##gff-version 3
##sequence-region BBa_S04307 1 2157
BBa_S04307	.	promoter	1	1309	.	+	0	ID=BBa_K091225;Name=BBa_K091225
BBa_S04307	.	engineered_region	139	1309	.	+	0	ID=BBa_S0100;Name=BBa_S0100;Parent=BBa_K091225
BBa_S04307	.	CDS	157	1284	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_S0100
BBa_S04307	.	engineered_tag	1285	1309	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_S04307	.	CDS	157	1284	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_S04307	.	tag	1246	1284	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_S04307	.	engineered_region	157	1284	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_S04307	.	ribosome_entry_site	139	150	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S0100
BBa_S04307	.	conserved_region	143	146	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04307	.	promoter	1	130	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_K091225
BBa_S04307	.	engineered_region	1318	2157	.	+	0	ID=BBa_P0440;Name=BBa_P0440
BBa_S04307	.	CDS	1336	1995	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_P0440
BBa_S04307	.	engineered_tag	1996	2020	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_S04307	.	CDS	1339	1955	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_S04307	.	tag	1956	1989	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_S04307	.	terminator	2029	2108	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0440
BBa_S04307	.	engineered_region	2029	2108	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04307	.	stem_loop	2040	2083	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04307	.	ribosome_entry_site	1318	1329	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0440
BBa_S04307	.	conserved_region	1322	1325	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04307	.	terminator	2117	2157	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0440
BBa_S04307	.	engineered_region	2117	2157	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04307	.	stem_loop	2124	2143	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04307	.	stop_codon	2150	2150	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04307	.	polyA_site	2144	2157	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
>BBa_S04307
acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacg
ctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagat
ggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtg
aaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaacc
gcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtc
gcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggc
gtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatg
accaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccat
caacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatc
gcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatc
aaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatga
gggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccggg
ctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaacca
ccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaa
gggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgc
gcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaact
acgctttagtagcttaataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactagatgtccag
attagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaa
ctcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttag
ccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataa
cgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaa
cagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactca
gcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacc
tactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttctta
ttcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaact
acgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggct
cagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacctt
cgggtgggcctttctgcgtttata