase)
creator: Ranjan Khan
created: 2007-07-15T11:00:00Z
modified: 2015-08-31T04:07:45Z
discontinued: false
dominant: false
group u list: _158_
m user id: 0
owner id: 1498
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part constitutively produces thiolase, and enzyme responsible for creating acetoacetyl-CoA from acetyl-CoA.
mutable notes: None
mutable provenance: Clostridium acetobutylicum ATCC 824
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J23119
component: BBa_B0014
component: BBa_B0030
component: BBa_I711014
sequence annotation: BBa_I711014
sequence annotation: BBa_B0014
sequence annotation: BBa_B0030
sequence annotation: BBa_J23119
sequence: https://synbiohub.org/public/igem/BBa_I711018_sequence/1

BBa_J23119
type: Component
persistent identity: component2222009
display id: component2222009
version: 1
top level: BBa_I711018
access: public
definition: https://synbiohub.org/public/igem/BBa_J23119/1

BBa_B0014
type: Component
persistent identity: component2222020
display id: component2222020
version: 1
top level: BBa_I711018
access: public
definition: https://synbiohub.org/public/igem/BBa_B0014/1

BBa_B0030
type: Component
persistent identity: component2222011
display id: component2222011
version: 1
top level: BBa_I711018
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_I711014
type: Component
persistent identity: component2222013
display id: component2222013
version: 1
top level: BBa_I711018
access: public
definition: https://synbiohub.org/public/igem/BBa_I711014/1

BBa_I711014
type: SequenceAnnotation
persistent identity: annotation2222013
display id: annotation2222013
version: 1
top level: BBa_I711018
location: https://synbiohub.org/public/igem/BBa_I711018/annotation2222013/range2222013/1
component: BBa_I711014

https://synbiohub.org/public/igem/BBa_I711018/annotation2222013/range2222013/1
type: Range
persistent identity: range2222013
display id: range2222013
version: 1
direction: direction/reverse
top level: BBa_I711018
start: 65
end: 1240
orientation: inline

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2222020
display id: annotation2222020
version: 1
top level: BBa_I711018
location: https://synbiohub.org/public/igem/BBa_I711018/annotation2222020/range2222020/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_I711018/annotation2222020/range2222020/1
type: Range
persistent identity: range2222020
display id: range2222020
version: 1
direction: direction/reverse
top level: BBa_I711018
start: 1249
end: 1343
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2222011
display id: annotation2222011
version: 1
top level: BBa_I711018
location: https://synbiohub.org/public/igem/BBa_I711018/annotation2222011/range2222011/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_I711018/annotation2222011/range2222011/1
type: Range
persistent identity: range2222011
display id: range2222011
version: 1
direction: direction/forward
top level: BBa_I711018
start: 44
end: 58
orientation: inline

BBa_J23119
type: SequenceAnnotation
persistent identity: annotation2222009
display id: annotation2222009
version: 1
top level: BBa_I711018
location: https://synbiohub.org/public/igem/BBa_I711018/annotation2222009/range2222009/1
component: BBa_J23119

https://synbiohub.org/public/igem/BBa_I711018/annotation2222009/range2222009/1
type: Range
persistent identity: range2222009
display id: range2222009
version: 1
direction: direction/reverse
top level: BBa_I711018
start: 1
end: 35
orientation: inline

BBa_K283003
type: ComponentDefinition
persistent identity: BBa K283003
display id: BBa_K283003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTet-rbs-chez_histag-double terminator
creator: Wei Ling , Nelson Chu, Shi Lei
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _383_
m user id: 0
owner id: 5494
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part consists of a fusion protein invisine-RFP with a double terminator. There is no preceding rbs on the protein coding region.
mutable notes: BBa_K283003
mutable provenance: The invisine coding region is self-designed and the terminators are from the registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0012
component: BBa_B0010
component: BBa_B0034
component: BBa_K283008
component: BBa_R0040
sequence annotation: BBa_K283008
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_R0040
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_K283003_sequence/1

BBa_B0012
type: Component
persistent identity: component2047855
display id: component2047855
version: 1
top level: BBa_K283003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component2047853
display id: component2047853
version: 1
top level: BBa_K283003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component2047848
display id: component2047848
version: 1
top level: BBa_K283003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K283008
type: Component
persistent identity: component2047852
display id: component2047852
version: 1
top level: BBa_K283003
access: public
definition: https://synbiohub.org/public/igem/BBa_K283008/1

BBa_R0040
type: Component
persistent identity: component2047842
display id: component2047842
version: 1
top level: BBa_K283003
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_K283008
type: SequenceAnnotation
persistent identity: annotation2047852
display id: annotation2047852
version: 1
top level: BBa_K283003
location: https://synbiohub.org/public/igem/BBa_K283003/annotation2047852/range2047852/1
component: BBa_K283008

https://synbiohub.org/public/igem/BBa_K283003/annotation2047852/range2047852/1
type: Range
persistent identity: range2047852
display id: range2047852
version: 1
direction: direction/reverse
top level: BBa_K283003
start: 81
end: 793
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2047855
display id: annotation2047855
version: 1
top level: BBa_K283003
location: https://synbiohub.org/public/igem/BBa_K283003/annotation2047855/range2047855/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K283003/annotation2047855/range2047855/1
type: Range
persistent identity: range2047855
display id: range2047855
version: 1
direction: direction/forward
top level: BBa_K283003
start: 890
end: 930
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2047848
display id: annotation2047848
version: 1
top level: BBa_K283003
location: https://synbiohub.org/public/igem/BBa_K283003/annotation2047848/range2047848/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K283003/annotation2047848/range2047848/1
type: Range
persistent identity: range2047848
display id: range2047848
version: 1
direction: direction/reverse
top level: BBa_K283003
start: 63
end: 74
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2047842
display id: annotation2047842
version: 1
top level: BBa_K283003
location: https://synbiohub.org/public/igem/BBa_K283003/annotation2047842/range2047842/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K283003/annotation2047842/range2047842/1
type: Range
persistent identity: range2047842
display id: range2047842
version: 1
direction: direction/forward
top level: BBa_K283003
start: 1
end: 54
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2047853
display id: annotation2047853
version: 1
top level: BBa_K283003
location: https://synbiohub.org/public/igem/BBa_K283003/annotation2047853/range2047853/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K283003/annotation2047853/range2047853/1
type: Range
persistent identity: range2047853
display id: range2047853
version: 1
direction: direction/forward
top level: BBa_K283003
start: 802
end: 881
orientation: inline

BBa_K1399015
type: ComponentDefinition
persistent identity: BBa K1399015
display id: BBa_K1399015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac-GFP(LVA)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Lactose/IPTG inducible promoter with GFP reporter tagged with AAV-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.
mutable notes: Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
mutable provenance: other biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1399004
component: BBa_R0010
component: BBa_B0015
component: BBa_B0034
sequence annotation: BBa_B0015
sequence annotation: BBa_K1399004
sequence annotation: BBa_B0034
sequence annotation: BBa_R0010
sequence: https://synbiohub.org/public/igem/BBa_K1399015_sequence/1

BBa_K1399004
type: Component
persistent identity: component2384078
display id: component2384078
version: 1
top level: BBa_K1399015
access: public
definition: https://synbiohub.org/public/igem/BBa_K1399004/1

BBa_R0010
type: Component
persistent identity: component2384064
display id: component2384064
version: 1
top level: BBa_K1399015
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0015
type: Component
persistent identity: component2384085
display id: component2384085
version: 1
top level: BBa_K1399015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0034
type: Component
persistent identity: component2384072
display id: component2384072
version: 1
top level: BBa_K1399015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2384085
display id: annotation2384085
version: 1
top level: BBa_K1399015
location: https://synbiohub.org/public/igem/BBa_K1399015/annotation2384085/range2384085/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1399015/annotation2384085/range2384085/1
type: Range
persistent identity: range2384085
display id: range2384085
version: 1
direction: direction/reverse
top level: BBa_K1399015
start: 988
end: 1116
orientation: inline

BBa_K1399004
type: SequenceAnnotation
persistent identity: annotation2384078
display id: annotation2384078
version: 1
top level: BBa_K1399015
location: https://synbiohub.org/public/igem/BBa_K1399015/annotation2384078/range2384078/1
component: BBa_K1399004

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type: Range
persistent identity: range2384078
display id: range2384078
version: 1
direction: direction/reverse
top level: BBa_K1399015
start: 227
end: 979
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2384072
display id: annotation2384072
version: 1
top level: BBa_K1399015
location: https://synbiohub.org/public/igem/BBa_K1399015/annotation2384072/range2384072/1
component: BBa_B0034

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type: Range
persistent identity: range2384072
display id: range2384072
version: 1
direction: direction/reverse
top level: BBa_K1399015
start: 209
end: 220
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2384064
display id: annotation2384064
version: 1
top level: BBa_K1399015
location: https://synbiohub.org/public/igem/BBa_K1399015/annotation2384064/range2384064/1
component: BBa_R0010

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type: Range
persistent identity: range2384064
display id: range2384064
version: 1
direction: direction/forward
top level: BBa_K1399015
start: 1
end: 200
orientation: inline

BBa_K1399016
type: ComponentDefinition
persistent identity: BBa K1399016
display id: BBa_K1399016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac-GFP(LAA)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Lactose/IPTG inducible promoter with GFP reporter tagged with AAV-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.
mutable notes: Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
mutable provenance: other biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0015
component: BBa_R0010
component: BBa_B0034
component: BBa_K1399006
sequence annotation: BBa_R0010
sequence annotation: BBa_B0015
sequence annotation: BBa_K1399006
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1399016_sequence/1

BBa_B0015
type: Component
persistent identity: component2384166
display id: component2384166
version: 1
top level: BBa_K1399016
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_R0010
type: Component
persistent identity: component2384145
display id: component2384145
version: 1
top level: BBa_K1399016
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0034
type: Component
persistent identity: component2384153
display id: component2384153
version: 1
top level: BBa_K1399016
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1399006
type: Component
persistent identity: component2384159
display id: component2384159
version: 1
top level: BBa_K1399016
access: public
definition: https://synbiohub.org/public/igem/BBa_K1399006/1

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2384145
display id: annotation2384145
version: 1
top level: BBa_K1399016
location: https://synbiohub.org/public/igem/BBa_K1399016/annotation2384145/range2384145/1
component: BBa_R0010

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type: Range
persistent identity: range2384145
display id: range2384145
version: 1
direction: direction/forward
top level: BBa_K1399016
start: 1
end: 200
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2384166
display id: annotation2384166
version: 1
top level: BBa_K1399016
location: https://synbiohub.org/public/igem/BBa_K1399016/annotation2384166/range2384166/1
component: BBa_B0015

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type: Range
persistent identity: range2384166
display id: range2384166
version: 1
direction: direction/reverse
top level: BBa_K1399016
start: 988
end: 1116
orientation: inline

BBa_K1399006
type: SequenceAnnotation
persistent identity: annotation2384159
display id: annotation2384159
version: 1
top level: BBa_K1399016
location: https://synbiohub.org/public/igem/BBa_K1399016/annotation2384159/range2384159/1
component: BBa_K1399006

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type: Range
persistent identity: range2384159
display id: range2384159
version: 1
direction: direction/reverse
top level: BBa_K1399016
start: 227
end: 979
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2384153
display id: annotation2384153
version: 1
top level: BBa_K1399016
location: https://synbiohub.org/public/igem/BBa_K1399016/annotation2384153/range2384153/1
component: BBa_B0034

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type: Range
persistent identity: range2384153
display id: range2384153
version: 1
direction: direction/reverse
top level: BBa_K1399016
start: 209
end: 220
orientation: inline

BBa_K1399017
type: ComponentDefinition
persistent identity: BBa K1399017
display id: BBa_K1399017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac-GFP(DAS+2)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Lactose/IPTG inducible promoter with GFP reporter tagged with NYADAS-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.
mutable notes: Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
mutable provenance: other biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0015
component: BBa_B0034
component: BBa_K1399007
component: BBa_R0010
sequence annotation: BBa_B0015
sequence annotation: BBa_R0010
sequence annotation: BBa_K1399007
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1399017_sequence/1

BBa_B0015
type: Component
persistent identity: component2384196
display id: component2384196
version: 1
top level: BBa_K1399017
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0034
type: Component
persistent identity: component2384183
display id: component2384183
version: 1
top level: BBa_K1399017
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1399007
type: Component
persistent identity: component2384189
display id: component2384189
version: 1
top level: BBa_K1399017
access: public
definition: https://synbiohub.org/public/igem/BBa_K1399007/1

BBa_R0010
type: Component
persistent identity: component2384175
display id: component2384175
version: 1
top level: BBa_K1399017
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2384196
display id: annotation2384196
version: 1
top level: BBa_K1399017
location: https://synbiohub.org/public/igem/BBa_K1399017/annotation2384196/range2384196/1
component: BBa_B0015

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type: Range
persistent identity: range2384196
display id: range2384196
version: 1
direction: direction/reverse
top level: BBa_K1399017
start: 994
end: 1122
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2384175
display id: annotation2384175
version: 1
top level: BBa_K1399017
location: https://synbiohub.org/public/igem/BBa_K1399017/annotation2384175/range2384175/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K1399017/annotation2384175/range2384175/1
type: Range
persistent identity: range2384175
display id: range2384175
version: 1
direction: direction/forward
top level: BBa_K1399017
start: 1
end: 200
orientation: inline

BBa_K1399007
type: SequenceAnnotation
persistent identity: annotation2384189
display id: annotation2384189
version: 1
top level: BBa_K1399017
location: https://synbiohub.org/public/igem/BBa_K1399017/annotation2384189/range2384189/1
component: BBa_K1399007

https://synbiohub.org/public/igem/BBa_K1399017/annotation2384189/range2384189/1
type: Range
persistent identity: range2384189
display id: range2384189
version: 1
direction: direction/reverse
top level: BBa_K1399017
start: 227
end: 985
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2384183
display id: annotation2384183
version: 1
top level: BBa_K1399017
location: https://synbiohub.org/public/igem/BBa_K1399017/annotation2384183/range2384183/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1399017/annotation2384183/range2384183/1
type: Range
persistent identity: range2384183
display id: range2384183
version: 1
direction: direction/reverse
top level: BBa_K1399017
start: 209
end: 220
orientation: inline

Pulsegen o
type: ComponentDefinition
persistent identity: BBa J03101
display id: BBa_J03101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cI repressor under LacI promoter
creator: James Godman
created: 2005-10-04T11:00:00Z
modified: 2015-08-31T04:08:13Z
discontinued: false
dominant: false
group u list: _15_
m user id: 0
owner id: 405
owning group id: 15
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_C0051
component: BBa_B0012
component: BBa_B0034
component: BBa_R0010
component: BBa_B0011
sequence annotation: BBa_B0012
sequence annotation: BBa_R0010
sequence annotation: BBa_B0034
sequence annotation: BBa_C0051
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_J03101_sequence/1

BBa_C0051
type: Component
persistent identity: component1718168
display id: component1718168
version: 1
top level: Pulsegen o
access: public
definition: https://synbiohub.org/public/igem/BBa_C0051/1

BBa_B0012
type: Component
persistent identity: component1718178
display id: component1718178
version: 1
top level: Pulsegen o
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component1718158
display id: component1718158
version: 1
top level: Pulsegen o
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0010
type: Component
persistent identity: component1718147
display id: component1718147
version: 1
top level: Pulsegen o
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0011
type: Component
persistent identity: component1718186
display id: component1718186
version: 1
top level: Pulsegen o
access: public
definition: https://synbiohub.org/public/igem/BBa_B0011/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1718178
display id: annotation1718178
version: 1
top level: Pulsegen o
location: https://synbiohub.org/public/igem/BBa_J03101/annotation1718178/range1718178/1
component: BBa_B0012

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type: Range
persistent identity: range1718178
display id: range1718178
version: 1
direction: direction/forward
top level: Pulsegen o
start: 1010
end: 1050
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1718147
display id: annotation1718147
version: 1
top level: Pulsegen o
location: https://synbiohub.org/public/igem/BBa_J03101/annotation1718147/range1718147/1
component: BBa_R0010

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type: Range
persistent identity: range1718147
display id: range1718147
version: 1
direction: direction/forward
top level: Pulsegen o
start: 1
end: 200
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1718158
display id: annotation1718158
version: 1
top level: Pulsegen o
location: https://synbiohub.org/public/igem/BBa_J03101/annotation1718158/range1718158/1
component: BBa_B0034

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type: Range
persistent identity: range1718158
display id: range1718158
version: 1
direction: direction/forward
top level: Pulsegen o
start: 209
end: 220
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation1718168
display id: annotation1718168
version: 1
top level: Pulsegen o
location: https://synbiohub.org/public/igem/BBa_J03101/annotation1718168/range1718168/1
component: BBa_C0051

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type: Range
persistent identity: range1718168
display id: range1718168
version: 1
direction: direction/forward
top level: Pulsegen o
start: 227
end: 976
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation1718186
display id: annotation1718186
version: 1
top level: Pulsegen o
location: https://synbiohub.org/public/igem/BBa_J03101/annotation1718186/range1718186/1
component: BBa_B0011

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type: Range
persistent identity: range1718186
display id: range1718186
version: 1
direction: direction/forward
top level: Pulsegen o
start: 1059
end: 1104
orientation: inline

B-carotene
type: ComponentDefinition
persistent identity: BBa K343006
display id: BBa_K343006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Expresses B-carotene monooxygenase on a constitutive promotor
creator: Christian Kurtzhals
created: 2010-10-13T11:00:00Z
modified: 2015-05-08T01:12:08Z
discontinued: false
dominant: false
experience: experience/None
group u list: _465_
m user id: 0
owner id: 6083
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This generator expresses a beta-carotene 15,15'-monooxygenase,on a constitutive promotor, that catalyses retinal production from beta-carotene via the following reaction: beta-carotene + O2 = 2 retinal
mutable notes: The brick requires B-carotene supply to function. This can either be supplied externally, or alternatively the BBa_K274210 B-carotene generator will induce full B-carotene synthesis in E. coli.
mutable provenance: The ninaB gene was originally cloned from drosophila melanogaster, and cDNA was aquired for production of the biobrick from the Drosophila Genome Ressource Center. Promotor+rbs brick BBa_J13002 and terminator BBa_B0015 were used.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0012
component: BBa_R0040
component: BBa_B0010
component: BBa_K343001
component: BBa_B0034
sequence annotation: BBa_K343001
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_K343006_sequence/1

