##gff-version 3
##sequence-region BBa_S04369 1 2845
BBa_S04369	.	engineered_region	1	1646	.	+	0	ID=BBa_S04368;Name=BBa_S04368
BBa_S04369	.	engineered_region	1	1626	.	+	0	ID=BBa_S04366;Name=BBa_S04366;Parent=BBa_S04368
BBa_S04369	.	engineered_region	1	953	.	+	0	ID=BBa_S04365;Name=BBa_S04365;Parent=BBa_S04366
BBa_S04369	.	engineered_region	1	933	.	+	0	ID=BBa_S04364;Name=BBa_S04364;Parent=BBa_S04365
BBa_S04369	.	CDS	153	933	.	+	0	ID=BBa_C0079;Name=BBa_C0079;Parent=BBa_S04364
BBa_S04369	.	tag	870	902	.	+	0	ID=annotation307948;Name=LVA;Parent=BBa_C0079
BBa_S04369	.	sequence_alteration	632	632	.	+	0	ID=annotation308006;Name=G;Parent=BBa_C0079
BBa_S04369	.	engineered_tag	909	933	.	+	0	ID=annotation2213999;Name=Help:Barcodes;Parent=BBa_C0079
BBa_S04369	.	CDS	153	869	.	+	0	ID=annotation307935;Name=lasR;Parent=BBa_C0079
BBa_S04369	.	engineered_region	1	146	.	+	0	ID=BBa_S04363;Name=BBa_S04363;Parent=BBa_S04364
BBa_S04369	.	ribosome_entry_site	135	146	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S04363
BBa_S04369	.	conserved_region	139	142	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04369	.	promoter	1	126	.	+	0	ID=BBa_K091146;Name=BBa_K091146;Parent=BBa_S04363
BBa_S04369	.	non_covalent_binding_site	94	113	.	+	0	ID=annotation1967355;Name=LuxR;Parent=BBa_K091146
BBa_S04369	.	non_covalent_binding_site	75	93	.	+	0	ID=annotation1967354;Name=LasR;Parent=BBa_K091146
BBa_S04369	.	start_codon	125	125	.	+	0	ID=annotation1967358;Name=Transcriptional Start;Parent=BBa_K091146
BBa_S04369	.	sequence_feature	113	118	.	+	0	ID=annotation1967356;Name=-10;Parent=BBa_K091146
BBa_S04369	.	sequence_feature	86	91	.	+	0	ID=annotation1967357;Name=-35;Parent=BBa_K091146
BBa_S04369	.	ribosome_entry_site	942	953	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S04365
BBa_S04369	.	conserved_region	946	949	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04369	.	CDS	960	1626	.	+	0	ID=BBa_C0078;Name=BBa_C0078;Parent=BBa_S04366
BBa_S04369	.	CDS	960	1562	.	+	0	ID=annotation305970;Name=LasI;Parent=BBa_C0078
BBa_S04369	.	engineered_tag	1602	1626	.	+	0	ID=annotation2214003;Name=Help:Barcodes;Parent=BBa_C0078
BBa_S04369	.	tag	1563	1595	.	+	0	ID=annotation306608;Name=LVA;Parent=BBa_C0078
BBa_S04369	.	ribosome_entry_site	1635	1646	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S04368
BBa_S04369	.	conserved_region	1639	1642	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04369	.	CDS	1655	2845	.	+	0	ID=BBa_J31006;Name=BBa_J31006
BBa_S04369	.	CDS	1655	2845	.	+	0	ID=annotation1884998;Name=tetA(C) backwards;Parent=BBa_J31006
BBa_S04369	.	stop_codon	1655	1657	.	+	0	ID=annotation1910574;Name=stop;Parent=BBa_J31006
BBa_S04369	.	primer_binding_site	1655	1674	.	+	0	ID=annotation1910575;Name=Rev primer J31006;Parent=BBa_J31006
BBa_S04369	.	primer_binding_site	2822	2845	.	+	0	ID=annotation1910576;Name=Fwd primer J31006;Parent=BBa_J31006
>BBa_S04369
tgttctcgtgtgaagccattgctctgatcttttggacgtttcttcgagcctagcaagggtccgggttcaccgaaatcta
tctcatttgctagtacctgtaggatcgtacaggtataaattcttcagtactagagaaagaggagaaatactagatggcc
ttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcg
accttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaa
ctacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccag
agcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccg
ccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagc
ggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaa
agcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagt
ggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatggg
aaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctc
gctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagaaagagg
agaaatactagatgatcgttcagatcggtcgtcgtgaagagttcgacaaaaaactgctgggtgaaatgcacaaactgcg
tgctcaggttttcaaagaacgtaaaggttgggacgtttccgttatcgacgaaatggaaatcgacggttacgacgctctg
tccccgtactacatgctgatccaggaagacaccccggaagctcaggttttcggttgctggcgtatcttcgacaccaccg
gtccgtacatgctgaaaaacaccttcccggaactgctgcacggtaaagaagctccgtgctccccgcacatctgggaact
gtcccgtttcgctatcaactccggtcagaaaggttccctgggtttctccgactgcaccctggaagctatgcgtgctctg
gctcgttactccttgcagaacgacatccagaccctggttaccgttaccaccgttggtgttgaaaaaatgatgatccgtg
ctggtctggacgtttcccgtttcggtccgcacctgaaaatcggtatcgaacgtgctgttgctctgcgtatcgaactgaa
cgctaaaacccagatcgctctgtacggtggtgttctggttgaacagcgtctggctgtttccgctgctaacgacgaaaac
tacgctctggttgcttaataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactagagttagg
tcgaggtggcccggctccatgcaccgcgacgcaacgcggggaggcagacaaggtatagggcggcgcctacaatccatgc
caacccgttccatgtgctcgccgaggcggcataaatcgccgtgacgatcagcggtccaatgatcgaagttaggctggta
agagccgcgagcgatccttgaagctgtccctgatggtcgtcatctacctgcctggacagcatggcctgcaacgcgggca
tcccgatgccgccggaagcgagaagaatcataatggggaaggccatccagcctcgcgtcgcgaacgccagcaagacgta
gcccagcgcgtcggccgccatgccggcgataatggcctgcttctcgccgaaacgtttggtggcggggccagtgacgaag
gcttgagcgagggcgtgcaagattccgaataccgcaagcgacaggccgatcatcgtcgcgctccagcgaaagcggtcct
cgccgaaaatgacccagagcgctgccggcacctgtcctacgagttgcatgataaagaagacagtcataagtgcggcgac
gatagtcatgccccgcgcccaccggaaggagctgactgggttgaaggctctcaagggcatcggtcgacgctctccctta
tgcgactcctgcattaggaagcagcccagtagtaggttgaggccgttgagcaccgccgccgcaaggaatggtgcatgca
aggagatggcgcccaacagtcccccggccacggggcctgccaccatacccacgccgaaacaagcgctcatgagcccgaa
gtggcgagcccgatcttccccatcggtgatgtcggcgatataggcgccagcaaccgcacctgtggcgccggtgatgccg
gccacgatgcgtccggcgtagaggatccacaggacgggtgtggtcgccatgatcgcgtagtcgatagtggctccaagta
gcgaagcgagcaggactgggcggcggccaaagcggtcggacagtgctccgagaacgggtgcgcatagaaattgcatcaa
cgcatatagcgctagcagcacgccatagtgactggcgatgctgtcggaatggacgatatcccgcaagaggcccggcagt
accggcataaccaagcctatgcctacagcatccagggtgacggtgccgaggatgacgatgagcgcattgttagatttcat
t