//biosafety/xnase
type: Collection
persistent identity: biosafety xnase
display id: biosafety_xnase
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K526001
member: BBa_K914002
member: BBa_K1172904
member: pLac ColE2
member: ColE2
member: BBa_K1479016
member: test
member: BBa_K150009

BBa_K914002
type: ComponentDefinition
persistent identity: BBa K914002
display id: BBa_K914002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: repressilator RBS Colicin E2 Immunity protein
creator: Julianne Rieders
created: 2012-09-18T11:00:00Z
modified: 2015-05-08T01:13:45Z
discontinued: false
dominant: false
group u list: _1179_
m user id: 0
owner id: 13463
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Colicin E2 Immunity protein with the repressilator RBS
mutable notes: We used the full repressilator RBS
mutable provenance: pDEW Colicin E2
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
sequence annotation: BBa_K914002
sequence annotation: cei
sequence annotation: repressilator
sequence: https://synbiohub.org/public/igem/BBa_K914002_sequence/1

BBa_K914002
type: SequenceAnnotation
persistent identity: annotation2187416
display id: annotation2187416
version: 1
top level: BBa_K914002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K914002/annotation2187416/range2187416/1

https://synbiohub.org/public/igem/BBa_K914002/annotation2187416/range2187416/1
type: Range
persistent identity: range2187416
display id: range2187416
version: 1
direction: direction/reverse
top level: BBa_K914002
start: 1
end: 282
orientation: inline

cei
type: SequenceAnnotation
persistent identity: annotation2187417
display id: annotation2187417
version: 1
top level: BBa_K914002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K914002/annotation2187417/range2187417/1

https://synbiohub.org/public/igem/BBa_K914002/annotation2187417/range2187417/1
type: Range
persistent identity: range2187417
display id: range2187417
version: 1
direction: direction/forward
top level: BBa_K914002
start: 22
end: 282
orientation: inline

repressilator
type: SequenceAnnotation
persistent identity: annotation2187415
display id: annotation2187415
version: 1
top level: BBa_K914002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K914002/annotation2187415/range2187415/1

https://synbiohub.org/public/igem/BBa_K914002/annotation2187415/range2187415/1
type: Range
persistent identity: range2187415
display id: range2187415
version: 1
direction: direction/forward
top level: BBa_K914002
start: 1
end: 21
orientation: inline

BBa_K1479016
type: ComponentDefinition
persistent identity: BBa K1479016
display id: BBa_K1479016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1859_
m user id: 0
owner id: 21516
owning group id: 9
sample status: No part sequence
status: status/Planning
bookmark: false
mutable description: fsadfasdfasdfasfdasdfa
mutable notes: sadfasdfadfsdfa
mutable provenance: adsfsdfasdfasdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Promoter
sequence annotation: 1
sequence annotation: 4

1
type: SequenceAnnotation
persistent identity: annotation2418316
display id: annotation2418316
version: 1
top level: BBa_K1479016
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1479016/annotation2418316/range2418316/1

https://synbiohub.org/public/igem/BBa_K1479016/annotation2418316/range2418316/1
type: Range
persistent identity: range2418316
display id: range2418316
version: 1
direction: direction/forward
top level: BBa_K1479016
start: 2
end: 3
orientation: inline

4
type: SequenceAnnotation
persistent identity: annotation2418317
display id: annotation2418317
version: 1
top level: BBa_K1479016
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1479016/annotation2418317/range2418317/1

https://synbiohub.org/public/igem/BBa_K1479016/annotation2418317/range2418317/1
type: Range
persistent identity: range2418317
display id: range2418317
version: 1
direction: direction/forward
top level: BBa_K1479016
start: 5
end: 6
orientation: inline

BBa_K150009
type: ComponentDefinition
persistent identity: BBa K150009
display id: BBa_K150009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ColicinE1 Producer Controlled by 3OC6HSL Receiver Device
creator: Pascal Kraemer, Marika Ziesack, Kathrin Nussbaum and Andreas Kuehne
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:10:46Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _234_
m user id: 0
owner id: 3299
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The constitutive expressed LuxR protein is able to build a complex with the AHL molecule. This complex function as transcription factor for the lux pR promoter and activates by this the colicinE1 production. This activation of the lux pR promoter can additionally lead to expression of the kil protein by read through of the terminator t1 and therefore to lysis of the cell and colcin release. The colicinE1 immunity protein (imm) lying on the reverse strand is expressend under a constitutive promotor.
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_R0062
component: BBa_K150107
component: BBa_K150008
component: BBa_C0062
component: BBa_K150100
component: BBa_K150007
component: BBa_K150005
component: BBa_K150104
component: BBa_R0040
component: BBa_B0034
component: BBa_B0010
component: BBa_B0012
sequence annotation: BBa_K150100
sequence annotation: BBa_R0062
sequence annotation: BBa_B0012
sequence annotation: BBa_K150107
sequence annotation: BBa_B0010
sequence annotation: BBa_K150007
sequence annotation: BBa_K150005
sequence annotation: BBa_K150104
sequence annotation: BBa_C0062
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_K150008
sequence: https://synbiohub.org/public/igem/BBa_K150009_sequence/1

BBa_R0062
type: Component
persistent identity: component2305749
display id: component2305749
version: 1
top level: BBa_K150009
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_K150107
type: Component
persistent identity: component2305732
display id: component2305732
version: 1
top level: BBa_K150009
access: public
definition: https://synbiohub.org/public/igem/BBa_K150107/1

BBa_K150008
type: Component
persistent identity: component2305757
display id: component2305757
version: 1
top level: BBa_K150009
access: public
definition: https://synbiohub.org/public/igem/BBa_K150008/1

BBa_C0062
type: Component
persistent identity: component2305735
display id: component2305735
version: 1
top level: BBa_K150009
access: public
definition: https://synbiohub.org/public/igem/BBa_C0062/1

BBa_K150100
type: Component
persistent identity: component2305742
display id: component2305742
version: 1
top level: BBa_K150009
access: public
definition: https://synbiohub.org/public/igem/BBa_K150100/1

BBa_K150007
type: Component
persistent identity: component2305761
display id: component2305761
version: 1
top level: BBa_K150009
access: public
definition: https://synbiohub.org/public/igem/BBa_K150007/1

BBa_K150005
type: Component
persistent identity: component2305756
display id: component2305756
version: 1
top level: BBa_K150009
access: public
definition: https://synbiohub.org/public/igem/BBa_K150005/1

BBa_K150100
type: Component
persistent identity: component2305739
display id: component2305739
version: 1
top level: BBa_K150009
access: public
definition: https://synbiohub.org/public/igem/BBa_K150100/1

BBa_K150100
type: Component
persistent identity: component2305747
display id: component2305747
version: 1
top level: BBa_K150009
access: public
definition: https://synbiohub.org/public/igem/BBa_K150100/1

BBa_K150100
type: Component
persistent identity: component2305729
display id: component2305729
version: 1
top level: BBa_K150009
access: public
definition: https://synbiohub.org/public/igem/BBa_K150100/1

BBa_K150104
type: Component
persistent identity: component2305754
display id: component2305754
version: 1
top level: BBa_K150009
access: public
definition: https://synbiohub.org/public/igem/BBa_K150104/1

BBa_R0040
type: Component
persistent identity: component2305724
display id: component2305724
version: 1
top level: BBa_K150009
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0034
type: Component
persistent identity: component2305731
display id: component2305731
version: 1
top level: BBa_K150009
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component2305740
display id: component2305740
version: 1
top level: BBa_K150009
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component2305743
display id: component2305743
version: 1
top level: BBa_K150009
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_K150100
type: SequenceAnnotation
persistent identity: annotation2305742
display id: annotation2305742
version: 1
top level: BBa_K150009
location: https://synbiohub.org/public/igem/BBa_K150009/annotation2305742/range2305742/1
component: BBa_K150100

https://synbiohub.org/public/igem/BBa_K150009/annotation2305742/range2305742/1
type: Range
persistent identity: range2305742
display id: range2305742
version: 1
direction: direction/reverse
top level: BBa_K150009
start: 950
end: 957
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2305749
display id: annotation2305749
version: 1
top level: BBa_K150009
location: https://synbiohub.org/public/igem/BBa_K150009/annotation2305749/range2305749/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K150009/annotation2305749/range2305749/1
type: Range
persistent identity: range2305749
display id: range2305749
version: 1
direction: direction/forward
top level: BBa_K150009
start: 1007
end: 1061
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2305743
display id: annotation2305743
version: 1
top level: BBa_K150009
location: https://synbiohub.org/public/igem/BBa_K150009/annotation2305743/range2305743/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K150009/annotation2305743/range2305743/1
type: Range
persistent identity: range2305743
display id: range2305743
version: 1
direction: direction/forward
top level: BBa_K150009
start: 958
end: 998
orientation: inline

BBa_K150107
type: SequenceAnnotation
persistent identity: annotation2305732
display id: annotation2305732
version: 1
top level: BBa_K150009
location: https://synbiohub.org/public/igem/BBa_K150009/annotation2305732/range2305732/1
component: BBa_K150107

https://synbiohub.org/public/igem/BBa_K150009/annotation2305732/range2305732/1
type: Range
persistent identity: range2305732
display id: range2305732
version: 1
direction: direction/reverse
top level: BBa_K150009
start: 75
end: 80
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2305740
display id: annotation2305740
version: 1
top level: BBa_K150009
location: https://synbiohub.org/public/igem/BBa_K150009/annotation2305740/range2305740/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K150009/annotation2305740/range2305740/1
type: Range
persistent identity: range2305740
display id: range2305740
version: 1
direction: direction/forward
top level: BBa_K150009
start: 870
end: 949
orientation: inline

BBa_K150100
type: SequenceAnnotation
persistent identity: annotation2305739
display id: annotation2305739
version: 1
top level: BBa_K150009
location: https://synbiohub.org/public/igem/BBa_K150009/annotation2305739/range2305739/1
component: BBa_K150100

https://synbiohub.org/public/igem/BBa_K150009/annotation2305739/range2305739/1
type: Range
persistent identity: range2305739
display id: range2305739
version: 1
direction: direction/reverse
top level: BBa_K150009
start: 862
end: 869
orientation: inline

BBa_K150100
type: SequenceAnnotation
persistent identity: annotation2305729
display id: annotation2305729
version: 1
top level: BBa_K150009
location: https://synbiohub.org/public/igem/BBa_K150009/annotation2305729/range2305729/1
component: BBa_K150100

https://synbiohub.org/public/igem/BBa_K150009/annotation2305729/range2305729/1
type: Range
persistent identity: range2305729
display id: range2305729
version: 1
direction: direction/reverse
top level: BBa_K150009
start: 55
end: 62
orientation: inline

BBa_K150007
type: SequenceAnnotation
persistent identity: annotation2305761
display id: annotation2305761
version: 1
top level: BBa_K150009
location: https://synbiohub.org/public/igem/BBa_K150009/annotation2305761/range2305761/1
component: BBa_K150007

https://synbiohub.org/public/igem/BBa_K150009/annotation2305761/range2305761/1
type: Range
persistent identity: range2305761
display id: range2305761
version: 1
direction: direction/reverse
top level: BBa_K150009
start: 1089
end: 3180
orientation: inline

BBa_K150005
type: SequenceAnnotation
persistent identity: annotation2305756
display id: annotation2305756
version: 1
top level: BBa_K150009
location: https://synbiohub.org/public/igem/BBa_K150009/annotation2305756/range2305756/1
component: BBa_K150005

https://synbiohub.org/public/igem/BBa_K150009/annotation2305756/range2305756/1
type: Range
persistent identity: range2305756
display id: range2305756
version: 1
direction: direction/reverse
top level: BBa_K150009
start: 1070
end: 1082
orientation: inline

BBa_K150104
type: SequenceAnnotation
persistent identity: annotation2305754
display id: annotation2305754
version: 1
top level: BBa_K150009
location: https://synbiohub.org/public/igem/BBa_K150009/annotation2305754/range2305754/1
component: BBa_K150104

https://synbiohub.org/public/igem/BBa_K150009/annotation2305754/range2305754/1
type: Range
persistent identity: range2305754
display id: range2305754
version: 1
direction: direction/reverse
top level: BBa_K150009
start: 1062
end: 1069
orientation: inline

BBa_K150100
type: SequenceAnnotation
persistent identity: annotation2305747
display id: annotation2305747
version: 1
top level: BBa_K150009
location: https://synbiohub.org/public/igem/BBa_K150009/annotation2305747/range2305747/1
component: BBa_K150100

https://synbiohub.org/public/igem/BBa_K150009/annotation2305747/range2305747/1
type: Range
persistent identity: range2305747
display id: range2305747
version: 1
direction: direction/reverse
top level: BBa_K150009
start: 999
end: 1006
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation2305735
display id: annotation2305735
version: 1
top level: BBa_K150009
location: https://synbiohub.org/public/igem/BBa_K150009/annotation2305735/range2305735/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_K150009/annotation2305735/range2305735/1
type: Range
persistent identity: range2305735
display id: range2305735
version: 1
direction: direction/forward
top level: BBa_K150009
start: 81
end: 836
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2305724
display id: annotation2305724
version: 1
top level: BBa_K150009
location: https://synbiohub.org/public/igem/BBa_K150009/annotation2305724/range2305724/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K150009/annotation2305724/range2305724/1
type: Range
persistent identity: range2305724
display id: range2305724
version: 1
direction: direction/forward
top level: BBa_K150009
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2305731
display id: annotation2305731
version: 1
top level: BBa_K150009
location: https://synbiohub.org/public/igem/BBa_K150009/annotation2305731/range2305731/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K150009/annotation2305731/range2305731/1
type: Range
persistent identity: range2305731
display id: range2305731
version: 1
direction: direction/reverse
top level: BBa_K150009
start: 63
end: 74
orientation: inline

BBa_K150008
type: SequenceAnnotation
persistent identity: annotation2305757
display id: annotation2305757
version: 1
top level: BBa_K150009
location: https://synbiohub.org/public/igem/BBa_K150009/annotation2305757/range2305757/1
component: BBa_K150008

https://synbiohub.org/public/igem/BBa_K150009/annotation2305757/range2305757/1
type: Range
persistent identity: range2305757
display id: range2305757
version: 1
direction: direction/reverse
top level: BBa_K150009
start: 1083
end: 1088
orientation: inline

test
type: ComponentDefinition
persistent identity: BBa K1479017
display id: BBa_K1479017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: just for test
creator: tester
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1859_
m user id: 0
owner id: 21516
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: dsfadfadsfa
mutable notes: fafafdfafa
mutable provenance: fadsfasdfaf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: sdfafds
sequence annotation: dfdsf
sequence: https://synbiohub.org/public/igem/BBa_K1479017_sequence/1

sdfafds
type: SequenceAnnotation
persistent identity: annotation2400465
display id: annotation2400465
version: 1
top level: test
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1479017/annotation2400465/range2400465/1

https://synbiohub.org/public/igem/BBa_K1479017/annotation2400465/range2400465/1
type: Range
persistent identity: range2400465
display id: range2400465
version: 1
direction: direction/forward
top level: test
start: 1
end: 13
orientation: inline

dfdsf
type: SequenceAnnotation
persistent identity: annotation2400466
display id: annotation2400466
version: 1
top level: test
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1479017/annotation2400466/range2400466/1

https://synbiohub.org/public/igem/BBa_K1479017/annotation2400466/range2400466/1
type: Range
persistent identity: range2400466
display id: range2400466
version: 1
direction: direction/forward
top level: test
start: 2
end: 21
orientation: inline

