//binding
type: Collection
persistent identity: binding
display id: binding
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: //binding/metal
member: //binding/cellulose

//binding/metal
type: Collection
persistent identity: binding metal
display id: binding_metal
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: BBa_K1420004
member: CP47His
member: BBa_K1509000
member: BBa_K1122703
member: BBa_K1460001
member: BBa_K1460006
member: BBa_K1471000
member: PbrR
member: BBa_K519011
member: BBa_K1505001
member: BBa_K2127002
member: BBa_K1420002
member: BBa_K1980011
member: BBa_K1471001
member: BBa_K1550004
member: BBa_K1701000
member: BBa_K1438003
member: Fur
member: BBa_K2127001
member: BBa_K1460003
member: BBa_K1980010
member: BBa_K1505000
member: MymTsfGFP
member: BBa_K1526007
member: BBa_K190019
member: BFRM52H
member: BBa_I721002
member: BBa_K1438002
member: BBa_K1122702
member: BBa_K643002
member: BBa_K1460004
member: BBa_K190021
member: Csp1
member: BBa_K1460005
member: BBa_K1404002
member: BFR
member: BBa_K346001
member: BBa_K1438035
member: BBa_K1505002
member: BBa_K1980012
member: BBa_K1420003
member: BBa_K1420005
member: BBa_K1393000
member: BBa_K346003
member: MBP
member: BBa_K1460002
member: BBa_K1393001
member: BBa_K519010
member: BBa_K643000
member: BBa_K346004
member: BBa_K1460007
member: MymT
member: BBa_K1420001
member: BBa_K1355001
member: BBa_K160001
member: BBa_K205004
member: BBa_K1342003
member: Csp1sfGFP
member: BBa_K190020
member: BBa_K1694006
member: BBa_K1460008
member: BBa_K1355000

//binding/cellulose
type: Collection
persistent identity: binding cellulose
display id: binding_cellulose
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: BBa_K1321121
member: BBa_K1321340
member: BBa_K1478001
member: BBa_K1321366
member: CBDcex
member: BBa_K1321105
member: BBa_K1321112
member: BBa_K1499004
member: BBa_K1933102
member: BBa_K1321090
member: BBa_K1321093
member: BBa_K1321114
member: BBa_K1321154
member: BBa_K1321352
member: BBa_K1321343
member: BBa_K1321003
member: BBa_K1321115
member: BBa_K1321356
member: BBa_K1321110
member: BBa_K1321351
member: BBa_K1979001
member: BBa_K1321339
member: CBDclos(T7
member: BBa_K1321002
member: BBa_K1321092
member: BBa_K1321122
member: BBa_K1321357
member: BBa_K1321152
member: BBa_K863113
member: BBa_K1321151
member: BBa_K1321014
member: BBa_K1933103
member: BBa_K1321104
member: BBa_K1321091
member: BBa_K1321345
member: BBa_K1321094
member: BBa_K1321353
member: CBDclos(J6
member: BBa_K1321120
member: BBa_K1321346
member: BBa_K1321344
member: BBa_K1933101
member: CBDcex(T7)
member: BBa_K1321111
member: BBa_K1321097
member: BBa_K1933100
member: CBDclos
member: BBa_K1321342
member: BBa_K1321348
member: BBa_K1321096
member: BBa_K1321101
member: CBDcex(J61
member: BBa_K863110
member: BBa_K1321100
member: BBa_K1321095
member: BBa_K1321341
member: BBa_K1321349

BBa_K519010
type: ComponentDefinition
persistent identity: BBa K519010
display id: BBa_K519010
version: 1
was generated by: iGEM to SBOL conversion
description: SmtA
created: 2011-09-29T11:00:00Z
modified: 2016-01-13T10:24:13Z
mutable provenance: Synechococcus sp. PCC7942
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: SmtA is a metallothionein that can bind to heavy metal ions such as Cd(II). This SmtA is cloned from Synechococcus sp. PCC7942 and it has been reported that the cyanobacterial strains that express SmtA can resist medium containing cadmim.
discontinued: false
dominant: true
experience: experience/None
group u list: _684_
m user id: 4206
owner id: 9603
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This part was cloned from Synechococcus sp. PCC7942, using primers to add EcoRI, XbaI and SpeI at the ends.
star: false
creator: Kotone Miyake
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K519010_sequence/1

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 V01146 .
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stop
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

BFRM52H
type: ComponentDefinition
persistent identity: BBa K1438001
display id: BBa_K1438001
version: 1
was generated by: iGEM to SBOL conversion
description: Bacterioferritin (BFR) M52H heme-deletion
created: 2014-10-05T11:00:00Z
modified: 2015-07-22T02:13:13Z
mutable provenance: Escherichia coli Nissle 1917
owned by: james
owned by: myers
mutable description: ATGAAAGGTGATACTAAAGTTATAAATTATCTCAACAAACTGTTGGGAAATGAGCTTGTCGCAATCAATCAGTACTTTCTTCATGCCCGGATGTTTAAAAACTGGGGTCTCAAACGTCTCAATGATGTGGAGTATCATGAATCCATTGATGAGCACAAACACGCCGATCGTTATATTGAGCGCATTCTTTTTCTGGAAGGTCTTCCAAACTTACAGGACCTGGGCAAACTGAACATTGGTGAAGATGTTGAGGAAATGCTGCGTTCTGATCTGGCACTTGAGCTGGATGGCGCGAAGAATTTGCGTGAGGCAATTGGTTATGCCGATAGCGTTCATGATTACGTCAGCCGCGATATGATGATAGAAATTTTGCGCGATGAAGAAGGCCATATCGACTGGCTGGAAACGGAACTTGATCTGATTCAGAAGATGGGACTGCAAAATTATCTGCAAGCACAGATTCGCGAAGAAGGTTAA
discontinued: false
dominant: false
experience: experience/Works
group u list: _1816_
m user id: 4206
owner id: 19714
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: The M52H Mutagensis leads to an deletion of the BFR heme residue. As a result, BFR will have a reduced iron release rate and keep it's iron content inside of the protein scaffold.
star: false
creator: Johann Bauerfeind
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1438001_sequence/1

Scar 25
type: ComponentDefinition
persistent identity: BBa B0105
display id: BBa_B0105
version: 1
was generated by: iGEM to SBOL conversion
description: RFC 25 Scar Sequence
created: 2009-10-14T11:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: BBF RFC 25
owned by: james
owned by: myers
mutable description: This is the scar produced by assembly using RFC 25. If you are assembling a composite part using RFC 25, you can insert this part and specify blunt assembly to get the desired sequence.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 25
owning group id: 397
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Simple DNA sequence
star: false
creator: Randy Rettberg
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Scar 25
sequence: https://synbiohub.org/public/igem/BBa_B0105_sequence/1

Scar 25
type: SequenceAnnotation
persistent identity: annotation2041621
display id: annotation2041621
version: 1
top level: Scar 25
role: feature/dna
role: SO:0000110
location: https://synbiohub.org/public/igem/BBa_B0105/annotation2041621/range2041621/1

https://synbiohub.org/public/igem/BBa_B0105/annotation2041621/range2041621/1
type: Range
persistent identity: range2041621
display id: range2041621
version: 1
top level: Scar 25
direction: direction/forward
start: 1
end: 6
orientation: inline

BBa_K1321111
type: ComponentDefinition
persistent identity: BBa K1321111
display id: BBa_K1321111
version: 1
was generated by: iGEM to SBOL conversion
description: Phytochelatin (PC) EC20 fused with CBDclos with LacI promoter
created: 2014-10-08T11:00:00Z
modified: 2015-06-17T12:19:36Z
mutable provenance: PhytoC+CBD4 with LacI
owned by: james
owned by: myers
mutable description: PhytoC+CBD4 with LacI
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20780
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: PhytoC+CBD4 with LacI
star: false
creator: Xenia Spencer-Milnes
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1319106
component: BBa_K1321002
component: BBa_K1321005
component: BBa_B0105
component: BBa_J04500
sequence annotation: BBa_K1321002
sequence annotation: BBa_B0105
sequence annotation: BBa_J04500
sequence annotation: BBa_K1321005
sequence annotation: BBa_K1319106
sequence: https://synbiohub.org/public/igem/BBa_K1321111_sequence/1

BBa_K1319106
type: Component
persistent identity: component2414887
display id: component2414887
version: 1
top level: BBa_K1321111
access: public
definition: Scar25 ATG

BBa_K1321002
type: Component
persistent identity: component2414892
display id: component2414892
version: 1
top level: BBa_K1321111
access: public
definition: BBa_K1321002

BBa_K1321005
type: Component
persistent identity: component2414889
display id: component2414889
version: 1
top level: BBa_K1321111
access: public
definition: BBa_K1321005

BBa_B0105
type: Component
persistent identity: component2414891
display id: component2414891
version: 1
top level: BBa_K1321111
access: public
definition: Scar 25

BBa_J04500
type: Component
persistent identity: component2414885
display id: component2414885
version: 1
top level: BBa_K1321111
access: public
definition: BBa_J04500

BBa_K1321002
type: SequenceAnnotation
persistent identity: annotation2414892
display id: annotation2414892
version: 1
top level: BBa_K1321111
location: https://synbiohub.org/public/igem/BBa_K1321111/annotation2414892/range2414892/1
component: BBa_K1321002

https://synbiohub.org/public/igem/BBa_K1321111/annotation2414892/range2414892/1
type: Range
persistent identity: range2414892
display id: range2414892
version: 1
top level: BBa_K1321111
direction: direction/reverse
start: 365
end: 664
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2414891
display id: annotation2414891
version: 1
top level: BBa_K1321111
location: https://synbiohub.org/public/igem/BBa_K1321111/annotation2414891/range2414891/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321111/annotation2414891/range2414891/1
type: Range
persistent identity: range2414891
display id: range2414891
version: 1
top level: BBa_K1321111
direction: direction/reverse
start: 359
end: 364
orientation: inline

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2414885
display id: annotation2414885
version: 1
top level: BBa_K1321111
location: https://synbiohub.org/public/igem/BBa_K1321111/annotation2414885/range2414885/1
component: BBa_J04500

https://synbiohub.org/public/igem/BBa_K1321111/annotation2414885/range2414885/1
type: Range
persistent identity: range2414885
display id: range2414885
version: 1
top level: BBa_K1321111
direction: direction/reverse
start: 1
end: 220
orientation: inline

BBa_K1321005
type: SequenceAnnotation
persistent identity: annotation2414889
display id: annotation2414889
version: 1
top level: BBa_K1321111
location: https://synbiohub.org/public/igem/BBa_K1321111/annotation2414889/range2414889/1
component: BBa_K1321005

https://synbiohub.org/public/igem/BBa_K1321111/annotation2414889/range2414889/1
type: Range
persistent identity: range2414889
display id: range2414889
version: 1
top level: BBa_K1321111
direction: direction/reverse
start: 236
end: 358
orientation: inline

BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2414887
display id: annotation2414887
version: 1
top level: BBa_K1321111
location: https://synbiohub.org/public/igem/BBa_K1321111/annotation2414887/range2414887/1
component: BBa_K1319106

https://synbiohub.org/public/igem/BBa_K1321111/annotation2414887/range2414887/1
type: Range
persistent identity: range2414887
display id: range2414887
version: 1
top level: BBa_K1321111
direction: direction/reverse
start: 221
end: 235
orientation: inline

BBa_K1321353
type: ComponentDefinition
persistent identity: BBa K1321353
display id: BBa_K1321353
version: 1
was generated by: iGEM to SBOL conversion
description: NiBP fused to CBDclos driven by T7
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:32:03Z
mutable provenance: asdf
owned by: james
owned by: myers
mutable description: asdf
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: asdf
star: false
creator: Chris N Micklem
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1319106
component: BBa_K1321338
component: BBa_K1321343
sequence annotation: BBa_K1319106
sequence annotation: BBa_K1321343
sequence annotation: BBa_K1321338
sequence: https://synbiohub.org/public/igem/BBa_K1321353_sequence/1

BBa_K1319106
type: Component
persistent identity: component2415405
display id: component2415405
version: 1
top level: BBa_K1321353
access: public
definition: Scar25 ATG

BBa_K1321338
type: Component
persistent identity: component2415403
display id: component2415403
version: 1
top level: BBa_K1321353
access: public
definition: BBa_K1321338

BBa_K1321343
type: Component
persistent identity: component2415410
display id: component2415410
version: 1
top level: BBa_K1321353
access: public
definition: BBa_K1321343

BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2415405
display id: annotation2415405
version: 1
top level: BBa_K1321353
location: https://synbiohub.org/public/igem/BBa_K1321353/annotation2415405/range2415405/1
component: BBa_K1319106

https://synbiohub.org/public/igem/BBa_K1321353/annotation2415405/range2415405/1
type: Range
persistent identity: range2415405
display id: range2415405
version: 1
top level: BBa_K1321353
direction: direction/reverse
start: 44
end: 58
orientation: inline

BBa_K1321343
type: SequenceAnnotation
persistent identity: annotation2415410
display id: annotation2415410
version: 1
top level: BBa_K1321353
location: https://synbiohub.org/public/igem/BBa_K1321353/annotation2415410/range2415410/1
component: BBa_K1321343

https://synbiohub.org/public/igem/BBa_K1321353/annotation2415410/range2415410/1
type: Range
persistent identity: range2415410
display id: range2415410
version: 1
top level: BBa_K1321353
direction: direction/reverse
start: 59
end: 540
orientation: inline

BBa_K1321338
type: SequenceAnnotation
persistent identity: annotation2415403
display id: annotation2415403
version: 1
top level: BBa_K1321353
location: https://synbiohub.org/public/igem/BBa_K1321353/annotation2415403/range2415403/1
component: BBa_K1321338

https://synbiohub.org/public/igem/BBa_K1321353/annotation2415403/range2415403/1
type: Range
persistent identity: range2415403
display id: range2415403
version: 1
top level: BBa_K1321353
direction: direction/reverse
start: 1
end: 43
orientation: inline

BBa_K1933100
type: ComponentDefinition
persistent identity: BBa K1933100
display id: BBa_K1933100
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive expression of CBDcex fused to INPNC with 6xHis tag
created: 2016-10-09T11:00:00Z
modified: 2016-10-17T11:35:27Z
mutable provenance: Promoter and RBS: The INPNC surface expression component derives from Pseudomonas Syringae. We used parts BBa_K811005 from iGEM12_Penn. CBDcex derives from Cellulomonas fimi, and is part of an exoglucanase secreted by this organism. We used parts BBa_K1321342 from iGEM Imperial 2014 for construction.
owned by: james
owned by: myers
mutable description: CBDcex fused to INPNC with 6xHis tag is one of a series of surface expressing fusion proteins that make up biodevice that aims to be therapeutic solution against norovirus infections. This protein in particular is a cellulose binding protein(CBDcex) fused to surface expression anchoring domain(INPNC), connected by a poly-histidine tag to be easily identified by Westernblotting. For more information, please visit our Wiki page.
discontinued: false
dominant: false
experience: experience/Works
group u list: _2400_
m user id: 23701
owner id: 29998
owning group id: 9
status: status/Planning
bookmark: true
mutable notes: The stop codon for this part is not in the coding region, but in the suffix region.
star: false
creator: Tomoki Uchino
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1321003
component: BBa_K1933004
component: BBa_K1933011
sequence annotation: BBa_K1321003
sequence annotation: BBa_K1933004
sequence annotation: BBa_K1933011
sequence: https://synbiohub.org/public/igem/BBa_K1933100_sequence/1

BBa_K1321003
type: Component
persistent identity: component2516443
display id: component2516443
version: 1
top level: BBa_K1933100
access: public
definition: BBa_K1321003

BBa_K1933004
type: Component
persistent identity: component2516438
display id: component2516438
version: 1
top level: BBa_K1933100
access: public
definition: BBa_K1933004

BBa_K1933011
type: Component
persistent identity: component2516442
display id: component2516442
version: 1
top level: BBa_K1933100
access: public
definition: BBa_K1933011

BBa_K1321003
type: SequenceAnnotation
persistent identity: annotation2516443
display id: annotation2516443
version: 1
top level: BBa_K1933100
location: https://synbiohub.org/public/igem/BBa_K1933100/annotation2516443/range2516443/1
component: BBa_K1321003

https://synbiohub.org/public/igem/BBa_K1933100/annotation2516443/range2516443/1
type: Range
persistent identity: range2516443
display id: range2516443
version: 1
top level: BBa_K1933100
direction: direction/reverse
start: 1036
end: 1362
orientation: inline

BBa_K1933004
type: SequenceAnnotation
persistent identity: annotation2516438
display id: annotation2516438
version: 1
top level: BBa_K1933100
location: https://synbiohub.org/public/igem/BBa_K1933100/annotation2516438/range2516438/1
component: BBa_K1933004

https://synbiohub.org/public/igem/BBa_K1933100/annotation2516438/range2516438/1
type: Range
persistent identity: range2516438
display id: range2516438
version: 1
top level: BBa_K1933100
direction: direction/reverse
start: 1
end: 60
orientation: inline

BBa_K1933011
type: SequenceAnnotation
persistent identity: annotation2516442
display id: annotation2516442
version: 1
top level: BBa_K1933100
location: https://synbiohub.org/public/igem/BBa_K1933100/annotation2516442/range2516442/1
component: BBa_K1933011

https://synbiohub.org/public/igem/BBa_K1933100/annotation2516442/range2516442/1
type: Range
persistent identity: range2516442
display id: range2516442
version: 1
top level: BBa_K1933100
direction: direction/reverse
start: 61
end: 1035
orientation: inline

BBa_K1460002
type: ComponentDefinition
persistent identity: BBa K1460002
display id: BBa_K1460002
version: 1
was generated by: iGEM to SBOL conversion
description: RBS + GST (glutathione-S-transferase)-CRS5 (metallothionein) + Ter
created: 2014-08-13T11:00:00Z
modified: 2016-01-13T10:26:04Z
mutable provenance: This part is BBa_K1460001 with the T7 promoter removed.
owned by: james
owned by: myers
mutable description: his part serves as a functional metallothionein for heavy metal binding and conferred resistance to heavy metals when combined with an appropriate promoter. CRS5 gene is the metallothionein that binds to heavy metals and it is fused to GST for stability.
discontinued: false
dominant: false
experience: experience/None
group u list: _1839_
m user id: 4206
owner id: 15649
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The part contains a KpnI cut site between the biobrick prefix and the RBS that is a result of removing the T7 promoter from part BBa_K1460001.
star: false
creator: Eric Holmes
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_B1006
sequence annotation: CRS5
sequence annotation: Linker Sequence
sequence annotation: BBa_J61101
sequence annotation: GST
sequence: https://synbiohub.org/public/igem/BBa_K1460002_sequence/1

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2380991
display id: annotation2380991
version: 1
top level: BBa_K1460002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1460002/annotation2380991/range2380991/1

https://synbiohub.org/public/igem/BBa_K1460002/annotation2380991/range2380991/1
type: Range
persistent identity: range2380991
display id: range2380991
version: 1
top level: BBa_K1460002
direction: direction/forward
start: 931
end: 969
orientation: inline

CRS5
type: SequenceAnnotation
persistent identity: annotation2380990
display id: annotation2380990
version: 1
top level: BBa_K1460002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1460002/annotation2380990/range2380990/1

https://synbiohub.org/public/igem/BBa_K1460002/annotation2380990/range2380990/1
type: Range
persistent identity: range2380990
display id: range2380990
version: 1
top level: BBa_K1460002
direction: direction/forward
start: 721
end: 930
orientation: inline

Linker Sequence
type: SequenceAnnotation
persistent identity: annotation2380989
display id: annotation2380989
version: 1
top level: BBa_K1460002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1460002/annotation2380989/range2380989/1

https://synbiohub.org/public/igem/BBa_K1460002/annotation2380989/range2380989/1
type: Range
persistent identity: range2380989
display id: range2380989
version: 1
top level: BBa_K1460002
direction: direction/forward
start: 688
end: 720
orientation: inline

BBa_J61101
type: SequenceAnnotation
persistent identity: annotation2380987
display id: annotation2380987
version: 1
top level: BBa_K1460002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1460002/annotation2380987/range2380987/1

https://synbiohub.org/public/igem/BBa_K1460002/annotation2380987/range2380987/1
type: Range
persistent identity: range2380987
display id: range2380987
version: 1
top level: BBa_K1460002
direction: direction/forward
start: 14
end: 27
orientation: inline

GST
type: SequenceAnnotation
persistent identity: annotation2380988
display id: annotation2380988
version: 1
top level: BBa_K1460002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1460002/annotation2380988/range2380988/1

https://synbiohub.org/public/igem/BBa_K1460002/annotation2380988/range2380988/1
type: Range
persistent identity: range2380988
display id: range2380988
version: 1
top level: BBa_K1460002
direction: direction/forward
start: 34
end: 687
orientation: inline

BBa_K1321091
type: ComponentDefinition
persistent identity: BBa K1321091
display id: BBa_K1321091
version: 1
was generated by: iGEM to SBOL conversion
description: Phytochelatin (PC) EC20 fused to CBDclos
created: 2014-10-06T11:00:00Z
modified: 2015-06-17T12:34:24Z
mutable provenance: PhytoC+CBD4. TBC
owned by: james
owned by: myers
mutable description: PhytoC+CBD4. TBC
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20780
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: PhytoC+CBD4. TBC
star: false
creator: Xenia Spencer-Milnes
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321005
component: BBa_B0105
component: BBa_K1321002
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321002
sequence annotation: BBa_K1321005
sequence: https://synbiohub.org/public/igem/BBa_K1321091_sequence/1

BBa_K1321005
type: Component
persistent identity: component2414578
display id: component2414578
version: 1
top level: BBa_K1321091
access: public
definition: BBa_K1321005

BBa_B0105
type: Component
persistent identity: component2414580
display id: component2414580
version: 1
top level: BBa_K1321091
access: public
definition: Scar 25

BBa_K1321002
type: Component
persistent identity: component2414581
display id: component2414581
version: 1
top level: BBa_K1321091
access: public
definition: BBa_K1321002

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2414580
display id: annotation2414580
version: 1
top level: BBa_K1321091
location: https://synbiohub.org/public/igem/BBa_K1321091/annotation2414580/range2414580/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321091/annotation2414580/range2414580/1
type: Range
persistent identity: range2414580
display id: range2414580
version: 1
top level: BBa_K1321091
direction: direction/reverse
start: 142
end: 147
orientation: inline

BBa_K1321002
type: SequenceAnnotation
persistent identity: annotation2414581
display id: annotation2414581
version: 1
top level: BBa_K1321091
location: https://synbiohub.org/public/igem/BBa_K1321091/annotation2414581/range2414581/1
component: BBa_K1321002

https://synbiohub.org/public/igem/BBa_K1321091/annotation2414581/range2414581/1
type: Range
persistent identity: range2414581
display id: range2414581
version: 1
top level: BBa_K1321091
direction: direction/reverse
start: 148
end: 447
orientation: inline

BBa_K1321005
type: SequenceAnnotation
persistent identity: annotation2414578
display id: annotation2414578
version: 1
top level: BBa_K1321091
location: https://synbiohub.org/public/igem/BBa_K1321091/annotation2414578/range2414578/1
component: BBa_K1321005

https://synbiohub.org/public/igem/BBa_K1321091/annotation2414578/range2414578/1
type: Range
persistent identity: range2414578
display id: range2414578
version: 1
top level: BBa_K1321091
direction: direction/reverse
start: 1
end: 141
orientation: inline

BBa_K1321366
type: ComponentDefinition
persistent identity: BBa K1321366
display id: BBa_K1321366
version: 1
was generated by: iGEM to SBOL conversion
description: CBDcex fused to sfGFP with T7 promoter
created: 2014-10-08T11:00:00Z
modified: 2016-01-26T09:46:05Z
mutable provenance: GATC
owned by: james
owned by: myers
mutable description: GATC
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20961
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: GATC
star: false
creator: Gabriella Santosa
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1319106
component: BBa_K1321349
component: BBa_K1321338
sequence annotation: BBa_K1319106
sequence annotation: BBa_K1321349
sequence annotation: BBa_K1321338
sequence: https://synbiohub.org/public/igem/BBa_K1321366_sequence/1

BBa_K1319106
type: Component
persistent identity: component2415726
display id: component2415726
version: 1
top level: BBa_K1321366
access: public
definition: Scar25 ATG

BBa_K1321349
type: Component
persistent identity: component2415732
display id: component2415732
version: 1
top level: BBa_K1321366
access: public
definition: BBa_K1321349

BBa_K1321338
type: Component
persistent identity: component2415724
display id: component2415724
version: 1
top level: BBa_K1321366
access: public
definition: BBa_K1321338

BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2415726
display id: annotation2415726
version: 1
top level: BBa_K1321366
location: https://synbiohub.org/public/igem/BBa_K1321366/annotation2415726/range2415726/1
component: BBa_K1319106

https://synbiohub.org/public/igem/BBa_K1321366/annotation2415726/range2415726/1
type: Range
persistent identity: range2415726
display id: range2415726
version: 1
top level: BBa_K1321366
direction: direction/reverse
start: 44
end: 58
orientation: inline

BBa_K1321349
type: SequenceAnnotation
persistent identity: annotation2415732
display id: annotation2415732
version: 1
top level: BBa_K1321366
location: https://synbiohub.org/public/igem/BBa_K1321366/annotation2415732/range2415732/1
component: BBa_K1321349

https://synbiohub.org/public/igem/BBa_K1321366/annotation2415732/range2415732/1
type: Range
persistent identity: range2415732
display id: range2415732
version: 1
top level: BBa_K1321366
direction: direction/reverse
start: 59
end: 1102
orientation: inline

BBa_K1321338
type: SequenceAnnotation
persistent identity: annotation2415724
display id: annotation2415724
version: 1
top level: BBa_K1321366
location: https://synbiohub.org/public/igem/BBa_K1321366/annotation2415724/range2415724/1
component: BBa_K1321338

https://synbiohub.org/public/igem/BBa_K1321366/annotation2415724/range2415724/1
type: Range
persistent identity: range2415724
display id: range2415724
version: 1
top level: BBa_K1321366
direction: direction/reverse
start: 1
end: 43
orientation: inline

BBa_K1509000
type: ComponentDefinition
persistent identity: BBa K1509000
display id: BBa_K1509000
version: 1
was generated by: iGEM to SBOL conversion
description: Coding for the trans-acting regulator SmtB
created: 2014-09-25T11:00:00Z
modified: 2015-07-23T03:26:04Z
mutable provenance: Metallothioneins(MTs), discovered in horse kidney in 1957 by Margoshes and Vallee are identified as low-molecular weight and sulphydryl rich proteins which bind metal ions in metal-thiolate clusters and whose synthesis increases in response to elevated concentrations of certain metals. In 1988, Olafson and co-workers reported the amino acid sequence of a metal-associated protein isolated from cyanobacterium Synechococcus sp.. With the function of binding metal ions remaining, it only has less than 20% homology in amino acid sequence with known MTs in eukaryotes and thus we call it class II MT or on some occasions MT-like. Owing to the works of Robinson and Huckle, the corresponding locus of Synechococcus PCC7942, smt locus was isolated and analyzed. It includes smtA which encodes the class II MT and a divergently transcribed gene smtB which encodes a trans-acting, autoregulatory repressor that exerts metal ion-inducible negative control over smtA transcription. Between them lies a 100-bp operator-promoter region(smt O-P, BBa_K1509001), which is responsible for the divergent orientation of smtA and smtB.
owned by: james
owned by: myers
mutable description: In 1993, Huckle and co-workers reported the isolation and analysis of the smt locus from Synechococcus sp.. Gene smtB, one of the components of the locus, encodes a transcriptional repressor of gene smtA, with which is required for normal tolerance to Zn2+ and Cd2+. The product of smtB functions by binding to the smt operator-promoter(smtO-P) and dissociating in the presence of Zn2+ due to the classical helix-turn-helix motif similar to many DNA-binding proteins. It can also be genomically rearranged when induced by Cd2+, leading to a deletion within smtB because of a highly iterated palindrome (HIP1), a highly represented octanucleotides(5′-GCGATC-GC-3′) which traverse both borders of the excised element. These two mechanisms both cause the elevated expression of smtA.
discontinued: false
dominant: false
group u list: _1889_
m user id: 4206
owner id: 24032
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: According to the mechanisms of smt locus represented above, gene smtB should function with the accompany of smt operator-promoter. Previous cases selected smtA whose product functions by encoding MT-like to sequester metal ions, while our case intended to take advantage of the trigger lies in this locus. By imitating former researches, in which a promoter less lacZ gene was fused upstream of smtA, we respectively splice two selected reporter genes and sequence from smt operator-promoter to smtB, equivalent to replacing smtA with reporter genes, to visualize the effects of metal ions on the locus.
star: false
creator: Tong Li
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: smtB
sequence: https://synbiohub.org/public/igem/BBa_K1509000_sequence/1

smtB
type: SequenceAnnotation
persistent identity: annotation2387081
display id: annotation2387081
version: 1
top level: BBa_K1509000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1509000/annotation2387081/range2387081/1

https://synbiohub.org/public/igem/BBa_K1509000/annotation2387081/range2387081/1
type: Range
persistent identity: range2387081
display id: range2387081
version: 1
top level: BBa_K1509000
direction: direction/forward
start: 1
end: 301
orientation: inline

BBa_K1321092
type: ComponentDefinition
persistent identity: BBa K1321092
display id: BBa_K1321092
version: 1
was generated by: iGEM to SBOL conversion
description: Phytochelatin (PC) EC20 fused to CBDcex
created: 2014-10-06T11:00:00Z
modified: 2015-06-17T12:17:32Z
mutable provenance: PhytoC+CBD7. TBC
owned by: james
owned by: myers
mutable description: PhytoC+CBD7. TBC
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20780
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: PhytoC+CBD7. TBC
star: false
creator: Xenia Spencer-Milnes
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_B0105
component: BBa_K1321003
component: BBa_K1321005
sequence annotation: BBa_K1321003
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321005
sequence: https://synbiohub.org/public/igem/BBa_K1321092_sequence/1

BBa_B0105
type: Component
persistent identity: component2414620
display id: component2414620
version: 1
top level: BBa_K1321092
access: public
definition: Scar 25

BBa_K1321003
type: Component
persistent identity: component2414621
display id: component2414621
version: 1
top level: BBa_K1321092
access: public
definition: BBa_K1321003

BBa_K1321005
type: Component
persistent identity: component2414618
display id: component2414618
version: 1
top level: BBa_K1321092
access: public
definition: BBa_K1321005

BBa_K1321003
type: SequenceAnnotation
persistent identity: annotation2414621
display id: annotation2414621
version: 1
top level: BBa_K1321092
location: https://synbiohub.org/public/igem/BBa_K1321092/annotation2414621/range2414621/1
component: BBa_K1321003

https://synbiohub.org/public/igem/BBa_K1321092/annotation2414621/range2414621/1
type: Range
persistent identity: range2414621
display id: range2414621
version: 1
top level: BBa_K1321092
direction: direction/reverse
start: 130
end: 456
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2414620
display id: annotation2414620
version: 1
top level: BBa_K1321092
location: https://synbiohub.org/public/igem/BBa_K1321092/annotation2414620/range2414620/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321092/annotation2414620/range2414620/1
type: Range
persistent identity: range2414620
display id: range2414620
version: 1
top level: BBa_K1321092
direction: direction/reverse
start: 124
end: 129
orientation: inline

BBa_K1321005
type: SequenceAnnotation
persistent identity: annotation2414618
display id: annotation2414618
version: 1
top level: BBa_K1321092
location: https://synbiohub.org/public/igem/BBa_K1321092/annotation2414618/range2414618/1
component: BBa_K1321005

https://synbiohub.org/public/igem/BBa_K1321092/annotation2414618/range2414618/1
type: Range
persistent identity: range2414618
display id: range2414618
version: 1
top level: BBa_K1321092
direction: direction/reverse
start: 1
end: 123
orientation: inline

BBa_K1460003
type: ComponentDefinition
persistent identity: BBa K1460003
display id: BBa_K1460003
version: 1
was generated by: iGEM to SBOL conversion
description: Anderson Promoter + NixA + Ter
created: 2014-08-13T11:00:00Z
modified: 2016-01-13T10:21:44Z
mutable provenance: Anderson promoter is that of part BBa_J23102. Terminator is that of part BBa_B1006. NixA gene is naturally found in H. pylori. This part was synthesized by GenScript in the vector pUC57. Part was then transferred into pSB1C3 using standard cloning procedures.
owned by: james
owned by: myers
mutable description: This is a functional coding sequence for the protein NixA, which is a nickel transporter from H. pylori. This part will result in nickel intolerance from higher levels of intracellular nickel.
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1839_
m user id: 4206
owner id: 15649
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The Anderson promoter provides strong constitutive expression of NixA in E. coli.
star: false
creator: Eric Holmes
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_B1006
sequence annotation: BBa_J23102
sequence annotation: NixA
sequence annotation: BBa_J61101
sequence: https://synbiohub.org/public/igem/BBa_K1460003_sequence/1

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2381016
display id: annotation2381016
version: 1
top level: BBa_K1460003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1460003/annotation2381016/range2381016/1

https://synbiohub.org/public/igem/BBa_K1460003/annotation2381016/range2381016/1
type: Range
persistent identity: range2381016
display id: range2381016
version: 1
top level: BBa_K1460003
direction: direction/forward
start: 990
end: 1028
orientation: inline

BBa_J23102
type: SequenceAnnotation
persistent identity: annotation2381013
display id: annotation2381013
version: 1
top level: BBa_K1460003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1460003/annotation2381013/range2381013/1

https://synbiohub.org/public/igem/BBa_K1460003/annotation2381013/range2381013/1
type: Range
persistent identity: range2381013
display id: range2381013
version: 1
top level: BBa_K1460003
direction: direction/forward
start: 1
end: 35
orientation: inline

NixA
type: SequenceAnnotation
persistent identity: annotation2381015
display id: annotation2381015
version: 1
top level: BBa_K1460003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1460003/annotation2381015/range2381015/1

https://synbiohub.org/public/igem/BBa_K1460003/annotation2381015/range2381015/1
type: Range
persistent identity: range2381015
display id: range2381015
version: 1
top level: BBa_K1460003
direction: direction/forward
start: 63
end: 989
orientation: inline

BBa_J61101
type: SequenceAnnotation
persistent identity: annotation2381014
display id: annotation2381014
version: 1
top level: BBa_K1460003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1460003/annotation2381014/range2381014/1

https://synbiohub.org/public/igem/BBa_K1460003/annotation2381014/range2381014/1
type: Range
persistent identity: range2381014
display id: range2381014
version: 1
top level: BBa_K1460003
direction: direction/forward
start: 43
end: 56
orientation: inline

BBa_K1420005
type: ComponentDefinition
persistent identity: BBa K1420005
display id: BBa_K1420005
version: 1
was generated by: iGEM to SBOL conversion
description: merT, mercuric transport protein
created: 2014-10-08T11:00:00Z
modified: 2015-07-22T03:01:03Z
mutable provenance: Source organism: Serratia marcescens. Amplified from plasmid pDU1358, kindly donated by Anne O. Summers.
owned by: james
owned by: myers
mutable description: merT is found in the Serratia marcescens plasmid pDU1358 on the other side of the bidirectional promoter opposite of merR. It is 351 base pairs long and is hypothesized that it codes for a mercuric transport protein.
discontinued: false
dominant: false
group u list: _1798_
m user id: 4206
owner id: 17872
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: false
creator: Stephen C. Heinsch
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: merT
sequence: https://synbiohub.org/public/igem/BBa_K1420005_sequence/1

merT
type: SequenceAnnotation
persistent identity: annotation2407640
display id: annotation2407640
version: 1
top level: BBa_K1420005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1420005/annotation2407640/range2407640/1

https://synbiohub.org/public/igem/BBa_K1420005/annotation2407640/range2407640/1
type: Range
persistent identity: range2407640
display id: range2407640
version: 1
top level: BBa_K1420005
direction: direction/forward
start: 1
end: 351
orientation: inline

BclA-His
type: ComponentDefinition
persistent identity: BBa K1933000
display id: BBa_K1933000
version: 1
was generated by: iGEM to SBOL conversion
description: N-terminal domain of BclA protein fused to 6xHis tag
created: 2016-10-09T11:00:00Z
modified: 2016-10-17T12:19:04Z
mutable provenance: NCBI accession number CAD56880 NCBI accession number CAD56878 NCBI accession number CAD56869 NCBI accession number CAD56870
owned by: james
owned by: myers
mutable description: This part codes N-terminal domain of BclA protein which expressed on the cell surface connected by a 6??His tag to be easily identified by Western blotting.[1] Surface displayed passengers retain enzyme activity even when it is linked to BclA with 6?? His tag.
discontinued: false
dominant: false
experience: experience/Works
group u list: _2400_
m user id: 23701
owner id: 29998
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: optimized to expression in E. coli K-12 strain
star: false
creator: Tomoki Uchino
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BclA
sequence annotation: 6xHis tag
sequence annotation: start codon
sequence: https://synbiohub.org/public/igem/BBa_K1933000_sequence/1

BclA
type: SequenceAnnotation
persistent identity: annotation2490249
display id: annotation2490249
version: 1
top level: BclA-His
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1933000/annotation2490249/range2490249/1

https://synbiohub.org/public/igem/BBa_K1933000/annotation2490249/range2490249/1
type: Range
persistent identity: range2490249
display id: range2490249
version: 1
top level: BclA-His
direction: direction/forward
start: 4
end: 66
orientation: inline

6xHis tag
type: SequenceAnnotation
persistent identity: annotation2490250
display id: annotation2490250
version: 1
top level: BclA-His
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1933000/annotation2490250/range2490250/1

https://synbiohub.org/public/igem/BBa_K1933000/annotation2490250/range2490250/1
type: Range
persistent identity: range2490250
display id: range2490250
version: 1
top level: BclA-His
direction: direction/forward
start: 67
end: 84
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2490248
display id: annotation2490248
version: 1
top level: BclA-His
role: feature/start
role: SO:0000318
location: https://synbiohub.org/public/igem/BBa_K1933000/annotation2490248/range2490248/1

https://synbiohub.org/public/igem/BBa_K1933000/annotation2490248/range2490248/1
type: Range
persistent identity: range2490248
display id: range2490248
version: 1
top level: BclA-His
direction: direction/forward
start: 1
end: 3
orientation: inline

BBa_K1393001
type: ComponentDefinition
persistent identity: BBa K1393001
display id: BBa_K1393001
version: 1
was generated by: iGEM to SBOL conversion
description: OprF(Ala196)+CBP
created: 2014-10-01T11:00:00Z
modified: 2015-07-22T02:48:08Z
mutable provenance: The OprF gene is from Pseudomonas aeruginosa.
owned by: james
owned by: myers
mutable description: The OprF is a major outer membrane protein of Pseudomonas aeruginosa. This protein functions as a nonspecific porin to allow the passage of small hydrophilic molecules, plays a structural role in maintaining cell shape and outer membrane integrity, and is required for growth under low osmolality. The structure of OprF has been proposed to consist of three domains, the N-terminal forming h-barrel structure, a loop or hinge region, and the C-terminal associated with peptidoglycan. Based on the predicted secondary structure and information found in the literature, we chose Ala196 as potential fusion sites for displaying CBP. CBP is the abbreviation of copper binding peptide made up of seven amino acid.
discontinued: false
dominant: false
group u list: _1770_
m user id: 4206
owner id: 20256
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Based on the predicted secondary structure and information found in the literature, we chose Val(188) as potential fusion sites for displaying CBP. CBP is the abbreviation of copper binding peptide made up of seven amino acid.
star: false
creator: Jiajun Tan
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: oprF to Ala196
sequence annotation: CBP
sequence: https://synbiohub.org/public/igem/BBa_K1393001_sequence/1

oprF to Ala196
type: SequenceAnnotation
persistent identity: annotation2391711
display id: annotation2391711
version: 1
top level: BBa_K1393001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1393001/annotation2391711/range2391711/1

https://synbiohub.org/public/igem/BBa_K1393001/annotation2391711/range2391711/1
type: Range
persistent identity: range2391711
display id: range2391711
version: 1
top level: BBa_K1393001
direction: direction/forward
start: 1
end: 588
orientation: inline

CBP
type: SequenceAnnotation
persistent identity: annotation2391717
display id: annotation2391717
version: 1
top level: BBa_K1393001
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1393001/annotation2391717/range2391717/1

https://synbiohub.org/public/igem/BBa_K1393001/annotation2391717/range2391717/1
type: Range
persistent identity: range2391717
display id: range2391717
version: 1
top level: BBa_K1393001
direction: direction/forward
start: 589
end: 609
orientation: inline

BBa_K1321357
type: ComponentDefinition
persistent identity: BBa K1321357
display id: BBa_K1321357
version: 1
was generated by: iGEM to SBOL conversion
description: sfGFP fused to CBDcex driven by LacI
created: 2014-10-07T11:00:00Z
modified: 2016-01-26T09:45:49Z
mutable provenance: asdf
owned by: james
owned by: myers
mutable description: asdf
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: asdf
star: false
creator: Chris N Micklem
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1321342
component: BBa_J04500
component: BBa_K1319106
sequence annotation: BBa_K1319106
sequence annotation: BBa_K1321342
sequence annotation: BBa_J04500
sequence: https://synbiohub.org/public/igem/BBa_K1321357_sequence/1

BBa_K1321342
type: Component
persistent identity: component2415507
display id: component2415507
version: 1
top level: BBa_K1321357
access: public
definition: BBa_K1321342

BBa_J04500
type: Component
persistent identity: component2415499
display id: component2415499
version: 1
top level: BBa_K1321357
access: public
definition: BBa_J04500

BBa_K1319106
type: Component
persistent identity: component2415501
display id: component2415501
version: 1
top level: BBa_K1321357
access: public
definition: Scar25 ATG

BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2415501
display id: annotation2415501
version: 1
top level: BBa_K1321357
location: https://synbiohub.org/public/igem/BBa_K1321357/annotation2415501/range2415501/1
component: BBa_K1319106

https://synbiohub.org/public/igem/BBa_K1321357/annotation2415501/range2415501/1
type: Range
persistent identity: range2415501
display id: range2415501
version: 1
top level: BBa_K1321357
direction: direction/reverse
start: 221
end: 235
orientation: inline

BBa_K1321342
type: SequenceAnnotation
persistent identity: annotation2415507
display id: annotation2415507
version: 1
top level: BBa_K1321357
location: https://synbiohub.org/public/igem/BBa_K1321357/annotation2415507/range2415507/1
component: BBa_K1321342

https://synbiohub.org/public/igem/BBa_K1321357/annotation2415507/range2415507/1
type: Range
persistent identity: range2415507
display id: range2415507
version: 1
top level: BBa_K1321357
direction: direction/reverse
start: 236
end: 1279
orientation: inline

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2415499
display id: annotation2415499
version: 1
top level: BBa_K1321357
location: https://synbiohub.org/public/igem/BBa_K1321357/annotation2415499/range2415499/1
component: BBa_J04500

https://synbiohub.org/public/igem/BBa_K1321357/annotation2415499/range2415499/1
type: Range
persistent identity: range2415499
display id: range2415499
version: 1
top level: BBa_K1321357
direction: direction/reverse
start: 1
end: 220
orientation: inline

BBa_K1321093
type: ComponentDefinition
persistent identity: BBa K1321093
display id: BBa_K1321093
version: 1
was generated by: iGEM to SBOL conversion
description: Phytochelatin (PC) EC20 + Linker-CBDcipA-Linker
created: 2014-10-08T11:00:00Z
modified: 2015-06-17T12:35:00Z
mutable provenance: GATC
owned by: james
owned by: myers
mutable description: GATC
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20961
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: GATC
star: false
creator: Gabriella Santosa
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321014
component: BBa_B0105
component: BBa_K1321005
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321014
sequence annotation: BBa_K1321005
sequence: https://synbiohub.org/public/igem/BBa_K1321093_sequence/1

BBa_K1321014
type: Component
persistent identity: component2423517
display id: component2423517
version: 1
top level: BBa_K1321093
access: public
definition: BBa_K1321014

BBa_B0105
type: Component
persistent identity: component2423513
display id: component2423513
version: 1
top level: BBa_K1321093
access: public
definition: Scar 25

BBa_K1321005
type: Component
persistent identity: component2423511
display id: component2423511
version: 1
top level: BBa_K1321093
access: public
definition: BBa_K1321005

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2423513
display id: annotation2423513
version: 1
top level: BBa_K1321093
location: https://synbiohub.org/public/igem/BBa_K1321093/annotation2423513/range2423513/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321093/annotation2423513/range2423513/1
type: Range
persistent identity: range2423513
display id: range2423513
version: 1
top level: BBa_K1321093
direction: direction/reverse
start: 124
end: 129
orientation: inline

BBa_K1321014
type: SequenceAnnotation
persistent identity: annotation2423517
display id: annotation2423517
version: 1
top level: BBa_K1321093
location: https://synbiohub.org/public/igem/BBa_K1321093/annotation2423517/range2423517/1
component: BBa_K1321014

https://synbiohub.org/public/igem/BBa_K1321093/annotation2423517/range2423517/1
type: Range
persistent identity: range2423517
display id: range2423517
version: 1
top level: BBa_K1321093
direction: direction/reverse
start: 130
end: 840
orientation: inline

BBa_K1321005
type: SequenceAnnotation
persistent identity: annotation2423511
display id: annotation2423511
version: 1
top level: BBa_K1321093
location: https://synbiohub.org/public/igem/BBa_K1321093/annotation2423511/range2423511/1
component: BBa_K1321005

https://synbiohub.org/public/igem/BBa_K1321093/annotation2423511/range2423511/1
type: Range
persistent identity: range2423511
display id: range2423511
version: 1
top level: BBa_K1321093
direction: direction/reverse
start: 1
end: 123
orientation: inline

BBa_K1505000
type: ComponentDefinition
persistent identity: BBa K1505000
display id: BBa_K1505000
version: 1
was generated by: iGEM to SBOL conversion
description: RBS+mntH
created: 2014-10-08T11:00:00Z
modified: 2016-01-13T10:32:21Z
mutable provenance: Azospirillum sp. B510 genome
owned by: james
owned by: myers
mutable description: The gene was functionally expressed in Azospirillum sp. B510. It encodes a divalent metal cation transporter. The protein can be used to increase transportation level of divalent metal ions into the cells. Its potential applications include heavy metal bioremediation (by increasing uptake efficiency) and protein expression in cell surface (by fusion with mntH).
discontinued: false
dominant: false
experience: experience/None
group u list: _1885_
m user id: 4206
owner id: 22465
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: It is the coding sequence of MntH
star: false
creator: Renyao Wei
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: rbs (from pmsp3)
sequence annotation: mntH
sequence: https://synbiohub.org/public/igem/BBa_K1505000_sequence/1

rbs (from pmsp3)
type: SequenceAnnotation
persistent identity: annotation2412322
display id: annotation2412322
version: 1
top level: BBa_K1505000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1505000/annotation2412322/range2412322/1

https://synbiohub.org/public/igem/BBa_K1505000/annotation2412322/range2412322/1
type: Range
persistent identity: range2412322
display id: range2412322
version: 1
top level: BBa_K1505000
direction: direction/forward
start: 19
end: 46
orientation: inline

mntH
type: SequenceAnnotation
persistent identity: annotation2412323
display id: annotation2412323
version: 1
top level: BBa_K1505000
role: feature/protein
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1505000/annotation2412323/range2412323/1

https://synbiohub.org/public/igem/BBa_K1505000/annotation2412323/range2412323/1
type: Range
persistent identity: range2412323
display id: range2412323
version: 1
top level: BBa_K1505000
direction: direction/forward
start: 67
end: 1302
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_K1471001
type: ComponentDefinition
persistent identity: BBa K1471001
display id: BBa_K1471001
version: 1
was generated by: iGEM to SBOL conversion
description: MerB.
created: 2014-10-07T11:00:00Z
modified: 2015-07-22T02:40:08Z
mutable provenance: Genomic sequence
owned by: james
owned by: myers
mutable description: The MerB gene codes for the proetin which function is to cleave the organic part of the molecule of methyl-mercury
discontinued: false
dominant: false
experience: experience/None
group u list: _1850_
m user id: 4206
owner id: 16614
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: efwgeg
star: false
creator: Jaime Antonio del Castillo Nu??ez
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: MerB
sequence: https://synbiohub.org/public/igem/BBa_K1471001_sequence/1

MerB
type: SequenceAnnotation
persistent identity: annotation2419568
display id: annotation2419568
version: 1
top level: BBa_K1471001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1471001/annotation2419568/range2419568/1

https://synbiohub.org/public/igem/BBa_K1471001/annotation2419568/range2419568/1
type: Range
persistent identity: range2419568
display id: range2419568
version: 1
top level: BBa_K1471001
direction: direction/forward
start: 1
end: 639
orientation: inline

BBa_K1321349
type: ComponentDefinition
persistent identity: BBa K1321349
display id: BBa_K1321349
version: 1
was generated by: iGEM to SBOL conversion
description: CBDcex fused to sfGFP in RFC 25
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:30:55Z
mutable provenance: asdf
owned by: james
owned by: myers
mutable description: asdf
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: asdf
star: false
creator: Chris N Micklem
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321337
component: BBa_B0105
component: BBa_K1321003
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321337
sequence annotation: BBa_K1321003
sequence: https://synbiohub.org/public/igem/BBa_K1321349_sequence/1

BBa_K1321337
type: Component
persistent identity: component2415153
display id: component2415153
version: 1
top level: BBa_K1321349
access: public
definition: BBa_K1321337

BBa_B0105
type: Component
persistent identity: component2415151
display id: component2415151
version: 1
top level: BBa_K1321349
access: public
definition: Scar 25

BBa_K1321003
type: Component
persistent identity: component2415149
display id: component2415149
version: 1
top level: BBa_K1321349
access: public
definition: BBa_K1321003

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2415151
display id: annotation2415151
version: 1
top level: BBa_K1321349
location: https://synbiohub.org/public/igem/BBa_K1321349/annotation2415151/range2415151/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321349/annotation2415151/range2415151/1
type: Range
persistent identity: range2415151
display id: range2415151
version: 1
top level: BBa_K1321349
direction: direction/reverse
start: 328
end: 333
orientation: inline

BBa_K1321337
type: SequenceAnnotation
persistent identity: annotation2415153
display id: annotation2415153
version: 1
top level: BBa_K1321349
location: https://synbiohub.org/public/igem/BBa_K1321349/annotation2415153/range2415153/1
component: BBa_K1321337

https://synbiohub.org/public/igem/BBa_K1321349/annotation2415153/range2415153/1
type: Range
persistent identity: range2415153
display id: range2415153
version: 1
top level: BBa_K1321349
direction: direction/reverse
start: 334
end: 1044
orientation: inline

BBa_K1321003
type: SequenceAnnotation
persistent identity: annotation2415149
display id: annotation2415149
version: 1
top level: BBa_K1321349
location: https://synbiohub.org/public/igem/BBa_K1321349/annotation2415149/range2415149/1
component: BBa_K1321003

https://synbiohub.org/public/igem/BBa_K1321349/annotation2415149/range2415149/1
type: Range
persistent identity: range2415149
display id: range2415149
version: 1
top level: BBa_K1321349
direction: direction/reverse
start: 1
end: 327
orientation: inline

Fur
type: ComponentDefinition
persistent identity: BBa K1122666
display id: BBa_K1122666
version: 1
was generated by: iGEM to SBOL conversion
description: Ferric uptake repressor
created: 2013-07-23T11:00:00Z
modified: 2015-07-24T01:14:24Z
mutable provenance: This comes from Bacillus subtilis.
owned by: myers
owned by: james
mutable description: The ferric uptake repressor of Bacillus subtilis called YqkL is a regulator that when in contact with iron will bind and repress a regulatory sequence (the Fur box).
discontinued: false
dominant: false
experience: experience/None
group u list: _1433_
m user id: 4206
owner id: 18022
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: We sequenced the Fur box. This can be added in front of genes to provide regulation when in contact with iron.
star: false
creator: Hugo Villanueva
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1122666_sequence/1

BBa_K1321152
type: ComponentDefinition
persistent identity: BBa K1321152
display id: BBa_K1321152
version: 1
was generated by: iGEM to SBOL conversion
description: metallothionein Fmt fused to CBDcex
created: 2014-10-08T11:00:00Z
modified: 2015-06-17T12:23:40Z
mutable provenance: GATC
owned by: james
owned by: myers
mutable description: GATC
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20961
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: GATC
star: false
creator: Gabriella Santosa
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321003
component: BBa_B0105
component: BBa_K1321012
sequence annotation: BBa_K1321012
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321003
sequence: https://synbiohub.org/public/igem/BBa_K1321152_sequence/1

BBa_K1321003
type: Component
persistent identity: component2415040
display id: component2415040
version: 1
top level: BBa_K1321152
access: public
definition: BBa_K1321003

BBa_B0105
type: Component
persistent identity: component2415039
display id: component2415039
version: 1
top level: BBa_K1321152
access: public
definition: Scar 25

BBa_K1321012
type: Component
persistent identity: component2415037
display id: component2415037
version: 1
top level: BBa_K1321152
access: public
definition: BBa_K1321012

BBa_K1321012
type: SequenceAnnotation
persistent identity: annotation2415037
display id: annotation2415037
version: 1
top level: BBa_K1321152
location: https://synbiohub.org/public/igem/BBa_K1321152/annotation2415037/range2415037/1
component: BBa_K1321012

https://synbiohub.org/public/igem/BBa_K1321152/annotation2415037/range2415037/1
type: Range
persistent identity: range2415037
display id: range2415037
version: 1
top level: BBa_K1321152
direction: direction/reverse
start: 1
end: 198
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2415039
display id: annotation2415039
version: 1
top level: BBa_K1321152
location: https://synbiohub.org/public/igem/BBa_K1321152/annotation2415039/range2415039/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321152/annotation2415039/range2415039/1
type: Range
persistent identity: range2415039
display id: range2415039
version: 1
top level: BBa_K1321152
direction: direction/reverse
start: 199
end: 204
orientation: inline

BBa_K1321003
type: SequenceAnnotation
persistent identity: annotation2415040
display id: annotation2415040
version: 1
top level: BBa_K1321152
location: https://synbiohub.org/public/igem/BBa_K1321152/annotation2415040/range2415040/1
component: BBa_K1321003

https://synbiohub.org/public/igem/BBa_K1321152/annotation2415040/range2415040/1
type: Range
persistent identity: range2415040
display id: range2415040
version: 1
top level: BBa_K1321152
direction: direction/reverse
start: 205
end: 531
orientation: inline

BBa_K1393000
type: ComponentDefinition
persistent identity: BBa K1393000
display id: BBa_K1393000
version: 1
was generated by: iGEM to SBOL conversion
description: OprF(Val188)+GS linker+CBP
created: 2014-10-01T11:00:00Z
modified: 2015-07-22T02:47:15Z
mutable provenance: The OprF gene is from Pseudomonas aeruginosa.
owned by: james
owned by: myers
mutable description: The OprF is a major outer membrane protein of Pseudomonas aeruginosa. This protein functions as a nonspecific porin to allow the passage of small hydrophilic molecules, plays a structural role in maintaining cell shape and outer membrane integrity, and is required for growth under low osmolality.The structure of OprF has been proposed to consist of three domains, the N-terminal forming h-barrel structure, a loop or hinge region, and the C-terminal associated with peptidoglycan.
discontinued: false
dominant: false
group u list: _1770_
m user id: 4206
owner id: 20256
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Based on the predicted secondary structure and information found in the literature, we chose Val(188）as potential fusion sites for displaying CBP.CBP is the abbreviation of copper binding peptide made up of seven amino acid.In order to reduce the effect on the CBP activity，we added (G4S) linker between OprF and CBP .
star: false
creator: Jiajun Tan
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: CBP
sequence annotation: OprF to Val188
sequence: https://synbiohub.org/public/igem/BBa_K1393000_sequence/1

CBP
type: SequenceAnnotation
persistent identity: annotation2392395
display id: annotation2392395
version: 1
top level: BBa_K1393000
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1393000/annotation2392395/range2392395/1

https://synbiohub.org/public/igem/BBa_K1393000/annotation2392395/range2392395/1
type: Range
persistent identity: range2392395
display id: range2392395
version: 1
top level: BBa_K1393000
direction: direction/forward
start: 579
end: 600
orientation: inline

OprF to Val188
type: SequenceAnnotation
persistent identity: annotation2392394
display id: annotation2392394
version: 1
top level: BBa_K1393000
role: feature/protein
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1393000/annotation2392394/range2392394/1

https://synbiohub.org/public/igem/BBa_K1393000/annotation2392394/range2392394/1
type: Range
persistent identity: range2392394
display id: range2392394
version: 1
top level: BBa_K1393000
direction: direction/forward
start: 1
end: 564
orientation: inline

BBa_K346001
type: ComponentDefinition
persistent identity: BBa K346001
display id: BBa_K346001
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (B0034) + MerR (mercury-responsive transcription factor)
created: 2010-10-07T11:00:00Z
modified: 2015-07-21T02:53:47Z
mutable provenance: The coding sequence of MerR comes from Tn, prefixed by an RBS part BBa_B0034 from Partsregistry
owned by: james
owned by: myers
mutable description: This part was designed as a translational unit for MerR expression.
discontinued: false
dominant: false
experience: experience/Works
group u list: _353_457_
m user id: 4206
owner id: 4411
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: The native RBS of MerR is not strong enough and its intensity can not be predicted as there is no such a corresponding RBS part in Registry. In order to facilitate the use and further characterization of MerR for future team, we prefixed an RBS part BBa_B0034 from Partsregistry.
star: false
creator: Ao Liu & Ying Sheng
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: merR
sequence annotation: B0034
sequence: https://synbiohub.org/public/igem/BBa_K346001_sequence/1

merR
type: SequenceAnnotation
persistent identity: annotation2093295
display id: annotation2093295
version: 1
top level: BBa_K346001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K346001/annotation2093295/range2093295/1

https://synbiohub.org/public/igem/BBa_K346001/annotation2093295/range2093295/1
type: Range
persistent identity: range2093295
display id: range2093295
version: 1
top level: BBa_K346001
direction: direction/forward
start: 19
end: 453
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2093291
display id: annotation2093291
version: 1
top level: BBa_K346001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K346001/annotation2093291/range2093291/1

https://synbiohub.org/public/igem/BBa_K346001/annotation2093291/range2093291/1
type: Range
persistent identity: range2093291
display id: range2093291
version: 1
top level: BBa_K346001
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_K1321344
type: ComponentDefinition
persistent identity: BBa K1321344
display id: BBa_K1321344
version: 1
was generated by: iGEM to SBOL conversion
description: NiBP fused to CBDcex in RFC 25
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:26:09Z
mutable provenance: asdf
owned by: james
owned by: myers
mutable description: asdf
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: asdf
star: false
creator: Chris N Micklem
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321009
component: BBa_B0105
component: BBa_K1321003
sequence annotation: BBa_K1321003
sequence annotation: BBa_K1321009
sequence annotation: BBa_B0105
sequence: https://synbiohub.org/public/igem/BBa_K1321344_sequence/1

BBa_K1321009
type: Component
persistent identity: component2415104
display id: component2415104
version: 1
top level: BBa_K1321344
access: public
definition: BBa_K1321009

BBa_B0105
type: Component
persistent identity: component2415106
display id: component2415106
version: 1
top level: BBa_K1321344
access: public
definition: Scar 25

BBa_K1321003
type: Component
persistent identity: component2415107
display id: component2415107
version: 1
top level: BBa_K1321344
access: public
definition: BBa_K1321003

BBa_K1321003
type: SequenceAnnotation
persistent identity: annotation2415107
display id: annotation2415107
version: 1
top level: BBa_K1321344
location: https://synbiohub.org/public/igem/BBa_K1321344/annotation2415107/range2415107/1
component: BBa_K1321003

https://synbiohub.org/public/igem/BBa_K1321344/annotation2415107/range2415107/1
type: Range
persistent identity: range2415107
display id: range2415107
version: 1
top level: BBa_K1321344
direction: direction/reverse
start: 183
end: 509
orientation: inline

BBa_K1321009
type: SequenceAnnotation
persistent identity: annotation2415104
display id: annotation2415104
version: 1
top level: BBa_K1321344
location: https://synbiohub.org/public/igem/BBa_K1321344/annotation2415104/range2415104/1
component: BBa_K1321009

https://synbiohub.org/public/igem/BBa_K1321344/annotation2415104/range2415104/1
type: Range
persistent identity: range2415104
display id: range2415104
version: 1
top level: BBa_K1321344
direction: direction/reverse
start: 1
end: 176
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2415106
display id: annotation2415106
version: 1
top level: BBa_K1321344
location: https://synbiohub.org/public/igem/BBa_K1321344/annotation2415106/range2415106/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321344/annotation2415106/range2415106/1
type: Range
persistent identity: range2415106
display id: range2415106
version: 1
top level: BBa_K1321344
direction: direction/reverse
start: 177
end: 182
orientation: inline

MymTsfGFP
type: ComponentDefinition
persistent identity: BBa K1980003
display id: BBa_K1980003
version: 1
was generated by: iGEM to SBOL conversion
description: MymT sfGFP
created: 2016-10-10T11:00:00Z
modified: 2016-10-20T07:26:41Z
mutable provenance: Mycobacterium tuberculosis. Ordered as codon optimised DNA.
owned by: james
owned by: myers
mutable description: MymT is a small prokaryotic copper metallothein discovered in Mycobacterium tuberculosis. It is believed that the protein may help the bacterium survive copper toxicity, though deleting the gene had no effect on pathogenicity in mice. It can bind up to 7 copper ions but has a preference for 4-6. This version has a C terminal sfGFP with a C terminal hexahistidine tag for purification. The MymT and sfGFP are separated by a small flexible linker. This part has been codon optimised for E. coli.
discontinued: false
dominant: false
experience: experience/Works
group u list: _2447_
m user id: 29607
owner id: 29607
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: This protein has a C terminal hexahistidine tag for purification and has been codon optimised for E. coli.
star: false
creator: Sam Garforth
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: linker
sequence annotation: sfGFP
sequence annotation: Double stop
sequence annotation: Hexahistidine
sequence annotation: MymT
sequence: https://synbiohub.org/public/igem/BBa_K1980003_sequence/1

linker
type: SequenceAnnotation
persistent identity: annotation2495244
display id: annotation2495244
version: 1
top level: MymTsfGFP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1980003/annotation2495244/range2495244/1

https://synbiohub.org/public/igem/BBa_K1980003/annotation2495244/range2495244/1
type: Range
persistent identity: range2495244
display id: range2495244
version: 1
top level: MymTsfGFP
direction: direction/forward
start: 160
end: 177
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2495245
display id: annotation2495245
version: 1
top level: MymTsfGFP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1980003/annotation2495245/range2495245/1

https://synbiohub.org/public/igem/BBa_K1980003/annotation2495245/range2495245/1
type: Range
persistent identity: range2495245
display id: range2495245
version: 1
top level: MymTsfGFP
direction: direction/forward
start: 178
end: 888
orientation: inline

Double stop
type: SequenceAnnotation
persistent identity: annotation2495248
display id: annotation2495248
version: 1
top level: MymTsfGFP
role: feature/stop
role: SO:0000319
location: https://synbiohub.org/public/igem/BBa_K1980003/annotation2495248/range2495248/1

https://synbiohub.org/public/igem/BBa_K1980003/annotation2495248/range2495248/1
type: Range
persistent identity: range2495248
display id: range2495248
version: 1
top level: MymTsfGFP
direction: direction/forward
start: 906
end: 912
orientation: inline

Hexahistidine
type: SequenceAnnotation
persistent identity: annotation2495246
display id: annotation2495246
version: 1
top level: MymTsfGFP
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1980003/annotation2495246/range2495246/1

https://synbiohub.org/public/igem/BBa_K1980003/annotation2495246/range2495246/1
type: Range
persistent identity: range2495246
display id: range2495246
version: 1
top level: MymTsfGFP
direction: direction/forward
start: 889
end: 906
orientation: inline

MymT
type: SequenceAnnotation
persistent identity: annotation2495243
display id: annotation2495243
version: 1
top level: MymTsfGFP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1980003/annotation2495243/range2495243/1

https://synbiohub.org/public/igem/BBa_K1980003/annotation2495243/range2495243/1
type: Range
persistent identity: range2495243
display id: range2495243
version: 1
top level: MymTsfGFP
direction: direction/forward
start: 1
end: 159
orientation: inline

BBa_K1321101
type: ComponentDefinition
persistent identity: BBa K1321101
display id: BBa_K1321101
version: 1
was generated by: iGEM to SBOL conversion
description: Phytochelatin (PC) EC20 fused to CBDclos with T7 promoter
created: 2014-10-08T11:00:00Z
modified: 2015-06-17T12:18:15Z
mutable provenance: PhytoC+CBD4
owned by: james
owned by: myers
mutable description: PhytoC+CBD4
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20780
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: PhytoC+CBD4
star: false
creator: Xenia Spencer-Milnes
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321002
component: BBa_K1319106
component: BBa_K1321005
component: BBa_B0105
component: BBa_K1321338
sequence annotation: BBa_K1321002
sequence annotation: BBa_B0105
sequence annotation: BBa_K1319106
sequence annotation: BBa_K1321005
sequence annotation: BBa_K1321338
sequence: https://synbiohub.org/public/igem/BBa_K1321101_sequence/1

BBa_K1321002
type: Component
persistent identity: component2415293
display id: component2415293
version: 1
top level: BBa_K1321101
access: public
definition: BBa_K1321002

BBa_K1319106
type: Component
persistent identity: component2415288
display id: component2415288
version: 1
top level: BBa_K1321101
access: public
definition: Scar25 ATG

BBa_K1321005
type: Component
persistent identity: component2415290
display id: component2415290
version: 1
top level: BBa_K1321101
access: public
definition: BBa_K1321005

BBa_B0105
type: Component
persistent identity: component2415292
display id: component2415292
version: 1
top level: BBa_K1321101
access: public
definition: Scar 25

BBa_K1321338
type: Component
persistent identity: component2415286
display id: component2415286
version: 1
top level: BBa_K1321101
access: public
definition: BBa_K1321338

BBa_K1321002
type: SequenceAnnotation
persistent identity: annotation2415293
display id: annotation2415293
version: 1
top level: BBa_K1321101
location: https://synbiohub.org/public/igem/BBa_K1321101/annotation2415293/range2415293/1
component: BBa_K1321002

https://synbiohub.org/public/igem/BBa_K1321101/annotation2415293/range2415293/1
type: Range
persistent identity: range2415293
display id: range2415293
version: 1
top level: BBa_K1321101
direction: direction/reverse
start: 188
end: 487
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2415292
display id: annotation2415292
version: 1
top level: BBa_K1321101
location: https://synbiohub.org/public/igem/BBa_K1321101/annotation2415292/range2415292/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321101/annotation2415292/range2415292/1
type: Range
persistent identity: range2415292
display id: range2415292
version: 1
top level: BBa_K1321101
direction: direction/reverse
start: 182
end: 187
orientation: inline

BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2415288
display id: annotation2415288
version: 1
top level: BBa_K1321101
location: https://synbiohub.org/public/igem/BBa_K1321101/annotation2415288/range2415288/1
component: BBa_K1319106

https://synbiohub.org/public/igem/BBa_K1321101/annotation2415288/range2415288/1
type: Range
persistent identity: range2415288
display id: range2415288
version: 1
top level: BBa_K1321101
direction: direction/reverse
start: 44
end: 58
orientation: inline

BBa_K1321005
type: SequenceAnnotation
persistent identity: annotation2415290
display id: annotation2415290
version: 1
top level: BBa_K1321101
location: https://synbiohub.org/public/igem/BBa_K1321101/annotation2415290/range2415290/1
component: BBa_K1321005

https://synbiohub.org/public/igem/BBa_K1321101/annotation2415290/range2415290/1
type: Range
persistent identity: range2415290
display id: range2415290
version: 1
top level: BBa_K1321101
direction: direction/reverse
start: 59
end: 181
orientation: inline

BBa_K1321338
type: SequenceAnnotation
persistent identity: annotation2415286
display id: annotation2415286
version: 1
top level: BBa_K1321101
location: https://synbiohub.org/public/igem/BBa_K1321101/annotation2415286/range2415286/1
component: BBa_K1321338

https://synbiohub.org/public/igem/BBa_K1321101/annotation2415286/range2415286/1
type: Range
persistent identity: range2415286
display id: range2415286
version: 1
top level: BBa_K1321101
direction: direction/reverse
start: 1
end: 43
orientation: inline

BBa_K205004
type: ComponentDefinition
persistent identity: BBa K205004
display id: BBa_K205004
version: 1
was generated by: iGEM to SBOL conversion
description: MerT - Membranous Mercury transporter
created: 2009-10-19T11:00:00Z
modified: 2016-01-28T12:25:29Z
mutable provenance: Sequenced sourced from Professor David Wilson, of the Department of Molecular Biology and Genetics, Cornell University.
owned by: james
owned by: myers
mutable description: MerT is a transporter responsible for the crossing of the inner membrane by mercury. It enhances mercury uptake from the environment.
discontinued: false
dominant: false
experience: experience/None
group u list: _311_
m user id: 4206
owner id: 4184
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Standard 23 prefix and suffix was added to the sequence.
star: false
creator: Katelin Haynes
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K205004_sequence/1

BBa_K1701000
type: ComponentDefinition
persistent identity: BBa K1701000
display id: BBa_K1701000
version: 1
was generated by: iGEM to SBOL conversion
description: GolB
created: 2015-09-07T11:00:00Z
modified: 2016-01-11T03:16:13Z
mutable provenance: http://www.ncbi.nlm.nih.gov/protein/NP_459350.1
owned by: james
owned by: myers
mutable description: Gold-specific binding protein (GolB) is a gold-binding chaperon discovered in S. Typhimurium which has been verified that the display of GolB on E.coli cell surface permitted selective enrichment of gold ions from media containing various metal ions.
discontinued: false
dominant: false
experience: experience/Works
group u list: _2119_
m user id: 0
m user id: 4206
owner id: 21319
owning group id: 9
sample status: In stock
status: status/Planning
status: status/Available
bookmark: false
mutable notes: None
star: false
creator: Wei Wei
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1701000_sequence/1

BBa_K643002
type: ComponentDefinition
persistent identity: BBa K643002
display id: BBa_K643002
version: 1
was generated by: iGEM to SBOL conversion
description: J140 metal binding peptide
created: 2011-09-27T11:00:00Z
modified: 2015-07-21T02:29:37Z
mutable provenance: This protein was selected from a pIII phage display library based on its ability to nucleate ZnS or CdS nanocrystals.
owned by: james
owned by: myers
mutable description: J140, with a sequence of Ser-Leu-Thr-Pro-Leu-Thr-Thr-Ser-His-Leu-Arg-Ser, has been shown to recognize single-crystal CdS and nucleate quantum dots (Mao, C., et al. "Viral assembly of oriented quantum dot nanowires" PNAS 100:6946, 2003)
discontinued: false
dominant: false
experience: experience/None
group u list: _819_
m user id: 4206
owner id: 9151
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: We wanted to be able to clone this part into the IPTG-inducible expression vector pET28b, so we engineered in restriction sites that would allow us to do so and keep everything in frame. Therefore the part contains an Nco1 site after the Xba1 site, and a BamH1 site before the Spe1 site.
star: false
creator: Rikki Frenkel, Justin Fabrikant
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K643002_sequence/1

CBDcex(T7)
type: ComponentDefinition
persistent identity: BBa K863103
display id: BBa_K863103
version: 1
was generated by: iGEM to SBOL conversion
description: Cellulose binding Domain from Cellulomonas Fimi with Reporter GFP
created: 2012-09-19T11:00:00Z
modified: 2015-06-17T01:19:26Z
mutable provenance: Cloning-vector and [http://partsregistry.org/Part:BBa_K863121 BBa_K863121]
owned by: james
owned by: myers
mutable description: Cellulose binding Domain of C. Fimi Exoglucanase gene with T7, RBS linked with two amino acids (GS) to a GFP (Freiburg-Standard)
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 4206
owner id: 13080
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: CBDcex fused to a his-tagged GFP in a Freiburg-manner
star: false
creator: Moritz M??ller
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Exoglucanase
sequence annotation: TAA
sequence annotation: BBa_K863102
sequence annotation: BBa_K525998
sequence annotation: His-Tag
sequence annotation: Cellulose binding domain (CBDcex)
sequence annotation: ATG
sequence annotation: Freiburg-Scar
sequence annotation: T7 promoter
sequence annotation: B0034
sequence annotation: AgeI
sequence annotation: BBa_K863101
sequence annotation: BBa_K863121
sequence annotation: RBS
sequence annotation: BBa_E0040
sequence annotation: GS-Linker
sequence: https://synbiohub.org/public/igem/BBa_K863103_sequence/1

Exoglucanase
type: SequenceAnnotation
persistent identity: annotation2188021
display id: annotation2188021
version: 1
top level: CBDcex(T7)
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188021/range2188021/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188021/range2188021/1
type: Range
persistent identity: range2188021
display id: range2188021
version: 1
top level: CBDcex(T7)
direction: direction/forward
start: 350
end: 358
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2188028
display id: annotation2188028
version: 1
top level: CBDcex(T7)
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188028/range2188028/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188028/range2188028/1
type: Range
persistent identity: range2188028
display id: range2188028
version: 1
top level: CBDcex(T7)
direction: direction/forward
start: 1109
end: 1111
orientation: inline

BBa_K863102
type: SequenceAnnotation
persistent identity: annotation2188014
display id: annotation2188014
version: 1
top level: CBDcex(T7)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188014/range2188014/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188014/range2188014/1
type: Range
persistent identity: range2188014
display id: range2188014
version: 1
top level: CBDcex(T7)
direction: direction/forward
start: 1
end: 364
orientation: inline

BBa_K525998
type: SequenceAnnotation
persistent identity: annotation2188013
display id: annotation2188013
version: 1
top level: CBDcex(T7)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188013/range2188013/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188013/range2188013/1
type: Range
persistent identity: range2188013
display id: range2188013
version: 1
top level: CBDcex(T7)
direction: direction/forward
start: 1
end: 32
orientation: inline

His-Tag
type: SequenceAnnotation
persistent identity: annotation2188026
display id: annotation2188026
version: 1
top level: CBDcex(T7)
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188026/range2188026/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188026/range2188026/1
type: Range
persistent identity: range2188026
display id: range2188026
version: 1
top level: CBDcex(T7)
direction: direction/forward
start: 1085
end: 1102
orientation: inline

Cellulose binding domain (CBDcex)
type: SequenceAnnotation
persistent identity: annotation2188020
display id: annotation2188020
version: 1
top level: CBDcex(T7)
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188020/range2188020/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188020/range2188020/1
type: Range
persistent identity: range2188020
display id: range2188020
version: 1
top level: CBDcex(T7)
direction: direction/forward
start: 50
end: 349
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2188017
display id: annotation2188017
version: 1
top level: CBDcex(T7)
role: feature/start
role: SO:0000318
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188017/range2188017/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188017/range2188017/1
type: Range
persistent identity: range2188017
display id: range2188017
version: 1
top level: CBDcex(T7)
direction: direction/forward
start: 35
end: 37
orientation: inline

Freiburg-Scar
type: SequenceAnnotation
persistent identity: annotation2188023
display id: annotation2188023
version: 1
top level: CBDcex(T7)
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188023/range2188023/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188023/range2188023/1
type: Range
persistent identity: range2188023
display id: range2188023
version: 1
top level: CBDcex(T7)
direction: direction/forward
start: 365
end: 370
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2188012
display id: annotation2188012
version: 1
top level: CBDcex(T7)
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188012/range2188012/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188012/range2188012/1
type: Range
persistent identity: range2188012
display id: range2188012
version: 1
top level: CBDcex(T7)
direction: direction/forward
start: 1
end: 20
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2188015
display id: annotation2188015
version: 1
top level: CBDcex(T7)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188015/range2188015/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188015/range2188015/1
type: Range
persistent identity: range2188015
display id: range2188015
version: 1
top level: CBDcex(T7)
direction: direction/forward
start: 21
end: 32
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2188027
display id: annotation2188027
version: 1
top level: CBDcex(T7)
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188027/range2188027/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188027/range2188027/1
type: Range
persistent identity: range2188027
display id: range2188027
version: 1
top level: CBDcex(T7)
direction: direction/forward
start: 1103
end: 1108
orientation: inline

BBa_K863101
type: SequenceAnnotation
persistent identity: annotation2188019
display id: annotation2188019
version: 1
top level: CBDcex(T7)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188019/range2188019/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188019/range2188019/1
type: Range
persistent identity: range2188019
display id: range2188019
version: 1
top level: CBDcex(T7)
direction: direction/forward
start: 38
end: 364
orientation: inline

BBa_K863121
type: SequenceAnnotation
persistent identity: annotation2188025
display id: annotation2188025
version: 1
top level: CBDcex(T7)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188025/range2188025/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188025/range2188025/1
type: Range
persistent identity: range2188025
display id: range2188025
version: 1
top level: CBDcex(T7)
direction: direction/forward
start: 371
end: 1111
orientation: inline

Exoglucanase
type: SequenceAnnotation
persistent identity: annotation2188018
display id: annotation2188018
version: 1
top level: CBDcex(T7)
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188018/range2188018/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188018/range2188018/1
type: Range
persistent identity: range2188018
display id: range2188018
version: 1
top level: CBDcex(T7)
direction: direction/forward
start: 38
end: 49
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2188016
display id: annotation2188016
version: 1
top level: CBDcex(T7)
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188016/range2188016/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188016/range2188016/1
type: Range
persistent identity: range2188016
display id: range2188016
version: 1
top level: CBDcex(T7)
direction: direction/forward
start: 24
end: 27
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2188024
display id: annotation2188024
version: 1
top level: CBDcex(T7)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188024/range2188024/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188024/range2188024/1
type: Range
persistent identity: range2188024
display id: range2188024
version: 1
top level: CBDcex(T7)
direction: direction/forward
start: 371
end: 1084
orientation: inline

GS-Linker
type: SequenceAnnotation
persistent identity: annotation2188022
display id: annotation2188022
version: 1
top level: CBDcex(T7)
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188022/range2188022/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188022/range2188022/1
type: Range
persistent identity: range2188022
display id: range2188022
version: 1
top level: CBDcex(T7)
direction: direction/forward
start: 359
end: 364
orientation: inline

BBa_K1478001
type: ComponentDefinition
persistent identity: BBa K1478001
display id: BBa_K1478001
version: 1
was generated by: iGEM to SBOL conversion
description: Cellulose binding domain
created: 2014-09-12T11:00:00Z
modified: 2015-06-17T01:26:18Z
mutable provenance: Domain derived from cellulose binding protein from Chlostridium cellulovorans(WP_013291799).
owned by: james
owned by: myers
mutable description: Coding region for cellulose binding domain. When fused to a protein, protein attaches to cellulose
discontinued: false
dominant: false
group u list: _1858_
m user id: 4206
owner id: 20973
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Sequence was optimized for Escherichia coli and Arabidopsis thaliana codon usage.
star: false
creator: Fabian Fr??mling
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K1478001_sequence/1

BBa_K1550004
type: ComponentDefinition
persistent identity: BBa K1550004
display id: BBa_K1550004
version: 1
was generated by: iGEM to SBOL conversion
description: BmtA (Lead/Zinc-binding metallothionein from Oscillatoria brevis, codon optimized for E. coli)
created: 2014-10-16T11:00:00Z
modified: 2015-08-03T03:05:43Z
mutable provenance: The sequence used for the BmtA (metallothionein) was codon optimized for E. coli (using the browser-based software, Gene Design). The native sequence comes from Oscillatoria brevis, and was sourced from an entry in the NCBI database by Liu,T., Nakashima,S., Hirose,K., Uemura,Y., Shibasaka,M., Katsuhara,M. and Kasamo,K. (2003).
owned by: james
owned by: myers
mutable description: This is the BmtA coding sequence from Oscillatoria brevis, which encodes lead and zinc binding metallothionein (Liu et al., 2003). The BmtA sequence in this part has been codon optimized for E. coli. This part can be used with a constitutive or inducible promoter to produce metallothionein in projects utilizing the metal-binding protein for sequestration purposes.
discontinued: false
dominant: false
group u list: _1934_
m user id: 4206
owner id: 23749
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: When used in E. coli for the sequestration of lead or zinc ions in solution, the part may or may not work better in concert with a genomic ZntA knockout. We plan to characterize this combination in the near future.
star: false
creator: Jesse Goldenberg
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1550004_sequence/1

BBa_K1321345
type: ComponentDefinition
persistent identity: BBa K1321345
display id: BBa_K1321345
version: 1
was generated by: iGEM to SBOL conversion
description: NiBP fused to dCBD with linker in RFC 25
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:25:54Z
mutable provenance: asdf
owned by: james
owned by: myers
mutable description: asdf
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: asdf
star: false
creator: Chris N Micklem
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_B0105
component: BBa_K1321340
component: BBa_K1321009
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321340
sequence annotation: BBa_K1321009
sequence: https://synbiohub.org/public/igem/BBa_K1321345_sequence/1

BBa_B0105
type: Component
persistent identity: component2415112
display id: component2415112
version: 1
top level: BBa_K1321345
access: public
definition: Scar 25

BBa_K1321340
type: Component
persistent identity: component2415114
display id: component2415114
version: 1
top level: BBa_K1321345
access: public
definition: BBa_K1321340

BBa_K1321009
type: Component
persistent identity: component2415110
display id: component2415110
version: 1
top level: BBa_K1321345
access: public
definition: BBa_K1321009

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2415112
display id: annotation2415112
version: 1
top level: BBa_K1321345
location: https://synbiohub.org/public/igem/BBa_K1321345/annotation2415112/range2415112/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321345/annotation2415112/range2415112/1
type: Range
persistent identity: range2415112
display id: range2415112
version: 1
top level: BBa_K1321345
direction: direction/reverse
start: 177
end: 182
orientation: inline

BBa_K1321340
type: SequenceAnnotation
persistent identity: annotation2415114
display id: annotation2415114
version: 1
top level: BBa_K1321345
location: https://synbiohub.org/public/igem/BBa_K1321345/annotation2415114/range2415114/1
component: BBa_K1321340

https://synbiohub.org/public/igem/BBa_K1321345/annotation2415114/range2415114/1
type: Range
persistent identity: range2415114
display id: range2415114
version: 1
top level: BBa_K1321345
direction: direction/reverse
start: 183
end: 548
orientation: inline

BBa_K1321009
type: SequenceAnnotation
persistent identity: annotation2415110
display id: annotation2415110
version: 1
top level: BBa_K1321345
location: https://synbiohub.org/public/igem/BBa_K1321345/annotation2415110/range2415110/1
component: BBa_K1321009

https://synbiohub.org/public/igem/BBa_K1321345/annotation2415110/range2415110/1
type: Range
persistent identity: range2415110
display id: range2415110
version: 1
top level: BBa_K1321345
direction: direction/reverse
start: 1
end: 176
orientation: inline

CBDcex
type: ComponentDefinition
persistent identity: BBa K863101
display id: BBa_K863101
version: 1
was generated by: iGEM to SBOL conversion
description: Cellulose binding Domain of Cellulomonas Fimi Exoglucanse (Freiburg-Standard)
created: 2012-09-17T11:00:00Z
modified: 2015-06-17T01:18:43Z
mutable provenance: Cloning-Vector with a CBDcex-Barnase fusion-protein
owned by: james
owned by: myers
mutable description: Cellulose binding domain of the Cellulomonas fimi ATCC 484 exoglucanase gene
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 4206
owner id: 13080
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The sequence starts with the Freiburg-prefix (ATG+NgoMIV-restrictionsite) followed by 12 Bases (4 AS) of the exoglucanase gene in front of the CBD. Then 9 bases of the exoglucanase gene behind the CBD. After that the sequence continues with a short GS-Linker.
star: false
creator: Moritz M??ller
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: TAA
sequence annotation: NgoMIV
sequence annotation: GS-Linker
sequence annotation: AgeI
sequence annotation: Cellulose binding domain (CBDcex)
sequence annotation: Exoglucanase
sequence annotation: ATG
sequence: https://synbiohub.org/public/igem/BBa_K863101_sequence/1

TAA
type: SequenceAnnotation
persistent identity: annotation2184934
display id: annotation2184934
version: 1
top level: CBDcex
role: feature/stop
role: SO:0000319
location: https://synbiohub.org/public/igem/BBa_K863101/annotation2184934/range2184934/1

https://synbiohub.org/public/igem/BBa_K863101/annotation2184934/range2184934/1
type: Range
persistent identity: range2184934
display id: range2184934
version: 1
top level: CBDcex
direction: direction/forward
start: 343
end: 345
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2184939
display id: annotation2184939
version: 1
top level: CBDcex
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863101/annotation2184939/range2184939/1

https://synbiohub.org/public/igem/BBa_K863101/annotation2184939/range2184939/1
type: Range
persistent identity: range2184939
display id: range2184939
version: 1
top level: CBDcex
direction: direction/forward
start: 4
end: 9
orientation: inline

GS-Linker
type: SequenceAnnotation
persistent identity: annotation2184938
display id: annotation2184938
version: 1
top level: CBDcex
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863101/annotation2184938/range2184938/1

https://synbiohub.org/public/igem/BBa_K863101/annotation2184938/range2184938/1
type: Range
persistent identity: range2184938
display id: range2184938
version: 1
top level: CBDcex
direction: direction/forward
start: 331
end: 336
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2184940
display id: annotation2184940
version: 1
top level: CBDcex
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863101/annotation2184940/range2184940/1

https://synbiohub.org/public/igem/BBa_K863101/annotation2184940/range2184940/1
type: Range
persistent identity: range2184940
display id: range2184940
version: 1
top level: CBDcex
direction: direction/forward
start: 337
end: 342
orientation: inline

Cellulose binding domain (CBDcex)
type: SequenceAnnotation
persistent identity: annotation2184935
display id: annotation2184935
version: 1
top level: CBDcex
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K863101/annotation2184935/range2184935/1

https://synbiohub.org/public/igem/BBa_K863101/annotation2184935/range2184935/1
type: Range
persistent identity: range2184935
display id: range2184935
version: 1
top level: CBDcex
direction: direction/forward
start: 22
end: 321
orientation: inline

Exoglucanase
type: SequenceAnnotation
persistent identity: annotation2184937
display id: annotation2184937
version: 1
top level: CBDcex
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863101/annotation2184937/range2184937/1

https://synbiohub.org/public/igem/BBa_K863101/annotation2184937/range2184937/1
type: Range
persistent identity: range2184937
display id: range2184937
version: 1
top level: CBDcex
direction: direction/forward
start: 322
end: 330
orientation: inline

Exoglucanase
type: SequenceAnnotation
persistent identity: annotation2184936
display id: annotation2184936
version: 1
top level: CBDcex
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863101/annotation2184936/range2184936/1

https://synbiohub.org/public/igem/BBa_K863101/annotation2184936/range2184936/1
type: Range
persistent identity: range2184936
display id: range2184936
version: 1
top level: CBDcex
direction: direction/forward
start: 10
end: 21
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2184933
display id: annotation2184933
version: 1
top level: CBDcex
role: feature/start
role: SO:0000318
location: https://synbiohub.org/public/igem/BBa_K863101/annotation2184933/range2184933/1

https://synbiohub.org/public/igem/BBa_K863101/annotation2184933/range2184933/1
type: Range
persistent identity: range2184933
display id: range2184933
version: 1
top level: CBDcex
direction: direction/forward
start: 1
end: 3
orientation: inline

BBa_K1321005
type: ComponentDefinition
persistent identity: BBa K1321005
display id: BBa_K1321005
version: 1
was generated by: iGEM to SBOL conversion
description: Synthetic Phytochelatin EC(20) RFC[25]
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:09:51Z
mutable provenance: Sequence is not natural. Basis for the sequence was from the following reference: Bae W, Chen W, Mulchandani A, Mehra RK (2000) Enhanced bioaccumulation of heavy metals by bacterial cells displaying synthetic phytochelatins. Biotechnol Bioeng. 70(5):518-24.
owned by: james
owned by: myers
mutable description: Synthetic Phytochelatin EC(20) rfc25 fusion format
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 0
owner id: 20780
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: Codon optimised for E.coli.
star: false
creator: Xenia Spencer-Milnes
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Synthetic Phytochelatin EC(20)
sequence: https://synbiohub.org/public/igem/BBa_K1321005_sequence/1

Synthetic Phytochelatin EC(20)
type: SequenceAnnotation
persistent identity: annotation2414598
display id: annotation2414598
version: 1
top level: BBa_K1321005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1321005/annotation2414598/range2414598/1

https://synbiohub.org/public/igem/BBa_K1321005/annotation2414598/range2414598/1
type: Range
persistent identity: range2414598
display id: range2414598
version: 1
top level: BBa_K1321005
direction: direction/forward
start: 1
end: 123
orientation: inline

BBa_K1505001
type: ComponentDefinition
persistent identity: BBa K1505001
display id: BBa_K1505001
version: 1
was generated by: iGEM to SBOL conversion
description: RBS+smtA+mCherry
created: 2014-10-09T11:00:00Z
modified: 2016-01-13T12:56:38Z
mutable provenance: RBS is predicted from pmsp3 promoter from Magnetospirillum magneticum strain AMB-1 genome. smtA sequence is from BioBrick part BBa_K190021 (we modified the illegal PstI site).
owned by: james
owned by: myers
mutable description: The RBS is from pmsp3 promoter, the strongest promoter isolated from Magnetospirillum bacteria. SmtA is a metallithionein isolated from E.coli and it binds covalently to divalent metal cation. It can usually binds 3-4 ions per protein, depending on the ion types. It is often used to increase the accumulation of metal ions intracellularly and potentially improve bacteria's tolerance of metal ions. The SmtA protein and mCherry protein are linked by a fusion protein linker. The purpose of the linkage is to improve the expression/folding of SmtA and for better visualization of successful transformation. The original use is to express SmtA in Magnetospirillum magneticum strain AMB-1.
discontinued: false
dominant: false
experience: experience/None
group u list: _1885_
m user id: 4206
owner id: 22465
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: We researched the optimum linker sequence for fusion protein to facilitate successful fusion. The mCherry protein is expected to improve the folding of SmtA (from E.coli) in AMB-1.
star: false
creator: Renyao Wei
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: SmtA
sequence annotation: ggghs linker
sequence annotation: mCherry
sequence annotation: rbs (from pmsp3)
sequence: https://synbiohub.org/public/igem/BBa_K1505001_sequence/1

SmtA
type: SequenceAnnotation
persistent identity: annotation2412324
display id: annotation2412324
version: 1
top level: BBa_K1505001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1505001/annotation2412324/range2412324/1

https://synbiohub.org/public/igem/BBa_K1505001/annotation2412324/range2412324/1
type: Range
persistent identity: range2412324
display id: range2412324
version: 1
top level: BBa_K1505001
direction: direction/forward
start: 66
end: 233
orientation: inline

ggghs linker
type: SequenceAnnotation
persistent identity: annotation2412331
display id: annotation2412331
version: 1
top level: BBa_K1505001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1505001/annotation2412331/range2412331/1

https://synbiohub.org/public/igem/BBa_K1505001/annotation2412331/range2412331/1
type: Range
persistent identity: range2412331
display id: range2412331
version: 1
top level: BBa_K1505001
direction: direction/forward
start: 234
end: 248
orientation: inline

mCherry
type: SequenceAnnotation
persistent identity: annotation2412326
display id: annotation2412326
version: 1
top level: BBa_K1505001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1505001/annotation2412326/range2412326/1

https://synbiohub.org/public/igem/BBa_K1505001/annotation2412326/range2412326/1
type: Range
persistent identity: range2412326
display id: range2412326
version: 1
top level: BBa_K1505001
direction: direction/forward
start: 249
end: 959
orientation: inline

rbs (from pmsp3)
type: SequenceAnnotation
persistent identity: annotation2412325
display id: annotation2412325
version: 1
top level: BBa_K1505001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1505001/annotation2412325/range2412325/1

https://synbiohub.org/public/igem/BBa_K1505001/annotation2412325/range2412325/1
type: Range
persistent identity: range2412325
display id: range2412325
version: 1
top level: BBa_K1505001
direction: direction/forward
start: 20
end: 47
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: RBS
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_K1460001
type: ComponentDefinition
persistent identity: BBa K1460001
display id: BBa_K1460001
version: 1
was generated by: iGEM to SBOL conversion
description: pT7 + RBS + GST (glutathione-S-transferase)-CRS5 (metallothionein) + Ter
created: 2014-08-13T11:00:00Z
modified: 2016-12-03T10:52:20Z
mutable provenance: T7 promoter is part BBa_I712074. RBS is from part BBa_J61101. Glutathione-S-transferase is from S. japonicum. CRS5 is a metallothionein from S. cerevisiae. Terminator is part BBa_B1006. This sequence was synthesized by GenScript in the vector pUC57 and was then transferred using traditional cloning into pSB1C3.
owned by: james
owned by: myers
mutable description: This part serves as a functional metallothionein for heavy metal binding and conferred resistance to heavy metals when combined with a strain expressing the bacteriophage T7 polymerase. CRS5 gene is the metallothionein that binds to heavy metals and it is fused to GST for stability.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1839_
m user id: 24251
owner id: 15649
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: Flanking the T7 promoter are two KpnI cut sites with both the T7 promoter and a SacI cut site between them. These cut sites are present for easy removal of the T7 promoter for modulation of parts with other promoters. The SacI cut site can be used as a marker for successful removal of the T7 promoter from the part. When digested with SacI, part BBa_K1460001 in pSB1C3 will yield bands of size 2166bp and 931bp. If the T7 promoter is successfully removed, when digested with SacI the plasmid will yield the linearized length of the pSB1C3+BBa_K1460001
star: false
creator: Eric Holmes
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: Linker Sequence
sequence annotation: BBa_J61101
sequence annotation: GST
sequence annotation: BBa_B1006
sequence annotation: T7 Promoter
sequence annotation: CRS5
sequence: https://synbiohub.org/public/igem/BBa_K1460001_sequence/1

Linker Sequence
type: SequenceAnnotation
persistent identity: annotation2380963
display id: annotation2380963
version: 1
top level: BBa_K1460001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1460001/annotation2380963/range2380963/1

https://synbiohub.org/public/igem/BBa_K1460001/annotation2380963/range2380963/1
type: Range
persistent identity: range2380963
display id: range2380963
version: 1
top level: BBa_K1460001
direction: direction/forward
start: 746
end: 778
orientation: inline

BBa_J61101
type: SequenceAnnotation
persistent identity: annotation2380961
display id: annotation2380961
version: 1
top level: BBa_K1460001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1460001/annotation2380961/range2380961/1

https://synbiohub.org/public/igem/BBa_K1460001/annotation2380961/range2380961/1
type: Range
persistent identity: range2380961
display id: range2380961
version: 1
top level: BBa_K1460001
direction: direction/forward
start: 72
end: 85
orientation: inline

GST
type: SequenceAnnotation
persistent identity: annotation2380962
display id: annotation2380962
version: 1
top level: BBa_K1460001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1460001/annotation2380962/range2380962/1

https://synbiohub.org/public/igem/BBa_K1460001/annotation2380962/range2380962/1
type: Range
persistent identity: range2380962
display id: range2380962
version: 1
top level: BBa_K1460001
direction: direction/forward
start: 92
end: 745
orientation: inline

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2380965
display id: annotation2380965
version: 1
top level: BBa_K1460001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1460001/annotation2380965/range2380965/1

https://synbiohub.org/public/igem/BBa_K1460001/annotation2380965/range2380965/1
type: Range
persistent identity: range2380965
display id: range2380965
version: 1
top level: BBa_K1460001
direction: direction/forward
start: 989
end: 1027
orientation: inline

T7 Promoter
type: SequenceAnnotation
persistent identity: annotation2380960
display id: annotation2380960
version: 1
top level: BBa_K1460001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1460001/annotation2380960/range2380960/1

https://synbiohub.org/public/igem/BBa_K1460001/annotation2380960/range2380960/1
type: Range
persistent identity: range2380960
display id: range2380960
version: 1
top level: BBa_K1460001
direction: direction/forward
start: 13
end: 59
orientation: inline

CRS5
type: SequenceAnnotation
persistent identity: annotation2380964
display id: annotation2380964
version: 1
top level: BBa_K1460001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1460001/annotation2380964/range2380964/1

https://synbiohub.org/public/igem/BBa_K1460001/annotation2380964/range2380964/1
type: Range
persistent identity: range2380964
display id: range2380964
version: 1
top level: BBa_K1460001
direction: direction/forward
start: 779
end: 988
orientation: inline

BBa_K1321002
type: ComponentDefinition
persistent identity: BBa K1321002
display id: BBa_K1321002
version: 1
was generated by: iGEM to SBOL conversion
description: Re-entry of BBa_K863111, Cellulose Binding Domain CBDclos in rfc25
created: 2014-10-09T11:00:00Z
modified: 2015-06-17T12:35:41Z
mutable provenance: This is a direct copy of BBa_K863111 with slight modifications to the beginning and end of the sequence for the purposes of allowing composite parts to be added correctly using this part.
owned by: james
owned by: myers
mutable description: This part is a copy of BBa_K863111 (Cellulose binding domain of C. cellulovorans cellulose binding protein gene (Freiburg-Standard)) and was created only as a registry entry, removing the atg-NgoMIV parts at the front and the AgeI-taa basepairs from the sequence so that the registry does not flag it as rfc25 incompatible, and it enables us to enter fusion proteins with this part as automatic composite parts in the method as described by: http://2009.igem.org/Team:Freiburg_bioware/general.
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
owner id: 20780
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This is a direct copy of BBa_K863111 with slight modifications to the beginning and end of the sequence for the purposes of allowing composite parts to be added correctly using this part.
star: false
creator: Xenia Spencer-Milnes
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1321002_sequence/1

BBa_K1321110
type: ComponentDefinition
persistent identity: BBa K1321110
display id: BBa_K1321110
version: 1
was generated by: iGEM to SBOL conversion
description: Phytochelatin (PC) EC20 fused to linker-dCBD with LacI
created: 2014-10-08T11:00:00Z
modified: 2015-06-17T12:19:00Z
mutable provenance: Phytochelatin EC(20) +CBD2 with LacI
owned by: james
owned by: myers
mutable description: Phytochelatin EC(20) +CBD2 with LacI
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20780
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Phytochelatin EC(20) +CBD2 with LacI
star: false
creator: Xenia Spencer-Milnes
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321340
component: BBa_K1319106
component: BBa_K1321005
component: BBa_J04500
component: BBa_B0105
sequence annotation: BBa_J04500
sequence annotation: BBa_K1321005
sequence annotation: BBa_K1321340
sequence annotation: BBa_B0105
sequence annotation: BBa_K1319106
sequence: https://synbiohub.org/public/igem/BBa_K1321110_sequence/1

BBa_K1321340
type: Component
persistent identity: component2414870
display id: component2414870
version: 1
top level: BBa_K1321110
access: public
definition: BBa_K1321340

BBa_K1319106
type: Component
persistent identity: component2414864
display id: component2414864
version: 1
top level: BBa_K1321110
access: public
definition: Scar25 ATG

BBa_K1321005
type: Component
persistent identity: component2414866
display id: component2414866
version: 1
top level: BBa_K1321110
access: public
definition: BBa_K1321005

BBa_J04500
type: Component
persistent identity: component2414862
display id: component2414862
version: 1
top level: BBa_K1321110
access: public
definition: BBa_J04500

BBa_B0105
type: Component
persistent identity: component2414868
display id: component2414868
version: 1
top level: BBa_K1321110
access: public
definition: Scar 25

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2414862
display id: annotation2414862
version: 1
top level: BBa_K1321110
location: https://synbiohub.org/public/igem/BBa_K1321110/annotation2414862/range2414862/1
component: BBa_J04500

https://synbiohub.org/public/igem/BBa_K1321110/annotation2414862/range2414862/1
type: Range
persistent identity: range2414862
display id: range2414862
version: 1
top level: BBa_K1321110
direction: direction/reverse
start: 1
end: 220
orientation: inline

BBa_K1321005
type: SequenceAnnotation
persistent identity: annotation2414866
display id: annotation2414866
version: 1
top level: BBa_K1321110
location: https://synbiohub.org/public/igem/BBa_K1321110/annotation2414866/range2414866/1
component: BBa_K1321005

https://synbiohub.org/public/igem/BBa_K1321110/annotation2414866/range2414866/1
type: Range
persistent identity: range2414866
display id: range2414866
version: 1
top level: BBa_K1321110
direction: direction/reverse
start: 236
end: 358
orientation: inline

BBa_K1321340
type: SequenceAnnotation
persistent identity: annotation2414870
display id: annotation2414870
version: 1
top level: BBa_K1321110
location: https://synbiohub.org/public/igem/BBa_K1321110/annotation2414870/range2414870/1
component: BBa_K1321340

https://synbiohub.org/public/igem/BBa_K1321110/annotation2414870/range2414870/1
type: Range
persistent identity: range2414870
display id: range2414870
version: 1
top level: BBa_K1321110
direction: direction/reverse
start: 365
end: 730
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2414868
display id: annotation2414868
version: 1
top level: BBa_K1321110
location: https://synbiohub.org/public/igem/BBa_K1321110/annotation2414868/range2414868/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321110/annotation2414868/range2414868/1
type: Range
persistent identity: range2414868
display id: range2414868
version: 1
top level: BBa_K1321110
direction: direction/reverse
start: 359
end: 364
orientation: inline

BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2414864
display id: annotation2414864
version: 1
top level: BBa_K1321110
location: https://synbiohub.org/public/igem/BBa_K1321110/annotation2414864/range2414864/1
component: BBa_K1319106

https://synbiohub.org/public/igem/BBa_K1321110/annotation2414864/range2414864/1
type: Range
persistent identity: range2414864
display id: range2414864
version: 1
top level: BBa_K1321110
direction: direction/reverse
start: 221
end: 235
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem_loop
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_K1933103
type: ComponentDefinition
persistent identity: BBa K1933103
display id: BBa_K1933103
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive expression of CBDclos fused to BclA with 6xHis tag
created: 2016-10-09T11:00:00Z
modified: 2016-10-18T11:05:25Z
mutable provenance: The BclA surface expression component derives from Bacillus anthracis(34F2), it is a hair-like protein that covers B. anthracis??? spores. We used parts BBa_K1933000 from iGEMKyoto 2016 for construction. We outsourced chemical synthesis of this part for construction. CBDclos derives from Clostridium cellulovorans, and is part of a multi-unit cellulosomes. We used parts BBa_K1321341 from iGEM Imperial 2014 for construction.
owned by: james
owned by: myers
mutable description: CBDclos fused to BclA with 6xHis tag is one of a series of surface expressing fusion proteins that make up biodevice that aims to be therapeutic solution against norovirus infections. This protein in particular is a cellulose binding protein(CBD) fused to surface expression anchoring domain(BclA), connected by a 6xHis tag to be easily identified by Western blotting. For more information, please visit our Wiki page.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2400_
m user id: 23701
owner id: 29998
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: The stop codon for this part is not in the coding region, but in the suffix region.
star: false
creator: Tomoki Uchino
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1933004
component: BBa_K1933000
component: BBa_K1321002
sequence annotation: BBa_K1933004
sequence annotation: BBa_K1933000
sequence annotation: BBa_K1321002
sequence: https://synbiohub.org/public/igem/BBa_K1933103_sequence/1

BBa_K1933004
type: Component
persistent identity: component2490476
display id: component2490476
version: 1
top level: BBa_K1933103
access: public
definition: BBa_K1933004

BBa_K1933000
type: Component
persistent identity: component2490480
display id: component2490480
version: 1
top level: BBa_K1933103
access: public
definition: BclA-His

BBa_K1321002
type: Component
persistent identity: component2490481
display id: component2490481
version: 1
top level: BBa_K1933103
access: public
definition: BBa_K1321002

BBa_K1933004
type: SequenceAnnotation
persistent identity: annotation2490476
display id: annotation2490476
version: 1
top level: BBa_K1933103
location: https://synbiohub.org/public/igem/BBa_K1933103/annotation2490476/range2490476/1
component: BBa_K1933004

https://synbiohub.org/public/igem/BBa_K1933103/annotation2490476/range2490476/1
type: Range
persistent identity: range2490476
display id: range2490476
version: 1
top level: BBa_K1933103
direction: direction/reverse
start: 1
end: 60
orientation: inline

BBa_K1933000
type: SequenceAnnotation
persistent identity: annotation2490480
display id: annotation2490480
version: 1
top level: BBa_K1933103
location: https://synbiohub.org/public/igem/BBa_K1933103/annotation2490480/range2490480/1
component: BBa_K1933000

https://synbiohub.org/public/igem/BBa_K1933103/annotation2490480/range2490480/1
type: Range
persistent identity: range2490480
display id: range2490480
version: 1
top level: BBa_K1933103
direction: direction/reverse
start: 61
end: 144
orientation: inline

BBa_K1321002
type: SequenceAnnotation
persistent identity: annotation2490481
display id: annotation2490481
version: 1
top level: BBa_K1933103
location: https://synbiohub.org/public/igem/BBa_K1933103/annotation2490481/range2490481/1
component: BBa_K1321002

https://synbiohub.org/public/igem/BBa_K1933103/annotation2490481/range2490481/1
type: Range
persistent identity: range2490481
display id: range2490481
version: 1
top level: BBa_K1933103
direction: direction/reverse
start: 145
end: 444
orientation: inline

BBa_K346004
type: ComponentDefinition
persistent identity: BBa K346004
display id: BBa_K346004
version: 1
was generated by: iGEM to SBOL conversion
description: RBS(B0034)_MBP(lead metal binding peptide egineered from PbrR)+Terminator(B0015)
created: 2010-10-13T11:00:00Z
modified: 2016-01-13T10:29:19Z
mutable provenance: yes
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This part is combined with a rbs, a mbp which codes for the metal binding peptide and a terminator.
discontinued: false
dominant: false
experience: experience/Works
group u list: _457_
m user id: 4206
owner id: 6045
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: to be given
star: false
creator: Junyi Jiao
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_B0015
sequence annotation: mbp(lead binding peptide)
sequence annotation: conserved
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K346004_sequence/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2100341
display id: annotation2100341
version: 1
top level: BBa_K346004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K346004/annotation2100341/range2100341/1

https://synbiohub.org/public/igem/BBa_K346004/annotation2100341/range2100341/1
type: Range
persistent identity: range2100341
display id: range2100341
version: 1
top level: BBa_K346004
direction: direction/forward
start: 351
end: 479
orientation: inline

mbp(lead binding peptide)
type: SequenceAnnotation
persistent identity: annotation2100340
display id: annotation2100340
version: 1
top level: BBa_K346004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K346004/annotation2100340/range2100340/1

https://synbiohub.org/public/igem/BBa_K346004/annotation2100340/range2100340/1
type: Range
persistent identity: range2100340
display id: range2100340
version: 1
top level: BBa_K346004
direction: direction/reverse
start: 19
end: 345
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2100339
display id: annotation2100339
version: 1
top level: BBa_K346004
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K346004/annotation2100339/range2100339/1

https://synbiohub.org/public/igem/BBa_K346004/annotation2100339/range2100339/1
type: Range
persistent identity: range2100339
display id: range2100339
version: 1
top level: BBa_K346004
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2100338
display id: annotation2100338
version: 1
top level: BBa_K346004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K346004/annotation2100338/range2100338/1

https://synbiohub.org/public/igem/BBa_K346004/annotation2100338/range2100338/1
type: Range
persistent identity: range2100338
display id: range2100338
version: 1
top level: BBa_K346004
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_K1980010
type: ComponentDefinition
persistent identity: BBa K1980010
display id: BBa_K1980010
version: 1
was generated by: iGEM to SBOL conversion
description: pCopA TAT Csp1 sfGFP with divergent CueR
created: 2016-10-10T11:00:00Z
modified: 2016-10-24T08:12:51Z
mutable provenance: E. coli, methanoba trcol OB3b
owned by: james
owned by: myers
mutable description: pCopA TAT Csp1 sfGFP with plasmid constitutively expressed CueR
discontinued: false
dominant: false
experience: experience/Issues
group u list: _2447_
m user id: 29607
owner id: 29607
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: aahahahah
star: false
creator: Sam Garforth
type: DnaRegion
role: partType/Other
role: SO:0000110
component: BBa_K1980006
component: BBa_K1980001
sequence annotation: BBa_K1980006
sequence annotation: BBa_K1980001
sequence: https://synbiohub.org/public/igem/BBa_K1980010_sequence/1

BBa_K1980006
type: Component
persistent identity: component2493041
display id: component2493041
version: 1
top level: BBa_K1980010
access: public
definition: BBa_K1980006

BBa_K1980001
type: Component
persistent identity: component2493042
display id: component2493042
version: 1
top level: BBa_K1980010
access: public
definition: Csp1sfGFP

BBa_K1980006
type: SequenceAnnotation
persistent identity: annotation2493041
display id: annotation2493041
version: 1
top level: BBa_K1980010
location: https://synbiohub.org/public/igem/BBa_K1980010/annotation2493041/range2493041/1
component: BBa_K1980006

https://synbiohub.org/public/igem/BBa_K1980010/annotation2493041/range2493041/1
type: Range
persistent identity: range2493041
display id: range2493041
version: 1
top level: BBa_K1980010
direction: direction/reverse
start: 1
end: 574
orientation: inline

BBa_K1980001
type: SequenceAnnotation
persistent identity: annotation2493042
display id: annotation2493042
version: 1
top level: BBa_K1980010
location: https://synbiohub.org/public/igem/BBa_K1980010/annotation2493042/range2493042/1
component: BBa_K1980001

https://synbiohub.org/public/igem/BBa_K1980010/annotation2493042/range2493042/1
type: Range
persistent identity: range2493042
display id: range2493042
version: 1
top level: BBa_K1980010
direction: direction/reverse
start: 581
end: 1783
orientation: inline

BBa_K1321151
type: ComponentDefinition
persistent identity: BBa K1321151
display id: BBa_K1321151
version: 1
was generated by: iGEM to SBOL conversion
description: metallothionein Fmt fused to CBDclos
created: 2014-10-08T11:00:00Z
modified: 2015-06-17T12:23:26Z
mutable provenance: GATC
owned by: james
owned by: myers
mutable description: GATC
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20961
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: GATC
star: false
creator: Gabriella Santosa
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321012
component: BBa_B0105
component: BBa_K1321002
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321012
sequence annotation: BBa_K1321002
sequence: https://synbiohub.org/public/igem/BBa_K1321151_sequence/1

BBa_K1321012
type: Component
persistent identity: component2415027
display id: component2415027
version: 1
top level: BBa_K1321151
access: public
definition: BBa_K1321012

BBa_B0105
type: Component
persistent identity: component2415029
display id: component2415029
version: 1
top level: BBa_K1321151
access: public
definition: Scar 25

BBa_K1321002
type: Component
persistent identity: component2415030
display id: component2415030
version: 1
top level: BBa_K1321151
access: public
definition: BBa_K1321002

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2415029
display id: annotation2415029
version: 1
top level: BBa_K1321151
location: https://synbiohub.org/public/igem/BBa_K1321151/annotation2415029/range2415029/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321151/annotation2415029/range2415029/1
type: Range
persistent identity: range2415029
display id: range2415029
version: 1
top level: BBa_K1321151
direction: direction/reverse
start: 199
end: 204
orientation: inline

BBa_K1321012
type: SequenceAnnotation
persistent identity: annotation2415027
display id: annotation2415027
version: 1
top level: BBa_K1321151
location: https://synbiohub.org/public/igem/BBa_K1321151/annotation2415027/range2415027/1
component: BBa_K1321012

https://synbiohub.org/public/igem/BBa_K1321151/annotation2415027/range2415027/1
type: Range
persistent identity: range2415027
display id: range2415027
version: 1
top level: BBa_K1321151
direction: direction/reverse
start: 1
end: 198
orientation: inline

BBa_K1321002
type: SequenceAnnotation
persistent identity: annotation2415030
display id: annotation2415030
version: 1
top level: BBa_K1321151
location: https://synbiohub.org/public/igem/BBa_K1321151/annotation2415030/range2415030/1
component: BBa_K1321002

https://synbiohub.org/public/igem/BBa_K1321151/annotation2415030/range2415030/1
type: Range
persistent identity: range2415030
display id: range2415030
version: 1
top level: BBa_K1321151
direction: direction/reverse
start: 205
end: 504
orientation: inline

BBa_K1321339
type: ComponentDefinition
persistent identity: BBa K1321339
display id: BBa_K1321339
version: 1
was generated by: iGEM to SBOL conversion
description: CBDcenA+Linker, RFC 25 standard
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:14:18Z
mutable provenance: asdf
owned by: james
owned by: myers
mutable description: CBDcenA in Freiburg format (RFC 25).
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: asdf
star: false
creator: Chris N Micklem
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: CBDCenA
sequence annotation: Linker: Pro-Thr box
sequence: https://synbiohub.org/public/igem/BBa_K1321339_sequence/1

CBDCenA
type: SequenceAnnotation
persistent identity: annotation2418065
display id: annotation2418065
version: 1
top level: BBa_K1321339
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1321339/annotation2418065/range2418065/1

https://synbiohub.org/public/igem/BBa_K1321339/annotation2418065/range2418065/1
type: Range
persistent identity: range2418065
display id: range2418065
version: 1
top level: BBa_K1321339
direction: direction/forward
start: 1
end: 318
orientation: inline

Linker: Pro-Thr box
type: SequenceAnnotation
persistent identity: annotation2414614
display id: annotation2414614
version: 1
top level: BBa_K1321339
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1321339/annotation2414614/range2414614/1

https://synbiohub.org/public/igem/BBa_K1321339/annotation2414614/range2414614/1
type: Range
persistent identity: range2414614
display id: range2414614
version: 1
top level: BBa_K1321339
direction: direction/forward
start: 319
end: 408
orientation: inline

BBa_K1420002
type: ComponentDefinition
persistent identity: BBa K1420002
display id: BBa_K1420002
version: 1
was generated by: iGEM to SBOL conversion
description: merB, Organomercurial Lyase from Serratia marcescens
created: 2014-10-08T11:00:00Z
modified: 2015-07-22T02:52:43Z
mutable provenance: Amplified from pDU1358, kindly donated by Anne O. Summers.
owned by: james
owned by: myers
mutable description: Demethylates organomerury.
discontinued: false
dominant: false
experience: experience/None
group u list: _1798_
m user id: 4206
owner id: 17872
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: false
creator: Stephen C. Heinsch
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: merB
sequence: https://synbiohub.org/public/igem/BBa_K1420002_sequence/1

merB
type: SequenceAnnotation
persistent identity: annotation2407616
display id: annotation2407616
version: 1
top level: BBa_K1420002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1420002/annotation2407616/range2407616/1

https://synbiohub.org/public/igem/BBa_K1420002/annotation2407616/range2407616/1
type: Range
persistent identity: range2407616
display id: range2407616
version: 1
top level: BBa_K1420002
direction: direction/forward
start: 1
end: 639
orientation: inline

BBa_K1933101
type: ComponentDefinition
persistent identity: BBa K1933101
display id: BBa_K1933101
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive expression of CBDclos fused to INPNC with 6xHis tag
created: 2016-10-09T11:00:00Z
modified: 2016-10-18T11:01:45Z
mutable provenance: Promoter and RBS: iGEM16_Kyoto The INPNC surface expression component derives from Pseudomonas Syringae. We used parts BBa_K811005 from iGEM12_Penn. CBDclos derives from Clostridium cellulovorans, and is part of a multi-unit cellulosomes. We used parts BBa_K1321341 from iGEM Imperial 2014 for construction.
owned by: james
owned by: myers
mutable description: CBDclos fused to INPNC with 6xHis tag is one of a series of surface expressing fusion proteins that make up biodevice that aims to be therapeutic solution against norovirus infections. This protein in particular is a cellulose binding protein(CBDclos) fused to surface expression anchoring domain(INPNC), connected by a 6xHis tag to be easily identified by Western blotting. For more information, please visit our Wiki page.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2400_
m user id: 23701
owner id: 29998
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: The stop codon for this part is not in the coding region, but in the suffix region.
star: false
creator: Tomoki Uchino
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1321002
component: BBa_K1933011
component: BBa_K1933004
sequence annotation: BBa_K1933004
sequence annotation: BBa_K1321002
sequence annotation: BBa_K1933011
sequence: https://synbiohub.org/public/igem/BBa_K1933101_sequence/1

BBa_K1321002
type: Component
persistent identity: component2515619
display id: component2515619
version: 1
top level: BBa_K1933101
access: public
definition: BBa_K1321002

BBa_K1933011
type: Component
persistent identity: component2515618
display id: component2515618
version: 1
top level: BBa_K1933101
access: public
definition: BBa_K1933011

BBa_K1933004
type: Component
persistent identity: component2515614
display id: component2515614
version: 1
top level: BBa_K1933101
access: public
definition: BBa_K1933004

BBa_K1933004
type: SequenceAnnotation
persistent identity: annotation2515614
display id: annotation2515614
version: 1
top level: BBa_K1933101
location: https://synbiohub.org/public/igem/BBa_K1933101/annotation2515614/range2515614/1
component: BBa_K1933004

https://synbiohub.org/public/igem/BBa_K1933101/annotation2515614/range2515614/1
type: Range
persistent identity: range2515614
display id: range2515614
version: 1
top level: BBa_K1933101
direction: direction/reverse
start: 1
end: 60
orientation: inline

BBa_K1321002
type: SequenceAnnotation
persistent identity: annotation2515619
display id: annotation2515619
version: 1
top level: BBa_K1933101
location: https://synbiohub.org/public/igem/BBa_K1933101/annotation2515619/range2515619/1
component: BBa_K1321002

https://synbiohub.org/public/igem/BBa_K1933101/annotation2515619/range2515619/1
type: Range
persistent identity: range2515619
display id: range2515619
version: 1
top level: BBa_K1933101
direction: direction/reverse
start: 1036
end: 1335
orientation: inline

BBa_K1933011
type: SequenceAnnotation
persistent identity: annotation2515618
display id: annotation2515618
version: 1
top level: BBa_K1933101
location: https://synbiohub.org/public/igem/BBa_K1933101/annotation2515618/range2515618/1
component: BBa_K1933011

https://synbiohub.org/public/igem/BBa_K1933101/annotation2515618/range2515618/1
type: Range
persistent identity: range2515618
display id: range2515618
version: 1
top level: BBa_K1933101
direction: direction/reverse
start: 61
end: 1035
orientation: inline

BBa_K1321121
type: ComponentDefinition
persistent identity: BBa K1321121
display id: BBa_K1321121
version: 1
was generated by: iGEM to SBOL conversion
description: Smt metallothionein fused to CBDclos
created: 2014-10-08T11:00:00Z
modified: 2015-06-17T12:22:33Z
mutable provenance: GATC
owned by: james
owned by: myers
mutable description: GATC
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20961
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: GATC
star: false
creator: Gabriella Santosa
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1321011
component: BBa_K1321002
component: BBa_B0105
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321011
sequence annotation: BBa_K1321002
sequence: https://synbiohub.org/public/igem/BBa_K1321121_sequence/1

BBa_K1321011
type: Component
persistent identity: component2414703
display id: component2414703
version: 1
top level: BBa_K1321121
access: public
definition: BBa_K1321011

BBa_K1321002
type: Component
persistent identity: component2414706
display id: component2414706
version: 1
top level: BBa_K1321121
access: public
definition: BBa_K1321002

BBa_B0105
type: Component
persistent identity: component2414705
display id: component2414705
version: 1
top level: BBa_K1321121
access: public
definition: Scar 25

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2414705
display id: annotation2414705
version: 1
top level: BBa_K1321121
location: https://synbiohub.org/public/igem/BBa_K1321121/annotation2414705/range2414705/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321121/annotation2414705/range2414705/1
type: Range
persistent identity: range2414705
display id: range2414705
version: 1
top level: BBa_K1321121
direction: direction/reverse
start: 166
end: 171
orientation: inline

BBa_K1321011
type: SequenceAnnotation
persistent identity: annotation2414703
display id: annotation2414703
version: 1
top level: BBa_K1321121
location: https://synbiohub.org/public/igem/BBa_K1321121/annotation2414703/range2414703/1
component: BBa_K1321011

https://synbiohub.org/public/igem/BBa_K1321121/annotation2414703/range2414703/1
type: Range
persistent identity: range2414703
display id: range2414703
version: 1
top level: BBa_K1321121
direction: direction/reverse
start: 1
end: 165
orientation: inline

BBa_K1321002
type: SequenceAnnotation
persistent identity: annotation2414706
display id: annotation2414706
version: 1
top level: BBa_K1321121
location: https://synbiohub.org/public/igem/BBa_K1321121/annotation2414706/range2414706/1
component: BBa_K1321002

https://synbiohub.org/public/igem/BBa_K1321121/annotation2414706/range2414706/1
type: Range
persistent identity: range2414706
display id: range2414706
version: 1
top level: BBa_K1321121
direction: direction/reverse
start: 172
end: 471
orientation: inline

BBa_K1321337
type: ComponentDefinition
persistent identity: BBa K1321337
display id: BBa_K1321337
version: 1
was generated by: iGEM to SBOL conversion
description: sfGFP in Freiburg format (RFC 25)
created: 2014-10-07T11:00:00Z
modified: 2016-01-26T02:10:16Z
mutable provenance: Made from existing part.
owned by: james
owned by: myers
mutable description: sfGFP in Freiburg format (RFC 25) to allow for easy use in fusion proteins.
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: OEPCR
star: false
creator: Chris N Micklem
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: sfGFP
sequence: https://synbiohub.org/public/igem/BBa_K1321337_sequence/1

sfGFP
type: SequenceAnnotation
persistent identity: annotation2417826
display id: annotation2417826
version: 1
top level: BBa_K1321337
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1321337/annotation2417826/range2417826/1

https://synbiohub.org/public/igem/BBa_K1321337/annotation2417826/range2417826/1
type: Range
persistent identity: range2417826
display id: range2417826
version: 1
top level: BBa_K1321337
direction: direction/forward
start: 1
end: 711
orientation: inline

BBa_K1404002
type: ComponentDefinition
persistent identity: BBa K1404002
display id: BBa_K1404002
version: 1
was generated by: iGEM to SBOL conversion
description: Ptac-NiCoTB, improves nickel and cobalt internalization
created: 2014-09-22T11:00:00Z
modified: 2015-07-23T03:01:16Z
mutable provenance: The promoter and the rest...
owned by: james
owned by: myers
mutable description: This part comprises a strong E. coli promoter with...
discontinued: false
dominant: false
experience: experience/Works
group u list: _1782_
m user id: 4206
owner id: 8419
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Temp
star: false
creator: Alexandre Duprey
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: Ptac
sequence annotation: RBS Paris 2009
sequence annotation: B0014 terminator
sequence annotation: NiCoT
sequence: https://synbiohub.org/public/igem/BBa_K1404002_sequence/1

Ptac
type: SequenceAnnotation
persistent identity: annotation2385405
display id: annotation2385405
version: 1
top level: BBa_K1404002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1404002/annotation2385405/range2385405/1

https://synbiohub.org/public/igem/BBa_K1404002/annotation2385405/range2385405/1
type: Range
persistent identity: range2385405
display id: range2385405
version: 1
top level: BBa_K1404002
direction: direction/forward
start: 1
end: 76
orientation: inline

RBS Paris 2009
type: SequenceAnnotation
persistent identity: annotation2385407
display id: annotation2385407
version: 1
top level: BBa_K1404002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1404002/annotation2385407/range2385407/1

https://synbiohub.org/public/igem/BBa_K1404002/annotation2385407/range2385407/1
type: Range
persistent identity: range2385407
display id: range2385407
version: 1
top level: BBa_K1404002
direction: direction/forward
start: 83
end: 95
orientation: inline

B0014 terminator
type: SequenceAnnotation
persistent identity: annotation2385408
display id: annotation2385408
version: 1
top level: BBa_K1404002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404002/annotation2385408/range2385408/1

https://synbiohub.org/public/igem/BBa_K1404002/annotation2385408/range2385408/1
type: Range
persistent identity: range2385408
display id: range2385408
version: 1
top level: BBa_K1404002
direction: direction/forward
start: 1212
end: 1306
orientation: inline

NiCoT
type: SequenceAnnotation
persistent identity: annotation2385406
display id: annotation2385406
version: 1
top level: BBa_K1404002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1404002/annotation2385406/range2385406/1

https://synbiohub.org/public/igem/BBa_K1404002/annotation2385406/range2385406/1
type: Range
persistent identity: range2385406
display id: range2385406
version: 1
top level: BBa_K1404002
direction: direction/forward
start: 96
end: 1212
orientation: inline

BBa_K1420003
type: ComponentDefinition
persistent identity: BBa K1420003
display id: BBa_K1420003
version: 1
was generated by: iGEM to SBOL conversion
description: MerP, mercuric transport protein periplasmic component
created: 2014-10-08T11:00:00Z
modified: 2015-07-22T02:53:24Z
mutable provenance: Source organism: Serratia marcescens. Plasmid pDU1358 kindly donated by Anne O. Summers.
owned by: james
owned by: myers
mutable description: merP is in charge of periplasmic transportation of mercury. This protein has two conserved cysteine residues for specific binding with the mercury ion, Hg(II).
discontinued: false
dominant: false
experience: experience/None
group u list: _1798_
m user id: 4206
owner id: 17872
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: false
creator: Stephen C. Heinsch
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: merP
sequence: https://synbiohub.org/public/igem/BBa_K1420003_sequence/1

merP
type: SequenceAnnotation
persistent identity: annotation2407638
display id: annotation2407638
version: 1
top level: BBa_K1420003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1420003/annotation2407638/range2407638/1

https://synbiohub.org/public/igem/BBa_K1420003/annotation2407638/range2407638/1
type: Range
persistent identity: range2407638
display id: range2407638
version: 1
top level: BBa_K1420003
direction: direction/forward
start: 1
end: 276
orientation: inline

BBa_K863113
type: ComponentDefinition
persistent identity: BBa K863113
display id: BBa_K863113
version: 1
was generated by: iGEM to SBOL conversion
description: Cellulose binding Domain of C. cellulovorans cellulose binding protein with Reporter GFP
created: 2012-09-19T11:00:00Z
modified: 2015-06-17T01:20:29Z
mutable provenance: Cloning-vector and [http://partsregistry.org/Part:BBa_K863121 BBa_K863121]
owned by: james
owned by: myers
mutable description: Cellulose binding Domain from Clostridium cellulovorans [http://www.ncbi.nlm.nih.gov/nuccore/M73817 cellulose binding protein gene] with T7, RBS linked with two amino acids (GS) to a GFP with His-tag.
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 4206
owner id: 13080
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: CBDclos fused to a his-tagged GFP in a Freiburg-manner
star: false
creator: Moritz M??ller
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: RBS
sequence annotation: Cellulose Binding Protein
sequence annotation: BBa_K525998
sequence annotation: ATG
sequence annotation: BBa_K863111
sequence annotation: Freiburg-Scar
sequence annotation: AgeI
sequence annotation: B0034
sequence annotation: BBa_K863112
sequence annotation: BBa_E0040
sequence annotation: TAA
sequence annotation: T7 promoter
sequence annotation: Silent mutation (A original)
sequence annotation: GS-Linker
sequence annotation: Cellulose binding domain (CBDclos)
sequence annotation: BBa_K863121
sequence annotation: His-Tag
sequence: https://synbiohub.org/public/igem/BBa_K863113_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2188390
display id: annotation2188390
version: 1
top level: BBa_K863113
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188390/range2188390/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188390/range2188390/1
type: Range
persistent identity: range2188390
display id: range2188390
version: 1
top level: BBa_K863113
direction: direction/forward
start: 24
end: 27
orientation: inline

Cellulose Binding Protein
type: SequenceAnnotation
persistent identity: annotation2188395
display id: annotation2188395
version: 1
top level: BBa_K863113
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188395/range2188395/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188395/range2188395/1
type: Range
persistent identity: range2188395
display id: range2188395
version: 1
top level: BBa_K863113
direction: direction/forward
start: 317
end: 322
orientation: inline

BBa_K525998
type: SequenceAnnotation
persistent identity: annotation2188387
display id: annotation2188387
version: 1
top level: BBa_K863113
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188387/range2188387/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188387/range2188387/1
type: Range
persistent identity: range2188387
display id: range2188387
version: 1
top level: BBa_K863113
direction: direction/forward
start: 1
end: 32
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2188391
display id: annotation2188391
version: 1
top level: BBa_K863113
role: feature/start
role: SO:0000318
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188391/range2188391/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188391/range2188391/1
type: Range
persistent identity: range2188391
display id: range2188391
version: 1
top level: BBa_K863113
direction: direction/forward
start: 35
end: 38
orientation: inline

BBa_K863111
type: SequenceAnnotation
persistent identity: annotation2188393
display id: annotation2188393
version: 1
top level: BBa_K863113
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188393/range2188393/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188393/range2188393/1
type: Range
persistent identity: range2188393
display id: range2188393
version: 1
top level: BBa_K863113
direction: direction/forward
start: 35
end: 328
orientation: inline

Freiburg-Scar
type: SequenceAnnotation
persistent identity: annotation2188397
display id: annotation2188397
version: 1
top level: BBa_K863113
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188397/range2188397/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188397/range2188397/1
type: Range
persistent identity: range2188397
display id: range2188397
version: 1
top level: BBa_K863113
direction: direction/forward
start: 329
end: 334
orientation: inline

Cellulose Binding Protein
type: SequenceAnnotation
persistent identity: annotation2188392
display id: annotation2188392
version: 1
top level: BBa_K863113
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188392/range2188392/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188392/range2188392/1
type: Range
persistent identity: range2188392
display id: range2188392
version: 1
top level: BBa_K863113
direction: direction/forward
start: 35
end: 40
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2188401
display id: annotation2188401
version: 1
top level: BBa_K863113
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188401/range2188401/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188401/range2188401/1
type: Range
persistent identity: range2188401
display id: range2188401
version: 1
top level: BBa_K863113
direction: direction/forward
start: 1068
end: 1072
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2188389
display id: annotation2188389
version: 1
top level: BBa_K863113
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188389/range2188389/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188389/range2188389/1
type: Range
persistent identity: range2188389
display id: range2188389
version: 1
top level: BBa_K863113
direction: direction/forward
start: 21
end: 32
orientation: inline

BBa_K863112
type: SequenceAnnotation
persistent identity: annotation2188386
display id: annotation2188386
version: 1
top level: BBa_K863113
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188386/range2188386/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188386/range2188386/1
type: Range
persistent identity: range2188386
display id: range2188386
version: 1
top level: BBa_K863113
direction: direction/reverse
start: 1
end: 328
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2188399
display id: annotation2188399
version: 1
top level: BBa_K863113
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188399/range2188399/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188399/range2188399/1
type: Range
persistent identity: range2188399
display id: range2188399
version: 1
top level: BBa_K863113
direction: direction/forward
start: 335
end: 1048
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2188402
display id: annotation2188402
version: 1
top level: BBa_K863113
role: feature/stop
role: SO:0000319
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188402/range2188402/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188402/range2188402/1
type: Range
persistent identity: range2188402
display id: range2188402
version: 1
top level: BBa_K863113
direction: direction/forward
start: 1073
end: 1075
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2188388
display id: annotation2188388
version: 1
top level: BBa_K863113
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188388/range2188388/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188388/range2188388/1
type: Range
persistent identity: range2188388
display id: range2188388
version: 1
top level: BBa_K863113
direction: direction/forward
start: 1
end: 20
orientation: inline

Silent mutation (A original)
type: SequenceAnnotation
persistent identity: annotation2200501
display id: annotation2200501
version: 1
top level: BBa_K863113
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2200501/range2200501/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2200501/range2200501/1
type: Range
persistent identity: range2200501
display id: range2200501
version: 1
top level: BBa_K863113
direction: direction/forward
start: 112
end: 112
orientation: inline

GS-Linker
type: SequenceAnnotation
persistent identity: annotation2188396
display id: annotation2188396
version: 1
top level: BBa_K863113
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188396/range2188396/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188396/range2188396/1
type: Range
persistent identity: range2188396
display id: range2188396
version: 1
top level: BBa_K863113
direction: direction/forward
start: 323
end: 328
orientation: inline

Cellulose binding domain (CBDclos)
type: SequenceAnnotation
persistent identity: annotation2188394
display id: annotation2188394
version: 1
top level: BBa_K863113
role: feature/protein
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188394/range2188394/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188394/range2188394/1
type: Range
persistent identity: range2188394
display id: range2188394
version: 1
top level: BBa_K863113
direction: direction/forward
start: 41
end: 316
orientation: inline

BBa_K863121
type: SequenceAnnotation
persistent identity: annotation2188398
display id: annotation2188398
version: 1
top level: BBa_K863113
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188398/range2188398/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188398/range2188398/1
type: Range
persistent identity: range2188398
display id: range2188398
version: 1
top level: BBa_K863113
direction: direction/reverse
start: 335
end: 1075
orientation: inline

His-Tag
type: SequenceAnnotation
persistent identity: annotation2188400
display id: annotation2188400
version: 1
top level: BBa_K863113
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188400/range2188400/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188400/range2188400/1
type: Range
persistent identity: range2188400
display id: range2188400
version: 1
top level: BBa_K863113
direction: direction/forward
start: 1049
end: 1066
orientation: inline

MaTif
type: ComponentDefinition
persistent identity: BBa K2127007
display id: BBa_K2127007
version: 1
was generated by: iGEM to SBOL conversion
description: MT - TERMINATOR
created: 2016-10-13T11:00:00Z
modified: 2016-10-21T05:23:57Z
mutable provenance: MT - TERMINATOR
owned by: james
owned by: myers
mutable description: MT - TERMINATOR
discontinued: false
dominant: false
experience: experience/None
group u list: _2596_
m user id: 32415
owner id: 32736
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: MT - TERMINATOR
star: false
creator: Mirat Sojitra
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_K2127007_sequence/1

BBa_K1321340
type: ComponentDefinition
persistent identity: BBa K1321340
display id: BBa_K1321340
version: 1
was generated by: iGEM to SBOL conversion
description: Double CBD (dCBD) with N-terminal linker
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:14:46Z
mutable provenance: asdf
owned by: james
owned by: myers
mutable description: dCBD with N-terminal linker.
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: asdf
star: false
creator: Chris N Micklem
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: dCBD
sequence annotation: Linker
sequence annotation: cbh1 CBD
sequence annotation: cbh2 CBD
sequence: https://synbiohub.org/public/igem/BBa_K1321340_sequence/1

dCBD
type: SequenceAnnotation
persistent identity: annotation2417847
display id: annotation2417847
version: 1
top level: BBa_K1321340
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1321340/annotation2417847/range2417847/1

https://synbiohub.org/public/igem/BBa_K1321340/annotation2417847/range2417847/1
type: Range
persistent identity: range2417847
display id: range2417847
version: 1
top level: BBa_K1321340
direction: direction/forward
start: 1
end: 366
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2417848
display id: annotation2417848
version: 1
top level: BBa_K1321340
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1321340/annotation2417848/range2417848/1

https://synbiohub.org/public/igem/BBa_K1321340/annotation2417848/range2417848/1
type: Range
persistent identity: range2417848
display id: range2417848
version: 1
top level: BBa_K1321340
direction: direction/forward
start: 1
end: 72
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2417850
display id: annotation2417850
version: 1
top level: BBa_K1321340
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1321340/annotation2417850/range2417850/1

https://synbiohub.org/public/igem/BBa_K1321340/annotation2417850/range2417850/1
type: Range
persistent identity: range2417850
display id: range2417850
version: 1
top level: BBa_K1321340
direction: direction/forward
start: 187
end: 258
orientation: inline

cbh1 CBD
type: SequenceAnnotation
persistent identity: annotation2417851
display id: annotation2417851
version: 1
top level: BBa_K1321340
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1321340/annotation2417851/range2417851/1

https://synbiohub.org/public/igem/BBa_K1321340/annotation2417851/range2417851/1
type: Range
persistent identity: range2417851
display id: range2417851
version: 1
top level: BBa_K1321340
direction: direction/forward
start: 259
end: 366
orientation: inline

cbh2 CBD
type: SequenceAnnotation
persistent identity: annotation2417849
display id: annotation2417849
version: 1
top level: BBa_K1321340
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1321340/annotation2417849/range2417849/1

https://synbiohub.org/public/igem/BBa_K1321340/annotation2417849/range2417849/1
type: Range
persistent identity: range2417849
display id: range2417849
version: 1
top level: BBa_K1321340
direction: direction/forward
start: 73
end: 186
orientation: inline

BBa_K1460006
type: ComponentDefinition
persistent identity: BBa K1460006
display id: BBa_K1460006
version: 1
was generated by: iGEM to SBOL conversion
description: Anderson Promoter + nixA + Ter + pT7 + GST-CRS5 + Ter
created: 2014-08-25T11:00:00Z
modified: 2016-01-13T10:18:58Z
mutable provenance: This part is a composite of BBa_K1460003 and BBa_K1460001.
owned by: james
owned by: myers
mutable description: This composite part functionally codes for the selective nickel transporter nixA as well as the metallothioniein CRS5 fused to glutathione-S-transferase. This composite part should act to transport nickel ions into the engineered cell where they will be sequestered by the metallothionein allowing for Ni2+ removal from contaminated water.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1839_
m user id: 4206
owner id: 15649
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: Expression of GST-CRS5 is controlled by the bacteriophage T7 promoter, so part should be combined with a host organism expressing T7 polymerase (such as E. coli BL21-AI
star: false
creator: Eric Holmes
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1460003
component: BBa_K1460001
sequence annotation: BBa_K1460003
sequence annotation: BBa_K1460001
sequence: https://synbiohub.org/public/igem/BBa_K1460006_sequence/1

BBa_K1460003
type: Component
persistent identity: component2381558
display id: component2381558
version: 1
top level: BBa_K1460006
access: public
definition: BBa_K1460003

BBa_K1460001
type: Component
persistent identity: component2381565
display id: component2381565
version: 1
top level: BBa_K1460006
access: public
definition: BBa_K1460001

BBa_K1460003
type: SequenceAnnotation
persistent identity: annotation2381558
display id: annotation2381558
version: 1
top level: BBa_K1460006
location: https://synbiohub.org/public/igem/BBa_K1460006/annotation2381558/range2381558/1
component: BBa_K1460003

https://synbiohub.org/public/igem/BBa_K1460006/annotation2381558/range2381558/1
type: Range
persistent identity: range2381558
display id: range2381558
version: 1
top level: BBa_K1460006
direction: direction/reverse
start: 1
end: 1029
orientation: inline

BBa_K1460001
type: SequenceAnnotation
persistent identity: annotation2381565
display id: annotation2381565
version: 1
top level: BBa_K1460006
location: https://synbiohub.org/public/igem/BBa_K1460006/annotation2381565/range2381565/1
component: BBa_K1460001

https://synbiohub.org/public/igem/BBa_K1460006/annotation2381565/range2381565/1
type: Range
persistent identity: range2381565
display id: range2381565
version: 1
top level: BBa_K1460006
direction: direction/reverse
start: 1038
end: 2065
orientation: inline

Csp1
type: ComponentDefinition
persistent identity: BBa K1980000
display id: BBa_K1980000
version: 1
was generated by: iGEM to SBOL conversion
description: TAT Copper Storage Protein 1
created: 2016-10-10T11:00:00Z
modified: 2016-10-12T02:23:43Z
mutable provenance: The source organism for the main protein sequence is Methylosinus trichosporium OB3b. The TAT sequence originate from E. coli multi copper oxidase enzyme CueO. We ordered it as codon optimised DNA from IDT.
owned by: james
owned by: myers
mutable description: Copper Storage protein 1 (Csp1) is a tetrameric copper storage protein found in the periplasm of Methylosinus trichosporium OB3b. We investigated whether this part could act as a copper chelator when expressed in E. coli. We modified the protein by adding a TAT signal peptide from the E. coli enzyme CueO in place of the native TAT sequence and a C terminal his tag in an attempt to direct the protein to the periplasm.
discontinued: false
dominant: false
experience: experience/Issues
group u list: _2447_
m user id: 29607
owner id: 29607
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Codon optimised for E. coli. TAT sequence changed to an E. coli TAT sequence.
star: false
creator: Sam Garforth
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: Hexa Histidine
sequence annotation: Csp1
sequence annotation: TAT signal peptide
sequence: https://synbiohub.org/public/igem/BBa_K1980000_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2491869
display id: annotation2491869
version: 1
top level: Csp1
role: feature/stop
role: SO:0000319
location: https://synbiohub.org/public/igem/BBa_K1980000/annotation2491869/range2491869/1

https://synbiohub.org/public/igem/BBa_K1980000/annotation2491869/range2491869/1
type: Range
persistent identity: range2491869
display id: range2491869
version: 1
top level: Csp1
direction: direction/forward
start: 469
end: 474
orientation: inline

Hexa Histidine
type: SequenceAnnotation
persistent identity: annotation2491868
display id: annotation2491868
version: 1
top level: Csp1
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1980000/annotation2491868/range2491868/1

https://synbiohub.org/public/igem/BBa_K1980000/annotation2491868/range2491868/1
type: Range
persistent identity: range2491868
display id: range2491868
version: 1
top level: Csp1
direction: direction/forward
start: 450
end: 468
orientation: inline

Csp1
type: SequenceAnnotation
persistent identity: annotation2491867
display id: annotation2491867
version: 1
top level: Csp1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1980000/annotation2491867/range2491867/1

https://synbiohub.org/public/igem/BBa_K1980000/annotation2491867/range2491867/1
type: Range
persistent identity: range2491867
display id: range2491867
version: 1
top level: Csp1
direction: direction/forward
start: 85
end: 450
orientation: inline

TAT signal peptide
type: SequenceAnnotation
persistent identity: annotation2491866
display id: annotation2491866
version: 1
top level: Csp1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1980000/annotation2491866/range2491866/1

https://synbiohub.org/public/igem/BBa_K1980000/annotation2491866/range2491866/1
type: Range
persistent identity: range2491866
display id: range2491866
version: 1
top level: Csp1
direction: direction/forward
start: 1
end: 84
orientation: inline

BBa_K1321154
type: ComponentDefinition
persistent identity: BBa K1321154
display id: BBa_K1321154
version: 1
was generated by: iGEM to SBOL conversion
description: CBDclos fused to metallothionein Fmt
created: 2014-10-08T11:00:00Z
modified: 2015-06-17T12:23:47Z
mutable provenance: GATC
owned by: james
owned by: myers
mutable description: GATC
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20961
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: GATC
star: false
creator: Gabriella Santosa
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321002
component: BBa_B0105
component: BBa_K1321012
sequence annotation: BBa_K1321012
sequence annotation: BBa_K1321002
sequence annotation: BBa_B0105
sequence: https://synbiohub.org/public/igem/BBa_K1321154_sequence/1

BBa_K1321002
type: Component
persistent identity: component2415053
display id: component2415053
version: 1
top level: BBa_K1321154
access: public
definition: BBa_K1321002

BBa_B0105
type: Component
persistent identity: component2415055
display id: component2415055
version: 1
top level: BBa_K1321154
access: public
definition: Scar 25

BBa_K1321012
type: Component
persistent identity: component2415057
display id: component2415057
version: 1
top level: BBa_K1321154
access: public
definition: BBa_K1321012

BBa_K1321012
type: SequenceAnnotation
persistent identity: annotation2415057
display id: annotation2415057
version: 1
top level: BBa_K1321154
location: https://synbiohub.org/public/igem/BBa_K1321154/annotation2415057/range2415057/1
component: BBa_K1321012

https://synbiohub.org/public/igem/BBa_K1321154/annotation2415057/range2415057/1
type: Range
persistent identity: range2415057
display id: range2415057
version: 1
top level: BBa_K1321154
direction: direction/reverse
start: 307
end: 504
orientation: inline

BBa_K1321002
type: SequenceAnnotation
persistent identity: annotation2415053
display id: annotation2415053
version: 1
top level: BBa_K1321154
location: https://synbiohub.org/public/igem/BBa_K1321154/annotation2415053/range2415053/1
component: BBa_K1321002

https://synbiohub.org/public/igem/BBa_K1321154/annotation2415053/range2415053/1
type: Range
persistent identity: range2415053
display id: range2415053
version: 1
top level: BBa_K1321154
direction: direction/reverse
start: 1
end: 300
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2415055
display id: annotation2415055
version: 1
top level: BBa_K1321154
location: https://synbiohub.org/public/igem/BBa_K1321154/annotation2415055/range2415055/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321154/annotation2415055/range2415055/1
type: Range
persistent identity: range2415055
display id: range2415055
version: 1
top level: BBa_K1321154
direction: direction/reverse
start: 301
end: 306
orientation: inline

BBa_K1933102
type: ComponentDefinition
persistent identity: BBa K1933102
display id: BBa_K1933102
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive expression of CBDcex fused to BclA with 6xHis tag
created: 2016-10-09T11:00:00Z
modified: 2016-10-10T12:04:53Z
mutable provenance: Promoter and RBS:BBa_K1933004 The BclA surface expression component derives from Bacillus anthracis(34F2), it is a hair-like protein that covers B. anthracis??? spores. We used parts BBa_K1933000 from iGEMKyoto 2016 for construction. We outsourced chemical synthesis of this part for construction. CBDcex derives from Cellulomonas fimi, and is part of an exoglucanase secreted by this organism. We used parts BBa_K1321342 from iGEM Imperial 2014 for construction.
owned by: james
owned by: myers
mutable description: CBDcex fused to BclA with 6xHis tag is one of a series of surface expressing fusion proteins that make up biodevice that aims to be therapeutic solution against norovirus infections. This protein in particular is a cellulose binding protein(CBD) fused to surface expression anchoring domain(BclA), connected by a 6xHis tag to be easily identified by Westernblotting. For more information, please visit our Wiki page.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2400_
m user id: 23701
owner id: 29998
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: The stop codon for this part is not in the coding region, but in the suffix region.
star: false
creator: Tomoki Uchino
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1321003
component: BBa_K1933004
component: BBa_K1933000
sequence annotation: BBa_K1933004
sequence annotation: BBa_K1321003
sequence annotation: BBa_K1933000
sequence: https://synbiohub.org/public/igem/BBa_K1933102_sequence/1

BBa_K1321003
type: Component
persistent identity: component2491092
display id: component2491092
version: 1
top level: BBa_K1933102
access: public
definition: BBa_K1321003

BBa_K1933004
type: Component
persistent identity: component2491087
display id: component2491087
version: 1
top level: BBa_K1933102
access: public
definition: BBa_K1933004

BBa_K1933000
type: Component
persistent identity: component2491091
display id: component2491091
version: 1
top level: BBa_K1933102
access: public
definition: BclA-His

BBa_K1933004
type: SequenceAnnotation
persistent identity: annotation2491087
display id: annotation2491087
version: 1
top level: BBa_K1933102
location: https://synbiohub.org/public/igem/BBa_K1933102/annotation2491087/range2491087/1
component: BBa_K1933004

https://synbiohub.org/public/igem/BBa_K1933102/annotation2491087/range2491087/1
type: Range
persistent identity: range2491087
display id: range2491087
version: 1
top level: BBa_K1933102
direction: direction/reverse
start: 1
end: 60
orientation: inline

BBa_K1321003
type: SequenceAnnotation
persistent identity: annotation2491092
display id: annotation2491092
version: 1
top level: BBa_K1933102
location: https://synbiohub.org/public/igem/BBa_K1933102/annotation2491092/range2491092/1
component: BBa_K1321003

https://synbiohub.org/public/igem/BBa_K1933102/annotation2491092/range2491092/1
type: Range
persistent identity: range2491092
display id: range2491092
version: 1
top level: BBa_K1933102
direction: direction/reverse
start: 145
end: 471
orientation: inline

BBa_K1933000
type: SequenceAnnotation
persistent identity: annotation2491091
display id: annotation2491091
version: 1
top level: BBa_K1933102
location: https://synbiohub.org/public/igem/BBa_K1933102/annotation2491091/range2491091/1
component: BBa_K1933000

https://synbiohub.org/public/igem/BBa_K1933102/annotation2491091/range2491091/1
type: Range
persistent identity: range2491091
display id: range2491091
version: 1
top level: BBa_K1933102
direction: direction/reverse
start: 61
end: 144
orientation: inline

BBa_K1321097
type: ComponentDefinition
persistent identity: BBa K1321097
display id: BBa_K1321097
version: 1
was generated by: iGEM to SBOL conversion
description: CBDcenA-linker fused to Phytochelatin (PC) EC20
created: 2014-10-06T11:00:00Z
modified: 2015-06-17T12:34:51Z
mutable provenance: CBD15+PhytoC. TBC
owned by: james
owned by: myers
mutable description: CBD15+PhytoC. TBC
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20780
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: CBD15+PhytoC. TBC
star: false
creator: Xenia Spencer-Milnes
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321340
component: BBa_K1321005
component: BBa_B0105
sequence annotation: BBa_K1321005
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321340
sequence: https://synbiohub.org/public/igem/BBa_K1321097_sequence/1

BBa_K1321340
type: Component
persistent identity: component2414740
display id: component2414740
version: 1
top level: BBa_K1321097
access: public
definition: BBa_K1321340

BBa_K1321005
type: Component
persistent identity: component2414744
display id: component2414744
version: 1
top level: BBa_K1321097
access: public
definition: BBa_K1321005

BBa_B0105
type: Component
persistent identity: component2414742
display id: component2414742
version: 1
top level: BBa_K1321097
access: public
definition: Scar 25

BBa_K1321005
type: SequenceAnnotation
persistent identity: annotation2414744
display id: annotation2414744
version: 1
top level: BBa_K1321097
location: https://synbiohub.org/public/igem/BBa_K1321097/annotation2414744/range2414744/1
component: BBa_K1321005

https://synbiohub.org/public/igem/BBa_K1321097/annotation2414744/range2414744/1
type: Range
persistent identity: range2414744
display id: range2414744
version: 1
top level: BBa_K1321097
direction: direction/reverse
start: 389
end: 511
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2414742
display id: annotation2414742
version: 1
top level: BBa_K1321097
location: https://synbiohub.org/public/igem/BBa_K1321097/annotation2414742/range2414742/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321097/annotation2414742/range2414742/1
type: Range
persistent identity: range2414742
display id: range2414742
version: 1
top level: BBa_K1321097
direction: direction/reverse
start: 375
end: 380
orientation: inline

BBa_K1321340
type: SequenceAnnotation
persistent identity: annotation2414740
display id: annotation2414740
version: 1
top level: BBa_K1321097
location: https://synbiohub.org/public/igem/BBa_K1321097/annotation2414740/range2414740/1
component: BBa_K1321340

https://synbiohub.org/public/igem/BBa_K1321097/annotation2414740/range2414740/1
type: Range
persistent identity: range2414740
display id: range2414740
version: 1
top level: BBa_K1321097
direction: direction/reverse
start: 1
end: 366
orientation: inline

BBa_K863110
type: ComponentDefinition
persistent identity: BBa K863110
display id: BBa_K863110
version: 1
was generated by: iGEM to SBOL conversion
description: Cellulose binding Domain from Clostridium cellulovorans cellulose binding protein
created: 2012-09-19T11:00:00Z
modified: 2015-06-17T01:20:08Z
mutable provenance: Cloning-vector
owned by: james
owned by: myers
mutable description: Cellulose binding Domain from Clostridium cellulovorans [http://www.ncbi.nlm.nih.gov/nuccore/M73817 cellulose binding protein gene] in Freiburg-Standard.
discontinued: false
dominant: false
group u list: _1123_
m user id: 4206
owner id: 13080
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Added Freiburg-pre- and suffix for in frame assembly and a two amino acids (GS)-Linker.
star: false
creator: Moritz M??ller
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: CBP
sequence annotation: NgoMIV
sequence annotation: Cellulose binding domain (CBDclos)
sequence annotation: TAA
sequence annotation: AgeI
sequence annotation: ATG
sequence: https://synbiohub.org/public/igem/BBa_K863110_sequence/1

CBP
type: SequenceAnnotation
persistent identity: annotation2188407
display id: annotation2188407
version: 1
top level: BBa_K863110
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863110/annotation2188407/range2188407/1

https://synbiohub.org/public/igem/BBa_K863110/annotation2188407/range2188407/1
type: Range
persistent identity: range2188407
display id: range2188407
version: 1
top level: BBa_K863110
direction: direction/forward
start: 298
end: 303
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2188265
display id: annotation2188265
version: 1
top level: BBa_K863110
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863110/annotation2188265/range2188265/1

https://synbiohub.org/public/igem/BBa_K863110/annotation2188265/range2188265/1
type: Range
persistent identity: range2188265
display id: range2188265
version: 1
top level: BBa_K863110
direction: direction/forward
start: 4
end: 9
orientation: inline

Cellulose binding domain (CBDclos)
type: SequenceAnnotation
persistent identity: annotation2188266
display id: annotation2188266
version: 1
top level: BBa_K863110
role: feature/protein
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863110/annotation2188266/range2188266/1

https://synbiohub.org/public/igem/BBa_K863110/annotation2188266/range2188266/1
type: Range
persistent identity: range2188266
display id: range2188266
version: 1
top level: BBa_K863110
direction: direction/forward
start: 22
end: 297
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2188268
display id: annotation2188268
version: 1
top level: BBa_K863110
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863110/annotation2188268/range2188268/1

https://synbiohub.org/public/igem/BBa_K863110/annotation2188268/range2188268/1
type: Range
persistent identity: range2188268
display id: range2188268
version: 1
top level: BBa_K863110
direction: direction/forward
start: 310
end: 312
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2188267
display id: annotation2188267
version: 1
top level: BBa_K863110
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863110/annotation2188267/range2188267/1

https://synbiohub.org/public/igem/BBa_K863110/annotation2188267/range2188267/1
type: Range
persistent identity: range2188267
display id: range2188267
version: 1
top level: BBa_K863110
direction: direction/forward
start: 304
end: 309
orientation: inline

CBP
type: SequenceAnnotation
persistent identity: annotation2188406
display id: annotation2188406
version: 1
top level: BBa_K863110
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863110/annotation2188406/range2188406/1

https://synbiohub.org/public/igem/BBa_K863110/annotation2188406/range2188406/1
type: Range
persistent identity: range2188406
display id: range2188406
version: 1
top level: BBa_K863110
direction: direction/forward
start: 10
end: 21
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2188264
display id: annotation2188264
version: 1
top level: BBa_K863110
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863110/annotation2188264/range2188264/1

https://synbiohub.org/public/igem/BBa_K863110/annotation2188264/range2188264/1
type: Range
persistent identity: range2188264
display id: range2188264
version: 1
top level: BBa_K863110
direction: direction/forward
start: 1
end: 3
orientation: inline

BBa_K1355000
type: ComponentDefinition
persistent identity: BBa K1355000
display id: BBa_K1355000
version: 1
was generated by: iGEM to SBOL conversion
description: Strong RBS + merA (mercuric ion reductase)+ terminator (BBa_ B0015)
created: 2014-10-05T11:00:00Z
modified: 2016-01-13T10:34:49Z
mutable provenance: MerA gene sequence is found in the O26-CRL plasmid from Escherichia coli O26. We also added strong RBS from protein 10 found in phage 17 and the double terminator (BBa_B0015) to ensure efficient termination of transcription and messenger RNA recognition.
owned by: james
owned by: myers
mutable description: The MerA gene is one of the most important gene into bacterial mercury-resistance operon. Transforms mercury Hg2+ in Hg0, which is volatile and moves passively out of the bacteria in gaseous form as shown in Figure above. The reduction of Hg2+ occurs by a NADPH-dependent system.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1731_
m user id: 4206
owner id: 15499
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: We analyzed all Mercury-resistance (Mer) operon genes, in these following strains available at NCBI: (1) Escherichia coli 042, (2) Escherichia coli O26 , (3) Escherichia coli ACN001, (4) Escherichia coli 838C-R1, (5) Escherichia coli APEC-O103, (6) Escherichia coli UMNK88, (7) Escherichia coli AR060302, (8) Escherichia coli PMV-1. It is showed that the mer genes from strains (1) - (2) - (3) - (4) - (5) is conserved among them. However strains (6) - (7) and (8) did not show any similarity. Therefore we used sequences from (1) to (5) due the consensus in E.coli.
star: false
creator: Luna Barroco de Lacerda
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: MerA
sequence annotation: rbs
sequence annotation: BBa_ B0015
sequence: https://synbiohub.org/public/igem/BBa_K1355000_sequence/1

MerA
type: SequenceAnnotation
persistent identity: annotation2396893
display id: annotation2396893
version: 1
top level: BBa_K1355000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1355000/annotation2396893/range2396893/1

https://synbiohub.org/public/igem/BBa_K1355000/annotation2396893/range2396893/1
type: Range
persistent identity: range2396893
display id: range2396893
version: 1
top level: BBa_K1355000
direction: direction/forward
start: 24
end: 1639
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2396890
display id: annotation2396890
version: 1
top level: BBa_K1355000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1355000/annotation2396890/range2396890/1

https://synbiohub.org/public/igem/BBa_K1355000/annotation2396890/range2396890/1
type: Range
persistent identity: range2396890
display id: range2396890
version: 1
top level: BBa_K1355000
direction: direction/forward
start: 9
end: 16
orientation: inline

BBa_ B0015
type: SequenceAnnotation
persistent identity: annotation2396891
display id: annotation2396891
version: 1
top level: BBa_K1355000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1355000/annotation2396891/range2396891/1

https://synbiohub.org/public/igem/BBa_K1355000/annotation2396891/range2396891/1
type: Range
persistent identity: range2396891
display id: range2396891
version: 1
top level: BBa_K1355000
direction: direction/forward
start: 1652
end: 1778
orientation: inline

BBa_K1321342
type: ComponentDefinition
persistent identity: BBa K1321342
display id: BBa_K1321342
version: 1
was generated by: iGEM to SBOL conversion
description: sfGFP fused to CBDcex in RFC 25
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:26:42Z
mutable provenance: asdf
owned by: james
owned by: myers
mutable description: sadf
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: asdf
star: false
creator: Chris N Micklem
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321003
component: BBa_K1321337
component: BBa_B0105
sequence annotation: BBa_K1321337
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321003
sequence: https://synbiohub.org/public/igem/BBa_K1321342_sequence/1

BBa_K1321003
type: Component
persistent identity: component2415362
display id: component2415362
version: 1
top level: BBa_K1321342
access: public
definition: BBa_K1321003

BBa_K1321337
type: Component
persistent identity: component2415359
display id: component2415359
version: 1
top level: BBa_K1321342
access: public
definition: BBa_K1321337

BBa_B0105
type: Component
persistent identity: component2415361
display id: component2415361
version: 1
top level: BBa_K1321342
access: public
definition: Scar 25

BBa_K1321337
type: SequenceAnnotation
persistent identity: annotation2415359
display id: annotation2415359
version: 1
top level: BBa_K1321342
location: https://synbiohub.org/public/igem/BBa_K1321342/annotation2415359/range2415359/1
component: BBa_K1321337

https://synbiohub.org/public/igem/BBa_K1321342/annotation2415359/range2415359/1
type: Range
persistent identity: range2415359
display id: range2415359
version: 1
top level: BBa_K1321342
direction: direction/reverse
start: 1
end: 711
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2415361
display id: annotation2415361
version: 1
top level: BBa_K1321342
location: https://synbiohub.org/public/igem/BBa_K1321342/annotation2415361/range2415361/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321342/annotation2415361/range2415361/1
type: Range
persistent identity: range2415361
display id: range2415361
version: 1
top level: BBa_K1321342
direction: direction/reverse
start: 712
end: 717
orientation: inline

BBa_K1321003
type: SequenceAnnotation
persistent identity: annotation2415362
display id: annotation2415362
version: 1
top level: BBa_K1321342
location: https://synbiohub.org/public/igem/BBa_K1321342/annotation2415362/range2415362/1
component: BBa_K1321003

https://synbiohub.org/public/igem/BBa_K1321342/annotation2415362/range2415362/1
type: Range
persistent identity: range2415362
display id: range2415362
version: 1
top level: BBa_K1321342
direction: direction/reverse
start: 718
end: 1044
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
access: public
definition: BBa_B0012

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
access: public
definition: BBa_B0010

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

CBDcex(T7)
type: ComponentDefinition
persistent identity: BBa K863102
display id: BBa_K863102
version: 1
was generated by: iGEM to SBOL conversion
description: Cellulose binding Domain of C. Fimi Exoglucanase with T7, RBS, GS-Linker (Freiburg-Standard)
created: 2012-09-18T11:00:00Z
modified: 2015-06-17T01:19:09Z
mutable provenance: Cloning-vector
owned by: james
owned by: myers
mutable description: Cellulose binding domain of the [http://www.ncbi.nlm.nih.gov/nucleotide/327179207?report=genbank&log$=nucltop&blast_rank=3&RID=152ZCN0E01N Cellulomonas fimi ATCC 484 exoglucanase gene] in Freiburg-standard with T7, RBS, GS-Linker (Freiburg-Standard)
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 4206
owner id: 13080
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: You can find additional info to the Cellulose binding domain here: [http://partsregistry.org/Part:BBa_K863101:Design CBDcex_Freiburg]
star: false
creator: Moritz M??ller
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: B0034
sequence annotation: Exoglucanase
sequence annotation: TAA
sequence annotation: T7 promoter
sequence annotation: GS-Linker
sequence annotation: RBS
sequence annotation: BBa_K863101
sequence annotation: ATG
sequence annotation: Cellulose binding domain (CBDcex)
sequence annotation: AgeI
sequence annotation: BBa_K525998
sequence: https://synbiohub.org/public/igem/BBa_K863102_sequence/1

B0034
type: SequenceAnnotation
persistent identity: annotation2187505
display id: annotation2187505
version: 1
top level: CBDcex(T7)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187505/range2187505/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187505/range2187505/1
type: Range
persistent identity: range2187505
display id: range2187505
version: 1
top level: CBDcex(T7)
direction: direction/forward
start: 21
end: 32
orientation: inline

Exoglucanase
type: SequenceAnnotation
persistent identity: annotation2187508
display id: annotation2187508
version: 1
top level: CBDcex(T7)
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187508/range2187508/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187508/range2187508/1
type: Range
persistent identity: range2187508
display id: range2187508
version: 1
top level: CBDcex(T7)
direction: direction/forward
start: 38
end: 49
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2187514
display id: annotation2187514
version: 1
top level: CBDcex(T7)
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187514/range2187514/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187514/range2187514/1
type: Range
persistent identity: range2187514
display id: range2187514
version: 1
top level: CBDcex(T7)
direction: direction/forward
start: 371
end: 373
orientation: inline

Exoglucanase
type: SequenceAnnotation
persistent identity: annotation2187511
display id: annotation2187511
version: 1
top level: CBDcex(T7)
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187511/range2187511/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187511/range2187511/1
type: Range
persistent identity: range2187511
display id: range2187511
version: 1
top level: CBDcex(T7)
direction: direction/forward
start: 350
end: 358
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2187503
display id: annotation2187503
version: 1
top level: CBDcex(T7)
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187503/range2187503/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187503/range2187503/1
type: Range
persistent identity: range2187503
display id: range2187503
version: 1
top level: CBDcex(T7)
direction: direction/forward
start: 1
end: 20
orientation: inline

GS-Linker
type: SequenceAnnotation
persistent identity: annotation2187512
display id: annotation2187512
version: 1
top level: CBDcex(T7)
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187512/range2187512/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187512/range2187512/1
type: Range
persistent identity: range2187512
display id: range2187512
version: 1
top level: CBDcex(T7)
direction: direction/forward
start: 359
end: 364
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2187506
display id: annotation2187506
version: 1
top level: CBDcex(T7)
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187506/range2187506/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187506/range2187506/1
type: Range
persistent identity: range2187506
display id: range2187506
version: 1
top level: CBDcex(T7)
direction: direction/forward
start: 24
end: 27
orientation: inline

BBa_K863101
type: SequenceAnnotation
persistent identity: annotation2187509
display id: annotation2187509
version: 1
top level: CBDcex(T7)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187509/range2187509/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187509/range2187509/1
type: Range
persistent identity: range2187509
display id: range2187509
version: 1
top level: CBDcex(T7)
direction: direction/forward
start: 38
end: 373
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2187507
display id: annotation2187507
version: 1
top level: CBDcex(T7)
role: feature/start
role: SO:0000318
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187507/range2187507/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187507/range2187507/1
type: Range
persistent identity: range2187507
display id: range2187507
version: 1
top level: CBDcex(T7)
direction: direction/forward
start: 35
end: 37
orientation: inline

Cellulose binding domain (CBDcex)
type: SequenceAnnotation
persistent identity: annotation2187510
display id: annotation2187510
version: 1
top level: CBDcex(T7)
role: feature/protein
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187510/range2187510/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187510/range2187510/1
type: Range
persistent identity: range2187510
display id: range2187510
version: 1
top level: CBDcex(T7)
direction: direction/forward
start: 50
end: 349
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2187513
display id: annotation2187513
version: 1
top level: CBDcex(T7)
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187513/range2187513/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187513/range2187513/1
type: Range
persistent identity: range2187513
display id: range2187513
version: 1
top level: CBDcex(T7)
direction: direction/forward
start: 365
end: 370
orientation: inline

BBa_K525998
type: SequenceAnnotation
persistent identity: annotation2187504
display id: annotation2187504
version: 1
top level: CBDcex(T7)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187504/range2187504/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187504/range2187504/1
type: Range
persistent identity: range2187504
display id: range2187504
version: 1
top level: CBDcex(T7)
direction: direction/forward
start: 1
end: 32
orientation: inline

BBa_K1505002
type: ComponentDefinition
persistent identity: BBa K1505002
display id: BBa_K1505002
version: 1
was generated by: iGEM to SBOL conversion
description: RBS+mntH+mCerulean
created: 2014-10-09T11:00:00Z
modified: 2016-01-13T10:36:51Z
mutable provenance: RBS is predicted from pmsp3 promoter from Magnetospirillum magneticum strain AMB-1 genome. MntH is from part BBa_K1505000. mCerulean is from part BBa_K133066.
owned by: james
owned by: myers
mutable description: The RBS is from pmsp3 promoter, the strongest promoter isolated from Magnetospirillum bacteria. mntH was functionally expressed in Azospirillum sp. B510. It encodes a divalent metal cation transporter. The MntH protein and mCerulean protein are linked by a fusion protein linker. The purpose of the linkage is to improve the expression/folding of MntH and for better visualization of successful transformation. The original use is to express MntH in Magnetospirillum magneticum strain AMB-1.
discontinued: false
dominant: false
experience: experience/None
group u list: _1885_
m user id: 4206
owner id: 22465
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: We researched the optimum linker sequence for fusion protein to facilitate successful fusion. The mCerulean protein is expected to improve the folding of MntH in AMB-1.
star: false
creator: Jane Shmushkis, Amey Vrudhula
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: mCerulean
sequence annotation: rbs (from pmsp3)
sequence annotation: mntH
sequence annotation: ggggs linker
sequence: https://synbiohub.org/public/igem/BBa_K1505002_sequence/1

mCerulean
type: SequenceAnnotation
persistent identity: annotation2412330
display id: annotation2412330
version: 1
top level: BBa_K1505002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1505002/annotation2412330/range2412330/1

https://synbiohub.org/public/igem/BBa_K1505002/annotation2412330/range2412330/1
type: Range
persistent identity: range2412330
display id: range2412330
version: 1
top level: BBa_K1505002
direction: direction/forward
start: 1318
end: 2034
orientation: inline

rbs (from pmsp3)
type: SequenceAnnotation
persistent identity: annotation2412327
display id: annotation2412327
version: 1
top level: BBa_K1505002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1505002/annotation2412327/range2412327/1

https://synbiohub.org/public/igem/BBa_K1505002/annotation2412327/range2412327/1
type: Range
persistent identity: range2412327
display id: range2412327
version: 1
top level: BBa_K1505002
direction: direction/forward
start: 19
end: 46
orientation: inline

mntH
type: SequenceAnnotation
persistent identity: annotation2412328
display id: annotation2412328
version: 1
top level: BBa_K1505002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1505002/annotation2412328/range2412328/1

https://synbiohub.org/public/igem/BBa_K1505002/annotation2412328/range2412328/1
type: Range
persistent identity: range2412328
display id: range2412328
version: 1
top level: BBa_K1505002
direction: direction/forward
start: 67
end: 1302
orientation: inline

ggggs linker
type: SequenceAnnotation
persistent identity: annotation2412329
display id: annotation2412329
version: 1
top level: BBa_K1505002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1505002/annotation2412329/range2412329/1

https://synbiohub.org/public/igem/BBa_K1505002/annotation2412329/range2412329/1
type: Range
persistent identity: range2412329
display id: range2412329
version: 1
top level: BBa_K1505002
direction: direction/forward
start: 1303
end: 1317
orientation: inline

BBa_K190020
type: ComponentDefinition
persistent identity: BBa K190020
display id: BBa_K190020
version: 1
was generated by: iGEM to SBOL conversion
description: MymT
created: 2009-08-18T11:00:00Z
modified: 2016-01-13T12:55:12Z
mutable provenance: MymT was cloned from pGB68 (kindly provided by Ben Gold, Cornell University), the gene's origin is the genome of ''M. tuberculosis''
owned by: james
owned by: myers
mutable description: MymT is a methallothionein binding Cu (II)
discontinued: false
dominant: false
experience: experience/None
group u list: _306_
m user id: 4206
owner id: 4147
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: BioBrick prefix and suffix was added by PCR, also the RBS (B0034) was added by PCR (not in the sequence yet).
star: false
creator: Nienke Kuipers
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: MymT
sequence annotation: B0034
sequence: https://synbiohub.org/public/igem/BBa_K190020_sequence/1

MymT
type: SequenceAnnotation
persistent identity: annotation2017541
display id: annotation2017541
version: 1
top level: BBa_K190020
role: feature/protein
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K190020/annotation2017541/range2017541/1

https://synbiohub.org/public/igem/BBa_K190020/annotation2017541/range2017541/1
type: Range
persistent identity: range2017541
display id: range2017541
version: 1
top level: BBa_K190020
direction: direction/forward
start: 19
end: 180
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2017540
display id: annotation2017540
version: 1
top level: BBa_K190020
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K190020/annotation2017540/range2017540/1

https://synbiohub.org/public/igem/BBa_K190020/annotation2017540/range2017540/1
type: Range
persistent identity: range2017540
display id: range2017540
version: 1
top level: BBa_K190020
direction: direction/forward
start: 1
end: 18
orientation: inline

BBa_K1321011
type: ComponentDefinition
persistent identity: BBa K1321011
display id: BBa_K1321011
version: 1
was generated by: iGEM to SBOL conversion
description: SmtA metallothionein in Freiburg format (RFC[25])
created: 2014-10-09T11:00:00Z
modified: 2015-05-08T01:09:51Z
mutable provenance: Source from BBa_____
owned by: james
owned by: myers
mutable description: SmtA metallothionein in rfc25 format. This part is based on BBa_____ but has been codon optimised for E.coli and is in the rfc25 biobrick format for ease of use as part of protein fusions. This part is available as fusions as follows:
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 0
owner id: 20780
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Codon optimised for E.coli. rbs not included in the part as it is for fusions
star: false
creator: Xenia Spencer-Milnes
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: SmtA
sequence: https://synbiohub.org/public/igem/BBa_K1321011_sequence/1

SmtA
type: SequenceAnnotation
persistent identity: annotation2414600
display id: annotation2414600
version: 1
top level: BBa_K1321011
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1321011/annotation2414600/range2414600/1

https://synbiohub.org/public/igem/BBa_K1321011/annotation2414600/range2414600/1
type: Range
persistent identity: range2414600
display id: range2414600
version: 1
top level: BBa_K1321011
direction: direction/forward
start: 1
end: 165
orientation: inline

BBa_K1980006
type: ComponentDefinition
persistent identity: BBa K1980006
display id: BBa_K1980006
version: 1
was generated by: iGEM to SBOL conversion
description: pCopA with divergent expressed CueR
created: 2016-10-10T11:00:00Z
modified: 2016-10-23T04:58:33Z
mutable provenance: E. coli
owned by: james
owned by: myers
mutable description: pCopA with plasmid constitutively expressed CueR (no reporter)
discontinued: false
dominant: false
experience: experience/Works
group u list: _2447_
m user id: 29607
owner id: 29607
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Codon optimised. Designed for biobrick assembly with reporter of choice
star: false
creator: Sam Garforth
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: RBS
sequence annotation: Double stop
sequence annotation: Constitutive promoter
sequence annotation: pCopA
sequence annotation: CueR
sequence: https://synbiohub.org/public/igem/BBa_K1980006_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2495263
display id: annotation2495263
version: 1
top level: BBa_K1980006
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1980006/annotation2495263/range2495263/1

https://synbiohub.org/public/igem/BBa_K1980006/annotation2495263/range2495263/1
type: Range
persistent identity: range2495263
display id: range2495263
version: 1
top level: BBa_K1980006
direction: direction/reverse
start: 418
end: 429
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2495260
display id: annotation2495260
version: 1
top level: BBa_K1980006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1980006/annotation2495260/range2495260/1

https://synbiohub.org/public/igem/BBa_K1980006/annotation2495260/range2495260/1
type: Range
persistent identity: range2495260
display id: range2495260
version: 1
top level: BBa_K1980006
direction: direction/forward
start: 563
end: 574
orientation: inline

Double stop
type: SequenceAnnotation
persistent identity: annotation2495261
display id: annotation2495261
version: 1
top level: BBa_K1980006
role: feature/stop
role: SO:0000319
location: https://synbiohub.org/public/igem/BBa_K1980006/annotation2495261/range2495261/1

https://synbiohub.org/public/igem/BBa_K1980006/annotation2495261/range2495261/1
type: Range
persistent identity: range2495261
display id: range2495261
version: 1
top level: BBa_K1980006
direction: direction/reverse
start: 1
end: 7
orientation: inline

Constitutive promoter
type: SequenceAnnotation
persistent identity: annotation2495264
display id: annotation2495264
version: 1
top level: BBa_K1980006
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1980006/annotation2495264/range2495264/1

https://synbiohub.org/public/igem/BBa_K1980006/annotation2495264/range2495264/1
type: Range
persistent identity: range2495264
display id: range2495264
version: 1
top level: BBa_K1980006
direction: direction/reverse
start: 439
end: 472
orientation: inline

pCopA
type: SequenceAnnotation
persistent identity: annotation2495265
display id: annotation2495265
version: 1
top level: BBa_K1980006
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1980006/annotation2495265/range2495265/1

https://synbiohub.org/public/igem/BBa_K1980006/annotation2495265/range2495265/1
type: Range
persistent identity: range2495265
display id: range2495265
version: 1
top level: BBa_K1980006
direction: direction/forward
start: 506
end: 556
orientation: inline

CueR
type: SequenceAnnotation
persistent identity: annotation2495262
display id: annotation2495262
version: 1
top level: BBa_K1980006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1980006/annotation2495262/range2495262/1

https://synbiohub.org/public/igem/BBa_K1980006/annotation2495262/range2495262/1
type: Range
persistent identity: range2495262
display id: range2495262
version: 1
top level: BBa_K1980006
direction: direction/reverse
start: 7
end: 411
orientation: inline

CBDclos(J6
type: ComponentDefinition
persistent identity: BBa K863114
display id: BBa_K863114
version: 1
was generated by: iGEM to SBOL conversion
description: CBD of C. cellulovorans cellulose binding protein gene with const. Promoter (J23100+J61101) and GFP
created: 2012-09-22T11:00:00Z
modified: 2015-06-17T01:20:44Z
mutable provenance: Cellulose binding domain form a cloning-vector. J61101 and [http://partsregistry.org/Part:BBa_I13522 GFP] from the partsregistry.
owned by: james
owned by: myers
mutable description: Cellulose binding Domain of Clostridium cellulovorans [http://www.ncbi.nlm.nih.gov/nuccore/M73817 cellulose binding protein gene (cbp A)] with a constitutive promoter and RBS ( J61101 ) and a fused-GFP protein with His-tag.
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 4206
owner id: 13080
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Was made to switch from a T7-promoter to a constitutive promoter
star: false
creator: Moritz M??ller
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: BBa_K863121
sequence annotation: GS-Linker
sequence annotation: BBa_K863111
sequence annotation: BBa_J61101
sequence annotation: Cellulose Binding Protein
sequence annotation: BBa_E0040
sequence annotation: Freiburg-Scar
sequence annotation: TAA
sequence annotation: AgeI
sequence annotation: ATG
sequence annotation: BBa_J23100
sequence annotation: Cellulose binding domain (CBDclos)
sequence annotation: His-Tag
sequence annotation: NgoMIV
sequence: https://synbiohub.org/public/igem/BBa_K863114_sequence/1

BBa_K863121
type: SequenceAnnotation
persistent identity: annotation2194139
display id: annotation2194139
version: 1
top level: CBDclos(J6
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194139/range2194139/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194139/range2194139/1
type: Range
persistent identity: range2194139
display id: range2194139
version: 1
top level: CBDclos(J6
direction: direction/reverse
start: 377
end: 1117
orientation: inline

GS-Linker
type: SequenceAnnotation
persistent identity: annotation2194137
display id: annotation2194137
version: 1
top level: CBDclos(J6
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194137/range2194137/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194137/range2194137/1
type: Range
persistent identity: range2194137
display id: range2194137
version: 1
top level: CBDclos(J6
direction: direction/forward
start: 365
end: 370
orientation: inline

BBa_K863111
type: SequenceAnnotation
persistent identity: annotation2194131
display id: annotation2194131
version: 1
top level: CBDclos(J6
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194131/range2194131/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194131/range2194131/1
type: Range
persistent identity: range2194131
display id: range2194131
version: 1
top level: CBDclos(J6
direction: direction/reverse
start: 62
end: 370
orientation: inline

BBa_J61101
type: SequenceAnnotation
persistent identity: annotation2194130
display id: annotation2194130
version: 1
top level: CBDclos(J6
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194130/range2194130/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194130/range2194130/1
type: Range
persistent identity: range2194130
display id: range2194130
version: 1
top level: CBDclos(J6
direction: direction/reverse
start: 44
end: 55
orientation: inline

Cellulose Binding Protein
type: SequenceAnnotation
persistent identity: annotation2194134
display id: annotation2194134
version: 1
top level: CBDclos(J6
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194134/range2194134/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194134/range2194134/1
type: Range
persistent identity: range2194134
display id: range2194134
version: 1
top level: CBDclos(J6
direction: direction/forward
start: 71
end: 82
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2194140
display id: annotation2194140
version: 1
top level: CBDclos(J6
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194140/range2194140/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194140/range2194140/1
type: Range
persistent identity: range2194140
display id: range2194140
version: 1
top level: CBDclos(J6
direction: direction/forward
start: 377
end: 1090
orientation: inline

Cellulose Binding Protein
type: SequenceAnnotation
persistent identity: annotation2194136
display id: annotation2194136
version: 1
top level: CBDclos(J6
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194136/range2194136/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194136/range2194136/1
type: Range
persistent identity: range2194136
display id: range2194136
version: 1
top level: CBDclos(J6
direction: direction/forward
start: 359
end: 364
orientation: inline

Freiburg-Scar
type: SequenceAnnotation
persistent identity: annotation2194138
display id: annotation2194138
version: 1
top level: CBDclos(J6
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194138/range2194138/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194138/range2194138/1
type: Range
persistent identity: range2194138
display id: range2194138
version: 1
top level: CBDclos(J6
direction: direction/forward
start: 371
end: 376
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2194143
display id: annotation2194143
version: 1
top level: CBDclos(J6
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194143/range2194143/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194143/range2194143/1
type: Range
persistent identity: range2194143
display id: range2194143
version: 1
top level: CBDclos(J6
direction: direction/forward
start: 1115
end: 1117
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2194142
display id: annotation2194142
version: 1
top level: CBDclos(J6
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194142/range2194142/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194142/range2194142/1
type: Range
persistent identity: range2194142
display id: range2194142
version: 1
top level: CBDclos(J6
direction: direction/forward
start: 1109
end: 1114
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2194132
display id: annotation2194132
version: 1
top level: CBDclos(J6
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194132/range2194132/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194132/range2194132/1
type: Range
persistent identity: range2194132
display id: range2194132
version: 1
top level: CBDclos(J6
direction: direction/forward
start: 62
end: 64
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2194129
display id: annotation2194129
version: 1
top level: CBDclos(J6
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194129/range2194129/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194129/range2194129/1
type: Range
persistent identity: range2194129
display id: range2194129
version: 1
top level: CBDclos(J6
direction: direction/reverse
start: 1
end: 35
orientation: inline

Cellulose binding domain (CBDclos)
type: SequenceAnnotation
persistent identity: annotation2194135
display id: annotation2194135
version: 1
top level: CBDclos(J6
role: feature/protein
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194135/range2194135/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194135/range2194135/1
type: Range
persistent identity: range2194135
display id: range2194135
version: 1
top level: CBDclos(J6
direction: direction/forward
start: 83
end: 358
orientation: inline

His-Tag
type: SequenceAnnotation
persistent identity: annotation2194141
display id: annotation2194141
version: 1
top level: CBDclos(J6
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194141/range2194141/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194141/range2194141/1
type: Range
persistent identity: range2194141
display id: range2194141
version: 1
top level: CBDclos(J6
direction: direction/forward
start: 1091
end: 1108
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2194133
display id: annotation2194133
version: 1
top level: CBDclos(J6
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194133/range2194133/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194133/range2194133/1
type: Range
persistent identity: range2194133
display id: range2194133
version: 1
top level: CBDclos(J6
direction: direction/forward
start: 65
end: 70
orientation: inline

BBa_K1321348
type: ComponentDefinition
persistent identity: BBa K1321348
display id: BBa_K1321348
version: 1
was generated by: iGEM to SBOL conversion
description: sfGFP fused to dCBD with linker in RFC 25
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:30:41Z
mutable provenance: asdf
owned by: james
owned by: myers
mutable description: asdf
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: asdf
star: false
creator: Chris N Micklem
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321337
component: BBa_B0105
component: BBa_K1321340
sequence annotation: BBa_K1321340
sequence annotation: BBa_K1321337
sequence annotation: BBa_B0105
sequence: https://synbiohub.org/public/igem/BBa_K1321348_sequence/1

BBa_K1321337
type: Component
persistent identity: component2415136
display id: component2415136
version: 1
top level: BBa_K1321348
access: public
definition: BBa_K1321337

BBa_B0105
type: Component
persistent identity: component2415138
display id: component2415138
version: 1
top level: BBa_K1321348
access: public
definition: Scar 25

BBa_K1321340
type: Component
persistent identity: component2415140
display id: component2415140
version: 1
top level: BBa_K1321348
access: public
definition: BBa_K1321340

BBa_K1321340
type: SequenceAnnotation
persistent identity: annotation2415140
display id: annotation2415140
version: 1
top level: BBa_K1321348
location: https://synbiohub.org/public/igem/BBa_K1321348/annotation2415140/range2415140/1
component: BBa_K1321340

https://synbiohub.org/public/igem/BBa_K1321348/annotation2415140/range2415140/1
type: Range
persistent identity: range2415140
display id: range2415140
version: 1
top level: BBa_K1321348
direction: direction/reverse
start: 718
end: 1083
orientation: inline

BBa_K1321337
type: SequenceAnnotation
persistent identity: annotation2415136
display id: annotation2415136
version: 1
top level: BBa_K1321348
location: https://synbiohub.org/public/igem/BBa_K1321348/annotation2415136/range2415136/1
component: BBa_K1321337

https://synbiohub.org/public/igem/BBa_K1321348/annotation2415136/range2415136/1
type: Range
persistent identity: range2415136
display id: range2415136
version: 1
top level: BBa_K1321348
direction: direction/reverse
start: 1
end: 711
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2415138
display id: annotation2415138
version: 1
top level: BBa_K1321348
location: https://synbiohub.org/public/igem/BBa_K1321348/annotation2415138/range2415138/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321348/annotation2415138/range2415138/1
type: Range
persistent identity: range2415138
display id: range2415138
version: 1
top level: BBa_K1321348
direction: direction/reverse
start: 712
end: 717
orientation: inline

BBa_K1460007
type: ComponentDefinition
persistent identity: BBa K1460007
display id: BBa_K1460007
version: 1
was generated by: iGEM to SBOL conversion
description: Anderson Promoter + merT + merP + Ter + pT7 + GST-CRS5 + Ter
created: 2014-08-25T11:00:00Z
modified: 2016-01-13T10:19:27Z
mutable provenance: This part is a composite of parts BBa_K1460004 and BBa_K1460001
owned by: james
owned by: myers
mutable description: This composite part functionally codes for the selective mercury transporters merT and merP as well as the metallothioniein CRS5 fused to glutathione-S-transferase. This composite part should act to transport mercury ions into the engineered cell where they will be sequestered by the metallothionein allowing for Hg2+ removal from contaminated water.
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1839_
m user id: 4206
owner id: 15649
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Expression of GST-CRS5 is controlled by the bacteriophage T7 promoter, so part should be combined with a host organism expressing T7 polymerase (such as E. coli BL21-AI from Life Technologies).
star: false
creator: Eric Holmes
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1460001
component: BBa_K1460004
sequence annotation: BBa_K1460001
sequence annotation: BBa_K1460004
sequence: https://synbiohub.org/public/igem/BBa_K1460007_sequence/1

BBa_K1460001
type: Component
persistent identity: component2381578
display id: component2381578
version: 1
top level: BBa_K1460007
access: public
definition: BBa_K1460001

BBa_K1460004
type: Component
persistent identity: component2381571
display id: component2381571
version: 1
top level: BBa_K1460007
access: public
definition: BBa_K1460004

BBa_K1460001
type: SequenceAnnotation
persistent identity: annotation2381578
display id: annotation2381578
version: 1
top level: BBa_K1460007
location: https://synbiohub.org/public/igem/BBa_K1460007/annotation2381578/range2381578/1
component: BBa_K1460001

https://synbiohub.org/public/igem/BBa_K1460007/annotation2381578/range2381578/1
type: Range
persistent identity: range2381578
display id: range2381578
version: 1
top level: BBa_K1460007
direction: direction/reverse
start: 750
end: 1777
orientation: inline

BBa_K1460004
type: SequenceAnnotation
persistent identity: annotation2381571
display id: annotation2381571
version: 1
top level: BBa_K1460007
location: https://synbiohub.org/public/igem/BBa_K1460007/annotation2381571/range2381571/1
component: BBa_K1460004

https://synbiohub.org/public/igem/BBa_K1460007/annotation2381571/range2381571/1
type: Range
persistent identity: range2381571
display id: range2381571
version: 1
top level: BBa_K1460007
direction: direction/reverse
start: 1
end: 741
orientation: inline

BBa_K1321341
type: ComponentDefinition
persistent identity: BBa K1321341
display id: BBa_K1321341
version: 1
was generated by: iGEM to SBOL conversion
description: sfGFP fused to CBDclos in RFC 25
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:27:02Z
mutable provenance: asdfad
owned by: james
owned by: myers
mutable description: asdfads
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: asdfd
star: false
creator: Chris N Micklem
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321337
component: BBa_K1321002
component: BBa_B0105
sequence annotation: BBa_K1321002
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321337
sequence: https://synbiohub.org/public/igem/BBa_K1321341_sequence/1

BBa_K1321337
type: Component
persistent identity: component2415088
display id: component2415088
version: 1
top level: BBa_K1321341
access: public
definition: BBa_K1321337

BBa_K1321002
type: Component
persistent identity: component2415091
display id: component2415091
version: 1
top level: BBa_K1321341
access: public
definition: BBa_K1321002

BBa_B0105
type: Component
persistent identity: component2415090
display id: component2415090
version: 1
top level: BBa_K1321341
access: public
definition: Scar 25

BBa_K1321002
type: SequenceAnnotation
persistent identity: annotation2415091
display id: annotation2415091
version: 1
top level: BBa_K1321341
location: https://synbiohub.org/public/igem/BBa_K1321341/annotation2415091/range2415091/1
component: BBa_K1321002

https://synbiohub.org/public/igem/BBa_K1321341/annotation2415091/range2415091/1
type: Range
persistent identity: range2415091
display id: range2415091
version: 1
top level: BBa_K1321341
direction: direction/reverse
start: 718
end: 1017
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2415090
display id: annotation2415090
version: 1
top level: BBa_K1321341
location: https://synbiohub.org/public/igem/BBa_K1321341/annotation2415090/range2415090/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321341/annotation2415090/range2415090/1
type: Range
persistent identity: range2415090
display id: range2415090
version: 1
top level: BBa_K1321341
direction: direction/reverse
start: 712
end: 717
orientation: inline

BBa_K1321337
type: SequenceAnnotation
persistent identity: annotation2415088
display id: annotation2415088
version: 1
top level: BBa_K1321341
location: https://synbiohub.org/public/igem/BBa_K1321341/annotation2415088/range2415088/1
component: BBa_K1321337

https://synbiohub.org/public/igem/BBa_K1321341/annotation2415088/range2415088/1
type: Range
persistent identity: range2415088
display id: range2415088
version: 1
top level: BBa_K1321341
direction: direction/reverse
start: 1
end: 711
orientation: inline

BBa_K1321112
type: ComponentDefinition
persistent identity: BBa K1321112
display id: BBa_K1321112
version: 1
was generated by: iGEM to SBOL conversion
description: Phytochelatin (PC) EC20 fused to CBDcex with LacI promoter
created: 2014-10-08T11:00:00Z
modified: 2015-06-17T12:19:44Z
mutable provenance: PhytoC+CBD7 with LacI
owned by: james
owned by: myers
mutable description: PhytoC+CBD7 with LacI
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20780
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: PhytoC+CBD7 with LacI
star: false
creator: Xenia Spencer-Milnes
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321005
component: BBa_J04500
component: BBa_K1321003
component: BBa_K1319106
component: BBa_B0105
sequence annotation: BBa_J04500
sequence annotation: BBa_K1321003
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321005
sequence annotation: BBa_K1319106
sequence: https://synbiohub.org/public/igem/BBa_K1321112_sequence/1

BBa_K1321005
type: Component
persistent identity: component2414915
display id: component2414915
version: 1
top level: BBa_K1321112
access: public
definition: BBa_K1321005

BBa_J04500
type: Component
persistent identity: component2414911
display id: component2414911
version: 1
top level: BBa_K1321112
access: public
definition: BBa_J04500

BBa_K1321003
type: Component
persistent identity: component2414918
display id: component2414918
version: 1
top level: BBa_K1321112
access: public
definition: BBa_K1321003

BBa_K1319106
type: Component
persistent identity: component2414913
display id: component2414913
version: 1
top level: BBa_K1321112
access: public
definition: Scar25 ATG

BBa_B0105
type: Component
persistent identity: component2414917
display id: component2414917
version: 1
top level: BBa_K1321112
access: public
definition: Scar 25

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2414911
display id: annotation2414911
version: 1
top level: BBa_K1321112
location: https://synbiohub.org/public/igem/BBa_K1321112/annotation2414911/range2414911/1
component: BBa_J04500

https://synbiohub.org/public/igem/BBa_K1321112/annotation2414911/range2414911/1
type: Range
persistent identity: range2414911
display id: range2414911
version: 1
top level: BBa_K1321112
direction: direction/reverse
start: 1
end: 220
orientation: inline

BBa_K1321003
type: SequenceAnnotation
persistent identity: annotation2414918
display id: annotation2414918
version: 1
top level: BBa_K1321112
location: https://synbiohub.org/public/igem/BBa_K1321112/annotation2414918/range2414918/1
component: BBa_K1321003

https://synbiohub.org/public/igem/BBa_K1321112/annotation2414918/range2414918/1
type: Range
persistent identity: range2414918
display id: range2414918
version: 1
top level: BBa_K1321112
direction: direction/reverse
start: 365
end: 691
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2414917
display id: annotation2414917
version: 1
top level: BBa_K1321112
location: https://synbiohub.org/public/igem/BBa_K1321112/annotation2414917/range2414917/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321112/annotation2414917/range2414917/1
type: Range
persistent identity: range2414917
display id: range2414917
version: 1
top level: BBa_K1321112
direction: direction/reverse
start: 359
end: 364
orientation: inline

BBa_K1321005
type: SequenceAnnotation
persistent identity: annotation2414915
display id: annotation2414915
version: 1
top level: BBa_K1321112
location: https://synbiohub.org/public/igem/BBa_K1321112/annotation2414915/range2414915/1
component: BBa_K1321005

https://synbiohub.org/public/igem/BBa_K1321112/annotation2414915/range2414915/1
type: Range
persistent identity: range2414915
display id: range2414915
version: 1
top level: BBa_K1321112
direction: direction/reverse
start: 236
end: 358
orientation: inline

BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2414913
display id: annotation2414913
version: 1
top level: BBa_K1321112
location: https://synbiohub.org/public/igem/BBa_K1321112/annotation2414913/range2414913/1
component: BBa_K1319106

https://synbiohub.org/public/igem/BBa_K1321112/annotation2414913/range2414913/1
type: Range
persistent identity: range2414913
display id: range2414913
version: 1
top level: BBa_K1321112
direction: direction/reverse
start: 221
end: 235
orientation: inline

BBa_K1321352
type: ComponentDefinition
persistent identity: BBa K1321352
display id: BBa_K1321352
version: 1
was generated by: iGEM to SBOL conversion
description: NiBP fused to CBDcex driven by T7
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:31:54Z
mutable provenance: asdf
owned by: james
owned by: myers
mutable description: asdf
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: asdf
star: false
creator: Chris N Micklem
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1319106
component: BBa_K1321344
component: BBa_K1321338
sequence annotation: BBa_K1321338
sequence annotation: BBa_K1319106
sequence annotation: BBa_K1321344
sequence: https://synbiohub.org/public/igem/BBa_K1321352_sequence/1

BBa_K1319106
type: Component
persistent identity: component2415430
display id: component2415430
version: 1
top level: BBa_K1321352
access: public
definition: Scar25 ATG

BBa_K1321344
type: Component
persistent identity: component2415435
display id: component2415435
version: 1
top level: BBa_K1321352
access: public
definition: BBa_K1321344

BBa_K1321338
type: Component
persistent identity: component2415428
display id: component2415428
version: 1
top level: BBa_K1321352
access: public
definition: BBa_K1321338

BBa_K1321338
type: SequenceAnnotation
persistent identity: annotation2415428
display id: annotation2415428
version: 1
top level: BBa_K1321352
location: https://synbiohub.org/public/igem/BBa_K1321352/annotation2415428/range2415428/1
component: BBa_K1321338

https://synbiohub.org/public/igem/BBa_K1321352/annotation2415428/range2415428/1
type: Range
persistent identity: range2415428
display id: range2415428
version: 1
top level: BBa_K1321352
direction: direction/reverse
start: 1
end: 43
orientation: inline

BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2415430
display id: annotation2415430
version: 1
top level: BBa_K1321352
location: https://synbiohub.org/public/igem/BBa_K1321352/annotation2415430/range2415430/1
component: BBa_K1319106

https://synbiohub.org/public/igem/BBa_K1321352/annotation2415430/range2415430/1
type: Range
persistent identity: range2415430
display id: range2415430
version: 1
top level: BBa_K1321352
direction: direction/reverse
start: 44
end: 58
orientation: inline

BBa_K1321344
type: SequenceAnnotation
persistent identity: annotation2415435
display id: annotation2415435
version: 1
top level: BBa_K1321352
location: https://synbiohub.org/public/igem/BBa_K1321352/annotation2415435/range2415435/1
component: BBa_K1321344

https://synbiohub.org/public/igem/BBa_K1321352/annotation2415435/range2415435/1
type: Range
persistent identity: range2415435
display id: range2415435
version: 1
top level: BBa_K1321352
direction: direction/reverse
start: 59
end: 567
orientation: inline

BBa_K1355001
type: ComponentDefinition
persistent identity: BBa K1355001
display id: BBa_K1355001
version: 1
was generated by: iGEM to SBOL conversion
description: Regulation and transport of mercury ions
created: 2014-10-05T11:00:00Z
modified: 2015-07-23T04:05:30Z
mutable provenance: This construction is based on sequences present in the O26-CRL plasmid found in Escherichia coli O26. We added a reverse tryptophan operon terminator to ensure transcription termination of the merR messenger RNA. However we didn???t add transcription terminator after the merP gene, aiming connect this biobrick with others, enabling various functions related to mercury
owned by: james
owned by: myers
mutable description: We call this part an ???Essential biobrick??? designed to be the key piece of any genetic construction related to mercury, targeting both a biosensor as a bioaccumulative. This biobrick has bidirectional promoter, the regulator MerR with a terminator reverse from the tryptophan operon and a forward MerP and MerT transporters.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1731_
m user id: 4206
owner id: 15499
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: If is desirable to accumulate mercury, assemble this biobrick with a good RBS, a coding sequence of a peptide having affinity to metal and a terminator! Its function will be regulated by presence of mercury through MerR regulator. The bacteria will carry the mercury to cytoplasm until it is linked to the peptide having affinity to the metal, making a inactive mercury. Is not it amazing? This is the strategy that we use in our project! The so called ???Essential Biobrick??? was connected to biobrick for bioremediation, bioaccumulation and bio-detection!
star: false
creator: Luna Barroco de Lacerda
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: MerT
sequence annotation: RBS
sequence annotation: Bidirecional promoter
sequence annotation: MerR
sequence annotation: MerP
sequence annotation: Trp Terminator
sequence: https://synbiohub.org/public/igem/BBa_K1355001_sequence/1

MerT
type: SequenceAnnotation
persistent identity: annotation2415141
display id: annotation2415141
version: 1
top level: BBa_K1355001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1355001/annotation2415141/range2415141/1

https://synbiohub.org/public/igem/BBa_K1355001/annotation2415141/range2415141/1
type: Range
persistent identity: range2415141
display id: range2415141
version: 1
top level: BBa_K1355001
direction: direction/forward
start: 551
end: 900
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2415749
display id: annotation2415749
version: 1
top level: BBa_K1355001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1355001/annotation2415749/range2415749/1

https://synbiohub.org/public/igem/BBa_K1355001/annotation2415749/range2415749/1
type: Range
persistent identity: range2415749
display id: range2415749
version: 1
top level: BBa_K1355001
direction: direction/reverse
start: 490
end: 496
orientation: inline

Bidirecional promoter
type: SequenceAnnotation
persistent identity: annotation2415127
display id: annotation2415127
version: 1
top level: BBa_K1355001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1355001/annotation2415127/range2415127/1

https://synbiohub.org/public/igem/BBa_K1355001/annotation2415127/range2415127/1
type: Range
persistent identity: range2415127
display id: range2415127
version: 1
top level: BBa_K1355001
direction: direction/forward
start: 486
end: 514
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2415752
display id: annotation2415752
version: 1
top level: BBa_K1355001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1355001/annotation2415752/range2415752/1

https://synbiohub.org/public/igem/BBa_K1355001/annotation2415752/range2415752/1
type: Range
persistent identity: range2415752
display id: range2415752
version: 1
top level: BBa_K1355001
direction: direction/forward
start: 537
end: 543
orientation: inline

MerR
type: SequenceAnnotation
persistent identity: annotation2415126
display id: annotation2415126
version: 1
top level: BBa_K1355001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1355001/annotation2415126/range2415126/1

https://synbiohub.org/public/igem/BBa_K1355001/annotation2415126/range2415126/1
type: Range
persistent identity: range2415126
display id: range2415126
version: 1
top level: BBa_K1355001
direction: direction/reverse
start: 44
end: 480
orientation: inline

Bidirecional promoter
type: SequenceAnnotation
persistent identity: annotation2415134
display id: annotation2415134
version: 1
top level: BBa_K1355001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1355001/annotation2415134/range2415134/1

https://synbiohub.org/public/igem/BBa_K1355001/annotation2415134/range2415134/1
type: Range
persistent identity: range2415134
display id: range2415134
version: 1
top level: BBa_K1355001
direction: direction/reverse
start: 510
end: 538
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2415143
display id: annotation2415143
version: 1
top level: BBa_K1355001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1355001/annotation2415143/range2415143/1

https://synbiohub.org/public/igem/BBa_K1355001/annotation2415143/range2415143/1
type: Range
persistent identity: range2415143
display id: range2415143
version: 1
top level: BBa_K1355001
direction: direction/forward
start: 901
end: 907
orientation: inline

MerP
type: SequenceAnnotation
persistent identity: annotation2415155
display id: annotation2415155
version: 1
top level: BBa_K1355001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1355001/annotation2415155/range2415155/1

https://synbiohub.org/public/igem/BBa_K1355001/annotation2415155/range2415155/1
type: Range
persistent identity: range2415155
display id: range2415155
version: 1
top level: BBa_K1355001
direction: direction/forward
start: 914
end: 1189
orientation: inline

Trp Terminator
type: SequenceAnnotation
persistent identity: annotation2397153
display id: annotation2397153
version: 1
top level: BBa_K1355001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1355001/annotation2397153/range2397153/1

https://synbiohub.org/public/igem/BBa_K1355001/annotation2397153/range2397153/1
type: Range
persistent identity: range2397153
display id: range2397153
version: 1
top level: BBa_K1355001
direction: direction/reverse
start: 1
end: 43
orientation: inline

BBa_K1321338
type: ComponentDefinition
persistent identity: BBa K1321338
display id: BBa_K1321338
version: 1
was generated by: iGEM to SBOL conversion
description: T7 Expression vector
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:09:53Z
mutable provenance: asdf
owned by: james
owned by: myers
mutable description: asdfasdf
discontinued: false
dominant: false
group u list: _1696_
m user id: 0
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: asdf
star: false
creator: Chris N Micklem
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_I719005
component: BBa_B0034
sequence annotation: BBa_I719005
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1321338_sequence/1

BBa_I719005
type: Component
persistent identity: component2402299
display id: component2402299
version: 1
top level: BBa_K1321338
access: public
definition: pT7

BBa_B0034
type: Component
persistent identity: component2402301
display id: component2402301
version: 1
top level: BBa_K1321338
access: public
definition: BBa_B0034

BBa_I719005
type: SequenceAnnotation
persistent identity: annotation2402299
display id: annotation2402299
version: 1
top level: BBa_K1321338
location: https://synbiohub.org/public/igem/BBa_K1321338/annotation2402299/range2402299/1
component: BBa_I719005

https://synbiohub.org/public/igem/BBa_K1321338/annotation2402299/range2402299/1
type: Range
persistent identity: range2402299
display id: range2402299
version: 1
top level: BBa_K1321338
direction: direction/reverse
start: 1
end: 23
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2402301
display id: annotation2402301
version: 1
top level: BBa_K1321338
location: https://synbiohub.org/public/igem/BBa_K1321338/annotation2402301/range2402301/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1321338/annotation2402301/range2402301/1
type: Range
persistent identity: range2402301
display id: range2402301
version: 1
top level: BBa_K1321338
direction: direction/reverse
start: 32
end: 43
orientation: inline

PbrR
type: ComponentDefinition
persistent identity: BBa K1701001
display id: BBa_K1701001
version: 1
was generated by: iGEM to SBOL conversion
description: PbrR
created: 2015-09-07T11:00:00Z
modified: 2016-01-11T03:17:03Z
mutable provenance: http://www.ncbi.nlm.nih.gov/protein/56130720
owned by: james
owned by: myers
mutable description: A lead-specific binding protein, PbrR, and promoter pbr from the lead resistance operon, pbr, of Cupriavidus metallidurans CH34 have shown highly sensitive and selective whole-cell detection of lead ions.
discontinued: false
dominant: false
experience: experience/Works
group u list: _2119_
m user id: 4206
owner id: 21319
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Wei Wei
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1701001_sequence/1

BBa_J04500
type: ComponentDefinition
persistent identity: BBa J04500
display id: BBa_J04500
version: 1
was generated by: iGEM to SBOL conversion
description: IPTG inducible promoter with RBS
created: 2005-06-08T11:00:00Z
modified: 2015-08-31T04:08:14Z
mutable provenance: Davidson Synth-Aces
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: R0010.B0034
discontinued: false
dominant: true
experience: experience/Works
group u list: _16_
m user id: 0
owner id: 326
owning group id: 16
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Kristen DeCelle
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_R0010
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_R0010
sequence: https://synbiohub.org/public/igem/BBa_J04500_sequence/1

BBa_R0010
type: Component
persistent identity: component1508149
display id: component1508149
version: 1
top level: BBa_J04500
access: public
definition: LacI

BBa_B0034
type: Component
persistent identity: component1508159
display id: component1508159
version: 1
top level: BBa_J04500
access: public
definition: BBa_B0034

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1508159
display id: annotation1508159
version: 1
top level: BBa_J04500
location: https://synbiohub.org/public/igem/BBa_J04500/annotation1508159/range1508159/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J04500/annotation1508159/range1508159/1
type: Range
persistent identity: range1508159
display id: range1508159
version: 1
top level: BBa_J04500
direction: direction/forward
start: 209
end: 220
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1508149
display id: annotation1508149
version: 1
top level: BBa_J04500
location: https://synbiohub.org/public/igem/BBa_J04500/annotation1508149/range1508149/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_J04500/annotation1508149/range1508149/1
type: Range
persistent identity: range1508149
display id: range1508149
version: 1
top level: BBa_J04500
direction: direction/forward
start: 1
end: 200
orientation: inline

CBDcex(J61
type: ComponentDefinition
persistent identity: BBa K863104
display id: BBa_K863104
version: 1
was generated by: iGEM to SBOL conversion
description: Cellulose binding Domain from C. Fimi with const. Promotor and GFP
created: 2012-09-20T11:00:00Z
modified: 2015-06-17T01:19:39Z
mutable provenance: different
owned by: james
owned by: myers
mutable description: Cellulose binding Domain from Cellulomonas Fimi with const. Promotor J61101 and Reporter GFP
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 4206
owner id: 13080
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: a switch to a const. Promoter
star: false
creator: Moritz M??ller
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: BBa_K863121
sequence annotation: Exoglucanase
sequence annotation: BBa_J23100
sequence annotation: BBa_E0040
sequence annotation: ATG
sequence annotation: GS-Linker
sequence annotation: Freiburg-Scar
sequence annotation: BBa_K863101
sequence annotation: BBa_J61101
sequence annotation: NgoMIV
sequence annotation: TAA
sequence annotation: His-Tag
sequence annotation: Cellulose binding domain (CBDcex)
sequence annotation: AgeI
sequence: https://synbiohub.org/public/igem/BBa_K863104_sequence/1

BBa_K863121
type: SequenceAnnotation
persistent identity: annotation2189493
display id: annotation2189493
version: 1
top level: CBDcex(J61
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189493/range2189493/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189493/range2189493/1
type: Range
persistent identity: range2189493
display id: range2189493
version: 1
top level: CBDcex(J61
direction: direction/forward
start: 404
end: 1144
orientation: inline

Exoglucanase
type: SequenceAnnotation
persistent identity: annotation2189488
display id: annotation2189488
version: 1
top level: CBDcex(J61
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189488/range2189488/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189488/range2189488/1
type: Range
persistent identity: range2189488
display id: range2189488
version: 1
top level: CBDcex(J61
direction: direction/forward
start: 71
end: 82
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2189483
display id: annotation2189483
version: 1
top level: CBDcex(J61
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189483/range2189483/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189483/range2189483/1
type: Range
persistent identity: range2189483
display id: range2189483
version: 1
top level: CBDcex(J61
direction: direction/forward
start: 1
end: 35
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2189494
display id: annotation2189494
version: 1
top level: CBDcex(J61
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189494/range2189494/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189494/range2189494/1
type: Range
persistent identity: range2189494
display id: range2189494
version: 1
top level: CBDcex(J61
direction: direction/forward
start: 404
end: 1117
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2189485
display id: annotation2189485
version: 1
top level: CBDcex(J61
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189485/range2189485/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189485/range2189485/1
type: Range
persistent identity: range2189485
display id: range2189485
version: 1
top level: CBDcex(J61
direction: direction/forward
start: 62
end: 64
orientation: inline

GS-Linker
type: SequenceAnnotation
persistent identity: annotation2189491
display id: annotation2189491
version: 1
top level: CBDcex(J61
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189491/range2189491/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189491/range2189491/1
type: Range
persistent identity: range2189491
display id: range2189491
version: 1
top level: CBDcex(J61
direction: direction/forward
start: 392
end: 397
orientation: inline

Exoglucanase
type: SequenceAnnotation
persistent identity: annotation2189490
display id: annotation2189490
version: 1
top level: CBDcex(J61
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189490/range2189490/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189490/range2189490/1
type: Range
persistent identity: range2189490
display id: range2189490
version: 1
top level: CBDcex(J61
direction: direction/forward
start: 383
end: 391
orientation: inline

Freiburg-Scar
type: SequenceAnnotation
persistent identity: annotation2189492
display id: annotation2189492
version: 1
top level: CBDcex(J61
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189492/range2189492/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189492/range2189492/1
type: Range
persistent identity: range2189492
display id: range2189492
version: 1
top level: CBDcex(J61
direction: direction/forward
start: 398
end: 403
orientation: inline

BBa_K863101
type: SequenceAnnotation
persistent identity: annotation2189486
display id: annotation2189486
version: 1
top level: CBDcex(J61
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189486/range2189486/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189486/range2189486/1
type: Range
persistent identity: range2189486
display id: range2189486
version: 1
top level: CBDcex(J61
direction: direction/forward
start: 62
end: 397
orientation: inline

BBa_J61101
type: SequenceAnnotation
persistent identity: annotation2189484
display id: annotation2189484
version: 1
top level: CBDcex(J61
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189484/range2189484/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189484/range2189484/1
type: Range
persistent identity: range2189484
display id: range2189484
version: 1
top level: CBDcex(J61
direction: direction/forward
start: 44
end: 55
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2189487
display id: annotation2189487
version: 1
top level: CBDcex(J61
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189487/range2189487/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189487/range2189487/1
type: Range
persistent identity: range2189487
display id: range2189487
version: 1
top level: CBDcex(J61
direction: direction/forward
start: 65
end: 70
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2189497
display id: annotation2189497
version: 1
top level: CBDcex(J61
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189497/range2189497/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189497/range2189497/1
type: Range
persistent identity: range2189497
display id: range2189497
version: 1
top level: CBDcex(J61
direction: direction/forward
start: 1142
end: 1144
orientation: inline

His-Tag
type: SequenceAnnotation
persistent identity: annotation2189495
display id: annotation2189495
version: 1
top level: CBDcex(J61
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189495/range2189495/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189495/range2189495/1
type: Range
persistent identity: range2189495
display id: range2189495
version: 1
top level: CBDcex(J61
direction: direction/forward
start: 1118
end: 1135
orientation: inline

Cellulose binding domain (CBDcex)
type: SequenceAnnotation
persistent identity: annotation2189489
display id: annotation2189489
version: 1
top level: CBDcex(J61
role: feature/protein
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189489/range2189489/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189489/range2189489/1
type: Range
persistent identity: range2189489
display id: range2189489
version: 1
top level: CBDcex(J61
direction: direction/forward
start: 83
end: 382
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2189496
display id: annotation2189496
version: 1
top level: CBDcex(J61
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189496/range2189496/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189496/range2189496/1
type: Range
persistent identity: range2189496
display id: range2189496
version: 1
top level: CBDcex(J61
direction: direction/forward
start: 1136
end: 1141
orientation: inline

BBa_K1321122
type: ComponentDefinition
persistent identity: BBa K1321122
display id: BBa_K1321122
version: 1
was generated by: iGEM to SBOL conversion
description: Smt fused to CBDcex
created: 2014-10-08T11:00:00Z
modified: 2015-06-17T12:22:45Z
mutable provenance: GATC
owned by: james
owned by: myers
mutable description: GATC
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20961
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: GATC
star: false
creator: Gabriella Santosa
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_B0105
component: BBa_K1321011
component: BBa_K1321003
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321011
sequence annotation: BBa_K1321003
sequence: https://synbiohub.org/public/igem/BBa_K1321122_sequence/1

BBa_B0105
type: Component
persistent identity: component2414990
display id: component2414990
version: 1
top level: BBa_K1321122
access: public
definition: Scar 25

BBa_K1321011
type: Component
persistent identity: component2414988
display id: component2414988
version: 1
top level: BBa_K1321122
access: public
definition: BBa_K1321011

BBa_K1321003
type: Component
persistent identity: component2414991
display id: component2414991
version: 1
top level: BBa_K1321122
access: public
definition: BBa_K1321003

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2414990
display id: annotation2414990
version: 1
top level: BBa_K1321122
location: https://synbiohub.org/public/igem/BBa_K1321122/annotation2414990/range2414990/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321122/annotation2414990/range2414990/1
type: Range
persistent identity: range2414990
display id: range2414990
version: 1
top level: BBa_K1321122
direction: direction/reverse
start: 166
end: 171
orientation: inline

BBa_K1321011
type: SequenceAnnotation
persistent identity: annotation2414988
display id: annotation2414988
version: 1
top level: BBa_K1321122
location: https://synbiohub.org/public/igem/BBa_K1321122/annotation2414988/range2414988/1
component: BBa_K1321011

https://synbiohub.org/public/igem/BBa_K1321122/annotation2414988/range2414988/1
type: Range
persistent identity: range2414988
display id: range2414988
version: 1
top level: BBa_K1321122
direction: direction/reverse
start: 1
end: 165
orientation: inline

BBa_K1321003
type: SequenceAnnotation
persistent identity: annotation2414991
display id: annotation2414991
version: 1
top level: BBa_K1321122
location: https://synbiohub.org/public/igem/BBa_K1321122/annotation2414991/range2414991/1
component: BBa_K1321003

https://synbiohub.org/public/igem/BBa_K1321122/annotation2414991/range2414991/1
type: Range
persistent identity: range2414991
display id: range2414991
version: 1
top level: BBa_K1321122
direction: direction/reverse
start: 172
end: 498
orientation: inline

BBa_K1460008
type: ComponentDefinition
persistent identity: BBa K1460008
display id: BBa_K1460008
version: 1
was generated by: iGEM to SBOL conversion
description: Anderson Promoter + CBP4 + pT7 + GST-CRS5 + Ter
created: 2014-08-25T11:00:00Z
modified: 2016-01-13T10:19:42Z
mutable provenance: This part is a composite of the parts BBa_K1460005 and BBa_K1460001.
owned by: james
owned by: myers
mutable description: This composite part functionally codes for the putative lead transporter CBP4 as well as the metallothioniein CRS5 fused to glutathione-S-transferase. This composite part should act to transport lead ions into the engineered cell where they will be sequestered by the metallothionein allowing for Pb2+ removal from contaminated water.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1839_
m user id: 4206
owner id: 15649
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: Expression of GST-CRS5 is controlled by the bacteriophage T7 promoter, so part should be combined with a host organism expressing T7 polymerase (such as E. coli BL21-AI from Life Technologies).
star: false
creator: Eric Holmes
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1460005
component: BBa_K1460001
sequence annotation: BBa_K1460001
sequence annotation: BBa_K1460005
sequence: https://synbiohub.org/public/igem/BBa_K1460008_sequence/1

BBa_K1460005
type: Component
persistent identity: component2381582
display id: component2381582
version: 1
top level: BBa_K1460008
access: public
definition: BBa_K1460005

BBa_K1460001
type: Component
persistent identity: component2381589
display id: component2381589
version: 1
top level: BBa_K1460008
access: public
definition: BBa_K1460001

BBa_K1460001
type: SequenceAnnotation
persistent identity: annotation2381589
display id: annotation2381589
version: 1
top level: BBa_K1460008
location: https://synbiohub.org/public/igem/BBa_K1460008/annotation2381589/range2381589/1
component: BBa_K1460001

https://synbiohub.org/public/igem/BBa_K1460008/annotation2381589/range2381589/1
type: Range
persistent identity: range2381589
display id: range2381589
version: 1
top level: BBa_K1460008
direction: direction/reverse
start: 2198
end: 3225
orientation: inline

BBa_K1460005
type: SequenceAnnotation
persistent identity: annotation2381582
display id: annotation2381582
version: 1
top level: BBa_K1460008
location: https://synbiohub.org/public/igem/BBa_K1460008/annotation2381582/range2381582/1
component: BBa_K1460005

https://synbiohub.org/public/igem/BBa_K1460008/annotation2381582/range2381582/1
type: Range
persistent identity: range2381582
display id: range2381582
version: 1
top level: BBa_K1460008
direction: direction/reverse
start: 1
end: 2189
orientation: inline

BBa_K1980012
type: ComponentDefinition
persistent identity: BBa K1980012
display id: BBa_K1980012
version: 1
was generated by: iGEM to SBOL conversion
description: pCopA MymT sfGFP with divergent CueR
created: 2016-10-10T11:00:00Z
modified: 2016-10-23T07:47:17Z
mutable provenance: E. coli TB
owned by: james
owned by: myers
mutable description: pCopA MymT sfGFP with plasmid constitutively expressed CueR
discontinued: false
dominant: false
experience: experience/Works
group u list: _2447_
m user id: 0
m user id: 29607
owner id: 29607
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: dsjsdgydahjah
star: false
creator: Sam Garforth
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K1980003
component: BBa_K1980006
sequence annotation: BBa_K1980003
sequence annotation: BBa_K1980006
sequence: https://synbiohub.org/public/igem/BBa_K1980012_sequence/1

BBa_K1980003
type: Component
persistent identity: component2493046
display id: component2493046
version: 1
top level: BBa_K1980012
access: public
definition: MymTsfGFP

BBa_K1980006
type: Component
persistent identity: component2493045
display id: component2493045
version: 1
top level: BBa_K1980012
access: public
definition: BBa_K1980006

BBa_K1980003
type: SequenceAnnotation
persistent identity: annotation2493046
display id: annotation2493046
version: 1
top level: BBa_K1980012
location: https://synbiohub.org/public/igem/BBa_K1980012/annotation2493046/range2493046/1
component: BBa_K1980003

https://synbiohub.org/public/igem/BBa_K1980012/annotation2493046/range2493046/1
type: Range
persistent identity: range2493046
display id: range2493046
version: 1
top level: BBa_K1980012
direction: direction/reverse
start: 581
end: 1492
orientation: inline

BBa_K1980006
type: SequenceAnnotation
persistent identity: annotation2493045
display id: annotation2493045
version: 1
top level: BBa_K1980012
location: https://synbiohub.org/public/igem/BBa_K1980012/annotation2493045/range2493045/1
component: BBa_K1980006

https://synbiohub.org/public/igem/BBa_K1980012/annotation2493045/range2493045/1
type: Range
persistent identity: range2493045
display id: range2493045
version: 1
top level: BBa_K1980012
direction: direction/reverse
start: 1
end: 574
orientation: inline

BBa_K1321343
type: ComponentDefinition
persistent identity: BBa K1321343
display id: BBa_K1321343
version: 1
was generated by: iGEM to SBOL conversion
description: NiBP fused to CBDclos in RFC 25
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:26:16Z
mutable provenance: asdf
owned by: james
owned by: myers
mutable description: adsdf
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: asdf
star: false
creator: Chris N Micklem
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_B0105
component: BBa_K1321009
component: BBa_K1321002
sequence annotation: BBa_K1321002
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321009
sequence: https://synbiohub.org/public/igem/BBa_K1321343_sequence/1

BBa_B0105
type: Component
persistent identity: component2415100
display id: component2415100
version: 1
top level: BBa_K1321343
access: public
definition: Scar 25

BBa_K1321009
type: Component
persistent identity: component2415098
display id: component2415098
version: 1
top level: BBa_K1321343
access: public
definition: BBa_K1321009

BBa_K1321002
type: Component
persistent identity: component2415101
display id: component2415101
version: 1
top level: BBa_K1321343
access: public
definition: BBa_K1321002

BBa_K1321002
type: SequenceAnnotation
persistent identity: annotation2415101
display id: annotation2415101
version: 1
top level: BBa_K1321343
location: https://synbiohub.org/public/igem/BBa_K1321343/annotation2415101/range2415101/1
component: BBa_K1321002

https://synbiohub.org/public/igem/BBa_K1321343/annotation2415101/range2415101/1
type: Range
persistent identity: range2415101
display id: range2415101
version: 1
top level: BBa_K1321343
direction: direction/reverse
start: 183
end: 482
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2415100
display id: annotation2415100
version: 1
top level: BBa_K1321343
location: https://synbiohub.org/public/igem/BBa_K1321343/annotation2415100/range2415100/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321343/annotation2415100/range2415100/1
type: Range
persistent identity: range2415100
display id: range2415100
version: 1
top level: BBa_K1321343
direction: direction/reverse
start: 177
end: 182
orientation: inline

BBa_K1321009
type: SequenceAnnotation
persistent identity: annotation2415098
display id: annotation2415098
version: 1
top level: BBa_K1321343
location: https://synbiohub.org/public/igem/BBa_K1321343/annotation2415098/range2415098/1
component: BBa_K1321009

https://synbiohub.org/public/igem/BBa_K1321343/annotation2415098/range2415098/1
type: Range
persistent identity: range2415098
display id: range2415098
version: 1
top level: BBa_K1321343
direction: direction/reverse
start: 1
end: 176
orientation: inline

BBa_K1460004
type: ComponentDefinition
persistent identity: BBa K1460004
display id: BBa_K1460004
version: 1
was generated by: iGEM to SBOL conversion
description: Anderson Promoter + MerT + MerP + ter
created: 2014-08-25T11:00:00Z
modified: 2016-01-13T10:25:46Z
mutable provenance: Anderson promoter is that of part BBa_J23102. Terminator is that of part BBa_B1006. merT/merP genes are naturally found in Pseudomonas aeruginosa. This part was synthesized by GenScript in the vector pUC57. The part was then transferred into pSB1C3 using standard cloning procedures.
owned by: james
owned by: myers
mutable description: This is a functional coding sequence for the mercury transport proteins merT/merP from P. aeruginosa. This part will result in mercury intolerance due to greater mercury transport into engineered cells.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1839_
m user id: 4206
owner id: 15649
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The Anderson promoter provides strong constitutive expression of merT/merP in E. coli
star: false
creator: Eric Holmes
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_J61101
sequence annotation: BBa_J23102
sequence annotation: BBa_B1006
sequence annotation: merP
sequence annotation: merT
sequence: https://synbiohub.org/public/igem/BBa_K1460004_sequence/1

BBa_J61101
type: SequenceAnnotation
persistent identity: annotation2381483
display id: annotation2381483
version: 1
top level: BBa_K1460004
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1460004/annotation2381483/range2381483/1

https://synbiohub.org/public/igem/BBa_K1460004/annotation2381483/range2381483/1
type: Range
persistent identity: range2381483
display id: range2381483
version: 1
top level: BBa_K1460004
direction: direction/forward
start: 43
end: 56
orientation: inline

BBa_J23102
type: SequenceAnnotation
persistent identity: annotation2381484
display id: annotation2381484
version: 1
top level: BBa_K1460004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1460004/annotation2381484/range2381484/1

https://synbiohub.org/public/igem/BBa_K1460004/annotation2381484/range2381484/1
type: Range
persistent identity: range2381484
display id: range2381484
version: 1
top level: BBa_K1460004
direction: direction/forward
start: 1
end: 35
orientation: inline

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2381487
display id: annotation2381487
version: 1
top level: BBa_K1460004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1460004/annotation2381487/range2381487/1

https://synbiohub.org/public/igem/BBa_K1460004/annotation2381487/range2381487/1
type: Range
persistent identity: range2381487
display id: range2381487
version: 1
top level: BBa_K1460004
direction: direction/forward
start: 702
end: 740
orientation: inline

merP
type: SequenceAnnotation
persistent identity: annotation2381486
display id: annotation2381486
version: 1
top level: BBa_K1460004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1460004/annotation2381486/range2381486/1

https://synbiohub.org/public/igem/BBa_K1460004/annotation2381486/range2381486/1
type: Range
persistent identity: range2381486
display id: range2381486
version: 1
top level: BBa_K1460004
direction: direction/forward
start: 426
end: 701
orientation: inline

merT
type: SequenceAnnotation
persistent identity: annotation2381485
display id: annotation2381485
version: 1
top level: BBa_K1460004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1460004/annotation2381485/range2381485/1

https://synbiohub.org/public/igem/BBa_K1460004/annotation2381485/range2381485/1
type: Range
persistent identity: range2381485
display id: range2381485
version: 1
top level: BBa_K1460004
direction: direction/forward
start: 63
end: 413
orientation: inline

BBa_K1321120
type: ComponentDefinition
persistent identity: BBa K1321120
display id: BBa_K1321120
version: 1
was generated by: iGEM to SBOL conversion
description: SmtA metallothionein fused to linker-dCBD
created: 2014-10-08T11:00:00Z
modified: 2015-06-17T12:22:14Z
mutable provenance: GATC
owned by: james
owned by: myers
mutable description: GATC
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20961
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: GATC
star: false
creator: Gabriella Santosa
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321011
component: BBa_B0105
component: BBa_K1321340
sequence annotation: BBa_K1321011
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321340
sequence: https://synbiohub.org/public/igem/BBa_K1321120_sequence/1

BBa_K1321011
type: Component
persistent identity: component2414675
display id: component2414675
version: 1
top level: BBa_K1321120
access: public
definition: BBa_K1321011

BBa_B0105
type: Component
persistent identity: component2414677
display id: component2414677
version: 1
top level: BBa_K1321120
access: public
definition: Scar 25

BBa_K1321340
type: Component
persistent identity: component2414679
display id: component2414679
version: 1
top level: BBa_K1321120
access: public
definition: BBa_K1321340

BBa_K1321011
type: SequenceAnnotation
persistent identity: annotation2414675
display id: annotation2414675
version: 1
top level: BBa_K1321120
location: https://synbiohub.org/public/igem/BBa_K1321120/annotation2414675/range2414675/1
component: BBa_K1321011

https://synbiohub.org/public/igem/BBa_K1321120/annotation2414675/range2414675/1
type: Range
persistent identity: range2414675
display id: range2414675
version: 1
top level: BBa_K1321120
direction: direction/reverse
start: 1
end: 165
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2414677
display id: annotation2414677
version: 1
top level: BBa_K1321120
location: https://synbiohub.org/public/igem/BBa_K1321120/annotation2414677/range2414677/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321120/annotation2414677/range2414677/1
type: Range
persistent identity: range2414677
display id: range2414677
version: 1
top level: BBa_K1321120
direction: direction/reverse
start: 166
end: 171
orientation: inline

BBa_K1321340
type: SequenceAnnotation
persistent identity: annotation2414679
display id: annotation2414679
version: 1
top level: BBa_K1321120
location: https://synbiohub.org/public/igem/BBa_K1321120/annotation2414679/range2414679/1
component: BBa_K1321340

https://synbiohub.org/public/igem/BBa_K1321120/annotation2414679/range2414679/1
type: Range
persistent identity: range2414679
display id: range2414679
version: 1
top level: BBa_K1321120
direction: direction/reverse
start: 172
end: 537
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: BBa_R0010
sequence annotation: end of LacI coding region (inactive)
sequence annotation: -10
sequence annotation: start
sequence annotation: LacI binding site
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
role: feature/start
role: SO:0000318
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

MymT
type: ComponentDefinition
persistent identity: BBa K1980002
display id: BBa_K1980002
version: 1
was generated by: iGEM to SBOL conversion
description: MymT
created: 2016-10-10T11:00:00Z
modified: 2016-10-23T03:09:02Z
mutable provenance: Mycobacterium tuberculosis
owned by: james
owned by: myers
mutable description: MymT is a small prokaryotic copper metallothein discovered in Mycobacterium tuberculosis. It is believed that the protein may help the bacterium survive copper toxicity, though deleting the gene had no effect on pathogenicity in mice. It can bind up to 7 copper ions but has a preference for 4-6. Our version has a C terminal hexahistidine tag for purification and has been codon optimised for E. coli.
discontinued: false
dominant: false
experience: experience/Issues
group u list: _2447_
m user id: 29607
owner id: 29607
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: This protein has a C terminal hexahistidine tag for purification and has been codon optimised for E. coli.
star: false
creator: Sam Garforth
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: MymT
sequence annotation: Double stop
sequence annotation: Hexahistidine
sequence: https://synbiohub.org/public/igem/BBa_K1980002_sequence/1

MymT
type: SequenceAnnotation
persistent identity: annotation2495239
display id: annotation2495239
version: 1
top level: MymT
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1980002/annotation2495239/range2495239/1

https://synbiohub.org/public/igem/BBa_K1980002/annotation2495239/range2495239/1
type: Range
persistent identity: range2495239
display id: range2495239
version: 1
top level: MymT
direction: direction/forward
start: 1
end: 159
orientation: inline

Double stop
type: SequenceAnnotation
persistent identity: annotation2495241
display id: annotation2495241
version: 1
top level: MymT
role: feature/stop
role: SO:0000319
location: https://synbiohub.org/public/igem/BBa_K1980002/annotation2495241/range2495241/1

https://synbiohub.org/public/igem/BBa_K1980002/annotation2495241/range2495241/1
type: Range
persistent identity: range2495241
display id: range2495241
version: 1
top level: MymT
direction: direction/forward
start: 178
end: 183
orientation: inline

Hexahistidine
type: SequenceAnnotation
persistent identity: annotation2495240
display id: annotation2495240
version: 1
top level: MymT
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1980002/annotation2495240/range2495240/1

https://synbiohub.org/public/igem/BBa_K1980002/annotation2495240/range2495240/1
type: Range
persistent identity: range2495240
display id: range2495240
version: 1
top level: MymT
direction: direction/forward
start: 160
end: 177
orientation: inline

BBa_K1979001
type: ComponentDefinition
persistent identity: BBa K1979001
display id: BBa_K1979001
version: 1
was generated by: iGEM to SBOL conversion
description: CBD (cbh2)
created: 2016-08-19T11:00:00Z
modified: 2016-08-24T10:46:39Z
mutable provenance: The sequence is retrieved from Genbank.
owned by: james
owned by: myers
mutable description: The sequence codes for cellulose binding domain cbh2 in prokaryotic cells.
discontinued: false
dominant: false
group u list: _2446_
m user id: 29797
owner id: 29797
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Restriction sites Xho I and Bamh I are added.
star: false
creator: MA Xinyi
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: CBD cbh2
sequence: https://synbiohub.org/public/igem/BBa_K1979001_sequence/1

CBD cbh2
type: SequenceAnnotation
persistent identity: annotation2481557
display id: annotation2481557
version: 1
top level: BBa_K1979001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1979001/annotation2481557/range2481557/1

https://synbiohub.org/public/igem/BBa_K1979001/annotation2481557/range2481557/1
type: Range
persistent identity: range2481557
display id: range2481557
version: 1
top level: BBa_K1979001
direction: direction/forward
start: 1
end: 147
orientation: inline

BFR
type: ComponentDefinition
persistent identity: BBa K1438000
display id: BBa_K1438000
version: 1
was generated by: iGEM to SBOL conversion
description: Bacterioferritin (BFR)
created: 2014-09-12T11:00:00Z
modified: 2015-07-22T02:12:09Z
mutable provenance: Bacterioferritin from E. coli Nissle 1917 genomic DNA. Amplified by using PCR and cloned into pQE80L
owned by: james
owned by: myers
mutable description: Bacterioferritins are the E. coli cells natural iron storage proteins. These hollow nearly spherical protein shells detoxify the cell by sequestering excessive iron and forming Iron(III)hydroxid-oxide particels. Bacterioferritin is an heam containing bacterial ferritin. Each heme is bound in a pocked formed by the interface between a pair of symmetry-related subunits [1]. However, it was investigated that these heme groups may be involved in the release of iron out of the ferritin iron core by forming an heme-mediated electron transfer to reduce immobilized Fe3+ to more soluble Fe2+. [1] Frolow F, Kalb AJ, Yariv J. Structure of a unique twofold symmetric haem-binding site. Nat Struct Biol. 1994 Jul;1(7):453-60. PubMed PMID: 7664064. [2] Yao H, Wang Y, Lovell S, Kumar R, Ruvinsky AM, Battaile KP, Vakser IA, Rivera M. The structure of the BfrB-Bfd complex reveals protein-protein interactions enabling iron release from bacterioferritin. J Am Chem Soc. 2012 Aug 15;134(32):13470-81. doi: 10.1021/ja305180n. Epub 2012 Aug 1. PubMed PMID: 22812654; PubMed Central PMCID: PMC3428730.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1816_
m user id: 4206
owner id: 19714
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: For any metal nanoparticles built inside of BFR it would be beneficary to remove any heme groups in order to prevent unloading of BFR by heme-mediated electron transfer.
star: false
creator: Johann Bauerfeind
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop codon
sequence annotation: start codon
sequence annotation: His Tag
sequence annotation: BioBrick
sequence: https://synbiohub.org/public/igem/BBa_K1438000_sequence/1

stop codon
type: SequenceAnnotation
persistent identity: annotation2398526
display id: annotation2398526
version: 1
top level: BFR
role: feature/stop
role: SO:0000319
location: https://synbiohub.org/public/igem/BBa_K1438000/annotation2398526/range2398526/1

https://synbiohub.org/public/igem/BBa_K1438000/annotation2398526/range2398526/1
type: Range
persistent identity: range2398526
display id: range2398526
version: 1
top level: BFR
direction: direction/forward
start: 511
end: 513
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2383111
display id: annotation2383111
version: 1
top level: BFR
role: feature/start
role: SO:0000318
location: https://synbiohub.org/public/igem/BBa_K1438000/annotation2383111/range2383111/1

https://synbiohub.org/public/igem/BBa_K1438000/annotation2383111/range2383111/1
type: Range
persistent identity: range2383111
display id: range2383111
version: 1
top level: BFR
direction: direction/forward
start: 1
end: 3
orientation: inline

His Tag
type: SequenceAnnotation
persistent identity: annotation2398525
display id: annotation2398525
version: 1
top level: BFR
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1438000/annotation2398525/range2398525/1

https://synbiohub.org/public/igem/BBa_K1438000/annotation2398525/range2398525/1
type: Range
persistent identity: range2398525
display id: range2398525
version: 1
top level: BFR
direction: direction/forward
start: 13
end: 30
orientation: inline

BioBrick
type: SequenceAnnotation
persistent identity: annotation2398554
display id: annotation2398554
version: 1
top level: BFR
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1438000/annotation2398554/range2398554/1

https://synbiohub.org/public/igem/BBa_K1438000/annotation2398554/range2398554/1
type: Range
persistent identity: range2398554
display id: range2398554
version: 1
top level: BFR
direction: direction/forward
start: 1
end: 513
orientation: inline

BBa_I721002
type: ComponentDefinition
persistent identity: BBa I721002
display id: BBa_I721002
version: 1
was generated by: iGEM to SBOL conversion
description: Lead Binding Protein
created: 2007-10-25T11:00:00Z
modified: 2015-08-31T04:07:53Z
mutable provenance: ralstonia metallidurans
owned by: james
owned by: myers
mutable description: lead binding protein part 3
discontinued: false
dominant: false
experience: experience/None
group u list: _113_
m user id: 0
owner id: 1531
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: none
star: true
creator: Jeffrey Hofmann
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I721002_sequence/1

BBa_K1420004
type: ComponentDefinition
persistent identity: BBa K1420004
display id: BBa_K1420004
version: 1
was generated by: iGEM to SBOL conversion
description: merR family transcriptional regulator, regulatory protein for mer operon
created: 2014-10-08T11:00:00Z
modified: 2015-07-22T03:01:33Z
mutable provenance: Source organism: Serratia marcescens. Amplified from plasmid pDU1358, kindly donated by Anne O. Summers.
owned by: james
owned by: myers
mutable description: merR, located upstream of the rest of the mer genes, serves to regulate the operon. The MerR protein contains a C-terminal effector binding region that recognizes ionic mercury. Upon binding it, the protein???s N-terminal helix-turn-helix DNA binding domain can bind the merR promoter region to induce transcription of the operon. The effector binding region of the protein can vary allowing for great diversity in merR-like promoters that can respond to a variety of heavy metals as well as antibiotics and oxidative stress.
discontinued: false
dominant: false
experience: experience/None
group u list: _1798_
m user id: 4206
owner id: 17872
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: N/A
star: false
creator: Stephen C. Heinsch
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: merR
sequence: https://synbiohub.org/public/igem/BBa_K1420004_sequence/1

merR
type: SequenceAnnotation
persistent identity: annotation2422563
display id: annotation2422563
version: 1
top level: BBa_K1420004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1420004/annotation2422563/range2422563/1

https://synbiohub.org/public/igem/BBa_K1420004/annotation2422563/range2422563/1
type: Range
persistent identity: range2422563
display id: range2422563
version: 1
top level: BBa_K1420004
direction: direction/forward
start: 1
end: 435
orientation: inline

BBa_K1933011
type: ComponentDefinition
persistent identity: BBa K1933011
display id: BBa_K1933011
version: 1
was generated by: iGEM to SBOL conversion
description: INPNC fused to 6xHis tag without stop codon
created: 2016-10-16T11:00:00Z
modified: 2016-10-17T01:49:52Z
mutable provenance: INPNC derived from BBa_K811005
owned by: james
owned by: myers
mutable description: INPNC codes for N- and C- terminal domain of Ice-Nucleation Protein (INP) from Pseudomonas syringae , connected by a 6xHis tag to be easily identified by Western blotting[1]. This is used as an anchoring motif for the cell surface display of passengers. This domain does not have signal peptide for anchoring on the cell surface[2]. Surface displayed passengers retain enzyme activity even when it is linked to INPNC with 6xHis tag.
discontinued: false
dominant: false
experience: experience/None
group u list: _2400_
m user id: 23701
owner id: 23701
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: We omitted stop codon from BBa_K1933001 to use it for the registration of composite parts.
star: false
creator: Sora Matsumoto, Tomoki Uchino
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: 6xHis tag
sequence annotation: INPNC
sequence annotation: start codon
sequence: https://synbiohub.org/public/igem/BBa_K1933011_sequence/1

6xHis tag
type: SequenceAnnotation
persistent identity: annotation2514868
display id: annotation2514868
version: 1
top level: BBa_K1933011
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1933011/annotation2514868/range2514868/1

https://synbiohub.org/public/igem/BBa_K1933011/annotation2514868/range2514868/1
type: Range
persistent identity: range2514868
display id: range2514868
version: 1
top level: BBa_K1933011
direction: direction/forward
start: 958
end: 975
orientation: inline

INPNC
type: SequenceAnnotation
persistent identity: annotation2514867
display id: annotation2514867
version: 1
top level: BBa_K1933011
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1933011/annotation2514867/range2514867/1

https://synbiohub.org/public/igem/BBa_K1933011/annotation2514867/range2514867/1
type: Range
persistent identity: range2514867
display id: range2514867
version: 1
top level: BBa_K1933011
direction: direction/forward
start: 4
end: 957
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2514865
display id: annotation2514865
version: 1
top level: BBa_K1933011
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1933011/annotation2514865/range2514865/1

https://synbiohub.org/public/igem/BBa_K1933011/annotation2514865/range2514865/1
type: Range
persistent identity: range2514865
display id: range2514865
version: 1
top level: BBa_K1933011
direction: direction/forward
start: 1
end: 3
orientation: inline

BBa_K1438003
type: ComponentDefinition
persistent identity: BBa K1438003
display id: BBa_K1438003
version: 1
was generated by: iGEM to SBOL conversion
description: PPMT_GS_ATPCS
created: 2014-10-05T11:00:00Z
modified: 2015-07-22T02:14:35Z
mutable provenance: Arabidopsis thaliana (Columbia; leave) Pseudomonas Putida KT2240 (synthsized)
owned by: james
owned by: myers
mutable description: This Fusionprotein was obtained by fusion PPMT and ATPCSand connecting both through the Freiburg GS-Linker PPMT Pseudomonas Putida metallothionein ATPCS Arabidopsis thaliana Phytochelatin synthase
discontinued: false
dominant: false
group u list: _1816_
m user id: 4206
owner id: 19714
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Accodording to he paper Park et al the coexpression of both proteins leads into the formation of an inclusionbody like nanoparticle. Which leads to intercelluar synthesis of diverse nanoparticles.
star: false
creator: Johann Bauerfeind
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1438003_sequence/1

