##gff-version 3
##sequence-region BBa_S05321 1 3448
BBa_S05321	.	promoter	1	55	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_S05321	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_S05321	.	promoter	20	25	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_S05321	.	engineered_region	1	55	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_S05321	.	promoter	42	47	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_S05321	.	start_codon	53	53	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_S05321	.	engineered_region	64	3448	.	+	0	ID=BBa_K274100;Name=BBa_K274100
BBa_S05321	.	ribosome_entry_site	994	1003	.	+	0	ID=BBa_J15001;Name=BBa_J15001;Parent=BBa_K274100
BBa_S05321	.	sequence_feature	994	996	.	+	0	ID=annotation1938045;Name=SacI;Parent=BBa_J15001
BBa_S05321	.	ribosome_entry_site	997	1003	.	+	0	ID=annotation1938046;Name=rbs;Parent=BBa_J15001
BBa_S05321	.	CDS	1010	1942	.	+	0	ID=BBa_K118002;Name=BBa_K118002;Parent=BBa_K274100
BBa_S05321	.	CDS	1010	1939	.	+	0	ID=annotation1995701;Name=crtB coding sequence;Parent=BBa_K118002
BBa_S05321	.	ribosome_entry_site	1951	1960	.	+	0	ID=BBa_J15001;Name=BBa_J15001;Parent=BBa_K274100
BBa_S05321	.	sequence_feature	1951	1953	.	+	0	ID=annotation1938045;Name=SacI;Parent=BBa_J15001
BBa_S05321	.	ribosome_entry_site	1954	1960	.	+	0	ID=annotation1938046;Name=rbs;Parent=BBa_J15001
BBa_S05321	.	CDS	1967	3448	.	+	0	ID=BBa_K118003;Name=BBa_K118003;Parent=BBa_K274100
BBa_S05321	.	CDS	1967	3445	.	+	0	ID=annotation1995702;Name=crtI coding sequence;Parent=BBa_K118003
BBa_S05321	.	point_mutation	2855	2860	.	+	0	ID=annotation1968245;Name=PstI site removed;Parent=BBa_K118003
BBa_S05321	.	point_mutation	2444	2449	.	+	0	ID=annotation1968244;Name=PstI site removed;Parent=BBa_K118003
BBa_S05321	.	CDS	74	985	.	+	0	ID=BBa_I742151;Name=BBa_I742151;Parent=BBa_K274100
BBa_S05321	.	ribosome_entry_site	64	67	.	+	0	ID=BBa_K118012;Name=BBa_K118012;Parent=BBa_K274100
>BBa_S05321
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagaggaggtactagatgacg
gtctgcgcaaaaaaacacgttcatctcactcgcgatgctgcggagcagttactggctgatattgatcgacgccttgatc
agttattgcccgtggagggagaacgggatgttgtgggtgccgcgatgcgtgaaggtgcgctggcaccgggaaaacgtat
tcgccccatgttgctgttgctgaccgcccgcgatctgggttgcgctgtcagccatgacggattactggatttggcctgt
gcggtggaaatggtccacgcggcttcgctgatccttgacgatatgccctgcatggacgatgcgaagctgcggcgcggac
gccctaccattcattctcattacggagagcatgtggcaatactggcggcggttgccttgctgagtaaagcctttggcgt
aattgccgatgcagatggcctcacgccgctggcaaaaaatcgggcggtttctgaactgtcaaacgccatcggcatgcaa
ggattggttcagggtcagttcaaggatctgtctgaaggggataagccgcgcagcgctgaagctattttgatgacgaatc
actttaaaaccagcacgctgttttgtgcctccatgcagatggcctcgattgttgcgaatgcctccagcgaagcgcgtga
ttgcctgcatcgtttttcacttgatcttggtcaggcatttcaactgctggacgatttgaccgatggcatgaccgacacc
ggtaaggatagcaatcaggacgccggtaaatcgacgctggtcaatctgttaggcccgagggcggttgaagaacgtctga
gacaacatcttcagcttgccagtgagcatctctctgcggcctgccaacacgggcacgccactcaacattttattcaggc
ctggtttgacaaaaaactcgctgccgtcagttaataatactagagctcaaggaggtactagatgaataatccgtcgtta
ctcaatcatgcggtcgaaacgatggcagttggctcgaaaagttttgcgacagcctcaaagttatttgatgcaaaaaccc
ggcgcagcgtactgatgctctacgcctggtgccgccattgtgacgatgttattgacgatcagacgctgggctttcaggc
ccggcagcctgccttacaaacgcccgaacaacgtctgatgcaacttgagatgaaaacgcgccaggcctatgcaggatcg
cagatgcacgaaccggcgtttgcggcttttcaggaagtggctatggctcatgatatcgccccggcttacgcgtttgatc
atctggaaggcttcgccatggatgtacgcgaagcgcaatacagccaactggatgatacgctgcgctattgctatcacgt
tgcaggcgttgtcggcttgatgatggcgcaaatcatgggcgtgcgggataacgccacgctggaccgcgcctgtgacctt
gggctggcatttcagttgaccaatattgctcgcgatattgtggacgatgcgcatgcgggccgctgttatctgccggcaa
gctggctggagcatgaaggtctgaacaaagagaattatgcggcacctgaaaaccgtcaggcgctgagccgtatcgcccg
tcgtttggtgcaggaagcagaaccttactatttgtctgccacagccggcctggcagggttgcccctgcgttccgcctgg
gcaatcgctacggcgaagcaggtttaccggaaaataggtgtcaaagttgaacaggccggtcagcaagcctgggatcagc
ggcagtcaacgaccacgcccgaaaaattaacgctgctgctggccgcctctggtcaggcccttacttcccggatgcgggc
tcatcctccccgccctgcgcatctctggcagcgcccgctctaataatactagagctcaaggaggtactagatgaaacca
actacggtaattggtgcaggcttcggtggcctggcactggcaattcgtctacaagctgcggggatccccgtcttactgc
ttgaacaacgtgataaacccggcggtcgggcttatgtctacgaggatcaggggtttacctttgatgcaggcccgacggt
tatcaccgatcccagtgccattgaagaactgtttgcactggcaggaaaacagttaaaagagtatgtcgaactgctgccg
gttacgccgttttaccgcctgtgttgggagtcagggaaggtctttaattacgataacgatcaaacccggctcgaagcgc
agattcagcagtttaatccccgcgatgtcgaaggttatcgtcagtttctggactattcacgcgcggtgtttaaagaagg
ctatctaaagctcggtactgtcccttttttatcgttcagagacatgcttcgcgccgcacctcaactggcgaaactgcaa
gcatggagaagcgtttacagtaaggttgccagttacatcgaagatgaacatctgcgccaggcgttttctttccactcgc
tgttggtgggcggcaatcccttcgccacctcatccatttatacgttgatacacgcgctggagcgtgagtggggcgtctg
gtttccgcgtggcggcaccggcgcattagttcaggggatgataaagctgtttcaggatctgggtggcgaagtcgtgtta
aacgccagagtcagccatatggaaacgacaggaaacaagattgaagccgtgcatttagaggacggtcgcaggttcctga
cgcaagccgtcgcgtcaaatgcagatgtggttcatacctatcgcgacctgttaagccagcaccctgccgcggttaagca
gtccaacaaactgcaaactaagcgcatgagtaactctctgtttgtgctctattttggtttgaatcaccatcatgatcag
ctcgcgcatcacacggtttgtttcggcccgcgttaccgcgagctgattgacgaaatttttaatcatgatggcctcgcag
aggacttctcactttatctgcacgcgccctgtgtcacggattcgtcactggcgcctgaaggttgcggcagttactatgt
gttggcgccggtgccgcatttaggcaccgcgaacctcgactggacggttgaggggccaaaactacgcgaccgtattttt
gcgtaccttgagcagcattacatgcctggcttacggagtcagctggtcacgcaccggatgtttacgccgtttgattttc
gcgaccagcttaatgcctatcatggctcagccttttctgtggagcccgttcttacccagagcgcctggtttcggccgca
taaccgcgataaaaccattactaatctctacctggtcggcgcaggcacgcatcccggcgcaggcattcctggcgtcatc
ggctcggcaaaagcgacagcaggtttgatgctggaggatctgatataataa