LOCUS       BBa_S04443              1442 bp    DNA     linear       12-Dec-2025
DEFINITION  J70575:J70554
ACCESSION   BBa_S04443
VERSION     BBa_S04443.1  
FEATURES             Location/Qualifiers
     misc_feature    1..4
                     /label=BsaXI partial site (only occurs when follows scar)
     misc_feature    795..805
                     /label=BsaXI site
     misc_feature    1040..1049
                     /label=BsaXI partial site (occurs with suffix or scar)
     misc_feature    506..506
                     /label=mutation from J70568: G506A (DNA), V169I (protein)
     promoter        1..170
                     /label=R0010(-naturally occurring internal BsaXI site)
     RBS             178..190
                     /label=B0034
     CDS             197..877
                     /label=E1010
     misc_feature    878..902
                     /label=Junk from J04450
     misc_feature    12..14
                     /label=BsaXI potential cut site
     misc_feature    783..785
                     /label=BsaXI cut site
     misc_feature    813..815
                     /label=BsaXI cut site
     misc_feature    1030..1032
                     /label=BsaXI cut site
     misc_feature    911..990
                     /label=B0010
     misc_feature    999..1039
                     /label=B0012
     misc_feature    1..1049
                     /label=BBa_J70575
     misc_feature    1056..1442
                     /label=BBa_J70554
ORIGIN
        1 ctccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
       61 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
      121 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca tactagagaa
      181 agaggagaaa tactagatgg cttcctccga agacgttatc aaagagttca tgcgtttcaa
      241 agttcgtatg gaaggttccg ttaacggtca cgagttcgaa atcgaaggtg aaggtgaagg
      301 tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt accaaaggtg gtccgctgcc
      361 gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt tccaaagctt acgttaaaca
      421 cccggctgac atcccggact acctgaaact gtccttcccg gaaggtttca aatgggaacg
      481 tgttatgaac ttcgaagacg gtggtattgt taccgttacc caggactcct ccctgcaaga
      541 cggtgagttc atctacaaag ttaaactgcg tggtaccaac ttcccgtccg acggtccggt
      601 tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa cgtatgtacc cggaagacgg
      661 tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa gacggtggtc actacgacgc
      721 tgaagttaaa accacctaca tggctaaaaa accggttcag ctgccgggtg cttacaaaac
      781 cgacatcaaa ctggacatca cctcccacaa cgaagactac accatcgttg aacagtacga
      841 acgtgctgaa ggtcgtcact ccaccggtgc ttaataacgc tgatagtgct agtgtagatc
      901 gctactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
      961 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
     1021 gggcctttct gcgtttatag gaggacaggt actagatgaa gaatggaatc aaagttaact
     1081 tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat tatcaacaaa
     1141 atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg tccacacaat
     1201 ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt gagtttgtaa
     1261 cagctgctgg gattacacat ggcatggatg aactatacaa ataataatac tagagccagg
     1321 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     1381 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
     1441 ta
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