##gff-version 3
##sequence-region BBa_S04221 1 1274
BBa_S04221	.	engineered_region	58	1274	.	+	0	ID=BBa_S04214;Name=BBa_S04214
BBa_S04221	.	engineered_region	807	1274	.	+	0	ID=BBa_K091137;Name=BBa_K091137;Parent=BBa_S04214
BBa_S04221	.	terminator	1146	1274	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K091137
BBa_S04221	.	terminator	1146	1225	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_S04221	.	engineered_region	1146	1225	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04221	.	stem_loop	1157	1200	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04221	.	terminator	1234	1274	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_S04221	.	stop_codon	1267	1267	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04221	.	polyA_site	1261	1274	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04221	.	stem_loop	1241	1260	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04221	.	engineered_region	1234	1274	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04221	.	engineered_region	807	1137	.	+	0	ID=BBa_I13025;Name=BBa_I13025;Parent=BBa_K091137
BBa_S04221	.	ribosome_entry_site	807	818	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13025
BBa_S04221	.	conserved_region	811	814	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04221	.	CDS	825	1112	.	+	0	ID=BBa_C0072;Name=BBa_C0072;Parent=BBa_I13025
BBa_S04221	.	tag	1074	1106	.	+	0	ID=annotation308503;Name=LVA;Parent=BBa_C0072
BBa_S04221	.	point_mutation	919	921	.	+	0	ID=annotation308502;Name=T;Parent=BBa_C0072
BBa_S04221	.	CDS	825	1073	.	+	0	ID=annotation308499;Name=mnt;Parent=BBa_C0072
BBa_S04221	.	engineered_tag	1113	1137	.	+	0	ID=annotation2214010;Name=Help:Barcodes;Parent=BBa_C0072
BBa_S04221	.	engineered_region	58	798	.	+	0	ID=BBa_E5500;Name=BBa_E5500;Parent=BBa_S04214
BBa_S04221	.	ribosome_entry_site	58	72	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_E5500
BBa_S04221	.	ribosome_entry_site	65	69	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_S04221	.	engineered_region	58	72	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_S04221	.	ribosome_entry_site	58	72	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_S04221	.	CDS	79	798	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E5500
BBa_S04221	.	CDS	79	798	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_S04221	.	promoter	1	49	.	+	0	ID=BBa_R0051;Name=BBa_R0051
BBa_S04221	.	engineered_region	1	49	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_S04221	.	promoter	38	43	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_S04221	.	non_covalent_binding_site	25	41	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_S04221	.	promoter	15	20	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_S04221	.	non_covalent_binding_site	1	17	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
>BBa_S04221
taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagattaaagaggagaaatactaga
tgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaatt
ttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaacta
cctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaac
agcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaacta
caagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaa
gatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaaga
atggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaa
tactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatccc
aacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactataca
aataataatactagagaaagaggagaaatactagatggcccgggatgatcctcacttcaattttcgtatgccaatggaa
gtaagagagaaattgaaatttagagcagaggcaaacggacggagcatgaactctgagcttttgcaaatcgtacaagatg
ccctaagcaaaccgtcaccagtcactgggtaccgcaatgatgcggaacgactcgccgatgagcagagcgagttagtgaa
gaagatggtcttcgatacactgaaggatctttataaaaaaaccaccgctgcaaacgacgaaaactacgctttagtagct
taataaccctgatagtgctagtgtagatccctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactg
ggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttc
tgcgtttata