##gff-version 3
##sequence-region BBa_S04486 1 4519
BBa_S04486	.	engineered_region	1	3322	.	+	0	ID=BBa_S04471;Name=BBa_S04471
BBa_S04486	.	promoter	1	1309	.	+	0	ID=BBa_K091225;Name=BBa_K091225;Parent=BBa_S04471
BBa_S04486	.	promoter	1	130	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_K091225
BBa_S04486	.	engineered_region	139	1309	.	+	0	ID=BBa_S0100;Name=BBa_S0100;Parent=BBa_K091225
BBa_S04486	.	CDS	157	1284	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_S0100
BBa_S04486	.	engineered_tag	1285	1309	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_S04486	.	CDS	157	1284	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_S04486	.	engineered_region	157	1284	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_S04486	.	tag	1246	1284	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_S04486	.	ribosome_entry_site	139	150	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S0100
BBa_S04486	.	conserved_region	143	146	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04486	.	engineered_region	1318	3322	.	+	0	ID=BBa_K315009;Name=BBa_K315009;Parent=BBa_S04471
BBa_S04486	.	engineered_region	2114	3322	.	+	0	ID=BBa_J85100;Name=BBa_J85100;Parent=BBa_K315009
BBa_S04486	.	CDS	2132	3322	.	+	0	ID=BBa_J31007;Name=BBa_J31007;Parent=BBa_J85100
BBa_S04486	.	primer_binding_site	2132	2155	.	+	0	ID=annotation1910571;Name=Fwd primer J31007;Parent=BBa_J31007
BBa_S04486	.	primer_binding_site	3303	3322	.	+	0	ID=annotation1910572;Name=Rev primer J31007;Parent=BBa_J31007
BBa_S04486	.	CDS	2132	3322	.	+	0	ID=annotation1885000;Name=tetA(C) forward;Parent=BBa_J31007
BBa_S04486	.	stop_codon	3320	3322	.	+	0	ID=annotation1910573;Name=stop;Parent=BBa_J31007
BBa_S04486	.	ribosome_entry_site	2114	2125	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J85100
BBa_S04486	.	conserved_region	2118	2121	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04486	.	engineered_region	1318	2105	.	+	0	ID=BBa_K315008;Name=BBa_K315008;Parent=BBa_K315009
BBa_S04486	.	promoter	1318	1373	.	+	0	ID=BBa_K091110;Name=BBa_K091110;Parent=BBa_K315008
BBa_S04486	.	promoter	1334	1339	.	+	0	ID=annotation1964081;Name=-35;Parent=BBa_K091110
BBa_S04486	.	promoter	1361	1366	.	+	0	ID=annotation1964082;Name=-10;Parent=BBa_K091110
BBa_S04486	.	sequence_feature	1382	2105	.	+	0	ID=BBa_J70390;Name=BBa_J70390;Parent=BBa_K315008
BBa_S04486	.	ribosome_entry_site	1382	1393	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J70390
BBa_S04486	.	conserved_region	1386	1389	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04486	.	CDS	1400	2105	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_J70390
BBa_S04486	.	CDS	1400	2074	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_S04486	.	engineered_tag	2081	2105	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_S04486	.	CDS	3329	4519	.	+	0	ID=BBa_K315015;Name=BBa_K315015
>BBa_S04486
acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacg
ctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagat
ggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtg
aaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaacc
gcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtc
gcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggc
gtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatg
accaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccat
caacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatc
gcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatc
aaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatga
gggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccggg
ctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaacca
ccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaa
gggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgc
gcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaact
acgctttagtagcttaataactctgatagtgctagtgtagatctctactagagcgttgacaccatcgaatggcgcaaaa
cctttcgcggtatggcatgatagcgcccggtactagagaaagaggagaaatactagatggcttcctccgaagacgttat
caaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaa
ggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcc
tgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtcctt
cccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctg
caagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaa
ccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaa
actgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgct
tacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaag
gtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagaaagaggagaaatactagat
gaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggta
ctgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgt
tgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgct
acttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggcc
ggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccact
tcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctcctt
gcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcat
aagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcg
tcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcga
ggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagcc
ttcgtcactggccccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggct
acgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggat
gcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctctt
accagcctaacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttgg
catggattgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgac
ctaatactagatgaagagtaataatgccctaatagtcatactaggaacagtcacactagatgccgtcggaataggactc
gtcatgcccgtcctacccggactactaagggatatagtccatagtgatagtatagccagtcattatggagtcctgctag
cgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcct
gctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgccgga
cgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatc
gggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttggg
cgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatg
caggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggg
gcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggt
cattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgcc
ctcgctcaagccttcgtcactggccccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccg
acgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccgg
cggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcg
ctcgcggctcttaccagcctaacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacat
ggaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccg
ggccacctcgacctaa