CP47His
type: ComponentDefinition
persistent identity: BBa K2127004
display id: BBa_K2127004
version: 1
was generated by: iGEM to SBOL conversion
description: CP-47-HIS TAG
created: 2016-10-13T11:00:00Z
modified: 2016-10-21T07:31:10Z
mutable provenance: CP-47-HIS TAG
owned by: james
owned by: myers
mutable description: CP-47-HIS TAG
discontinued: false
dominant: false
experience: experience/None
group u list: _2596_
m user id: 32415
owner id: 32736
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: CP-47-HIS TAG
star: false
creator: Mirat Sojitra
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: HisTag
sequence annotation: double stop codon
sequence annotation: CP47His
sequence: https://synbiohub.org/public/igem/BBa_K2127004_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2526359
display id: annotation2526359
version: 1
top level: CP47His
role: feature/start
role: SO:0000318
location: https://synbiohub.org/public/igem/BBa_K2127004/annotation2526359/range2526359/1

https://synbiohub.org/public/igem/BBa_K2127004/annotation2526359/range2526359/1
type: Range
persistent identity: range2526359
display id: range2526359
version: 1
top level: CP47His
direction: direction/forward
start: 1
end: 3
orientation: inline

HisTag
type: SequenceAnnotation
persistent identity: annotation2526361
display id: annotation2526361
version: 1
top level: CP47His
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K2127004/annotation2526361/range2526361/1

https://synbiohub.org/public/igem/BBa_K2127004/annotation2526361/range2526361/1
type: Range
persistent identity: range2526361
display id: range2526361
version: 1
top level: CP47His
direction: direction/forward
start: 1522
end: 1539
orientation: inline

double stop codon
type: SequenceAnnotation
persistent identity: annotation2526362
display id: annotation2526362
version: 1
top level: CP47His
role: feature/stop
role: SO:0000319
location: https://synbiohub.org/public/igem/BBa_K2127004/annotation2526362/range2526362/1

https://synbiohub.org/public/igem/BBa_K2127004/annotation2526362/range2526362/1
type: Range
persistent identity: range2526362
display id: range2526362
version: 1
top level: CP47His
direction: direction/forward
start: 1543
end: 1548
orientation: inline

CP47His
type: SequenceAnnotation
persistent identity: annotation2526360
display id: annotation2526360
version: 1
top level: CP47His
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K2127004/annotation2526360/range2526360/1

https://synbiohub.org/public/igem/BBa_K2127004/annotation2526360/range2526360/1
type: Range
persistent identity: range2526360
display id: range2526360
version: 1
top level: CP47His
direction: direction/forward
start: 1
end: 1548
orientation: inline

BBa_K1321114
type: ComponentDefinition
persistent identity: BBa K1321114
display id: BBa_K1321114
version: 1
was generated by: iGEM to SBOL conversion
description: CBDclos fused to phytochelatin (PC) EC20 with LacI promoter
created: 2014-10-08T11:00:00Z
modified: 2015-06-17T12:19:54Z
mutable provenance: CBD4+PhytoC with LacI
owned by: james
owned by: myers
mutable description: CBD4+PhytoC with LacI
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20780
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: CBD4+PhytoC with LacI
star: false
creator: Xenia Spencer-Milnes
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_B0105
component: BBa_K1321002
component: BBa_K1321005
component: BBa_K1319106
component: BBa_J04500
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321002
sequence annotation: BBa_K1321005
sequence annotation: BBa_K1319106
sequence annotation: BBa_J04500
sequence: https://synbiohub.org/public/igem/BBa_K1321114_sequence/1

BBa_B0105
type: Component
persistent identity: component2414934
display id: component2414934
version: 1
top level: BBa_K1321114
access: public
definition: Scar 25

BBa_K1321002
type: Component
persistent identity: component2414932
display id: component2414932
version: 1
top level: BBa_K1321114
access: public
definition: BBa_K1321002

BBa_K1321005
type: Component
persistent identity: component2414936
display id: component2414936
version: 1
top level: BBa_K1321114
access: public
definition: BBa_K1321005

BBa_K1319106
type: Component
persistent identity: component2414931
display id: component2414931
version: 1
top level: BBa_K1321114
access: public
definition: Scar25 ATG

BBa_J04500
type: Component
persistent identity: component2414929
display id: component2414929
version: 1
top level: BBa_K1321114
access: public
definition: BBa_J04500

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2414934
display id: annotation2414934
version: 1
top level: BBa_K1321114
location: https://synbiohub.org/public/igem/BBa_K1321114/annotation2414934/range2414934/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321114/annotation2414934/range2414934/1
type: Range
persistent identity: range2414934
display id: range2414934
version: 1
top level: BBa_K1321114
direction: direction/reverse
start: 536
end: 541
orientation: inline

BBa_K1321002
type: SequenceAnnotation
persistent identity: annotation2414932
display id: annotation2414932
version: 1
top level: BBa_K1321114
location: https://synbiohub.org/public/igem/BBa_K1321114/annotation2414932/range2414932/1
component: BBa_K1321002

https://synbiohub.org/public/igem/BBa_K1321114/annotation2414932/range2414932/1
type: Range
persistent identity: range2414932
display id: range2414932
version: 1
top level: BBa_K1321114
direction: direction/reverse
start: 236
end: 535
orientation: inline

BBa_K1321005
type: SequenceAnnotation
persistent identity: annotation2414936
display id: annotation2414936
version: 1
top level: BBa_K1321114
location: https://synbiohub.org/public/igem/BBa_K1321114/annotation2414936/range2414936/1
component: BBa_K1321005

https://synbiohub.org/public/igem/BBa_K1321114/annotation2414936/range2414936/1
type: Range
persistent identity: range2414936
display id: range2414936
version: 1
top level: BBa_K1321114
direction: direction/reverse
start: 542
end: 664
orientation: inline

BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2414931
display id: annotation2414931
version: 1
top level: BBa_K1321114
location: https://synbiohub.org/public/igem/BBa_K1321114/annotation2414931/range2414931/1
component: BBa_K1319106

https://synbiohub.org/public/igem/BBa_K1321114/annotation2414931/range2414931/1
type: Range
persistent identity: range2414931
display id: range2414931
version: 1
top level: BBa_K1321114
direction: direction/reverse
start: 221
end: 235
orientation: inline

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2414929
display id: annotation2414929
version: 1
top level: BBa_K1321114
location: https://synbiohub.org/public/igem/BBa_K1321114/annotation2414929/range2414929/1
component: BBa_J04500

https://synbiohub.org/public/igem/BBa_K1321114/annotation2414929/range2414929/1
type: Range
persistent identity: range2414929
display id: range2414929
version: 1
top level: BBa_K1321114
direction: direction/reverse
start: 1
end: 220
orientation: inline

BBa_K1321100
type: ComponentDefinition
persistent identity: BBa K1321100
display id: BBa_K1321100
version: 1
was generated by: iGEM to SBOL conversion
description: Phytochelatin (PC) EC20 fused to linker-dCBD with T7 promoter
created: 2014-10-08T11:00:00Z
modified: 2015-06-17T12:18:05Z
mutable provenance: Phytochelatin EC(20) +CBD2 with T7. TBC
owned by: james
owned by: myers
mutable description: Phytochelatin EC(20) +CBD2 with T7. TBC
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20780
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Phytochelatin EC(20) +CBD2 with T7. TBC
star: false
creator: Xenia Spencer-Milnes
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1319106
component: BBa_K1321340
component: BBa_K1321338
component: BBa_K1321005
component: BBa_B0105
sequence annotation: BBa_K1321005
sequence annotation: BBa_B0105
sequence annotation: BBa_K1319106
sequence annotation: BBa_K1321340
sequence annotation: BBa_K1321338
sequence: https://synbiohub.org/public/igem/BBa_K1321100_sequence/1

BBa_K1319106
type: Component
persistent identity: component2415249
display id: component2415249
version: 1
top level: BBa_K1321100
access: public
definition: Scar25 ATG

BBa_K1321340
type: Component
persistent identity: component2415255
display id: component2415255
version: 1
top level: BBa_K1321100
access: public
definition: BBa_K1321340

BBa_K1321338
type: Component
persistent identity: component2415247
display id: component2415247
version: 1
top level: BBa_K1321100
access: public
definition: BBa_K1321338

BBa_K1321005
type: Component
persistent identity: component2415251
display id: component2415251
version: 1
top level: BBa_K1321100
access: public
definition: BBa_K1321005

BBa_B0105
type: Component
persistent identity: component2415253
display id: component2415253
version: 1
top level: BBa_K1321100
access: public
definition: Scar 25

BBa_K1321005
type: SequenceAnnotation
persistent identity: annotation2415251
display id: annotation2415251
version: 1
top level: BBa_K1321100
location: https://synbiohub.org/public/igem/BBa_K1321100/annotation2415251/range2415251/1
component: BBa_K1321005

https://synbiohub.org/public/igem/BBa_K1321100/annotation2415251/range2415251/1
type: Range
persistent identity: range2415251
display id: range2415251
version: 1
top level: BBa_K1321100
direction: direction/reverse
start: 59
end: 181
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2415253
display id: annotation2415253
version: 1
top level: BBa_K1321100
location: https://synbiohub.org/public/igem/BBa_K1321100/annotation2415253/range2415253/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321100/annotation2415253/range2415253/1
type: Range
persistent identity: range2415253
display id: range2415253
version: 1
top level: BBa_K1321100
direction: direction/reverse
start: 182
end: 187
orientation: inline

BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2415249
display id: annotation2415249
version: 1
top level: BBa_K1321100
location: https://synbiohub.org/public/igem/BBa_K1321100/annotation2415249/range2415249/1
component: BBa_K1319106

https://synbiohub.org/public/igem/BBa_K1321100/annotation2415249/range2415249/1
type: Range
persistent identity: range2415249
display id: range2415249
version: 1
top level: BBa_K1321100
direction: direction/reverse
start: 44
end: 58
orientation: inline

BBa_K1321340
type: SequenceAnnotation
persistent identity: annotation2415255
display id: annotation2415255
version: 1
top level: BBa_K1321100
location: https://synbiohub.org/public/igem/BBa_K1321100/annotation2415255/range2415255/1
component: BBa_K1321340

https://synbiohub.org/public/igem/BBa_K1321100/annotation2415255/range2415255/1
type: Range
persistent identity: range2415255
display id: range2415255
version: 1
top level: BBa_K1321100
direction: direction/reverse
start: 188
end: 553
orientation: inline

BBa_K1321338
type: SequenceAnnotation
persistent identity: annotation2415247
display id: annotation2415247
version: 1
top level: BBa_K1321100
location: https://synbiohub.org/public/igem/BBa_K1321100/annotation2415247/range2415247/1
component: BBa_K1321338

https://synbiohub.org/public/igem/BBa_K1321100/annotation2415247/range2415247/1
type: Range
persistent identity: range2415247
display id: range2415247
version: 1
top level: BBa_K1321100
direction: direction/reverse
start: 1
end: 43
orientation: inline

BBa_K1321095
type: ComponentDefinition
persistent identity: BBa K1321095
display id: BBa_K1321095
version: 1
was generated by: iGEM to SBOL conversion
description: CBDcex fused to Phytochelatin (PC) EC20
created: 2014-10-06T11:00:00Z
modified: 2015-06-17T12:34:35Z
mutable provenance: CBD7+PhytoC. TBC
owned by: james
owned by: myers
mutable description: CBD7+PhytoC. TBC
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20780
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: CBD7+PhytoC. TBC
star: false
creator: Xenia Spencer-Milnes
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321003
component: BBa_B0105
component: BBa_K1321005
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321003
sequence annotation: BBa_K1321005
sequence: https://synbiohub.org/public/igem/BBa_K1321095_sequence/1

BBa_K1321003
type: Component
persistent identity: component2414727
display id: component2414727
version: 1
top level: BBa_K1321095
access: public
definition: BBa_K1321003

BBa_B0105
type: Component
persistent identity: component2414729
display id: component2414729
version: 1
top level: BBa_K1321095
access: public
definition: Scar 25

BBa_K1321005
type: Component
persistent identity: component2414731
display id: component2414731
version: 1
top level: BBa_K1321095
access: public
definition: BBa_K1321005

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2414729
display id: annotation2414729
version: 1
top level: BBa_K1321095
location: https://synbiohub.org/public/igem/BBa_K1321095/annotation2414729/range2414729/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321095/annotation2414729/range2414729/1
type: Range
persistent identity: range2414729
display id: range2414729
version: 1
top level: BBa_K1321095
direction: direction/reverse
start: 336
end: 341
orientation: inline

BBa_K1321003
type: SequenceAnnotation
persistent identity: annotation2414727
display id: annotation2414727
version: 1
top level: BBa_K1321095
location: https://synbiohub.org/public/igem/BBa_K1321095/annotation2414727/range2414727/1
component: BBa_K1321003

https://synbiohub.org/public/igem/BBa_K1321095/annotation2414727/range2414727/1
type: Range
persistent identity: range2414727
display id: range2414727
version: 1
top level: BBa_K1321095
direction: direction/reverse
start: 1
end: 327
orientation: inline

BBa_K1321005
type: SequenceAnnotation
persistent identity: annotation2414731
display id: annotation2414731
version: 1
top level: BBa_K1321095
location: https://synbiohub.org/public/igem/BBa_K1321095/annotation2414731/range2414731/1
component: BBa_K1321005

https://synbiohub.org/public/igem/BBa_K1321095/annotation2414731/range2414731/1
type: Range
persistent identity: range2414731
display id: range2414731
version: 1
top level: BBa_K1321095
direction: direction/reverse
start: 350
end: 472
orientation: inline

BBa_K1321351
type: ComponentDefinition
persistent identity: BBa K1321351
display id: BBa_K1321351
version: 1
was generated by: iGEM to SBOL conversion
description: CBDcenA with linker fused to NiBP in Freiburg format (RFC 25)
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:31:45Z
mutable provenance: asdf
owned by: james
owned by: myers
mutable description: asdf
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: asdf
star: false
creator: Chris N Micklem
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_B0105
component: BBa_K1321009
component: BBa_K1321339
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321009
sequence annotation: BBa_K1321339
sequence: https://synbiohub.org/public/igem/BBa_K1321351_sequence/1

BBa_B0105
type: Component
persistent identity: component2430309
display id: component2430309
version: 1
top level: BBa_K1321351
access: public
definition: Scar 25

BBa_K1321009
type: Component
persistent identity: component2430311
display id: component2430311
version: 1
top level: BBa_K1321351
access: public
definition: BBa_K1321009

BBa_K1321339
type: Component
persistent identity: component2430307
display id: component2430307
version: 1
top level: BBa_K1321351
access: public
definition: BBa_K1321339

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2430309
display id: annotation2430309
version: 1
top level: BBa_K1321351
location: https://synbiohub.org/public/igem/BBa_K1321351/annotation2430309/range2430309/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321351/annotation2430309/range2430309/1
type: Range
persistent identity: range2430309
display id: range2430309
version: 1
top level: BBa_K1321351
direction: direction/reverse
start: 409
end: 414
orientation: inline

BBa_K1321009
type: SequenceAnnotation
persistent identity: annotation2430311
display id: annotation2430311
version: 1
top level: BBa_K1321351
location: https://synbiohub.org/public/igem/BBa_K1321351/annotation2430311/range2430311/1
component: BBa_K1321009

https://synbiohub.org/public/igem/BBa_K1321351/annotation2430311/range2430311/1
type: Range
persistent identity: range2430311
display id: range2430311
version: 1
top level: BBa_K1321351
direction: direction/reverse
start: 415
end: 590
orientation: inline

BBa_K1321339
type: SequenceAnnotation
persistent identity: annotation2430307
display id: annotation2430307
version: 1
top level: BBa_K1321351
location: https://synbiohub.org/public/igem/BBa_K1321351/annotation2430307/range2430307/1
component: BBa_K1321339

https://synbiohub.org/public/igem/BBa_K1321351/annotation2430307/range2430307/1
type: Range
persistent identity: range2430307
display id: range2430307
version: 1
top level: BBa_K1321351
direction: direction/reverse
start: 1
end: 408
orientation: inline

CBDclos(T7
type: ComponentDefinition
persistent identity: BBa K863112
display id: BBa_K863112
version: 1
was generated by: iGEM to SBOL conversion
description: Cellulose binding domain of C. cellulovorans with T7, RBS, GS-Linker (Freiburg-Standard)
created: 2012-09-19T11:00:00Z
modified: 2015-06-17T01:20:18Z
mutable provenance: Cloning-vector
owned by: james
owned by: myers
mutable description: Cellulose binding domain of the [http://www.ncbi.nlm.nih.gov/nucleotide/327179207?report=genbank&log$=nucltop&blast_rank=3&RID=152ZCN0E01N Cellulomonas fimi ATCC 484 exoglucanase gene] in Freiburg-standard
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 4206
owner id: 13080
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Added the regulatory unit BBa_K525998 (T7+RBS) in front of the CBD and a short GS-Linker behind the domain and used Freiburg-Standard to be able to fuse proteins to the domain.
star: false
creator: Moritz M??ller
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: B0034
sequence annotation: ATG
sequence annotation: Cellulose Binding Protein
sequence annotation: RBS
sequence annotation: BBa_K525998
sequence annotation: silent mutation (A original)
sequence annotation: GS-Linker
sequence annotation: TAA
sequence annotation: BBa_K863111
sequence annotation: AgeI
sequence annotation: T7 promoter
sequence annotation: Cellulose binding domain (CBDclos)
sequence: https://synbiohub.org/public/igem/BBa_K863112_sequence/1

B0034
type: SequenceAnnotation
persistent identity: annotation2188284
display id: annotation2188284
version: 1
top level: CBDclos(T7
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188284/range2188284/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188284/range2188284/1
type: Range
persistent identity: range2188284
display id: range2188284
version: 1
top level: CBDclos(T7
direction: direction/forward
start: 21
end: 32
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2188287
display id: annotation2188287
version: 1
top level: CBDclos(T7
role: feature/start
role: SO:0000318
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188287/range2188287/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188287/range2188287/1
type: Range
persistent identity: range2188287
display id: range2188287
version: 1
top level: CBDclos(T7
direction: direction/forward
start: 35
end: 38
orientation: inline

Cellulose Binding Protein
type: SequenceAnnotation
persistent identity: annotation2188290
display id: annotation2188290
version: 1
top level: CBDclos(T7
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188290/range2188290/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188290/range2188290/1
type: Range
persistent identity: range2188290
display id: range2188290
version: 1
top level: CBDclos(T7
direction: direction/forward
start: 317
end: 322
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2188285
display id: annotation2188285
version: 1
top level: CBDclos(T7
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188285/range2188285/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188285/range2188285/1
type: Range
persistent identity: range2188285
display id: range2188285
version: 1
top level: CBDclos(T7
direction: direction/forward
start: 24
end: 27
orientation: inline

BBa_K525998
type: SequenceAnnotation
persistent identity: annotation2188283
display id: annotation2188283
version: 1
top level: CBDclos(T7
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188283/range2188283/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188283/range2188283/1
type: Range
persistent identity: range2188283
display id: range2188283
version: 1
top level: CBDclos(T7
direction: direction/forward
start: 1
end: 32
orientation: inline

silent mutation (A original)
type: SequenceAnnotation
persistent identity: annotation2200498
display id: annotation2200498
version: 1
top level: CBDclos(T7
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2200498/range2200498/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2200498/range2200498/1
type: Range
persistent identity: range2200498
display id: range2200498
version: 1
top level: CBDclos(T7
direction: direction/forward
start: 112
end: 112
orientation: inline

GS-Linker
type: SequenceAnnotation
persistent identity: annotation2188291
display id: annotation2188291
version: 1
top level: CBDclos(T7
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188291/range2188291/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188291/range2188291/1
type: Range
persistent identity: range2188291
display id: range2188291
version: 1
top level: CBDclos(T7
direction: direction/forward
start: 323
end: 328
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2188293
display id: annotation2188293
version: 1
top level: CBDclos(T7
role: feature/stop
role: SO:0000319
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188293/range2188293/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188293/range2188293/1
type: Range
persistent identity: range2188293
display id: range2188293
version: 1
top level: CBDclos(T7
direction: direction/forward
start: 335
end: 337
orientation: inline

BBa_K863111
type: SequenceAnnotation
persistent identity: annotation2188286
display id: annotation2188286
version: 1
top level: CBDclos(T7
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188286/range2188286/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188286/range2188286/1
type: Range
persistent identity: range2188286
display id: range2188286
version: 1
top level: CBDclos(T7
direction: direction/forward
start: 35
end: 337
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2188292
display id: annotation2188292
version: 1
top level: CBDclos(T7
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188292/range2188292/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188292/range2188292/1
type: Range
persistent identity: range2188292
display id: range2188292
version: 1
top level: CBDclos(T7
direction: direction/forward
start: 329
end: 334
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2188282
display id: annotation2188282
version: 1
top level: CBDclos(T7
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188282/range2188282/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188282/range2188282/1
type: Range
persistent identity: range2188282
display id: range2188282
version: 1
top level: CBDclos(T7
direction: direction/forward
start: 1
end: 20
orientation: inline

Cellulose binding domain (CBDclos)
type: SequenceAnnotation
persistent identity: annotation2188289
display id: annotation2188289
version: 1
top level: CBDclos(T7
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188289/range2188289/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188289/range2188289/1
type: Range
persistent identity: range2188289
display id: range2188289
version: 1
top level: CBDclos(T7
direction: direction/forward
start: 41
end: 316
orientation: inline

Cellulose Binding Protein
type: SequenceAnnotation
persistent identity: annotation2188288
display id: annotation2188288
version: 1
top level: CBDclos(T7
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188288/range2188288/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188288/range2188288/1
type: Range
persistent identity: range2188288
display id: range2188288
version: 1
top level: CBDclos(T7
direction: direction/forward
start: 35
end: 40
orientation: inline

BBa_K2127002
type: ComponentDefinition
persistent identity: BBa K2127002
display id: BBa_K2127002
version: 1
was generated by: iGEM to SBOL conversion
description: CP47 His-tagged with Lac Promoter
created: 2016-10-13T11:00:00Z
modified: 2016-10-21T07:46:10Z
mutable provenance: NCBI Reference Sequence: NC_000911.1
owned by: james
owned by: myers
mutable description: CP47 His-tagged with Lac Promoter synthesized by IDT DNA Inc.
discontinued: false
dominant: false
group u list: _2596_
m user id: 32415
owner id: 17480
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Double Stop Codon. ORF Check
star: false
creator: Dawson Zeng
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K2127004
component: BBa_K2127007
component: BBa_B0030
component: BBa_R0010
sequence annotation: BBa_K2127007
sequence annotation: BBa_B0030
sequence annotation: BBa_K2127004
sequence annotation: BBa_R0010
sequence: https://synbiohub.org/public/igem/BBa_K2127002_sequence/1

BBa_K2127004
type: Component
persistent identity: component2527113
display id: component2527113
version: 1
top level: BBa_K2127002
access: public
definition: CP47His

BBa_K2127007
type: Component
persistent identity: component2527114
display id: component2527114
version: 1
top level: BBa_K2127002
access: public
definition: MaTif

BBa_B0030
type: Component
persistent identity: component2527107
display id: component2527107
version: 1
top level: BBa_K2127002
access: public
definition: BBa_B0030

BBa_R0010
type: Component
persistent identity: component2527099
display id: component2527099
version: 1
top level: BBa_K2127002
access: public
definition: LacI

BBa_K2127007
type: SequenceAnnotation
persistent identity: annotation2527114
display id: annotation2527114
version: 1
top level: BBa_K2127002
location: https://synbiohub.org/public/igem/BBa_K2127002/annotation2527114/range2527114/1
component: BBa_K2127007

https://synbiohub.org/public/igem/BBa_K2127002/annotation2527114/range2527114/1
type: Range
persistent identity: range2527114
display id: range2527114
version: 1
top level: BBa_K2127002
direction: direction/reverse
start: 1764
end: 1886
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2527107
display id: annotation2527107
version: 1
top level: BBa_K2127002
location: https://synbiohub.org/public/igem/BBa_K2127002/annotation2527107/range2527107/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K2127002/annotation2527107/range2527107/1
type: Range
persistent identity: range2527107
display id: range2527107
version: 1
top level: BBa_K2127002
direction: direction/forward
start: 201
end: 215
orientation: inline

BBa_K2127004
type: SequenceAnnotation
persistent identity: annotation2527113
display id: annotation2527113
version: 1
top level: BBa_K2127002
location: https://synbiohub.org/public/igem/BBa_K2127002/annotation2527113/range2527113/1
component: BBa_K2127004

https://synbiohub.org/public/igem/BBa_K2127002/annotation2527113/range2527113/1
type: Range
persistent identity: range2527113
display id: range2527113
version: 1
top level: BBa_K2127002
direction: direction/reverse
start: 216
end: 1763
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2527099
display id: annotation2527099
version: 1
top level: BBa_K2127002
location: https://synbiohub.org/public/igem/BBa_K2127002/annotation2527099/range2527099/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K2127002/annotation2527099/range2527099/1
type: Range
persistent identity: range2527099
display id: range2527099
version: 1
top level: BBa_K2127002
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_K2127001
type: ComponentDefinition
persistent identity: BBa K2127001
display id: BBa_K2127001
version: 1
was generated by: iGEM to SBOL conversion
description: Inducible High Expression His-Tagged Photosystem II CP47 subunit
created: 2016-10-13T11:00:00Z
modified: 2016-10-21T07:45:18Z
mutable provenance: Synchocisytys
owned by: james
owned by: myers
mutable description: TFBS plac LacO CP47his
discontinued: false
dominant: false
group u list: _2596_
m user id: 0
m user id: 32415
owner id: 32415
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: synthesized
star: false
creator: Nafaa Haddou
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_B0030
component: BBa_K2127006
component: BBa_R0010
component: BBa_K2127004
component: BBa_K2127007
sequence annotation: BBa_B0030
sequence annotation: BBa_K2127006
sequence annotation: BBa_R0010
sequence annotation: BBa_K2127004
sequence annotation: BBa_K2127007
sequence: https://synbiohub.org/public/igem/BBa_K2127001_sequence/1

BBa_B0030
type: Component
persistent identity: component2527075
display id: component2527075
version: 1
top level: BBa_K2127001
access: public
definition: BBa_B0030

BBa_K2127006
type: Component
persistent identity: component2527066
display id: component2527066
version: 1
top level: BBa_K2127001
access: public
definition: TFBS

BBa_R0010
type: Component
persistent identity: component2527067
display id: component2527067
version: 1
top level: BBa_K2127001
access: public
definition: LacI

BBa_K2127004
type: Component
persistent identity: component2527081
display id: component2527081
version: 1
top level: BBa_K2127001
access: public
definition: CP47His

BBa_K2127007
type: Component
persistent identity: component2527082
display id: component2527082
version: 1
top level: BBa_K2127001
access: public
definition: MaTif

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2527075
display id: annotation2527075
version: 1
top level: BBa_K2127001
location: https://synbiohub.org/public/igem/BBa_K2127001/annotation2527075/range2527075/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K2127001/annotation2527075/range2527075/1
type: Range
persistent identity: range2527075
display id: range2527075
version: 1
top level: BBa_K2127001
direction: direction/forward
start: 386
end: 400
orientation: inline

BBa_K2127006
type: SequenceAnnotation
persistent identity: annotation2527066
display id: annotation2527066
version: 1
top level: BBa_K2127001
location: https://synbiohub.org/public/igem/BBa_K2127001/annotation2527066/range2527066/1
component: BBa_K2127006

https://synbiohub.org/public/igem/BBa_K2127001/annotation2527066/range2527066/1
type: Range
persistent identity: range2527066
display id: range2527066
version: 1
top level: BBa_K2127001
direction: direction/reverse
start: 1
end: 185
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2527067
display id: annotation2527067
version: 1
top level: BBa_K2127001
location: https://synbiohub.org/public/igem/BBa_K2127001/annotation2527067/range2527067/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K2127001/annotation2527067/range2527067/1
type: Range
persistent identity: range2527067
display id: range2527067
version: 1
top level: BBa_K2127001
direction: direction/forward
start: 186
end: 385
orientation: inline

BBa_K2127004
type: SequenceAnnotation
persistent identity: annotation2527081
display id: annotation2527081
version: 1
top level: BBa_K2127001
location: https://synbiohub.org/public/igem/BBa_K2127001/annotation2527081/range2527081/1
component: BBa_K2127004

https://synbiohub.org/public/igem/BBa_K2127001/annotation2527081/range2527081/1
type: Range
persistent identity: range2527081
display id: range2527081
version: 1
top level: BBa_K2127001
direction: direction/reverse
start: 401
end: 1948
orientation: inline

BBa_K2127007
type: SequenceAnnotation
persistent identity: annotation2527082
display id: annotation2527082
version: 1
top level: BBa_K2127001
location: https://synbiohub.org/public/igem/BBa_K2127001/annotation2527082/range2527082/1
component: BBa_K2127007

https://synbiohub.org/public/igem/BBa_K2127001/annotation2527082/range2527082/1
type: Range
persistent identity: range2527082
display id: range2527082
version: 1
top level: BBa_K2127001
direction: direction/reverse
start: 1949
end: 2071
orientation: inline

BBa_K1122703
type: ComponentDefinition
persistent identity: BBa K1122703
display id: BBa_K1122703
version: 1
was generated by: iGEM to SBOL conversion
description: Ferric ion-binding protein (FbpA) without a signal peptide
created: 2013-09-21T11:00:00Z
modified: 2015-07-21T11:41:44Z
mutable provenance: ''Neisseria gonorrhoeae'' gDNA
owned by: james
owned by: myers
mutable description: This part encodes a protein capable of binding a ferric ion. The periplasmic signal peptide has been deleted in order to achieve cytoplasmic localisation of the protein.
discontinued: false
dominant: false
experience: experience/None
group u list: _1433_
m user id: 4206
owner id: 18014
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Deletion of a putative native periplasmic signal peptide
star: false
creator: Harry Thornton
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1122703_sequence/1

TFBS
type: ComponentDefinition
persistent identity: BBa K2127006
display id: BBa_K2127006
version: 1
was generated by: iGEM to SBOL conversion
description: Transcription Factor Binding Site from Cyanobacterial Pcpc560
created: 2016-10-13T11:00:00Z
modified: 2016-10-25T10:25:34Z
mutable provenance: Pcpc560-TFBS
owned by: james
owned by: myers
mutable description: Pcpc560-TFBS
discontinued: false
dominant: false
experience: experience/None
group u list: _2596_
m user id: 32415
owner id: 32736
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Pcpc560-TFBS
star: false
creator: Mirat Sojitra
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Transcription Factor Binding Sites
sequence: https://synbiohub.org/public/igem/BBa_K2127006_sequence/1

Transcription Factor Binding Sites
type: SequenceAnnotation
persistent identity: annotation2530242
display id: annotation2530242
version: 1
top level: TFBS
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K2127006/annotation2530242/range2530242/1

https://synbiohub.org/public/igem/BBa_K2127006/annotation2530242/range2530242/1
type: Range
persistent identity: range2530242
display id: range2530242
version: 1
top level: TFBS
direction: direction/forward
start: 1
end: 185
orientation: inline

BBa_K1420001
type: ComponentDefinition
persistent identity: BBa K1420001
display id: BBa_K1420001
version: 1
was generated by: iGEM to SBOL conversion
description: merA, mercuric reductase from Serratia marcescens
created: 2014-09-25T11:00:00Z
modified: 2015-07-22T02:51:48Z
mutable provenance: pDU1358, donated by Anne O. Summers.
owned by: james
owned by: myers
mutable description: Mercuric reductase catalyzes the reduction of Hg to Hg2+.
discontinued: false
dominant: false
group u list: _1798_
m user id: 4206
owner id: 17872
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Mutagenesis of adenine to guanine at position 1026 to remove EcoRI site.
star: false
creator: Stephen C. Heinsch
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: merA
sequence: https://synbiohub.org/public/igem/BBa_K1420001_sequence/1

merA
type: SequenceAnnotation
persistent identity: annotation2387014
display id: annotation2387014
version: 1
top level: BBa_K1420001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1420001/annotation2387014/range2387014/1

https://synbiohub.org/public/igem/BBa_K1420001/annotation2387014/range2387014/1
type: Range
persistent identity: range2387014
display id: range2387014
version: 1
top level: BBa_K1420001
direction: direction/forward
start: 1
end: 1686
orientation: inline

BBa_K1321104
type: ComponentDefinition
persistent identity: BBa K1321104
display id: BBa_K1321104
version: 1
was generated by: iGEM to SBOL conversion
description: CBDclos fused to Phytochelatin (PC) EC20 with T7 promoter
created: 2014-10-08T11:00:00Z
modified: 2015-06-17T12:18:25Z
mutable provenance: CBD4+PhytoC
owned by: james
owned by: myers
mutable description: CBD4+PhytoC
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20780
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: CBD4+PhytoC
star: false
creator: Xenia Spencer-Milnes
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1319106
component: BBa_K1321338
component: BBa_K1321002
component: BBa_K1321005
component: BBa_B0105
sequence annotation: BBa_K1319106
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321005
sequence annotation: BBa_K1321338
sequence annotation: BBa_K1321002
sequence: https://synbiohub.org/public/igem/BBa_K1321104_sequence/1

BBa_K1319106
type: Component
persistent identity: component2420130
display id: component2420130
version: 1
top level: BBa_K1321104
access: public
definition: Scar25 ATG

BBa_K1321338
type: Component
persistent identity: component2420128
display id: component2420128
version: 1
top level: BBa_K1321104
access: public
definition: BBa_K1321338

BBa_K1321002
type: Component
persistent identity: component2420131
display id: component2420131
version: 1
top level: BBa_K1321104
access: public
definition: BBa_K1321002

BBa_K1321005
type: Component
persistent identity: component2420135
display id: component2420135
version: 1
top level: BBa_K1321104
access: public
definition: BBa_K1321005

BBa_B0105
type: Component
persistent identity: component2420133
display id: component2420133
version: 1
top level: BBa_K1321104
access: public
definition: Scar 25

BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2420130
display id: annotation2420130
version: 1
top level: BBa_K1321104
location: https://synbiohub.org/public/igem/BBa_K1321104/annotation2420130/range2420130/1
component: BBa_K1319106

https://synbiohub.org/public/igem/BBa_K1321104/annotation2420130/range2420130/1
type: Range
persistent identity: range2420130
display id: range2420130
version: 1
top level: BBa_K1321104
direction: direction/reverse
start: 44
end: 58
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2420133
display id: annotation2420133
version: 1
top level: BBa_K1321104
location: https://synbiohub.org/public/igem/BBa_K1321104/annotation2420133/range2420133/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321104/annotation2420133/range2420133/1
type: Range
persistent identity: range2420133
display id: range2420133
version: 1
top level: BBa_K1321104
direction: direction/reverse
start: 359
end: 364
orientation: inline

BBa_K1321005
type: SequenceAnnotation
persistent identity: annotation2420135
display id: annotation2420135
version: 1
top level: BBa_K1321104
location: https://synbiohub.org/public/igem/BBa_K1321104/annotation2420135/range2420135/1
component: BBa_K1321005

https://synbiohub.org/public/igem/BBa_K1321104/annotation2420135/range2420135/1
type: Range
persistent identity: range2420135
display id: range2420135
version: 1
top level: BBa_K1321104
direction: direction/reverse
start: 365
end: 487
orientation: inline

BBa_K1321338
type: SequenceAnnotation
persistent identity: annotation2420128
display id: annotation2420128
version: 1
top level: BBa_K1321104
location: https://synbiohub.org/public/igem/BBa_K1321104/annotation2420128/range2420128/1
component: BBa_K1321338

https://synbiohub.org/public/igem/BBa_K1321104/annotation2420128/range2420128/1
type: Range
persistent identity: range2420128
display id: range2420128
version: 1
top level: BBa_K1321104
direction: direction/reverse
start: 1
end: 43
orientation: inline

BBa_K1321002
type: SequenceAnnotation
persistent identity: annotation2420131
display id: annotation2420131
version: 1
top level: BBa_K1321104
location: https://synbiohub.org/public/igem/BBa_K1321104/annotation2420131/range2420131/1
component: BBa_K1321002

https://synbiohub.org/public/igem/BBa_K1321104/annotation2420131/range2420131/1
type: Range
persistent identity: range2420131
display id: range2420131
version: 1
top level: BBa_K1321104
direction: direction/reverse
start: 59
end: 358
orientation: inline

BBa_K1460005
type: ComponentDefinition
persistent identity: BBa K1460005
display id: BBa_K1460005
version: 1
was generated by: iGEM to SBOL conversion
description: Anderson Promoter + RBS + CBP4
created: 2014-08-25T11:00:00Z
modified: 2016-01-13T10:18:44Z
mutable provenance: Anderson promoter is that of part BBa_J23102. CBP4 is a naturally found coding sequence in Nicotiana tabacum.[1] This part was synthesized by BioBasic and was transferred into pSB1C3 using standard cloning procedures.
owned by: james
owned by: myers
mutable description: This is a functional coding sequence for the putative lead and nickel transport protein CBP4 from Nicotiana tabacum. It has been found that expression of this protein leads to increased nickel tolerance and lead intolerance.
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1839_
m user id: 4206
owner id: 15649
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The Anderson promoter will provide strong constitutive expression of the CBP4 gene in E. coli.
star: false
creator: Eric Holmes
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_J61101
sequence annotation: CBP4
sequence annotation: BBa_J23102
sequence: https://synbiohub.org/public/igem/BBa_K1460005_sequence/1

BBa_J61101
type: SequenceAnnotation
persistent identity: annotation2381552
display id: annotation2381552
version: 1
top level: BBa_K1460005
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1460005/annotation2381552/range2381552/1

https://synbiohub.org/public/igem/BBa_K1460005/annotation2381552/range2381552/1
type: Range
persistent identity: range2381552
display id: range2381552
version: 1
top level: BBa_K1460005
direction: direction/forward
start: 43
end: 56
orientation: inline

CBP4
type: SequenceAnnotation
persistent identity: annotation2381553
display id: annotation2381553
version: 1
top level: BBa_K1460005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1460005/annotation2381553/range2381553/1

https://synbiohub.org/public/igem/BBa_K1460005/annotation2381553/range2381553/1
type: Range
persistent identity: range2381553
display id: range2381553
version: 1
top level: BBa_K1460005
direction: direction/forward
start: 63
end: 2189
orientation: inline

BBa_J23102
type: SequenceAnnotation
persistent identity: annotation2381551
display id: annotation2381551
version: 1
top level: BBa_K1460005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1460005/annotation2381551/range2381551/1

https://synbiohub.org/public/igem/BBa_K1460005/annotation2381551/range2381551/1
type: Range
persistent identity: range2381551
display id: range2381551
version: 1
top level: BBa_K1460005
direction: direction/forward
start: 1
end: 35
orientation: inline

BBa_K1321346
type: ComponentDefinition
persistent identity: BBa K1321346
display id: BBa_K1321346
version: 1
was generated by: iGEM to SBOL conversion
description: CBDclos fused to sfGFP in RFC 25
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:30:31Z
mutable provenance: asdf
owned by: james
owned by: myers
mutable description: asdf
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: asdf
star: false
creator: Chris N Micklem
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321002
component: BBa_K1321337
component: BBa_B0105
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321002
sequence annotation: BBa_K1321337
sequence: https://synbiohub.org/public/igem/BBa_K1321346_sequence/1

BBa_K1321002
type: Component
persistent identity: component2415121
display id: component2415121
version: 1
top level: BBa_K1321346
access: public
definition: BBa_K1321002

BBa_K1321337
type: Component
persistent identity: component2415125
display id: component2415125
version: 1
top level: BBa_K1321346
access: public
definition: BBa_K1321337

BBa_B0105
type: Component
persistent identity: component2415123
display id: component2415123
version: 1
top level: BBa_K1321346
access: public
definition: Scar 25

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2415123
display id: annotation2415123
version: 1
top level: BBa_K1321346
location: https://synbiohub.org/public/igem/BBa_K1321346/annotation2415123/range2415123/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321346/annotation2415123/range2415123/1
type: Range
persistent identity: range2415123
display id: range2415123
version: 1
top level: BBa_K1321346
direction: direction/reverse
start: 301
end: 306
orientation: inline

BBa_K1321002
type: SequenceAnnotation
persistent identity: annotation2415121
display id: annotation2415121
version: 1
top level: BBa_K1321346
location: https://synbiohub.org/public/igem/BBa_K1321346/annotation2415121/range2415121/1
component: BBa_K1321002

https://synbiohub.org/public/igem/BBa_K1321346/annotation2415121/range2415121/1
type: Range
persistent identity: range2415121
display id: range2415121
version: 1
top level: BBa_K1321346
direction: direction/reverse
start: 1
end: 300
orientation: inline

BBa_K1321337
type: SequenceAnnotation
persistent identity: annotation2415125
display id: annotation2415125
version: 1
top level: BBa_K1321346
location: https://synbiohub.org/public/igem/BBa_K1321346/annotation2415125/range2415125/1
component: BBa_K1321337

https://synbiohub.org/public/igem/BBa_K1321346/annotation2415125/range2415125/1
type: Range
persistent identity: range2415125
display id: range2415125
version: 1
top level: BBa_K1321346
direction: direction/reverse
start: 307
end: 1017
orientation: inline

BBa_K1438002
type: ComponentDefinition
persistent identity: BBa K1438002
display id: BBa_K1438002
version: 1
was generated by: iGEM to SBOL conversion
description: Hu_Ferritin
created: 2014-10-05T11:00:00Z
modified: 2015-07-22T02:13:57Z
mutable provenance: iGEM Calgary 2013 from a synthesized codon optimized construct
owned by: james
owned by: myers
mutable description: Human ferritins do loads more iron atoms then bacterial ferritins. Furthermore they are forming metall cores that are less contaminated with phosphates then e.g. bacterial ferritins. Due to this fact, this should result in a purer iron crystal with an improved magnetic moment.
discontinued: false
dominant: false
group u list: _1816_
m user id: 4206
owner id: 19714
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This design was provided by iGEM Calgary and the HuFerritin as a single protein coding biobrick was constructed by iGEM Berlin as this biobrick was not accessible in this version in the registry.
star: false
creator: Johann Bauerfeind
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1438002_sequence/1

BBa_K1499004
type: ComponentDefinition
persistent identity: BBa K1499004
display id: BBa_K1499004
version: 1
was generated by: iGEM to SBOL conversion
description: Cellulose binding domains with streptavidin domain generator
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:53:51Z
mutable provenance: organism 1 organism 2
owned by: james
owned by: myers
mutable description: todo
discontinued: false
dominant: false
group u list: _1879_
m user id: 4206
owner id: 11095
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Different sequences for cellulose-binding domains to prevent homologous recombination. Removed cut sites for RFC-10 compatibility.
star: false
creator: Raman Nelakanti
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: Cellulose-binding(clostridium cellulovorans)
sequence annotation: Streptavidin
sequence: https://synbiohub.org/public/igem/BBa_K1499004_sequence/1

Cellulose-binding(clostridium cellulovorans)
type: SequenceAnnotation
persistent identity: annotation2429345
display id: annotation2429345
version: 1
top level: BBa_K1499004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1499004/annotation2429345/range2429345/1

https://synbiohub.org/public/igem/BBa_K1499004/annotation2429345/range2429345/1
type: Range
persistent identity: range2429345
display id: range2429345
version: 1
top level: BBa_K1499004
direction: direction/forward
start: 55
end: 352
orientation: inline

Streptavidin
type: SequenceAnnotation
persistent identity: annotation2429346
display id: annotation2429346
version: 1
top level: BBa_K1499004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1499004/annotation2429346/range2429346/1

https://synbiohub.org/public/igem/BBa_K1499004/annotation2429346/range2429346/1
type: Range
persistent identity: range2429346
display id: range2429346
version: 1
top level: BBa_K1499004
direction: direction/forward
start: 373
end: 750
orientation: inline

BBa_K1122702
type: ComponentDefinition
persistent identity: BBa K1122702
display id: BBa_K1122702
version: 1
was generated by: iGEM to SBOL conversion
description: Ferric ion-binding protein (FbpA)
created: 2013-09-21T11:00:00Z
modified: 2015-07-21T11:42:01Z
mutable provenance: ''Neisseria gonorrhoeae'' gDNA
owned by: james
owned by: myers
mutable description: This parts encodes a protein capable of binding ferric ion. The protein includes a signal peptide targeting it to periplasmic space.
discontinued: false
dominant: false
experience: experience/None
group u list: _1433_
m user id: 4206
owner id: 18014
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: none
star: false
creator: Harry Thornton
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1122702_sequence/1

pT7
type: ComponentDefinition
persistent identity: BBa I719005
display id: BBa_I719005
version: 1
was generated by: iGEM to SBOL conversion
description: T7 Promoter
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:07:53Z
mutable provenance: ---
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Just a T7 Promoter
discontinued: false
dominant: false
experience: experience/Works
group u list: _128_
m user id: 0
owner id: 2097
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: None
star: true
creator: Imperial 2007
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I719005_sequence/1

BBa_K643000
type: ComponentDefinition
persistent identity: BBa K643000
display id: BBa_K643000
version: 1
was generated by: iGEM to SBOL conversion
description: CDS7 cadmium sulfate binding peptide coding sequence
created: 2011-09-24T11:00:00Z
modified: 2015-07-21T02:29:26Z
mutable provenance: This part was synthesized de novo based on the information in the original paper. It was PCR'd out of the synthesized plasmid with RFC 23 overhangs and ligated into pSB1C3 vector.
owned by: james
owned by: myers
mutable description: This sequence codes for a short peptide that's been reported to bind to cadmium sulfate and nucleate quantum dot formation. (C. Mi et al. / Journal of Biotechnology 153 (2011) 125???132) Peptide sequence: N-GDVHHHGRHGAEHADI-C
discontinued: false
dominant: false
experience: experience/Issues
group u list: _819_
m user id: 4206
owner id: 6460
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Original coding sequence in the referenced paper had NcoI and BamHI sites surrounding the coding sequence for cloning into pET28b IPTG inducible vector. For our purposes we eliminated the restriction sites and instead replaced them with standard biobricking prefix and suffix.
star: false
creator: Sung won Lim
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K643000_sequence/1

BBa_K1321014
type: ComponentDefinition
persistent identity: BBa K1321014
display id: BBa_K1321014
version: 1
was generated by: iGEM to SBOL conversion
description: CBDCipA with N and C-terminal linker
created: 2014-10-14T11:00:00Z
modified: 2015-06-17T12:14:33Z
mutable provenance: The designed construct was ordered from as a GeneArt?? String (Invitrogen??? Life Technologies).
owned by: james
owned by: myers
mutable description: This CBD is from the Cellulosomal -scaffolding protein A (cipA) of Clostridium thermocellum including the endogenous linker sequences at the N and C-terminus (UniProt ID Q06851 link http://www.uniprot.org/uniprot/Q06851). It is in RFC25 format to allow for easy use in protein fusions. The CBD is part of the CBM3 family (link to cazy http://www.cazy.org/CBM3.html). This CBD has been used in many application: in fusions with cell adhesion peptides to enhance the properties of cellulose as a cell-growth matrix (Andrade et al 2010a, Andrade et al 2010b), fused to enzymes to remove contaminants from water (Kauffmann et al 2000) and fused to an antimicrobial peptide (Ramos, Domingues & Gama 2010). At present the cloning for these constructs is still in progress to correct an illegal EcoRI site which was identified in the parts with this CBD. This can be achieved with a silent mutation via site-directed mutagenesis and we aim to send these parts to the registry once this is complete.
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20780
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: The DNA sequence from the relevant portion of http://www.ncbi.nlm.nih.gov/nuccore/144777 was codon optimised for ''E.coli''. RFC[25] prefix and suffix were appended to the sequence with an additional 4 basepairs (gatc) at the beginning and end to allow space for the restriction enzymes to bind to the EcoRI and PstI sites at the ends of the sequence for cloning purposes.
star: false
creator: Xenia Spencer-Milnes
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: CBDcipA
sequence annotation: Endogenous Linker (C-term)
sequence annotation: Endogenous Linker (N-term)
sequence: https://synbiohub.org/public/igem/BBa_K1321014_sequence/1

CBDcipA
type: SequenceAnnotation
persistent identity: annotation2420186
display id: annotation2420186
version: 1
top level: BBa_K1321014
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1321014/annotation2420186/range2420186/1

https://synbiohub.org/public/igem/BBa_K1321014/annotation2420186/range2420186/1
type: Range
persistent identity: range2420186
display id: range2420186
version: 1
top level: BBa_K1321014
direction: direction/forward
start: 127
end: 603
orientation: inline

Endogenous Linker (C-term)
type: SequenceAnnotation
persistent identity: annotation2420187
display id: annotation2420187
version: 1
top level: BBa_K1321014
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1321014/annotation2420187/range2420187/1

https://synbiohub.org/public/igem/BBa_K1321014/annotation2420187/range2420187/1
type: Range
persistent identity: range2420187
display id: range2420187
version: 1
top level: BBa_K1321014
direction: direction/forward
start: 604
end: 711
orientation: inline

Endogenous Linker (N-term)
type: SequenceAnnotation
persistent identity: annotation2420141
display id: annotation2420141
version: 1
top level: BBa_K1321014
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1321014/annotation2420141/range2420141/1

https://synbiohub.org/public/igem/BBa_K1321014/annotation2420141/range2420141/1
type: Range
persistent identity: range2420141
display id: range2420141
version: 1
top level: BBa_K1321014
direction: direction/forward
start: 1
end: 126
orientation: inline

BBa_K1321090
type: ComponentDefinition
persistent identity: BBa K1321090
display id: BBa_K1321090
version: 1
was generated by: iGEM to SBOL conversion
description: Phytochelatin (PC) EC20 + Linker-dCBD
created: 2014-10-06T11:00:00Z
modified: 2015-06-17T12:17:05Z
mutable provenance: CBD2 is EnLinker-CBH2-EnLinker-CBH1 from T.reesei. TBC
owned by: james
owned by: myers
mutable description: Phytochelatin EC(20) +CBD2. TBC
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20780
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Codon optimised for E.coli. TBC
star: false
creator: Xenia Spencer-Milnes
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_B0105
component: BBa_K1321340
component: BBa_K1321005
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321340
sequence annotation: BBa_K1321005
sequence: https://synbiohub.org/public/igem/BBa_K1321090_sequence/1

BBa_B0105
type: Component
persistent identity: component2414671
display id: component2414671
version: 1
top level: BBa_K1321090
access: public
definition: Scar 25

BBa_K1321340
type: Component
persistent identity: component2414673
display id: component2414673
version: 1
top level: BBa_K1321090
access: public
definition: BBa_K1321340

BBa_K1321005
type: Component
persistent identity: component2414669
display id: component2414669
version: 1
top level: BBa_K1321090
access: public
definition: BBa_K1321005

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2414671
display id: annotation2414671
version: 1
top level: BBa_K1321090
location: https://synbiohub.org/public/igem/BBa_K1321090/annotation2414671/range2414671/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321090/annotation2414671/range2414671/1
type: Range
persistent identity: range2414671
display id: range2414671
version: 1
top level: BBa_K1321090
direction: direction/reverse
start: 124
end: 129
orientation: inline

BBa_K1321340
type: SequenceAnnotation
persistent identity: annotation2414673
display id: annotation2414673
version: 1
top level: BBa_K1321090
location: https://synbiohub.org/public/igem/BBa_K1321090/annotation2414673/range2414673/1
component: BBa_K1321340

https://synbiohub.org/public/igem/BBa_K1321090/annotation2414673/range2414673/1
type: Range
persistent identity: range2414673
display id: range2414673
version: 1
top level: BBa_K1321090
direction: direction/reverse
start: 130
end: 495
orientation: inline

BBa_K1321005
type: SequenceAnnotation
persistent identity: annotation2414669
display id: annotation2414669
version: 1
top level: BBa_K1321090
location: https://synbiohub.org/public/igem/BBa_K1321090/annotation2414669/range2414669/1
component: BBa_K1321005

https://synbiohub.org/public/igem/BBa_K1321090/annotation2414669/range2414669/1
type: Range
persistent identity: range2414669
display id: range2414669
version: 1
top level: BBa_K1321090
direction: direction/reverse
start: 1
end: 123
orientation: inline

BBa_K1980011
type: ComponentDefinition
persistent identity: BBa K1980011
display id: BBa_K1980011
version: 1
was generated by: iGEM to SBOL conversion
description: pCopA MymT with divergent expressed CueR
created: 2016-10-10T11:00:00Z
modified: 2016-10-24T03:08:06Z
mutable provenance: E. coli TB
owned by: james
owned by: myers
mutable description: pCopA MymT with plasmid constitutively expressed CueR
discontinued: false
dominant: false
experience: experience/Issues
group u list: _2447_
m user id: 29607
owner id: 29607
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: stuff
star: false
creator: Sam Garforth
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K1980006
component: BBa_K1980002
sequence annotation: BBa_K1980006
sequence annotation: BBa_K1980002
sequence: https://synbiohub.org/public/igem/BBa_K1980011_sequence/1

BBa_K1980006
type: Component
persistent identity: component2493043
display id: component2493043
version: 1
top level: BBa_K1980011
access: public
definition: BBa_K1980006

BBa_K1980002
type: Component
persistent identity: component2493044
display id: component2493044
version: 1
top level: BBa_K1980011
access: public
definition: MymT

BBa_K1980006
type: SequenceAnnotation
persistent identity: annotation2493043
display id: annotation2493043
version: 1
top level: BBa_K1980011
location: https://synbiohub.org/public/igem/BBa_K1980011/annotation2493043/range2493043/1
component: BBa_K1980006

https://synbiohub.org/public/igem/BBa_K1980011/annotation2493043/range2493043/1
type: Range
persistent identity: range2493043
display id: range2493043
version: 1
top level: BBa_K1980011
direction: direction/reverse
start: 1
end: 574
orientation: inline

BBa_K1980002
type: SequenceAnnotation
persistent identity: annotation2493044
display id: annotation2493044
version: 1
top level: BBa_K1980011
location: https://synbiohub.org/public/igem/BBa_K1980011/annotation2493044/range2493044/1
component: BBa_K1980002

https://synbiohub.org/public/igem/BBa_K1980011/annotation2493044/range2493044/1
type: Range
persistent identity: range2493044
display id: range2493044
version: 1
top level: BBa_K1980011
direction: direction/reverse
start: 581
end: 763
orientation: inline

BBa_K1694006
type: ComponentDefinition
persistent identity: BBa K1694006
display id: BBa_K1694006
version: 1
was generated by: iGEM to SBOL conversion
description: Gold Binding Polypeptide
created: 2015-09-14T11:00:00Z
modified: 2016-01-25T01:09:40Z
mutable provenance: Artificial synthesis
owned by: james
owned by: myers
mutable description: Gold binding peptides (GBP) is a kind of peptide which can bind on gold, usually used to immobilize protein on gold surface.
discontinued: false
dominant: false
experience: experience/Works
group u list: _2112_
m user id: 4206
owner id: 28523
owning group id: 9
status: status/Planning
bookmark: true
mutable notes: No
star: false
creator: CHIH-HSUAN HSU
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1694006_sequence/1

BBa_K519011
type: ComponentDefinition
persistent identity: BBa K519011
display id: BBa_K519011
version: 1
was generated by: iGEM to SBOL conversion
description: SmtA-Double Term.
created: 2011-09-29T11:00:00Z
modified: 2016-01-13T12:59:12Z
mutable provenance: Synechococcus sp. PCC7942
owned by: james
owned by: myers
mutable description: SmtA is a metallothionein that can bind to metal ions such as Cd(II).
discontinued: false
dominant: true
experience: experience/None
group u list: _684_
m user id: 4206
owner id: 9603
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: This part is put together with Double terminator.
star: false
creator: Kotone Miyake
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K519010
component: BBa_B0015
sequence annotation: BBa_K519010
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K519011_sequence/1

BBa_K519010
type: Component
persistent identity: component2147679
display id: component2147679
version: 1
top level: BBa_K519011
access: public
definition: BBa_K519010

BBa_B0015
type: Component
persistent identity: component2147686
display id: component2147686
version: 1
top level: BBa_K519011
access: public
definition: BBa_B0015

BBa_K519010
type: SequenceAnnotation
persistent identity: annotation2147679
display id: annotation2147679
version: 1
top level: BBa_K519011
location: https://synbiohub.org/public/igem/BBa_K519011/annotation2147679/range2147679/1
component: BBa_K519010

https://synbiohub.org/public/igem/BBa_K519011/annotation2147679/range2147679/1
type: Range
persistent identity: range2147679
display id: range2147679
version: 1
top level: BBa_K519011
direction: direction/reverse
start: 1
end: 171
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2147686
display id: annotation2147686
version: 1
top level: BBa_K519011
location: https://synbiohub.org/public/igem/BBa_K519011/annotation2147686/range2147686/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K519011/annotation2147686/range2147686/1
type: Range
persistent identity: range2147686
display id: range2147686
version: 1
top level: BBa_K519011
direction: direction/reverse
start: 180
end: 308
orientation: inline

BBa_K160001
type: ComponentDefinition
persistent identity: BBa K160001
display id: BBa_K160001
version: 1
was generated by: iGEM to SBOL conversion
description: Fe-Receptor
created: 2008-10-24T11:00:00Z
modified: 2015-07-20T02:34:02Z
mutable provenance: E.coli genome
owned by: james
owned by: myers
mutable description: Iron binding protein
discontinued: false
dominant: false
group u list: _238_
m user id: 4206
owner id: 2946
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: N/A
star: false
creator: RAUL CUERO
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K160001_sequence/1

BBa_K1321105
type: ComponentDefinition
persistent identity: BBa K1321105
display id: BBa_K1321105
version: 1
was generated by: iGEM to SBOL conversion
description: CBDcex fused to Phytochelatin (PC) EC20 with T7 promoter
created: 2014-10-08T11:00:00Z
modified: 2015-06-17T12:18:49Z
mutable provenance: CBD7+PhytoC
owned by: james
owned by: myers
mutable description: CBD7+PhytoC
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20780
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: CBD7+PhytoC
star: false
creator: Xenia Spencer-Milnes
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_B0105
component: BBa_K1319106
component: BBa_K1321338
component: BBa_K1321003
component: BBa_K1321005
sequence annotation: BBa_K1321005
sequence annotation: BBa_K1321338
sequence annotation: BBa_K1319106
sequence annotation: BBa_K1321003
sequence annotation: BBa_B0105
sequence: https://synbiohub.org/public/igem/BBa_K1321105_sequence/1

BBa_B0105
type: Component
persistent identity: component2415332
display id: component2415332
version: 1
top level: BBa_K1321105
access: public
definition: Scar 25

BBa_K1319106
type: Component
persistent identity: component2415329
display id: component2415329
version: 1
top level: BBa_K1321105
access: public
definition: Scar25 ATG

BBa_K1321338
type: Component
persistent identity: component2415327
display id: component2415327
version: 1
top level: BBa_K1321105
access: public
definition: BBa_K1321338

BBa_K1321003
type: Component
persistent identity: component2415330
display id: component2415330
version: 1
top level: BBa_K1321105
access: public
definition: BBa_K1321003

BBa_K1321005
type: Component
persistent identity: component2415334
display id: component2415334
version: 1
top level: BBa_K1321105
access: public
definition: BBa_K1321005

BBa_K1321005
type: SequenceAnnotation
persistent identity: annotation2415334
display id: annotation2415334
version: 1
top level: BBa_K1321105
location: https://synbiohub.org/public/igem/BBa_K1321105/annotation2415334/range2415334/1
component: BBa_K1321005

https://synbiohub.org/public/igem/BBa_K1321105/annotation2415334/range2415334/1
type: Range
persistent identity: range2415334
display id: range2415334
version: 1
top level: BBa_K1321105
direction: direction/reverse
start: 392
end: 514
orientation: inline

BBa_K1321338
type: SequenceAnnotation
persistent identity: annotation2415327
display id: annotation2415327
version: 1
top level: BBa_K1321105
location: https://synbiohub.org/public/igem/BBa_K1321105/annotation2415327/range2415327/1
component: BBa_K1321338

https://synbiohub.org/public/igem/BBa_K1321105/annotation2415327/range2415327/1
type: Range
persistent identity: range2415327
display id: range2415327
version: 1
top level: BBa_K1321105
direction: direction/reverse
start: 1
end: 43
orientation: inline

BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2415329
display id: annotation2415329
version: 1
top level: BBa_K1321105
location: https://synbiohub.org/public/igem/BBa_K1321105/annotation2415329/range2415329/1
component: BBa_K1319106

https://synbiohub.org/public/igem/BBa_K1321105/annotation2415329/range2415329/1
type: Range
persistent identity: range2415329
display id: range2415329
version: 1
top level: BBa_K1321105
direction: direction/reverse
start: 44
end: 58
orientation: inline

BBa_K1321003
type: SequenceAnnotation
persistent identity: annotation2415330
display id: annotation2415330
version: 1
top level: BBa_K1321105
location: https://synbiohub.org/public/igem/BBa_K1321105/annotation2415330/range2415330/1
component: BBa_K1321003

https://synbiohub.org/public/igem/BBa_K1321105/annotation2415330/range2415330/1
type: Range
persistent identity: range2415330
display id: range2415330
version: 1
top level: BBa_K1321105
direction: direction/reverse
start: 59
end: 385
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2415332
display id: annotation2415332
version: 1
top level: BBa_K1321105
location: https://synbiohub.org/public/igem/BBa_K1321105/annotation2415332/range2415332/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321105/annotation2415332/range2415332/1
type: Range
persistent identity: range2415332
display id: range2415332
version: 1
top level: BBa_K1321105
direction: direction/reverse
start: 386
end: 391
orientation: inline

BBa_K1321009
type: ComponentDefinition
persistent identity: BBa K1321009
display id: BBa_K1321009
version: 1
was generated by: iGEM to SBOL conversion
description: Nickel Binding Protein (NiBP) Freiburg format (RFC[25])
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:09:51Z
mutable provenance: This part is based on the existing BioBrick BBa_K1151001 by the 2013 Salente Lecce iGEM team.
owned by: james
owned by: myers
mutable description: Nickel Binding Protein in Freiburg format (RFC 25) to allow for easy use in fusion proteins.
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 0
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: OEPCR primers were designed to allow for the replacement of the standard BioBrick prefix and suffix with the Freiburg prefix and suffix.
star: false
creator: Chris N Micklem
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Nickel Binding Protein
sequence: https://synbiohub.org/public/igem/BBa_K1321009_sequence/1

Nickel Binding Protein
type: SequenceAnnotation
persistent identity: annotation2416941
display id: annotation2416941
version: 1
top level: BBa_K1321009
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1321009/annotation2416941/range2416941/1

https://synbiohub.org/public/igem/BBa_K1321009/annotation2416941/range2416941/1
type: Range
persistent identity: range2416941
display id: range2416941
version: 1
top level: BBa_K1321009
direction: direction/forward
start: 1
end: 176
orientation: inline

BBa_K190021
type: ComponentDefinition
persistent identity: BBa K190021
display id: BBa_K190021
version: 1
was generated by: iGEM to SBOL conversion
description: SmtA
created: 2009-08-18T11:00:00Z
modified: 2016-01-13T12:54:44Z
mutable provenance: The gene was cloned from pET29a-SmtA (kindly provided by Kevin Waldron, Newcastle University), it's origin is from the genome of ''Synechococcus PC''
owned by: james
owned by: myers
mutable description: SmtA is a metallothionein that binds Zn (II) ions, it is able to bind 3-4 ions per protein.
discontinued: false
dominant: false
experience: experience/None
group u list: _306_
m user id: 4206
owner id: 4147
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The BioBrick prefix and suffix were added by PCR, also the RBS (B0034) was added.
star: false
creator: Nienke Kuipers
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: B0034
sequence annotation: SmtA
sequence: https://synbiohub.org/public/igem/BBa_K190021_sequence/1

B0034
type: SequenceAnnotation
persistent identity: annotation2017544
display id: annotation2017544
version: 1
top level: BBa_K190021
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K190021/annotation2017544/range2017544/1

https://synbiohub.org/public/igem/BBa_K190021/annotation2017544/range2017544/1
type: Range
persistent identity: range2017544
display id: range2017544
version: 1
top level: BBa_K190021
direction: direction/forward
start: 1
end: 18
orientation: inline

SmtA
type: SequenceAnnotation
persistent identity: annotation2017545
display id: annotation2017545
version: 1
top level: BBa_K190021
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K190021/annotation2017545/range2017545/1

https://synbiohub.org/public/igem/BBa_K190021/annotation2017545/range2017545/1
type: Range
persistent identity: range2017545
display id: range2017545
version: 1
top level: BBa_K190021
direction: direction/forward
start: 19
end: 189
orientation: inline

BBa_K1321094
type: ComponentDefinition
persistent identity: BBa K1321094
display id: BBa_K1321094
version: 1
was generated by: iGEM to SBOL conversion
description: CBDclos fused to Phytochelatin (PC) EC20
created: 2014-10-06T11:00:00Z
modified: 2015-06-17T12:17:50Z
mutable provenance: CBD4+PhytoC. TBC
owned by: james
owned by: myers
mutable description: CBD4+PhytoC. TBC
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20780
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: CBD4+PhytoC. TBC
star: false
creator: Xenia Spencer-Milnes
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_B0105
component: BBa_K1321005
component: BBa_K1321002
sequence annotation: BBa_K1321002
sequence annotation: BBa_K1321005
sequence annotation: BBa_B0105
sequence: https://synbiohub.org/public/igem/BBa_K1321094_sequence/1

BBa_B0105
type: Component
persistent identity: component2414633
display id: component2414633
version: 1
top level: BBa_K1321094
access: public
definition: Scar 25

BBa_K1321005
type: Component
persistent identity: component2414635
display id: component2414635
version: 1
top level: BBa_K1321094
access: public
definition: BBa_K1321005

BBa_K1321002
type: Component
persistent identity: component2414631
display id: component2414631
version: 1
top level: BBa_K1321094
access: public
definition: BBa_K1321002

BBa_K1321002
type: SequenceAnnotation
persistent identity: annotation2414631
display id: annotation2414631
version: 1
top level: BBa_K1321094
location: https://synbiohub.org/public/igem/BBa_K1321094/annotation2414631/range2414631/1
component: BBa_K1321002

https://synbiohub.org/public/igem/BBa_K1321094/annotation2414631/range2414631/1
type: Range
persistent identity: range2414631
display id: range2414631
version: 1
top level: BBa_K1321094
direction: direction/reverse
start: 1
end: 300
orientation: inline

BBa_K1321005
type: SequenceAnnotation
persistent identity: annotation2414635
display id: annotation2414635
version: 1
top level: BBa_K1321094
location: https://synbiohub.org/public/igem/BBa_K1321094/annotation2414635/range2414635/1
component: BBa_K1321005

https://synbiohub.org/public/igem/BBa_K1321094/annotation2414635/range2414635/1
type: Range
persistent identity: range2414635
display id: range2414635
version: 1
top level: BBa_K1321094
direction: direction/reverse
start: 307
end: 429
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2414633
display id: annotation2414633
version: 1
top level: BBa_K1321094
location: https://synbiohub.org/public/igem/BBa_K1321094/annotation2414633/range2414633/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321094/annotation2414633/range2414633/1
type: Range
persistent identity: range2414633
display id: range2414633
version: 1
top level: BBa_K1321094
direction: direction/reverse
start: 301
end: 306
orientation: inline

BBa_K1471000
type: ComponentDefinition
persistent identity: BBa K1471000
display id: BBa_K1471000
version: 1
was generated by: iGEM to SBOL conversion
description: MerE.
created: 2014-10-07T11:00:00Z
modified: 2015-07-22T02:39:55Z
mutable provenance: Bacterial operon mer.
owned by: james
owned by: myers
mutable description: For mercury bioaccumulation.
discontinued: false
dominant: false
experience: experience/None
group u list: _1850_
m user id: 4206
owner id: 16464
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: We have to optimizes its codons in Arabidopsis Thaliana and removed the restriction sites for EcoR1, Xba1, Spe1 and Pst1.
star: false
creator: Juan No?? Hern??ndez Salazar
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1471000_sequence/1

Scar25 ATG
type: ComponentDefinition
persistent identity: BBa K1319106
display id: BBa_K1319106
version: 1
was generated by: iGEM to SBOL conversion
description: RFC 25 Translation Start Scar Sequence
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:09:51Z
mutable provenance: This sequence is produced by an assembly method.
owned by: james
owned by: myers
mutable description: This sequence is produced by RFC10-assembly of a RFC10-RBS with a RFC25-prefixed part.
discontinued: false
dominant: false
experience: experience/None
group u list: _1694_
m user id: 0
owner id: 19522
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The resulting sequence is a combination of the RFC10 RBS-CDS scar (tactag) and 9 nucleotides from the RFC25 prefix (atg gcc ggc).
star: false
creator: Michael Osthege
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K1319106_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2393317
display id: annotation2393317
version: 1
top level: Scar25 ATG
role: feature/start
role: SO:0000318
location: https://synbiohub.org/public/igem/BBa_K1319106/annotation2393317/range2393317/1

https://synbiohub.org/public/igem/BBa_K1319106/annotation2393317/range2393317/1
type: Range
persistent identity: range2393317
display id: range2393317
version: 1
top level: Scar25 ATG
direction: direction/forward
start: 7
end: 9
orientation: inline

BBa_K1321012
type: ComponentDefinition
persistent identity: BBa K1321012
display id: BBa_K1321012
version: 1
was generated by: iGEM to SBOL conversion
description: fMT metallothionein in Freiburg format (RFC[25])
created: 2014-10-09T11:00:00Z
modified: 2015-05-08T01:09:51Z
mutable provenance: From part BBa___ except codon optimised
owned by: james
owned by: myers
mutable description: fMT metallothionein in rfc25 format. This part is based on BBa_____. It can be found as fusions in the following parts:
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 0
owner id: 20780
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: From part BBa___ except codon optimised
star: false
creator: Xenia Spencer-Milnes
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: fMT
sequence: https://synbiohub.org/public/igem/BBa_K1321012_sequence/1

fMT
type: SequenceAnnotation
persistent identity: annotation2414611
display id: annotation2414611
version: 1
top level: BBa_K1321012
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1321012/annotation2414611/range2414611/1

https://synbiohub.org/public/igem/BBa_K1321012/annotation2414611/range2414611/1
type: Range
persistent identity: range2414611
display id: range2414611
version: 1
top level: BBa_K1321012
direction: direction/forward
start: 1
end: 198
orientation: inline

BBa_K1526007
type: ComponentDefinition
persistent identity: BBa K1526007
display id: BBa_K1526007
version: 1
was generated by: iGEM to SBOL conversion
description: LacRS + MntH
created: 2014-10-06T11:00:00Z
modified: 2015-07-24T12:42:53Z
mutable provenance: lacI + NRAMP
owned by: james
owned by: myers
mutable description: lacI + NRAMP
discontinued: false
dominant: false
experience: experience/None
group u list: _1908_
m user id: 4206
owner id: 12384
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: lacI + NRAMP
star: false
creator: Cau?? Westmann
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: LacRS
sequence: https://synbiohub.org/public/igem/BBa_K1526007_sequence/1

LacRS
type: SequenceAnnotation
persistent identity: annotation2427896
display id: annotation2427896
version: 1
top level: BBa_K1526007
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1526007/annotation2427896/range2427896/1

https://synbiohub.org/public/igem/BBa_K1526007/annotation2427896/range2427896/1
type: Range
persistent identity: range2427896
display id: range2427896
version: 1
top level: BBa_K1526007
direction: direction/forward
start: 1
end: 1558
orientation: inline

BBa_K346003
type: ComponentDefinition
persistent identity: BBa K346003
display id: BBa_K346003
version: 1
was generated by: iGEM to SBOL conversion
description: RBS(B0032)+MBP(mercury metal binding peptide engineered from MerR)
created: 2010-10-13T11:00:00Z
modified: 2015-07-21T02:51:54Z
mutable provenance: Tn
owned by: james
owned by: myers
mutable description: This part is a combination of a rbs, a mbp, coding a mercury metal binding peptide egineered from MerR, and a terminator.
discontinued: false
dominant: false
experience: experience/Works
group u list: _457_
m user id: 4206
owner id: 6045
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: no
star: false
creator: Huyang Tengxin
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: conserved
sequence annotation: BBa_B0032
sequence annotation: mbp(mercury binding peptide)
sequence: https://synbiohub.org/public/igem/BBa_K346003_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation2090101
display id: annotation2090101
version: 1
top level: BBa_K346003
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K346003/annotation2090101/range2090101/1

https://synbiohub.org/public/igem/BBa_K346003/annotation2090101/range2090101/1
type: Range
persistent identity: range2090101
display id: range2090101
version: 1
top level: BBa_K346003
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2090100
display id: annotation2090100
version: 1
top level: BBa_K346003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K346003/annotation2090100/range2090100/1

https://synbiohub.org/public/igem/BBa_K346003/annotation2090100/range2090100/1
type: Range
persistent identity: range2090100
display id: range2090100
version: 1
top level: BBa_K346003
direction: direction/forward
start: 1
end: 13
orientation: inline

mbp(mercury binding peptide)
type: SequenceAnnotation
persistent identity: annotation2090102
display id: annotation2090102
version: 1
top level: BBa_K346003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K346003/annotation2090102/range2090102/1

https://synbiohub.org/public/igem/BBa_K346003/annotation2090102/range2090102/1
type: Range
persistent identity: range2090102
display id: range2090102
version: 1
top level: BBa_K346003
direction: direction/forward
start: 19
end: 342
orientation: inline

BBa_K1321003
type: ComponentDefinition
persistent identity: BBa K1321003
display id: BBa_K1321003
version: 1
was generated by: iGEM to SBOL conversion
description: Re-entry of BBa_K863101, Cellulose Binding Domain CBDcex in rfc25
created: 2014-10-09T11:00:00Z
modified: 2015-06-17T12:35:33Z
mutable provenance: This is a direct copy of BBa_K863101 with slight modifications to the beginning and end of the sequence for the purposes of allowing composite parts to be added correctly using this part.
owned by: james
owned by: myers
mutable description: This part is a copy of BBa_K863101 (Cellulose binding Domain of Cellulomonas Fimi Exoglucanse (Freiburg-Standard)) and was created only as a registry entry, removing the atg-NgoMIV parts at the front and the AgeI-taa basepairs from the sequence so that the registry does not flag it as rfc25 incompatible, and it enables us to enter fusion proteins with this part as automatic composite parts in the method as described by: http://2009.igem.org/Team:Freiburg_bioware/general.
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
owner id: 20780
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This is a direct copy of BBa_K863101 with slight modifications to the beginning and end of the sequence for the purposes of allowing composite parts to be added correctly using this part.
star: false
creator: Xenia Spencer-Milnes
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1321003_sequence/1

BBa_K1321356
type: ComponentDefinition
persistent identity: BBa K1321356
display id: BBa_K1321356
version: 1
was generated by: iGEM to SBOL conversion
description: sfGFP fused to CBDclos driven by LacI
created: 2014-10-07T11:00:00Z
modified: 2016-01-26T09:45:38Z
mutable provenance: asdf
owned by: myers
owned by: james
mutable description: asdf
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: awef
star: false
creator: Chris N Micklem
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1319106
component: BBa_K1321341
component: BBa_J04500
sequence annotation: BBa_K1321341
sequence annotation: BBa_J04500
sequence annotation: BBa_K1319106
sequence: https://synbiohub.org/public/igem/BBa_K1321356_sequence/1

BBa_K1319106
type: Component
persistent identity: component2415483
display id: component2415483
version: 1
top level: BBa_K1321356
access: public
definition: Scar25 ATG

BBa_K1321341
type: Component
persistent identity: component2415489
display id: component2415489
version: 1
top level: BBa_K1321356
access: public
definition: BBa_K1321341

BBa_J04500
type: Component
persistent identity: component2415481
display id: component2415481
version: 1
top level: BBa_K1321356
access: public
definition: BBa_J04500

BBa_K1321341
type: SequenceAnnotation
persistent identity: annotation2415489
display id: annotation2415489
version: 1
top level: BBa_K1321356
location: https://synbiohub.org/public/igem/BBa_K1321356/annotation2415489/range2415489/1
component: BBa_K1321341

https://synbiohub.org/public/igem/BBa_K1321356/annotation2415489/range2415489/1
type: Range
persistent identity: range2415489
display id: range2415489
version: 1
top level: BBa_K1321356
direction: direction/reverse
start: 236
end: 1252
orientation: inline

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2415481
display id: annotation2415481
version: 1
top level: BBa_K1321356
location: https://synbiohub.org/public/igem/BBa_K1321356/annotation2415481/range2415481/1
component: BBa_J04500

https://synbiohub.org/public/igem/BBa_K1321356/annotation2415481/range2415481/1
type: Range
persistent identity: range2415481
display id: range2415481
version: 1
top level: BBa_K1321356
direction: direction/reverse
start: 1
end: 220
orientation: inline

BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2415483
display id: annotation2415483
version: 1
top level: BBa_K1321356
location: https://synbiohub.org/public/igem/BBa_K1321356/annotation2415483/range2415483/1
component: BBa_K1319106

https://synbiohub.org/public/igem/BBa_K1321356/annotation2415483/range2415483/1
type: Range
persistent identity: range2415483
display id: range2415483
version: 1
top level: BBa_K1321356
direction: direction/reverse
start: 221
end: 235
orientation: inline

CBDclos
type: ComponentDefinition
persistent identity: BBa K863111
display id: BBa_K863111
version: 1
was generated by: iGEM to SBOL conversion
description: Cellulose binding domain of C. cellulovorans cellulose binding protein gene (Freiburg-Standard)
created: 2012-09-17T11:00:00Z
modified: 2015-06-17T01:18:55Z
mutable provenance: Cloning-Vector
owned by: james
owned by: myers
mutable description: Cellulose binding Domain of Clostridium cellulovorans cellulose binding protein gene (cbp A) http://www.ncbi.nlm.nih.gov/nuccore/M73817
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 4206
owner id: 13080
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The sequence starts with the Freiburg-prefix (ATG+NgoMIV-restriction-site) followed by 12 Bases (4 AS) of the cellulose binding protein gene in front of the CBD; then 12 bases (4 amino acids) of the cellulose binding protein gene behind the CBD; after that the sequence continues with a short GS-Linker and ends with the Freiburg-suffix (AgeI-restirction-site+TAA).
star: false
creator: Moritz M??ller
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: NgoMIV
sequence annotation: ATG
sequence annotation: GS-Linker
sequence annotation: Siltent mutation (A original)
sequence annotation: AgeI
sequence annotation: TAA
sequence annotation: Cellulose Binding Protein
sequence annotation: Cellulose binding domain (CBDclos)
sequence: https://synbiohub.org/public/igem/BBa_K863111_sequence/1

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2185120
display id: annotation2185120
version: 1
top level: CBDclos
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863111/annotation2185120/range2185120/1

https://synbiohub.org/public/igem/BBa_K863111/annotation2185120/range2185120/1
type: Range
persistent identity: range2185120
display id: range2185120
version: 1
top level: CBDclos
direction: direction/forward
start: 4
end: 9
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2185119
display id: annotation2185119
version: 1
top level: CBDclos
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863111/annotation2185119/range2185119/1

https://synbiohub.org/public/igem/BBa_K863111/annotation2185119/range2185119/1
type: Range
persistent identity: range2185119
display id: range2185119
version: 1
top level: CBDclos
direction: direction/forward
start: 1
end: 3
orientation: inline

GS-Linker
type: SequenceAnnotation
persistent identity: annotation2185127
display id: annotation2185127
version: 1
top level: CBDclos
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863111/annotation2185127/range2185127/1

https://synbiohub.org/public/igem/BBa_K863111/annotation2185127/range2185127/1
type: Range
persistent identity: range2185127
display id: range2185127
version: 1
top level: CBDclos
direction: direction/forward
start: 304
end: 309
orientation: inline

Siltent mutation (A original)
type: SequenceAnnotation
persistent identity: annotation2202131
display id: annotation2202131
version: 1
top level: CBDclos
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K863111/annotation2202131/range2202131/1

https://synbiohub.org/public/igem/BBa_K863111/annotation2202131/range2202131/1
type: Range
persistent identity: range2202131
display id: range2202131
version: 1
top level: CBDclos
direction: direction/forward
start: 93
end: 93
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2185123
display id: annotation2185123
version: 1
top level: CBDclos
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863111/annotation2185123/range2185123/1

https://synbiohub.org/public/igem/BBa_K863111/annotation2185123/range2185123/1
type: Range
persistent identity: range2185123
display id: range2185123
version: 1
top level: CBDclos
direction: direction/forward
start: 310
end: 315
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2185122
display id: annotation2185122
version: 1
top level: CBDclos
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863111/annotation2185122/range2185122/1

https://synbiohub.org/public/igem/BBa_K863111/annotation2185122/range2185122/1
type: Range
persistent identity: range2185122
display id: range2185122
version: 1
top level: CBDclos
direction: direction/forward
start: 316
end: 318
orientation: inline

Cellulose Binding Protein
type: SequenceAnnotation
persistent identity: annotation2185126
display id: annotation2185126
version: 1
top level: CBDclos
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863111/annotation2185126/range2185126/1

https://synbiohub.org/public/igem/BBa_K863111/annotation2185126/range2185126/1
type: Range
persistent identity: range2185126
display id: range2185126
version: 1
top level: CBDclos
direction: direction/forward
start: 298
end: 303
orientation: inline

Cellulose binding domain (CBDclos)
type: SequenceAnnotation
persistent identity: annotation2185124
display id: annotation2185124
version: 1
top level: CBDclos
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K863111/annotation2185124/range2185124/1

https://synbiohub.org/public/igem/BBa_K863111/annotation2185124/range2185124/1
type: Range
persistent identity: range2185124
display id: range2185124
version: 1
top level: CBDclos
direction: direction/forward
start: 22
end: 297
orientation: inline

Cellulose Binding Protein
type: SequenceAnnotation
persistent identity: annotation2185121
display id: annotation2185121
version: 1
top level: CBDclos
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863111/annotation2185121/range2185121/1

https://synbiohub.org/public/igem/BBa_K863111/annotation2185121/range2185121/1
type: Range
persistent identity: range2185121
display id: range2185121
version: 1
top level: CBDclos
direction: direction/forward
start: 10
end: 21
orientation: inline

BBa_K1342003
type: ComponentDefinition
persistent identity: BBa K1342003
display id: BBa_K1342003
version: 1
was generated by: iGEM to SBOL conversion
description: SmtA Part
created: 2014-10-02T11:00:00Z
modified: 2015-07-23T03:03:59Z
mutable provenance: it
owned by: james
owned by: myers
mutable description: This
discontinued: false
dominant: false
experience: experience/None
group u list: _1717_
m user id: 4206
owner id: 22434
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: soon
star: false
creator: Naoki Saito,Daiki Haraguchi,Yoshiharu Otaki,Ryuhei Minei
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: protein
sequence: https://synbiohub.org/public/igem/BBa_K1342003_sequence/1

protein
type: SequenceAnnotation
persistent identity: annotation2394007
display id: annotation2394007
version: 1
top level: BBa_K1342003
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1342003/annotation2394007/range2394007/1

https://synbiohub.org/public/igem/BBa_K1342003/annotation2394007/range2394007/1
type: Range
persistent identity: range2394007
display id: range2394007
version: 1
top level: BBa_K1342003
direction: direction/forward
start: 1
end: 171
orientation: inline

BBa_K1438035
type: ComponentDefinition
persistent identity: BBa K1438035
display id: BBa_K1438035
version: 1
was generated by: iGEM to SBOL conversion
description: PPMT
created: 2014-10-29T12:00:00Z
modified: 2015-07-22T02:15:21Z
mutable provenance: Pseudomonas Putida Metallothioneine
owned by: james
owned by: myers
mutable description: PPMT
discontinued: false
dominant: false
group u list: _1816_
m user id: 4206
owner id: 22762
owning group id: 9
sample status: No part sequence
status: status/Planning
bookmark: false
mutable notes: basic part
star: false
creator: Sascha Kaufmann
type: DnaRegion
role: partType/Coding
role: SO:0000316

BBa_K1321096
type: ComponentDefinition
persistent identity: BBa K1321096
display id: BBa_K1321096
version: 1
was generated by: iGEM to SBOL conversion
description: Linker-CBDcipA-Linker + Phytochelatin (PC) EC20
created: 2014-10-16T11:00:00Z
modified: 2015-06-17T12:35:09Z
mutable provenance: Please see part pages for the composite parts
owned by: james
owned by: myers
mutable description: Synthetic phytochelatin EC20 (metal binding peptide) fused C-terminally to CBDcipA (a cellulose-binding domain), which contains an endogenous N and C-terminal linker sequence. At present the site-directed mutagenesis for this construct is in progress to correct an illegal EcoRI site which was identified in the CBDcipA. This construct is part of a library of fusions with cellulose binding domains which we designed to bind to cellulose and enable capture of heavy metals ([http://2014.igem.org/Team:Imperial/Functionalisation project page]). Other fusion parts with this metal binding protein can be seen in the table below: Note that the start and stop codon, plus 6 bp either side of the sequence, are included the RFC25 prefix and suffix which is not shown.
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
owner id: 20780
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The linker sequences mean fusions should be fine either N or N-terminally to the CBDcipA
star: false
creator: Xenia Spencer-Milnes
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321014
component: BBa_B0105
component: BBa_K1321005
sequence annotation: BBa_K1321005
sequence annotation: BBa_K1321014
sequence annotation: BBa_B0105
sequence: https://synbiohub.org/public/igem/BBa_K1321096_sequence/1

BBa_K1321014
type: Component
persistent identity: component2423603
display id: component2423603
version: 1
top level: BBa_K1321096
access: public
definition: BBa_K1321014

BBa_B0105
type: Component
persistent identity: component2423605
display id: component2423605
version: 1
top level: BBa_K1321096
access: public
definition: Scar 25

BBa_K1321005
type: Component
persistent identity: component2423607
display id: component2423607
version: 1
top level: BBa_K1321096
access: public
definition: BBa_K1321005

BBa_K1321005
type: SequenceAnnotation
persistent identity: annotation2423607
display id: annotation2423607
version: 1
top level: BBa_K1321096
location: https://synbiohub.org/public/igem/BBa_K1321096/annotation2423607/range2423607/1
component: BBa_K1321005

https://synbiohub.org/public/igem/BBa_K1321096/annotation2423607/range2423607/1
type: Range
persistent identity: range2423607
display id: range2423607
version: 1
top level: BBa_K1321096
direction: direction/reverse
start: 718
end: 840
orientation: inline

BBa_K1321014
type: SequenceAnnotation
persistent identity: annotation2423603
display id: annotation2423603
version: 1
top level: BBa_K1321096
location: https://synbiohub.org/public/igem/BBa_K1321096/annotation2423603/range2423603/1
component: BBa_K1321014

https://synbiohub.org/public/igem/BBa_K1321096/annotation2423603/range2423603/1
type: Range
persistent identity: range2423603
display id: range2423603
version: 1
top level: BBa_K1321096
direction: direction/reverse
start: 1
end: 711
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2423605
display id: annotation2423605
version: 1
top level: BBa_K1321096
location: https://synbiohub.org/public/igem/BBa_K1321096/annotation2423605/range2423605/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321096/annotation2423605/range2423605/1
type: Range
persistent identity: range2423605
display id: range2423605
version: 1
top level: BBa_K1321096
direction: direction/reverse
start: 712
end: 717
orientation: inline

BBa_K190019
type: ComponentDefinition
persistent identity: BBa K190019
display id: BBa_K190019
version: 1
was generated by: iGEM to SBOL conversion
description: fMT
created: 2009-08-18T11:00:00Z
modified: 2016-01-13T10:21:29Z
mutable provenance: fMT was cloned from pMAL-MT (kindly provided by Wilfred Chen, University of California), the origin of the gene is the genome of Fucus vesiculosus.
owned by: james
owned by: myers
mutable description: fMT is a metallothionein binding Arsenite (III) and Arsenate(V), used to accumulate As when overexpressed. Binds As (III) stronger than As(V) Reference: Highly Selective and Rapid Arsenic Removal by Metabolically Engineered Escherichia coli Cells Expressing Fucus vesiculosus Metallothionein. Singh et al. App and Env Microbiology, May 2008, p. 2924???2927
discontinued: false
dominant: false
experience: experience/Issues
group u list: _306_
m user id: 4206
owner id: 4147
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: Add BioBrick prefix and suffix, add RBS (B0034)
star: false
creator: Nienke Kuipers
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: fMT
sequence annotation: B0034`
sequence: https://synbiohub.org/public/igem/BBa_K190019_sequence/1

fMT
type: SequenceAnnotation
persistent identity: annotation2017543
display id: annotation2017543
version: 1
top level: BBa_K190019
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K190019/annotation2017543/range2017543/1

https://synbiohub.org/public/igem/BBa_K190019/annotation2017543/range2017543/1
type: Range
persistent identity: range2017543
display id: range2017543
version: 1
top level: BBa_K190019
direction: direction/forward
start: 19
end: 222
orientation: inline

B0034`
type: SequenceAnnotation
persistent identity: annotation2017542
display id: annotation2017542
version: 1
top level: BBa_K190019
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K190019/annotation2017542/range2017542/1

https://synbiohub.org/public/igem/BBa_K190019/annotation2017542/range2017542/1
type: Range
persistent identity: range2017542
display id: range2017542
version: 1
top level: BBa_K190019
direction: direction/forward
start: 1
end: 18
orientation: inline

MBP
type: ComponentDefinition
persistent identity: BBa K231000
display id: BBa_K231000
version: 1
was generated by: iGEM to SBOL conversion
description: Metal binding peptide
created: 2009-10-20T11:00:00Z
modified: 2015-07-21T02:28:52Z
mutable provenance: Derived from peptide described in: Pazirandeh et al. Development of Bacterium-Based Heavy Metal Biosorbents: Enhanced Uptake of Cadmium and Mercury by Escherichia coli Expressing a Metal Binding Motif. Applied and Environmental Microbiology, October 1998, p. 4068-4072, Vol. 64, No. 10
owned by: james
owned by: myers
mutable description: This part codes for a peptide containing a metal binding motif. De novo design allows this peptide to have high affinity for metals. Affinity derives from Cys-Gly repeats. This peptide encodes two Cys-Gly-Cys-Cys-Gly repeats. Other metals besides arsenic, such as Cd and Hg, should be bound as well.
discontinued: false
dominant: false
experience: experience/None
group u list: _328_
m user id: 4206
owner id: 2922
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: This peptide only contains two repeats of the metal binding motif due to size limitations when ordering oligos. Further repeats of the binding motif may enhance its function.
star: false
creator: Daniel R Tarjan
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K231000_sequence/1

Csp1sfGFP
type: ComponentDefinition
persistent identity: BBa K1980001
display id: BBa_K1980001
version: 1
was generated by: iGEM to SBOL conversion
description: TAT Copper Storage Protein 1 sfGFP
created: 2016-10-10T11:00:00Z
modified: 2016-10-12T02:35:29Z
mutable provenance: The source organism for the main protein sequence is Methylosinus trichosporium OB3b. The TAT sequence originate from E. coli multi copper oxidase enzyme CueO. We ordered it as codon optimised DNA from IDT.
owned by: james
owned by: myers
mutable description: Copper Storage protein 1 (Csp1) is a tetrameric copper storage protein found in the periplasm of Methylosinus trichosporium OB3b. We investigated whether this part could act as a copper chelator when expressed in E. coli. We modified the protein by adding a TAT signal peptide from the E. coli enzyme CueO in place of the native TAT sequence and added a c terminal sfGFP fluorescence protein with a C terminal his tag in an attempt to direct the protein to the periplasm. The Csp1 and sfGFP are separated by a short hydrophilic, flexible linker.
discontinued: false
dominant: false
experience: experience/Issues
group u list: _2447_
m user id: 29607
owner id: 29607
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Codon optimised for E. coli. linker added in order to allow the Csp1 and sfGFP to ideally fold separately. Hexahistidine purification tag on c-terminus to maintain TAT sequence.
star: false
creator: Sam Garforth
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Double stop
sequence annotation: TAT signal peptide
sequence annotation: Csp1
sequence annotation: linker
sequence annotation: sfGFP
sequence annotation: Hexahistidine
sequence: https://synbiohub.org/public/igem/BBa_K1980001_sequence/1

Double stop
type: SequenceAnnotation
persistent identity: annotation2495238
display id: annotation2495238
version: 1
top level: Csp1sfGFP
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1980001/annotation2495238/range2495238/1

https://synbiohub.org/public/igem/BBa_K1980001/annotation2495238/range2495238/1
type: Range
persistent identity: range2495238
display id: range2495238
version: 1
top level: Csp1sfGFP
direction: direction/forward
start: 1198
end: 1203
orientation: inline

TAT signal peptide
type: SequenceAnnotation
persistent identity: annotation2495227
display id: annotation2495227
version: 1
top level: Csp1sfGFP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1980001/annotation2495227/range2495227/1

https://synbiohub.org/public/igem/BBa_K1980001/annotation2495227/range2495227/1
type: Range
persistent identity: range2495227
display id: range2495227
version: 1
top level: Csp1sfGFP
direction: direction/forward
start: 1
end: 84
orientation: inline

Csp1
type: SequenceAnnotation
persistent identity: annotation2495228
display id: annotation2495228
version: 1
top level: Csp1sfGFP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1980001/annotation2495228/range2495228/1

https://synbiohub.org/public/igem/BBa_K1980001/annotation2495228/range2495228/1
type: Range
persistent identity: range2495228
display id: range2495228
version: 1
top level: Csp1sfGFP
direction: direction/forward
start: 85
end: 450
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation2495229
display id: annotation2495229
version: 1
top level: Csp1sfGFP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1980001/annotation2495229/range2495229/1

https://synbiohub.org/public/igem/BBa_K1980001/annotation2495229/range2495229/1
type: Range
persistent identity: range2495229
display id: range2495229
version: 1
top level: Csp1sfGFP
direction: direction/forward
start: 451
end: 468
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2495230
display id: annotation2495230
version: 1
top level: Csp1sfGFP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1980001/annotation2495230/range2495230/1

https://synbiohub.org/public/igem/BBa_K1980001/annotation2495230/range2495230/1
type: Range
persistent identity: range2495230
display id: range2495230
version: 1
top level: Csp1sfGFP
direction: direction/forward
start: 469
end: 1179
orientation: inline

Hexahistidine
type: SequenceAnnotation
persistent identity: annotation2495231
display id: annotation2495231
version: 1
top level: Csp1sfGFP
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1980001/annotation2495231/range2495231/1

https://synbiohub.org/public/igem/BBa_K1980001/annotation2495231/range2495231/1
type: Range
persistent identity: range2495231
display id: range2495231
version: 1
top level: Csp1sfGFP
direction: direction/forward
start: 1180
end: 1197
orientation: inline

BBa_K1321115
type: ComponentDefinition
persistent identity: BBa K1321115
display id: BBa_K1321115
version: 1
was generated by: iGEM to SBOL conversion
description: CBDcex fused to phytochelatin (PC) EC20 with LacI promoter
created: 2014-10-08T11:00:00Z
modified: 2015-06-17T12:20:04Z
mutable provenance: CBD7+PhytoC with LacI
owned by: james
owned by: myers
mutable description: CBD7+PhytoC with LacI
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20780
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: CBD7+PhytoC with LacI
star: false
creator: Xenia Spencer-Milnes
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321003
component: BBa_K1319106
component: BBa_B0105
component: BBa_K1321005
component: BBa_J04500
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321005
sequence annotation: BBa_K1319106
sequence annotation: BBa_K1321003
sequence annotation: BBa_J04500
sequence: https://synbiohub.org/public/igem/BBa_K1321115_sequence/1

BBa_K1321003
type: Component
persistent identity: component2414981
display id: component2414981
version: 1
top level: BBa_K1321115
access: public
definition: BBa_K1321003

BBa_K1319106
type: Component
persistent identity: component2414980
display id: component2414980
version: 1
top level: BBa_K1321115
access: public
definition: Scar25 ATG

BBa_B0105
type: Component
persistent identity: component2414983
display id: component2414983
version: 1
top level: BBa_K1321115
access: public
definition: Scar 25

BBa_K1321005
type: Component
persistent identity: component2414985
display id: component2414985
version: 1
top level: BBa_K1321115
access: public
definition: BBa_K1321005

BBa_J04500
type: Component
persistent identity: component2414978
display id: component2414978
version: 1
top level: BBa_K1321115
access: public
definition: BBa_J04500

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2414983
display id: annotation2414983
version: 1
top level: BBa_K1321115
location: https://synbiohub.org/public/igem/BBa_K1321115/annotation2414983/range2414983/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321115/annotation2414983/range2414983/1
type: Range
persistent identity: range2414983
display id: range2414983
version: 1
top level: BBa_K1321115
direction: direction/reverse
start: 563
end: 568
orientation: inline

BBa_K1321005
type: SequenceAnnotation
persistent identity: annotation2414985
display id: annotation2414985
version: 1
top level: BBa_K1321115
location: https://synbiohub.org/public/igem/BBa_K1321115/annotation2414985/range2414985/1
component: BBa_K1321005

https://synbiohub.org/public/igem/BBa_K1321115/annotation2414985/range2414985/1
type: Range
persistent identity: range2414985
display id: range2414985
version: 1
top level: BBa_K1321115
direction: direction/reverse
start: 569
end: 691
orientation: inline

BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2414980
display id: annotation2414980
version: 1
top level: BBa_K1321115
location: https://synbiohub.org/public/igem/BBa_K1321115/annotation2414980/range2414980/1
component: BBa_K1319106

https://synbiohub.org/public/igem/BBa_K1321115/annotation2414980/range2414980/1
type: Range
persistent identity: range2414980
display id: range2414980
version: 1
top level: BBa_K1321115
direction: direction/reverse
start: 221
end: 235
orientation: inline

BBa_K1321003
type: SequenceAnnotation
persistent identity: annotation2414981
display id: annotation2414981
version: 1
top level: BBa_K1321115
location: https://synbiohub.org/public/igem/BBa_K1321115/annotation2414981/range2414981/1
component: BBa_K1321003

https://synbiohub.org/public/igem/BBa_K1321115/annotation2414981/range2414981/1
type: Range
persistent identity: range2414981
display id: range2414981
version: 1
top level: BBa_K1321115
direction: direction/reverse
start: 236
end: 562
orientation: inline

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2414978
display id: annotation2414978
version: 1
top level: BBa_K1321115
location: https://synbiohub.org/public/igem/BBa_K1321115/annotation2414978/range2414978/1
component: BBa_J04500

https://synbiohub.org/public/igem/BBa_K1321115/annotation2414978/range2414978/1
type: Range
persistent identity: range2414978
display id: range2414978
version: 1
top level: BBa_K1321115
direction: direction/reverse
start: 1
end: 220
orientation: inline

BBa_K1933004
type: ComponentDefinition
persistent identity: BBa K1933004
display id: BBa_K1933004
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter and RBS
created: 2016-10-09T11:00:00Z
modified: 2016-10-10T09:38:21Z
mutable provenance: BBa_J23105 was designed by John Anderson and BBa_B0034 was designed by Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. This sequence was chemically synthesized.
owned by: james
owned by: myers
mutable description: This part codes costitutive promoter(BBa_J23105) and RBS(BBa_B0034). Most parts were expressioned by this part in our project.
discontinued: false
dominant: false
experience: experience/Works
group u list: _2400_
m user id: 29998
owner id: 29998
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: 7bp after promoter and 6bp after RBS
star: false
creator: Tomoki Uchino
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: scar
sequence annotation: constitutive promoter and RBS
sequence annotation: Re-entry of BBa_J23105
sequence annotation: nonsense sequence
sequence annotation: Re-entry of BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1933004_sequence/1

scar
type: SequenceAnnotation
persistent identity: annotation2490405
display id: annotation2490405
version: 1
top level: BBa_K1933004
role: feature/scar
location: https://synbiohub.org/public/igem/BBa_K1933004/annotation2490405/range2490405/1

https://synbiohub.org/public/igem/BBa_K1933004/annotation2490405/range2490405/1
type: Range
persistent identity: range2490405
display id: range2490405
version: 1
top level: BBa_K1933004
direction: direction/forward
start: 55
end: 60
orientation: inline

constitutive promoter and RBS
type: SequenceAnnotation
persistent identity: annotation2490408
display id: annotation2490408
version: 1
top level: BBa_K1933004
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1933004/annotation2490408/range2490408/1

https://synbiohub.org/public/igem/BBa_K1933004/annotation2490408/range2490408/1
type: Range
persistent identity: range2490408
display id: range2490408
version: 1
top level: BBa_K1933004
direction: direction/forward
start: 1
end: 60
orientation: inline

Re-entry of BBa_J23105
type: SequenceAnnotation
persistent identity: annotation2490323
display id: annotation2490323
version: 1
top level: BBa_K1933004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1933004/annotation2490323/range2490323/1

https://synbiohub.org/public/igem/BBa_K1933004/annotation2490323/range2490323/1
type: Range
persistent identity: range2490323
display id: range2490323
version: 1
top level: BBa_K1933004
direction: direction/forward
start: 1
end: 35
orientation: inline

nonsense sequence
type: SequenceAnnotation
persistent identity: annotation2490322
display id: annotation2490322
version: 1
top level: BBa_K1933004
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1933004/annotation2490322/range2490322/1

https://synbiohub.org/public/igem/BBa_K1933004/annotation2490322/range2490322/1
type: Range
persistent identity: range2490322
display id: range2490322
version: 1
top level: BBa_K1933004
direction: direction/forward
start: 36
end: 42
orientation: inline

Re-entry of BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2490404
display id: annotation2490404
version: 1
top level: BBa_K1933004
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1933004/annotation2490404/range2490404/1

https://synbiohub.org/public/igem/BBa_K1933004/annotation2490404/range2490404/1
type: Range
persistent identity: range2490404
display id: range2490404
version: 1
top level: BBa_K1933004
direction: direction/forward
start: 43
end: 54
orientation: inline

https://synbiohub.org/public/igem/BBa_K1321096_sequence/1
type: Sequence
persistent identity: BBa K1321096 sequence
display id: BBa_K1321096_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aatgctacgccaactaagggtgcaaccccgaccaacacagcaacgcctacaaaaagcgctacagcaacacctacaagaccgtcagttcctacaaacacaccgactaacacaccggcaaatacacctgtttcaggcaacttgaaggtcgaattctataactcaaatccgagtgatacaactaacagtattaatccgcagtttaaagtaacaaatacaggatcaagtgcaattgatctttcaaagcttacattgagatactattacaccgttgatggccagaaggaccagactttctggtgtgaccatgcagctatcataggtagcaacggctcatacaacggcatcacatcaaatgtaaaaggaacattcgtaaagatgagctcaagcacaaataacgcagacacatacctcgaaataagtttcacaggtggcactttggaacctggtgctcatgtacagatacagggtaggtttgcgaaaaatgactggagtaattatacacagtcaaatgattactcatttaagtcagcatcacagttcgtagaatgggatcaggttacagcatatttgaatggagtacttgtatggggtaaagaaccaggaggatcagtagttccgtcaacacagccggtaacaaccccaccggcaacaaccaagccgccagcaacaaccaaaccaccggctaccacgattcctccatcagacgatccgaccggcgaatgtgaatgtgagtgcgaatgcgagtgtgaatgtgagtgtgagtgtgaatgtgaatgcgagtgtgagtgcgagtgcgaatgtgagtgtgaatgcgagtgcgaatgcgaatgcgagtgcggc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1478001_sequence/1
type: Sequence
persistent identity: BBa K1478001 sequence
display id: BBa_K1478001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaccagttctatgtcagtagaattttataactctaacaaatctgctcagaccaattcaataacgcctatcataaagataacaaatacgagcgactcggatctaaacttgaacgatgtgaaggtgcggtactactacacatcagatgggactcaaggacaaacattctggtgcgatcacgcaggtgcactacttggtaattcgtacgtcgataatacttcaaaagttaccgcgaattttgtcaaagagactgcttcaccaacatcgacttacgacacgtacgttgaatttggatttgcgtcaggccgt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K863110_sequence/1
type: Sequence
persistent identity: BBa K863110 sequence
display id: BBa_K863110_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggccggctcatcaatgtcagttgaattttacaactctaacaaatcagcacaaacaaactcaattacaccaataatcaaaattactaacacatctgacagtgatttaaatttaaatgacgtaaaagttagatattattacacaagtgatggtacacaaggacaaactttctggtgtgaccatgctggtgcattattaggaaatagctatgttgataacactagcaaagtgacagcaaacttcgttaaagaaacagcaagcccaacatcaacctatgatacatatgttgaatttggatttgcaaccggttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1505000_sequence/1
type: Sequence
persistent identity: BBa K1505000 sequence
display id: BBa_K1505000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctactggtaccgctactgtccaagccctatgagggagcctttctagctcactgctcttcgctactatgacgaactatcgcgttgagagtagcagcggacgggcggcgcgcaagatgaggctcgcattaatgggacctgcgttcattgcggcgattggttatatcgatcccggtaactttgcgaccaatattcaggcgggtgcaagtttcggctatcagctactgtgggttgtcgtttgggccaacctgatggcgatgctgattcagatcctctctgccaaactagggattgccactggtaaaaatctggcggagcagattcgcgatcactatccgcgtcccgtagtgtggttctattgggttcaggcagaaattattgcgatggcaaccgacctggcggaatttattggtgcggcgatcggttttaaactcattcttggtgtttcgttgttgcagggcgcggtgctgacggggatcgcgactttcctgattttaatgctgcaacgtcgcgggcaaaaaccgctggagaaagtgattggcgggttactgttgtttgttgccgcggcttacattgtcgagttgattttctcccagcctaacctggcgcagctcggtaaaggaatggtgatcccgagtttacctacttcggaagcggtcttcctggcagcaggcgtgttaggggcgacgattatgccgcatgtgatttatttgcactcctcgctcactcagcatttacatggcggttcgcgtcaacaacgttattccgccaccaaatgggatgtggctatcgccatgactattgccggttttgtcaatctggcgatgatggctacagcagcggcggcgttccacttttccggtcatactggtgttgccgatcttgatgaggcttatctgacgctgcaaccgctgttaagccacgctgcggcaacggtctttggattaagcctggttgctgcggggctgtcttcaacggtggtggggacactggcggggcaggtggtgatgcagggcttcattcgctttcatatcccgctgtgggtgcgtcgtacagtcaccatgttgccgtcatttattgtcattctgatgggattagatccgacacggattctggttatgagtcaggtactgttaagttttggtatcgctctggcgctggttccactgctgattttcaccagtgacagcaagttgatgggcgatctggtgaacagcaaacgcgtaaaacagacaggctgggtgattgtggtgctggtcgtggcgctgaatatctggttgttggtggggacggcgctgggattggtcgaagtgcatgcgtggtcgt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K231000_sequence/1
type: Sequence
persistent identity: BBa K231000 sequence
display id: BBa_K231000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtgttgtggttgcggtaaaggtcattgttgtggttgtggtaaaggtcat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1420004_sequence/1
type: Sequence
persistent identity: BBa K1420004 sequence
display id: BBa_K1420004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctacaccgcgtcggcaccacgcggctctttctccccttgcagcgaagcaatcagcgggcaagaaacgttcccttgccgcgcatggcaggcgaacacaagttcggatagcacggtttccatgcgcgccaggtcggtcattttttcgcgcacgtcctgaagcttgtgctcggccaggctgctggcttcctcgcagtgggtgccgtcatccagcctcagcagctctgcgatctcgtcgaggctgaatccgagccgctgggctgatttcacgaagcgcacccgcgtcacatccgcctcgccatagcggcggatgctgccatagggcttgtccggctccggcaacaagcccttgcgctgatagaaccggattgtttccacgttgaccccggccgccttggcgaaaacgccaatagtcagattctccaaatttttttccat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K346001_sequence/1
type: Sequence
persistent identity: BBa K346001 sequence
display id: BBa_K346001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggaaaataatttggaaaacctgaccattggcgtttttgccaaggcggccggggtcaacgtggagacaatccgcttctatcagcgcaagggcctgttgcgggaaccggacaagccttacggcagcatccgccgctatggggaggcggacgtggttcgggtgaaattcgtgaaatcggcacagcggctggggttcagtctggacgagattgccgagctgttgcggctcgacgatggcacccactgcgaggaggccagcagcctggccgaacacaagctcaaggacgtgcgcgagaagatggccgacttggcgcgcatggaaaccgtgctgtctgaactcgtgtgcgcctgccatgcacgaaaggggaatgtttcctgcccgttgatcgcgtcactacagggcgaagcaggcctggcaaggtcagctatgccttag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1420003_sequence/1
type: Sequence
persistent identity: BBa K1420003 sequence
display id: BBa_K1420003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaaactgtttgcctctctcgccatcgctgccgttgttgcccccgtgtgggccgccacccagaccgtcacgctgtccgtaccgggcatgacctgctccgcttgtccgatcaccgttaagaaggcgatttccaaggtcgaaggcgtcagcaaagttaacgtgaccttcgagacacgcgaagcggttgtcaccttcgatgatgccaagaccagcgtgcagaagctgaccaaggccaccgaagacgcgggctatccgtccagcgtcaagaagtga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1321353_sequence/1
type: Sequence
persistent identity: BBa K1321353 sequence
display id: BBa_K1321353_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggagatactagagaaagaggagaaatactagatggccggcgcacaccatgaagaacaacacggcggacaccaccaccatcaccatcacaacaccaccaccactaccatggcggcgaacaccaccatcaccaccacagctctcatcatgaagaaggttgttgtagcactagcgatagtcatcatcaagaagaaggttgctgccacgggcatcacgagaccggctcatcaatgtcagttgaattttacaactctaacaaatcagcacaaacaaactcaattacaccaataatcaaaattactaacacgtctgacagtgatttaaatttaaatgacgtaaaagttagatattattacacaagtgatggtacacaaggacaaactttctggtgtgaccatgctggtgcattattaggaaatagctatgttgataacactagcaaagtgacagcaaacttcgttaaagaaacagcaagcccaacatcaacctatgatacatatgttgaatttggatttgcaggcagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1321340_sequence/1
type: Sequence
persistent identity: BBa K1321340 sequence
display id: BBa_K1321340_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: cccggcgccaatccgcccggtactacgaccacttcgcgtccagcaaccacgaccgggtcgagccccggcccacaggcctgttcttccgtctggggtcagtgcggcggacagaactggtctggccctacctgctgtgcttctggcagcacatgcgtctactctaacgactactatagccagtgcctgccgggtgctaacccgccaggcactaccactacctctcgccccgctaccactacaggctctagcccgggacctacccaaagccactacggccagtgcggaggcattggctacagcggccctaccgtctgcgcctccggaaccacctgccaagtcctgaacccgtactactcccagtgtctt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1122703_sequence/1
type: Sequence
persistent identity: BBa K1122703 sequence
display id: BBa_K1122703_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggacattaccgtgtacaacggccaacacaaagaagcggcacaagccgttgcagatgcctttacccgggctaccggcatcaaagtcaaactcaacagtgccaaaggcgaccagcttgccggccaaatcaaagaagaaggcagccgaagccccgccgacgtattctattccgaacaaatcccggcactcgccaccctttccgcagccaacctcctagagcccctgcccgcctccaccatcaacgaaacacgcggcaaaggcgtgccggttgccgccaaaaaagactgggtggcactgagcggacgttcgcgcgtcgtcgtttacgacacccgcaaactgtctgaaaaagatttggaaaaatccgtcctgaattacgccacgccgaaatggaaaaaccgcatcggttacgtccccacttccggcgcgttcttggaacagattgtcgccatcgtcaaactgaaaggcgaagcggccgcattgaaatggctcaaaggcctgaaagaatacggcaagccttacgctaaaaactccgtcgcccttcaagcggttgaaaacggcgaaatcgatgccgccctcatcaacaactactactggcacgctttcgcgcgtgaaaaaggcgtacaaaatgtccacacccgcctgaatttcgtccgccacagagatcccggcgcactcgttacctattccggcgcagccgtgttaaaatcctcccaaaacaaggatgaggcgaaaaaattcgtcgccttcctcgccggcaaggaaggacagcgcgccctgaccgccgtccgtgccgaatatcctttgaatccgcacgtggtatccaccttcaatttggaacccatcgccaagttggaagcaccccaagtgtccgccaccactgtttccgaaaaagaacacgccacccggctgcttgagcaagccggtatgaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1526007_sequence/1
type: Sequence
persistent identity: BBa K1526007 sequence
display id: BBa_K1526007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagatactagaaagaggagaaatactagatgacaaactatcgcgtggaatcaagctcgggtcgcgccgcacgcaaaatgcgtctggccctgatgggcccagcctttattgcggcgattggttatatcgatcctggtaacttcgcgactaacatccaagcaggtgcctcttttgggtatcagttgctgtgggtagtggtttgggccaatctgatggcgatgctgatccagattttgtcggcgaaactggggattgcgacaggcaagaatttagccgaacagatccgcgatcactacccacggccggtagtttggttctactgggttcaggcggaaatcatcgcgatggcaaccgatttggcggaatttattggcgctgctatcggctttaaactgatcctgggtgtctcgcttctacagggcgccgtgctgaccggcatcgccaccttcctgattttaatgctacagcgccggggccagaaaccactggagaaggtgatcgggggactgcttcttttcgtcgcagccgcctatatcgtggagctgatttttagtcagccgaacctggcgcaactggggaagggcatggtcattccg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K190019_sequence/1
type: Sequence
persistent identity: BBa K190019 sequence
display id: BBa_K190019_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggcgggcactggctgcaagatctgggaagactgcaagtgcggagcggcgtgcagctgcggcgactcgtgcacctgcggaactgtcaagaagggcaccacctctcgcgccggcgcgggctgcccctgcggccccaagtgcaaatgcaccggccaaggcagctgcaactgcgtcaaggacgactgctgcggctgcggcaagtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1321090_sequence/1
type: Sequence
persistent identity: BBa K1321090 sequence
display id: BBa_K1321090_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaatgtgaatgtgagtgcgaatgcgagtgtgaatgtgagtgtgagtgtgaatgtgaatgcgagtgtgagtgcgagtgcgaatgtgagtgtgaatgcgagtgcgaatgcgaatgcgagtgcggcaccggccccggcgccaatccgcccggtactacgaccacttcgcgtccagcaaccacgaccgggtcgagccccggcccacaggcctgttcttccgtctggggtcagtgcggcggacagaactggtctggccctacctgctgtgcttctggcagcacatgcgtctactctaacgactactatagccagtgcctgccgggtgctaacccgccaggcactaccactacctctcgccccgctaccactacaggctctagcccgggacctacccaaagccactacggccagtgcggaggcattggctacagcggccctaccgtctgcgcctccggaaccacctgccaagtcctgaacccgtactactcccagtgtctt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1980003_sequence/1
type: Sequence
persistent identity: BBa K1980003 sequence
display id: BBa_K1980003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtgtaattcgcatgactaattatgaagccggtacacttttgacttgtagccacgaggggtgcggctgtcgtgttcgtattgaagtcccgtgtcattgtgccggagcgggcgacgcttaccgctgcacctgtggagatgaactggctcctgtgaaaggcagtgggtctgggtcgagcaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtccgtggagagggtgaaggtgatgctacaaacggaaaactcacccttaaatttatttgcactactggaaaactacctgttccctggccaacacttgtcactactctgacctatggtgttcaatgcttttcccgttatccggatcacatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggacctacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaaaggtattgattttaaagaagatggaaacattctcggacacaaactcgagtacaactttaactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacgttgaagatggttccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgtcctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaacatcatcaccaccatcactaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1321012_sequence/1
type: Sequence
persistent identity: BBa K1321012 sequence
display id: BBa_K1321012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcaggtacaggttgtaaaatttgggaagattgtaaatgtggtgcagcatgtagctgtggtgatagctgtacctgtggcaccgttaaaaaaggtacaaccagccgtgccggtgcaggttgtccgtgtggtccgaaatgtaaatgtacaggtcagggtagctgtaactgcgttaaagatgattgttgtggttgtggcaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1460003_sequence/1
type: Sequence
persistent identity: BBa K1460003 sequence
display id: BBa_K1460003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtactgtgctagctactagagaaagacaggaccctactagatggccaataacgcttcgttttatgcggcggcgtctatggcttacatgctaggggcaaagcatgcgtttgatgcggatcacatcgcttgcatagacaacaccattagaaagctcacccaacaaggcaaaaacgcctatggtgtggggttttacttttctatggggcattcaagcgtggtgattttaatgaccatcatcagcgcgtttgcgatcgcttgggctaaagaacacacgccgatgctagaagaaatagggggggtagtggggactttagtttctgggctttttttactcattatagggctattaaatgcgattattctcttggatttattaaaaatattcaaaaaatcgcactctaatgaaagcctgagccagcaacaaaatgaagagattgaacggcttttaacgagtaggggcttacttaatcgcttttttaaacccttgtttaattttgtctccaagtcgtggcatatttatcctatcggttttctttttgggttgggttttgataccgctagtgaaatcgcgcttttggccctctctagcagcgcgattaaagtgagtgtggtgggcatgctctctttacccattctttttgccgctggcatgagtttgtttgacactttagatggggcgttcatgctcaaggcgtatgattgggcgttcaaaacccctttaagaaaaatctattacaatatctctatcacggccttaagcgtgtttatcgcgctctttatcgggttgattgagctttttcaagtcgttagcgagaaactccatttaaaatttgaaaaccgccttctaaacaccttacaaagcctagaatttacagactttggttattacttggtgggcttatttgtaatagcgtttctaggctcattctttttatggaaaatcaagttttctaaactagagagctgaaaaaaaaaaccccgcccctgacagggcggggttttttttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1980012_sequence/1
type: Sequence
persistent identity: BBa K1980012 sequence
display id: BBa_K1980012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttattaccctgcgcgatgatgacaacaacccgacaggttttcaataatcggacagtcggcactatcgtcgcccggacacgcgttagccagcgccagcagttggtcccgcatgctctgtaattcttcgatatggcgctcaatttccgcaactttttcaagggtacggcgtttcacatcagcagagtgacgttgcggatcgttgaacaaattgaccagttcaccgctctcctcaaggttgaaccctacttgacgggcctggcgcagtaacgtcaactcattgaggtgctgttgcgtataggtgcgataaccattctctgagcgcatcggcggagtaaccaaacctttttcttcataaaagcggattgctttgctggtcagacctgtgatcttcgccacatcggagatgttcatctgtaatttctcctctttctctagtagctagcacaatacctaggactgagctagctgtcaaactagagagtagatagatagatgcaccccgcctggtttattaatttcttgaccttccccttgctggaaggtttaacctttatcatactagaaagaggagaaatactagatgcgtgtaattcgcatgactaattatgaagccggtacacttttgacttgtagccacgaggggtgcggctgtcgtgttcgtattgaagtcccgtgtcattgtgccggagcgggcgacgcttaccgctgcacctgtggagatgaactggctcctgtgaaaggcagtgggtctgggtcgagcaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtccgtggagagggtgaaggtgatgctacaaacggaaaactcacccttaaatttatttgcactactggaaaactacctgttccctggccaacacttgtcactactctgacctatggtgttcaatgcttttcccgttatccggatcacatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggacctacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaaaggtattgattttaaagaagatggaaacattctcggacacaaactcgagtacaactttaactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacgttgaagatggttccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgtcctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaacatcatcaccaccatcactaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1321011_sequence/1
type: Sequence
persistent identity: BBa K1321011 sequence
display id: BBa_K1321011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: accagcaccaccctggttaaatgtgcatgtgaaccgtgtctgtgtaatgttgatccgagcaaagcaattgatcgcaatggtctgtattattgtagcgaagcatgtgcagatggtcatacaggtggtagcaaaggttgtggtcataccggctgtaattgtcatggc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0105_sequence/1
type: Sequence
persistent identity: BBa B0105 sequence
display id: BBa_B0105_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: accggc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1321122_sequence/1
type: Sequence
persistent identity: BBa K1321122 sequence
display id: BBa_K1321122_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: accagcaccaccctggttaaatgtgcatgtgaaccgtgtctgtgtaatgttgatccgagcaaagcaattgatcgcaatggtctgtattattgtagcgaagcatgtgcagatggtcatacaggtggtagcaaaggttgtggtcataccggctgtaattgtcatggcaccggcggtccggccgggtgccaggtgctgtggggcgtcaaccagtggaacaccggcttcaccgcgaacgtcaccgtgaagaacacgtcctccgctccggtagacggctggacgctcacgttcagcttcccgtccggccagcaggtcacccaggcgtggagctcgacggtcacgcagtccggctcggccgtgacggtccgcaacgccccgtggaacggctcgatcccggcgggcggcaccgcgcagttcggcttcaacggctcgcacacgggcaccaacgccgcgccgacggcgttctcgctcaacggcacgccctgcacggtcggcggcagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K346003_sequence/1
type: Sequence
persistent identity: BBa K346003 sequence
display id: BBa_K346003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgacccactgcgaggaggccagcagcctggccgaacacaagctcaaggacgtgcgcgagaagatggccgacttggcgcgcatggaaaccgtgctgtctgaactcgtgtgcgcctgccatgcacgaaaggggaatgtttcctgcccgttgatcgcgtcactacagggatcctcaggcacccactgcgaggaggccagcagtctggccgaacacaagctcaaggacgtgcgcgagaagatggccgacttggcgcgcatggaaaccgtgctgtctgaactcgtgtgcgcctgccatgcacgaaaggggaatgtttcctgcccgtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1321101_sequence/1
type: Sequence
persistent identity: BBa K1321101 sequence
display id: BBa_K1321101_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggagatactagagaaagaggagaaatactagatggccggcgaatgtgaatgtgagtgcgaatgcgagtgtgaatgtgagtgtgagtgtgaatgtgaatgcgagtgtgagtgcgagtgcgaatgtgagtgtgaatgcgagtgcgaatgcgaatgcgagtgcggcaccggctcatcaatgtcagttgaattttacaactctaacaaatcagcacaaacaaactcaattacaccaataatcaaaattactaacacgtctgacagtgatttaaatttaaatgacgtaaaagttagatattattacacaagtgatggtacacaaggacaaactttctggtgtgaccatgctggtgcattattaggaaatagctatgttgataacactagcaaagtgacagcaaacttcgttaaagaaacagcaagcccaacatcaacctatgatacatatgttgaatttggatttgcaggcagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1460007_sequence/1
type: Sequence
persistent identity: BBa K1460007 sequence
display id: BBa_K1460007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtactgtgctagctactagagaaagacaggaccctactagatgtctgaaccaaaaaccgggcgcggcgcgctcttcactggagggcttgccgccatcctcgcctcggcttgctgcctcgggccgttggttctgatcgccttggggttcagcggcgcttggatcggcaacttggcggtgttggaaccctatcgccccatctttatcggcgtggcgctggtggcgttgttcttcgcctggcggcgcatctaccggcaggcagcggcctgcaaaccgggtgaggtctgcgcgattccccaagtgcgagctacttacaagctcattttctggatcgtggccgcgctggttctggtcgcgctcggatttccctacgtcatgccatttttctactgatcggagttcaccatgaagaaactgtttgcctccctcgccctcgccgccgttgttgcccccgtctgggccgccacccagaccgtcacgctgtccgtaccgggcatgacctgctccgcctgcccgatcactgtcaagaaggcgatttccgaggtcgaaggcgtcagcaaagttgacgtgactttcgagacacgccaagcggtcgtcaccttcgacgatgccaagaccagcgtgcagaagctgaccaaggcaaccgcagacgcgggctatccgtccagcgtcaagcagtgaaaaaaaaaaccccgcccctgacagggcggggttttttttttactagaggtaccgagctcgtaatacgactcactatagggaatacaagctacttgttctttttgcaggtacctactagagaaagacaggaccctactagatgtcccctatactaggttattggaaaattaagggccttgtgcaacccactcgacttcttttggaatatcttgaagaaaaatatgaagagcatttgtatgagcgcgatgaaggtgataaatggcgaaacaaaaagtttgaattgggtttggagtttcccaatcttccttattatattgatggtgatgttaaattaacacagtctatggccatcatacgttatatagctgacaagcacaacatgttgggtggttgtccaaaagagcgtgcagagatttcaatgcttgaaggagcggttttggatattagatacggtgtttcgagaattgcatatagtaaagactttgaaactctcaaagttgattttcttagcaagctacctgaaatgctgaaaatgttcgaagatcgtttatgtcataaaacatatttaaatggtgatcatgtaacccatcctgacttcatgttgtatgacgctcttgatgttgttttatacatggacccaatgtgcctggatgcgttcccaaaattagtttgttttaaaaaacgtattgaagctatcccacaaattgataagtacttgaaatccagcaagtatatagcatggcctttgcagggctggcaagccacgtttggtggtggcgaccatcctccaaaatcggatctggttccgcgtggatccattcatatgatgactgtaaaaatatgtgactgtgaaggcgaatgttgtaaggactcttgtcattgtgggagcacctgccttccaagctgttctggcggtgaaaagtgcaaatgtgatcacagcaccggaagccctcaatgtaagagttgtggtgaaaaatgcaaatgcgaaaccacgtgcacttgtgaaaagagtaaatgcaattgtgaaaaatgttagaaaaaaaaaccccgcccctgacagggcggggttttttttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1321154_sequence/1
type: Sequence
persistent identity: BBa K1321154 sequence
display id: BBa_K1321154_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcatcaatgtcagttgaattttacaactctaacaaatcagcacaaacaaactcaattacaccaataatcaaaattactaacacgtctgacagtgatttaaatttaaatgacgtaaaagttagatattattacacaagtgatggtacacaaggacaaactttctggtgtgaccatgctggtgcattattaggaaatagctatgttgataacactagcaaagtgacagcaaacttcgttaaagaaacagcaagcccaacatcaacctatgatacatatgttgaatttggatttgcaggcagcaccggcgcaggtacaggttgtaaaatttgggaagattgtaaatgtggtgcagcatgtagctgtggtgatagctgtacctgtggcaccgttaaaaaaggtacaaccagccgtgccggtgcaggttgtccgtgtggtccgaaatgtaaatgtacaggtcagggtagctgtaactgcgttaaagatgattgttgtggttgtggcaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1321366_sequence/1
type: Sequence
persistent identity: BBa K1321366 sequence
display id: BBa_K1321366_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggagatactagagaaagaggagaaatactagatggccggcggtccggccgggtgccaggtgctgtggggcgtcaaccagtggaacaccggcttcaccgcgaacgtcaccgtgaagaacacgtcctccgctccggtagacggctggacgctcacgttcagcttcccgtccggccagcaggtcacccaggcgtggagctcgacggtcacgcagtccggctcggccgtgacggtccgcaacgccccgtggaacggctcgatcccggcgggcggcaccgcgcagttcggcttcaacggctcgcacacgggcaccaacgccgcgccgacggcgttctcgctcaacggcacgccctgcacggtcggcggcagcaccggccgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1460008_sequence/1
type: Sequence
persistent identity: BBa K1460008 sequence
display id: BBa_K1460008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtactgtgctagctactagagaaagacaggaccctactagatgaatcaccgccaagacgagtttgtgaggtttcaggattggaaatcagagagaagctctgagggaaattttcatgctaaaggtggagtccaccgaagtaaagttgtaatagcttccgacgaattacataatcgcttggaatctggtaaatggagggccaaaggcataatacaagctgtaaaatctagtttaagtggttttgtggaagaaagcttgggatccaaaaagaacattcttgatcctcaaggaccttttcttcgaaagtggaacaaaatatttgtattatcctgtgtaattgctatctccttggatcctttgttcctttatattccagtgattgataacgacaacaaatgcctggggttaaacagaacgttggaggtgacagctagtgttttgcgttcttttactgatatattttactttcttcatattgcccttcaatttcgaactggttttattgctccttcgtcacgtgtatttggaagaggtgttttaattgaagatgcttgggagatagcaaagagatacttatccacttacttcttgatcgacattcttgcagttctaccactaccacaggttgtaattttaattataattcctaagttgcggggttcaagatctttgaataccaagaatttgctgaagtctgttgtctttttccaatatattccaagggtccttcgagtgtatcctttatatagggaagtcacaagaacatctggcatactcactgaaacagcatgggctggagctgctttcaatctcttcctttatatgcttgccagccatgtacttggagccttttggtatctgttttctatagaacgcgaaactacttgctggaaacaagcttgtggaaattcttctccctgtcatcatgcctcattgtattgtgatgatgatcatacaaaatttaaaacattgctgaacagttcctgccctatagagacaccaaatgcaacgctatttgattttgggatattccttggcgctctccaatcaggtgttgtgggaccaatggattttccgcagaagttcttttattgtttctggtggggtttacaaaatttaagttcccttggtcaaaacctccaaacaagtaccttcatctgggaaatgtgctttgcagttttcatttccatcgctggcctggtgctatttgcctttctcattggcaatatgcagacatgtctgcaatcctcaacattaagattagaggagatgagggtgaaaagacgagatgcagaacagtggatgtcacatcggttgctccctgagaacctcagagagcggattaggtgctatgagcaatacaggtggcaggaaactaggggtgttgatgaagagaatctgattcacaaccttccgaaagacctcaggagagatataaagcgtcatctttgtttagctttgctcatgagagtgcccatgtttgaaaaaatggatgaacaactcttagatgcattatgcgaccatctgaagccagtgctattcaccaaggacagcttcattgttcgtgaaggtgatccagttgatgcgatgctgtttgtaatgaggggcaaactactgtctgtaaccactaatggagggaggacaggtttttttaactctgagcatctaaaagctggtgatttttgtggagaggagctacttacttgggctcttgaccctaattcgtcaactaacctaccaatctcaactagaactgcccaagcactctcagaagttgaagcatttgctttagtggctgatgatttaaagcttgtagcctctcagttcagacgacttcacagtaagcagctccgccacactttcaggttttactcaggccaatggagaacttgggcagcttgcttcatacaagcagcatggcggagttattgcaggaagaatgtagaagagtctcttcgtgatgaagaaaacaggttgcaa…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1980000_sequence/1
type: Sequence
persistent identity: BBa K1980000 sequence
display id: BBa_K1980000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcaacgtcgtgacttcctgaaatacagtgttgctcttggggttgcttccgcattacccctttggtcccgcgcagtgtttgctggtgaagacccgcacgcaggccataagatgtctcatggtgcgaagtacaaagcacttcttgactcttcctcccattgtgttgcggtaggcgaggactgtctgcgtcactgttttgaaatgcttgctatgaacgacgcgagtatgggggcgtgcaccaaagccacctacgatcttgtagccgcctgcggagcattagctaagctggcggggaccaactcagcctttacaccagccttcgccaaagtcgttgctgatgtatgtgccgcgtgcaaaaaggaatgtgacaagtttccatccattgccgaatgtaaggcttgcggagaggcgtgccaggcttgtgcagaagagtgtcacaaagtcgcggcccatcatcaccaccatcactaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1321092_sequence/1
type: Sequence
persistent identity: BBa K1321092 sequence
display id: BBa_K1321092_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaatgtgaatgtgagtgcgaatgcgagtgtgaatgtgagtgtgagtgtgaatgtgaatgcgagtgtgagtgcgagtgcgaatgtgagtgtgaatgcgagtgcgaatgcgaatgcgagtgcggcaccggcggtccggccgggtgccaggtgctgtggggcgtcaaccagtggaacaccggcttcaccgcgaacgtcaccgtgaagaacacgtcctccgctccggtagacggctggacgctcacgttcagcttcccgtccggccagcaggtcacccaggcgtggagctcgacggtcacgcagtccggctcggccgtgacggtccgcaacgccccgtggaacggctcgatcccggcgggcggcaccgcgcagttcggcttcaacggctcgcacacgggcaccaacgccgcgccgacggcgttctcgctcaacggcacgccctgcacggtcggcggcagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1460004_sequence/1
type: Sequence
persistent identity: BBa K1460004 sequence
display id: BBa_K1460004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtactgtgctagctactagagaaagacaggaccctactagatgtctgaaccaaaaaccgggcgcggcgcgctcttcactggagggcttgccgccatcctcgcctcggcttgctgcctcgggccgttggttctgatcgccttggggttcagcggcgcttggatcggcaacttggcggtgttggaaccctatcgccccatctttatcggcgtggcgctggtggcgttgttcttcgcctggcggcgcatctaccggcaggcagcggcctgcaaaccgggtgaggtctgcgcgattccccaagtgcgagctacttacaagctcattttctggatcgtggccgcgctggttctggtcgcgctcggatttccctacgtcatgccatttttctactgatcggagttcaccatgaagaaactgtttgcctccctcgccctcgccgccgttgttgcccccgtctgggccgccacccagaccgtcacgctgtccgtaccgggcatgacctgctccgcctgcccgatcactgtcaagaaggcgatttccgaggtcgaaggcgtcagcaaagttgacgtgactttcgagacacgccaagcggtcgtcaccttcgacgatgccaagaccagcgtgcagaagctgaccaaggcaaccgcagacgcgggctatccgtccagcgtcaagcagtgaaaaaaaaaaccccgcccctgacagggcggggttttttttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1321344_sequence/1
type: Sequence
persistent identity: BBa K1321344 sequence
display id: BBa_K1321344_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcacaccatgaagaacaacacggcggacaccaccaccatcaccatcacaacaccaccaccactaccatggcggcgaacaccaccatcaccaccacagctctcatcatgaagaaggttgttgtagcactagcgatagtcatcatcaagaagaaggttgctgccacgggcatcacgagaccggcggtccggccgggtgccaggtgctgtggggcgtcaaccagtggaacaccggcttcaccgcgaacgtcaccgtgaagaacacgtcctccgctccggtagacggctggacgctcacgttcagcttcccgtccggccagcaggtcacccaggcgtggagctcgacggtcacgcagtccggctcggccgtgacggtccgcaacgccccgtggaacggctcgatcccggcgggcggcaccgcgcagttcggcttcaacggctcgcacacgggcaccaacgccgcgccgacggcgttctcgctcaacggcacgccctgcacggtcggcggcagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1321339_sequence/1
type: Sequence
persistent identity: BBa K1321339 sequence
display id: BBa_K1321339_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcgccgggttgccgtgttgactacgcggttaccaaccagtggccgggtggtttcggtgcgaacgttaccatcaccaacctgggtgacccggtttcttcttggaaactggactggacctacaccgcgggtcagcgtatccagcagctgtggaacggtaccgcgtctaccaacggtggtcaggtttctgttacctctctgccgtggaacggttctatcccgactggtggtaccgcgtctttcggtttcaacggttcttgggcgggttctaacccgaccccggcgtctttctctctgaacggtaccacctgcacgggtaccccgacgacctcgccaactcctacgccgaccccaaccacgccgactcctacaccgacgcccaccccaacgcctactccgacggtcaccccc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1321343_sequence/1
type: Sequence
persistent identity: BBa K1321343 sequence
display id: BBa_K1321343_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcacaccatgaagaacaacacggcggacaccaccaccatcaccatcacaacaccaccaccactaccatggcggcgaacaccaccatcaccaccacagctctcatcatgaagaaggttgttgtagcactagcgatagtcatcatcaagaagaaggttgctgccacgggcatcacgagaccggctcatcaatgtcagttgaattttacaactctaacaaatcagcacaaacaaactcaattacaccaataatcaaaattactaacacgtctgacagtgatttaaatttaaatgacgtaaaagttagatattattacacaagtgatggtacacaaggacaaactttctggtgtgaccatgctggtgcattattaggaaatagctatgttgataacactagcaaagtgacagcaaacttcgttaaagaaacagcaagcccaacatcaacctatgatacatatgttgaatttggatttgcaggcagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1321342_sequence/1
type: Sequence
persistent identity: BBa K1321342 sequence
display id: BBa_K1321342_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaaaccggcggtccggccgggtgccaggtgctgtggggcgtcaaccagtggaacaccggcttcaccgcgaacgtcaccgtgaagaacacgtcctccgctccggtagacggctggacgctcacgttcagcttcccgtccggccagcaggtcacccaggcgtggagctcgacggtcacgcagtccggctcggccgtgacggtccgcaacgccccgtggaacggctcgatcccggcgggcggcaccgcgcagttcggcttcaacggctcgcacacgggcaccaacgccgcgccgacggcgttctcgctcaacggcacgccctgcacggtcggcggcagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1505001_sequence/1
type: Sequence
persistent identity: BBa K1505001 sequence
display id: BBa_K1505001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cttctgggatcccttcatggtccaagccctatgagggagcctttctagtcttgcaattgcagctgatgacctcaacaacgttggtcaaatgcgcttgtgagccctgtctctgcaacgtcgatcccagcaaagcgatcgatcgcaacggtctgtactactgtagcgaagcctgtgccgatggccacaccggaggtagcaaaggctgcggccacaccggctgtaactgccacggcggtggaggcggttcagtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataacagaagcatatgtagaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1980010_sequence/1
type: Sequence
persistent identity: BBa K1980010 sequence
display id: BBa_K1980010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttattaccctgcgcgatgatgacaacaacccgacaggttttcaataatcggacagtcggcactatcgtcgcccggacacgcgttagccagcgccagcagttggtcccgcatgctctgtaattcttcgatatggcgctcaatttccgcaactttttcaagggtacggcgtttcacatcagcagagtgacgttgcggatcgttgaacaaattgaccagttcaccgctctcctcaaggttgaaccctacttgacgggcctggcgcagtaacgtcaactcattgaggtgctgttgcgtataggtgcgataaccattctctgagcgcatcggcggagtaaccaaacctttttcttcataaaagcggattgctttgctggtcagacctgtgatcttcgccacatcggagatgttcatctgtaatttctcctctttctctagtagctagcacaatacctaggactgagctagctgtcaaactagagagtagatagatagatgcaccccgcctggtttattaatttcttgaccttccccttgctggaaggtttaacctttatcatactagaaagaggagaaatactagatgcaacgtcgtgacttcctgaaatacagtgttgctcttggggttgcttccgcattacccctttggtcccgcgcagtgtttgctggtgaagacccgcacgcaggccataagatgtctcatggtgcgaagtacaaagcacttcttgactcttcctcccattgtgttgcggtaggcgaggactgtctgcgtcactgttttgaaatgcttgctatgaacgacgcgagtatgggggcgtgcaccaaagccacctacgatcttgtagccgcctgcggagcattagctaagctggcggggaccaactcagcctttacaccagccttcgccaaagtcgttgctgatgtatgtgccgcgtgcaaaaaggaatgtgacaagtttccatccattgccgaatgtaaggcttgcggagaggcgtgccaggcttgtgcagaagagtgtcacaaagtcgcggccggcagtgggtctgggtcgagcaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtccgtggagagggtgaaggtgatgctacaaacggaaaactcacccttaaatttatttgcactactggaaaactacctgttccctggccaacacttgtcactactctgacctatggtgttcaatgcttttcccgttatccggatcacatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggacctacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaaaggtattgattttaaagaagatggaaacattctcggacacaaactcgagtacaactttaactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacgttgaagatggttccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgtcctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaacatcatcaccaccatcactaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1321120_sequence/1
type: Sequence
persistent identity: BBa K1321120 sequence
display id: BBa_K1321120_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: accagcaccaccctggttaaatgtgcatgtgaaccgtgtctgtgtaatgttgatccgagcaaagcaattgatcgcaatggtctgtattattgtagcgaagcatgtgcagatggtcatacaggtggtagcaaaggttgtggtcataccggctgtaattgtcatggcaccggccccggcgccaatccgcccggtactacgaccacttcgcgtccagcaaccacgaccgggtcgagccccggcccacaggcctgttcttccgtctggggtcagtgcggcggacagaactggtctggccctacctgctgtgcttctggcagcacatgcgtctactctaacgactactatagccagtgcctgccgggtgctaacccgccaggcactaccactacctctcgccccgctaccactacaggctctagcccgggacctacccaaagccactacggccagtgcggaggcattggctacagcggccctaccgtctgcgcctccggaaccacctgccaagtcctgaacccgtactactcccagtgtctt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1342003_sequence/1
type: Sequence
persistent identity: BBa K1342003 sequence
display id: BBa_K1342003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgacctcaacaacgttggtcaaatgcgcttgtgagccctgtctctgcaacgtcgatcccagcaaagcgatcgatcgcaacggtctgtactactgtagcgaagcctgtgccgatggccacaccggtggtagcaaaggctgcggccacaccggctgtaactgccacggctaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1321009_sequence/1
type: Sequence
persistent identity: BBa K1321009 sequence
display id: BBa_K1321009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcacaccatgaagaacaacacggcggacaccaccaccatcaccatcacaacaccaccaccactaccatggcggcgaacaccaccatcaccaccacagctctcatcatgaagaaggttgttgtagcactagcgatagtcatcatcaagaagaaggttgctgccacgggcatcacgag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1321352_sequence/1
type: Sequence
persistent identity: BBa K1321352 sequence
display id: BBa_K1321352_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggagatactagagaaagaggagaaatactagatggccggcgcacaccatgaagaacaacacggcggacaccaccaccatcaccatcacaacaccaccaccactaccatggcggcgaacaccaccatcaccaccacagctctcatcatgaagaaggttgttgtagcactagcgatagtcatcatcaagaagaaggttgctgccacgggcatcacgagaccggcggtccggccgggtgccaggtgctgtggggcgtcaaccagtggaacaccggcttcaccgcgaacgtcaccgtgaagaacacgtcctccgctccggtagacggctggacgctcacgttcagcttcccgtccggccagcaggtcacccaggcgtggagctcgacggtcacgcagtccggctcggccgtgacggtccgcaacgccccgtggaacggctcgatcccggcgggcggcaccgcgcagttcggcttcaacggctcgcacacgggcaccaacgccgcgccgacggcgttctcgctcaacggcacgccctgcacggtcggcggcagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1321151_sequence/1
type: Sequence
persistent identity: BBa K1321151 sequence
display id: BBa_K1321151_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcaggtacaggttgtaaaatttgggaagattgtaaatgtggtgcagcatgtagctgtggtgatagctgtacctgtggcaccgttaaaaaaggtacaaccagccgtgccggtgcaggttgtccgtgtggtccgaaatgtaaatgtacaggtcagggtagctgtaactgcgttaaagatgattgttgtggttgtggcaaaaccggctcatcaatgtcagttgaattttacaactctaacaaatcagcacaaacaaactcaattacaccaataatcaaaattactaacacgtctgacagtgatttaaatttaaatgacgtaaaagttagatattattacacaagtgatggtacacaaggacaaactttctggtgtgaccatgctggtgcattattaggaaatagctatgttgataacactagcaaagtgacagcaaacttcgttaaagaaacagcaagcccaacatcaacctatgatacatatgttgaatttggatttgcaggcagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1460005_sequence/1
type: Sequence
persistent identity: BBa K1460005 sequence
display id: BBa_K1460005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtactgtgctagctactagagaaagacaggaccctactagatgaatcaccgccaagacgagtttgtgaggtttcaggattggaaatcagagagaagctctgagggaaattttcatgctaaaggtggagtccaccgaagtaaagttgtaatagcttccgacgaattacataatcgcttggaatctggtaaatggagggccaaaggcataatacaagctgtaaaatctagtttaagtggttttgtggaagaaagcttgggatccaaaaagaacattcttgatcctcaaggaccttttcttcgaaagtggaacaaaatatttgtattatcctgtgtaattgctatctccttggatcctttgttcctttatattccagtgattgataacgacaacaaatgcctggggttaaacagaacgttggaggtgacagctagtgttttgcgttcttttactgatatattttactttcttcatattgcccttcaatttcgaactggttttattgctccttcgtcacgtgtatttggaagaggtgttttaattgaagatgcttgggagatagcaaagagatacttatccacttacttcttgatcgacattcttgcagttctaccactaccacaggttgtaattttaattataattcctaagttgcggggttcaagatctttgaataccaagaatttgctgaagtctgttgtctttttccaatatattccaagggtccttcgagtgtatcctttatatagggaagtcacaagaacatctggcatactcactgaaacagcatgggctggagctgctttcaatctcttcctttatatgcttgccagccatgtacttggagccttttggtatctgttttctatagaacgcgaaactacttgctggaaacaagcttgtggaaattcttctccctgtcatcatgcctcattgtattgtgatgatgatcatacaaaatttaaaacattgctgaacagttcctgccctatagagacaccaaatgcaacgctatttgattttgggatattccttggcgctctccaatcaggtgttgtgggaccaatggattttccgcagaagttcttttattgtttctggtggggtttacaaaatttaagttcccttggtcaaaacctccaaacaagtaccttcatctgggaaatgtgctttgcagttttcatttccatcgctggcctggtgctatttgcctttctcattggcaatatgcagacatgtctgcaatcctcaacattaagattagaggagatgagggtgaaaagacgagatgcagaacagtggatgtcacatcggttgctccctgagaacctcagagagcggattaggtgctatgagcaatacaggtggcaggaaactaggggtgttgatgaagagaatctgattcacaaccttccgaaagacctcaggagagatataaagcgtcatctttgtttagctttgctcatgagagtgcccatgtttgaaaaaatggatgaacaactcttagatgcattatgcgaccatctgaagccagtgctattcaccaaggacagcttcattgttcgtgaaggtgatccagttgatgcgatgctgtttgtaatgaggggcaaactactgtctgtaaccactaatggagggaggacaggtttttttaactctgagcatctaaaagctggtgatttttgtggagaggagctacttacttgggctcttgaccctaattcgtcaactaacctaccaatctcaactagaactgcccaagcactctcagaagttgaagcatttgctttagtggctgatgatttaaagcttgtagcctctcagttcagacgacttcacagtaagcagctccgccacactttcaggttttactcaggccaatggagaacttgggcagcttgcttcatacaagcagcatggcggagttattgcaggaagaatgtagaagagtctcttcgtgatgaagaaaacaggttgcaa…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1438002_sequence/1
type: Sequence
persistent identity: BBa K1438002 sequence
display id: BBa_K1438002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgactaccgccagcactagccaggttcgccagaactaccatcaggactccgaggctgcaatcaaccgtcagatcaacctggaactgtacgcgtcttatgtctacctgtctatgagctattacttcgaccgtgatgacgttgcactgaaaaacttcgcgaaatacttcctgcatcagtctcatgaagaacgcgagcacgctgaaaaactgatgaaactgcaaaaccagcgtggtggccgtatttttctgcaagacattaaaaaaccggattgcgacgattgggagtctggtctgaacgccatggaatgcgcactgcacctggagaaaaacgtgaatcaatccctgctggagctgcacaagctggccaccgacaaaaacgatccgcacctgtgtgatttcatcgaaacccactacctgaatgagcaggtgaaagctatcaaagaactgggtgaccacgtaaccaacctgcgtaagatgggtgcgccggaatccggcctggcggaatatctgtttgacaaacacacgctgggcgattccgataacgaatctatgagctcccagatccgtcagaactacagcaccgacgtagaagcagctgtgaactccctggtcaatctgtacctgcaagccagctatacgtacctgtctctgggtttctatttcgatcgtgacgatgtggcgctggaaggcgtttctcacttcttccgcgaactggctgaagaaaaacgtgagggttacgaacgtctgctgaaaatgcagaaccaacgtggcggtcgtgccctgttccaggacattaagaaaccggcggaagatgagtggggtaaaactccggatgctatgaaagcagcgatggcactggagaagaaactgaaccaggcgctgctggacctgcatgctctgggttctgctcgcactgacccgcacctgtgcgattttctggagacccacttcctggacgaagaagttaaactgatcaagaaaatgggcgaccacctgaccaacctgcaccgtctgggcggtccagaagcgggcctgggtgaatacctgtttgagcgcctgaccctgaaacatgattaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1701000_sequence/1
type: Sequence
persistent identity: BBa K1701000 sequence
display id: BBa_K1701000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcagttccatattgatgacatgacctgcggcggctgcgccagtacggtaaaaaagacgattctgactctcgatgctaatgcgacggtgagaactgacccggcgacgcgtctggttgacgttgaaacgtcgctatccgcggagcagattgccgccgccctgcaaaaggccggtttcccgccgcgcgagaggtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1933102_sequence/1
type: Sequence
persistent identity: BBa K1933102 sequence
display id: BBa_K1933102_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtactatgctagcactagagaaagaggagaaatactagatggcgttcgacccgaacctggttggtccgaccctgccgccgatcccgccgttcaccctgccgacccatcatcatcatcatcatggtccggccgggtgccaggtgctgtggggcgtcaaccagtggaacaccggcttcaccgcgaacgtcaccgtgaagaacacgtcctccgctccggtagacggctggacgctcacgttcagcttcccgtccggccagcaggtcacccaggcgtggagctcgacggtcacgcagtccggctcggccgtgacggtccgcaacgccccgtggaacggctcgatcccggcgggcggcaccgcgcagttcggcttcaacggctcgcacacgggcaccaacgccgcgccgacggcgttctcgctcaacggcacgccctgcacggtcggcggcagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1321349_sequence/1
type: Sequence
persistent identity: BBa K1321349 sequence
display id: BBa_K1321349_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggtccggccgggtgccaggtgctgtggggcgtcaaccagtggaacaccggcttcaccgcgaacgtcaccgtgaagaacacgtcctccgctccggtagacggctggacgctcacgttcagcttcccgtccggccagcaggtcacccaggcgtggagctcgacggtcacgcagtccggctcggccgtgacggtccgcaacgccccgtggaacggctcgatcccggcgggcggcaccgcgcagttcggcttcaacggctcgcacacgggcaccaacgccgcgccgacggcgttctcgctcaacggcacgccctgcacggtcggcggcagcaccggccgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1319106_sequence/1
type: Sequence
persistent identity: BBa K1319106 sequence
display id: BBa_K1319106_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagatggccggc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1933011_sequence/1
type: Sequence
persistent identity: BBa K1933011 sequence
display id: BBa_K1933011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaccctggacaaagctctggttctgcgtacctgcgctaacaacatggctgaccactgcggtctgatctggccggcttctggtaccgttgaatctcgttactggcagtctacccgtcgtcacgaaaacggtctggttggtctgctgtggggtgctggtacctctgctttcctgtctgttcacgctgacgctcgttggatcgtttgcgaagttgctgttgctgacatcatctctctggaagaaccgggtatggttaaattcccgcgtgctgaagttgttcacgttggtgaccgtatctctgcttctcacttcatctctgctcgtcaggctgacccggcttctacctctacctctacctctacctctaccctgaccccgatgccgaccgctatcccgaccccgatgccggctgttgcttctgttaccctgccggttgctgaacaggctcgtcacgaagttttcgacgttgcttctgtttctgctgctgctgctccggttaacaccctgccggttaccaccccgcagaacctgcaaaccgctacctacggttctaccctgtctggtgacaaccactctcgtctgatcgctggttacggttctaacgaaaccgctggtaaccactctgacctgatcggtggtcacgactgcaccctgatggctggtgaccagtctcgtctgaccgctggtaaaaactctgttctgaccgctggtgctcgttctaaactgatcggttctgaaggttctaccctgtctgctggtgaagactctaccctgatcttccgtctgtgggacggtaaacgttaccgtcagctggttgctcgtaccggtgaaaacggtgttgaagctgacatcccgtactacgttaacgaagacgacgacatcgttgacaaaccggacgaagacgacgactggatcgaagttaaaggtggcggtagtggcggtgcaggaggcggatcccatcatcatcatcatcat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1404002_sequence/1
type: Sequence
persistent identity: BBa K1404002 sequence
display id: BBa_K1404002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aactgttaattagtaggccgagcatattacacaccttaacactcgcctattgttaaagtgtgtcctttgtctggtactagagaaggaggtatataatgggtggtgcggcggcgcgcctgcgccgccatccgagctttgtttatagcagcattgcggaaccgattatgcatctgaccaaaagcctgccgagcctgagcctgcgccgccgcattggtgcgctgtttgcgggtctgattgcggcgaacattggtgtttgggtttgggcgtttagcctgtttcatgcgcagccgctgatgctgggtaccgcggttctggcgtggggtctgggtctgcgccatgcggttgatgcggatcatattgcggcgattgataacgttacccgcaaactgatgcaggatggtcagcgcccggttagcgttggtttttggtttgcgattggtcatagcggtattattgcgattgcgagcattattattgcggttaccgcgagcgcgctgagccagtttggtgcgtttaaagaaattggtggtgttattgcgaccgttattagcgcgctgtttctgtttaccattgcgggtatgaacctggttattctgcgcagcgtttggcgcacctttgcgcatgttcgcgcgggtggtagctatgcggaagatgatctggatctgctgctgggtggtcgcggtctgctgagccgcctgtttcgcccgatgtttcgcctggttaacaaaagctggcatatggcgccgctgggttttctgtttggtctgggttttgataccgcgaccgaagttgcgattctgggtatgagcgcgagccaggcggcggatggtctgagcattggtaccattctggttctgccggcgctgtttgcggttggtatggcgctgattgataccgcggatggtgttgttatgctgggtgcgtatgaatgggcgtttgttaaaccgattcgcaaactgtattataacattaccattaccctgattagcgcgattgttgcgattgttattggtggtattgaaaccctggcgctgattggtgataaactggcgctgaccggtgcgccgtggcgcattgcgattgaactgggtgaaaactttaacggtctgggttttgcgattattggtctgtttgttttttgctggctggcgagctatgcgatttatcgctggaaacgctttgatgaaattgaagttagcgttggtgcgtgatcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K863103_sequence/1
type: Sequence
persistent identity: BBa K863103 sequence
display id: BBa_K863103_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggaaagaggagaaataatgggtccggccgggtgccaggtgctgtggggcgtcaaccagtggaacaccggcttcaccgcgaacgtcaccgtgaagaacacgtcctccgctccggtagacggctggacgctcacgttcagcttcccgtccggccagcaggtcacccaggcgtggagctcgacggtcacgcagtccggctcggccgtgacggtccgcaacgccccgtggaacggctcgatcccggcgggcggcaccgcgcagttcggcttcaacggctcgcacacgggcaccaacgccgcgccgacggcgttctcgctcaacggcacgccctgcacggtcggcggcagcaccggcatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaacatcaccatcaccatcacaccggttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I721002_sequence/1
type: Sequence
persistent identity: BBa I721002 sequence
display id: BBa_I721002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgatgcggatcggtgaactgggcaagaaggcagattgcttggtgcagaccgtgcgcttttacgagtcagaaggcttgctgcccgagcctgcacgtagcgagggcaacttcaggctctatgacgaagtccatttgcagcgcttgctgttcatccgccgctgccgggcgaaggacatgacgctggatgagatccgtcaactgctgaacttacgggatcggccagagttgggctgcggcgaggtgaacgcgctggtcgacgctcatatcgcgcaagtgcggaccaagatgaaggaattgcgcgccttggagcgcgagttaatggatctgcgacgctcctgcgatagcgcccgaacctcgcgcgagtgcggcattctcaacagcttggccgagcccgcctga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1438000_sequence/1
type: Sequence
persistent identity: BBa K1438000 sequence
display id: BBa_K1438000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagaggatcgcatcaccatcaccatcacatgggatccaaaggtgatactaaagttataaattatctcaacaaactgttgggaaatgagcttgtcgcaatcaatcagtactttcttcatgcccggatgtttaaaaactggggtctcaaacgtctcaatgatgtggagtatcatgaatccattgatgagatgaaacacgccgatcgttatattgagcgcattctttttctggaaggtcttccaaacttacaggacctgggcaaactgaacattggtgaagatgttgaggaaatgctgcgttctgatctggcacttgagctggatggcgcgaagaatttgcgtgaggcaattggttatgccgatagcgttcatgattacgtcagccgcgatatgatgatagaaattttgcgcgatgaagaaggccatatcgactggctggaaacggaacttgatctgattcagaagatgggactgcaaaattatctgcaagcacagattcgcgaagaaggttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1321348_sequence/1
type: Sequence
persistent identity: BBa K1321348 sequence
display id: BBa_K1321348_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaaaccggccccggcgccaatccgcccggtactacgaccacttcgcgtccagcaaccacgaccgggtcgagccccggcccacaggcctgttcttccgtctggggtcagtgcggcggacagaactggtctggccctacctgctgtgcttctggcagcacatgcgtctactctaacgactactatagccagtgcctgccgggtgctaacccgccaggcactaccactacctctcgccccgctaccactacaggctctagcccgggacctacccaaagccactacggccagtgcggaggcattggctacagcggccctaccgtctgcgcctccggaaccacctgccaagtcctgaacccgtactactcccagtgtctt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1420002_sequence/1
type: Sequence
persistent identity: BBa K1420002 sequence
display id: BBa_K1420002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaagctcgccccatatattttagaacgtctcacttcggtcaatcgtaccaatggtactgcggatctcttggtcccgctactgcgggaactcgccaaggggcgtccggtttcacgaacgacacttgccgggattctcgactggcccgctgagcgagtggccgccgtactcgaacaggccaccagtaccgaatatgacaaagatgggaacatcatcggctacggcctcaccttgcgcgagacttcgtatgtctttgaaattgacgaccgccgtctgtatgcctggtgcgcgctggacaccttgatatttccggcgctgatcggccgtacagctcgcgtctcatcgcattgcgctgcaaccggagcacccgtttcactcacggtttcacccagcgagatacaggctgtcgaacctgccggcatggcggtgtccttggtattgccgcaggaagcagccgacgttcgtcagtccttctgttgccatgtacatttctttgcatctgtcccgacggcggaagactgggcctccaagcatcaaggattggaaggattggcgatcgtcagtgtccacgaggctttcggcttgggccaggagtttaatcgacatctgttgcagaccatgtcatctaggacaccgtga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K190021_sequence/1
type: Sequence
persistent identity: BBa K190021 sequence
display id: BBa_K190021_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgacctcaacaacgttggtcaaatgcgcttgtgagccctgtctctgcaacgtcgatcccagcaaagcgatcgatcgcaacggtctgtactactgcagcgaagcctgtgccgatggccacaccggtggtagcaaaggctgcggccacaccggctgtaactgccacggctaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J04500_sequence/1
type: Sequence
persistent identity: BBa J04500 sequence
display id: BBa_J04500_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K160001_sequence/1
type: Sequence
persistent identity: BBa K160001 sequence
display id: BBa_K160001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcaccttccaaaactgctcagccaaaacactcactgcgtaaaatcgcagttgtagtagccacagcggttagcggcatgtctgtttatgcacaggcagcggttgaaccgaaagaagacactatcaccgttaccgctgcacctgcgccgcaagaaagcgcatgggggccggctgcaactattgcggcgcgacagtcagctaccggcactaaaaccgatacgccgattcaaaaagtacctcaatctatttctgtcgtcaccgctgaagagatggcgctgcatcagcctaagtcggtgaaagaagcgcttagctatacgccgggtgtctctgttggtacgcgtggcgcatccaacacctatgaccacctgatcattcgcggttttgcggcagaaggccaaagccagaataactatctgaatggcctgaagttgcagggcaacttctataacgatgcggtcatcgacccgtatatgctggaacgtgctgaaattatgcgtggcccggtttccgtgctttacggtaaaagcagtcctggcggtctgttgaatatggtcagcaagcgtccgaccaccgaaccgctgaaagaagttcagtttaaagccggtactgacagcctgttccagaccggttttgacttcagcgatgcgctggatgatgacggcgtttattcttatcgcctgaccggtcttgcgcgttctgccaatgcccagcagaaagggtcagaagagcagcgttatgctattgcaccggcgttcacctggcgtccggatgataaaaccaatttcaccttcctttcttacttccagaacgagccggaaactggttattacggctggttgccgaaagagggaaccgttgagccgctgccgaacggtaagcgtctgccgacagactttaacgaaggggcgaagaacaacacctattctcgtaacgagaagatggtcggctacagcttcgatcacgaatttaacgatacctttactgtgcgtcagaacctgcgctttgctgaaaacaaaacctcgcaaaacagcgtttatggttacggcgtctgctccgatccggcgaatgcttacagcaaacagtgtgcggcattagcgccagcggataaaggccattatctggcacgtaaatacgtcgttgatgatgagaagctgcaaaacttctccgttgatacccagttgcagagcaagtttgccactggcgatatcgaccacactctgctgaccggtgtcgactttatgcgtatgcgtaatgacatcaacgcctggtttggttacgacgactctgtgccactgctcgatctgtacaatccggtgaataccgattttgacttcaatgcgaaagacccggataactccgggccgtaccgcattctgaataagcagaagcaaacgggcgtttatgttcaggatcaggcgcagtgggataaagtgctggtcaccctgggcggtcgttacgactgggcagatcaagaatctcttaaccgcgtagcgggaacgaccgataaacgtgatgacaaacagtttacctggcgtggtggtgttaactacctgtttgataatggtgtaacaccatacttcagctatagcgaatcgtttgaaccttcttcgcaagttgggaaggatggtaatattttcgcaccgtctaaaggtaagcagtatgaagtcggcgtgaaatatgtaccggaagatcgtccgattgtagttactggtgccgtgtataatcttactaaaaccaacaacctgatggcggaccctgagggttccttcttctcggttgaaggtggcgagatccgcgctcgtggcgtagaaatcgaagcgaaagcggcgctgtcggcgagtgttaacgtagtcggttcttatacttacaccgatgcggaatacactaccgatactacctacaaaggcaatacgcctgcacaggtgccaaaacacatggcttcgctgtgggctgactataccttctttgacggtccgctttcaggtctgacgctgggcac…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1321100_sequence/1
type: Sequence
persistent identity: BBa K1321100 sequence
display id: BBa_K1321100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggagatactagagaaagaggagaaatactagatggccggcgaatgtgaatgtgagtgcgaatgcgagtgtgaatgtgagtgtgagtgtgaatgtgaatgcgagtgtgagtgcgagtgcgaatgtgagtgtgaatgcgagtgcgaatgcgaatgcgagtgcggcaccggccccggcgccaatccgcccggtactacgaccacttcgcgtccagcaaccacgaccgggtcgagccccggcccacaggcctgttcttccgtctggggtcagtgcggcggacagaactggtctggccctacctgctgtgcttctggcagcacatgcgtctactctaacgactactatagccagtgcctgccgggtgctaacccgccaggcactaccactacctctcgccccgctaccactacaggctctagcccgggacctacccaaagccactacggccagtgcggaggcattggctacagcggccctaccgtctgcgcctccggaaccacctgccaagtcctgaacccgtactactcccagtgtctt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1694006_sequence/1
type: Sequence
persistent identity: BBa K1694006 sequence
display id: BBa_K1694006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcacggtaagacccaagccaccagtggcaccatccagagcatgcatggtaaaacccaggcaaccagcggcaccatccaaagcatgcatggcaaaacccaggccaccagtggtaccattcagagctaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1321003_sequence/1
type: Sequence
persistent identity: BBa K1321003 sequence
display id: BBa_K1321003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggtccggccgggtgccaggtgctgtggggcgtcaaccagtggaacaccggcttcaccgcgaacgtcaccgtgaagaacacgtcctccgctccggtagacggctggacgctcacgttcagcttcccgtccggccagcaggtcacccaggcgtggagctcgacggtcacgcagtccggctcggccgtgacggtccgcaacgccccgtggaacggctcgatcccggcgggcggcaccgcgcagttcggcttcaacggctcgcacacgggcaccaacgccgcgccgacggcgttctcgctcaacggcacgccctgcacggtcggcggcagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1321356_sequence/1
type: Sequence
persistent identity: BBa K1321356 sequence
display id: BBa_K1321356_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggccggccgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaaaccggctcatcaatgtcagttgaattttacaactctaacaaatcagcacaaacaaactcaattacaccaataatcaaaattactaacacgtctgacagtgatttaaatttaaatgacgtaaaagttagatattattacacaagtgatggtacacaaggacaaactttctggtgtgaccatgctggtgcattattaggaaatagctatgttgataacactagcaaagtgacagcaaacttcgttaaagaaacagcaagcccaacatcaacctatgatacatatgttgaatttggatttgcaggcagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1980006_sequence/1
type: Sequence
persistent identity: BBa K1980006 sequence
display id: BBa_K1980006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttattaccctgcgcgatgatgacaacaacccgacaggttttcaataatcggacagtcggcactatcgtcgcccggacacgcgttagccagcgccagcagttggtcccgcatgctctgtaattcttcgatatggcgctcaatttccgcaactttttcaagggtacggcgtttcacatcagcagagtgacgttgcggatcgttgaacaaattgaccagttcaccgctctcctcaaggttgaaccctacttgacgggcctggcgcagtaacgtcaactcattgaggtgctgttgcgtataggtgcgataaccattctctgagcgcatcggcggagtaaccaaacctttttcttcataaaagcggattgctttgctggtcagacctgtgatcttcgccacatcggagatgttcatctgtaatttctcctctttctctagtagctagcacaatacctaggactgagctagctgtcaaactagagagtagatagatagatgcaccccgcctggtttattaatttcttgaccttccccttgctggaaggtttaacctttatcatactagaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1321110_sequence/1
type: Sequence
persistent identity: BBa K1321110 sequence
display id: BBa_K1321110_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggccggcgaatgtgaatgtgagtgcgaatgcgagtgtgaatgtgagtgtgagtgtgaatgtgaatgcgagtgtgagtgcgagtgcgaatgtgagtgtgaatgcgagtgcgaatgcgaatgcgagtgcggcaccggccccggcgccaatccgcccggtactacgaccacttcgcgtccagcaaccacgaccgggtcgagccccggcccacaggcctgttcttccgtctggggtcagtgcggcggacagaactggtctggccctacctgctgtgcttctggcagcacatgcgtctactctaacgactactatagccagtgcctgccgggtgctaacccgccaggcactaccactacctctcgccccgctaccactacaggctctagcccgggacctacccaaagccactacggccagtgcggaggcattggctacagcggccctaccgtctgcgcctccggaaccacctgccaagtcctgaacccgtactactcccagtgtctt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1321338_sequence/1
type: Sequence
persistent identity: BBa K1321338 sequence
display id: BBa_K1321338_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggagatactagagaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1980002_sequence/1
type: Sequence
persistent identity: BBa K1980002 sequence
display id: BBa_K1980002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtgtaattcgcatgactaattatgaagccggtacacttttgacttgtagccacgaggggtgcggctgtcgtgttcgtattgaagtcccgtgtcattgtgccggagcgggcgacgcttaccgctgcacctgtggagatgaactggctcctgtgaaacatcatcaccaccatcactaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K346004_sequence/1
type: Sequence
persistent identity: BBa K346004 sequence
display id: BBa_K346004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgcaggattgcggtgaagtcaatatgctcttggatgagcacatccgtcaggtcgaatctcggatcggagctttgctcgaactgaagcaccatttggtggaactgcgcgaagcctgttctggtgccaggcccgcccaatcgtgcgggattctgcacggactgtcggactcttccggacaggattgcggtgaagtcaatatgctcttggatgagcacatccgtcaggtcgaatctcggatcggagctttgctcgaactgaagcaccatttggtggaactgcgcgaagcctgttctggtgccaggcccgcccaatcgtgcgggtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1321093_sequence/1
type: Sequence
persistent identity: BBa K1321093 sequence
display id: BBa_K1321093_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaatgtgaatgtgagtgcgaatgcgagtgtgaatgtgagtgtgagtgtgaatgtgaatgcgagtgtgagtgcgagtgcgaatgtgagtgtgaatgcgagtgcgaatgcgaatgcgagtgcggcaccggcaatgctacgccaactaagggtgcaaccccgaccaacacagcaacgcctacaaaaagcgctacagcaacacctacaagaccgtcagttcctacaaacacaccgactaacacaccggcaaatacacctgtttcaggcaacttgaaggtcgaattctataactcaaatccgagtgatacaactaacagtattaatccgcagtttaaagtaacaaatacaggatcaagtgcaattgatctttcaaagcttacattgagatactattacaccgttgatggccagaaggaccagactttctggtgtgaccatgcagctatcataggtagcaacggctcatacaacggcatcacatcaaatgtaaaaggaacattcgtaaagatgagctcaagcacaaataacgcagacacatacctcgaaataagtttcacaggtggcactttggaacctggtgctcatgtacagatacagggtaggtttgcgaaaaatgactggagtaattatacacagtcaaatgattactcatttaagtcagcatcacagttcgtagaatgggatcaggttacagcatatttgaatggagtacttgtatggggtaaagaaccaggaggatcagtagttccgtcaacacagccggtaacaaccccaccggcaacaaccaagccgccagcaacaaccaaaccaccggctaccacgattcctccatcagacgatccg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1122666_sequence/1
type: Sequence
persistent identity: BBa K1122666 sequence
display id: BBa_K1122666_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggaaaaccgtattgatcgtattaagaaacaactgcactcatccagctataaacttacgccacagcgtgaagcgacagtaagagtgctgcttgaaaacgaagaagaccatttaagcgcagaagatgtatacctcctcgtaaaagagaaatctcctgagatcggtctcgctacagtataccgtacgcttgaattattaactgaattaaaagtcgtagataaaattaactttggagacggtgtgtcccgttacgaccttcggaaagagggcgcagctcactttcatcaccacttggtgtgcatggagttcggagccgttgatgaaattgaaggagatttgcttgaagacgtggaagaaatcattgaacgtgattggaaatttaagattaaagatcatagattgacgtttcacggcatttgccaccgctgtaacggaaaagaaactgaatag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1122702_sequence/1
type: Sequence
persistent identity: BBa K1122702 sequence
display id: BBa_K1122702_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaacatctatccgatacgcactgcttgccgcagccctgaccgccgccacccccgcgctggcagacattaccgtgtacaacggccaacacaaagaagcggcacaagccgttgcagatgcctttacccgggctaccggcatcaaagtcaaactcaacagtgccaaaggcgaccagcttgccggccaaatcaaagaagaaggcagccgaagccccgccgacgtattctattccgaacaaatcccggcactcgccaccctttccgcagccaacctcctagagcccctgcccgcctccaccatcaacgaaacacgcggcaaaggcgtgccggttgccgccaaaaaagactgggtggcactgagcggacgttcgcgcgtcgtcgtttacgacacccgcaaactgtctgaaaaagatttggaaaaatccgtcctgaattacgccacgccgaaatggaaaaaccgcatcggttacgtccccacttccggcgcgttcttggaacagattgtcgccatcgtcaaactgaaaggcgaagcggccgcattgaaatggctcaaaggcctgaaagaatacggcaagccttacgctaaaaactccgtcgcccttcaagcggttgaaaacggcgaaatcgatgccgccctcatcaacaactactactggcacgctttcgcgcgtgaaaaaggcgtacaaaatgtccacacccgcctgaatttcgtccgccacagagatcccggcgcactcgttacctattccggcgcagccgtgttaaaatcctcccaaaacaaggatgaggcgaaaaaattcgtcgccttcctcgccggcaaggaaggacagcgcgccctgaccgccgtccgtgccgaatatcctttgaatccgcacgtggtatccaccttcaatttggaacccatcgccaagttggaagcaccccaagtgtccgccaccactgtttccgaaaaagaacacgccacccggctgcttgagcaagccggtatgaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1460001_sequence/1
type: Sequence
persistent identity: BBa K1460001 sequence
display id: BBa_K1460001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtaccgagctcgtaatacgactcactatagggaatacaagctacttgttctttttgcaggtacctactagagaaagacaggaccctactagatgtcccctatactaggttattggaaaattaagggccttgtgcaacccactcgacttcttttggaatatcttgaagaaaaatatgaagagcatttgtatgagcgcgatgaaggtgataaatggcgaaacaaaaagtttgaattgggtttggagtttcccaatcttccttattatattgatggtgatgttaaattaacacagtctatggccatcatacgttatatagctgacaagcacaacatgttgggtggttgtccaaaagagcgtgcagagatttcaatgcttgaaggagcggttttggatattagatacggtgtttcgagaattgcatatagtaaagactttgaaactctcaaagttgattttcttagcaagctacctgaaatgctgaaaatgttcgaagatcgtttatgtcataaaacatatttaaatggtgatcatgtaacccatcctgacttcatgttgtatgacgctcttgatgttgttttatacatggacccaatgtgcctggatgcgttcccaaaattagtttgttttaaaaaacgtattgaagctatcccacaaattgataagtacttgaaatccagcaagtatatagcatggcctttgcagggctggcaagccacgtttggtggtggcgaccatcctccaaaatcggatctggttccgcgtggatccattcatatgatgactgtaaaaatatgtgactgtgaaggcgaatgttgtaaggactcttgtcattgtgggagcacctgccttccaagctgttctggcggtgaaaagtgcaaatgtgatcacagcaccggaagccctcaatgtaagagttgtggtgaaaaatgcaaatgcgaaaccacgtgcacttgtgaaaagagtaaatgcaattgtgaaaaatgttagaaaaaaaaaccccgcccctgacagggcggggttttttttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1321337_sequence/1
type: Sequence
persistent identity: BBa K1321337 sequence
display id: BBa_K1321337_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1499004_sequence/1
type: Sequence
persistent identity: BBa K1499004 sequence
display id: BBa_K1499004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggcgatcgcatgagcagcatgtcggtggagttctataatagcaacaagtcagcacagaccaattcaattacgccaattatcaaaatcaccaatacaagtgatagcgacttgaacttaaacgacgtcaaagttcgctattactacactagcgacgggactcagggtcagacgttttggtgtgatcacgctggagccctgctgggcaatagctacgtggataatacctccaaagtaaccgccaactttgtgaaagaaacggcgagcccgacctcgacgtacgatacctacgtcgaatttggcttcgccggcggcggcggtagtggcggtgcagaagctgggattacaggtacgtggtataatcagttaggttcgacattcatcgtaacagctggtgccgacggtgcgctgactggcacgtatgaatcagcggtcggcaacgcggaaagtcgctacgtgctgacggggcgctacgatagcgcacctgctactgatggctcgggcacagctttgggctggaccgtagcatggaaaaacaactatcgcaacgcgcacagcgcgacgacctggtctggccaatatgtgggcggtgccgaggctcgcatcaacacgcagtggctgctcactagcggaacaacagaagcgaatgcatggaagagcaccctggtcggccatgacacgtttaccaaagtaaaaccctccgccgctagcggtagcggtggtagcggtggctcactgtcttcaccgaccattcaaatgtataactcaaacaaagagatcgaaactaatacgatctcgcccacatttaaaatcatcaatagctcttatagccccctggatctcaaagacgttactgtacgttactattatacctcggatggcaatcaggagcagaatttctggtgtgaccatgccgatgcattgctgggctataactatgtcgataatacttctaaagttactggtaaatttgtaaaattcccgaacggcattggaaactgcgatacttatctggaaattggcttccataaccaccgccacaaacatgtttaaac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1933004_sequence/1
type: Sequence
persistent identity: BBa K1933004 sequence
display id: BBa_K1933004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtactatgctagcactagagaaagaggagaaatactag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1321121_sequence/1
type: Sequence
persistent identity: BBa K1321121 sequence
display id: BBa_K1321121_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: accagcaccaccctggttaaatgtgcatgtgaaccgtgtctgtgtaatgttgatccgagcaaagcaattgatcgcaatggtctgtattattgtagcgaagcatgtgcagatggtcatacaggtggtagcaaaggttgtggtcataccggctgtaattgtcatggcaccggctcatcaatgtcagttgaattttacaactctaacaaatcagcacaaacaaactcaattacaccaataatcaaaattactaacacgtctgacagtgatttaaatttaaatgacgtaaaagttagatattattacacaagtgatggtacacaaggacaaactttctggtgtgaccatgctggtgcattattaggaaatagctatgttgataacactagcaaagtgacagcaaacttcgttaaagaaacagcaagcccaacatcaacctatgatacatatgttgaatttggatttgcaggcagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2127002_sequence/1
type: Sequence
persistent identity: BBa K2127002 sequence
display id: BBa_K2127002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaattaaagaggagaaaatgggactaccttggtatcgcgttcatacagttgtcctgaatgatccagggcgactcatctctgtccatttaatgcacactgccctagtggctggctgggctggatctatggctctctatgagttggccatttttgactccagcgatgctgtgcttaaccccatgtggcggcaaggcatgtttgttttgcccttcatggcccgcctcggtgtcaccagttcctggaatggctggagcgtcaccggagaaactggtttggatcccggtttctggtcctttgaaggggtagctgctgcccacatcgttctatctggtctgttgttcctagccgccgtatggcactgggtattttgggacctggaattatttgttgacccccgtaccggtgaatccgcattggatttgcccaaaatgtttggtattcacctcttcttgtctggtttgctctgcttcggttttggtgctttccacctcaccggcgtttggggccctggcatgtgggtatccgacccctatggtctgacgggccacgtacagccagtggcaccggaatggggaccagctggctttaacccgtttaaccccggtggggtagtggctcaccacattgctgctggtattgtcggtatcattgccggtcttttccaccttacggtacggccccctgagcgcttgtataaagccctccgcatggggaatattgagaccgtattgtccagtagtattgccgctgtattttttgccgctttcgtcgtggccggtaccatgtggtacggtaatgccaccacccccatcgaactctttggccccacccgttatcagtgggataagggttattttcaagaagaaatccaacgccgggttgatagccagttagcagaaggagcttccctatctgaagcctggtccaccatccctgaaaagttggctttctacgattacgtcggtaacagccccgctaaaggtggtttgtttcgtaccggtgctatgaacagtggtgatggcattgcccaggaatggattggtcaccccatattcaaagacaaagaaggtcgggaactggaggtacggcgtatgcctaacttctttgaaactttccccgtcatcatgaccgatgcggatggtgtagtccgggcggatattcccttccgtcgttccgagtctaaattcagtgtggaacaaaccggtgttaccgtcagcttctacggtggtgctttagacggccagaccttcagcaatcccagtgatgtgaagaagtttgcccggaaagctcagttgggtgaaggcttcgacttcgatacggaaaccttcaactctgatggtgtattccgcaccagtccccggggttggtttacctttggccacgctgtctttgccttgctcttcttctttggtcacatctggcatggttcccggactctgttccgtgacgtatttgctggggttgatcctggtttggaagaacaagtggaatttggtgtgtttgctaaggttggtgacctttccacccggaaagaagcccaccatcaccatcaccatgtgtagtagcttccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1321091_sequence/1
type: Sequence
persistent identity: BBa K1321091 sequence
display id: BBa_K1321091_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggccggcgaatgtgaatgtgagtgcgaatgcgagtgtgaatgtgagtgtgagtgtgaatgtgaatgcgagtgtgagtgcgagtgcgaatgtgagtgtgaatgcgagtgcgaatgcgaatgcgagtgcggcaccggttaaaccggctcatcaatgtcagttgaattttacaactctaacaaatcagcacaaacaaactcaattacaccaataatcaaaattactaacacgtctgacagtgatttaaatttaaatgacgtaaaagttagatattattacacaagtgatggtacacaaggacaaactttctggtgtgaccatgctggtgcattattaggaaatagctatgttgataacactagcaaagtgacagcaaacttcgttaaagaaacagcaagcccaacatcaacctatgatacatatgttgaatttggatttgcaggcagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1321115_sequence/1
type: Sequence
persistent identity: BBa K1321115 sequence
display id: BBa_K1321115_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggccggcggtccggccgggtgccaggtgctgtggggcgtcaaccagtggaacaccggcttcaccgcgaacgtcaccgtgaagaacacgtcctccgctccggtagacggctggacgctcacgttcagcttcccgtccggccagcaggtcacccaggcgtggagctcgacggtcacgcagtccggctcggccgtgacggtccgcaacgccccgtggaacggctcgatcccggcgggcggcaccgcgcagttcggcttcaacggctcgcacacgggcaccaacgccgcgccgacggcgttctcgctcaacggcacgccctgcacggtcggcggcagcaccggcgaatgtgaatgtgagtgcgaatgcgagtgtgaatgtgagtgtgagtgtgaatgtgaatgcgagtgtgagtgcgagtgcgaatgtgagtgtgaatgcgagtgcgaatgcgaatgcgagtgcggc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1471000_sequence/1
type: Sequence
persistent identity: BBa K1471000 sequence
display id: BBa_K1471000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgggccaagtccgctattttctcacgcacatcgcttagtctatgctctgcaagactactcgcgtccagtcagtgggttccgtcttcctccaggaatagctcggcgatctcgtcacgcttaaatcctagtaggtgcgcagactttacaaactttacccggacaacatcagcttctccc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1321114_sequence/1
type: Sequence
persistent identity: BBa K1321114 sequence
display id: BBa_K1321114_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggccggctcatcaatgtcagttgaattttacaactctaacaaatcagcacaaacaaactcaattacaccaataatcaaaattactaacacgtctgacagtgatttaaatttaaatgacgtaaaagttagatattattacacaagtgatggtacacaaggacaaactttctggtgtgaccatgctggtgcattattaggaaatagctatgttgataacactagcaaagtgacagcaaacttcgttaaagaaacagcaagcccaacatcaacctatgatacatatgttgaatttggatttgcaggcagcaccggcgaatgtgaatgtgagtgcgaatgcgagtgtgaatgtgagtgtgagtgtgaatgtgaatgcgagtgtgagtgcgagtgcgaatgtgagtgtgaatgcgagtgcgaatgcgaatgcgagtgcggc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1979001_sequence/1
type: Sequence
persistent identity: BBa K1979001 sequence
display id: BBa_K1979001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggtcaggcttgctcttctgtttggggtcagtgcggtggtcagaactggtctggtccgacctgctgcgcttctggttctacctgcgtttactctaacgactactactctcagtgcctgccgggtgctgcttcttcttcttctggttct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1420005_sequence/1
type: Sequence
persistent identity: BBa K1420005 sequence
display id: BBa_K1420005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtctgaacctcaaaacgggcgcggggcgctcttcactggcgggctagccgccatcctcgcctcggcttgctgcctggggccgctggttctgatcgccctggggttcagcggcgcttggatcggcaacttgacggtgttggaaccttatcgcccgatcttcatcggcgcggcgttggtggcgctgtttttcgcctggcggcgcatctaccgaccggcgcaagcctgcaaaccaggggatgtgtgtgcgattccccaagtgcgcgctacttacaagctcattttctgggtcgtggccgcgctggttctggtcgcgctcggatttccctacgtcatgccatttttctattaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K863104_sequence/1
type: Sequence
persistent identity: BBa K863104 sequence
display id: BBa_K863104_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagcttctagagaaagacaggacccactagatggccggcggtccggccgggtgccaggtgctgtggggcgtcaaccagtggaacaccggcttcaccgcgaacgtcaccgtgaagaacacgtcctccgctccggtagacggctggacgctcacgttcagcttcccgtccggccagcaggtcacccaggcgtggagctcgacggtcacgcagtccggctcggccgtgacggtccgcaacgccccgtggaacggctcgatcccggcgggcggcaccgcgcagttcggcttcaacggctcgcacacgggcaccaacgccgcgccgacggcgttctcgctcaacggcacgccctgcacggtcggcggcagcaccggcatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaacatcaccatcaccatcacaccggttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K205004_sequence/1
type: Sequence
persistent identity: BBa K205004 sequence
display id: BBa_K205004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtctgaacctcaaaacgggcgcggggcgctcttcactggcgggctagccgccatcctcgcctcggcttgctgcctggggccgctggttctgatcgccctggggttcagcggcgcttggatcggcaacttgacggtgttggaaccttatcgcccgatcttcatcggcgcggcgttggtggcgctgtttttcgcctggcggcgcatctaccgaccggcgcaagcctgcaaaccaggggatgtgtgtgcgattccccaagtgcgcgctacttacaagctcattttctgggtcgtggccgcgctggttctggtcgcgctcggatttccctacgtcatgccatttttctattaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K519010_sequence/1
type: Sequence
persistent identity: BBa K519010 sequence
display id: BBa_K519010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgacctcaacaacgttggtcaaatgcgcttgtgagccctgtctctgcaacgtcgatcccagcaaagcgatcgatcgcaacggtctgtactactgcagcgaagcctgtgccgatggccacaccggtggtagcaaaggctgcggccacaccggctgtaactgccacggctaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1321097_sequence/1
type: Sequence
persistent identity: BBa K1321097 sequence
display id: BBa_K1321097_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: cccggcgccaatccgcccggtactacgaccacttcgcgtccagcaaccacgaccgggtcgagccccggcccacaggcctgttcttccgtctggggtcagtgcggcggacagaactggtctggccctacctgctgtgcttctggcagcacatgcgtctactctaacgactactatagccagtgcctgccgggtgctaacccgccaggcactaccactacctctcgccccgctaccactacaggctctagcccgggacctacccaaagccactacggccagtgcggaggcattggctacagcggccctaccgtctgcgcctccggaaccacctgccaagtcctgaacccgtactactcccagtgtctttactagagaccggctactagaggaatgtgaatgtgagtgcgaatgcgagtgtgaatgtgagtgtgagtgtgaatgtgaatgcgagtgtgagtgcgagtgcgaatgtgagtgtgaatgcgagtgcgaatgcgaatgcgagtgcggc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1355001_sequence/1
type: Sequence
persistent identity: BBa K1355001 sequence
display id: BBa_K1355001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaaagttaaaatgccgccagcggaactggcggctgtgggactaaggcatagctgaccttgccaggcctgcttcgccctgtagtgacgcgatcaacgggcaggaaacattcccctttcgtgcatggcaggcgcacacgagttcagacagcacggtttccatgcgcgccaagtcggccatcttctcgcgcacgtccttgagcttgtgttcggccaggctgctggcctcctcgcagtgggtgccatcgtcgagccgcaacagctcggcaatctcgtccagactgaaccccagccgctgtgccgatttcacgaatttcacccgaaccacgtccgcctccccatagcggcggatgctgccgtaaggcttgtccggttcccgcaacaggcccttgcgctgatagaagcggattgtctccacgttgaccccggccgccttggcaaaaacgccaatggtcaggttttccaaattattttccatatcgcttgactccgtacatgagtacggaagtaaggttacgctatccaatccaaattcaaaagggccaacgtatgtctgaaccacaaaacgggcgcggtgcgctcttcgccggcgggctggccgccattcttgcatcgacctgctgcctggggccgctagtactggtcgccctgggcttctccggtgcttggatcggcaacctgacggtgctggaaccctatcgaccgttgttcatcggcgcggcgctagtggcgctgttcttcgcctggaagcggatttaccggcccgtgcaggcatgcaagccaggtgaggtctgcgcgattccgcaggtgcgcgccacctacaagctgattttctggatcgtggccgtgctggtcctggtcgcgcttggatttccctatgtcgttccatttttctattaaccaggagttcatcatgaagaaactgtttgcctcccttgccctcgccgccgctgttgccccggtgtgggccgctacccagaccgtcacgctagcggttcccggcatgacttgcgccgcctgcccgatcacagtcaagaaagcgctctccaaggtcgaaggcgtgagcaaggtcgatgtgggcttcgagaagcgcgaggccgtcgtcacttttgacgacaccaaggccagcgtacagaagctgaccaaggccaccgcagacgccggctatccgtccagcgtcaagcagtga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2127007_sequence/1
type: Sequence
persistent identity: BBa K2127007 sequence
display id: BBa_K2127007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cttccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K863114_sequence/1
type: Sequence
persistent identity: BBa K863114 sequence
display id: BBa_K863114_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagcttctagagaaagacaggacccactagatggccggctcatcaatgtcagttgaattttacaactctaacaaatcagcacaaacaaactcaattacaccaataatcaaaattactaacacatctgacagtgatttaaatttaaatgacgtaaaagttagatattattacacaagtgatggtacacaaggacaaactttctggtgtgaccatgctggtgcattattaggaaatagctatgttgataacactagcaaagtgacagcaaacttcgttaaagaaacagcaagcccaacatcaacctatgatacatatgttgaatttggatttgcaggcagcaccggcatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaacatcaccatcaccatcacaccggttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K863102_sequence/1
type: Sequence
persistent identity: BBa K863102 sequence
display id: BBa_K863102_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggaaagaggagaaataatgggtccggccgggtgccaggtgctgtggggcgtcaaccagtggaacaccggcttcaccgcgaacgtcaccgtgaagaacacgtcctccgctccggtagacggctggacgctcacgttcagcttcccgtccggccagcaggtcacccaggcgtggagctcgacggtcacgcagtccggctcggccgtgacggtccgcaacgccccgtggaacggctcgatcccggcgggcggcaccgcgcagttcggcttcaacggctcgcacacgggcaccaacgccgcgccgacggcgttctcgctcaacggcacgccctgcacggtcggcggcagcaccggttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1321341_sequence/1
type: Sequence
persistent identity: BBa K1321341 sequence
display id: BBa_K1321341_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaaaccggctcatcaatgtcagttgaattttacaactctaacaaatcagcacaaacaaactcaattacaccaataatcaaaattactaacacgtctgacagtgatttaaatttaaatgacgtaaaagttagatattattacacaagtgatggtacacaaggacaaactttctggtgtgaccatgctggtgcattattaggaaatagctatgttgataacactagcaaagtgacagcaaacttcgttaaagaaacagcaagcccaacatcaacctatgatacatatgttgaatttggatttgcaggcagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1505002_sequence/1
type: Sequence
persistent identity: BBa K1505002 sequence
display id: BBa_K1505002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctactggtaccgctactgtccaagccctatgagggagcctttctagctcactgctcttcgctactatgacgaactatcgcgttgagagtagcagcggacgggcggcgcgcaagatgaggctcgcattaatgggacctgcgttcattgcggcgattggttatatcgatcccggtaactttgcgaccaatattcaggcgggtgcaagtttcggctatcagctactgtgggttgtcgtttgggccaacctgatggcgatgctgattcagatcctctctgccaaactagggattgccactggtaaaaatctggcggagcagattcgcgatcactatccgcgtcccgtagtgtggttctattgggttcaggcagaaattattgcgatggcaaccgacctggcggaatttattggtgcggcgatcggttttaaactcattcttggtgtttcgttgttgcagggcgcggtgctgacggggatcgcgactttcctgattttaatgctgcaacgtcgcgggcaaaaaccgctggagaaagtgattggcgggttactgttgtttgttgccgcggcttacattgtcgagttgattttctcccagcctaacctggcgcagctcggtaaaggaatggtgatcccgagtttacctacttcggaagcggtcttcctggcagcaggcgtgttaggggcgacgattatgccgcatgtgatttatttgcactcctcgctcactcagcatttacatggcggttcgcgtcaacaacgttattccgccaccaaatgggatgtggctatcgccatgactattgccggttttgtcaatctggcgatgatggctacagcagcggcggcgttccacttttccggtcatactggtgttgccgatcttgatgaggcttatctgacgctgcaaccgctgttaagccacgctgcggcaacggtctttggattaagcctggttgctgcggggctgtcttcaacggtggtggggacactggcggggcaggtggtgatgcagggcttcattcgctttcatatcccgctgtgggtgcgtcgtacagtcaccatgttgccgccatttattgtcattctgatgggattagatccgacacggattctggttatgagtcaggtactgttaagttttggtatcgctctggcgctggttccactgctgattttcaccagtgacagcaagttgatgggcgatctggtgaacagcaaacgcgtaaaacagacaggctgggtgattgtggtgctggtcgtggcgctgaatatctggttgttggtggggacggcgctgggattgggtggaggcggttcagtgagcaagggtgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcgcccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaacgccatcagcgacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccaagctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K863112_sequence/1
type: Sequence
persistent identity: BBa K863112 sequence
display id: BBa_K863112_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggaaagaggagaaataatgtcagttgaattttacaactctaacaaatcagcacaaacaaactcaattacaccaataatcaaaattactaacacgtctgacagtgatttaaatttaaatgacgtaaaagttagatattattacacaagtgatggtacacaaggacaaactttctggtgtgaccatgctggtgcattattaggaaatagctatgttgataacactagcaaagtgacagcaaacttcgttaaagaaacagcaagcccaacatcaacctatgatacatatgttgaatttggatttgcaggcagcaccggttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1550004_sequence/1
type: Sequence
persistent identity: BBa K1550004 sequence
display id: BBa_K1550004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaccaccgttacccagatcaaatgcgcttgcccgtcttgcctgtgcgttgtttctctgaccgaagctatcgaaaaatctggtaaatcttactgctcttctgcttgcgctgacggtcacccgaacggtaccggttgcggtcacaccggttgcgaatgccacaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K643002_sequence/1
type: Sequence
persistent identity: BBa K643002 sequence
display id: BBa_K643002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccatgggcgttatctctaaccacgcggaatcttctcgtcgtctgtaaggatcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I719005_sequence/1
type: Sequence
persistent identity: BBa I719005 sequence
display id: BBa_I719005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1321351_sequence/1
type: Sequence
persistent identity: BBa K1321351 sequence
display id: BBa_K1321351_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcgccgggttgccgtgttgactacgcggttaccaaccagtggccgggtggtttcggtgcgaacgttaccatcaccaacctgggtgacccggtttcttcttggaaactggactggacctacaccgcgggtcagcgtatccagcagctgtggaacggtaccgcgtctaccaacggtggtcaggtttctgttacctctctgccgtggaacggttctatcccgactggtggtaccgcgtctttcggtttcaacggttcttgggcgggttctaacccgaccccggcgtctttctctctgaacggtaccacctgcacgggtaccccgacgacctcgccaactcctacgccgaccccaaccacgccgactcctacaccgacgcccaccccaacgcctactccgacggtcacccccaccggcgcacaccatgaagaacaacacggcggacaccaccaccatcaccatcacaacaccaccaccactaccatggcggcgaacaccaccatcaccaccacagctctcatcatgaagaaggttgttgtagcactagcgatagtcatcatcaagaagaaggttgctgccacgggcatcacgag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1321014_sequence/1
type: Sequence
persistent identity: BBa K1321014 sequence
display id: BBa_K1321014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aatgctacgccaactaagggtgcaaccccgaccaacacagcaacgcctacaaaaagcgctacagcaacacctacaagaccgtcagttcctacaaacacaccgactaacacaccggcaaatacacctgtttcaggcaacttgaaggtcgaattctataactcaaatccgagtgatacaactaacagtattaatccgcagtttaaagtaacaaatacaggatcaagtgcaattgatctttcaaagcttacattgagatactattacaccgttgatggccagaaggaccagactttctggtgtgaccatgcagctatcataggtagcaacggctcatacaacggcatcacatcaaatgtaaaaggaacattcgtaaagatgagctcaagcacaaataacgcagacacatacctcgaaataagtttcacaggtggcactttggaacctggtgctcatgtacagatacagggtaggtttgcgaaaaatgactggagtaattatacacagtcaaatgattactcatttaagtcagcatcacagttcgtagaatgggatcaggttacagcatatttgaatggagtacttgtatggggtaaagaaccaggaggatcagtagttccgtcaacacagccggtaacaaccccaccggcaacaaccaagccgccagcaacaaccaaaccaccggctaccacgattcctccatcagacgatccg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1321112_sequence/1
type: Sequence
persistent identity: BBa K1321112 sequence
display id: BBa_K1321112_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggccggcgaatgtgaatgtgagtgcgaatgcgagtgtgaatgtgagtgtgagtgtgaatgtgaatgcgagtgtgagtgcgagtgcgaatgtgagtgtgaatgcgagtgcgaatgcgaatgcgagtgcggcaccggcggtccggccgggtgccaggtgctgtggggcgtcaaccagtggaacaccggcttcaccgcgaacgtcaccgtgaagaacacgtcctccgctccggtagacggctggacgctcacgttcagcttcccgtccggccagcaggtcacccaggcgtggagctcgacggtcacgcagtccggctcggccgtgacggtccgcaacgccccgtggaacggctcgatcccggcgggcggcaccgcgcagttcggcttcaacggctcgcacacgggcaccaacgccgcgccgacggcgttctcgctcaacggcacgccctgcacggtcggcggcagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1933100_sequence/1
type: Sequence
persistent identity: BBa K1933100 sequence
display id: BBa_K1933100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtactatgctagcactagagaaagaggagaaatactagatgaccctggacaaagctctggttctgcgtacctgcgctaacaacatggctgaccactgcggtctgatctggccggcttctggtaccgttgaatctcgttactggcagtctacccgtcgtcacgaaaacggtctggttggtctgctgtggggtgctggtacctctgctttcctgtctgttcacgctgacgctcgttggatcgtttgcgaagttgctgttgctgacatcatctctctggaagaaccgggtatggttaaattcccgcgtgctgaagttgttcacgttggtgaccgtatctctgcttctcacttcatctctgctcgtcaggctgacccggcttctacctctacctctacctctacctctaccctgaccccgatgccgaccgctatcccgaccccgatgccggctgttgcttctgttaccctgccggttgctgaacaggctcgtcacgaagttttcgacgttgcttctgtttctgctgctgctgctccggttaacaccctgccggttaccaccccgcagaacctgcaaaccgctacctacggttctaccctgtctggtgacaaccactctcgtctgatcgctggttacggttctaacgaaaccgctggtaaccactctgacctgatcggtggtcacgactgcaccctgatggctggtgaccagtctcgtctgaccgctggtaaaaactctgttctgaccgctggtgctcgttctaaactgatcggttctgaaggttctaccctgtctgctggtgaagactctaccctgatcttccgtctgtgggacggtaaacgttaccgtcagctggttgctcgtaccggtgaaaacggtgttgaagctgacatcccgtactacgttaacgaagacgacgacatcgttgacaaaccggacgaagacgacgactggatcgaagttaaaggtggcggtagtggcggtgcaggaggcggatcccatcatcatcatcatcatggtccggccgggtgccaggtgctgtggggcgtcaaccagtggaacaccggcttcaccgcgaacgtcaccgtgaagaacacgtcctccgctccggtagacggctggacgctcacgttcagcttcccgtccggccagcaggtcacccaggcgtggagctcgacggtcacgcagtccggctcggccgtgacggtccgcaacgccccgtggaacggctcgatcccggcgggcggcaccgcgcagttcggcttcaacggctcgcacacgggcaccaacgccgcgccgacggcgttctcgctcaacggcacgccctgcacggtcggcggcagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1438001_sequence/1
type: Sequence
persistent identity: BBa K1438001 sequence
display id: BBa_K1438001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaggtgatactaaagttataaattatctcaacaaactgttgggaaatgagcttgtcgcaatcaatcagtactttcttcatgcccggatgtttaaaaactggggtctcaaacgtctcaatgatgtggagtatcatgaatccattgatgagcacaaacacgccgatcgttatattgagcgcattctttttctggaaggtcttccaaacttacaggacctgggcaaactgaacattggtgaagatgttgaggaaatgctgcgttctgatctggcacttgagctggatggcgcgaagaatttgcgtgaggcaattggttatgccgatagcgttcatgattacgtcagccgcgatatgatgatagaaattttgcgcgatgaagaaggccatatcgactggctggaaacggaacttgatctgattcagaagatgggactgcaaaattatctgcaagcacagattcgcgaagaaggttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1980011_sequence/1
type: Sequence
persistent identity: BBa K1980011 sequence
display id: BBa_K1980011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttattaccctgcgcgatgatgacaacaacccgacaggttttcaataatcggacagtcggcactatcgtcgcccggacacgcgttagccagcgccagcagttggtcccgcatgctctgtaattcttcgatatggcgctcaatttccgcaactttttcaagggtacggcgtttcacatcagcagagtgacgttgcggatcgttgaacaaattgaccagttcaccgctctcctcaaggttgaaccctacttgacgggcctggcgcagtaacgtcaactcattgaggtgctgttgcgtataggtgcgataaccattctctgagcgcatcggcggagtaaccaaacctttttcttcataaaagcggattgctttgctggtcagacctgtgatcttcgccacatcggagatgttcatctgtaatttctcctctttctctagtagctagcacaatacctaggactgagctagctgtcaaactagagagtagatagatagatgcaccccgcctggtttattaatttcttgaccttccccttgctggaaggtttaacctttatcatactagaaagaggagaaatactagatgcgtgtaattcgcatgactaattatgaagccggtacacttttgacttgtagccacgaggggtgcggctgtcgtgttcgtattgaagtcccgtgtcattgtgccggagcgggcgacgcttaccgctgcacctgtggagatgaactggctcctgtgaaacatcatcaccaccatcactaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1321357_sequence/1
type: Sequence
persistent identity: BBa K1321357 sequence
display id: BBa_K1321357_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggccggccgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaaaccggcggtccggccgggtgccaggtgctgtggggcgtcaaccagtggaacaccggcttcaccgcgaacgtcaccgtgaagaacacgtcctccgctccggtagacggctggacgctcacgttcagcttcccgtccggccagcaggtcacccaggcgtggagctcgacggtcacgcagtccggctcggccgtgacggtccgcaacgccccgtggaacggctcgatcccggcgggcggcaccgcgcagttcggcttcaacggctcgcacacgggcaccaacgccgcgccgacggcgttctcgctcaacggcacgccctgcacggtcggcggcagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1355000_sequence/1
type: Sequence
persistent identity: BBa K1355000 sequence
display id: BBa_K1355000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aactttaagaaggagatatatccatgcaatcagcggatgtctcctacgccaagggcagcgccaagctcgccattgaggtcggcacgtcacccgacgcgctgacggccgctgtagctggactcggttatcgggccacgctggccgatgccccctcagtttcgacgccgggcggattgctcgacaagatgcgcgatctgctgggcagaaacgacaagacgggtagcagcggcgcattgcatatcgccgtcatcggcagcggcggggccgcgatggcagcggcgctgaaggccgtcgagcaaggcgcacgtgtcacgctgatcgagcgcggcaccatcggcggcacctgcgtcaatgtcggttgtgtgccgtccaagatcatgatccgcgccgcccatatcgcccatctgcgccgggaaagcccgttcgatggcggcatcgccgctaccacgccgaccatccagcgcacggcgctgctggcccagcagcaggcccgcgtcgatgaactgcgccacgccaagtacgaaggcatcttggagggcaatccggcgatcactgtgctgcacggctccgcccgctttaaggacaatcgcaacctgatcgtgcaactcaacgacggcggcgagcgcgtggtggcattcgaccgctgcctgatcgccaccggcgcgagcccggccgtgccgccgattcccggcctgaaagacactccgtactggacttccactgaagcgctggtcagcgagacgattcctaagcgcctggccgtgattggctcatcagtggtggcgctggagctggcgcaggcgttcgcccgactcggagcgaaggtgacgatcctggctcgcagcacgctgttcttccgcgaagacccagctataggcgaagccgtcacggccgcattccgcatggagggcatcgaggtgagggaacacacccaggccagccaggtcgcgtatatcaatggtgaaggggacggcgagttcgtgctcaccacggcgcacggcgaactgcgcgccgacaagctgctggtcgccaccggccgcgcgcccaacacacgcaagctggcactggatgcgacgggcgtcacgctcaccccgcaaggcgctatcgtcatcgaccccggcatgcgtacaagcgtggaacacatctacgccgcaggcgactgcaccgaccagccgcagttcgtctatgtggcggcagcggccggcactcgcgccgcgatcaacatgaccggcggtgacgcggccctgaacctgaccgcgatgccggccgtggtgttcaccgacccgcaagtggcgaccgtaggctacagcgaggcggaagcgcaccatgacggcatcaaaactgatagtcgcacgctaacgctggacaacgtgccgcgcgcgctcgccaacttcgacacgcgcggcttcatcaaactggtggttgaagaaggcagcggacgactgatcggcgtgcaggcagtggccccggaagcgggcgaactgatccagacggccgcactggcgattcgcaaccggatgacggtgcaggaactggccgaccagttgttcccctacctgacgatggtcgaagggttgaagctcgcggcgcagaccttcaacaaggatgtgaagcagctttcctgctgcgccgggtgaggactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1460006_sequence/1
type: Sequence
persistent identity: BBa K1460006 sequence
display id: BBa_K1460006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtactgtgctagctactagagaaagacaggaccctactagatggccaataacgcttcgttttatgcggcggcgtctatggcttacatgctaggggcaaagcatgcgtttgatgcggatcacatcgcttgcatagacaacaccattagaaagctcacccaacaaggcaaaaacgcctatggtgtggggttttacttttctatggggcattcaagcgtggtgattttaatgaccatcatcagcgcgtttgcgatcgcttgggctaaagaacacacgccgatgctagaagaaatagggggggtagtggggactttagtttctgggctttttttactcattatagggctattaaatgcgattattctcttggatttattaaaaatattcaaaaaatcgcactctaatgaaagcctgagccagcaacaaaatgaagagattgaacggcttttaacgagtaggggcttacttaatcgcttttttaaacccttgtttaattttgtctccaagtcgtggcatatttatcctatcggttttctttttgggttgggttttgataccgctagtgaaatcgcgcttttggccctctctagcagcgcgattaaagtgagtgtggtgggcatgctctctttacccattctttttgccgctggcatgagtttgtttgacactttagatggggcgttcatgctcaaggcgtatgattgggcgttcaaaacccctttaagaaaaatctattacaatatctctatcacggccttaagcgtgtttatcgcgctctttatcgggttgattgagctttttcaagtcgttagcgagaaactccatttaaaatttgaaaaccgccttctaaacaccttacaaagcctagaatttacagactttggttattacttggtgggcttatttgtaatagcgtttctaggctcattctttttatggaaaatcaagttttctaaactagagagctgaaaaaaaaaaccccgcccctgacagggcggggttttttttttactagaggtaccgagctcgtaatacgactcactatagggaatacaagctacttgttctttttgcaggtacctactagagaaagacaggaccctactagatgtcccctatactaggttattggaaaattaagggccttgtgcaacccactcgacttcttttggaatatcttgaagaaaaatatgaagagcatttgtatgagcgcgatgaaggtgataaatggcgaaacaaaaagtttgaattgggtttggagtttcccaatcttccttattatattgatggtgatgttaaattaacacagtctatggccatcatacgttatatagctgacaagcacaacatgttgggtggttgtccaaaagagcgtgcagagatttcaatgcttgaaggagcggttttggatattagatacggtgtttcgagaattgcatatagtaaagactttgaaactctcaaagttgattttcttagcaagctacctgaaatgctgaaaatgttcgaagatcgtttatgtcataaaacatatttaaatggtgatcatgtaacccatcctgacttcatgttgtatgacgctcttgatgttgttttatacatggacccaatgtgcctggatgcgttcccaaaattagtttgttttaaaaaacgtattgaagctatcccacaaattgataagtacttgaaatccagcaagtatatagcatggcctttgcagggctggcaagccacgtttggtggtggcgaccatcctccaaaatcggatctggttccgcgtggatccattcatatgatgactgtaaaaatatgtgactgtgaaggcgaatgttgtaaggactcttgtcattgtgggagcacctgccttccaagctgttctggcggtgaaaagtgcaaatgtgatcacagcaccggaagccctcaatgtaagagttgtggtgaaaaatgcaaatgcgaaaccacgtgcacttgtgaaaagag…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K519011_sequence/1
type: Sequence
persistent identity: BBa K519011 sequence
display id: BBa_K519011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgacctcaacaacgttggtcaaatgcgcttgtgagccctgtctctgcaacgtcgatcccagcaaagcgatcgatcgcaacggtctgtactactgcagcgaagcctgtgccgatggccacaccggtggtagcaaaggctgcggccacaccggctgtaactgccacggctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K863101_sequence/1
type: Sequence
persistent identity: BBa K863101 sequence
display id: BBa_K863101_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggccggcggtccggccgggtgccaggtgctgtggggcgtcaaccagtggaacaccggcttcaccgcgaacgtcaccgtgaagaacacgtcctccgctccggtagacggctggacgctcacgttcagcttcccgtccggccagcaggtcacccaggcgtggagctcgacggtcacgcagtccggctcggccgtgacggtccgcaacgccccgtggaacggctcgatcccggcgggcggcaccgcgcagttcggcttcaacggctcgcacacgggcaccaacgccgcgccgacggcgttctcgctcaacggcacgccctgcacggtcggcggcagcaccggttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1321005_sequence/1
type: Sequence
persistent identity: BBa K1321005 sequence
display id: BBa_K1321005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaatgtgaatgtgagtgcgaatgcgagtgtgaatgtgagtgtgagtgtgaatgtgaatgcgagtgtgagtgcgagtgcgaatgtgagtgtgaatgcgagtgcgaatgcgaatgcgagtgcggc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1701001_sequence/1
type: Sequence
persistent identity: BBa K1701001 sequence
display id: BBa_K1701001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaatatccagatcggcgagcttgccaagcgcaccgcatgcccggtggtgaccattcgcttctacgaacaagaagggctgttgccgccgccgggccgcagccgggggaattttcgcctgtatggcgaggagcacgtggagcgcttgcagttcattcgtcactgccggtctctggatatgccgttgagcgacgtacggaccttattgagttaccggaagcggcccgaccaggattgcggtgaagtcaatatgctcttggatgagcacatccgtcaggtcgaatctcggatcggagctttgctcgaactgaagcaccatttggtggaactgcgcgaagcctgttctggtgccaggcccgcccaatcgtgcgggattctgcagggactgtcggactgcgtgtgtgatacgcgggggaccaccgcccatccaagcgactaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1933101_sequence/1
type: Sequence
persistent identity: BBa K1933101 sequence
display id: BBa_K1933101_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtactatgctagcactagagaaagaggagaaatactagatgaccctggacaaagctctggttctgcgtacctgcgctaacaacatggctgaccactgcggtctgatctggccggcttctggtaccgttgaatctcgttactggcagtctacccgtcgtcacgaaaacggtctggttggtctgctgtggggtgctggtacctctgctttcctgtctgttcacgctgacgctcgttggatcgtttgcgaagttgctgttgctgacatcatctctctggaagaaccgggtatggttaaattcccgcgtgctgaagttgttcacgttggtgaccgtatctctgcttctcacttcatctctgctcgtcaggctgacccggcttctacctctacctctacctctacctctaccctgaccccgatgccgaccgctatcccgaccccgatgccggctgttgcttctgttaccctgccggttgctgaacaggctcgtcacgaagttttcgacgttgcttctgtttctgctgctgctgctccggttaacaccctgccggttaccaccccgcagaacctgcaaaccgctacctacggttctaccctgtctggtgacaaccactctcgtctgatcgctggttacggttctaacgaaaccgctggtaaccactctgacctgatcggtggtcacgactgcaccctgatggctggtgaccagtctcgtctgaccgctggtaaaaactctgttctgaccgctggtgctcgttctaaactgatcggttctgaaggttctaccctgtctgctggtgaagactctaccctgatcttccgtctgtgggacggtaaacgttaccgtcagctggttgctcgtaccggtgaaaacggtgttgaagctgacatcccgtactacgttaacgaagacgacgacatcgttgacaaaccggacgaagacgacgactggatcgaagttaaaggtggcggtagtggcggtgcaggaggcggatcccatcatcatcatcatcattcatcaatgtcagttgaattttacaactctaacaaatcagcacaaacaaactcaattacaccaataatcaaaattactaacacgtctgacagtgatttaaatttaaatgacgtaaaagttagatattattacacaagtgatggtacacaaggacaaactttctggtgtgaccatgctggtgcattattaggaaatagctatgttgataacactagcaaagtgacagcaaacttcgttaaagaaacagcaagcccaacatcaacctatgatacatatgttgaatttggatttgcaggcagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1471001_sequence/1
type: Sequence
persistent identity: BBa K1471001 sequence
display id: BBa_K1471001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaagttggctccttatattttagaactactgacatcagtcaatcgaactaatggtacagctgatctcttggttcctttgcttagagagctggcaaagggaagaccagtttcacgtacaactcttgctggtatattggattggccagcagagagagtcgccgctgtgcttgagcaagctacctctactgagtacgataaagacggtaatatcatcggttatggcttgacattgagagagacgtcgtacgtgttcgagatagatgatagaagactctacgcttggtgtgctttggatacgctaattttcccagcattgatcggaagaaccgcacgagtatctagtcattgtgctgctaccggtgctccagtgagcctcaccgtttcgcccagtgagattcaagctgtggagccggctgggatggcagtctccctggttctgcctcaggaggccgccgatgtccgtcaatcattctgttgccatgttcactttttcgctagtgtccctacagctgaggattgggctagtaaacaccaggggttggaagggttggctatagtgtcagtgcatgaagctttcggactaggacaggagttcaatcgtcatttattgcagacgatgagttccagaactccgtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1321094_sequence/1
type: Sequence
persistent identity: BBa K1321094 sequence
display id: BBa_K1321094_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcatcaatgtcagttgaattttacaactctaacaaatcagcacaaacaaactcaattacaccaataatcaaaattactaacacgtctgacagtgatttaaatttaaatgacgtaaaagttagatattattacacaagtgatggtacacaaggacaaactttctggtgtgaccatgctggtgcattattaggaaatagctatgttgataacactagcaaagtgacagcaaacttcgttaaagaaacagcaagcccaacatcaacctatgatacatatgttgaatttggatttgcaggcagcaccggcgaatgtgaatgtgagtgcgaatgcgagtgtgaatgtgagtgtgagtgtgaatgtgaatgcgagtgtgagtgcgagtgcgaatgtgagtgtgaatgcgagtgcgaatgcgaatgcgagtgcggc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K863111_sequence/1
type: Sequence
persistent identity: BBa K863111 sequence
display id: BBa_K863111_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggccggctcatcaatgtcagttgaattttacaactctaacaaatcagcacaaacaaactcaattacaccaataatcaaaattactaacacgtctgacagtgatttaaatttaaatgacgtaaaagttagatattattacacaagtgatggtacacaaggacaaactttctggtgtgaccatgctggtgcattattaggaaatagctatgttgataacactagcaaagtgacagcaaacttcgttaaagaaacagcaagcccaacatcaacctatgatacatatgttgaatttggatttgcaggcagcaccggttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1321002_sequence/1
type: Sequence
persistent identity: BBa K1321002 sequence
display id: BBa_K1321002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcatcaatgtcagttgaattttacaactctaacaaatcagcacaaacaaactcaattacaccaataatcaaaattactaacacgtctgacagtgatttaaatttaaatgacgtaaaagttagatattattacacaagtgatggtacacaaggacaaactttctggtgtgaccatgctggtgcattattaggaaatagctatgttgataacactagcaaagtgacagcaaacttcgttaaagaaacagcaagcccaacatcaacctatgatacatatgttgaatttggatttgcaggcagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1393000_sequence/1
type: Sequence
persistent identity: BBa K1393000 sequence
display id: BBa_K1393000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaactgaagaacaccttaggcgttgtcatcggctcgctggttgccgcttcggcaatgaacgcctttgcccagggccagaactcggtagagatcgaagccttcggcaagcgctacttcaccgacagcgttcgcaacatgaagaacgcggacctgtacggcggctcgatcggttacttcctgaccgacgacgtcgagctggcgctgtcctacggtgagtaccatgacgttcgtggcacctacgaaaccggcaacaagaaggtccacggcaacctgacctccctggacgccatctaccacttcggtaccccgggcgtaggtctgcgtccgtacgtgtcggctggtctggctcaccagaacatcaccaacatcaacagcgacagccaaggccgtcagcagatgaccatggccaacatcggcgctggtctgaagtactacttcaccgagaacttcttcgccaaggccagcctcgacggccagtacggtctggagaagcgtgacaacggtcaccagggcgagtggatggctggcctgggcgtcggcttcaacttcggtggttcgaaaggtggcggaggttcatccccgcatcatggcggctgg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2127006_sequence/1
type: Sequence
persistent identity: BBa K2127006 sequence
display id: BBa_K2127006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cctgtagagaagagtccctgaatatcaaaatggtgggataaaaagctcaaaaaggaaagtaggctgtggttccctaggcaacagtcttccctaccccactggaaactaaaaaaacgagaaaagttcgcaccgaacatcaattgcataattttagccctaaaacataagctgaacgaaactggttg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1321105_sequence/1
type: Sequence
persistent identity: BBa K1321105 sequence
display id: BBa_K1321105_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggagatactagagaaagaggagaaatactagatggccggcggtccggccgggtgccaggtgctgtggggcgtcaaccagtggaacaccggcttcaccgcgaacgtcaccgtgaagaacacgtcctccgctccggtagacggctggacgctcacgttcagcttcccgtccggccagcaggtcacccaggcgtggagctcgacggtcacgcagtccggctcggccgtgacggtccgcaacgccccgtggaacggctcgatcccggcgggcggcaccgcgcagttcggcttcaacggctcgcacacgggcaccaacgccgcgccgacggcgttctcgctcaacggcacgccctgcacggtcggcggcagcaccggcgaatgtgaatgtgagtgcgaatgcgagtgtgaatgtgagtgtgagtgtgaatgtgaatgcgagtgtgagtgcgagtgcgaatgtgagtgtgaatgcgagtgcgaatgcgaatgcgagtgcggc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1321111_sequence/1
type: Sequence
persistent identity: BBa K1321111 sequence
display id: BBa_K1321111_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggccggcgaatgtgaatgtgagtgcgaatgcgagtgtgaatgtgagtgtgagtgtgaatgtgaatgcgagtgtgagtgcgagtgcgaatgtgagtgtgaatgcgagtgcgaatgcgaatgcgagtgcggcaccggctcatcaatgtcagttgaattttacaactctaacaaatcagcacaaacaaactcaattacaccaataatcaaaattactaacacgtctgacagtgatttaaatttaaatgacgtaaaagttagatattattacacaagtgatggtacacaaggacaaactttctggtgtgaccatgctggtgcattattaggaaatagctatgttgataacactagcaaagtgacagcaaacttcgttaaagaaacagcaagcccaacatcaacctatgatacatatgttgaatttggatttgcaggcagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K643000_sequence/1
type: Sequence
persistent identity: BBa K643000 sequence
display id: BBa_K643000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gccatcatcatcatcatcacggcgatgtgcatcatcatggccgccacggcgcggaacatgcggatatttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K863113_sequence/1
type: Sequence
persistent identity: BBa K863113 sequence
display id: BBa_K863113_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggaaagaggagaaataatgtcagttgaattttacaactctaacaaatcagcacaaacaaactcaattacaccaataatcaaaattactaacacgtctgacagtgatttaaatttaaatgacgtaaaagttagatattattacacaagtgatggtacacaaggacaaactttctggtgtgaccatgctggtgcattattaggaaatagctatgttgataacactagcaaagtgacagcaaacttcgttaaagaaacagcaagcccaacatcaacctatgatacatatgttgaatttggatttgcaggcagcaccggcatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaacatcaccatcaccatcacaccggttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1980001_sequence/1
type: Sequence
persistent identity: BBa K1980001 sequence
display id: BBa_K1980001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcaacgtcgtgacttcctgaaatacagtgttgctcttggggttgcttccgcattacccctttggtcccgcgcagtgtttgctggtgaagacccgcacgcaggccataagatgtctcatggtgcgaagtacaaagcacttcttgactcttcctcccattgtgttgcggtaggcgaggactgtctgcgtcactgttttgaaatgcttgctatgaacgacgcgagtatgggggcgtgcaccaaagccacctacgatcttgtagccgcctgcggagcattagctaagctggcggggaccaactcagcctttacaccagccttcgccaaagtcgttgctgatgtatgtgccgcgtgcaaaaaggaatgtgacaagtttccatccattgccgaatgtaaggcttgcggagaggcgtgccaggcttgtgcagaagagtgtcacaaagtcgcggccggcagtgggtctgggtcgagcaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtccgtggagagggtgaaggtgatgctacaaacggaaaactcacccttaaatttatttgcactactggaaaactacctgttccctggccaacacttgtcactactctgacctatggtgttcaatgcttttcccgttatccggatcacatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggacctacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaaaggtattgattttaaagaagatggaaacattctcggacacaaactcgagtacaactttaactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacgttgaagatggttccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgtcctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaacatcatcaccaccatcactaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2127001_sequence/1
type: Sequence
persistent identity: BBa K2127001 sequence
display id: BBa_K2127001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cctgtagagaagagtccctgaatatcaaaatggtgggataaaaagctcaaaaaggaaagtaggctgtggttccctaggcaacagtcttccctaccccactggaaactaaaaaaacgagaaaagttcgcaccgaacatcaattgcataattttagccctaaaacataagctgaacgaaactggttgcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaattaaagaggagaaaatgggactaccttggtatcgcgttcatacagttgtcctgaatgatccagggcgactcatctctgtccatttaatgcacactgccctagtggctggctgggctggatctatggctctctatgagttggccatttttgactccagcgatgctgtgcttaaccccatgtggcggcaaggcatgtttgttttgcccttcatggcccgcctcggtgtcaccagttcctggaatggctggagcgtcaccggagaaactggtttggatcccggtttctggtcctttgaaggggtagctgctgcccacatcgttctatctggtctgttgttcctagccgccgtatggcactgggtattttgggacctggaattatttgttgacccccgtaccggtgaatccgcattggatttgcccaaaatgtttggtattcacctcttcttgtctggtttgctctgcttcggttttggtgctttccacctcaccggcgtttggggccctggcatgtgggtatccgacccctatggtctgacgggccacgtacagccagtggcaccggaatggggaccagctggctttaacccgtttaaccccggtggggtagtggctcaccacattgctgctggtattgtcggtatcattgccggtcttttccaccttacggtacggccccctgagcgcttgtataaagccctccgcatggggaatattgagaccgtattgtccagtagtattgccgctgtattttttgccgctttcgtcgtggccggtaccatgtggtacggtaatgccaccacccccatcgaactctttggccccacccgttatcagtgggataagggttattttcaagaagaaatccaacgccgggttgatagccagttagcagaaggagcttccctatctgaagcctggtccaccatccctgaaaagttggctttctacgattacgtcggtaacagccccgctaaaggtggtttgtttcgtaccggtgctatgaacagtggtgatggcattgcccaggaatggattggtcaccccatattcaaagacaaagaaggtcgggaactggaggtacggcgtatgcctaacttctttgaaactttccccgtcatcatgaccgatgcggatggtgtagtccgggcggatattcccttccgtcgttccgagtctaaattcagtgtggaacaaaccggtgttaccgtcagcttctacggtggtgctttagacggccagaccttcagcaatcccagtgatgtgaagaagtttgcccggaaagctcagttgggtgaaggcttcgacttcgatacggaaaccttcaactctgatggtgtattccgcaccagtccccggggttggtttacctttggccacgctgtctttgccttgctcttcttctttggtcacatctggcatggttcccggactctgttccgtgacgtatttgctggggttgatcctggtttggaagaacaagtggaatttggtgtgtttgctaaggttggtgacctttccacccggaaagaagcccaccatcaccatcaccatgtgtagtagcttccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1393001_sequence/1
type: Sequence
persistent identity: BBa K1393001 sequence
display id: BBa_K1393001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaactgaagaacaccttaggcgttgtcatcggctcgctggttgccgcttcggcaatgaacgcctttgcccagggccagaactcggtagagatcgaagccttcggcaagcgctacttcaccgacagcgttcgcaacatgaagaacgcggacctgtacggcggctcgatcggttacttcctgaccgacgacgtcgagctggcgctgtcctacggtgagtaccatgacgttcgtggcacctacgaaaccggcaacaagaaggtccacggcaacctgacctccctggacgccatctaccacttcggtaccccgggcgtaggtctgcgtccgtacgtgtcggctggtctggctcaccagaacatcaccaacatcaacagcgacagccaaggccgtcagcagatgaccatggccaacatcggcgctggtctgaagtactacttcaccgagaacttcttcgccaaggccagcctcgacggccagtacggtctggagaagcgtgacaacggtcaccagggcgagtggatggctggcctgggcgtcggcttcaacttcggtggttcgaaagccgctccggctccggaaccggtttccccgcatcatggcggctgg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1438003_sequence/1
type: Sequence
persistent identity: BBa K1438003 sequence
display id: BBa_K1438003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaacgatcaacgctgcgcgtgtacgcactgttcctgcactgtggatgccaatgccttgcagcgcgacggcaaggcctattgctgcgaggcctgcgccagcggccaccgcaagggtgagccctgccggatgcaggactgccattgtggtgagaagccgggcgagagcgcggtggacaatgcgttggatgaaaccttcccagcgagtgatccgatctcgcccggtggttctggtggtggttctggtatggctatggcgagtttatatcggcgatctcttccttctcctccggccattgacttttcttccgccgaaggcaagctaatcttcaatgaagcgcttcaaaaaggaactatggaaggatttttcaggttgatttcgtattttcagacacaatccgaacctgcgtattgtggtttggctagtctctcagtggtgttgaatgctctttctatcgatcctggacgtaaatggaaagggccttggaggtggtttgatgaatcaatgttggattgctgcgaacctctggaagtagtgaaggaaaaaggcatttcatttggaaaagttgtctgtttggctcattgttcaggagcaaaagttgaggctttccgtacaagtcagagcaccattgatgatttccgcaaatttgtcgtcaaatgcacgagttctgagaattgtcatatgatctcaacatatcaccgaagtgtatttaagcagactgggaatggtcacttttcacctattggtggctataatgctgagagagatatggctttgattcttgatgttgctcgtttcaagtatccccctcactgggttcctcttaaacttctttgggaagccatggacagtattgatcagtcaacagggaaacgtagagggttcatgctcatatctcgaccacacagagaacccggattgctctatactctgagctgcaaggatgaaagctggatcgaaatagccaagtatttgaaggaagatgttcctcgtcttgtaagttcacagcatgtagattctgtggagaaaatcatatcagttgtgttcaagtcacttccatcaaatttcaaccaattcatcagatgggtggctgagatccgaattacagaggactcaaaccaaaatctcagcgcagaggagaagtctaggctgaaactaaagcaattggtgctgaaggaagtgcacgaaactgaactgttcaaacacatcaataagttcttatccacagtgggttatgaagacagtctgacttatgctgctgcaaaggcttgttgccaaggagctgaaatcttatccggaagcccatcaaaagagttttgttgtcgggaaacttgcgtgaaatgcatcaaaggtcctgatgactctgaaggcacggtggtgactggagttgtggtgcgtgatgggaatgaacaaaaggttgatctgttagtgccatcgacgcaaactgagtgtgaatgtggtcctgaagcaacttatccagcaggaaacgatgtgttcactgcacttctattggctttacctccacagacatggtcagggatcaaagaccaagctcttatgcatgaaatgaagcagctcatttccatggcttccctcccaactttgcttcaagaagaggtattgcatcttcgacggcaacttcagctgctaaaacgatgccaagagaacaaggaagaggatgatctcgctgctcctgcctattaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1420001_sequence/1
type: Sequence
persistent identity: BBa K1420001 sequence
display id: BBa_K1420001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgacccatctaaaaatcaccggcatgacctgcgactcgtgcgcggcgcacgtcaaggaagcgctggaaaaagtacccggcgtccaatctgccatagtgtcctatgccaagggcgcggcccagctcgcccttgatccaggcacagcgccggacgcactgaccgccgccgtggctggcctgggctacaaagcgatgctcgccgatgccccgccgaccgacaaccgcactgggctgttcgacaaggtgcgcggctggatgggtgccgccgacaagggcagcggcggcgagcgcccgttgcaagtcgccgtgatcggcagcggtggagccgcgatggcggcagcactgaaggccgtcgagcaaggcgcgcaggtcacgctgattgagcgcggcaccatcggcggcacctgcgtcaacgtcggttgtgtgccgtccaagatcatgatccgcgccgcccacatcgcccatctgcgccgggaaagcccattcgacggcggcatgccacccacaccgccgacgatcttgcgcgagcggctgctggcccagcagcaggcccgtgtcgaagaactccgtcatgccaagtacgaaggcatcctggacggcaattcagccatcaccgttctgcacggtgaagcgcgtttcaaggacgaccagagccttatcgttagtttgaacgagggtggtgagcgcgtcgtgatgttcgaccgctgcctggtcgccacgggtgccagtccggccatgccgccgattccgggcctgaaagagtcaccctactggacttcgaccgaggccttggtcagcgacaccattcccgaacgcctagccgtgatcggctcgtcggtggtcgcgctggaactggcgcaagccttcgcccggctgggcagccaggtcacgatcctagctcgcaacacgctgttcttccgcgacgacccgtccatcggcgaggccgtcacagcggccttccgtgccgaagggatcaaggtactggaacacacgcaagccagccaggtcgcgcatgtgaacggcgagttcgtgctgaccacgggacacggtgaagtacgtgccgacaagctgctggtcgctaccggacggacaccgaacacgcgcagcctggcactcgacgcggcgggagtcaccgtcaatgcgcagggtgccatcgtcatcgacaagggcatgcgcaccagcacgccacacatttacgcggccggcgactgcacggaccagccacaattcgtctatgtggcggcagcggccggcacccgtgccgcgatcaacatgaccggaggggatgctgccatcaatctaaccgcgatgccggccgtggtgttcaccgatccgcaagtagcaaccgtgggctacagcgaagcggaagcgcatcacgacgggatcgaaaccgacagtcgcacgctcacgctcgacaacgtgccgcgtgcgcttgccaacttcgacacacgcggctttatcaagctggtcatcgaggaaggtagcggacggctcatcggcgtgcaggtcgtcgccccggaagccggcgaattgatccagacggctgttctcgccattcgcaaccgcatgacggtgcaggaactggccgaccagttgttcccctacctgacgatggtcgaggggctgaagctcgcggcgcagaccttcaccaaggatgtcaaacaattgtcctgctgcgcaggatga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2127004_sequence/1
type: Sequence
persistent identity: BBa K2127004 sequence
display id: BBa_K2127004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgggactaccttggtatcgcgttcatacagttgtcctgaatgatccagggcgactcatctctgtccatttaatgcacactgccctagtggctggctgggctggatctatggctctctatgagttggccatttttgactccagcgatgctgtgcttaaccccatgtggcggcaaggcatgtttgttttgcccttcatggcccgcctcggtgtcaccagttcctggaatggctggagcgtcaccggagaaactggtttggatcccggtttctggtcctttgaaggggtagctgctgcccacatcgttctatctggtctgttgttcctagccgccgtatggcactgggtattttgggacctggaattatttgttgacccccgtaccggtgaatccgcattggatttgcccaaaatgtttggtattcacctcttcttgtctggtttgctctgcttcggttttggtgctttccacctcaccggcgtttggggccctggcatgtgggtatccgacccctatggtctgacgggccacgtacagccagtggcaccggaatggggaccagctggctttaacccgtttaaccccggtggggtagtggctcaccacattgctgctggtattgtcggtatcattgccggtcttttccaccttacggtacggccccctgagcgcttgtataaagccctccgcatggggaatattgagaccgtattgtccagtagtattgccgctgtattttttgccgctttcgtcgtggccggtaccatgtggtacggtaatgccaccacccccatcgaactctttggccccacccgttatcagtgggataagggttattttcaagaagaaatccaacgccgggttgatagccagttagcagaaggagcttccctatctgaagcctggtccaccatccctgaaaagttggctttctacgattacgtcggtaacagccccgctaaaggtggtttgtttcgtaccggtgctatgaacagtggtgatggcattgcccaggaatggattggtcaccccatattcaaagacaaagaaggtcgggaactggaggtacggcgtatgcctaacttctttgaaactttccccgtcatcatgaccgatgcggatggtgtagtccgggcggatattcccttccgtcgttccgagtctaaattcagtgtggaacaaaccggtgttaccgtcagcttctacggtggtgctttagacggccagaccttcagcaatcccagtgatgtgaagaagtttgcccggaaagctcagttgggtgaaggcttcgacttcgatacggaaaccttcaactctgatggtgtattccgcaccagtccccggggttggtttacctttggccacgctgtctttgccttgctcttcttctttggtcacatctggcatggttcccggactctgttccgtgacgtatttgctggggttgatcctggtttggaagaacaagtggaatttggtgtgtttgctaaggttggtgacctttccacccggaaagaagcccaccatcaccatcaccatgtgtagtag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1933103_sequence/1
type: Sequence
persistent identity: BBa K1933103 sequence
display id: BBa_K1933103_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtactatgctagcactagagaaagaggagaaatactagatggcgttcgacccgaacctggttggtccgaccctgccgccgatcccgccgttcaccctgccgacccatcatcatcatcatcattcatcaatgtcagttgaattttacaactctaacaaatcagcacaaacaaactcaattacaccaataatcaaaattactaacacgtctgacagtgatttaaatttaaatgacgtaaaagttagatattattacacaagtgatggtacacaaggacaaactttctggtgtgaccatgctggtgcattattaggaaatagctatgttgataacactagcaaagtgacagcaaacttcgttaaagaaacagcaagcccaacatcaacctatgatacatatgttgaatttggatttgcaggcagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1321095_sequence/1
type: Sequence
persistent identity: BBa K1321095 sequence
display id: BBa_K1321095_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggtccggccgggtgccaggtgctgtggggcgtcaaccagtggaacaccggcttcaccgcgaacgtcaccgtgaagaacacgtcctccgctccggtagacggctggacgctcacgttcagcttcccgtccggccagcaggtcacccaggcgtggagctcgacggtcacgcagtccggctcggccgtgacggtccgcaacgccccgtggaacggctcgatcccggcgggcggcaccgcgcagttcggcttcaacggctcgcacacgggcaccaacgccgcgccgacggcgttctcgctcaacggcacgccctgcacggtcggcggcagctactagagaccggctactagaggaatgtgaatgtgagtgcgaatgcgagtgtgaatgtgagtgtgagtgtgaatgtgaatgcgagtgtgagtgcgagtgcgaatgtgagtgtgaatgcgagtgcgaatgcgaatgcgagtgcggc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1933000_sequence/1
type: Sequence
persistent identity: BBa K1933000 sequence
display id: BBa_K1933000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcgttcgacccgaacctggttggtccgaccctgccgccgatcccgccgttcaccctgccgacccatcatcatcatcatcat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K190020_sequence/1
type: Sequence
persistent identity: BBa K190020 sequence
display id: BBa_K190020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgagggtgatacgaatgacgaactacgaggctgggaccttgctgacctgtagccacgagggctgtggctgtcgggttcgcatcgaagtcccatgccactgtgctggcgccggtgacgcctaccgctgcacctgcggcgacgaattggccccggtcaagtag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1321345_sequence/1
type: Sequence
persistent identity: BBa K1321345 sequence
display id: BBa_K1321345_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcacaccatgaagaacaacacggcggacaccaccaccatcaccatcacaacaccaccaccactaccatggcggcgaacaccaccatcaccaccacagctctcatcatgaagaaggttgttgtagcactagcgatagtcatcatcaagaagaaggttgctgccacgggcatcacgagaccggccccggcgccaatccgcccggtactacgaccacttcgcgtccagcaaccacgaccgggtcgagccccggcccacaggcctgttcttccgtctggggtcagtgcggcggacagaactggtctggccctacctgctgtgcttctggcagcacatgcgtctactctaacgactactatagccagtgcctgccgggtgctaacccgccaggcactaccactacctctcgccccgctaccactacaggctctagcccgggacctacccaaagccactacggccagtgcggaggcattggctacagcggccctaccgtctgcgcctccggaaccacctgccaagtcctgaacccgtactactcccagtgtctt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1509000_sequence/1
type: Sequence
persistent identity: BBa K1509000 sequence
display id: BBa_K1509000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctagcgacactcttgtaagtgatcgagggcgttttgataaagcgccacaatgtgatgatcctgtagctggtagtagacatgccgcccttgcttgcgatagctcaccagccgcagattacggagcgatcgcaattggtgagacaccgccgattcggaaacaccaattgcctgggccaaatccccaacacagagctccgatcgcgctaacagggacagcaaccgcagtcgatttggatcggccagcactgcaaaaaattcggctagcgattgggcaacttcgggtgcgatcgcttgaagctccgaggcgatcgccgcatgagtcccttggcagactaccgtctctccgtcctgcagcactggttttgtcat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1460002_sequence/1
type: Sequence
persistent identity: BBa K1460002 sequence
display id: BBa_K1460002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggtacctactagagaaagacaggaccctactagatgtcccctatactaggttattggaaaattaagggccttgtgcaacccactcgacttcttttggaatatcttgaagaaaaatatgaagagcatttgtatgagcgcgatgaaggtgataaatggcgaaacaaaaagtttgaattgggtttggagtttcccaatcttccttattatattgatggtgatgttaaattaacacagtctatggccatcatacgttatatagctgacaagcacaacatgttgggtggttgtccaaaagagcgtgcagagatttcaatgcttgaaggagcggttttggatattagatacggtgtttcgagaattgcatatagtaaagactttgaaactctcaaagttgattttcttagcaagctacctgaaatgctgaaaatgttcgaagatcgtttatgtcataaaacatatttaaatggtgatcatgtaacccatcctgacttcatgttgtatgacgctcttgatgttgttttatacatggacccaatgtgcctggatgcgttcccaaaattagtttgttttaaaaaacgtattgaagctatcccacaaattgataagtacttgaaatccagcaagtatatagcatggcctttgcagggctggcaagccacgtttggtggtggcgaccatcctccaaaatcggatctggttccgcgtggatccattcatatgatgactgtaaaaatatgtgactgtgaaggcgaatgttgtaaggactcttgtcattgtgggagcacctgccttccaagctgttctggcggtgaaaagtgcaaatgtgatcacagcaccggaagccctcaatgtaagagttgtggtgaaaaatgcaaatgcgaaaccacgtgcacttgtgaaaagagtaaatgcaattgtgaaaaatgttagaaaaaaaaaccccgcccctgacagggcggggttttttttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1321104_sequence/1
type: Sequence
persistent identity: BBa K1321104 sequence
display id: BBa_K1321104_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggagatactagagaaagaggagaaatactagatggccggctcatcaatgtcagttgaattttacaactctaacaaatcagcacaaacaaactcaattacaccaataatcaaaattactaacacgtctgacagtgatttaaatttaaatgacgtaaaagttagatattattacacaagtgatggtacacaaggacaaactttctggtgtgaccatgctggtgcattattaggaaatagctatgttgataacactagcaaagtgacagcaaacttcgttaaagaaacagcaagcccaacatcaacctatgatacatatgttgaatttggatttgcaggcagcaccggcgaatgtgaatgtgagtgcgaatgcgagtgtgaatgtgagtgtgagtgtgaatgtgaatgcgagtgtgagtgcgagtgcgaatgtgagtgtgaatgcgagtgcgaatgcgaatgcgagtgcggc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1321152_sequence/1
type: Sequence
persistent identity: BBa K1321152 sequence
display id: BBa_K1321152_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcaggtacaggttgtaaaatttgggaagattgtaaatgtggtgcagcatgtagctgtggtgatagctgtacctgtggcaccgttaaaaaaggtacaaccagccgtgccggtgcaggttgtccgtgtggtccgaaatgtaaatgtacaggtcagggtagctgtaactgcgttaaagatgattgttgtggttgtggcaaaaccggcggtccggccgggtgccaggtgctgtggggcgtcaaccagtggaacaccggcttcaccgcgaacgtcaccgtgaagaacacgtcctccgctccggtagacggctggacgctcacgttcagcttcccgtccggccagcaggtcacccaggcgtggagctcgacggtcacgcagtccggctcggccgtgacggtccgcaacgccccgtggaacggctcgatcccggcgggcggcaccgcgcagttcggcttcaacggctcgcacacgggcaccaacgccgcgccgacggcgttctcgctcaacggcacgccctgcacggtcggcggcagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1321346_sequence/1
type: Sequence
persistent identity: BBa K1321346 sequence
display id: BBa_K1321346_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcatcaatgtcagttgaattttacaactctaacaaatcagcacaaacaaactcaattacaccaataatcaaaattactaacacgtctgacagtgatttaaatttaaatgacgtaaaagttagatattattacacaagtgatggtacacaaggacaaactttctggtgtgaccatgctggtgcattattaggaaatagctatgttgataacactagcaaagtgacagcaaacttcgttaaagaaacagcaagcccaacatcaacctatgatacatatgttgaatttggatttgcaggcagcaccggccgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z