##gff-version 3
##sequence-region BBa_S04440 1 989
BBa_S04440	.	terminator	779	858	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S04440	.	stem_loop	790	833	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04440	.	engineered_region	779	858	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04440	.	promoter	916	969	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_S04440	.	promoter	935	940	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_S04440	.	non_covalent_binding_site	941	959	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_S04440	.	promoter	958	963	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_S04440	.	non_covalent_binding_site	916	934	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_S04440	.	engineered_region	916	969	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_S04440	.	ribosome_entry_site	978	989	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S04440	.	conserved_region	982	985	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04440	.	terminator	867	907	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S04440	.	stop_codon	900	900	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04440	.	stem_loop	874	893	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04440	.	polyA_site	894	907	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04440	.	engineered_region	867	907	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04440	.	promoter	1	23	.	+	0	ID=BBa_R0085;Name=BBa_R0085
BBa_S04440	.	sequence_feature	18	18	.	+	0	ID=annotation1721520;Name=Transcription Start Site;Parent=BBa_R0085
BBa_S04440	.	sequence_feature	12	23	.	+	0	ID=annotation1721522;Name=Initiation Region;Parent=BBa_R0085
BBa_S04440	.	non_covalent_binding_site	1	11	.	+	0	ID=annotation1721521;Name=Polymerase Binding Region;Parent=BBa_R0085
BBa_S04440	.	ribosome_entry_site	32	44	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_S04440	.	engineered_region	32	44	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_S04440	.	ribosome_entry_site	32	44	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_S04440	.	ribosome_entry_site	38	41	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_S04440	.	CDS	51	770	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_S04440	.	CDS	51	770	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
>BBa_S04440
taatacgactcactatagggagatactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactgg
agttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgat
gcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcacta
ctttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcc
cgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaa
ggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattgg
aatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattag
acacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcctt
ttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttc
ttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaa
taaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtca
cactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatc
agtgatagagatactgagcactactagagaaagaggagaaa