##gff-version 3
##sequence-region BBa_S04303 1 2315
BBa_S04303	.	engineered_region	1	1391	.	+	0	ID=BBa_S04302;Name=BBa_S04302
BBa_S04303	.	engineered_region	1	1371	.	+	0	ID=BBa_Q04121;Name=BBa_Q04121;Parent=BBa_S04302
BBa_S04303	.	terminator	1180	1308	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_Q04121
BBa_S04303	.	terminator	1268	1308	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_S04303	.	engineered_region	1268	1308	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04303	.	stem_loop	1275	1294	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04303	.	stop_codon	1301	1301	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04303	.	polyA_site	1295	1308	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04303	.	terminator	1180	1259	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_S04303	.	engineered_region	1180	1259	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04303	.	stem_loop	1191	1234	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04303	.	promoter	1317	1370	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_Q04121
BBa_S04303	.	operator	1342	1358	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_S04303	.	operator	1319	1335	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_S04303	.	promoter	1359	1364	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_S04303	.	engineered_region	1317	1370	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_S04303	.	promoter	1336	1341	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_S04303	.	CDS	19	1146	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_Q04121
BBa_S04303	.	engineered_tag	1147	1171	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_S04303	.	CDS	19	1146	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_S04303	.	tag	1108	1146	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_S04303	.	engineered_region	19	1146	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_S04303	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04121
BBa_S04303	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04303	.	ribosome_entry_site	1380	1391	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S04302
BBa_S04303	.	conserved_region	1384	1387	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04303	.	CDS	1398	2315	.	+	0	ID=BBa_S04301;Name=BBa_S04301
BBa_S04303	.	terminator	2187	2315	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_S04301
BBa_S04303	.	terminator	2275	2315	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_S04303	.	engineered_region	2275	2315	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04303	.	stem_loop	2282	2301	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04303	.	stop_codon	2308	2308	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04303	.	polyA_site	2302	2315	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04303	.	terminator	2187	2266	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_S04303	.	engineered_region	2187	2266	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04303	.	stem_loop	2198	2241	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04303	.	CDS	1398	2178	.	+	0	ID=BBa_C0079;Name=BBa_C0079;Parent=BBa_S04301
BBa_S04303	.	sequence_alteration	1877	1877	.	+	0	ID=annotation308006;Name=G;Parent=BBa_C0079
BBa_S04303	.	CDS	1398	2114	.	+	0	ID=annotation307935;Name=lasR;Parent=BBa_C0079
BBa_S04303	.	engineered_tag	2154	2178	.	+	0	ID=annotation2213999;Name=Help:Barcodes;Parent=BBa_C0079
BBa_S04303	.	tag	2115	2147	.	+	0	ID=annotation307948;Name=LVA;Parent=BBa_C0079
>BBa_S04303
aaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatc
agaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcgga
gctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagt
ctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgt
cgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgat
cattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgat
gtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcg
cattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggca
taaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaa
accatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgc
gcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatg
ttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactc
tctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaata
cgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggca
ggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggc
atcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctacta
gagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacatt
gtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatggccttggttgacggttttcttg
agctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattctcgaagat
cctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgcctggcgc
gagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccgattttct
gggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgtatgggct
gaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggccgaggcc
aaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccggactggcct
tcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcggcaagac
cagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcggaagttc
ggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctcgctgcaaacgacgaaaact
acgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggct
cagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacctt
cgggtgggcctttctgcgtttata