//cds/membrane/transporter
type: Collection
persistent identity: cds membrane transporter
display id: cds_membrane_transporter
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_J07006
member: BBa_K258001
member: BBa_K1404002
member: BBa_K2078001
member: GLF
member: BBa_J92000
member: BBa_K141002
member: BBa_K633001
member: trkD
member: BBa_K141003
member: TRM
member: BBa_K141000
member: BBa_K1067001
member: cmpA
member: BBa_K1404001
member: BBa_K1709005
member: PrtDEF
member: BBa_K1067009
member: BBa_K1172401
member: BBa_J07017
member: BBa_K190028

BBa_K1067001
type: ComponentDefinition
persistent identity: BBa K1067001
display id: BBa_K1067001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Twin-arginine translocation reporter
creator: Kristian Davidsen
created: 2013-09-18T11:00:00Z
modified: 2015-05-08T01:09:00Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1376_
m user id: 0
owner id: 16028
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: bbbb
mutable notes: rrrr
mutable provenance: eeee
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Gap
sequence annotation: start
sequence annotation: rbs
sequence annotation: GFP SF
sequence annotation: TAT signal peptide
sequence annotation: RFP mCherry
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K1067001_sequence/1

Gap
type: SequenceAnnotation
persistent identity: annotation2359399
display id: annotation2359399
version: 1
top level: BBa_K1067001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1067001/annotation2359399/range2359399/1

https://synbiohub.org/public/igem/BBa_K1067001/annotation2359399/range2359399/1
type: Range
persistent identity: range2359399
display id: range2359399
version: 1
direction: direction/forward
top level: BBa_K1067001
start: 854
end: 873
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2359401
display id: annotation2359401
version: 1
top level: BBa_K1067001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1067001/annotation2359401/range2359401/1

https://synbiohub.org/public/igem/BBa_K1067001/annotation2359401/range2359401/1
type: Range
persistent identity: range2359401
display id: range2359401
version: 1
direction: direction/forward
top level: BBa_K1067001
start: 23
end: 25
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2359400
display id: annotation2359400
version: 1
top level: BBa_K1067001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1067001/annotation2359400/range2359400/1

https://synbiohub.org/public/igem/BBa_K1067001/annotation2359400/range2359400/1
type: Range
persistent identity: range2359400
display id: range2359400
version: 1
direction: direction/forward
top level: BBa_K1067001
start: 862
end: 866
orientation: inline

GFP SF
type: SequenceAnnotation
persistent identity: annotation2359398
display id: annotation2359398
version: 1
top level: BBa_K1067001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1067001/annotation2359398/range2359398/1

https://synbiohub.org/public/igem/BBa_K1067001/annotation2359398/range2359398/1
type: Range
persistent identity: range2359398
display id: range2359398
version: 1
direction: direction/forward
top level: BBa_K1067001
start: 140
end: 853
orientation: inline

TAT signal peptide
type: SequenceAnnotation
persistent identity: annotation2359397
display id: annotation2359397
version: 1
top level: BBa_K1067001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1067001/annotation2359397/range2359397/1

https://synbiohub.org/public/igem/BBa_K1067001/annotation2359397/range2359397/1
type: Range
persistent identity: range2359397
display id: range2359397
version: 1
direction: direction/forward
top level: BBa_K1067001
start: 23
end: 139
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2359404
display id: annotation2359404
version: 1
top level: BBa_K1067001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1067001/annotation2359404/range2359404/1

https://synbiohub.org/public/igem/BBa_K1067001/annotation2359404/range2359404/1
type: Range
persistent identity: range2359404
display id: range2359404
version: 1
direction: direction/forward
top level: BBa_K1067001
start: 874
end: 876
orientation: inline

RFP mCherry
type: SequenceAnnotation
persistent identity: annotation2359407
display id: annotation2359407
version: 1
top level: BBa_K1067001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1067001/annotation2359407/range2359407/1

https://synbiohub.org/public/igem/BBa_K1067001/annotation2359407/range2359407/1
type: Range
persistent identity: range2359407
display id: range2359407
version: 1
direction: direction/forward
top level: BBa_K1067001
start: 874
end: 1584
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2359406
display id: annotation2359406
version: 1
top level: BBa_K1067001
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1067001/annotation2359406/range2359406/1

https://synbiohub.org/public/igem/BBa_K1067001/annotation2359406/range2359406/1
type: Range
persistent identity: range2359406
display id: range2359406
version: 1
direction: direction/forward
top level: BBa_K1067001
start: 1582
end: 1584
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2359402
display id: annotation2359402
version: 1
top level: BBa_K1067001
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1067001/annotation2359402/range2359402/1

https://synbiohub.org/public/igem/BBa_K1067001/annotation2359402/range2359402/1
type: Range
persistent identity: range2359402
display id: range2359402
version: 1
direction: direction/forward
top level: BBa_K1067001
start: 851
end: 853
orientation: inline

cmpA
type: ComponentDefinition
persistent identity: BBa K2078000
display id: BBa_K2078000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cmpA
creator: Michael O
created: 2016-10-05T11:00:00Z
modified: 2016-10-06T01:45:19Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2546_
m user id: 30604
owner id: 30629
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Bi-carbonate membrane transporter
mutable notes: -Part was synthesized to already contain RBS. -Scar between RBS and ORF is TACTAG. -The part was synthesized with with BioBrick prefix and suffix. -EcoR1 site was preceded by CCG to allow us to clone the part into pSB1C3. -
mutable provenance: De-novo synthesis by IDT.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: TACTAG
sequence annotation: cmpA
sequence annotation: RBS from Synechocystis 6803
sequence: https://synbiohub.org/public/igem/BBa_K2078000_sequence/1

TACTAG
type: SequenceAnnotation
persistent identity: annotation2486701
display id: annotation2486701
version: 1
top level: cmpA
role: feature/scar
location: https://synbiohub.org/public/igem/BBa_K2078000/annotation2486701/range2486701/1

https://synbiohub.org/public/igem/BBa_K2078000/annotation2486701/range2486701/1
type: Range
persistent identity: range2486701
display id: range2486701
version: 1
direction: direction/forward
top level: cmpA
start: 11
end: 16
orientation: inline

cmpA
type: SequenceAnnotation
persistent identity: annotation2486699
display id: annotation2486699
version: 1
top level: cmpA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K2078000/annotation2486699/range2486699/1

https://synbiohub.org/public/igem/BBa_K2078000/annotation2486699/range2486699/1
type: Range
persistent identity: range2486699
display id: range2486699
version: 1
direction: direction/forward
top level: cmpA
start: 17
end: 1375
orientation: inline

