##gff-version 3
##sequence-region BBa_S04190 1 1533
BBa_S04190	.	engineered_region	1	587	.	+	0	ID=BBa_I6413;Name=BBa_I6413
BBa_S04190	.	promoter	1	45	.	+	0	ID=BBa_R2000;Name=BBa_R2000;Parent=BBa_I6413
BBa_S04190	.	start_codon	45	45	.	+	0	ID=annotation318102;Name=(+1);Parent=BBa_R2000
BBa_S04190	.	sequence_feature	1	9	.	+	0	ID=annotation318100;Name=consensus;Parent=BBa_R2000
BBa_S04190	.	promoter	33	38	.	+	0	ID=annotation318098;Name=-10;Parent=BBa_R2000
BBa_S04190	.	promoter	10	15	.	+	0	ID=annotation318094;Name=-35;Parent=BBa_R2000
BBa_S04190	.	non_covalent_binding_site	20	33	.	+	0	ID=annotation318099;Name=Zif23;Parent=BBa_R2000
BBa_S04190	.	engineered_region	54	587	.	+	0	ID=BBa_E0435;Name=BBa_E0435;Parent=BBa_I6413
BBa_S04190	.	engineered_region	72	450	.	+	0	ID=BBa_E0035;Name=BBa_E0035;Parent=BBa_E0435
BBa_S04190	.	sequence_feature	270	419	.	+	0	ID=annotation1959044;Name=lacZ gene fragment;Parent=BBa_E0035
BBa_S04190	.	sequence_alteration	419	419	.	+	0	ID=annotation1959045;Name=inserted T;Parent=BBa_E0035
BBa_S04190	.	promoter	244	262	.	+	0	ID=annotation1959038;Name=T7 promoter;Parent=BBa_E0035
BBa_S04190	.	sequence_feature	72	86	.	+	0	ID=annotation1959035;Name=lacZ gene fragment;Parent=BBa_E0035
BBa_S04190	.	CDS	72	425	.	+	0	ID=annotation1959036;Name=lacZ alpha;Parent=BBa_E0035
BBa_S04190	.	sequence_alteration	179	179	.	+	0	ID=annotation1959042;Name=C;Parent=BBa_E0035
BBa_S04190	.	sequence_alteration	149	149	.	+	0	ID=annotation1959039;Name=C;Parent=BBa_E0035
BBa_S04190	.	promoter	97	106	.	+	0	ID=annotation1959037;Name=T3 promoter;Parent=BBa_E0035
BBa_S04190	.	sequence_alteration	182	182	.	+	0	ID=annotation1959043;Name=G;Parent=BBa_E0035
BBa_S04190	.	engineered_tag	426	450	.	+	0	ID=annotation1959046;Name=barcode;Parent=BBa_E0035
BBa_S04190	.	sequence_alteration	152	152	.	+	0	ID=annotation1959040;Name=T;Parent=BBa_E0035
BBa_S04190	.	sequence_alteration	158	158	.	+	0	ID=annotation1959041;Name=A;Parent=BBa_E0035
BBa_S04190	.	ribosome_entry_site	54	65	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0435
BBa_S04190	.	conserved_region	58	61	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04190	.	terminator	459	587	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_E0435
BBa_S04190	.	terminator	459	538	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_S04190	.	stem_loop	470	513	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04190	.	engineered_region	459	538	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04190	.	terminator	547	587	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_S04190	.	engineered_region	547	587	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04190	.	stop_codon	580	580	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04190	.	stem_loop	554	573	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04190	.	polyA_site	574	587	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04190	.	engineered_region	596	1533	.	+	0	ID=BBa_I7101;Name=BBa_I7101
BBa_S04190	.	ribosome_entry_site	658	670	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_I7101
BBa_S04190	.	ribosome_entry_site	664	667	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_S04190	.	engineered_region	658	670	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_S04190	.	ribosome_entry_site	658	670	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_S04190	.	terminator	1405	1484	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I7101
BBa_S04190	.	stem_loop	1416	1459	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04190	.	engineered_region	1405	1484	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04190	.	CDS	677	1396	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_I7101
BBa_S04190	.	CDS	677	1396	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_S04190	.	terminator	1493	1533	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I7101
BBa_S04190	.	engineered_region	1493	1533	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04190	.	stop_codon	1526	1526	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04190	.	stem_loop	1500	1519	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04190	.	polyA_site	1520	1533	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04190	.	promoter	596	649	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_I7101
BBa_S04190	.	promoter	615	620	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_S04190	.	non_covalent_binding_site	621	639	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_S04190	.	engineered_region	596	649	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_S04190	.	non_covalent_binding_site	596	614	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_S04190	.	promoter	638	643	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
>BBa_S04190
agtttattcttgacatggtcccacgcgcgtgggatactacgtcagtactagagaaagaggagaaatactagatgaccat
gattacgccaagcgcgcaattaaccctcactaaagggaacaaaagctggagctccaccgcggtggcggcagcactagag
ctagtggatcccccgggctgtagaaattcgatatcaagcttatcgataccgtcgacctcgagggggggcccggtaccca
attcgccctatagtgagtcgtattacgcgcgctcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgt
tacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgccct
tcccaacagttgcgcagcctgaattaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaa
acgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacact
ggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtg
atagagatactgagcactactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgt
cccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaaca
tacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcg
gttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaagg
ttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgat
acccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaataca
actataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaa
cattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttacca
gacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagt
ttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaac
gaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactgg
ctcaccttcgggtgggcctttctgcgtttata