##gff-version 3
##sequence-region BBa_T5000 1 4456
BBa_T5000	.	terminator	2398	2526	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_T5000	.	terminator	2398	2477	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_T5000	.	engineered_region	2398	2477	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_T5000	.	stem_loop	2409	2452	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_T5000	.	terminator	2486	2526	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_T5000	.	stop_codon	2519	2519	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_T5000	.	polyA_site	2513	2526	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_T5000	.	stem_loop	2493	2512	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_T5000	.	engineered_region	2486	2526	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_T5000	.	CDS	2596	3351	.	+	0	ID=BBa_C0362;Name=BBa_C0362
BBa_T5000	.	ribosome_entry_site	3579	3590	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_T5000	.	conserved_region	3583	3586	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_T5000	.	promoter	2535	2569	.	+	0	ID=BBa_J23101;Name=BBa_J23101
BBa_T5000	.	promoter	1	1210	.	+	0	ID=BBa_I0500;Name=BBa_I0500
BBa_T5000	.	promoter	1043	1114	.	+	0	ID=annotation1498842;Name=PC;Parent=BBa_I0500
BBa_T5000	.	sequence_feature	1	1079	.	+	0	ID=annotation1498843;Name=AraC;Parent=BBa_I0500
BBa_T5000	.	promoter	1080	1210	.	+	0	ID=annotation1498841;Name=Pbad;Parent=BBa_I0500
BBa_T5000	.	CDS	3597	4319	.	+	0	ID=BBa_E0030;Name=BBa_E0030
BBa_T5000	.	terminator	3360	3488	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_T5000	.	terminator	3360	3439	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_T5000	.	engineered_region	3360	3439	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_T5000	.	stem_loop	3371	3414	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_T5000	.	terminator	3448	3488	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_T5000	.	stop_codon	3481	3481	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_T5000	.	polyA_site	3475	3488	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_T5000	.	stem_loop	3455	3474	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_T5000	.	engineered_region	3448	3488	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_T5000	.	CDS	1237	2364	.	+	0	ID=BBa_C0012;Name=BBa_C0012
BBa_T5000	.	engineered_tag	2365	2389	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_T5000	.	engineered_region	1237	2364	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_T5000	.	tag	2326	2364	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_T5000	.	CDS	1237	2364	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_T5000	.	ribosome_entry_site	2578	2589	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_T5000	.	conserved_region	2582	2585	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_T5000	.	promoter	3497	3570	.	+	0	ID=BBa_I751502;Name=BBa_I751502
BBa_T5000	.	sequence_feature	3538	3544	.	+	0	ID=annotation1955561;Name=-10;Parent=BBa_I751502
BBa_T5000	.	non_covalent_binding_site	3497	3515	.	+	0	ID=annotation1955564;Name=luxR binding site;Parent=BBa_I751502
BBa_T5000	.	promoter	3516	3570	.	+	0	ID=annotation1955559;Name=plac;Parent=BBa_I751502
BBa_T5000	.	sequence_feature	3516	3522	.	+	0	ID=annotation1955560;Name=-35;Parent=BBa_I751502
BBa_T5000	.	non_covalent_binding_site	3523	3537	.	+	0	ID=annotation1955562;Name=O1;Parent=BBa_I751502
BBa_T5000	.	non_covalent_binding_site	3545	3570	.	+	0	ID=annotation1955563;Name=O1;Parent=BBa_I751502
BBa_T5000	.	ribosome_entry_site	1219	1230	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_T5000	.	conserved_region	1223	1226	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_T5000	.	terminator	4328	4456	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_T5000	.	terminator	4328	4407	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_T5000	.	engineered_region	4328	4407	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_T5000	.	stem_loop	4339	4382	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_T5000	.	terminator	4416	4456	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_T5000	.	stop_codon	4449	4449	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_T5000	.	polyA_site	4443	4456	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_T5000	.	stem_loop	4423	4442	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_T5000	.	engineered_region	4416	4456	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_T5000
ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcat
tttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtgg
tgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaa
aagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtga
tcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgcc
gcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttg
cccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccag
ttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgac
cgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcac
caccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataa
ccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcg
cttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactg
cgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaa
gccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacac
tttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttc
tccatacccgtttttttgggctagctactagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttat
acgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaa
aacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaa
cagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctc
gcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgca
caatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagct
gcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaag
acggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttc
tgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaa
ggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctgg
ttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggt
agtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctg
gggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcac
tggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagct
ggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctga
tagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttt
tatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatata
ctagagtttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatgaaaaacatc
aacgcggacgacacctaccgtatcattaacaaaatcaaagcgtgtcgttctaacaacgatattaaccaatgcctgtctg
atatgaccaaaatggttcactgtgaatattatctgctggccatcatttatccgcacagcatggtaaaatccgatatttc
tattctggataactacccaaaaaaatggcgtcagtactatgatgacgccaacctgatcaagtacgatccgatcgtagat
tactctaactctaaccattccccgattaactggaatatctttgagaacaacgcggttaacaaaaagtccccaaacgtta
tcaaagaagcaaaaaccagcggtctgatcaccggtttcagcttcccgattcataccgcgaacaacggcttcggtatgct
gtcttttgcgcactccgaaaaagacaactacatcgattctctgttcctgcacgcgtgcatgaacattcctctgatcgtt
ccgtccctggttgataactatcgcaagattaacattgccaataacaaaagcaacaacgacctgaccaaacgcgagaaag
aatgcctggcatgggcgtgcgaaggtaagtcctcttgggacatctccaaaattctgggttgctctgaacgcaccgtaac
tttccatctgaccaacgcgcagatgaaactgaacaccacgaatcgttgtcaatctatcagcaaagctatcctgaccggt
gcgatcgattgcccgtatttcaaaaactaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagac
tgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctt
tctgcgtttatatactagagacctgtaggatcgtacaggtttacttgtgagcggataacaatatagtgtgtggaattgt
gagcggataacaatttactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggt
gcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacc
tacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcg
gctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaagg
ctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgac
accctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtaca
actacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaa
catcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgccc
gacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagt
tcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaac
gaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactgg
ctcaccttcgggtgggcctttctgcgtttata