pLac ColE2
type: ComponentDefinition
persistent identity: BBa K914001
display id: BBa_K914001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLac Colicin E2 Immunity protein
creator: Julianne Rieders
created: 2012-09-18T11:00:00Z
modified: 2015-05-08T01:13:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1179_
m user id: 0
owner id: 13463
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Colicin E2 Immunity protein under pLac promoter (BBa_R0011) with repressilator RBS. This part can be used to confer immunity against the colicin E2 activity protein by induction with IPTG.
mutable notes: We selected the repressilator RBS and pLac to drive production of Colicin E2 Immunity protein
mutable provenance: pDEW Colicin E2
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: lac O1
sequence annotation: -10
sequence annotation: repressilator
sequence annotation: -35
sequence annotation: cei
sequence annotation: BBa_R0011
sequence annotation: BBa_K914002
sequence: https://synbiohub.org/public/igem/BBa_K914001_sequence/1

lac O1
type: SequenceAnnotation
persistent identity: annotation2188656
display id: annotation2188656
version: 1
top level: pLac ColE2
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K914001/annotation2188656/range2188656/1

https://synbiohub.org/public/igem/BBa_K914001/annotation2188656/range2188656/1
type: Range
persistent identity: range2188656
display id: range2188656
version: 1
direction: direction/forward
top level: pLac ColE2
start: 26
end: 42
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2188657
display id: annotation2188657
version: 1
top level: pLac ColE2
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K914001/annotation2188657/range2188657/1

https://synbiohub.org/public/igem/BBa_K914001/annotation2188657/range2188657/1
type: Range
persistent identity: range2188657
display id: range2188657
version: 1
direction: direction/forward
top level: pLac ColE2
start: 43
end: 48
orientation: inline

repressilator
type: SequenceAnnotation
persistent identity: annotation2188658
display id: annotation2188658
version: 1
top level: pLac ColE2
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K914001/annotation2188658/range2188658/1

https://synbiohub.org/public/igem/BBa_K914001/annotation2188658/range2188658/1
type: Range
persistent identity: range2188658
display id: range2188658
version: 1
direction: direction/forward
top level: pLac ColE2
start: 64
end: 84
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2188655
display id: annotation2188655
version: 1
top level: pLac ColE2
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K914001/annotation2188655/range2188655/1

https://synbiohub.org/public/igem/BBa_K914001/annotation2188655/range2188655/1
type: Range
persistent identity: range2188655
display id: range2188655
version: 1
direction: direction/forward
top level: pLac ColE2
start: 20
end: 25
orientation: inline

cei
type: SequenceAnnotation
persistent identity: annotation2188660
display id: annotation2188660
version: 1
top level: pLac ColE2
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K914001/annotation2188660/range2188660/1

https://synbiohub.org/public/igem/BBa_K914001/annotation2188660/range2188660/1
type: Range
persistent identity: range2188660
display id: range2188660
version: 1
direction: direction/forward
top level: pLac ColE2
start: 85
end: 345
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2188653
display id: annotation2188653
version: 1
top level: pLac ColE2
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K914001/annotation2188653/range2188653/1

https://synbiohub.org/public/igem/BBa_K914001/annotation2188653/range2188653/1
type: Range
persistent identity: range2188653
display id: range2188653
version: 1
direction: direction/forward
top level: pLac ColE2
start: 1
end: 54
orientation: inline

BBa_K914002
type: SequenceAnnotation
persistent identity: annotation2188659
display id: annotation2188659
version: 1
top level: pLac ColE2
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K914001/annotation2188659/range2188659/1

https://synbiohub.org/public/igem/BBa_K914001/annotation2188659/range2188659/1
type: Range
persistent identity: range2188659
display id: range2188659
version: 1
direction: direction/reverse
top level: pLac ColE2
start: 64
end: 345
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation2188654
display id: annotation2188654
version: 1
top level: pLac ColE2
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K914001/annotation2188654/range2188654/1

https://synbiohub.org/public/igem/BBa_K914001/annotation2188654/range2188654/1
type: Range
persistent identity: range2188654
display id: range2188654
version: 1
direction: direction/forward
top level: pLac ColE2
start: 3
end: 19
orientation: inline

BBa_K526001
type: ComponentDefinition
persistent identity: BBa K526001
display id: BBa_K526001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ptrc*-Dpn
creator: Sung Kuk Lee
created: 2011-09-25T11:00:00Z
modified: 2015-05-08T01:12:35Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _692_
m user id: 0
owner id: 8432
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: ptrc* is a modified form of ptrc promoter with the -35 and -10 regions being present in the reverse order and the promoter is flanked by fimE recognition sequence. FimE is an invertase capable of inverting any DNA sequence flanked between its recognition seqence. This fim inversion system would help avoid leaky expression. We expressed a new lysis device, DpnI under the control of fim inversion promoter. DpnI is a restriction enzyme capable of chopping up all methylated DNAs. We propose DpnI as a novel lysis device. DpnC and DpnD are obtained from Streptococcus pneumoniae R6 strain.
mutable notes: One should be careful while expressing Dpn genes. Dpn is supposed to chop up the DNA and cause cell death. Hence, no leaky level of Dpn is needed to recover live cells
mutable provenance: http://www.ncbi.nlm.nih.gov/nuccore/25307955?from=1637214&to=1637675 http://www.ncbi.nlm.nih.gov/nuccore/25307955?from=1637672&to=1638436
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: fim inversion promoter
sequence annotation: DpnD
sequence annotation: DpnC
sequence: https://synbiohub.org/public/igem/BBa_K526001_sequence/1

fim inversion promoter
type: SequenceAnnotation
persistent identity: annotation2150194
display id: annotation2150194
version: 1
top level: BBa_K526001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K526001/annotation2150194/range2150194/1

https://synbiohub.org/public/igem/BBa_K526001/annotation2150194/range2150194/1
type: Range
persistent identity: range2150194
display id: range2150194
version: 1
direction: direction/forward
top level: BBa_K526001
start: 1
end: 220
orientation: inline

DpnD
type: SequenceAnnotation
persistent identity: annotation2150195
display id: annotation2150195
version: 1
top level: BBa_K526001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K526001/annotation2150195/range2150195/1

https://synbiohub.org/public/igem/BBa_K526001/annotation2150195/range2150195/1
type: Range
persistent identity: range2150195
display id: range2150195
version: 1
direction: direction/forward
top level: BBa_K526001
start: 1077
end: 1536
orientation: inline

DpnC
type: SequenceAnnotation
persistent identity: annotation2150193
display id: annotation2150193
version: 1
top level: BBa_K526001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K526001/annotation2150193/range2150193/1

https://synbiohub.org/public/igem/BBa_K526001/annotation2150193/range2150193/1
type: Range
persistent identity: range2150193
display id: range2150193
version: 1
direction: direction/forward
top level: BBa_K526001
start: 221
end: 1046
orientation: inline

BBa_K1172904
type: ComponentDefinition
persistent identity: BBa K1172904
display id: BBa_K1172904
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rnase Ba (Barnase) from Bacillus amyloliquefaciens
creator: Tore Bleckwehl
created: 2013-09-18T11:00:00Z
modified: 2015-05-08T01:09:35Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1485_
m user id: 0
owner id: 16986
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Rnase Ba (Barnase) from Bacillus Amyloliquefaciens
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: K1172904 (RNase Ba)
sequence: https://synbiohub.org/public/igem/BBa_K1172904_sequence/1

K1172904 (RNase Ba)
type: SequenceAnnotation
persistent identity: annotation2357200
display id: annotation2357200
version: 1
top level: BBa_K1172904
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1172904/annotation2357200/range2357200/1

https://synbiohub.org/public/igem/BBa_K1172904/annotation2357200/range2357200/1
type: Range
persistent identity: range2357200
display id: range2357200
version: 1
direction: direction/forward
top level: BBa_K1172904
start: 1
end: 339
orientation: inline