BBa_B0012
type: Component
persistent identity: component2085991
display id: component2085991
version: 1
top level: B-carotene
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0040
type: Component
persistent identity: component2085978
display id: component2085978
version: 1
top level: B-carotene
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0010
type: Component
persistent identity: component2085989
display id: component2085989
version: 1
top level: B-carotene
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_K343001
type: Component
persistent identity: component2085988
display id: component2085988
version: 1
top level: B-carotene
access: public
definition: https://synbiohub.org/public/igem/BBa_K343001/1

BBa_B0034
type: Component
persistent identity: component2085984
display id: component2085984
version: 1
top level: B-carotene
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K343001
type: SequenceAnnotation
persistent identity: annotation2085988
display id: annotation2085988
version: 1
top level: B-carotene
location: https://synbiohub.org/public/igem/BBa_K343006/annotation2085988/range2085988/1
component: BBa_K343001

https://synbiohub.org/public/igem/BBa_K343006/annotation2085988/range2085988/1
type: Range
persistent identity: range2085988
display id: range2085988
version: 1
direction: direction/reverse
top level: B-carotene
start: 81
end: 1943
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2085978
display id: annotation2085978
version: 1
top level: B-carotene
location: https://synbiohub.org/public/igem/BBa_K343006/annotation2085978/range2085978/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K343006/annotation2085978/range2085978/1
type: Range
persistent identity: range2085978
display id: range2085978
version: 1
direction: direction/forward
top level: B-carotene
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2085984
display id: annotation2085984
version: 1
top level: B-carotene
location: https://synbiohub.org/public/igem/BBa_K343006/annotation2085984/range2085984/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K343006/annotation2085984/range2085984/1
type: Range
persistent identity: range2085984
display id: range2085984
version: 1
direction: direction/reverse
top level: B-carotene
start: 63
end: 74
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2085991
display id: annotation2085991
version: 1
top level: B-carotene
location: https://synbiohub.org/public/igem/BBa_K343006/annotation2085991/range2085991/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K343006/annotation2085991/range2085991/1
type: Range
persistent identity: range2085991
display id: range2085991
version: 1
direction: direction/forward
top level: B-carotene
start: 2040
end: 2080
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2085989
display id: annotation2085989
version: 1
top level: B-carotene
location: https://synbiohub.org/public/igem/BBa_K343006/annotation2085989/range2085989/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K343006/annotation2085989/range2085989/1
type: Range
persistent identity: range2085989
display id: range2085989
version: 1
direction: direction/forward
top level: B-carotene
start: 1952
end: 2031
orientation: inline

BBa_I711005
type: ComponentDefinition
persistent identity: BBa I711005
display id: BBa_I711005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Melanogenesis with red light trigger
creator: VGEM 2007
created: 2007-06-04T11:00:00Z
modified: 2015-08-31T04:07:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _158_
m user id: 0
owner id: 1498
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is triggered by BBa_1711006 (which detects red light) and produces tyrosinase and ORF438, two proteins known to be important for melanogenesis.
mutable notes: The promoter region may have to be modified to be activated by BBa_I711006.
mutable provenance: S. antibiotica
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_I711004
component: BBa_B0011
component: BBa_B0030
component: BBa_R0084
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_I711004
sequence annotation: BBa_B0011
sequence annotation: BBa_R0084
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_I711005_sequence/1

BBa_I711004
type: Component
persistent identity: component1934546
display id: component1934546
version: 1
top level: BBa_I711005
access: public
definition: https://synbiohub.org/public/igem/BBa_I711004/1

BBa_B0011
type: Component
persistent identity: component1934551
display id: component1934551
version: 1
top level: BBa_I711005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0011/1

BBa_B0030
type: Component
persistent identity: component1934544
display id: component1934544
version: 1
top level: BBa_I711005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_R0084
type: Component
persistent identity: component1934542
display id: component1934542
version: 1
top level: BBa_I711005
access: public
definition: https://synbiohub.org/public/igem/BBa_R0084/1

BBa_B0012
type: Component
persistent identity: component1934547
display id: component1934547
version: 1
top level: BBa_I711005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1934547
display id: annotation1934547
version: 1
top level: BBa_I711005
location: https://synbiohub.org/public/igem/BBa_I711005/annotation1934547/range1934547/1
component: BBa_B0012

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type: Range
persistent identity: range1934547
display id: range1934547
version: 1
direction: direction/forward
top level: BBa_I711005
start: 1441
end: 1481
orientation: inline

BBa_I711004
type: SequenceAnnotation
persistent identity: annotation1934546
display id: annotation1934546
version: 1
top level: BBa_I711005
location: https://synbiohub.org/public/igem/BBa_I711005/annotation1934546/range1934546/1
component: BBa_I711004

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type: Range
persistent identity: range1934546
display id: range1934546
version: 1
direction: direction/reverse
top level: BBa_I711005
start: 138
end: 1432
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation1934551
display id: annotation1934551
version: 1
top level: BBa_I711005
location: https://synbiohub.org/public/igem/BBa_I711005/annotation1934551/range1934551/1
component: BBa_B0011

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type: Range
persistent identity: range1934551
display id: range1934551
version: 1
direction: direction/forward
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start: 1490
end: 1535
orientation: inline

BBa_R0084
type: SequenceAnnotation
persistent identity: annotation1934542
display id: annotation1934542
version: 1
top level: BBa_I711005
location: https://synbiohub.org/public/igem/BBa_I711005/annotation1934542/range1934542/1
component: BBa_R0084

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type: Range
persistent identity: range1934542
display id: range1934542
version: 1
direction: direction/reverse
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BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1934544
display id: annotation1934544
version: 1
top level: BBa_I711005
location: https://synbiohub.org/public/igem/BBa_I711005/annotation1934544/range1934544/1
component: BBa_B0030

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type: Range
persistent identity: range1934544
display id: range1934544
version: 1
direction: direction/forward
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start: 117
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orientation: inline