RBS from Synechocystis 6803
type: SequenceAnnotation
persistent identity: annotation2486696
display id: annotation2486696
version: 1
top level: cmpA
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K2078000/annotation2486696/range2486696/1

https://synbiohub.org/public/igem/BBa_K2078000/annotation2486696/range2486696/1
type: Range
persistent identity: range2486696
display id: range2486696
version: 1
direction: direction/forward
top level: cmpA
start: 1
end: 10
orientation: inline

PrtDEF
type: ComponentDefinition
persistent identity: BBa K258008
display id: BBa_K258008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Export of recombinant proteins in Escherichia coli using ABC transporter of Erwinia chrysanthemi
creator: Jung Hoon Ahn from Korea Advanced Institute of Science and Technology
created: 2009-10-09T11:00:00Z
modified: 2015-05-08T01:11:42Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _358_
m user id: 0
owner id: 4260
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: EGF-TliA, EGF-LARD1 fusion proteins were secreted when ABC transporter was present. E. coli harboring the PrtDEF of E. chrysanthemi secreted more fusion proteins than without ABC transporter. In fact, in enzyme activation assay(with Hitachi Spectrophotometer) we observed that although our protein -EGF- has disulfide bonds, it was excreted succesfully. In addition, when compearing with lack of ABC domain, we saw that protein excretion into medium (supernatant) is 10-fold more with ABC transporter-PrtDEF-. PrtDEF functioned well at 37??C, the optimum growth temperature for E. coli
mutable notes: We used whole plasmid in order to excrete a fusion protein with one of two signal tags which are TliA and LARD1
mutable provenance: Sequence and Features PrtDEF: ABC transporter of Erwinia chrysanthemi for PrtA EGF: Human Epidermal Growth Factor
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
component: BBa_K258100
component: BBa_B0010
component: BBa_K258102
component: BBa_B0034
component: BBa_B0012
component: BBa_R0040
component: BBa_K258101
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_K258102
sequence annotation: BBa_K258101
sequence annotation: BBa_R0040
sequence annotation: BBa_K258100
sequence: https://synbiohub.org/public/igem/BBa_K258008_sequence/1

BBa_K258100
type: Component
persistent identity: component2033394
display id: component2033394
version: 1
top level: PrtDEF
access: public
definition: https://synbiohub.org/public/igem/BBa_K258100/1

BBa_B0010
type: Component
persistent identity: component2033405
display id: component2033405
version: 1
top level: PrtDEF
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_K258102
type: Component
persistent identity: component2033404
display id: component2033404
version: 1
top level: PrtDEF
access: public
definition: https://synbiohub.org/public/igem/BBa_K258102/1

BBa_B0010
type: Component
persistent identity: component2033395
display id: component2033395
version: 1
top level: PrtDEF
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component2033403
display id: component2033403
version: 1
top level: PrtDEF
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component2033398
display id: component2033398
version: 1
top level: PrtDEF
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component2033400
display id: component2033400
version: 1
top level: PrtDEF
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component2033407
display id: component2033407
version: 1
top level: PrtDEF
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0040
type: Component
persistent identity: component2033387
display id: component2033387
version: 1
top level: PrtDEF
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0034
type: Component
persistent identity: component2033393
display id: component2033393
version: 1
top level: PrtDEF
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K258101
type: Component
persistent identity: component2033399
display id: component2033399
version: 1
top level: PrtDEF
access: public
definition: https://synbiohub.org/public/igem/BBa_K258101/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2033395
display id: annotation2033395
version: 1
top level: PrtDEF
location: https://synbiohub.org/public/igem/BBa_K258008/annotation2033395/range2033395/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K258008/annotation2033395/range2033395/1
type: Range
persistent identity: range2033395
display id: range2033395
version: 1
direction: direction/forward
top level: PrtDEF
start: 1814
end: 1893
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2033393
display id: annotation2033393
version: 1
top level: PrtDEF
location: https://synbiohub.org/public/igem/BBa_K258008/annotation2033393/range2033393/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K258008/annotation2033393/range2033393/1
type: Range
persistent identity: range2033393
display id: range2033393
version: 1
direction: direction/reverse
top level: PrtDEF
start: 63
end: 74
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2033398
display id: annotation2033398
version: 1
top level: PrtDEF
location: https://synbiohub.org/public/igem/BBa_K258008/annotation2033398/range2033398/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K258008/annotation2033398/range2033398/1
type: Range
persistent identity: range2033398
display id: range2033398
version: 1
direction: direction/reverse
top level: PrtDEF
start: 1902
end: 1913
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2033400
display id: annotation2033400
version: 1
top level: PrtDEF
location: https://synbiohub.org/public/igem/BBa_K258008/annotation2033400/range2033400/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K258008/annotation2033400/range2033400/1
type: Range
persistent identity: range2033400
display id: range2033400
version: 1
direction: direction/forward
top level: PrtDEF
start: 3272
end: 3351
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2033407
display id: annotation2033407
version: 1
top level: PrtDEF
location: https://synbiohub.org/public/igem/BBa_K258008/annotation2033407/range2033407/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K258008/annotation2033407/range2033407/1
type: Range
persistent identity: range2033407
display id: range2033407
version: 1
direction: direction/forward
top level: PrtDEF
start: 4860
end: 4900
orientation: inline

BBa_K258102
type: SequenceAnnotation
persistent identity: annotation2033404
display id: annotation2033404
version: 1
top level: PrtDEF
location: https://synbiohub.org/public/igem/BBa_K258008/annotation2033404/range2033404/1
component: BBa_K258102

https://synbiohub.org/public/igem/BBa_K258008/annotation2033404/range2033404/1
type: Range
persistent identity: range2033404
display id: range2033404
version: 1
direction: direction/reverse
top level: PrtDEF
start: 3378
end: 4763
orientation: inline

BBa_K258101
type: SequenceAnnotation
persistent identity: annotation2033399
display id: annotation2033399
version: 1
top level: PrtDEF
location: https://synbiohub.org/public/igem/BBa_K258008/annotation2033399/range2033399/1
component: BBa_K258101

https://synbiohub.org/public/igem/BBa_K258008/annotation2033399/range2033399/1
type: Range
persistent identity: range2033399
display id: range2033399
version: 1
direction: direction/reverse
top level: PrtDEF
start: 1920
end: 3263
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2033387
display id: annotation2033387
version: 1
top level: PrtDEF
location: https://synbiohub.org/public/igem/BBa_K258008/annotation2033387/range2033387/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K258008/annotation2033387/range2033387/1
type: Range
persistent identity: range2033387
display id: range2033387
version: 1
direction: direction/forward
top level: PrtDEF
start: 1
end: 54
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2033405
display id: annotation2033405
version: 1
top level: PrtDEF
location: https://synbiohub.org/public/igem/BBa_K258008/annotation2033405/range2033405/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K258008/annotation2033405/range2033405/1
type: Range
persistent identity: range2033405
display id: range2033405
version: 1
direction: direction/forward
top level: PrtDEF
start: 4772
end: 4851
orientation: inline

BBa_K258100
type: SequenceAnnotation
persistent identity: annotation2033394
display id: annotation2033394
version: 1
top level: PrtDEF
location: https://synbiohub.org/public/igem/BBa_K258008/annotation2033394/range2033394/1
component: BBa_K258100

https://synbiohub.org/public/igem/BBa_K258008/annotation2033394/range2033394/1
type: Range
persistent identity: range2033394
display id: range2033394
version: 1
direction: direction/reverse
top level: PrtDEF
start: 81
end: 1805
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2033403
display id: annotation2033403
version: 1
top level: PrtDEF
location: https://synbiohub.org/public/igem/BBa_K258008/annotation2033403/range2033403/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K258008/annotation2033403/range2033403/1
type: Range
persistent identity: range2033403
display id: range2033403
version: 1
direction: direction/reverse
top level: PrtDEF
start: 3360
end: 3371
orientation: inline

BBa_J07017
type: ComponentDefinition
persistent identity: BBa J07017
display id: BBa_J07017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FecA protein
creator: Annie
created: 2005-07-11T11:00:00Z
modified: 2015-08-31T04:08:19Z
discontinued: false
dominant: false
experience: experience/None
group u list: _21_
m user id: 0
owner id: 353
owning group id: 21
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: E.coli K-12 coding region for FecA protein
mutable provenance: MG1655
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_J07017_sequence/1

GLF
type: ComponentDefinition
persistent identity: BBa K558005
display id: BBa_K558005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Zymomonas mobilis glucose facilitator gene
creator: Vadim Gladwill, Laura Colgin
created: 2011-09-19T11:00:00Z
modified: 2015-05-08T01:12:41Z
discontinued: false
dominant: false
experience: experience/None
group u list: _726_
m user id: 0
owner id: 6570
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
sequence annotation: RBS
sequence annotation: stop
sequence annotation: BBa_B0014 Double Terminator
sequence annotation: GLF
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K558005_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2145000
display id: annotation2145000
version: 1
top level: GLF
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K558005/annotation2145000/range2145000/1

https://synbiohub.org/public/igem/BBa_K558005/annotation2145000/range2145000/1
type: Range
persistent identity: range2145000
display id: range2145000
version: 1
direction: direction/forward
top level: GLF
start: 1
end: 17
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2145031
display id: annotation2145031
version: 1
top level: GLF
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K558005/annotation2145031/range2145031/1

https://synbiohub.org/public/igem/BBa_K558005/annotation2145031/range2145031/1
type: Range
persistent identity: range2145031
display id: range2145031
version: 1
direction: direction/forward
top level: GLF
start: 1464
end: 1466
orientation: inline

BBa_B0014 Double Terminator
type: SequenceAnnotation
persistent identity: annotation2145002
display id: annotation2145002
version: 1
top level: GLF
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K558005/annotation2145002/range2145002/1

https://synbiohub.org/public/igem/BBa_K558005/annotation2145002/range2145002/1
type: Range
persistent identity: range2145002
display id: range2145002
version: 1
direction: direction/forward
top level: GLF
start: 1469
end: 1564
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2145032
display id: annotation2145032
version: 1
top level: GLF
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K558005/annotation2145032/range2145032/1

https://synbiohub.org/public/igem/BBa_K558005/annotation2145032/range2145032/1
type: Range
persistent identity: range2145032
display id: range2145032
version: 1
direction: direction/forward
top level: GLF
start: 1467
end: 1469
orientation: inline

GLF
type: SequenceAnnotation
persistent identity: annotation2145001
display id: annotation2145001
version: 1
top level: GLF
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K558005/annotation2145001/range2145001/1

https://synbiohub.org/public/igem/BBa_K558005/annotation2145001/range2145001/1
type: Range
persistent identity: range2145001
display id: range2145001
version: 1
direction: direction/forward
top level: GLF
start: 18
end: 1468
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2145030
display id: annotation2145030
version: 1
top level: GLF
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K558005/annotation2145030/range2145030/1

https://synbiohub.org/public/igem/BBa_K558005/annotation2145030/range2145030/1
type: Range
persistent identity: range2145030
display id: range2145030
version: 1
direction: direction/forward
top level: GLF
start: 18
end: 20
orientation: inline

BBa_K141003
type: ComponentDefinition
persistent identity: BBa K141003
display id: BBa_K141003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ucp 76 deleted
creator: Jaume For??s Martos
created: 2008-10-05T11:00:00Z
modified: 2015-05-08T01:10:18Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _229_
m user id: 0
owner id: 2947
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: none
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K141003_sequence/1

BBa_J92000
type: ComponentDefinition
persistent identity: BBa J92000
display id: BBa_J92000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lead Uptake Transporter
creator: Justin D Smith
created: 2010-05-22T11:00:00Z
modified: 2015-05-08T01:08:30Z
discontinued: false
dominant: false
group u list: _415_
m user id: 0
owner id: 6514
owning group id: 415
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part was derived from the Pbr T lead uptake protein from Ralstonia metallidurans. The protein is responsible for the uptake of Pb 2+ ions. This part can be used for projects where one needs to uptake lead ions from the environment into the cell interior.
mutable notes: When designing the part we checked the gene sequence/restriction site profile to make sure the part would be compatible with standards 10 and 23.
mutable provenance: Borremans,B., Hobman,J.L., Provoost,A., Brown,N.L. and van Der Lelie,D. "Cloning and functional analysis of the pbr lead resistance determinant of Ralstonia metallidurans CH34" J. Bacteriol. 183 (19), 5651-5658 (2001)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_J92000_sequence/1

BBa_K1067009
type: ComponentDefinition
persistent identity: BBa K1067009
display id: BBa_K1067009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Periplasm transport visualization device
creator: Kristian Davidsen
created: 2013-09-27T11:00:00Z
modified: 2015-05-08T01:09:00Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1376_
m user id: 0
owner id: 16028
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: ggg
mutable notes: ggg
mutable provenance: ggg
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: stop
sequence annotation: araO1R
sequence annotation: GFP SF
sequence annotation: -10
sequence annotation: Tight pBAD promoter
sequence annotation: araC
sequence annotation: start
sequence annotation: CRP binding site
sequence annotation: araI1
sequence annotation: rbs
sequence annotation: -35
sequence annotation: araI2
sequence annotation: BBa_K1067001
sequence annotation: araO1L
sequence annotation: araO2
sequence annotation: TAT signal peptide
sequence annotation: BBa_K1067007
sequence annotation: RFP mCherry
sequence annotation: Gap
sequence: https://synbiohub.org/public/igem/BBa_K1067009_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2366517
display id: annotation2366517
version: 1
top level: BBa_K1067009
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366517/range2366517/1

https://synbiohub.org/public/igem/BBa_K1067009/annotation2366517/range2366517/1
type: Range
persistent identity: range2366517
display id: range2366517
version: 1
direction: direction/forward
top level: BBa_K1067009
start: 2068
end: 2070
orientation: inline

araO1R
type: SequenceAnnotation
persistent identity: annotation2366506
display id: annotation2366506
version: 1
top level: BBa_K1067009
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366506/range2366506/1

https://synbiohub.org/public/igem/BBa_K1067009/annotation2366506/range2366506/1
type: Range
persistent identity: range2366506
display id: range2366506
version: 1
direction: direction/forward
top level: BBa_K1067009
start: 1066
end: 1082
orientation: inline

