##gff-version 3
##sequence-region BBa_S04470 1 3466
BBa_S04470	.	promoter	1	1309	.	+	0	ID=BBa_K091225;Name=BBa_K091225
BBa_S04470	.	engineered_region	139	1309	.	+	0	ID=BBa_S0100;Name=BBa_S0100;Parent=BBa_K091225
BBa_S04470	.	CDS	157	1284	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_S0100
BBa_S04470	.	engineered_region	157	1284	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_S04470	.	CDS	157	1284	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_S04470	.	tag	1246	1284	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_S04470	.	engineered_tag	1285	1309	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_S04470	.	ribosome_entry_site	139	150	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S0100
BBa_S04470	.	conserved_region	143	146	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04470	.	promoter	1	130	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_K091225
BBa_S04470	.	engineered_region	1318	3466	.	+	0	ID=BBa_S04448;Name=BBa_S04448
BBa_S04470	.	CDS	2276	3466	.	+	0	ID=BBa_J31007;Name=BBa_J31007;Parent=BBa_S04448
BBa_S04470	.	CDS	2276	3466	.	+	0	ID=annotation1885000;Name=tetA(C) forward;Parent=BBa_J31007
BBa_S04470	.	primer_binding_site	2276	2299	.	+	0	ID=annotation1910571;Name=Fwd primer J31007;Parent=BBa_J31007
BBa_S04470	.	stop_codon	3464	3466	.	+	0	ID=annotation1910573;Name=stop;Parent=BBa_J31007
BBa_S04470	.	primer_binding_site	3447	3466	.	+	0	ID=annotation1910572;Name=Rev primer J31007;Parent=BBa_J31007
BBa_S04470	.	engineered_region	1318	2269	.	+	0	ID=BBa_S04446;Name=BBa_S04446;Parent=BBa_S04448
BBa_S04470	.	engineered_region	1318	2249	.	+	0	ID=BBa_I715039;Name=BBa_I715039;Parent=BBa_S04446
BBa_S04470	.	engineered_region	1526	2249	.	+	0	ID=BBa_I13502;Name=BBa_I13502;Parent=BBa_I715039
BBa_S04470	.	ribosome_entry_site	1526	1537	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13502
BBa_S04470	.	conserved_region	1530	1533	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04470	.	CDS	1544	2224	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13502
BBa_S04470	.	CDS	1544	2218	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_S04470	.	engineered_tag	2225	2249	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_S04470	.	promoter	1318	1517	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_I715039
BBa_S04470	.	CDS	1318	1405	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_S04470	.	promoter	1454	1459	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_S04470	.	promoter	1478	1483	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_S04470	.	start_codon	1490	1490	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_S04470	.	non_covalent_binding_site	1406	1443	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_S04470	.	engineered_region	1318	1517	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_S04470	.	non_covalent_binding_site	1483	1517	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_S04470	.	ribosome_entry_site	2258	2269	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S04446
BBa_S04470	.	conserved_region	2262	2265	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_S04470
acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacg
ctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagat
ggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtg
aaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaacc
gcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtc
gcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggc
gtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatg
accaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccat
caacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatc
gcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatc
aaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatga
gggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccggg
ctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaacca
ccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaa
gggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgc
gcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaact
acgctttagtagcttaataactctgatagtgctagtgtagatctctactagagcaatacgcaaaccgcctctccccgcg
cgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatg
tgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcgg
ataacaatttcacacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgc
gtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacga
aggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttc
cagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttca
aatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagtt
catctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaa
gcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtg
gtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacat
caaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccacc
ggtgcttaataacgctgatagtgctagtgtagatcgctactagagaaagaggagaaatactagatgaaatctaacaatg
cgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctctt
gcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttcta
tgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccacta
tcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatcaccggcgc
cacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagc
gcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattcc
ttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcg
accgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatg
actgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgct
ggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggccc
cgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcg
ttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcagg
ccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcctaacttc
gatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggc
gccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaa