##gff-version 3
##sequence-region BBa_T9003 1 1603
BBa_T9003	.	engineered_region	1070	1603	.	+	0	ID=BBa_E0435;Name=BBa_E0435
BBa_T9003	.	terminator	1475	1603	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_E0435
BBa_T9003	.	terminator	1475	1554	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_T9003	.	engineered_region	1475	1554	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_T9003	.	stem_loop	1486	1529	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_T9003	.	terminator	1563	1603	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_T9003	.	engineered_region	1563	1603	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_T9003	.	stop_codon	1596	1596	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_T9003	.	stem_loop	1570	1589	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_T9003	.	polyA_site	1590	1603	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_T9003	.	ribosome_entry_site	1070	1081	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0435
BBa_T9003	.	conserved_region	1074	1077	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_T9003	.	engineered_region	1088	1466	.	+	0	ID=BBa_E0035;Name=BBa_E0035;Parent=BBa_E0435
BBa_T9003	.	sequence_alteration	1435	1435	.	+	0	ID=annotation1959045;Name=inserted T;Parent=BBa_E0035
BBa_T9003	.	sequence_alteration	1168	1168	.	+	0	ID=annotation1959040;Name=T;Parent=BBa_E0035
BBa_T9003	.	promoter	1113	1122	.	+	0	ID=annotation1959037;Name=T3 promoter;Parent=BBa_E0035
BBa_T9003	.	promoter	1260	1278	.	+	0	ID=annotation1959038;Name=T7 promoter;Parent=BBa_E0035
BBa_T9003	.	CDS	1088	1441	.	+	0	ID=annotation1959036;Name=lacZ alpha;Parent=BBa_E0035
BBa_T9003	.	sequence_feature	1088	1102	.	+	0	ID=annotation1959035;Name=lacZ gene fragment;Parent=BBa_E0035
BBa_T9003	.	sequence_alteration	1195	1195	.	+	0	ID=annotation1959042;Name=C;Parent=BBa_E0035
BBa_T9003	.	sequence_alteration	1198	1198	.	+	0	ID=annotation1959043;Name=G;Parent=BBa_E0035
BBa_T9003	.	sequence_alteration	1165	1165	.	+	0	ID=annotation1959039;Name=C;Parent=BBa_E0035
BBa_T9003	.	engineered_tag	1442	1466	.	+	0	ID=annotation1959046;Name=barcode;Parent=BBa_E0035
BBa_T9003	.	sequence_feature	1286	1435	.	+	0	ID=annotation1959044;Name=lacZ gene fragment;Parent=BBa_E0035
BBa_T9003	.	sequence_alteration	1174	1174	.	+	0	ID=annotation1959041;Name=A;Parent=BBa_E0035
BBa_T9003	.	engineered_region	1	1061	.	+	0	ID=BBa_F2620;Name=BBa_F2620
BBa_T9003	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_F2620
BBa_T9003	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_T9003	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_F2620
BBa_T9003	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_T9003	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_T9003	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_T9003	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_T9003	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_T9003	.	terminator	870	998	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_F2620
BBa_T9003	.	terminator	870	949	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_T9003	.	engineered_region	870	949	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_T9003	.	stem_loop	881	924	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_T9003	.	terminator	958	998	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_T9003	.	engineered_region	958	998	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_T9003	.	stop_codon	991	991	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_T9003	.	stem_loop	965	984	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_T9003	.	polyA_site	985	998	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_T9003	.	CDS	81	836	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_F2620
BBa_T9003	.	point_mutation	572	572	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_T9003	.	CDS	81	830	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_T9003	.	engineered_region	81	836	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_T9003	.	engineered_tag	837	861	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_T9003	.	point_mutation	254	254	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_T9003	.	engineered_region	81	82	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_T9003	.	promoter	1007	1061	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_F2620
BBa_T9003	.	promoter	1048	1053	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_T9003	.	engineered_region	1007	1061	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_T9003	.	promoter	1026	1031	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_T9003	.	start_codon	1059	1059	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_T9003	.	non_covalent_binding_site	1007	1026	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
>BBa_T9003
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaat
caatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggtta
aatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatga
tcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaa
tctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatg
gcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacat
accattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaacc
aaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtg
agcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagc
aattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagag
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtt
tacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatgaccatgattacgccaa
gcgcgcaattaaccctcactaaagggaacaaaagctggagctccaccgcggtggcggcagcactagagctagtggatcc
cccgggctgtagaaattcgatatcaagcttatcgataccgtcgacctcgagggggggcccggtacccaattcgccctat
agtgagtcgtattacgcgcgctcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaactta
atcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagtt
gcgcagcctgaattaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctc
agtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttc
gggtgggcctttctgcgtttata