ColE2
type: ComponentDefinition
persistent identity: BBa K131000
display id: BBa_K131000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ColE2 Operon
creator: Kevin McLeod
created: 2008-07-21T11:00:00Z
modified: 2015-05-08T01:09:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _259_
m user id: 0
owner id: 1917
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part contains the ColE2 operon in its entirety.
mutable notes: None?
mutable provenance: This part was cloned from a plasmid containing the operon which was received from UC Berkeley.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: BBa_K131005
sequence annotation: BBa_K131004
sequence annotation: BBa_K131001
sequence annotation: CeiB
sequence annotation: CeaB
sequence annotation: SOS Box
sequence annotation: CelB
sequence annotation: BBa_K131003
sequence annotation: BBa_K131008
sequence annotation: BBa_K131007
sequence annotation: Region of Dyad Symmetry
sequence annotation: -35 Signal
sequence annotation: -15 Signal
sequence: https://synbiohub.org/public/igem/BBa_K131000_sequence/1

BBa_K131005
type: SequenceAnnotation
persistent identity: annotation1968214
display id: annotation1968214
version: 1
top level: ColE2
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K131000/annotation1968214/range1968214/1

https://synbiohub.org/public/igem/BBa_K131000/annotation1968214/range1968214/1
type: Range
persistent identity: range1968214
display id: range1968214
version: 1
direction: direction/forward
top level: ColE2
start: 2401
end: 2544
orientation: inline

BBa_K131004
type: SequenceAnnotation
persistent identity: annotation1968211
display id: annotation1968211
version: 1
top level: ColE2
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K131000/annotation1968211/range1968211/1

https://synbiohub.org/public/igem/BBa_K131000/annotation1968211/range1968211/1
type: Range
persistent identity: range1968211
display id: range1968211
version: 1
direction: direction/forward
top level: ColE2
start: 331
end: 2076
orientation: inline

BBa_K131001
type: SequenceAnnotation
persistent identity: annotation1968210
display id: annotation1968210
version: 1
top level: ColE2
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K131000/annotation1968210/range1968210/1

https://synbiohub.org/public/igem/BBa_K131000/annotation1968210/range1968210/1
type: Range
persistent identity: range1968210
display id: range1968210
version: 1
direction: direction/forward
top level: ColE2
start: 1
end: 267
orientation: inline

CeiB
type: SequenceAnnotation
persistent identity: annotation1968954
display id: annotation1968954
version: 1
top level: ColE2
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K131000/annotation1968954/range1968954/1

https://synbiohub.org/public/igem/BBa_K131000/annotation1968954/range1968954/1
type: Range
persistent identity: range1968954
display id: range1968954
version: 1
direction: direction/forward
top level: ColE2
start: 2079
end: 2081
orientation: inline

CeiB
type: SequenceAnnotation
persistent identity: annotation1968955
display id: annotation1968955
version: 1
top level: ColE2
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K131000/annotation1968955/range1968955/1

https://synbiohub.org/public/igem/BBa_K131000/annotation1968955/range1968955/1
type: Range
persistent identity: range1968955
display id: range1968955
version: 1
direction: direction/forward
top level: ColE2
start: 2337
end: 2339
orientation: inline

CeaB
type: SequenceAnnotation
persistent identity: annotation1968953
display id: annotation1968953
version: 1
top level: ColE2
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K131000/annotation1968953/range1968953/1

https://synbiohub.org/public/igem/BBa_K131000/annotation1968953/range1968953/1
type: Range
persistent identity: range1968953
display id: range1968953
version: 1
direction: direction/forward
top level: ColE2
start: 2074
end: 2076
orientation: inline

SOS Box
type: SequenceAnnotation
persistent identity: annotation1968209
display id: annotation1968209
version: 1
top level: ColE2
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K131000/annotation1968209/range1968209/1

https://synbiohub.org/public/igem/BBa_K131000/annotation1968209/range1968209/1
type: Range
persistent identity: range1968209
display id: range1968209
version: 1
direction: direction/forward
top level: ColE2
start: 252
end: 267
orientation: inline

