##gff-version 3
##sequence-region BBa_S04220 1 3391
BBa_S04220	.	engineered_region	1	883	.	+	0	ID=BBa_I739001;Name=BBa_I739001
BBa_S04220	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I739001
BBa_S04220	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04220	.	CDS	62	746	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_I739001
BBa_S04220	.	CDS	65	681	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_S04220	.	engineered_tag	722	746	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_S04220	.	tag	682	715	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_S04220	.	promoter	1	35	.	+	0	ID=BBa_J23105;Name=BBa_J23105;Parent=BBa_I739001
BBa_S04220	.	terminator	755	883	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_I739001
BBa_S04220	.	terminator	843	883	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_S04220	.	stop_codon	876	876	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04220	.	polyA_site	870	883	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04220	.	stem_loop	850	869	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04220	.	engineered_region	843	883	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04220	.	terminator	755	834	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_S04220	.	engineered_region	755	834	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04220	.	stem_loop	766	809	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04220	.	engineered_region	892	3391	.	+	0	ID=BBa_I763027;Name=BBa_I763027
BBa_S04220	.	engineered_region	2270	3391	.	+	0	ID=BBa_I763004;Name=BBa_I763004;Parent=BBa_I763027
BBa_S04220	.	terminator	3263	3342	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I763004
BBa_S04220	.	engineered_region	3263	3342	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04220	.	stem_loop	3274	3317	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04220	.	terminator	3351	3391	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I763004
BBa_S04220	.	stop_codon	3384	3384	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04220	.	polyA_site	3378	3391	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04220	.	stem_loop	3358	3377	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04220	.	engineered_region	3351	3391	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04220	.	engineered_region	2496	3254	.	+	0	ID=BBa_J04031;Name=BBa_J04031;Parent=BBa_I763004
BBa_S04220	.	stop_codon	3249	3254	.	+	0	ID=annotation1507242;Name=Double Stop;Parent=BBa_J04031
BBa_S04220	.	tag	3210	3248	.	+	0	ID=annotation1507235;Name=LVA;Parent=BBa_J04031
BBa_S04220	.	engineered_region	2496	3215	.	+	0	ID=annotation1507202;Name=BBa_E0040;Parent=BBa_J04031
BBa_S04220	.	start_codon	2496	2498	.	+	0	ID=annotation1507246;Name=Start;Parent=BBa_J04031
BBa_S04220	.	ribosome_entry_site	2478	2489	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I763004
BBa_S04220	.	conserved_region	2482	2485	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04220	.	promoter	2270	2469	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_I763004
BBa_S04220	.	promoter	2430	2435	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_S04220	.	non_covalent_binding_site	2358	2395	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_S04220	.	promoter	2406	2411	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_S04220	.	start_codon	2442	2442	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_S04220	.	engineered_region	2270	2469	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_S04220	.	non_covalent_binding_site	2435	2469	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_S04220	.	CDS	2270	2357	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_S04220	.	engineered_region	892	2261	.	+	0	ID=BBa_I763026;Name=BBa_I763026;Parent=BBa_I763027
BBa_S04220	.	terminator	2133	2261	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_I763026
BBa_S04220	.	terminator	2221	2261	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_S04220	.	stop_codon	2254	2254	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04220	.	polyA_site	2248	2261	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04220	.	stem_loop	2228	2247	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04220	.	engineered_region	2221	2261	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04220	.	terminator	2133	2212	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_S04220	.	engineered_region	2133	2212	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04220	.	stem_loop	2144	2187	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04220	.	engineered_region	892	2124	.	+	0	ID=BBa_I763025;Name=BBa_I763025;Parent=BBa_I763026
BBa_S04220	.	promoter	892	945	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_I763025
BBa_S04220	.	non_covalent_binding_site	917	935	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_S04220	.	promoter	934	939	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_S04220	.	engineered_region	892	945	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_S04220	.	non_covalent_binding_site	892	910	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_S04220	.	promoter	911	916	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_S04220	.	engineered_region	954	2124	.	+	0	ID=BBa_S0100;Name=BBa_S0100;Parent=BBa_I763025
BBa_S04220	.	ribosome_entry_site	954	965	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S0100
BBa_S04220	.	conserved_region	958	961	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04220	.	CDS	972	2099	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_S0100
BBa_S04220	.	engineered_tag	2100	2124	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_S04220	.	engineered_region	972	2099	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_S04220	.	tag	2061	2099	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_S04220	.	CDS	972	2099	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
>BBa_S04220
tttacggctagctcagtcctaggtactatgctagctactagagaaagaggagaaatactagatgtccagattagataaa
agtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccaga
agctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagat
gttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagt
tttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaa
ctctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggg
gcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgat
agtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttg
aattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagt
agcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaag
actgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcc
tttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactac
tagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctc
ttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatg
gcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacct
ccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggt
ggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtggg
ctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttc
ttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatct
ggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggc
tggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttc
aacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgc
aatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagc
tcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgc
aactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcc
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagc
caggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
tactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccc
cgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaatt
aatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtga
gcggataacaatttcacacatactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagtt
gtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaa
catacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactacttt
cggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaa
ggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtg
atacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaata
caactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacac
aacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttac
cagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttga
gtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacgct
ttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatc
tgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata