##gff-version 3
##sequence-region BBa_Z0152 3 690
BBa_Z0152	.	point_mutation	690	690	.	+	0	ID=annotation1849919;Name=a->G mutation to remove RBS
BBa_Z0152	.	promoter	16	20	.	+	0	ID=annotation1860893;Name=S2 -35 region
BBa_Z0152	.	promoter	40	45	.	+	0	ID=annotation1860894;Name=S2 -10 region
BBa_Z0152	.	point_mutation	16	18	.	+	0	ID=annotation1860895;Name=ttc -> TTT
BBa_Z0152	.	promoter	3	66	.	+	0	ID=annotation1849934;Name=S2: possible promoter
BBa_Z0152	.	point_mutation	4	39	.	+	0	ID=annotation1881251;Name=ggc...gct->ggc..gct codon shuffle
>BBa_Z0152
atggcgaaaaaaatatttacaagtgcgttaggaacagctgaaccttacgcttacatcgccaagccggactacggcaacg
aagagcgtggctttgggaaccctcgtggtgtctataaagttgacctgactattcccaacaaagacccgcgctgccagcg
tatggtcgatgaaatcgtgaagtgtcacgaagaggcttatgctgctgccgttgaggaatacgaagctaatccacctgct
gtagctcgtggtaagaaaccgctgaaaccgtatgagggtgacatgccgttcttcgataacggtgacggtacgactacct
ttaagttcaaatgctacgcgtctttccaagacaagaagaccaaagagaccaagcacatcaatctggttgtggttgactc
aaaaggtaagaagatggaagacgttccgattatcggtggtggctctaagctgaaagttaaatattctctggttccatac
aagtggaacactgctgtaggtgcgagcgttaagctgcaactggaatccgtgatgctggtcgaactggctacctttggtg
gcggtgaagacgattgggctgacgaagttgaagagaacggctatgttgcctctggttctgccaaagcgagcaaaccacg
cgacgaagaaagctgggacgaagacgacgaagagtccgaggaagcagacgaagacggggacttctaa