GFP SF
type: SequenceAnnotation
persistent identity: annotation2366516
display id: annotation2366516
version: 1
top level: BBa_K1067009
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366516/range2366516/1

https://synbiohub.org/public/igem/BBa_K1067009/annotation2366516/range2366516/1
type: Range
persistent identity: range2366516
display id: range2366516
version: 1
direction: direction/forward
top level: BBa_K1067009
start: 1357
end: 2070
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2366512
display id: annotation2366512
version: 1
top level: BBa_K1067009
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366512/range2366512/1

https://synbiohub.org/public/igem/BBa_K1067009/annotation2366512/range2366512/1
type: Range
persistent identity: range2366512
display id: range2366512
version: 1
direction: direction/forward
top level: BBa_K1067009
start: 1177
end: 1182
orientation: inline

Tight pBAD promoter
type: SequenceAnnotation
persistent identity: annotation2366510
display id: annotation2366510
version: 1
top level: BBa_K1067009
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366510/range2366510/1

https://synbiohub.org/public/igem/BBa_K1067009/annotation2366510/range2366510/1
type: Range
persistent identity: range2366510
display id: range2366510
version: 1
direction: direction/forward
top level: BBa_K1067009
start: 1153
end: 1189
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation2366503
display id: annotation2366503
version: 1
top level: BBa_K1067009
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366503/range2366503/1

https://synbiohub.org/public/igem/BBa_K1067009/annotation2366503/range2366503/1
type: Range
persistent identity: range2366503
display id: range2366503
version: 1
direction: direction/forward
top level: BBa_K1067009
start: 1
end: 880
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2366515
display id: annotation2366515
version: 1
top level: BBa_K1067009
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366515/range2366515/1

https://synbiohub.org/public/igem/BBa_K1067009/annotation2366515/range2366515/1
type: Range
persistent identity: range2366515
display id: range2366515
version: 1
direction: direction/forward
top level: BBa_K1067009
start: 1240
end: 1242
orientation: inline

CRP binding site
type: SequenceAnnotation
persistent identity: annotation2366507
display id: annotation2366507
version: 1
top level: BBa_K1067009
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366507/range2366507/1

https://synbiohub.org/public/igem/BBa_K1067009/annotation2366507/range2366507/1
type: Range
persistent identity: range2366507
display id: range2366507
version: 1
direction: direction/forward
top level: BBa_K1067009
start: 1087
end: 1108
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2366522
display id: annotation2366522
version: 1
top level: BBa_K1067009
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366522/range2366522/1

https://synbiohub.org/public/igem/BBa_K1067009/annotation2366522/range2366522/1
type: Range
persistent identity: range2366522
display id: range2366522
version: 1
direction: direction/forward
top level: BBa_K1067009
start: 2799
end: 2801
orientation: inline

araI1
type: SequenceAnnotation
persistent identity: annotation2366508
display id: annotation2366508
version: 1
top level: BBa_K1067009
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366508/range2366508/1

https://synbiohub.org/public/igem/BBa_K1067009/annotation2366508/range2366508/1
type: Range
persistent identity: range2366508
display id: range2366508
version: 1
direction: direction/forward
top level: BBa_K1067009
start: 1119
end: 1135
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2366519
display id: annotation2366519
version: 1
top level: BBa_K1067009
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366519/range2366519/1

https://synbiohub.org/public/igem/BBa_K1067009/annotation2366519/range2366519/1
type: Range
persistent identity: range2366519
display id: range2366519
version: 1
direction: direction/forward
top level: BBa_K1067009
start: 2079
end: 2083
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2366511
display id: annotation2366511
version: 1
top level: BBa_K1067009
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366511/range2366511/1

https://synbiohub.org/public/igem/BBa_K1067009/annotation2366511/range2366511/1
type: Range
persistent identity: range2366511
display id: range2366511
version: 1
direction: direction/forward
top level: BBa_K1067009
start: 1153
end: 1158
orientation: inline

araI2
type: SequenceAnnotation
persistent identity: annotation2366509
display id: annotation2366509
version: 1
top level: BBa_K1067009
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366509/range2366509/1

https://synbiohub.org/public/igem/BBa_K1067009/annotation2366509/range2366509/1
type: Range
persistent identity: range2366509
display id: range2366509
version: 1
direction: direction/forward
top level: BBa_K1067009
start: 1140
end: 1157
orientation: inline

BBa_K1067001
type: SequenceAnnotation
persistent identity: annotation2366523
display id: annotation2366523
version: 1
top level: BBa_K1067009
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366523/range2366523/1

https://synbiohub.org/public/igem/BBa_K1067009/annotation2366523/range2366523/1
type: Range
persistent identity: range2366523
display id: range2366523
version: 1
direction: direction/reverse
top level: BBa_K1067009
start: 1218
end: 2801
orientation: inline

araO1L
type: SequenceAnnotation
persistent identity: annotation2366505
display id: annotation2366505
version: 1
top level: BBa_K1067009
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366505/range2366505/1

https://synbiohub.org/public/igem/BBa_K1067009/annotation2366505/range2366505/1
type: Range
persistent identity: range2366505
display id: range2366505
version: 1
direction: direction/forward
top level: BBa_K1067009
start: 1045
end: 1061
orientation: inline

araO2
type: SequenceAnnotation
persistent identity: annotation2366504
display id: annotation2366504
version: 1
top level: BBa_K1067009
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366504/range2366504/1

https://synbiohub.org/public/igem/BBa_K1067009/annotation2366504/range2366504/1
type: Range
persistent identity: range2366504
display id: range2366504
version: 1
direction: direction/forward
top level: BBa_K1067009
start: 908
end: 924
orientation: inline

TAT signal peptide
type: SequenceAnnotation
persistent identity: annotation2366514
display id: annotation2366514
version: 1
top level: BBa_K1067009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366514/range2366514/1

https://synbiohub.org/public/igem/BBa_K1067009/annotation2366514/range2366514/1
type: Range
persistent identity: range2366514
display id: range2366514
version: 1
direction: direction/forward
top level: BBa_K1067009
start: 1240
end: 1356
orientation: inline

BBa_K1067007
type: SequenceAnnotation
persistent identity: annotation2366513
display id: annotation2366513
version: 1
top level: BBa_K1067009
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366513/range2366513/1

https://synbiohub.org/public/igem/BBa_K1067009/annotation2366513/range2366513/1
type: Range
persistent identity: range2366513
display id: range2366513
version: 1
direction: direction/reverse
top level: BBa_K1067009
start: 1
end: 1209
orientation: inline

RFP mCherry
type: SequenceAnnotation
persistent identity: annotation2366521
display id: annotation2366521
version: 1
top level: BBa_K1067009
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366521/range2366521/1

https://synbiohub.org/public/igem/BBa_K1067009/annotation2366521/range2366521/1
type: Range
persistent identity: range2366521
display id: range2366521
version: 1
direction: direction/forward
top level: BBa_K1067009
start: 2091
end: 2801
orientation: inline

Gap
type: SequenceAnnotation
persistent identity: annotation2366518
display id: annotation2366518
version: 1
top level: BBa_K1067009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366518/range2366518/1

https://synbiohub.org/public/igem/BBa_K1067009/annotation2366518/range2366518/1
type: Range
persistent identity: range2366518
display id: range2366518
version: 1
direction: direction/forward
top level: BBa_K1067009
start: 2071
end: 2090
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2366520
display id: annotation2366520
version: 1
top level: BBa_K1067009
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366520/range2366520/1

https://synbiohub.org/public/igem/BBa_K1067009/annotation2366520/range2366520/1
type: Range
persistent identity: range2366520
display id: range2366520
version: 1
direction: direction/forward
top level: BBa_K1067009
start: 2091
end: 2093
orientation: inline

TRM
type: ComponentDefinition
persistent identity: BBa K1145001
display id: BBa_K1145001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: A transporter which is involved in transporting atrazine.
creator: KUN LV
created: 2013-09-12T11:00:00Z
modified: 2015-05-08T01:09:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1457_
m user id: 0
owner id: 16641
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: TRM is a trans-membrane transporter which involves in transporting atrazine.
mutable notes: No.
mutable provenance: It comes from the genome of Acinetobacter baumannii.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1145001_sequence/1

BBa_K1709005
type: ComponentDefinition
persistent identity: BBa K1709005
display id: BBa_K1709005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ag43-YFP
creator: Astrid Deryckere
created: 2015-09-17T11:00:00Z
modified: 2015-09-18T04:44:46Z
discontinued: false
dominant: false
group u list: _2129_
m user id: 25389
owner id: 25389
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Antigen 43 (Ag43) is the product of the flu gene and works as a cell-surface autotransporter protein found in Escherichia coli. It is expressed to induce cell-cell aggregation and is involved in biofilm formation.
mutable notes: We made a fusion protein of Ag43 and YFP to visualise the clumps of cells
mutable provenance: E. Coli genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ATG Start Codon
sequence annotation: Ag43
sequence annotation: Ochre Stop Codon
sequence annotation: YFP
sequence: https://synbiohub.org/public/igem/BBa_K1709005_sequence/1

ATG Start Codon
type: SequenceAnnotation
persistent identity: annotation2470929
display id: annotation2470929
version: 1
top level: BBa_K1709005
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1709005/annotation2470929/range2470929/1

https://synbiohub.org/public/igem/BBa_K1709005/annotation2470929/range2470929/1
type: Range
persistent identity: range2470929
display id: range2470929
version: 1
direction: direction/forward
top level: BBa_K1709005
start: 1
end: 3
orientation: inline

Ag43
type: SequenceAnnotation
persistent identity: annotation2470932
display id: annotation2470932
version: 1
top level: BBa_K1709005
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1709005/annotation2470932/range2470932/1

https://synbiohub.org/public/igem/BBa_K1709005/annotation2470932/range2470932/1
type: Range
persistent identity: range2470932
display id: range2470932
version: 1
direction: direction/forward
top level: BBa_K1709005
start: 1
end: 3116
orientation: inline

Ochre Stop Codon
type: SequenceAnnotation
persistent identity: annotation2470930
display id: annotation2470930
version: 1
top level: BBa_K1709005
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1709005/annotation2470930/range2470930/1

https://synbiohub.org/public/igem/BBa_K1709005/annotation2470930/range2470930/1
type: Range
persistent identity: range2470930
display id: range2470930
version: 1
direction: direction/forward
top level: BBa_K1709005
start: 3841
end: 3843
orientation: inline

YFP
type: SequenceAnnotation
persistent identity: annotation2470931
display id: annotation2470931
version: 1
top level: BBa_K1709005
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1709005/annotation2470931/range2470931/1

https://synbiohub.org/public/igem/BBa_K1709005/annotation2470931/range2470931/1
type: Range
persistent identity: range2470931
display id: range2470931
version: 1
direction: direction/forward
top level: BBa_K1709005
start: 3120
end: 3843
orientation: inline

BBa_K190028
type: ComponentDefinition
persistent identity: BBa K190028
display id: BBa_K190028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GlpF
creator: Jolanda Witteveen
created: 2009-08-25T11:00:00Z
modified: 2015-05-08T01:11:15Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _306_
m user id: 0
owner id: 4226
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: GlpF is an aquaglyceroporin channel that facilitates the transport of As(III).
mutable notes: removal of an EcoR1 restiction site
mutable provenance: This part comes from genomic DNA of the Escherichia coli 536.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GlpF
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K190028_sequence/1

GlpF
type: SequenceAnnotation
persistent identity: annotation2061212
display id: annotation2061212
version: 1
top level: BBa_K190028
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K190028/annotation2061212/range2061212/1

https://synbiohub.org/public/igem/BBa_K190028/annotation2061212/range2061212/1
type: Range
persistent identity: range2061212
display id: range2061212
version: 1
direction: direction/forward
top level: BBa_K190028
start: 1
end: 846
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2018038
display id: annotation2018038
version: 1
top level: BBa_K190028
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K190028/annotation2018038/range2018038/1

https://synbiohub.org/public/igem/BBa_K190028/annotation2018038/range2018038/1
type: Range
persistent identity: range2018038
display id: range2018038
version: 1
direction: direction/forward
top level: BBa_K190028
start: 1
end: 3
orientation: inline

BBa_K1404001
type: ComponentDefinition
persistent identity: BBa K1404001
display id: BBa_K1404001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ptac-NiCoTB-rcn-csgBAEFG, turns E. coli into a functional Ni and Co biofilter
creator: Alexandre Duprey
created: 2014-09-22T11:00:00Z
modified: 2015-05-08T01:10:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1782_
m user id: 0
owner id: 8419
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part, when expressed in a rcnA E. coli strain, allows the formation of a functional Ni2+ and Co2+ biofilter capturing up to 3 mg of metal per g of bacterial dry weight when exposed to 20 ??M Ni2+ or Co2+, in only 10 mn. This is one of the best performances and the fastest reported until now.
mutable notes: Temp
mutable provenance: [lien] and [lien] were cloned by 3A assembly.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1404002
component: BBa_K540000
sequence annotation: BBa_K1404002
sequence annotation: BBa_K540000
sequence: https://synbiohub.org/public/igem/BBa_K1404001_sequence/1

BBa_K1404002
type: Component
persistent identity: component2385413
display id: component2385413
version: 1
top level: BBa_K1404001
access: public
definition: BBa_K1404002

BBa_K540000
type: Component
persistent identity: component2385428
display id: component2385428
version: 1
top level: BBa_K1404001
access: public
definition: https://synbiohub.org/public/igem/BBa_K540000/1

BBa_K1404002
type: SequenceAnnotation
persistent identity: annotation2385413
display id: annotation2385413
version: 1
top level: BBa_K1404001
location: https://synbiohub.org/public/igem/BBa_K1404001/annotation2385413/range2385413/1
component: BBa_K1404002

https://synbiohub.org/public/igem/BBa_K1404001/annotation2385413/range2385413/1
type: Range
persistent identity: range2385413
display id: range2385413
version: 1
direction: direction/reverse
top level: BBa_K1404001
start: 1
end: 1306
orientation: inline

BBa_K540000
type: SequenceAnnotation
persistent identity: annotation2385428
display id: annotation2385428
version: 1
top level: BBa_K1404001
location: https://synbiohub.org/public/igem/BBa_K1404001/annotation2385428/range2385428/1
component: BBa_K540000

https://synbiohub.org/public/igem/BBa_K1404001/annotation2385428/range2385428/1
type: Range
persistent identity: range2385428
display id: range2385428
version: 1
direction: direction/reverse
top level: BBa_K1404001
start: 1315
end: 4437
orientation: inline

BBa_J07006
type: ComponentDefinition
persistent identity: BBa J07006
display id: BBa_J07006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: malE
creator: igem_mit_2005
created: 2005-07-05T11:00:00Z
modified: 2015-08-31T04:08:19Z
discontinued: false
dominant: false
experience: experience/None
group u list: _21_
m user id: 0
owner id: 379
owning group id: 21
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: malE - a monomer. This monomeric protein is used as a negative control when testing for dimerization.
mutable provenance: Genbank E.coli K-12 strain
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: malE
sequence: https://synbiohub.org/public/igem/BBa_J07006_sequence/1

malE
type: SequenceAnnotation
persistent identity: annotation1569704
display id: annotation1569704
version: 1
top level: BBa_J07006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J07006/annotation1569704/range1569704/1

https://synbiohub.org/public/igem/BBa_J07006/annotation1569704/range1569704/1
type: Range
persistent identity: range1569704
display id: range1569704
version: 1
direction: direction/forward
top level: BBa_J07006
start: 1
end: 1191
orientation: inline

BBa_K1404002
type: ComponentDefinition
persistent identity: BBa K1404002
display id: BBa_K1404002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ptac-NiCoTB, improves nickel and cobalt internalization
creator: Alexandre Duprey
created: 2014-09-22T11:00:00Z
modified: 2015-07-23T03:01:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1782_
m user id: 4206
owner id: 8419
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part comprises a strong E. coli promoter with...
mutable notes: Temp
mutable provenance: The promoter and the rest...
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: NiCoT
sequence annotation: Ptac
sequence annotation: B0014 terminator
sequence annotation: RBS Paris 2009
sequence: https://synbiohub.org/public/igem/BBa_K1404002_sequence/1

NiCoT
type: SequenceAnnotation
persistent identity: annotation2385406
display id: annotation2385406
version: 1
top level: BBa_K1404002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1404002/annotation2385406/range2385406/1

https://synbiohub.org/public/igem/BBa_K1404002/annotation2385406/range2385406/1
type: Range
persistent identity: range2385406
display id: range2385406
version: 1
direction: direction/forward
top level: BBa_K1404002
start: 96
end: 1212
orientation: inline

Ptac
type: SequenceAnnotation
persistent identity: annotation2385405
display id: annotation2385405
version: 1
top level: BBa_K1404002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1404002/annotation2385405/range2385405/1

https://synbiohub.org/public/igem/BBa_K1404002/annotation2385405/range2385405/1
type: Range
persistent identity: range2385405
display id: range2385405
version: 1
direction: direction/forward
top level: BBa_K1404002
start: 1
end: 76
orientation: inline

B0014 terminator
type: SequenceAnnotation
persistent identity: annotation2385408
display id: annotation2385408
version: 1
top level: BBa_K1404002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404002/annotation2385408/range2385408/1

https://synbiohub.org/public/igem/BBa_K1404002/annotation2385408/range2385408/1
type: Range
persistent identity: range2385408
display id: range2385408
version: 1
direction: direction/forward
top level: BBa_K1404002
start: 1212
end: 1306
orientation: inline

RBS Paris 2009
type: SequenceAnnotation
persistent identity: annotation2385407
display id: annotation2385407
version: 1
top level: BBa_K1404002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1404002/annotation2385407/range2385407/1

https://synbiohub.org/public/igem/BBa_K1404002/annotation2385407/range2385407/1
type: Range
persistent identity: range2385407
display id: range2385407
version: 1
direction: direction/forward
top level: BBa_K1404002
start: 83
end: 95
orientation: inline

BBa_K141002
type: ComponentDefinition
persistent identity: BBa K141002
display id: BBa_K141002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ucp 175 deleted
creator: Jaume For??s Martos
created: 2008-10-05T11:00:00Z
modified: 2015-05-08T01:10:18Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _229_
m user id: 0
owner id: 2947
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: none
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K141002_sequence/1

trkD
type: ComponentDefinition
persistent identity: BBa K629005
display id: BBa_K629005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: trkD, a functional Kup (formerly TrkD) system took up Cs+ with a moderate rate and affinity
creator: Zilong WANG, Yi ZHENG
created: 2011-10-03T11:00:00Z
modified: 2015-05-08T01:12:56Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _801_
m user id: 0
owner id: 9022
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Escherichia coli cells which contain a functional Kup (formerly TrkD) system took up Cs' with a moderate rate and affinity. Kup is a separate K+ uptake system with relatively little discrimination in the transport of the cations K+, Rb+, and Cs'. Regardless of the presence or absence of Kup, K+-replete cells took up Cs'primarily by a very low affinity mode, proportional to the ratio of the Cs' and K+ concentrations in the medium. In our project, trkD is used to collect Cs-137 when recN is activated by radiation and start the transcription of trkD,in order to absorb this kind of radioactive source like Cs-137
mutable notes: none
mutable provenance: ATGAGCACTGATAATAAGCAATCATTGCCCGCGATTACCCTCGCGGCGATTGGAGTTGTCTACGGCGATATTGGTACCAGCCCGTTATATACACTTCGTGAATGTTTGTCCGGCCAGTTTGGTTTTGGCGTTGAACGCGATGCCGTGTTTGGCTTTTTATCGCTGATCTTCTGGCTGCTAATCTTTGTGGTTTCCATTAAATATCTCACCTTCGTGATGCGGGCAGATAACGCCGGTGAGGGGGGGATCCTGACGTTGATGTCGCTTGCCGGGCGTAATACGTCGGCGCGAACCACATCAATGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAGCATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCGATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGCTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGACACTTTGGTAAGTTCCCTATTCGTCTGGCGTGGTTCACTGTCGTATTACCTTCCTTAACTCTTAATTACTTCGGCCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTTTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATCGCCTCGCAGGCGGTTATCTCTGGCGTCTTTTCATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCGATGCGCATTATTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTATCGTGATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGACCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTGTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TrkD
sequence annotation: terminator
sequence annotation: start
sequence annotation: fwd primer
sequence annotation: rev primer
sequence: https://synbiohub.org/public/igem/BBa_K629005_sequence/1

TrkD
type: SequenceAnnotation
persistent identity: annotation2157756
display id: annotation2157756
version: 1
top level: trkD
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K629005/annotation2157756/range2157756/1

https://synbiohub.org/public/igem/BBa_K629005/annotation2157756/range2157756/1
type: Range
persistent identity: range2157756
display id: range2157756
version: 1
direction: direction/forward
top level: trkD
start: 1
end: 1869
orientation: inline

terminator
type: SequenceAnnotation
persistent identity: annotation2157758
display id: annotation2157758
version: 1
top level: trkD
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K629005/annotation2157758/range2157758/1

https://synbiohub.org/public/igem/BBa_K629005/annotation2157758/range2157758/1
type: Range
persistent identity: range2157758
display id: range2157758
version: 1
direction: direction/forward
top level: trkD
start: 1867
end: 1869
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2157754
display id: annotation2157754
version: 1
top level: trkD
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K629005/annotation2157754/range2157754/1

https://synbiohub.org/public/igem/BBa_K629005/annotation2157754/range2157754/1
type: Range
persistent identity: range2157754
display id: range2157754
version: 1
direction: direction/forward
top level: trkD
start: 1
end: 3
orientation: inline

fwd primer
type: SequenceAnnotation
persistent identity: annotation2157755
display id: annotation2157755
version: 1
top level: trkD
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K629005/annotation2157755/range2157755/1

https://synbiohub.org/public/igem/BBa_K629005/annotation2157755/range2157755/1
type: Range
persistent identity: range2157755
display id: range2157755
version: 1
direction: direction/forward
top level: trkD
start: 1
end: 21
orientation: inline

rev primer
type: SequenceAnnotation
persistent identity: annotation2157757
display id: annotation2157757
version: 1
top level: trkD
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K629005/annotation2157757/range2157757/1

https://synbiohub.org/public/igem/BBa_K629005/annotation2157757/range2157757/1
type: Range
persistent identity: range2157757
display id: range2157757
version: 1
direction: direction/reverse
top level: trkD
start: 1849
end: 1869
orientation: inline

BBa_K258001
type: ComponentDefinition
persistent identity: BBa K258001
display id: BBa_K258001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lipase ABC transporter recognition domain (LARD 1)
creator: Jung Hoon Ahn from Korea Advanced Institute of Science and Technology
created: 2009-10-02T11:00:00Z
modified: 2015-05-08T01:11:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _358_
m user id: 0
owner id: 4260
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Lard 1 was designed for the secretion of fusion proteins.The LARD 1 included four glycine-rich repeats comprising a β-roll structure, and were added to the C-terminus of test proteins. Either Pro-Gly linker or Factor Xa site can be added between fusion proteins and LARD 1. Upon supplementation of E. coli with ABC transporter from E. chrysanthemi (ABC transporter PrtDEF), which function well in the phylogenetically-neighboring genus E.coli, EGF-LARDs were excreted into the culture supernatant.
mutable notes: In our designed LARD 1, we favoured not to add these cleavage site so if you want, yo can add either Pro-Gly linker or Factor Xa site. Secretion of fusion proteins with LARDs could be detected by Western blotting. Fusion proteins were traced in the culture supernatant using antibody against LARDs. Polyclonal antibodies against the C-terminal signal sequence were produced with a synthetic peptide (YQPTDRLVFQGADGST, residues 421???436 of TliA ,also of LARD).
mutable provenance: LARD 1 composed of residues 303-476 of thermostable lipase (TliA) of Pseudomonas fluorescens.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_K258001_sequence/1

BBa_K1172401
type: ComponentDefinition
persistent identity: BBa K1172401
display id: BBa_K1172401
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mtrCAB - gene cluster from S. oneidensis for cytochrome expression
creator: Sebastian Grenz
created: 2013-08-25T11:00:00Z
modified: 2015-05-08T01:09:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1485_
m user id: 0
owner id: 16785
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Minimal set of genes for the expression of working cytochromes. Allows the organism to transfer electrons to its outer membrane.
mutable notes: Two illegal PstI restriction sites, which had to be removed by specific primer extensions.
mutable provenance: Shewanella oneidensis MR-1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: mtrA
sequence annotation: start
sequence annotation: PstI site G->A
sequence annotation: mtrC
sequence annotation: mtrB
sequence annotation: pstI site G->A
sequence: https://synbiohub.org/public/igem/BBa_K1172401_sequence/1

mtrA
type: SequenceAnnotation
persistent identity: annotation2370540
display id: annotation2370540
version: 1
top level: BBa_K1172401
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1172401/annotation2370540/range2370540/1

https://synbiohub.org/public/igem/BBa_K1172401/annotation2370540/range2370540/1
type: Range
persistent identity: range2370540
display id: range2370540
version: 1
direction: direction/forward
top level: BBa_K1172401
start: 2017
end: 3018
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2370544
display id: annotation2370544
version: 1
top level: BBa_K1172401
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1172401/annotation2370544/range2370544/1

https://synbiohub.org/public/igem/BBa_K1172401/annotation2370544/range2370544/1
type: Range
persistent identity: range2370544
display id: range2370544
version: 1
direction: direction/forward
top level: BBa_K1172401
start: 3019
end: 3021
orientation: inline

PstI site G->A
type: SequenceAnnotation
persistent identity: annotation2370539
display id: annotation2370539
version: 1
top level: BBa_K1172401
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K1172401/annotation2370539/range2370539/1

https://synbiohub.org/public/igem/BBa_K1172401/annotation2370539/range2370539/1
type: Range
persistent identity: range2370539
display id: range2370539
version: 1
direction: direction/forward
top level: BBa_K1172401
start: 1798
end: 1798
orientation: inline

mtrC
type: SequenceAnnotation
persistent identity: annotation2370537
display id: annotation2370537
version: 1
top level: BBa_K1172401
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1172401/annotation2370537/range2370537/1

https://synbiohub.org/public/igem/BBa_K1172401/annotation2370537/range2370537/1
type: Range
persistent identity: range2370537
display id: range2370537
version: 1
direction: direction/forward
top level: BBa_K1172401
start: 1
end: 2016
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2370543
display id: annotation2370543
version: 1
top level: BBa_K1172401
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1172401/annotation2370543/range2370543/1

https://synbiohub.org/public/igem/BBa_K1172401/annotation2370543/range2370543/1
type: Range
persistent identity: range2370543
display id: range2370543
version: 1
direction: direction/forward
top level: BBa_K1172401
start: 2017
end: 2019
orientation: inline

mtrB
type: SequenceAnnotation
persistent identity: annotation2370542
display id: annotation2370542
version: 1
top level: BBa_K1172401
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1172401/annotation2370542/range2370542/1

https://synbiohub.org/public/igem/BBa_K1172401/annotation2370542/range2370542/1
type: Range
persistent identity: range2370542
display id: range2370542
version: 1
direction: direction/forward
top level: BBa_K1172401
start: 3019
end: 5112
orientation: inline

pstI site G->A
type: SequenceAnnotation
persistent identity: annotation2370541
display id: annotation2370541
version: 1
top level: BBa_K1172401
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K1172401/annotation2370541/range2370541/1

https://synbiohub.org/public/igem/BBa_K1172401/annotation2370541/range2370541/1
type: Range
persistent identity: range2370541
display id: range2370541
version: 1
direction: direction/forward
top level: BBa_K1172401
start: 2083
end: 2083
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2370538
display id: annotation2370538
version: 1
top level: BBa_K1172401
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1172401/annotation2370538/range2370538/1

https://synbiohub.org/public/igem/BBa_K1172401/annotation2370538/range2370538/1
type: Range
persistent identity: range2370538
display id: range2370538
version: 1
direction: direction/forward
top level: BBa_K1172401
start: 1
end: 6
orientation: inline

BBa_K633001
type: ComponentDefinition
persistent identity: BBa K633001
display id: BBa_K633001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Linker + EstA Membrane Protein
creator: Ricardo Camilo Chavez Martinez
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _807_
m user id: 0
owner id: 9146
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: We used a linker attached to a protein membrane "estA"
mutable notes: None whatsoever
mutable provenance: where does it come from?
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: linker
sequence annotation: estA
sequence: https://synbiohub.org/public/igem/BBa_K633001_sequence/1

linker
type: SequenceAnnotation
persistent identity: annotation2145004
display id: annotation2145004
version: 1
top level: BBa_K633001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K633001/annotation2145004/range2145004/1

https://synbiohub.org/public/igem/BBa_K633001/annotation2145004/range2145004/1
type: Range
persistent identity: range2145004
display id: range2145004
version: 1
direction: direction/forward
top level: BBa_K633001
start: 1
end: 33
orientation: inline

estA
type: SequenceAnnotation
persistent identity: annotation2145003
display id: annotation2145003
version: 1
top level: BBa_K633001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K633001/annotation2145003/range2145003/1

https://synbiohub.org/public/igem/BBa_K633001/annotation2145003/range2145003/1
type: Range
persistent identity: range2145003
display id: range2145003
version: 1
direction: direction/forward
top level: BBa_K633001
start: 34
end: 876
orientation: inline

BBa_K2078001
type: ComponentDefinition
persistent identity: BBa K2078001
display id: BBa_K2078001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23119 + cmpA
creator: Cameron Anderson
created: 2016-10-05T11:00:00Z
modified: 2016-10-06T01:48:43Z
discontinued: false
dominant: false
group u list: _2546_
m user id: 30604
owner id: 30629
owning group id: 9
status: status/Planning
bookmark: false
mutable description: J23119 promoter for Synechocystis 6803 + cmpA.
mutable notes: See K2078000
mutable provenance: J23119 + K2078000
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K2078000
component: BBa_J23119
sequence annotation: BBa_J23119
sequence annotation: BBa_K2078000
sequence: https://synbiohub.org/public/igem/BBa_K2078001_sequence/1

BBa_K2078000
type: Component
persistent identity: component2486719
display id: component2486719
version: 1
top level: BBa_K2078001
access: public
definition: cmpA

BBa_J23119
type: Component
persistent identity: component2486715
display id: component2486715
version: 1
top level: BBa_K2078001
access: public
definition: https://synbiohub.org/public/igem/BBa_J23119/1

BBa_J23119
type: SequenceAnnotation
persistent identity: annotation2486715
display id: annotation2486715
version: 1
top level: BBa_K2078001
location: https://synbiohub.org/public/igem/BBa_K2078001/annotation2486715/range2486715/1
component: BBa_J23119

https://synbiohub.org/public/igem/BBa_K2078001/annotation2486715/range2486715/1
type: Range
persistent identity: range2486715
display id: range2486715
version: 1
direction: direction/reverse
top level: BBa_K2078001
start: 1
end: 35
orientation: inline

BBa_K2078000
type: SequenceAnnotation
persistent identity: annotation2486719
display id: annotation2486719
version: 1
top level: BBa_K2078001
location: https://synbiohub.org/public/igem/BBa_K2078001/annotation2486719/range2486719/1
component: BBa_K2078000

https://synbiohub.org/public/igem/BBa_K2078001/annotation2486719/range2486719/1
type: Range
persistent identity: range2486719
display id: range2486719
version: 1
direction: direction/reverse
top level: BBa_K2078001
start: 44
end: 1418
orientation: inline

BBa_K141000
type: ComponentDefinition
persistent identity: BBa K141000
display id: BBa_K141000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ucp1
creator: Jaume For??s Martos
created: 2008-10-05T11:00:00Z
modified: 2015-05-08T01:10:18Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _229_
m user id: 0
owner id: 2947
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The Ucp1 is a DNA part which encodes for a protein called UCP1 which belongs to the uncuopling proteins family. The uncoupling proteins (UCPs) are transporters, presents in the mitochondrial inner membrane, that mediate a regulated discharge of the proton gradient that is generated by the respiratory chain. This energy-dissipatory mechanism can serve functions such as termogenesis. The protein is regulated by acivation and inhibition in the mitochondrion. The proton conductance in UCP1 is inhibited by nucleotides and activated by fatty acids.
mutable notes: none
mutable provenance: Uncouplin protein UCP1 is a protein that is found in the mitochondria from brown adipocytes.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K141000_sequence/1