##gff-version 3
##sequence-region BBa_S05036 1 2275
BBa_S05036	.	engineered_region	209	220	.	+	0	ID=annotation2180681;Name=BBa_B0034
BBa_S05036	.	non_covalent_binding_site	1078	1119	.	+	0	ID=annotation2180693;Name=sigma 70
BBa_S05036	.	sequence_feature	1265	1278	.	+	0	ID=annotation2180695;Name=post-transcriptional regulation by CyaR
BBa_S05036	.	engineered_region	227	907	.	+	0	ID=annotation2180683;Name=BBa_E1010
BBa_S05036	.	promoter	1120	1177	.	+	0	ID=annotation2180694;Name=nadEp
BBa_S05036	.	stem_loop	2147	2226	.	+	0	ID=annotation2180697;Name=B0010
BBa_S05036	.	CDS	227	901	.	+	0	ID=annotation2180684;Name=mrfp1
BBa_S05036	.	stem_loop	2235	2275	.	+	0	ID=annotation2180698;Name=B0012
BBa_S05036	.	CDS	1276	2103	.	+	0	ID=annotation2180696;Name=NAD+ synthtase
BBa_S05036	.	start_codon	173	173	.	+	0	ID=annotation2180680;Name=start
BBa_S05036	.	promoter	137	142	.	+	0	ID=annotation2180677;Name=-35
BBa_S05036	.	engineered_region	1029	1069	.	+	0	ID=annotation2180688;Name=BBa_B0012
BBa_S05036	.	engineered_region	1	1069	.	+	0	ID=annotation2180692;Name=BBa_J04450
BBa_S05036	.	stem_loop	1036	1055	.	+	0	ID=annotation2180689;Name=T7 TE
BBa_S05036	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation2180676;Name=CAP
BBa_S05036	.	engineered_region	941	1020	.	+	0	ID=annotation2180686;Name=BBa_B0010
BBa_S05036	.	engineered_tag	908	932	.	+	0	ID=annotation2180685;Name=barcode
BBa_S05036	.	engineered_region	1078	2275	.	+	0	ID=annotation2180699;Name=BBa_K524003
BBa_S05036	.	polyA_site	1056	1069	.	+	0	ID=annotation2180690;Name=polya
BBa_S05036	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation2180679;Name=LacI
BBa_S05036	.	conserved_region	213	216	.	+	0	ID=annotation2180682;Name=conserved
BBa_S05036	.	stem_loop	952	995	.	+	0	ID=annotation2180687;Name=stem_loop
BBa_S05036	.	promoter	161	166	.	+	0	ID=annotation2180678;Name=-10
BBa_S05036	.	engineered_region	1	200	.	+	0	ID=annotation2180675;Name=BBa_R0010
BBa_S05036	.	stop_codon	1062	1062	.	+	0	ID=annotation2180691;Name=stop
>BBa_S05036
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcttcctc
cgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaa
ggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcg
cttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacct
gaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccag
gactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggtta
tgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaa
aatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcag
ctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacg
aacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcat
caaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactaga
gtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagccgtcctcttgtttatcagcgtgttagat
aagcctggaatacattgggcgctttttcaagcccgtgaacgaaacggctccgctttcagaggattcctgtatgacgttt
taaccaccattcagcccgctgtcgcttgtcgtttcagtagcaacgggttagctttaaggaagttttgtcttttctgtct
ggaggggttcaatgacattgcaacaacaaataataaaggcgctgggcgcaaaaccgcagattaatgctgaagaggaaat
tcgtcgtagtgtcgattttctgaaaagctacctgcaaacttatccgttcattaaatcactggtgctcgggatcagcggc
ggtcaggactccacgcttgccggaaagctgtgccagatggcgattaatgagctgcgcctggaaaccggcaacgaatcac
tgcaatttattgccgtacgcctgccctatggtgttcaggccgacgaacaagattgccaggatgccattgcctttattca
accggatcgcgtattaaccgttaatatcaagggcgcggtattggccagcgaacaggcattgcgggaagcaggcattgaa
ctgagcgattttgtccgtggcaatgaaaaagcgcgtgagcggatgaaagcacaatatagcattgcgggtatgaccagcg
gtgtcgtggtgggcaccgatcatgcagcagaagccattaccggattcttcactaaatatggtgacggcggtacggacat
taatccgctgtatcgtctcaacaaacgtcagggtaaacagttactggcggcattagcttgcccggaacacctttataag
aaagcgccaacggccgatctggaagatgatcgcccttctctgccagatgaagtggcactcggcgtgacctatgacaata
tcgacgactatctggaagggaaaaacgtacctcaacaggtcgccagaacaatagagaactggtatctgaaaaccgaaca
taaacgccgtccgccaattaccgttttcgatgatttctggaaaaagtaataatttgcacatattggattgtgcgaaaaa
gagtaatactagaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgt
cggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata