##gff-version 3
##sequence-region BBa_S04214 1 1217
BBa_S04214	.	engineered_region	750	1217	.	+	0	ID=BBa_K091137;Name=BBa_K091137
BBa_S04214	.	terminator	1089	1217	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K091137
BBa_S04214	.	terminator	1177	1217	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_S04214	.	stem_loop	1184	1203	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04214	.	engineered_region	1177	1217	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04214	.	stop_codon	1210	1210	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04214	.	polyA_site	1204	1217	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04214	.	terminator	1089	1168	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_S04214	.	engineered_region	1089	1168	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04214	.	stem_loop	1100	1143	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04214	.	engineered_region	750	1080	.	+	0	ID=BBa_I13025;Name=BBa_I13025;Parent=BBa_K091137
BBa_S04214	.	CDS	768	1055	.	+	0	ID=BBa_C0072;Name=BBa_C0072;Parent=BBa_I13025
BBa_S04214	.	tag	1017	1049	.	+	0	ID=annotation308503;Name=LVA;Parent=BBa_C0072
BBa_S04214	.	point_mutation	862	864	.	+	0	ID=annotation308502;Name=T;Parent=BBa_C0072
BBa_S04214	.	engineered_tag	1056	1080	.	+	0	ID=annotation2214010;Name=Help:Barcodes;Parent=BBa_C0072
BBa_S04214	.	CDS	768	1016	.	+	0	ID=annotation308499;Name=mnt;Parent=BBa_C0072
BBa_S04214	.	ribosome_entry_site	750	761	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13025
BBa_S04214	.	conserved_region	754	757	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04214	.	engineered_region	1	741	.	+	0	ID=BBa_E5500;Name=BBa_E5500
BBa_S04214	.	ribosome_entry_site	1	15	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_E5500
BBa_S04214	.	engineered_region	1	15	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_S04214	.	ribosome_entry_site	8	12	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_S04214	.	ribosome_entry_site	1	15	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_S04214	.	CDS	22	741	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E5500
BBa_S04214	.	CDS	22	741	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
>BBa_S04214
attaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatg
gtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatt
tatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcg
agatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatat
ttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagtt
aaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatac
atcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaac
tagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacaca
atctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattaca
catggcatggatgaactatacaaataataatactagagaaagaggagaaatactagatggcccgggatgatcctcactt
caattttcgtatgccaatggaagtaagagagaaattgaaatttagagcagaggcaaacggacggagcatgaactctgag
cttttgcaaatcgtacaagatgccctaagcaaaccgtcaccagtcactgggtaccgcaatgatgcggaacgactcgccg
atgagcagagcgagttagtgaagaagatggtcttcgatacactgaaggatctttataaaaaaaccaccgctgcaaacga
cgaaaactacgctttagtagcttaataaccctgatagtgctagtgtagatccctactagagccaggcatcaaataaaac
gaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactgg
ctcaccttcgggtgggcctttctgcgtttata