CelB
type: SequenceAnnotation
persistent identity: annotation1968956
display id: annotation1968956
version: 1
top level: ColE2
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K131000/annotation1968956/range1968956/1

https://synbiohub.org/public/igem/BBa_K131000/annotation1968956/range1968956/1
type: Range
persistent identity: range1968956
display id: range1968956
version: 1
direction: direction/forward
top level: ColE2
start: 2401
end: 2403
orientation: inline

BBa_K131003
type: SequenceAnnotation
persistent identity: annotation1968212
display id: annotation1968212
version: 1
top level: ColE2
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K131000/annotation1968212/range1968212/1

https://synbiohub.org/public/igem/BBa_K131000/annotation1968212/range1968212/1
type: Range
persistent identity: range1968212
display id: range1968212
version: 1
direction: direction/forward
top level: ColE2
start: 2079
end: 2339
orientation: inline

BBa_K131008
type: SequenceAnnotation
persistent identity: annotation1968215
display id: annotation1968215
version: 1
top level: ColE2
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K131000/annotation1968215/range1968215/1

https://synbiohub.org/public/igem/BBa_K131000/annotation1968215/range1968215/1
type: Range
persistent identity: range1968215
display id: range1968215
version: 1
direction: direction/forward
top level: ColE2
start: 2565
end: 2603
orientation: inline

CeaB
type: SequenceAnnotation
persistent identity: annotation1968952
display id: annotation1968952
version: 1
top level: ColE2
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K131000/annotation1968952/range1968952/1

https://synbiohub.org/public/igem/BBa_K131000/annotation1968952/range1968952/1
type: Range
persistent identity: range1968952
display id: range1968952
version: 1
direction: direction/forward
top level: ColE2
start: 331
end: 333
orientation: inline

BBa_K131007
type: SequenceAnnotation
persistent identity: annotation1968213
display id: annotation1968213
version: 1
top level: ColE2
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K131000/annotation1968213/range1968213/1

https://synbiohub.org/public/igem/BBa_K131000/annotation1968213/range1968213/1
type: Range
persistent identity: range1968213
display id: range1968213
version: 1
direction: direction/forward
top level: ColE2
start: 2373
end: 2400
orientation: inline

CelB
type: SequenceAnnotation
persistent identity: annotation1968957
display id: annotation1968957
version: 1
top level: ColE2
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K131000/annotation1968957/range1968957/1

https://synbiohub.org/public/igem/BBa_K131000/annotation1968957/range1968957/1
type: Range
persistent identity: range1968957
display id: range1968957
version: 1
direction: direction/forward
top level: ColE2
start: 2542
end: 2544
orientation: inline

Region of Dyad Symmetry
type: SequenceAnnotation
persistent identity: annotation1968206
display id: annotation1968206
version: 1
top level: ColE2
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K131000/annotation1968206/range1968206/1

https://synbiohub.org/public/igem/BBa_K131000/annotation1968206/range1968206/1
type: Range
persistent identity: range1968206
display id: range1968206
version: 1
direction: direction/forward
top level: ColE2
start: 136
end: 161
orientation: inline

-35 Signal
type: SequenceAnnotation
persistent identity: annotation1968207
display id: annotation1968207
version: 1
top level: ColE2
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K131000/annotation1968207/range1968207/1

https://synbiohub.org/public/igem/BBa_K131000/annotation1968207/range1968207/1
type: Range
persistent identity: range1968207
display id: range1968207
version: 1
direction: direction/forward
top level: ColE2
start: 218
end: 224
orientation: inline

-15 Signal
type: SequenceAnnotation
persistent identity: annotation1968208
display id: annotation1968208
version: 1
top level: ColE2
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K131000/annotation1968208/range1968208/1

https://synbiohub.org/public/igem/BBa_K131000/annotation1968208/range1968208/1
type: Range
persistent identity: range1968208
display id: range1968208
version: 1
direction: direction/forward
top level: ColE2
start: 241
end: 246
orientation: inline