##gff-version 3
##sequence-region BBa_S04449 1 2005
BBa_S04449	.	engineered_region	1	808	.	+	0	ID=BBa_S04447;Name=BBa_S04447
BBa_S04449	.	engineered_region	1	788	.	+	0	ID=BBa_K315008;Name=BBa_K315008;Parent=BBa_S04447
BBa_S04449	.	sequence_feature	65	788	.	+	0	ID=BBa_J70390;Name=BBa_J70390;Parent=BBa_K315008
BBa_S04449	.	CDS	83	788	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_J70390
BBa_S04449	.	CDS	83	757	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_S04449	.	engineered_tag	764	788	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_S04449	.	ribosome_entry_site	65	76	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J70390
BBa_S04449	.	conserved_region	69	72	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04449	.	promoter	1	56	.	+	0	ID=BBa_K091110;Name=BBa_K091110;Parent=BBa_K315008
BBa_S04449	.	promoter	44	49	.	+	0	ID=annotation1964082;Name=-10;Parent=BBa_K091110
BBa_S04449	.	promoter	17	22	.	+	0	ID=annotation1964081;Name=-35;Parent=BBa_K091110
BBa_S04449	.	ribosome_entry_site	797	808	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S04447
BBa_S04449	.	conserved_region	801	804	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04449	.	CDS	815	2005	.	+	0	ID=BBa_J31007;Name=BBa_J31007
BBa_S04449	.	CDS	815	2005	.	+	0	ID=annotation1885000;Name=tetA(C) forward;Parent=BBa_J31007
BBa_S04449	.	primer_binding_site	815	838	.	+	0	ID=annotation1910571;Name=Fwd primer J31007;Parent=BBa_J31007
BBa_S04449	.	stop_codon	2003	2005	.	+	0	ID=annotation1910573;Name=stop;Parent=BBa_J31007
BBa_S04449	.	primer_binding_site	1986	2005	.	+	0	ID=annotation1910572;Name=Rev primer J31007;Parent=BBa_J31007
>BBa_S04449
cgttgacaccatcgaatggcgcaaaacctttcgcggtatggcatgatagcgcccggtactagagaaagaggagaaatac
tagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacg
agttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtgg
tccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgac
atcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttg
ttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtc
cgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctg
aaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggcta
aaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccat
cgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgcta
ctagagaaagaggagaaatactagatgaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctg
taggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcacta
tggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggc
cgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtgga
tcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcac
cgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggcc
gggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgg
gctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctcctt
ccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccg
gcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcg
gaatcttgcacgccctcgctcaagccttcgtcactggccccgccaccaaacgtttcggcgagaagcaggccattatcgc
cggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgatt
cttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggac
agcttcaaggatcgctcgcggctcttaccagcctaacttcgatcattggaccgctgatcgtcacggcgatttatgccgc
ctcggcgagcacatggaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgc
ggtgcatggagccgggccacctcgacctaa