##gff-version 3
##sequence-region BBa_S04253 1 1971
BBa_S04253	.	engineered_region	1	992	.	+	0	ID=BBa_J01101;Name=BBa_J01101
BBa_S04253	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J01101
BBa_S04253	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04253	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_J01101
BBa_S04253	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_S04253	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_S04253	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_S04253	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_S04253	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_S04253	.	CDS	81	830	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_J01101
BBa_S04253	.	engineered_tag	831	855	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_S04253	.	CDS	84	791	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_S04253	.	tag	792	824	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_S04253	.	terminator	952	992	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J01101
BBa_S04253	.	stop_codon	985	985	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04253	.	polyA_site	979	992	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04253	.	stem_loop	959	978	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04253	.	engineered_region	952	992	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04253	.	terminator	864	943	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J01101
BBa_S04253	.	engineered_region	864	943	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04253	.	stem_loop	875	918	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04253	.	engineered_region	1001	1971	.	+	0	ID=BBa_I763011;Name=BBa_I763011
BBa_S04253	.	terminator	1843	1922	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I763011
BBa_S04253	.	engineered_region	1843	1922	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04253	.	stem_loop	1854	1897	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04253	.	engineered_region	1076	1834	.	+	0	ID=BBa_J04031;Name=BBa_J04031;Parent=BBa_I763011
BBa_S04253	.	stop_codon	1829	1834	.	+	0	ID=annotation1507242;Name=Double Stop;Parent=BBa_J04031
BBa_S04253	.	start_codon	1076	1078	.	+	0	ID=annotation1507246;Name=Start;Parent=BBa_J04031
BBa_S04253	.	tag	1790	1828	.	+	0	ID=annotation1507235;Name=LVA;Parent=BBa_J04031
BBa_S04253	.	engineered_region	1076	1795	.	+	0	ID=annotation1507202;Name=BBa_E0040;Parent=BBa_J04031
BBa_S04253	.	ribosome_entry_site	1058	1069	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I763011
BBa_S04253	.	conserved_region	1062	1065	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04253	.	promoter	1001	1049	.	+	0	ID=BBa_R0051;Name=BBa_R0051;Parent=BBa_I763011
BBa_S04253	.	engineered_region	1001	1049	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_S04253	.	promoter	1038	1043	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_S04253	.	non_covalent_binding_site	1025	1041	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_S04253	.	promoter	1015	1020	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_S04253	.	non_covalent_binding_site	1001	1017	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_S04253	.	terminator	1931	1971	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I763011
BBa_S04253	.	stop_codon	1964	1964	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04253	.	polyA_site	1958	1971	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04253	.	stem_loop	1938	1957	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04253	.	engineered_region	1931	1971	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_S04253
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaag
aaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttattta
atggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagccc
ttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccct
gttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaa
gcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaa
gccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccaga
cttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagt
acccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttgg
cgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggc
atcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctacta
gagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaacaccgtgcgtgttgactattttac
ctctggcggtgataatggttgctactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggag
ttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgc
aacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactact
ttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccg
aaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaagg
tgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaa
tacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagac
acaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcctttt
accagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttctt
gagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacg
ctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttta
tctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata