##gff-version 3
##sequence-region BBa_S04185 1 2015
BBa_S04185	.	engineered_region	1	938	.	+	0	ID=BBa_I7101;Name=BBa_I7101
BBa_S04185	.	ribosome_entry_site	63	75	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_I7101
BBa_S04185	.	ribosome_entry_site	63	75	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_S04185	.	engineered_region	63	75	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_S04185	.	ribosome_entry_site	69	72	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_S04185	.	terminator	810	889	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I7101
BBa_S04185	.	stem_loop	821	864	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04185	.	engineered_region	810	889	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04185	.	CDS	82	801	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_I7101
BBa_S04185	.	CDS	82	801	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_S04185	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_I7101
BBa_S04185	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_S04185	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_S04185	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_S04185	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_S04185	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_S04185	.	terminator	898	938	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I7101
BBa_S04185	.	polyA_site	925	938	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04185	.	stop_codon	931	931	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04185	.	stem_loop	905	924	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04185	.	engineered_region	898	938	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04185	.	engineered_region	947	2015	.	+	0	ID=BBa_J04450;Name=BBa_J04450
BBa_S04185	.	terminator	1887	1966	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J04450
BBa_S04185	.	stem_loop	1898	1941	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04185	.	engineered_region	1887	1966	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04185	.	terminator	1975	2015	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J04450
BBa_S04185	.	polyA_site	2002	2015	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04185	.	stop_codon	2008	2008	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04185	.	stem_loop	1982	2001	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04185	.	engineered_region	1975	2015	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04185	.	CDS	1173	1853	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_J04450
BBa_S04185	.	CDS	1173	1847	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_S04185	.	engineered_tag	1854	1878	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_S04185	.	promoter	947	1146	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04450
BBa_S04185	.	non_covalent_binding_site	1112	1146	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_S04185	.	non_covalent_binding_site	1035	1072	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_S04185	.	promoter	1083	1088	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_S04185	.	start_codon	1119	1119	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_S04185	.	engineered_region	947	1146	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_S04185	.	CDS	947	1034	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_S04185	.	promoter	1107	1112	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_S04185	.	ribosome_entry_site	1155	1166	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04450
BBa_S04185	.	conserved_region	1159	1162	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_S04185
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacacaggaaagtact
agatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaa
attttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaa
ctacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatga
aacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaa
ctacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaa
gaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaa
agaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaaca
aaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagat
cccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactat
acaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgtt
gtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagca
atacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgg
gcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcg
tatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcttcctccg
aagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaagg
tgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgct
tgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctga
aactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccagga
ctcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatg
cagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaa
tgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagct
gccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaa
cgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatca
aataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagt
cacactggctcaccttcgggtgggcctttctgcgtttata