##gff-version 3
##sequence-region BBa_S04441 1 837
BBa_S04441	.	promoter	764	837	.	+	0	ID=BBa_J13002;Name=BBa_J13002
BBa_S04441	.	ribosome_entry_site	826	837	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J13002
BBa_S04441	.	conserved_region	830	833	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04441	.	promoter	764	817	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_J13002
BBa_S04441	.	promoter	783	788	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_S04441	.	engineered_region	764	817	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_S04441	.	non_covalent_binding_site	764	782	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_S04441	.	non_covalent_binding_site	789	807	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_S04441	.	promoter	806	811	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_S04441	.	engineered_region	1	755	.	+	0	ID=BBa_K199118;Name=BBa_K199118
BBa_S04441	.	CDS	50	755	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K199118
BBa_S04441	.	engineered_tag	731	755	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_S04441	.	CDS	50	724	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_S04441	.	ribosome_entry_site	32	43	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K199118
BBa_S04441	.	conserved_region	36	39	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04441	.	promoter	1	23	.	+	0	ID=BBa_R0085;Name=BBa_R0085;Parent=BBa_K199118
BBa_S04441	.	sequence_feature	12	23	.	+	0	ID=annotation1721522;Name=Initiation Region;Parent=BBa_R0085
BBa_S04441	.	non_covalent_binding_site	1	11	.	+	0	ID=annotation1721521;Name=Polymerase Binding Region;Parent=BBa_R0085
BBa_S04441	.	sequence_feature	18	18	.	+	0	ID=annotation1721520;Name=Transcription Start Site;Parent=BBa_R0085
>BBa_S04441
taatacgactcactatagggagatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagag
ttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtc
cgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtcccc
gcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaa
ggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacg
gtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatggg
ttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaa
gacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaa
ccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtca
ctccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagtccctatcagtgatagagattgacatc
cctatcagtgatagagatactgagcactactagagaaagaggagaaa