##gff-version 3 ##sequence-region BBa_S04472 1 2625 BBa_S04472 . engineered_region 1 724 . + 0 ID=BBa_S04419;Name=BBa_S04419 BBa_S04472 . CDS 19 724 . + 0 ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_S04419 BBa_S04472 . engineered_tag 700 724 . + 0 ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010 BBa_S04472 . CDS 19 693 . + 0 ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010 BBa_S04472 . ribosome_entry_site 1 12 . + 0 ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S04419 BBa_S04472 . conserved_region 5 8 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_S04472 . engineered_region 733 2625 . + 0 ID=BBa_K199175;Name=BBa_K199175 BBa_S04472 . engineered_region 1482 2625 . + 0 ID=BBa_K109702;Name=BBa_K109702;Parent=BBa_K199175 BBa_S04472 . engineered_region 1690 2625 . + 0 ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_K109702 BBa_S04472 . CDS 1708 2463 . + 0 ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462 BBa_S04472 . engineered_tag 2464 2488 . + 0 ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062 BBa_S04472 . engineered_region 1708 2463 . + 0 ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062 BBa_S04472 . engineered_region 1708 1709 . + 0 ID=annotation1762;Name=prefix;Parent=BBa_C0062 BBa_S04472 . point_mutation 1881 1881 . + 0 ID=annotation1764;Name=T;Parent=BBa_C0062 BBa_S04472 . CDS 1708 2457 . + 0 ID=annotation1766;Name=luxR;Parent=BBa_C0062 BBa_S04472 . point_mutation 2199 2199 . + 0 ID=annotation1765;Name=A;Parent=BBa_C0062 BBa_S04472 . terminator 2585 2625 . + 0 ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462 BBa_S04472 . stop_codon 2618 2618 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_S04472 . polyA_site 2612 2625 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_S04472 . stem_loop 2592 2611 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_S04472 . engineered_region 2585 2625 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_S04472 . terminator 2497 2576 . + 0 ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462 BBa_S04472 . engineered_region 2497 2576 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_S04472 . stem_loop 2508 2551 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_S04472 . ribosome_entry_site 1690 1701 . + 0 ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462 BBa_S04472 . conserved_region 1694 1697 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_S04472 . promoter 1482 1681 . + 0 ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K109702 BBa_S04472 . non_covalent_binding_site 1570 1607 . + 0 ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010 BBa_S04472 . non_covalent_binding_site 1647 1681 . + 0 ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010 BBa_S04472 . promoter 1618 1623 . + 0 ID=annotation1961224;Name=-35;Parent=BBa_R0010 BBa_S04472 . engineered_region 1482 1681 . + 0 ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010 BBa_S04472 . promoter 1642 1647 . + 0 ID=annotation1961225;Name=-10;Parent=BBa_R0010 BBa_S04472 . start_codon 1654 1654 . + 0 ID=annotation1961227;Name=start;Parent=BBa_R0010 BBa_S04472 . CDS 1482 1569 . + 0 ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010 BBa_S04472 . engineered_region 733 1473 . + 0 ID=BBa_E5500;Name=BBa_E5500;Parent=BBa_K199175 BBa_S04472 . ribosome_entry_site 733 747 . + 0 ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_E5500 BBa_S04472 . engineered_region 733 747 . + 0 ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030 BBa_S04472 . ribosome_entry_site 740 744 . + 0 ID=annotation1702;Name=RBS;Parent=BBa_B0030 BBa_S04472 . ribosome_entry_site 733 747 . + 0 ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030 BBa_S04472 . CDS 754 1473 . + 0 ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E5500 BBa_S04472 . CDS 754 1473 . + 0 ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040 >BBa_S04472 aaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggtt ccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaa agttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgtt aaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcg aagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtgg taccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccg gaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaa ccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaa cgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgc tagtgtagatcgctactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcc caattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacata cggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggt tatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggtt atgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatac ccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaac tataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaaca ttgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccaga caaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagttt gtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagcaatacgcaaaccgcctctc cccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaa ttaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgt gagcggataacaatttcacacatactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacataca gaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtaca ttgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccct aaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcatt caccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatc aggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaa aaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataatt atcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatg cgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcg caaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatact ttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcg aaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtg ggcctttctgcgtttata