BBa_I711001
type: ComponentDefinition
persistent identity: BBa I711001
display id: BBa_I711001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: generates the tyrosinase for melanin biosynthesis
creator: Ron Bauerle
created: 2007-05-25T11:00:00Z
modified: 2015-08-31T04:07:45Z
discontinued: false
dominant: false
group u list: _158_
m user id: 0
owner id: 1496
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Constitutive promoter
mutable notes: no design considerations
mutable provenance: Registry promoter, RBS, and terminator. this is the generator for tyrosinase gene
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J23119
component: BBa_B0012
component: BBa_I711000
component: BBa_B0011
component: BBa_B0030
sequence annotation: BBa_I711000
sequence annotation: BBa_B0011
sequence annotation: BBa_B0030
sequence annotation: BBa_B0012
sequence annotation: BBa_J23119
sequence: https://synbiohub.org/public/igem/BBa_I711001_sequence/1

BBa_J23119
type: Component
persistent identity: component1933852
display id: component1933852
version: 1
top level: BBa_I711001
access: public
definition: https://synbiohub.org/public/igem/BBa_J23119/1

BBa_B0012
type: Component
persistent identity: component1933858
display id: component1933858
version: 1
top level: BBa_I711001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_I711000
type: Component
persistent identity: component1933857
display id: component1933857
version: 1
top level: BBa_I711001
access: public
definition: https://synbiohub.org/public/igem/BBa_I711000/1

BBa_B0011
type: Component
persistent identity: component1933862
display id: component1933862
version: 1
top level: BBa_I711001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0011/1

BBa_B0030
type: Component
persistent identity: component1933854
display id: component1933854
version: 1
top level: BBa_I711001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_I711000
type: SequenceAnnotation
persistent identity: annotation1933857
display id: annotation1933857
version: 1
top level: BBa_I711001
location: https://synbiohub.org/public/igem/BBa_I711001/annotation1933857/range1933857/1
component: BBa_I711000

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type: Range
persistent identity: range1933857
display id: range1933857
version: 1
direction: direction/reverse
top level: BBa_I711001
start: 65
end: 889
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation1933862
display id: annotation1933862
version: 1
top level: BBa_I711001
location: https://synbiohub.org/public/igem/BBa_I711001/annotation1933862/range1933862/1
component: BBa_B0011

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type: Range
persistent identity: range1933862
display id: range1933862
version: 1
direction: direction/forward
top level: BBa_I711001
start: 947
end: 992
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1933854
display id: annotation1933854
version: 1
top level: BBa_I711001
location: https://synbiohub.org/public/igem/BBa_I711001/annotation1933854/range1933854/1
component: BBa_B0030

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type: Range
persistent identity: range1933854
display id: range1933854
version: 1
direction: direction/forward
top level: BBa_I711001
start: 44
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orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1933858
display id: annotation1933858
version: 1
top level: BBa_I711001
location: https://synbiohub.org/public/igem/BBa_I711001/annotation1933858/range1933858/1
component: BBa_B0012

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type: Range
persistent identity: range1933858
display id: range1933858
version: 1
direction: direction/forward
top level: BBa_I711001
start: 898
end: 938
orientation: inline

BBa_J23119
type: SequenceAnnotation
persistent identity: annotation1933852
display id: annotation1933852
version: 1
top level: BBa_I711001
location: https://synbiohub.org/public/igem/BBa_I711001/annotation1933852/range1933852/1
component: BBa_J23119

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type: Range
persistent identity: range1933852
display id: range1933852
version: 1
direction: direction/reverse
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start: 1
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orientation: inline

BBa_K1399021
type: ComponentDefinition
persistent identity: BBa K1399021
display id: BBa_K1399021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ptet-GFP(LAA)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Tetracycline/aTc inducible promoter with GFP reporter tagged with LAA-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.
mutable notes: Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
mutable provenance: other parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0034
component: BBa_K1399006
component: BBa_B0015
component: BBa_R0040
sequence annotation: BBa_K1399006
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1399021_sequence/1

BBa_B0034
type: Component
persistent identity: component2384265
display id: component2384265
version: 1
top level: BBa_K1399021
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1399006
type: Component
persistent identity: component2384271
display id: component2384271
version: 1
top level: BBa_K1399021
access: public
definition: https://synbiohub.org/public/igem/BBa_K1399006/1

BBa_B0015
type: Component
persistent identity: component2384278
display id: component2384278
version: 1
top level: BBa_K1399021
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_R0040
type: Component
persistent identity: component2384259
display id: component2384259
version: 1
top level: BBa_K1399021
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_K1399006
type: SequenceAnnotation
persistent identity: annotation2384271
display id: annotation2384271
version: 1
top level: BBa_K1399021
location: https://synbiohub.org/public/igem/BBa_K1399021/annotation2384271/range2384271/1
component: BBa_K1399006

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type: Range
persistent identity: range2384271
display id: range2384271
version: 1
direction: direction/reverse
top level: BBa_K1399021
start: 81
end: 833
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2384259
display id: annotation2384259
version: 1
top level: BBa_K1399021
location: https://synbiohub.org/public/igem/BBa_K1399021/annotation2384259/range2384259/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K1399021/annotation2384259/range2384259/1
type: Range
persistent identity: range2384259
display id: range2384259
version: 1
direction: direction/forward
top level: BBa_K1399021
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2384265
display id: annotation2384265
version: 1
top level: BBa_K1399021
location: https://synbiohub.org/public/igem/BBa_K1399021/annotation2384265/range2384265/1
component: BBa_B0034

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type: Range
persistent identity: range2384265
display id: range2384265
version: 1
direction: direction/reverse
top level: BBa_K1399021
start: 63
end: 74
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2384278
display id: annotation2384278
version: 1
top level: BBa_K1399021
location: https://synbiohub.org/public/igem/BBa_K1399021/annotation2384278/range2384278/1
component: BBa_B0015

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type: Range
persistent identity: range2384278
display id: range2384278
version: 1
direction: direction/reverse
top level: BBa_K1399021
start: 842
end: 970
orientation: inline

WOG
type: ComponentDefinition
persistent identity: BBa J45100
display id: BBa_J45100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Wintergreen odor enzyme (BSMT1) generator
creator: Boyuan Zhu
created: 2006-08-08T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 974
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is the wintergreen generating device hooked up to a constitutive promoter.
mutable notes: See J45099
mutable provenance: This is made from a biobrick promoter and our biobrick wintergreen generating device.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0012
component: BBa_J45004
component: BBa_B0010
component: BBa_R0040
component: BBa_B0030
sequence annotation: BBa_R0040
sequence annotation: BBa_B0010
sequence annotation: BBa_J45004
sequence annotation: BBa_B0030
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J45100_sequence/1

BBa_B0012
type: Component
persistent identity: component1894594
display id: component1894594
version: 1
top level: WOG
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_J45004
type: Component
persistent identity: component1894591
display id: component1894591
version: 1
top level: WOG
access: public
definition: https://synbiohub.org/public/igem/BBa_J45004/1

BBa_B0010
type: Component
persistent identity: component1894592
display id: component1894592
version: 1
top level: WOG
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0040
type: Component
persistent identity: component1894581
display id: component1894581
version: 1
top level: WOG
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0030
type: Component
persistent identity: component1894586
display id: component1894586
version: 1
top level: WOG
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1894581
display id: annotation1894581
version: 1
top level: WOG
location: https://synbiohub.org/public/igem/BBa_J45100/annotation1894581/range1894581/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_J45100/annotation1894581/range1894581/1
type: Range
persistent identity: range1894581
display id: range1894581
version: 1
direction: direction/forward
top level: WOG
start: 1
end: 54
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1894592
display id: annotation1894592
version: 1
top level: WOG
location: https://synbiohub.org/public/igem/BBa_J45100/annotation1894592/range1894592/1
component: BBa_B0010

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type: Range
persistent identity: range1894592
display id: range1894592
version: 1
direction: direction/forward
top level: WOG
start: 1166
end: 1245
orientation: inline

BBa_J45004
type: SequenceAnnotation
persistent identity: annotation1894591
display id: annotation1894591
version: 1
top level: WOG
location: https://synbiohub.org/public/igem/BBa_J45100/annotation1894591/range1894591/1
component: BBa_J45004

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type: Range
persistent identity: range1894591
display id: range1894591
version: 1
direction: direction/reverse
top level: WOG
start: 84
end: 1157
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1894586
display id: annotation1894586
version: 1
top level: WOG
location: https://synbiohub.org/public/igem/BBa_J45100/annotation1894586/range1894586/1
component: BBa_B0030

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type: Range
persistent identity: range1894586
display id: range1894586
version: 1
direction: direction/forward
top level: WOG
start: 63
end: 77
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1894594
display id: annotation1894594
version: 1
top level: WOG
location: https://synbiohub.org/public/igem/BBa_J45100/annotation1894594/range1894594/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J45100/annotation1894594/range1894594/1
type: Range
persistent identity: range1894594
display id: range1894594
version: 1
direction: direction/forward
top level: WOG
start: 1254
end: 1294
orientation: inline

BBa_K1399018
type: ComponentDefinition
persistent identity: BBa K1399018
display id: BBa_K1399018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac-GFP(DAS)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Lactose/IPTG inducible promoter with GFP reporter tagged with DAS-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity. Presence of SspB is crucial for effective protein degradation.
mutable notes: Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
mutable provenance: other biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1399008
component: BBa_R0010
component: BBa_B0015
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_R0010
sequence annotation: BBa_K1399008
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1399018_sequence/1

BBa_K1399008
type: Component
persistent identity: component2384211
display id: component2384211
version: 1
top level: BBa_K1399018
access: public
definition: https://synbiohub.org/public/igem/BBa_K1399008/1

BBa_R0010
type: Component
persistent identity: component2384197
display id: component2384197
version: 1
top level: BBa_K1399018
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0015
type: Component
persistent identity: component2384218
display id: component2384218
version: 1
top level: BBa_K1399018
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0034
type: Component
persistent identity: component2384205
display id: component2384205
version: 1
top level: BBa_K1399018
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2384205
display id: annotation2384205
version: 1
top level: BBa_K1399018
location: https://synbiohub.org/public/igem/BBa_K1399018/annotation2384205/range2384205/1
component: BBa_B0034

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type: Range
persistent identity: range2384205
display id: range2384205
version: 1
direction: direction/reverse
top level: BBa_K1399018
start: 209
end: 220
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2384197
display id: annotation2384197
version: 1
top level: BBa_K1399018
location: https://synbiohub.org/public/igem/BBa_K1399018/annotation2384197/range2384197/1
component: BBa_R0010

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type: Range
persistent identity: range2384197
display id: range2384197
version: 1
direction: direction/forward
top level: BBa_K1399018
start: 1
end: 200
orientation: inline

BBa_K1399008
type: SequenceAnnotation
persistent identity: annotation2384211
display id: annotation2384211
version: 1
top level: BBa_K1399018
location: https://synbiohub.org/public/igem/BBa_K1399018/annotation2384211/range2384211/1
component: BBa_K1399008

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type: Range
persistent identity: range2384211
display id: range2384211
version: 1
direction: direction/reverse
top level: BBa_K1399018
start: 227
end: 979
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2384218
display id: annotation2384218
version: 1
top level: BBa_K1399018
location: https://synbiohub.org/public/igem/BBa_K1399018/annotation2384218/range2384218/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1399018/annotation2384218/range2384218/1
type: Range
persistent identity: range2384218
display id: range2384218
version: 1
direction: direction/reverse
top level: BBa_K1399018
start: 988
end: 1116
orientation: inline

SG
type: ComponentDefinition
persistent identity: BBa J45320
display id: BBa_J45320
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Salicylate generator
creator: Andr?? Green II
created: 2006-10-19T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 977
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Protein generator converting PoPS to the prot BSMT. The BSMT enzyme converts benzoic acid/salicylic acid to methyl benzoate and methyl salicylate.
mutable notes: None
mutable provenance: This part is phBSMT1 from Petunia x hybrida (GenBank). See part design and discussion for more information.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_R0011
component: BBa_J45017
component: BBa_B0010
component: BBa_B0032
component: BBa_B0012
sequence annotation: BBa_R0011
sequence annotation: BBa_B0032
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_J45017
sequence: https://synbiohub.org/public/igem/BBa_J45320_sequence/1

BBa_R0011
type: Component
persistent identity: component1960945
display id: component1960945
version: 1
top level: SG
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_J45017
type: Component
persistent identity: component1960958
display id: component1960958
version: 1
top level: SG
access: public
definition: https://synbiohub.org/public/igem/BBa_J45017/1

BBa_B0010
type: Component
persistent identity: component1960959
display id: component1960959
version: 1
top level: SG
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0032
type: Component
persistent identity: component1960951
display id: component1960951
version: 1
top level: SG
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_B0012
type: Component
persistent identity: component1960961
display id: component1960961
version: 1
top level: SG
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1960945
display id: annotation1960945
version: 1
top level: SG
location: https://synbiohub.org/public/igem/BBa_J45320/annotation1960945/range1960945/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_J45320/annotation1960945/range1960945/1
type: Range
persistent identity: range1960945
display id: range1960945
version: 1
direction: direction/forward
top level: SG
start: 1
end: 54
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1960951
display id: annotation1960951
version: 1
top level: SG
location: https://synbiohub.org/public/igem/BBa_J45320/annotation1960951/range1960951/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_J45320/annotation1960951/range1960951/1
type: Range
persistent identity: range1960951
display id: range1960951
version: 1
direction: direction/forward
top level: SG
start: 64
end: 76
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1960959
display id: annotation1960959
version: 1
top level: SG
location: https://synbiohub.org/public/igem/BBa_J45320/annotation1960959/range1960959/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J45320/annotation1960959/range1960959/1
type: Range
persistent identity: range1960959
display id: range1960959
version: 1
direction: direction/forward
top level: SG
start: 1827
end: 1906
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1960961
display id: annotation1960961
version: 1
top level: SG
location: https://synbiohub.org/public/igem/BBa_J45320/annotation1960961/range1960961/1
component: BBa_B0012

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type: Range
persistent identity: range1960961
display id: range1960961
version: 1
direction: direction/forward
top level: SG
start: 1915
end: 1955
orientation: inline

BBa_J45017
type: SequenceAnnotation
persistent identity: annotation1960958
display id: annotation1960958
version: 1
top level: SG
location: https://synbiohub.org/public/igem/BBa_J45320/annotation1960958/range1960958/1
component: BBa_J45017

https://synbiohub.org/public/igem/BBa_J45320/annotation1960958/range1960958/1
type: Range
persistent identity: range1960958
display id: range1960958
version: 1
direction: direction/reverse
top level: SG
start: 83
end: 1818
orientation: inline

BBa_J45220
type: ComponentDefinition
persistent identity: BBa J45220
display id: BBa_J45220
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Banana odor generator
creator: Andr?? Green II
created: 2006-10-19T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 977
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Protein generator converting PoPS to the protein ATF1. ATFI encodes the enzyme alcohol acetyltransferase I (AATase I), which produces acetate esters from acetyl CoA and alcohols. This part is similar to BBa_J45200, except that it uses the B0032 RBS instead of B0030 (which is in BBa_J45200).
mutable notes: This part is the ATF sequence BBa_45J006 with a single point mutation at base pair 1148 (T -> C). Despite the mutation, the enzyme is still functional (kinetic studies have not been done to determine realitive functionality).
mutable provenance: None.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0010
component: BBa_B0012
component: BBa_R0011
component: BBa_J45014
component: BBa_B0032
sequence annotation: BBa_B0012
sequence annotation: BBa_J45014
sequence annotation: BBa_B0032
sequence annotation: BBa_B0010
sequence annotation: BBa_R0011
sequence: https://synbiohub.org/public/igem/BBa_J45220_sequence/1

BBa_B0010
type: Component
persistent identity: component1903719
display id: component1903719
version: 1
top level: BBa_J45220
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1903721
display id: component1903721
version: 1
top level: BBa_J45220
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0011
type: Component
persistent identity: component1903706
display id: component1903706
version: 1
top level: BBa_J45220
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_J45014
type: Component
persistent identity: component1903718
display id: component1903718
version: 1
top level: BBa_J45220
access: public
definition: https://synbiohub.org/public/igem/BBa_J45014/1

BBa_B0032
type: Component
persistent identity: component1903712
display id: component1903712
version: 1
top level: BBa_J45220
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1903721
display id: annotation1903721
version: 1
top level: BBa_J45220
location: https://synbiohub.org/public/igem/BBa_J45220/annotation1903721/range1903721/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J45220/annotation1903721/range1903721/1
type: Range
persistent identity: range1903721
display id: range1903721
version: 1
direction: direction/forward
top level: BBa_J45220
start: 1760
end: 1800
orientation: inline

BBa_J45014
type: SequenceAnnotation
persistent identity: annotation1903718
display id: annotation1903718
version: 1
top level: BBa_J45220
location: https://synbiohub.org/public/igem/BBa_J45220/annotation1903718/range1903718/1
component: BBa_J45014

https://synbiohub.org/public/igem/BBa_J45220/annotation1903718/range1903718/1
type: Range
persistent identity: range1903718
display id: range1903718
version: 1
direction: direction/reverse
top level: BBa_J45220
start: 83
end: 1663
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1903712
display id: annotation1903712
version: 1
top level: BBa_J45220
location: https://synbiohub.org/public/igem/BBa_J45220/annotation1903712/range1903712/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_J45220/annotation1903712/range1903712/1
type: Range
persistent identity: range1903712
display id: range1903712
version: 1
direction: direction/forward
top level: BBa_J45220
start: 64
end: 76
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1903719
display id: annotation1903719
version: 1
top level: BBa_J45220
location: https://synbiohub.org/public/igem/BBa_J45220/annotation1903719/range1903719/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J45220/annotation1903719/range1903719/1
type: Range
persistent identity: range1903719
display id: range1903719
version: 1
direction: direction/forward
top level: BBa_J45220
start: 1672
end: 1751
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1903706
display id: annotation1903706
version: 1
top level: BBa_J45220
location: https://synbiohub.org/public/igem/BBa_J45220/annotation1903706/range1903706/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_J45220/annotation1903706/range1903706/1
type: Range
persistent identity: range1903706
display id: range1903706
version: 1
direction: direction/forward
top level: BBa_J45220
start: 1
end: 54
orientation: inline

BBa_I754001
type: ComponentDefinition
persistent identity: BBa I754001
display id: BBa_I754001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ech protein generator for vanillin biosynthesis
creator: Mahinur Akkaya
created: 2007-06-26T11:00:00Z
modified: 2015-08-31T04:08:06Z
discontinued: false
dominant: true
group u list: _154_
m user id: 0
owner id: 1685
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: for vanillin biosynthesis ech
mutable notes: needs to be used in conjuction with fcs in order to generate vanillin.
mutable provenance: uses "partinfo" BBa_l75400 as well as a promoter, RBS, and terminator. IPTG induceable promoter inordder to test the conctruct. Strong RBS and terminator.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_I754000
component: BBa_R0011
component: BBa_B0012
component: BBa_B0034
component: BBa_B0011
sequence annotation: BBa_I754000
sequence annotation: BBa_B0012
sequence annotation: BBa_R0011
sequence annotation: BBa_B0011
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I754001_sequence/1

BBa_I754000
type: Component
persistent identity: component1935846
display id: component1935846
version: 1
top level: BBa_I754001
access: public
definition: https://synbiohub.org/public/igem/BBa_I754000/1

BBa_R0011
type: Component
persistent identity: component1935838
display id: component1935838
version: 1
top level: BBa_I754001
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_B0012
type: Component
persistent identity: component1935847
display id: component1935847
version: 1
top level: BBa_I754001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component1935844
display id: component1935844
version: 1
top level: BBa_I754001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0011
type: Component
persistent identity: component1935851
display id: component1935851
version: 1
top level: BBa_I754001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0011/1

BBa_I754000
type: SequenceAnnotation
persistent identity: annotation1935846
display id: annotation1935846
version: 1
top level: BBa_I754001
location: https://synbiohub.org/public/igem/BBa_I754001/annotation1935846/range1935846/1
component: BBa_I754000

https://synbiohub.org/public/igem/BBa_I754001/annotation1935846/range1935846/1
type: Range
persistent identity: range1935846
display id: range1935846
version: 1
direction: direction/reverse
top level: BBa_I754001
start: 82
end: 912
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1935847
display id: annotation1935847
version: 1
top level: BBa_I754001
location: https://synbiohub.org/public/igem/BBa_I754001/annotation1935847/range1935847/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I754001/annotation1935847/range1935847/1
type: Range
persistent identity: range1935847
display id: range1935847
version: 1
direction: direction/forward
top level: BBa_I754001
start: 921
end: 961
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1935838
display id: annotation1935838
version: 1
top level: BBa_I754001
location: https://synbiohub.org/public/igem/BBa_I754001/annotation1935838/range1935838/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_I754001/annotation1935838/range1935838/1
type: Range
persistent identity: range1935838
display id: range1935838
version: 1
direction: direction/forward
top level: BBa_I754001
start: 1
end: 54
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation1935851
display id: annotation1935851
version: 1
top level: BBa_I754001
location: https://synbiohub.org/public/igem/BBa_I754001/annotation1935851/range1935851/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_I754001/annotation1935851/range1935851/1
type: Range
persistent identity: range1935851
display id: range1935851
version: 1
direction: direction/forward
top level: BBa_I754001
start: 970
end: 1015
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1935844
display id: annotation1935844
version: 1
top level: BBa_I754001
location: https://synbiohub.org/public/igem/BBa_I754001/annotation1935844/range1935844/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I754001/annotation1935844/range1935844/1
type: Range
persistent identity: range1935844
display id: range1935844
version: 1
direction: direction/reverse
top level: BBa_I754001
start: 64
end: 75
orientation: inline

BBa_K1399023
type: ComponentDefinition
persistent identity: BBa K1399023
display id: BBa_K1399023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ptet-GFP(DAS)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Tetracycline/aTc inducible promoter with GFP reporter tagged with DAS-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.Presence of SspB is crucial for effective protein degradation.
mutable notes: Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
mutable provenance: other biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0034
component: BBa_K1399008
component: BBa_R0040
component: BBa_B0015
sequence annotation: BBa_K1399008
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1399023_sequence/1

BBa_B0034
type: Component
persistent identity: component2384305
display id: component2384305
version: 1
top level: BBa_K1399023
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1399008
type: Component
persistent identity: component2384311
display id: component2384311
version: 1
top level: BBa_K1399023
access: public
definition: https://synbiohub.org/public/igem/BBa_K1399008/1

BBa_R0040
type: Component
persistent identity: component2384299
display id: component2384299
version: 1
top level: BBa_K1399023
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0015
type: Component
persistent identity: component2384318
display id: component2384318
version: 1
top level: BBa_K1399023
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K1399008
type: SequenceAnnotation
persistent identity: annotation2384311
display id: annotation2384311
version: 1
top level: BBa_K1399023
location: https://synbiohub.org/public/igem/BBa_K1399023/annotation2384311/range2384311/1
component: BBa_K1399008

https://synbiohub.org/public/igem/BBa_K1399023/annotation2384311/range2384311/1
type: Range
persistent identity: range2384311
display id: range2384311
version: 1
direction: direction/reverse
top level: BBa_K1399023
start: 81
end: 833
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2384299
display id: annotation2384299
version: 1
top level: BBa_K1399023
location: https://synbiohub.org/public/igem/BBa_K1399023/annotation2384299/range2384299/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K1399023/annotation2384299/range2384299/1
type: Range
persistent identity: range2384299
display id: range2384299
version: 1
direction: direction/forward
top level: BBa_K1399023
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2384305
display id: annotation2384305
version: 1
top level: BBa_K1399023
location: https://synbiohub.org/public/igem/BBa_K1399023/annotation2384305/range2384305/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1399023/annotation2384305/range2384305/1
type: Range
persistent identity: range2384305
display id: range2384305
version: 1
direction: direction/reverse
top level: BBa_K1399023
start: 63
end: 74
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2384318
display id: annotation2384318
version: 1
top level: BBa_K1399023
location: https://synbiohub.org/public/igem/BBa_K1399023/annotation2384318/range2384318/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1399023/annotation2384318/range2384318/1
type: Range
persistent identity: range2384318
display id: range2384318
version: 1
direction: direction/reverse
top level: BBa_K1399023
start: 842
end: 970
orientation: inline