##gff-version 3
##sequence-region BBa_S04360 1 3693
BBa_S04360	.	engineered_region	790	3693	.	+	0	ID=BBa_S04359;Name=BBa_S04359
BBa_S04360	.	engineered_region	808	3693	.	+	0	ID=BBa_S04358;Name=BBa_S04358;Parent=BBa_S04359
BBa_S04360	.	CDS	808	1563	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_S04358
BBa_S04360	.	point_mutation	1299	1299	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_S04360	.	CDS	808	1557	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_S04360	.	engineered_region	808	1563	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_S04360	.	engineered_tag	1564	1588	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_S04360	.	point_mutation	981	981	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_S04360	.	engineered_region	808	809	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_S04360	.	engineered_region	1597	3693	.	+	0	ID=BBa_S04357;Name=BBa_S04357;Parent=BBa_S04358
BBa_S04360	.	terminator	3565	3644	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_S04357
BBa_S04360	.	engineered_region	3565	3644	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04360	.	stem_loop	3576	3619	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04360	.	CDS	1617	2807	.	+	0	ID=BBa_J31006;Name=BBa_J31006;Parent=BBa_S04357
BBa_S04360	.	CDS	1617	2807	.	+	0	ID=annotation1884998;Name=tetA(C) backwards;Parent=BBa_J31006
BBa_S04360	.	stop_codon	1617	1619	.	+	0	ID=annotation1910574;Name=stop;Parent=BBa_J31006
BBa_S04360	.	primer_binding_site	1617	1636	.	+	0	ID=annotation1910575;Name=Rev primer J31006;Parent=BBa_J31006
BBa_S04360	.	primer_binding_site	2784	2807	.	+	0	ID=annotation1910576;Name=Fwd primer J31006;Parent=BBa_J31006
BBa_S04360	.	CDS	2834	3556	.	+	0	ID=BBa_E0020;Name=BBa_E0020;Parent=BBa_S04357
BBa_S04360	.	ribosome_entry_site	1597	1608	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S04357
BBa_S04360	.	conserved_region	1601	1604	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04360	.	terminator	3653	3693	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_S04357
BBa_S04360	.	engineered_region	3653	3693	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04360	.	stop_codon	3686	3686	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04360	.	stem_loop	3660	3679	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04360	.	polyA_site	3680	3693	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04360	.	ribosome_entry_site	2816	2827	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S04357
BBa_S04360	.	conserved_region	2820	2823	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04360	.	ribosome_entry_site	790	801	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S04359
BBa_S04360	.	conserved_region	794	797	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04360	.	CDS	1	756	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_S04360	.	point_mutation	492	492	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_S04360	.	CDS	1	750	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_S04360	.	engineered_region	1	756	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_S04360	.	engineered_tag	757	781	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_S04360	.	point_mutation	174	174	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_S04360	.	engineered_region	1	2	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
>BBa_S04360
atgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatc
aatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaa
atctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgat
cctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaat
ctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatgg
cttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacata
ccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaacca
aaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtga
gcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagca
attttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagaga
aagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaag
caataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttat
cctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgcta
atttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaa
tgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctatt
catacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttac
atgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatc
aaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaa
atattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgcc
aaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtg
tagatcactactagagaaagaggagaaatactagagttaggtcgaggtggcccggctccatgcaccgcgacgcaacgcg
gggaggcagacaaggtatagggcggcgcctacaatccatgccaacccgttccatgtgctcgccgaggcggcataaatcg
ccgtgacgatcagcggtccaatgatcgaagttaggctggtaagagccgcgagcgatccttgaagctgtccctgatggtc
gtcatctacctgcctggacagcatggcctgcaacgcgggcatcccgatgccgccggaagcgagaagaatcataatgggg
aaggccatccagcctcgcgtcgcgaacgccagcaagacgtagcccagcgcgtcggccgccatgccggcgataatggcct
gcttctcgccgaaacgtttggtggcggggccagtgacgaaggcttgagcgagggcgtgcaagattccgaataccgcaag
cgacaggccgatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgctgccggcacctgtcct
acgagttgcatgataaagaagacagtcataagtgcggcgacgatagtcatgccccgcgcccaccggaaggagctgactg
ggttgaaggctctcaagggcatcggtcgacgctctcccttatgcgactcctgcattaggaagcagcccagtagtaggtt
gaggccgttgagcaccgccgccgcaaggaatggtgcatgcaaggagatggcgcccaacagtcccccggccacggggcct
gccaccatacccacgccgaaacaagcgctcatgagcccgaagtggcgagcccgatcttccccatcggtgatgtcggcga
tataggcgccagcaaccgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagaggatccacaggacggg
tgtggtcgccatgatcgcgtagtcgatagtggctccaagtagcgaagcgagcaggactgggcggcggccaaagcggtcg
gacagtgctccgagaacgggtgcgcatagaaattgcatcaacgcatatagcgctagcagcacgccatagtgactggcga
tgctgtcggaatggacgatatcccgcaagaggcccggcagtaccggcataaccaagcctatgcctacagcatccagggt
gacggtgccgaggatgacgatgagcgcattgttagatttcattactagagaaagaggagaaatactagatggtgagcaa
gggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtg
tccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgc
cctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacga
cttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacc
cgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggca
acatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcat
caaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacaccccc
atcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgaga
agcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaata
atactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggt
gaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata